Restriction map for
pF1KA0953
(Circular) (Six-base) MAPSORT of: pF1KA0953.seq Check: 9671 from: 1 to: 5297
pF1KA0953 5297 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2199 2199
Size: 5297
AccI GT'mk_AC
Cuts at: 1865 2335 1865
Size: 470 4827
Acc65I G'GTAC_C
Cuts at: 2313 2313
Size: 5297
AclI AA'CG_TT
Cuts at: 5137 5137
Size: 5297
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1636 3122 3554 4301 4569 1636
Size: 1486 432 747 268 2364
Fragments arranged by size:
2364 1486 747 432 268
AfeI AGC'GCT
Cuts at: 1020 1288 1020
Size: 268 5029
AflIII A'CryG_T
Cuts at: 323 2649 3753 4730 323
Size: 2326 1104 977 890
Fragments arranged by size:
2326 1104 977 890
AgeI A'CCGG_T
Cuts at: 3685 3685
Size: 5297
AhdI GACnn_n'nnGTC
Cuts at: 504 504
Size: 5297
AleI CACnn'nnGTG
Cuts at: 1930 1930
Size: 5297
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1210 1242 1210
Size: 32 5265
AlwNI CAG_nnn'CTG
Cuts at: 990 1936 2602 4169 990
Size: 946 666 1567 2118
Fragments arranged by size:
2118 1567 946 666
ApaI G_GGCC'C
Cuts at: 1097 1097
Size: 5297
ApaLI G'TGCA_C
Cuts at: 4067 4067
Size: 5297
ApoI r'AATT_y
Cuts at: 593 1514 2301 4650 4906 593
Size: 921 787 2349 256 984
Fragments arranged by size:
2349 984 921 787 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5297
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5297
AvaI C'yCGr_G
Cuts at: 187 561 2002 2317 187
Size: 374 1441 315 3167
Fragments arranged by size:
3167 1441 374 315
BamHI G'GATC_C
Cuts at: 183 2322 183
Size: 2139 3158
BanI G'GyrC_C
Cuts at: 133 268 460 1192 2313 2727 2975 3010 133
Size: 135 192 732 1121 414 248 35 2420
Fragments arranged by size:
2420 1121 732 414 248 192 135 35
BanII G_rGCy'C
Cuts at: 790 1097 1258 2066 2311 3341 790
Size: 307 161 808 245 1030 2746
Fragments arranged by size:
2746 1030 808 307 245 161
BbeI G_GCGC'C
Cuts at: 272 2731 2979 272
Size: 2459 248 2590
Fragments arranged by size:
2590 2459 248
BbsI GAAGACnn'nnnn_
Cuts at: 211 643 805 1647 211
Size: 432 162 842 3861
Fragments arranged by size:
3861 842 432 162
BbvCI CC'TCA_GC
Cuts at: 1126 2147 1126
Size: 1021 4276
BciVI GTATCCnnnnn_n'
Cuts at: 1431 3195 3956 5297 1431
Size: 1764 761 1341 1431
Fragments arranged by size:
1764 1431 1341 761
BclI T'GATC_A
Cuts at: 2817 2817
Size: 5297
BglII A'GATC_T
Cuts at: 2812 4849 2812
Size: 2037 3260
BlpI GC'TnA_GC
Cuts at: 1141 2409 1141
Size: 1268 4029
Bme1580I G_kGCm'C
Cuts at: 138 1097 1881 2922 3015 4071 138
Size: 959 784 1041 93 1056 1364
Fragments arranged by size:
1364 1056 1041 959 784 93
BmgBI CAC'GTC
Cuts at: 300 1833 300
Size: 1533 3764
BmrI ACTGGGnnnn_n'
Cuts at: 2509 2590 2925 5069 2509
Size: 81 335 2144 2737
Fragments arranged by size:
2737 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 764 2217 3664 764
Size: 1453 1447 2397
Fragments arranged by size:
2397 1453 1447
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 230 448 1126 230
Size: 218 678 4401
Fragments arranged by size:
4401 678 218
Bpu10I CC'TnA_GC
Cuts at: 1126 1699 2147 1126
Size: 573 448 4276
Fragments arranged by size:
4276 573 448
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 391 2473 3352 3844 4142 391
Size: 2082 879 492 298 1546
Fragments arranged by size:
2082 1546 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 752 1963 2188 5001 752
Size: 1211 225 2813 1048
Fragments arranged by size:
2813 1211 1048 225
BsaAI yAC'GTr
Cuts at: 324 2650 324
Size: 2326 2971
BsaBI GATnn'nnATC
Cuts at: 6 1540 2162 2811 4848 6
Size: 1534 622 649 2037 455
Fragments arranged by size:
2037 1534 649 622 455
BsaHI Gr'CG_yC
Cuts at: 269 1004 2728 2976 3678 5174 269
Size: 735 1724 248 702 1496 392
Fragments arranged by size:
1724 1496 735 702 392 248
BsaWI w'CCGG_w
Cuts at: 515 2235 2486 3007 3685 3959 4106 4517 515
Size: 1720 251 521 678 274 147 411 1295
Fragments arranged by size:
1720 1295 678 521 411 274 251 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1210 1240 1273 1303 1210
Size: 30 33 30 5204
Fragments arranged by size:
5204 33 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 508 745 1231 1801 1864 2056 2193 2196 2260
Size: 237 486 570 63 192 137 3 64
Cuts at: 2260 508
Size: 3545
Fragments arranged by size:
3545 570 486 237 192 137 64 63
3
BseYI C'CCAG_C
Cuts at: 825 1673 2722 3480 4057 4553 825
Size: 848 1049 758 577 496 1569
Fragments arranged by size:
1569 1049 848 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1321 1744 1321
Size: 423 4874
BsiEI CG_ry'CG
Cuts at: 104 2839 2885 4093 104
Size: 2735 46 1208 1308
Fragments arranged by size:
2735 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1214 1639 2066 2311 3089 3279 4071 1214
Size: 425 427 245 778 190 792 2440
Fragments arranged by size:
2440 792 778 427 425 245 190
Bsp1286I G_dGCh'C
Cuts at: 138 790 1097 1214 1258 1639 1881 2066 2311
Size: 652 307 117 44 381 242 185 245
Cuts at: 2311 2922 3015 3089 3279 3341 4071 138
Size: 611 93 74 190 62 730 1364
Fragments arranged by size:
1364 730 652 611 381 307 245 242
190 185 117 93 74 62 44
BspEI T'CCGG_A
Cuts at: 2235 4517 2235
Size: 2282 3015
BspHI T'CATG_A
Cuts at: 1310 1658 4473 1310
Size: 348 2815 2134
Fragments arranged by size:
2815 2134 348
BspMI ACCTGCnnnn'nnnn_
Cuts at: 455 1407 2199 2347 2561 2863 3244 455
Size: 952 792 148 214 302 381 2508
Fragments arranged by size:
2508 952 792 381 302 214 148
BsrBI CCG'CTC
Cuts at: 50 3589 3643 5127 5292 50
Size: 3539 54 1484 165 55
Fragments arranged by size:
3539 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 329 3209 329
Size: 2880 2417
BsrFI r'CCGG_y
Cuts at: 743 1081 2123 3295 3476 3685 743
Size: 338 1042 1172 181 209 2355
Fragments arranged by size:
2355 1172 1042 338 209 181
BssHII G'CGCG_C
Cuts at: 1032 1032
Size: 5297
BssSI C'ACGA_G
Cuts at: 1213 3568 3926 1213
Size: 2355 358 2584
Fragments arranged by size:
2584 2355 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2674 2674
Size: 5297
BstBI TT'CG_AA
Cuts at: 597 3658 597
Size: 3061 2236
BstXI CCAn_nnnn'nTGG
Cuts at: 316 426 1104 2271 316
Size: 110 678 1167 3342
Fragments arranged by size:
3342 1167 678 110
BstYI r'GATC_y
Cuts at: 1 183 452 2322 2789 2812 3147 3393 4405
Size: 182 269 1870 467 23 335 246 1012
Cuts at: 4405 4491 4503 4849 1
Size: 86 12 346 449
Fragments arranged by size:
1870 1012 467 449 346 335 269 246
182 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 179 179
Size: 5297
BtgI C'CryG_G
Cuts at: 791 1064 1250 1324 791
Size: 273 186 74 4764
Fragments arranged by size:
4764 273 186 74
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1056 3247 3428 3591 1056
Size: 2191 181 163 2762
Fragments arranged by size:
2762 2191 181 163
BtsI GCAGTG_nn'
Cuts at: 369 703 2112 2540 369
Size: 334 1409 428 3126
Fragments arranged by size:
3126 1409 428 334
ClaI AT'CG_AT
Cuts at: 312 312
Size: 5297
DraI TTT'AAA
Cuts at: 933 2296 933
Size: 1363 3934
DrdI GACnn_nn'nnGTC
Cuts at: 1180 1609 3003 3861 1180
Size: 429 1394 858 2616
Fragments arranged by size:
2616 1394 858 429
EaeI y'GGCC_r
Cuts at: 416 1327 2882 3056 3447 3474 4819 416
Size: 911 1555 174 391 27 1345 894
Fragments arranged by size:
1555 1345 911 894 391 174 27
EagI C'GGCC_G
Cuts at: 2882 2882
Size: 5297
EarI CTCTTCn'nnn_
Cuts at: 394 1309 2074 3320 3530 394
Size: 915 765 1246 210 2161
Fragments arranged by size:
2161 1246 915 765 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2062 3505 3815 3961 5107 2062
Size: 1443 310 146 1146 2252
Fragments arranged by size:
2252 1443 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2064 2309 2064
Size: 245 5052
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 230 448 1126 1636 3122 3554 4301 4569 230
Size: 218 678 510 1486 432 747 268 958
Fragments arranged by size:
1486 958 747 678 510 432 268 218
EcoNI CCTnn'n_nnAGG
Cuts at: 1339 1414 1501 1339
Size: 75 87 5135
Fragments arranged by size:
5135 87 75
EcoO109I rG'GnC_Cy
Cuts at: 672 1093 1320 2436 672
Size: 421 227 1116 3533
Fragments arranged by size:
3533 1116 421 227
EcoRI G'AATT_C
Cuts at: 2301 2301
Size: 5297
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 243 3316 243
Size: 3073 2224
FspI TGC'GCA
Cuts at: 3078 3078
Size: 5297
HaeII r_GCGC'y
Cuts at: 272 1022 1290 1744 2731 2979 4001 4980 272
Size: 750 268 454 987 248 1022 979 589
Fragments arranged by size:
1022 987 979 750 589 454 268 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 490 522 730 762 1621 1653 2155 2183 2187
Size: 32 208 32 859 32 502 28 4
Cuts at: 2187 2206 2215 2217 2238 2249 3139 3169 3171
Size: 19 9 2 21 11 890 30 2
Cuts at: 3171 3201 490
Size: 30 2586
Fragments arranged by size:
2586 890 859 502 208 32 32 32
30 30 28 21 19 11 9 4
2 2
HincII GTy'rAC
Cuts at: 165 2336 165
Size: 2171 3126
KasI G'GCGC_C
Cuts at: 268 2727 2975 268
Size: 2459 248 2590
Fragments arranged by size:
2590 2459 248
KpnI G_GTAC'C
Cuts at: 2317 2317
Size: 5297
MluI A'CGCG_T
Cuts at: 4730 4730
Size: 5297
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1037 3968 4152 1037
Size: 2931 184 2182
Fragments arranged by size:
2931 2182 184
MscI TGG'CCA
Cuts at: 418 3058 418
Size: 2640 2657
MslI CAynn'nnrTG
Cuts at: 314 334 1930 1955 3286 3413 314
Size: 20 1596 25 1331 127 2198
Fragments arranged by size:
2198 1596 1331 127 25 20
MspA1I CmG'CkG
Cuts at: 354 793 1066 1134 1326 1874 2151 2408 2722
Size: 439 273 68 192 548 277 257 314
Cuts at: 2722 3082 4095 4340 354
Size: 360 1013 245 1311
Fragments arranged by size:
1311 1013 548 439 360 314 277 273
257 245 192 68
NaeI GCC'GGC
Cuts at: 3478 3478
Size: 5297
NarI GG'CG_CC
Cuts at: 269 2728 2976 269
Size: 2459 248 2590
Fragments arranged by size:
2590 2459 248
NgoMIV G'CCGG_C
Cuts at: 3476 3476
Size: 5297
NspI r_CATG'y
Cuts at: 727 1227 1998 2350 2850 3757 727
Size: 500 771 352 500 907 2267
Fragments arranged by size:
2267 907 771 500 500 352
PciI A'CATG_T
Cuts at: 3753 3753
Size: 5297
PflMI CCAn_nnn'nTGG
Cuts at: 668 1347 2289 668
Size: 679 942 3676
Fragments arranged by size:
3676 942 679
PfoI T'CCnGG_A
Cuts at: 1229 1472 1229
Size: 243 5054
PmeI GTTT'AAAC
Cuts at: 2296 2296
Size: 5297
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1210 1242 4436 4468 1210
Size: 32 3194 32 2039
Fragments arranged by size:
3194 2039 32 32
PpuMI rG'GwC_Cy
Cuts at: 672 1320 672
Size: 648 4649
PshAI GACnn'nnGTC
Cuts at: 302 302
Size: 5297
PspOMI G'GGCC_C
Cuts at: 1093 1093
Size: 5297
PspXI vC'TCGA_Gb
Cuts at: 561 561
Size: 5297
PstI C_TGCA'G
Cuts at: 452 659 1418 2210 2344 3029 452
Size: 207 759 792 134 685 2720
Fragments arranged by size:
2720 792 759 685 207 134
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5297
PvuII CAG'CTG
Cuts at: 354 1134 2151 2722 3082 354
Size: 780 1017 571 360 2569
Fragments arranged by size:
2569 1017 780 571 360
RsrII CG'GwC_CG
Cuts at: 2121 3492 2121
Size: 1371 3926
SacI G_AGCT'C
Cuts at: 2066 2311 2066
Size: 245 5052
SacII CC_GC'GG
Cuts at: 794 1067 1327 794
Size: 273 260 4764
Fragments arranged by size:
4764 273 260
SalI G'TCGA_C
Cuts at: 2334 2334
Size: 5297
SanDI GG'GwC_CC
Cuts at: 672 672
Size: 5297
SapI GCTCTTCn'nnn_
Cuts at: 3320 3530 3320
Size: 210 5087
SbfI CC_TGCA'GG
Cuts at: 1418 2210 2344 1418
Size: 792 134 4371
Fragments arranged by size:
4371 792 134
ScaI AGT'ACT
Cuts at: 1103 4814 1103
Size: 3711 1586
SexAI A'CCwGG_T
Cuts at: 971 971
Size: 5297
SfcI C'TryA_G
Cuts at: 33 448 655 718 1414 1683 2206 2340 3025
Size: 415 207 63 696 269 523 134 685
Cuts at: 3025 4018 4209 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 696 685 523 415 269 207
191 134 63
SfoI GGC'GCC
Cuts at: 270 2729 2977 270
Size: 2459 248 2590
Fragments arranged by size:
2590 2459 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5297
SmaI CCC'GGG
Cuts at: 2319 2319
Size: 5297
SmlI C'TyrA_G
Cuts at: 406 561 2452 3367 3859 4121 406
Size: 155 1891 915 492 262 1582
Fragments arranged by size:
1891 1582 915 492 262 155
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5297
SphI G_CATG'C
Cuts at: 2350 2850 2350
Size: 500 4797
StuI AGG'CCT
Cuts at: 617 617
Size: 5297
StyI C'CwwG_G
Cuts at: 908 2431 2785 908
Size: 1523 354 3420
Fragments arranged by size:
3420 1523 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2880 3013 3472 3681 2880
Size: 133 459 209 4496
Fragments arranged by size:
4496 459 209 133
TatI w'GTAC_w
Cuts at: 944 1101 4812 944
Size: 157 3711 1429
Fragments arranged by size:
3711 1429 157
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4814 4846 4814
Size: 32 5265
Tth111I GACn'n_nGTC
Cuts at: 1651 3094 1651
Size: 1443 3854
XbaI T'CTAG_A
Cuts at: 1530 2328 1530
Size: 798 4499
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1086 1497 2792 1086
Size: 411 1295 3591
Fragments arranged by size:
3591 1295 411
XhoI C'TCGA_G
Cuts at: 561 561
Size: 5297
XmaI C'CCGG_G
Cuts at: 2317 2317
Size: 5297
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AatII AflII AlfI AscI AvrII BaeI BcgI BfrBI
BglI BmtI BsiWI BsmI BsmBI BsrGI BstEII BstZ17I
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PmlI PsiI PsrI SfiI SgrAI SnaBI SrfI SspI
SwaI XmnI ZraI