Restriction map for
pF1KA0949
(Circular) (Six-base) MAPSORT of: pF1KA0949.seq Check: 7679 from: 1 to: 7748
pF1KA0949 7748 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2902 3274 3343 3683 3993 4023 2902
Size: 372 69 340 310 30 6627
Fragments arranged by size:
6627 372 340 310 69 30
AccI GT'mk_AC
Cuts at: 4786 4786
Size: 7748
Acc65I G'GTAC_C
Cuts at: 4764 4764
Size: 7748
AclI AA'CG_TT
Cuts at: 7588 7588
Size: 7748
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 518 587 779 1085 1484 1774 2065 2290
Size: 310 69 192 306 399 290 291 225
Cuts at: 2290 2467 2485 3259 3447 4721 5573 6005 6358
Size: 177 18 774 188 1274 852 432 353
Cuts at: 6358 7020 208
Size: 662 936
Fragments arranged by size:
1274 936 852 774 662 432 399 353
310 306 291 290 225 192 188 177
69 18
AfeI AGC'GCT
Cuts at: 3322 3322
Size: 7748
AflII C'TTAA_G
Cuts at: 2722 2722
Size: 7748
AflIII A'CryG_T
Cuts at: 5100 6900 7181 5100
Size: 1800 281 5667
Fragments arranged by size:
5667 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 524 524
Size: 7748
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 245 3010 245
Size: 2765 4983
AlwNI CAG_nnn'CTG
Cuts at: 1317 1462 2043 2133 3385 3660 5053 6491 1317
Size: 145 581 90 1252 275 1393 1438 2574
Fragments arranged by size:
2574 1438 1393 1252 581 275 145 90
ApaI G_GGCC'C
Cuts at: 4291 4405 4291
Size: 114 7634
ApaLI G'TGCA_C
Cuts at: 844 1764 2812 6586 844
Size: 920 1048 3774 2006
Fragments arranged by size:
3774 2006 1048 920
ApoI r'AATT_y
Cuts at: 502 620 983 1156 1513 2707 3139 3566 3913
Size: 118 363 173 357 1194 432 427 347
Cuts at: 3913 3952 4081 4752 7101 7357 502
Size: 39 129 671 2349 256 893
Fragments arranged by size:
2349 1194 893 671 432 427 363 357
347 256 173 129 118 39
AseI AT'TA_AT
Cuts at: 20 20
Size: 7748
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7748
AvaI C'yCGr_G
Cuts at: 2531 3274 3946 4250 4642 4768 2531
Size: 743 672 304 392 126 5511
Fragments arranged by size:
5511 743 672 392 304 126
BamHI G'GATC_C
Cuts at: 4773 4773
Size: 7748
BanI G'GyrC_C
Cuts at: 1645 3018 3181 3487 3981 4764 5178 5426 5461
Size: 1373 163 306 494 783 414 248 35
Cuts at: 5461 1645
Size: 3932
Fragments arranged by size:
3932 1373 783 494 414 306 248 163
35
BanII G_rGCy'C
Cuts at: 632 797 1325 2153 2522 2984 3503 3764 3863
Size: 165 528 828 369 462 519 261 99
Cuts at: 3863 4291 4405 4762 5792 632
Size: 428 114 357 1030 2588
Fragments arranged by size:
2588 1030 828 528 519 462 428 369
357 261 165 114 99
BbeI G_GCGC'C
Cuts at: 1649 5182 5430 1649
Size: 3533 248 3967
Fragments arranged by size:
3967 3533 248
BbsI GAAGACnn'nnnn_
Cuts at: 453 1438 1762 2278 2512 2684 3552 4116 4664
Size: 985 324 516 234 172 868 564 548
Cuts at: 4664 4727 453
Size: 63 3474
Fragments arranged by size:
3474 985 868 564 548 516 324 234
172 63
BbvCI CC'TCA_GC
Cuts at: 788 3820 4233 788
Size: 3032 413 4303
Fragments arranged by size:
4303 3032 413
BciVI GTATCCnnnnn_n'
Cuts at: 2799 3883 3904 5646 6702 7748 2799
Size: 1084 21 1742 1056 1046 2799
Fragments arranged by size:
2799 1742 1084 1056 1046 21
BclI T'GATC_A
Cuts at: 1007 5268 1007
Size: 4261 3487
BglI GCCn_nnn'nGGC
Cuts at: 1390 1390
Size: 7748
BglII A'GATC_T
Cuts at: 5263 7300 5263
Size: 2037 5711
BlpI GC'TnA_GC
Cuts at: 605 4618 4860 605
Size: 4013 242 3493
Fragments arranged by size:
4013 3493 242
Bme1580I G_kGCm'C
Cuts at: 848 1768 2816 3023 3490 3624 4291 4405 5373
Size: 920 1048 207 467 134 667 114 968
Cuts at: 5373 5466 6590 848
Size: 93 1124 2006
Fragments arranged by size:
2006 1124 1048 968 920 667 467 207
134 114 93
BmgBI CAC'GTC
Cuts at: 3639 3639
Size: 7748
BmrI ACTGGGnnnn_n'
Cuts at: 1869 2639 3660 4960 5041 5376 7520 1869
Size: 770 1021 1300 81 335 2144 2097
Fragments arranged by size:
2144 2097 1300 1021 770 335 81
BmtI G_CTAG'C
Cuts at: 3298 3298
Size: 7748
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 500 2076 6115 500
Size: 1576 4039 2133
Fragments arranged by size:
4039 2133 1576
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 238 610 664 748 955 1186 1228 1240 1291
Size: 372 54 84 207 231 42 12 51
Cuts at: 1291 1339 1429 1576 1795 1866 2101 2155 2479
Size: 48 90 147 219 71 235 54 324
Cuts at: 2479 2684 2953 3094 3607 4461 4609 238
Size: 205 269 141 513 854 148 3377
Fragments arranged by size:
3377 854 513 372 324 269 235 231
219 207 205 148 147 141 90 84
71 54 54 51 48 42 12
Bpu10I CC'TnA_GC
Cuts at: 548 788 3820 4233 548
Size: 240 3032 413 4063
Fragments arranged by size:
4063 3032 413 240
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 371 1309 2216 4117 4924 5803 6517 6815 371
Size: 938 907 1901 807 879 714 298 1304
Fragments arranged by size:
1901 1304 938 907 879 807 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1441 2971 3844 7452 1441
Size: 1530 873 3608 1737
Fragments arranged by size:
3608 1737 1530 873
BsaAI yAC'GTr
Cuts at: 1763 2879 4445 5101 1763
Size: 1116 1566 656 4410
Fragments arranged by size:
4410 1566 1116 656
BsaBI GATnn'nnATC
Cuts at: 6 5262 7299 6
Size: 5256 2037 455
Fragments arranged by size:
5256 2037 455
BsaHI Gr'CG_yC
Cuts at: 1646 2718 2823 4321 5179 5427 6129 7625 1646
Size: 1072 105 1498 858 248 702 1496 1769
Fragments arranged by size:
1769 1498 1496 1072 858 702 248 105
BsaWI w'CCGG_w
Cuts at: 2522 3836 4937 5458 6136 6547 6694 6968 2522
Size: 1314 1101 521 678 411 147 274 3302
Fragments arranged by size:
3302 1314 1101 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1354 1384 4079 4109 1354
Size: 30 2695 30 4993
Fragments arranged by size:
4993 2695 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 805 973 976 1225 1321 1483 1573 1863 4072
Size: 168 3 249 96 162 90 290 2209
Cuts at: 4072 4221 805
Size: 149 4332
Fragments arranged by size:
4332 2209 290 249 168 162 149 96
90 3
BseYI C'CCAG_C
Cuts at: 830 1177 2983 3050 3301 3323 3683 3784 4482
Size: 347 1806 67 251 22 360 101 698
Cuts at: 4482 4501 5173 5931 6596 7004 830
Size: 19 672 758 665 408 1574
Fragments arranged by size:
1806 1574 758 698 672 665 408 360
347 251 101 67 22 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1379 2501 3435 3794 4051 1379
Size: 1122 934 359 257 5076
Fragments arranged by size:
5076 1122 934 359 257
BsiEI CG_ry'CG
Cuts at: 104 2629 4611 5290 5336 6566 104
Size: 2525 1982 679 46 1230 1286
Fragments arranged by size:
2525 1982 1286 1230 679 46
BsiHKAI G_wGCw'C
Cuts at: 632 797 848 1304 1325 1768 1997 2153 2522
Size: 165 51 456 21 443 229 156 369
Cuts at: 2522 2639 2816 2875 4457 4762 5540 5730 6590
Size: 117 177 59 1582 305 778 190 860
Cuts at: 6590 632
Size: 1790
Fragments arranged by size:
1790 1582 860 778 456 443 369 305
229 190 177 165 156 117 59 51
21
BsmI GAATG_Cn'
Cuts at: 253 1913 2115 253
Size: 1660 202 5886
Fragments arranged by size:
5886 1660 202
Bsp1286I G_dGCh'C
Cuts at: 632 797 848 1304 1325 1768 1997 2153 2522
Size: 165 51 456 21 443 229 156 369
Cuts at: 2522 2639 2816 2875 2984 3023 3490 3503 3624
Size: 117 177 59 109 39 467 13 121
Cuts at: 3624 3764 3863 4291 4405 4457 4762 5373 5466
Size: 140 99 428 114 52 305 611 93
Cuts at: 5466 5540 5730 5792 6590 632
Size: 74 190 62 798 1790
Fragments arranged by size:
1790 798 611 467 456 443 428 369
305 229 190 177 165 156 140 121
117 114 109 99 93 74 62 59
52 51 39 21 13
BspEI T'CCGG_A
Cuts at: 3836 3836
Size: 7748
BspHI T'CATG_A
Cuts at: 2328 3599 6180 2328
Size: 1271 2581 3896
Fragments arranged by size:
3896 2581 1271
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2210 2415 2902 3274 3343 3683 3993 4023 4798
Size: 205 487 372 69 340 310 30 775
Cuts at: 4798 5012 5314 5695 2210
Size: 214 302 381 4263
Fragments arranged by size:
4263 775 487 381 372 340 310 302
214 205 69 30
BsrBI CCG'CTC
Cuts at: 50 1830 3618 4635 6040 6094 7578 7743 50
Size: 1780 1788 1017 1405 54 1484 165 55
Fragments arranged by size:
1788 1780 1484 1405 1017 165 55 54
BsrDI GCAATG_nn'
Cuts at: 5660 5660
Size: 7748
BsrFI r'CCGG_y
Cuts at: 3129 4673 5746 5927 3129
Size: 1544 1073 181 4950
Fragments arranged by size:
4950 1544 1073 181
BsrGI T'GTAC_A
Cuts at: 1662 1662
Size: 7748
BssSI C'ACGA_G
Cuts at: 955 1303 4369 6019 6727 955
Size: 348 3066 1650 708 1976
Fragments arranged by size:
3066 1976 1650 708 348
BstAPI GCAn_nnn'nTGC
Cuts at: 1908 2097 3830 5125 1908
Size: 189 1733 1295 4531
Fragments arranged by size:
4531 1733 1295 189
BstBI TT'CG_AA
Cuts at: 6109 6109
Size: 7748
BstEII G'GTnAC_C
Cuts at: 3327 3327
Size: 7748
BstXI CCAn_nnnn'nTGG
Cuts at: 3736 4084 4470 3736
Size: 348 386 7014
Fragments arranged by size:
7014 386 348
BstYI r'GATC_y
Cuts at: 1 208 429 1033 1472 4217 4773 5240 5263
Size: 207 221 604 439 2745 556 467 23
Cuts at: 5263 5598 5844 6150 6162 6248 7300 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
2745 1052 604 556 467 449 439 335
306 246 221 207 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 4302 4302
Size: 7748
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1018 5698 5879 6042 1018
Size: 4680 181 163 2724
Fragments arranged by size:
4680 2724 181 163
BtsI GCAGTG_nn'
Cuts at: 313 1840 1991 4991 313
Size: 1527 151 3000 3070
Fragments arranged by size:
3070 3000 1527 151
DraI TTT'AAA
Cuts at: 4747 4747
Size: 7748
DraIII CAC_nnn'GTG
Cuts at: 2789 2789
Size: 7748
DrdI GACnn_nn'nnGTC
Cuts at: 4704 5454 6798 4704
Size: 750 1344 5654
Fragments arranged by size:
5654 1344 750
EaeI y'GGCC_r
Cuts at: 4578 4608 5333 5507 5898 5925 7270 4578
Size: 30 725 174 391 27 1345 5056
Fragments arranged by size:
5056 1345 725 391 174 30 27
EagI C'GGCC_G
Cuts at: 4608 5333 4608
Size: 725 7023
EarI CTCTTCn'nnn_
Cuts at: 419 794 1121 1439 1460 1529 2165 3608 4008
Size: 375 327 318 21 69 636 1443 400
Cuts at: 4008 5771 5981 419
Size: 1763 210 2186
Fragments arranged by size:
2186 1763 1443 636 400 375 327 318
210 69 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 685 1681 2517 2604 4563 5956 6698 6844 7558
Size: 996 836 87 1959 1393 742 146 714
Cuts at: 7558 685
Size: 875
Fragments arranged by size:
1959 1393 996 875 836 742 714 146
87
EcoICRI GAG'CTC
Cuts at: 630 795 1323 2151 2520 4760 630
Size: 165 528 828 369 2240 3618
Fragments arranged by size:
3618 2240 828 528 369 165
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 238 518 587 610 664 748 779 955
Size: 30 280 69 23 54 84 31 176
Cuts at: 955 1085 1186 1228 1240 1291 1339 1429 1484
Size: 130 101 42 12 51 48 90 55
Cuts at: 1484 1576 1774 1795 1866 2065 2101 2155 2290
Size: 92 198 21 71 199 36 54 135
Cuts at: 2290 2467 2479 2485 2684 2953 3094 3259 3447
Size: 177 12 6 199 269 141 165 188
Cuts at: 3447 3607 4461 4609 4721 5573 6005 6358 7020
Size: 160 854 148 112 852 432 353 662
Cuts at: 7020 208
Size: 936
Fragments arranged by size:
936 854 852 662 432 353 280 269
199 199 198 188 177 176 165 160
148 141 135 130 112 101 92 90
84 71 69 55 54 54 51 48
42 36 31 30 23 21 12 12
6
EcoNI CCTnn'n_nnAGG
Cuts at: 220 2461 4372 220
Size: 2241 1911 3596
Fragments arranged by size:
3596 2241 1911
EcoO109I rG'GnC_Cy
Cuts at: 225 1800 3582 4241 4288 4401 4498 4697 4887
Size: 1575 1782 659 47 113 97 199 190
Cuts at: 4887 225
Size: 3086
Fragments arranged by size:
3086 1782 1575 659 199 190 113 97
47
EcoRI G'AATT_C
Cuts at: 502 3952 4752 502
Size: 3450 800 3498
Fragments arranged by size:
3498 3450 800
EcoRV GAT'ATC
Cuts at: 333 333
Size: 7748
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 448 5767 448
Size: 5319 2429
FspI TGC'GCA
Cuts at: 4561 5529 4561
Size: 968 6780
HaeII r_GCGC'y
Cuts at: 1332 1649 3324 4489 5182 5430 6660 7431 1332
Size: 317 1675 1165 693 248 1230 771 1649
Fragments arranged by size:
1675 1649 1230 1165 771 693 317 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 399 421 431 453 510 542 1354 1386 1487
Size: 22 10 22 57 32 812 32 101
Cuts at: 1487 1519 1734 1766 1855 1887 1929 1961 1998
Size: 32 215 32 89 32 42 32 37
Cuts at: 1998 2030 2509 2541 3071 3103 3234 3266 5590
Size: 32 479 32 530 32 131 32 2324
Cuts at: 5590 5620 5622 5652 399
Size: 30 2 30 2495
Fragments arranged by size:
2495 2324 812 530 479 215 131 101
89 57 42 37 32 32 32 32
32 32 32 32 32 32 30 30
22 22 10 2
HincII GTy'rAC
Cuts at: 1379 4787 1379
Size: 3408 4340
HindIII A'AGCT_T
Cuts at: 2118 2118
Size: 7748
KasI G'GCGC_C
Cuts at: 1645 5178 5426 1645
Size: 3533 248 3967
Fragments arranged by size:
3967 3533 248
KpnI G_GTAC'C
Cuts at: 4768 4768
Size: 7748
MluI A'CGCG_T
Cuts at: 7181 7181
Size: 7748
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 401 872 1166 4031 6507 6691 401
Size: 471 294 2865 2476 184 1458
Fragments arranged by size:
2865 2476 1458 471 294 184
MscI TGG'CCA
Cuts at: 5509 5509
Size: 7748
MslI CAynn'nnrTG
Cuts at: 1496 2835 5737 5864 1496
Size: 1339 2902 127 3380
Fragments arranged by size:
3380 2902 1339 127
MspA1I CmG'CkG
Cuts at: 1218 1535 1575 1950 2301 3869 4704 4859 5173
Size: 317 40 375 351 1568 835 155 314
Cuts at: 5173 5533 6317 6562 1218
Size: 360 784 245 2404
Fragments arranged by size:
2404 1568 835 784 375 360 351 317
314 245 155 40
NaeI GCC'GGC
Cuts at: 4675 5929 4675
Size: 1254 6494
NarI GG'CG_CC
Cuts at: 1646 5179 5427 1646
Size: 3533 248 3967
Fragments arranged by size:
3967 3533 248
NgoMIV G'CCGG_C
Cuts at: 4673 5927 4673
Size: 1254 6494
NheI G'CTAG_C
Cuts at: 3294 3294
Size: 7748
NspI r_CATG'y
Cuts at: 2095 2736 2778 4801 5301 6904 2095
Size: 641 42 2023 500 1603 2939
Fragments arranged by size:
2939 2023 1603 641 500 42
PciI A'CATG_T
Cuts at: 6900 6900
Size: 7748
PflMI CCAn_nnn'nTGG
Cuts at: 1974 3269 3550 3978 1974
Size: 1295 281 428 5744
Fragments arranged by size:
5744 1295 428 281
PfoI T'CCnGG_A
Cuts at: 215 3071 3956 215
Size: 2856 885 4007
Fragments arranged by size:
4007 2856 885
PmeI GTTT'AAAC
Cuts at: 4747 4747
Size: 7748
PmlI CAC'GTG
Cuts at: 1763 2879 1763
Size: 1116 6632
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2135 2167 6194 6226 2135
Size: 32 4027 32 3657
Fragments arranged by size:
4027 3657 32 32
PpuMI rG'GwC_Cy
Cuts at: 225 3582 4241 4697 225
Size: 3357 659 456 3276
Fragments arranged by size:
3357 3276 659 456
PspOMI G'GGCC_C
Cuts at: 4287 4401 4287
Size: 114 7634
PspXI vC'TCGA_Gb
Cuts at: 3946 3946
Size: 7748
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4264 4296 4264
Size: 32 7716
PstI C_TGCA'G
Cuts at: 320 689 740 1361 1391 1904 2021 2207 2435
Size: 369 51 621 30 513 117 186 228
Cuts at: 2435 3868 4795 5480 320
Size: 1433 927 685 2588
Fragments arranged by size:
2588 1433 927 685 621 513 369 228
186 117 51 30
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7748
PvuII CAG'CTG
Cuts at: 1218 1535 1575 2301 3869 5173 5533 1218
Size: 317 40 726 1568 1304 360 3433
Fragments arranged by size:
3433 1568 1304 726 360 317 40
RsrII CG'GwC_CG
Cuts at: 2525 4550 5943 2525
Size: 2025 1393 4330
Fragments arranged by size:
4330 2025 1393
SacI G_AGCT'C
Cuts at: 632 797 1325 2153 2522 4762 632
Size: 165 528 828 369 2240 3618
Fragments arranged by size:
3618 2240 828 528 369 165
SalI G'TCGA_C
Cuts at: 4785 4785
Size: 7748
SanDI GG'GwC_CC
Cuts at: 4241 4241
Size: 7748
SapI GCTCTTCn'nnn_
Cuts at: 1529 2165 3608 5771 5981 1529
Size: 636 1443 2163 210 3296
Fragments arranged by size:
3296 2163 1443 636 210
SbfI CC_TGCA'GG
Cuts at: 1361 1391 4795 1361
Size: 30 3404 4314
Fragments arranged by size:
4314 3404 30
ScaI AGT'ACT
Cuts at: 4063 7265 4063
Size: 3202 4546
SexAI A'CCwGG_T
Cuts at: 3470 3470
Size: 7748
SfcI C'TryA_G
Cuts at: 33 316 685 736 1345 1357 1387 1900 2017
Size: 283 369 51 609 12 30 513 117
Cuts at: 2017 2203 2431 3864 4164 4791 5476 6444 6635
Size: 186 228 1433 300 627 685 968 191
Cuts at: 6635 33
Size: 1146
Fragments arranged by size:
1433 1146 968 685 627 609 513 369
300 283 228 191 186 117 51 30
12
SfoI GGC'GCC
Cuts at: 1647 5180 5428 1647
Size: 3533 248 3967
Fragments arranged by size:
3967 3533 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7748
SmaI CCC'GGG
Cuts at: 2533 3276 4644 4770 2533
Size: 743 1368 126 5511
Fragments arranged by size:
5511 1368 743 126
SmlI C'TyrA_G
Cuts at: 386 1324 2231 2722 3946 4132 4903 5818 6532
Size: 938 907 491 1224 186 771 915 714
Cuts at: 6532 6794 386
Size: 262 1340
Fragments arranged by size:
1340 1224 938 915 907 771 714 491
262 186
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7748
SphI G_CATG'C
Cuts at: 2095 2736 4801 5301 2095
Size: 641 2065 500 4542
Fragments arranged by size:
4542 2065 641 500
SspI AAT'ATT
Cuts at: 2745 2745
Size: 7748
StuI AGG'CCT
Cuts at: 193 1843 2935 193
Size: 1650 1092 5006
Fragments arranged by size:
5006 1650 1092
StyI C'CwwG_G
Cuts at: 2975 3977 4882 5236 2975
Size: 1002 905 354 5487
Fragments arranged by size:
5487 1002 905 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1594 1849 2582 2588 3714 4455 4567 5331 5464
Size: 255 733 6 1126 741 112 764 133
Cuts at: 5464 5923 6132 1594
Size: 459 209 3210
Fragments arranged by size:
3210 1126 764 741 733 459 255 209
133 112 6
TatI w'GTAC_w
Cuts at: 1537 1662 2435 3062 3938 4061 7263 1537
Size: 125 773 627 876 123 3202 2022
Fragments arranged by size:
3202 2022 876 773 627 125 123
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4079 4111 4355 4387 7265 7297 4079
Size: 32 244 32 2878 32 4530
Fragments arranged by size:
4530 2878 244 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 5545 5545
Size: 7748
XbaI T'CTAG_A
Cuts at: 1296 2250 3438 4779 1296
Size: 954 1188 1341 4265
Fragments arranged by size:
4265 1341 1188 954
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2658 5243 2658
Size: 2585 5163
XhoI C'TCGA_G
Cuts at: 3946 3946
Size: 7748
XmaI C'CCGG_G
Cuts at: 2531 3274 4642 4768 2531
Size: 743 1368 126 5511
Fragments arranged by size:
5511 1368 743 126
XmnI GAAnn'nnTTC
Cuts at: 2309 2498 2309
Size: 189 7559
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AhdI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NheI NspI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AgeI AleI AloI AscI AvrII BaeI BcgI
BfrBI BsiWI BsmBI BssHII BstZ17I BtgI ClaI CspCI
FseI FspAI HpaI MfeI NcoI NdeI NotI NruI
NsiI PacI PasI PshAI PsiI SacII SfiI SgrAI
SnaBI SrfI SwaI ZraI