Restriction map for
pF1KA0947
(Circular) (Six-base) MAPSORT of: pF1KA0947.seq Check: 1579 from: 1 to: 9911
pF1KA0947 9911 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 776 776
Size: 9911
AatII G_ACGT'C
Cuts at: 3568 3568
Size: 9911
AccI GT'mk_AC
Cuts at: 5626 6586 6741 6949 5626
Size: 960 155 208 8588
Fragments arranged by size:
8588 960 208 155
Acc65I G'GTAC_C
Cuts at: 3382 6927 3382
Size: 3545 6366
AclI AA'CG_TT
Cuts at: 9751 9751
Size: 9911
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 751 1766 3308 3950 4037 4802 5201 5492 5762
Size: 1015 1542 642 87 765 399 291 270
Cuts at: 5762 5884 7736 8168 8915 9183 751
Size: 122 1852 432 747 268 1479
Fragments arranged by size:
1852 1542 1479 1015 765 747 642 432
399 291 270 268 122 87
AflIII A'CryG_T
Cuts at: 4410 6209 7263 8367 9344 4410
Size: 1799 1054 1104 977 4977
Fragments arranged by size:
4977 1799 1104 1054 977
AgeI A'CCGG_T
Cuts at: 2794 8299 2794
Size: 5505 4406
AhdI GACnn_n'nnGTC
Cuts at: 5664 5664
Size: 9911
AleI CACnn'nnGTG
Cuts at: 2178 2486 2857 3127 2178
Size: 308 371 270 8962
Fragments arranged by size:
8962 371 308 270
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 551 3127 3468 3979 5273 6281 551
Size: 2576 341 511 1294 1008 4181
Fragments arranged by size:
4181 2576 1294 1008 511 341
AlwNI CAG_nnn'CTG
Cuts at: 1852 3520 4204 5055 5518 7216 8783 1852
Size: 1668 684 851 463 1698 1567 2980
Fragments arranged by size:
2980 1698 1668 1567 851 684 463
ApaI G_GGCC'C
Cuts at: 5397 5397
Size: 9911
ApaLI G'TGCA_C
Cuts at: 8681 8681
Size: 9911
ApoI r'AATT_y
Cuts at: 192 350 598 628 677 1060 1266 2644 2949
Size: 158 248 30 49 383 206 1378 305
Cuts at: 2949 3501 3924 3999 5346 5460 5980 6044 6400
Size: 552 423 75 1347 114 520 64 356
Cuts at: 6400 6418 6797 6915 9264 9520 192
Size: 18 379 118 2349 256 583
Fragments arranged by size:
2349 1378 1347 583 552 520 423 383
379 356 305 256 248 206 158 118
114 75 64 49 30 18
AseI AT'TA_AT
Cuts at: 20 217 1814 2419 6245 20
Size: 197 1597 605 3826 3686
Fragments arranged by size:
3826 3686 1597 605 197
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9911
AvaI C'yCGr_G
Cuts at: 139 1179 1432 1622 6931 139
Size: 1040 253 190 5309 3119
Fragments arranged by size:
5309 3119 1040 253 190
BamHI G'GATC_C
Cuts at: 6936 6936
Size: 9911
BanI G'GyrC_C
Cuts at: 135 2480 3301 3382 6927 7341 7589 7624 135
Size: 2345 821 81 3545 414 248 35 2422
Fragments arranged by size:
3545 2422 2345 821 414 248 81 35
BanII G_rGCy'C
Cuts at: 967 2230 2390 5397 6138 6925 7955 967
Size: 1263 160 3007 741 787 1030 2923
Fragments arranged by size:
3007 2923 1263 1030 787 741 160
BbeI G_GCGC'C
Cuts at: 139 7345 7593 139
Size: 7206 248 2457
Fragments arranged by size:
7206 2457 248
BbsI GAAGACnn'nnnn_
Cuts at: 613 1187 1609 2039 2554 4196 6434 613
Size: 574 422 430 515 1642 2238 4090
Fragments arranged by size:
4090 2238 1642 574 515 430 422
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1613 1647 1613
Size: 34 9877
BciVI GTATCCnnnnn_n'
Cuts at: 432 3351 5384 7809 8570 9911 432
Size: 2919 2033 2425 761 1341 432
Fragments arranged by size:
2919 2425 2033 1341 761 432
BclI T'GATC_A
Cuts at: 1068 1074 2256 2441 4686 4719 7431 1068
Size: 6 1182 185 2245 33 2712 3548
Fragments arranged by size:
3548 2712 2245 1182 185 33 6
BfrBI ATG'CAT
Cuts at: 1634 1634
Size: 9911
BglII A'GATC_T
Cuts at: 1092 3078 3184 7426 9463 1092
Size: 1986 106 4242 2037 1540
Fragments arranged by size:
4242 2037 1986 1540 106
BlpI GC'TnA_GC
Cuts at: 7023 7023
Size: 9911
Bme1580I G_kGCm'C
Cuts at: 1151 5397 6885 7536 7629 8685 1151
Size: 4246 1488 651 93 1056 2377
Fragments arranged by size:
4246 2377 1488 1056 651 93
BmgBI CAC'GTC
Cuts at: 5176 5176
Size: 9911
BmrI ACTGGGnnnn_n'
Cuts at: 391 2037 4489 5128 5543 5672 7123 7204 7539
Size: 1646 2452 639 415 129 1451 81 335
Cuts at: 7539 9683 391
Size: 2144 619
Fragments arranged by size:
2452 2144 1646 1451 639 619 415 335
129 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 506 4113 8278 506
Size: 3607 4165 2139
Fragments arranged by size:
4165 3607 2139
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 810 951 2909 3072 4463 5171 5720 810
Size: 141 1958 163 1391 708 549 5001
Fragments arranged by size:
5001 1958 1391 708 549 163 141
Bpu10I CC'TnA_GC
Cuts at: 512 6886 512
Size: 6374 3537
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1039 1889 2244 2760 3664 6324 6795 7087 7966
Size: 850 355 516 904 2660 471 292 879
Cuts at: 7966 8458 8756 1039
Size: 492 298 2194
Fragments arranged by size:
2660 2194 904 879 850 516 492 471
355 298 292
BsaI GGTCTCn'nnnn_
Cuts at: 115 3531 9615 115
Size: 3416 6084 411
Fragments arranged by size:
6084 3416 411
BsaAI yAC'GTr
Cuts at: 7264 7264
Size: 9911
BsaBI GATnn'nnATC
Cuts at: 6 443 1073 1091 4349 6663 7425 9462 6
Size: 437 630 18 3258 2314 762 2037 455
Fragments arranged by size:
3258 2314 2037 762 630 455 437 18
BsaHI Gr'CG_yC
Cuts at: 136 3565 7342 7590 8292 9788 136
Size: 3429 3777 248 702 1496 259
Fragments arranged by size:
3777 3429 1496 702 259 248
BsaWI w'CCGG_w
Cuts at: 2175 2794 6174 7100 7621 8299 8573 8720 9131
Size: 619 3380 926 521 678 274 147 411
Cuts at: 9131 2175
Size: 2955
Fragments arranged by size:
3380 2955 926 678 619 521 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 840 870 3174 3204 4926 4956 840
Size: 30 2304 30 1722 30 5795
Fragments arranged by size:
5795 2304 1722 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 347 1121 4029 5009 5174 5234 6910 347
Size: 774 2908 980 165 60 1676 3348
Fragments arranged by size:
3348 2908 1676 980 774 165 60
BseYI C'CCAG_C
Cuts at: 6781 7336 8094 8671 9167 6781
Size: 555 758 577 496 7525
Fragments arranged by size:
7525 758 577 555 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 913 1643 3058 5813 913
Size: 730 1415 2755 5011
Fragments arranged by size:
5011 2755 1415 730
BsiEI CG_ry'CG
Cuts at: 104 7453 7499 8707 104
Size: 7349 46 1208 1308
Fragments arranged by size:
7349 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 967 2390 4228 4615 6138 6892 6925 7703 7893
Size: 1423 1838 387 1523 754 33 778 190
Cuts at: 7893 8685 967
Size: 792 2193
Fragments arranged by size:
2193 1838 1523 1423 792 778 754 387
190 33
BsmI GAATG_Cn'
Cuts at: 478 3404 6336 478
Size: 2926 2932 4053
Fragments arranged by size:
4053 2932 2926
BsmBI CGTCTCn'nnnn_
Cuts at: 3413 5246 3413
Size: 1833 8078
Bsp1286I G_dGCh'C
Cuts at: 967 1151 2230 2390 4228 4615 5397 6138 6885
Size: 184 1079 160 1838 387 782 741 747
Cuts at: 6885 6892 6925 7536 7629 7703 7893 7955 8685
Size: 7 33 611 93 74 190 62 730
Cuts at: 8685 967
Size: 2193
Fragments arranged by size:
2193 1838 1079 782 747 741 730 611
387 190 184 160 93 74 62 33
7
BspEI T'CCGG_A
Cuts at: 6174 9131 6174
Size: 2957 6954
BspHI T'CATG_A
Cuts at: 1071 9087 1071
Size: 8016 1895
BspMI ACCTGCnnnn'nnnn_
Cuts at: 776 813 4202 5481 6961 7175 7477 7858 776
Size: 37 3389 1279 1480 214 302 381 2829
Fragments arranged by size:
3389 2829 1480 1279 381 302 214 37
BsrBI CCG'CTC
Cuts at: 50 8203 8257 9741 9906 50
Size: 8153 54 1484 165 55
Fragments arranged by size:
8153 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2365 2579 3945 6260 6578 7823 2365
Size: 214 1366 2315 318 1245 4453
Fragments arranged by size:
4453 2315 1366 1245 318 214
BsrFI r'CCGG_y
Cuts at: 2794 7909 8090 8299 2794
Size: 5115 181 209 4406
Fragments arranged by size:
5115 4406 209 181
BssSI C'ACGA_G
Cuts at: 458 5435 8182 8540 458
Size: 4977 2747 358 1829
Fragments arranged by size:
4977 2747 1829 358
BstAPI GCAn_nnn'nTGC
Cuts at: 5518 6580 7288 5518
Size: 1062 708 8141
Fragments arranged by size:
8141 1062 708
BstBI TT'CG_AA
Cuts at: 8272 8272
Size: 9911
BstXI CCAn_nnnn'nTGG
Cuts at: 3081 6810 3081
Size: 3729 6182
BstYI r'GATC_y
Cuts at: 1 385 1092 2373 3078 3184 5190 6494 6806
Size: 384 707 1281 705 106 2006 1304 312
Cuts at: 6806 6936 7403 7426 7761 8007 9019 9105 9117
Size: 130 467 23 335 246 1012 86 12
Cuts at: 9117 9463 1
Size: 346 449
Fragments arranged by size:
2006 1304 1281 1012 707 705 467 449
384 346 335 312 246 130 106 86
23 12
BstZ17I GTA'TAC
Cuts at: 6587 6742 6587
Size: 155 9756
Bsu36I CC'TnA_GG
Cuts at: 3068 5339 3068
Size: 2271 7640
BtgI C'CryG_G
Cuts at: 917 5155 6733 917
Size: 4238 1578 4095
Fragments arranged by size:
4238 4095 1578
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 177 7861 8042 8205 177
Size: 7684 181 163 1883
Fragments arranged by size:
7684 1883 181 163
BtsI GCAGTG_nn'
Cuts at: 2274 2654 3041 3111 3462 4608 5693 7154 2274
Size: 380 387 70 351 1146 1085 1461 5031
Fragments arranged by size:
5031 1461 1146 1085 387 380 351 70
ClaI AT'CG_AT
Cuts at: 4770 4770
Size: 9911
DraI TTT'AAA
Cuts at: 392 425 2147 4004 6910 392
Size: 33 1722 1857 2906 3393
Fragments arranged by size:
3393 2906 1857 1722 33
DraIII CAC_nnn'GTG
Cuts at: 330 3866 4430 4432 5308 330
Size: 3536 564 2 876 4933
Fragments arranged by size:
4933 3536 876 564 2
DrdI GACnn_nn'nnGTC
Cuts at: 7617 8475 7617
Size: 858 9053
EaeI y'GGCC_r
Cuts at: 1854 5106 5158 5261 5292 5316 7496 7670 8061
Size: 3252 52 103 31 24 2180 174 391
Cuts at: 8061 8088 9433 1854
Size: 27 1345 2332
Fragments arranged by size:
3252 2332 2180 1345 391 174 103 52
31 27 24
EagI C'GGCC_G
Cuts at: 7496 7496
Size: 9911
EarI CTCTTCn'nnn_
Cuts at: 297 329 401 475 1139 2010 2257 3800 7934
Size: 32 72 74 664 871 247 1543 4134
Cuts at: 7934 8144 297
Size: 210 2064
Fragments arranged by size:
4134 2064 1543 871 664 247 210 74
72 32
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 166 5432 6837 8119 8429 8575 9721 166
Size: 5266 1405 1282 310 146 1146 356
Fragments arranged by size:
5266 1405 1282 1146 356 310 146
EcoICRI GAG'CTC
Cuts at: 965 2388 6136 6923 965
Size: 1423 3748 787 3953
Fragments arranged by size:
3953 3748 1423 787
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 751 810 951 1766 2909 3072 3308 3950 4037
Size: 59 141 815 1143 163 236 642 87
Cuts at: 4037 4463 4802 5171 5201 5492 5720 5762 5884
Size: 426 339 369 30 291 228 42 122
Cuts at: 5884 7736 8168 8915 9183 751
Size: 1852 432 747 268 1479
Fragments arranged by size:
1852 1479 1143 815 747 642 432 426
369 339 291 268 236 228 163 141
122 87 59 42 30
EcoNI CCTnn'n_nnAGG
Cuts at: 776 776
Size: 9911
EcoO109I rG'GnC_Cy
Cuts at: 881 3198 4993 5166 5335 5393 5470 5666 7050
Size: 2317 1795 173 169 58 77 196 1384
Cuts at: 7050 881
Size: 3742
Fragments arranged by size:
3742 2317 1795 1384 196 173 169 77
58
EcoRI G'AATT_C
Cuts at: 2644 5346 6915 2644
Size: 2702 1569 5640
Fragments arranged by size:
5640 2702 1569
EcoRV GAT'ATC
Cuts at: 5069 6056 5069
Size: 987 8924
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 358 951 3808 7930 358
Size: 593 2857 4122 2339
Fragments arranged by size:
4122 2857 2339 593
FspI TGC'GCA
Cuts at: 7692 7692
Size: 9911
HaeII r_GCGC'y
Cuts at: 139 7345 7593 8615 9594 139
Size: 7206 248 1022 979 456
Fragments arranged by size:
7206 1022 979 456 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1234 1266 1630 1662 1933 1965 2398 2430 2541
Size: 32 364 32 271 32 433 32 111
Cuts at: 2541 2573 3070 3102 3555 3587 4659 4691 5064
Size: 32 497 32 453 32 1072 32 373
Cuts at: 5064 5096 5650 5682 7753 7783 7785 7815 1234
Size: 32 554 32 2071 30 2 30 3330
Fragments arranged by size:
3330 2071 1072 554 497 453 433 373
364 271 111 32 32 32 32 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 260 2804 5621 6950 260
Size: 2544 2817 1329 3221
Fragments arranged by size:
3221 2817 2544 1329
HindIII A'AGCT_T
Cuts at: 6815 6815
Size: 9911
HpaI GTT'AAC
Cuts at: 260 260
Size: 9911
KasI G'GCGC_C
Cuts at: 135 7341 7589 135
Size: 7206 248 2457
Fragments arranged by size:
7206 2457 248
KpnI G_GTAC'C
Cuts at: 3386 6931 3386
Size: 3545 6366
MfeI C'AATT_G
Cuts at: 1106 6640 1106
Size: 5534 4377
MluI A'CGCG_T
Cuts at: 9344 9344
Size: 9911
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 883 8582 8766 883
Size: 7699 184 2028
Fragments arranged by size:
7699 2028 184
MscI TGG'CCA
Cuts at: 1856 5108 5160 5318 7672 1856
Size: 3252 52 158 2354 4095
Fragments arranged by size:
4095 3252 2354 158 52
MslI CAynn'nnrTG
Cuts at: 2178 2486 2857 3104 3127 4290 4495 4847 5918
Size: 308 371 247 23 1163 205 352 1071
Cuts at: 5918 7900 8027 2178
Size: 1982 127 4062
Fragments arranged by size:
4062 1982 1163 1071 371 352 308 247
205 127 23
MspA1I CmG'CkG
Cuts at: 3167 4075 4109 5740 6863 7022 7336 7696 8709
Size: 908 34 1631 1123 159 314 360 1013
Cuts at: 8709 8954 3167
Size: 245 4124
Fragments arranged by size:
4124 1631 1123 1013 908 360 314 245
159 34
NaeI GCC'GGC
Cuts at: 8092 8092
Size: 9911
NarI GG'CG_CC
Cuts at: 136 7342 7590 136
Size: 7206 248 2457
Fragments arranged by size:
7206 2457 248
NcoI C'CATG_G
Cuts at: 917 5155 6733 917
Size: 4238 1578 4095
Fragments arranged by size:
4238 4095 1578
NdeI CA'TA_TG
Cuts at: 693 693
Size: 9911
NgoMIV G'CCGG_C
Cuts at: 8090 8090
Size: 9911
NruI TCG'CGA
Cuts at: 162 162
Size: 9911
NsiI A_TGCA'T
Cuts at: 1636 1636
Size: 9911
NspI r_CATG'y
Cuts at: 902 4051 4414 6213 6574 6715 6964 7464 8371
Size: 3149 363 1799 361 141 249 500 907
Cuts at: 8371 902
Size: 2442
Fragments arranged by size:
3149 2442 1799 907 500 363 361 249
141
PasI CC'CwG_GG
Cuts at: 1681 5722 1681
Size: 4041 5870
PciI A'CATG_T
Cuts at: 4410 6209 8367 4410
Size: 1799 2158 5954
Fragments arranged by size:
5954 2158 1799
PfoI T'CCnGG_A
Cuts at: 4440 6809 4440
Size: 2369 7542
PmeI GTTT'AAAC
Cuts at: 6910 6910
Size: 9911
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 728 760 9050 9082 728
Size: 32 8290 32 1557
Fragments arranged by size:
8290 1557 32 32
PpuMI rG'GwC_Cy
Cuts at: 881 5166 5470 5666 881
Size: 4285 304 196 5126
Fragments arranged by size:
5126 4285 304 196
PshAI GACnn'nnGTC
Cuts at: 6776 6776
Size: 9911
PsiI TTA'TAA
Cuts at: 4324 4336 4733 4324
Size: 12 397 9502
Fragments arranged by size:
9502 397 12
PspOMI G'GGCC_C
Cuts at: 5393 5393
Size: 9911
PspXI vC'TCGA_Gb
Cuts at: 1622 1622
Size: 9911
PstI C_TGCA'G
Cuts at: 188 293 2165 3036 3457 5214 5252 5700 6091
Size: 105 1872 871 421 1757 38 448 391
Cuts at: 6091 6958 7643 188
Size: 867 685 2456
Fragments arranged by size:
2456 1872 1757 871 867 685 448 421
391 105 38
PvuI CG_AT'CG
Cuts at: 104 104
Size: 9911
PvuII CAG'CTG
Cuts at: 3167 4075 5740 6863 7336 7696 3167
Size: 908 1665 1123 473 360 5382
Fragments arranged by size:
5382 1665 1123 908 473 360
RsrII CG'GwC_CG
Cuts at: 8106 8106
Size: 9911
SacI G_AGCT'C
Cuts at: 967 2390 6138 6925 967
Size: 1423 3748 787 3953
Fragments arranged by size:
3953 3748 1423 787
SalI G'TCGA_C
Cuts at: 6948 6948
Size: 9911
SanDI GG'GwC_CC
Cuts at: 5666 5666
Size: 9911
SapI GCTCTTCn'nnn_
Cuts at: 2257 7934 8144 2257
Size: 5677 210 4024
Fragments arranged by size:
5677 4024 210
SbfI CC_TGCA'GG
Cuts at: 6958 6958
Size: 9911
ScaI AGT'ACT
Cuts at: 1056 2472 3594 9428 1056
Size: 1416 1122 5834 1539
Fragments arranged by size:
5834 1539 1416 1122
SexAI A'CCwGG_T
Cuts at: 771 4418 771
Size: 3647 6264
SfcI C'TryA_G
Cuts at: 33 184 289 361 2161 2186 2639 2807 3032
Size: 151 105 72 1800 25 453 168 225
Cuts at: 3032 3453 3524 4946 5210 5248 5696 6087 6150
Size: 421 71 1422 264 38 448 391 63
Cuts at: 6150 6954 7639 8632 8823 33
Size: 804 685 993 191 1121
Fragments arranged by size:
1800 1422 1121 993 804 685 453 448
421 391 264 225 191 168 151 105
72 71 63 38 25
SfoI GGC'GCC
Cuts at: 137 7343 7591 137
Size: 7206 248 2457
Fragments arranged by size:
7206 2457 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9911
SmaI CCC'GGG
Cuts at: 141 1181 6933 141
Size: 1040 5752 3119
Fragments arranged by size:
5752 3119 1040
SmlI C'TyrA_G
Cuts at: 1018 1432 1622 1904 2223 2739 3643 6303 6774
Size: 414 190 282 319 516 904 2660 471
Cuts at: 6774 7066 7981 8473 8735 1018
Size: 292 915 492 262 2194
Fragments arranged by size:
2660 2194 915 904 516 492 471 414
319 292 282 262 190
SpeI A'CTAG_T
Cuts at: 67 67
Size: 9911
SphI G_CATG'C
Cuts at: 6715 6964 7464 6715
Size: 249 500 9162
Fragments arranged by size:
9162 500 249
SspI AAT'ATT
Cuts at: 5424 6681 5424
Size: 1257 8654
StuI AGG'CCT
Cuts at: 6832 6832
Size: 9911
StyI C'CwwG_G
Cuts at: 917 1710 2012 4079 5038 5155 6733 7045 7399
Size: 793 302 2067 959 117 1578 312 354
Cuts at: 7399 917
Size: 3429
Fragments arranged by size:
3429 2067 1578 959 793 354 312 302
117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2315 2619 7494 7627 8086 8295 2315
Size: 304 4875 133 459 209 3931
Fragments arranged by size:
4875 3931 459 304 209 133
TatI w'GTAC_w
Cuts at: 1029 1054 1590 2470 3592 9426 1029
Size: 25 536 880 1122 5834 1514
Fragments arranged by size:
5834 1514 1122 880 536 25
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1168 1200 3174 3206 5358 5390 5424 5456 9428
Size: 32 1974 32 2152 32 34 32 3972
Cuts at: 9428 9460 1168
Size: 32 1619
Fragments arranged by size:
3972 2152 1974 1619 34 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1252 3135 4420 7708 1252
Size: 1883 1285 3288 3455
Fragments arranged by size:
3455 3288 1883 1285
XbaI T'CTAG_A
Cuts at: 1873 6942 1873
Size: 5069 4842
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2050 7406 2050
Size: 5356 4555
XhoI C'TCGA_G
Cuts at: 1432 1622 1432
Size: 190 9721
XmaI C'CCGG_G
Cuts at: 139 1179 6931 139
Size: 1040 5752 3119
Fragments arranged by size:
5752 3119 1040
XmnI GAAnn'nnTTC
Cuts at: 1094 2714 5772 6170 6659 1094
Size: 1620 3058 398 489 4346
Fragments arranged by size:
4346 3058 1620 489 398
ZraI GAC'GTC
Cuts at: 3566 3566
Size: 9911
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NruI NsiI NspI PasI
PciI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AloI AscI AvrII BaeI BbvCI BglI
BmtI BsiWI BsrGI BssHII BstEII CspCI FseI FspAI
NheI NotI PacI PflMI PmlI PsrI SacII SfiI
SgrAI SnaBI SrfI SwaI