Restriction map for
pF1KA0946
(Circular) (Six-base) MAPSORT of: pF1KA0946.seq Check: 1013 from: 1 to: 5009
pF1KA0946 5009 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AccI GT'mk_AC
Cuts at: 1957 2047 1957
Size: 90 4919
Acc65I G'GTAC_C
Cuts at: 1991 2025 1991
Size: 34 4975
AclI AA'CG_TT
Cuts at: 4849 4849
Size: 5009
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2834 3266 4013 4281 2834
Size: 432 747 268 3562
Fragments arranged by size:
3562 747 432 268
AfeI AGC'GCT
Cuts at: 890 890
Size: 5009
AflIII A'CryG_T
Cuts at: 1684 2361 3465 4442 1684
Size: 677 1104 977 2251
Fragments arranged by size:
2251 1104 977 677
AgeI A'CCGG_T
Cuts at: 1446 3397 1446
Size: 1951 3058
AhdI GACnn_n'nnGTC
Cuts at: 972 1400 972
Size: 428 4581
AlwNI CAG_nnn'CTG
Cuts at: 1430 1987 2314 3881 1430
Size: 557 327 1567 2558
Fragments arranged by size:
2558 1567 557 327
ApaLI G'TGCA_C
Cuts at: 3779 3779
Size: 5009
ApoI r'AATT_y
Cuts at: 477 1006 1207 1492 1903 2013 4362 4618 477
Size: 529 201 285 411 110 2349 256 868
Fragments arranged by size:
2349 868 529 411 285 256 201 110
AseI AT'TA_AT
Cuts at: 20 562 20
Size: 542 4467
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5009
AvaI C'yCGr_G
Cuts at: 2029 2029
Size: 5009
BamHI G'GATC_C
Cuts at: 720 2034 720
Size: 1314 3695
BanI G'GyrC_C
Cuts at: 1991 2025 2439 2687 2722 1991
Size: 34 414 248 35 4278
Fragments arranged by size:
4278 414 248 35 34
BanII G_rGCy'C
Cuts at: 2023 3053 2023
Size: 1030 3979
BbeI G_GCGC'C
Cuts at: 2443 2691 2443
Size: 248 4761
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4975
BciVI GTATCCnnnnn_n'
Cuts at: 2907 3668 5009 2907
Size: 761 1341 2907
Fragments arranged by size:
2907 1341 761
BclI T'GATC_A
Cuts at: 926 1497 2529 926
Size: 571 1032 3406
Fragments arranged by size:
3406 1032 571
BfrBI ATG'CAT
Cuts at: 1170 1170
Size: 5009
BglII A'GATC_T
Cuts at: 2524 4561 2524
Size: 2037 2972
BlpI GC'TnA_GC
Cuts at: 2121 2121
Size: 5009
Bme1580I G_kGCm'C
Cuts at: 185 2634 2727 3783 185
Size: 2449 93 1056 1411
Fragments arranged by size:
2449 1411 1056 93
BmgBI CAC'GTC
Cuts at: 1226 1226
Size: 5009
BmrI ACTGGGnnnn_n'
Cuts at: 504 1626 2221 2302 2637 4781 504
Size: 1122 595 81 335 2144 732
Fragments arranged by size:
2144 1122 732 595 335 81
BmtI G_CTAG'C
Cuts at: 811 811
Size: 5009
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3376 3376
Size: 5009
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 869 869
Size: 5009
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2185 3064 3556 3854 2185
Size: 879 492 298 3340
Fragments arranged by size:
3340 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1627 4713 1627
Size: 3086 1923
BsaAI yAC'GTr
Cuts at: 1685 2362 1685
Size: 677 4332
BsaBI GATnn'nnATC
Cuts at: 6 2523 4560 6
Size: 2517 2037 455
Fragments arranged by size:
2517 2037 455
BsaHI Gr'CG_yC
Cuts at: 682 2440 2688 3390 4886 682
Size: 1758 248 702 1496 805
Fragments arranged by size:
1758 1496 805 702 248
BsaWI w'CCGG_w
Cuts at: 363 1446 2198 2719 3397 3671 3818 4229 363
Size: 1083 752 521 678 274 147 411 1143
Fragments arranged by size:
1143 1083 752 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 842 872 842
Size: 30 4979
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 133 376 133
Size: 243 4766
BseYI C'CCAG_C
Cuts at: 2434 3192 3769 4265 2434
Size: 758 577 496 3178
Fragments arranged by size:
3178 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 569 1553 569
Size: 984 4025
BsiEI CG_ry'CG
Cuts at: 104 2551 2597 3805 104
Size: 2447 46 1208 1308
Fragments arranged by size:
2447 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2023 2801 2991 3783 2023
Size: 778 190 792 3249
Fragments arranged by size:
3249 792 778 190
BsmI GAATG_Cn'
Cuts at: 1815 1815
Size: 5009
Bsp1286I G_dGCh'C
Cuts at: 185 2023 2634 2727 2801 2991 3053 3783 185
Size: 1838 611 93 74 190 62 730 1411
Fragments arranged by size:
1838 1411 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4229 4229
Size: 5009
BspHI T'CATG_A
Cuts at: 4185 4185
Size: 5009
BspMI ACCTGCnnnn'nnnn_
Cuts at: 334 2059 2273 2575 2956 334
Size: 1725 214 302 381 2387
Fragments arranged by size:
2387 1725 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3301 3355 4839 5004 50
Size: 3251 54 1484 165 55
Fragments arranged by size:
3251 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 487 1177 1503 2921 487
Size: 690 326 1418 2575
Fragments arranged by size:
2575 1418 690 326
BsrFI r'CCGG_y
Cuts at: 1446 3007 3188 3397 1446
Size: 1561 181 209 3058
Fragments arranged by size:
3058 1561 209 181
BssSI C'ACGA_G
Cuts at: 3280 3638 3280
Size: 358 4651
BstAPI GCAn_nnn'nTGC
Cuts at: 2386 2386
Size: 5009
BstBI TT'CG_AA
Cuts at: 439 3370 439
Size: 2931 2078
BstXI CCAn_nnnn'nTGG
Cuts at: 235 494 235
Size: 259 4750
BstYI r'GATC_y
Cuts at: 1 552 720 1332 1984 2034 2501 2524 2859
Size: 551 168 612 652 50 467 23 335
Cuts at: 2859 3105 4117 4203 4215 4561 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 652 612 551 467 449 346 335
246 168 86 50 23 12
BstZ17I GTA'TAC
Cuts at: 1958 1958
Size: 5009
BtgI C'CryG_G
Cuts at: 228 521 1510 1869 228
Size: 293 989 359 3368
Fragments arranged by size:
3368 989 359 293
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2959 3140 3303 2959
Size: 181 163 4665
Fragments arranged by size:
4665 181 163
BtsI GCAGTG_nn'
Cuts at: 785 809 2252 785
Size: 24 1443 3542
Fragments arranged by size:
3542 1443 24
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1446 1481 1446
Size: 35 4974
DraI TTT'AAA
Cuts at: 1833 2008 1833
Size: 175 4834
DraIII CAC_nnn'GTG
Cuts at: 715 715
Size: 5009
DrdI GACnn_nn'nnGTC
Cuts at: 2715 3573 2715
Size: 858 4151
EaeI y'GGCC_r
Cuts at: 2594 2768 3159 3186 4531 2594
Size: 174 391 27 1345 3072
Fragments arranged by size:
3072 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2594 2594
Size: 5009
EarI CTCTTCn'nnn_
Cuts at: 1352 3032 3242 1352
Size: 1680 210 3119
Fragments arranged by size:
3119 1680 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 157 3217 3527 3673 4819 157
Size: 3060 310 146 1146 347
Fragments arranged by size:
3060 1146 347 310 146
EcoICRI GAG'CTC
Cuts at: 2021 2021
Size: 5009
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 869 2834 3266 4013 4281 869
Size: 1965 432 747 268 1597
Fragments arranged by size:
1965 1597 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 471 2148 471
Size: 1677 3332
EcoRI G'AATT_C
Cuts at: 477 2013 477
Size: 1536 3473
EcoRV GAT'ATC
Cuts at: 1775 1775
Size: 5009
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3028 3028
Size: 5009
FspI TGC'GCA
Cuts at: 2790 2790
Size: 5009
HaeII r_GCGC'y
Cuts at: 892 2443 2691 3713 4692 892
Size: 1551 248 1022 979 1209
Fragments arranged by size:
1551 1209 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 958 990 1386 1418 1963 1995 2851 2881 2883
Size: 32 396 32 545 32 856 30 2
Cuts at: 2883 2913 958
Size: 30 3054
Fragments arranged by size:
3054 856 545 396 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 759 2048 759
Size: 1289 3720
HpaI GTT'AAC
Cuts at: 759 759
Size: 5009
KasI G'GCGC_C
Cuts at: 2439 2687 2439
Size: 248 4761
KpnI G_GTAC'C
Cuts at: 1995 2029 1995
Size: 34 4975
MfeI C'AATT_G
Cuts at: 620 620
Size: 5009
MluI A'CGCG_T
Cuts at: 4442 4442
Size: 5009
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 748 3680 3864 748
Size: 2932 184 1893
Fragments arranged by size:
2932 1893 184
MscI TGG'CCA
Cuts at: 2770 2770
Size: 5009
MslI CAynn'nnrTG
Cuts at: 136 233 266 526 1466 1738 1874 2998 3125
Size: 97 33 260 940 272 136 1124 127
Cuts at: 3125 136
Size: 2020
Fragments arranged by size:
2020 1124 940 272 260 136 127 97
33
MspA1I CmG'CkG
Cuts at: 114 1481 1998 2120 2434 2794 3807 4052 114
Size: 1367 517 122 314 360 1013 245 1071
Fragments arranged by size:
1367 1071 1013 517 360 314 245 122
NaeI GCC'GGC
Cuts at: 3190 3190
Size: 5009
NarI GG'CG_CC
Cuts at: 2440 2688 2440
Size: 248 4761
NcoI C'CATG_G
Cuts at: 228 521 1510 1869 228
Size: 293 989 359 3368
Fragments arranged by size:
3368 989 359 293
NgoMIV G'CCGG_C
Cuts at: 3188 3188
Size: 5009
NheI G'CTAG_C
Cuts at: 807 807
Size: 5009
NsiI A_TGCA'T
Cuts at: 1172 1172
Size: 5009
NspI r_CATG'y
Cuts at: 2062 2562 3469 2062
Size: 500 907 3602
Fragments arranged by size:
3602 907 500
PciI A'CATG_T
Cuts at: 3465 3465
Size: 5009
PflMI CCAn_nnn'nTGG
Cuts at: 1211 2001 1211
Size: 790 4219
PmeI GTTT'AAAC
Cuts at: 2008 2008
Size: 5009
PmlI CAC'GTG
Cuts at: 1685 1685
Size: 5009
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4148 4180 4148
Size: 32 4977
PpuMI rG'GwC_Cy
Cuts at: 471 471
Size: 5009
PsiI TTA'TAA
Cuts at: 224 224
Size: 5009
PstI C_TGCA'G
Cuts at: 1426 2056 2741 1426
Size: 630 685 3694
Fragments arranged by size:
3694 685 630
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5009
PvuII CAG'CTG
Cuts at: 1998 2434 2794 1998
Size: 436 360 4213
Fragments arranged by size:
4213 436 360
RsrII CG'GwC_CG
Cuts at: 3204 3204
Size: 5009
SacI G_AGCT'C
Cuts at: 2023 2023
Size: 5009
SalI G'TCGA_C
Cuts at: 2046 2046
Size: 5009
SapI GCTCTTCn'nnn_
Cuts at: 1352 3032 3242 1352
Size: 1680 210 3119
Fragments arranged by size:
3119 1680 210
SbfI CC_TGCA'GG
Cuts at: 2056 2056
Size: 5009
ScaI AGT'ACT
Cuts at: 4526 4526
Size: 5009
SfcI C'TryA_G
Cuts at: 33 1127 1422 2052 2737 3730 3921 33
Size: 1094 295 630 685 993 191 1121
Fragments arranged by size:
1121 1094 993 685 630 295 191
SfoI GGC'GCC
Cuts at: 2441 2689 2441
Size: 248 4761
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5009
SmaI CCC'GGG
Cuts at: 2031 2031
Size: 5009
SmlI C'TyrA_G
Cuts at: 2164 3079 3571 3833 2164
Size: 915 492 262 3340
Fragments arranged by size:
3340 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5009
SphI G_CATG'C
Cuts at: 2062 2562 2062
Size: 500 4509
SspI AAT'ATT
Cuts at: 1080 1887 1080
Size: 807 4202
StyI C'CwwG_G
Cuts at: 228 521 762 1185 1510 1646 1693 1869 2143
Size: 293 241 423 325 136 47 176 274
Cuts at: 2143 2497 228
Size: 354 2740
Fragments arranged by size:
2740 423 354 325 293 274 241 176
136 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 209 1503 1728 2592 2725 3184 3393 209
Size: 1294 225 864 133 459 209 1825
Fragments arranged by size:
1825 1294 864 459 225 209 133
TatI w'GTAC_w
Cuts at: 1131 4524 1131
Size: 3393 1616
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4526 4558 4526
Size: 32 4977
Tth111I GACn'n_nGTC
Cuts at: 289 2806 289
Size: 2517 2492
XbaI T'CTAG_A
Cuts at: 2040 2040
Size: 5009
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 226 2504 226
Size: 2278 2731
XmaI C'CCGG_G
Cuts at: 2029 2029
Size: 5009
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BstZ17I
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NsiI NspI PciI PflMI PmeI
PmlI PpiI PpuMI PsiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AleI AlfI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BglI Bpu10I BsiWI BsmBI
BsrGI BssHII BstEII Bsu36I ClaI EcoNI FseI FspAI
HindIII NdeI NotI NruI PacI PasI PfoI PshAI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI XmnI ZraI