Restriction map for
pF1KA0954
(Circular) (Six-base) MAPSORT of: pF1KA0954.seq Check: 2607 from: 1 to: 6566
pF1KA0954 6566 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 549 549
Size: 6566
AccI GT'mk_AC
Cuts at: 2501 3604 2501
Size: 1103 5463
Acc65I G'GTAC_C
Cuts at: 3582 3582
Size: 6566
AclI AA'CG_TT
Cuts at: 6406 6406
Size: 6566
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1420 1533 2262 2424 4391 4823 5570 5838 1420
Size: 113 729 162 1967 432 747 268 2148
Fragments arranged by size:
2148 1967 747 729 432 268 162 113
AfeI AGC'GCT
Cuts at: 142 2209 2395 142
Size: 2067 186 4313
Fragments arranged by size:
4313 2067 186
AflIII A'CryG_T
Cuts at: 1887 2732 3918 5022 5999 1887
Size: 845 1186 1104 977 2454
Fragments arranged by size:
2454 1186 1104 977 845
AgeI A'CCGG_T
Cuts at: 3419 4954 3419
Size: 1535 5031
AleI CACnn'nnGTG
Cuts at: 1525 1525
Size: 6566
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1708 3289 1708
Size: 1581 4985
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3292 3324 3292
Size: 32 6534
AlwNI CAG_nnn'CTG
Cuts at: 774 816 828 918 2713 3171 3871 5438 774
Size: 42 12 90 1795 458 700 1567 1902
Fragments arranged by size:
1902 1795 1567 700 458 90 42 12
ApaI G_GGCC'C
Cuts at: 467 1027 3060 3072 3091 3111 467
Size: 560 2033 12 19 20 3922
Fragments arranged by size:
3922 2033 560 20 19 12
ApaLI G'TGCA_C
Cuts at: 1825 1945 5336 1825
Size: 120 3391 3055
Fragments arranged by size:
3391 3055 120
ApoI r'AATT_y
Cuts at: 3177 3451 3570 5919 6175 3177
Size: 274 119 2349 256 3568
Fragments arranged by size:
3568 2349 274 256 119
AseI AT'TA_AT
Cuts at: 20 20
Size: 6566
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6566
AvaI C'yCGr_G
Cuts at: 357 384 450 531 977 1020 1366 1963 2268
Size: 27 66 81 446 43 346 597 305
Cuts at: 2268 3586 357
Size: 1318 3337
Fragments arranged by size:
3337 1318 597 446 346 305 81 66
43 27
BamHI G'GATC_C
Cuts at: 3591 3591
Size: 6566
BanI G'GyrC_C
Cuts at: 169 362 394 457 777 1073 1084 1755 2272
Size: 193 32 63 320 296 11 671 517
Cuts at: 2272 3265 3409 3582 3996 4244 4279 169
Size: 993 144 173 414 248 35 2456
Fragments arranged by size:
2456 993 671 517 414 320 296 248
193 173 144 63 35 32 11
BanII G_rGCy'C
Cuts at: 182 337 467 485 538 572 740 851 984
Size: 155 130 18 53 34 168 111 133
Cuts at: 984 1027 1349 1852 3060 3072 3091 3101 3111
Size: 43 322 503 1208 12 19 10 10
Cuts at: 3111 3580 4610 182
Size: 469 1030 2138
Fragments arranged by size:
2138 1208 1030 503 469 322 168 155
133 130 111 53 43 34 19 18
12 10 10
BbeI G_GCGC'C
Cuts at: 366 2276 4000 4248 366
Size: 1910 1724 248 2684
Fragments arranged by size:
2684 1910 1724 248
BbsI GAAGACnn'nnnn_
Cuts at: 1761 1761
Size: 6566
BbvCI CC'TCA_GC
Cuts at: 3280 3280
Size: 6566
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 958 992 958
Size: 34 6532
BciVI GTATCCnnnnn_n'
Cuts at: 4464 5225 6566 4464
Size: 761 1341 4464
Fragments arranged by size:
4464 1341 761
BclI T'GATC_A
Cuts at: 929 4086 929
Size: 3157 3409
BglI GCCn_nnn'nGGC
Cuts at: 274 996 1127 1633 1787 274
Size: 722 131 506 154 5053
Fragments arranged by size:
5053 722 506 154 131
BglII A'GATC_T
Cuts at: 1673 2036 4081 6118 1673
Size: 363 2045 2037 2121
Fragments arranged by size:
2121 2045 2037 363
BlpI GC'TnA_GC
Cuts at: 479 845 2473 3095 3678 479
Size: 366 1628 622 583 3367
Fragments arranged by size:
3367 1628 622 583 366
Bme1580I G_kGCm'C
Cuts at: 174 460 467 623 782 1027 1089 1752 1760
Size: 286 7 156 159 245 62 663 8
Cuts at: 1760 1829 1949 2590 3060 3072 3091 3111 3149
Size: 69 120 641 470 12 19 20 38
Cuts at: 3149 3412 4191 4284 5340 174
Size: 263 779 93 1056 1400
Fragments arranged by size:
1400 1056 779 663 641 470 286 263
245 159 156 120 93 69 62 38
20 19 12 8 7
BmrI ACTGGGnnnn_n'
Cuts at: 1452 3192 3778 3859 4194 6338 1452
Size: 1740 586 81 335 2144 1680
Fragments arranged by size:
2144 1740 1680 586 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 249 564 865 3088 4933 249
Size: 315 301 2223 1845 1882
Fragments arranged by size:
2223 1882 1845 315 301
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 226 248 297 635 652 1441 1615 2203
Size: 30 22 49 338 17 789 174 588
Cuts at: 2203 2224 2232 2992 3058 3193 3194 196
Size: 21 8 760 66 135 1 3568
Fragments arranged by size:
3568 789 760 588 338 174 135 66
49 30 22 21 17 8 1
Bpu10I CC'TnA_GC
Cuts at: 331 1511 2021 2868 3280 331
Size: 1180 510 847 412 3617
Fragments arranged by size:
3617 1180 847 510 412
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1360 2236 3742 4621 5113 5411 1360
Size: 876 1506 879 492 298 2515
Fragments arranged by size:
2515 1506 879 876 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1549 6270 1549
Size: 4721 1845
BsaAI yAC'GTr
Cuts at: 612 1890 2469 3919 612
Size: 1278 579 1450 3259
Fragments arranged by size:
3259 1450 1278 579
BsaBI GATnn'nnATC
Cuts at: 6 3187 4080 6117 6
Size: 3181 893 2037 455
Fragments arranged by size:
3181 2037 893 455
BsaHI Gr'CG_yC
Cuts at: 363 1283 1363 2273 3997 4245 4947 6443 363
Size: 920 80 910 1724 248 702 1496 486
Fragments arranged by size:
1724 1496 920 910 702 486 248 80
BsaWI w'CCGG_w
Cuts at: 1466 2062 2370 3419 3755 4276 4954 5228 5375
Size: 596 308 1049 336 521 678 274 147
Cuts at: 5375 5786 1466
Size: 411 2246
Fragments arranged by size:
2246 1049 678 596 521 411 336 308
274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 520 550 2057 2087 3292 3322 3368 3398 520
Size: 30 1507 30 1205 30 46 30 3688
Fragments arranged by size:
3688 1507 1205 46 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 223 2503 3294 3297 3376 223
Size: 2280 791 3 79 3413
Fragments arranged by size:
3413 2280 791 79 3
BseYI C'CCAG_C
Cuts at: 554 800 895 1338 2558 2812 2848 2854 2875
Size: 246 95 443 1220 254 36 6 21
Cuts at: 2875 3034 3480 3515 3991 4749 5326 5822 554
Size: 159 446 35 476 758 577 496 1298
Fragments arranged by size:
1298 1220 758 577 496 476 446 443
254 246 159 95 36 35 21 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 802 952 1390 1528 1912 3259 3351 802
Size: 150 438 138 384 1347 92 4017
Fragments arranged by size:
4017 1347 438 384 150 138 92
BsiEI CG_ry'CG
Cuts at: 104 1782 1807 4108 4154 5362 104
Size: 1678 25 2301 46 1208 1308
Fragments arranged by size:
2301 1678 1308 1208 46 25
BsiHKAI G_wGCw'C
Cuts at: 851 1829 1949 3210 3580 4358 4548 5340 851
Size: 978 120 1261 370 778 190 792 2077
Fragments arranged by size:
2077 1261 978 792 778 370 190 120
BsmBI CGTCTCn'nnnn_
Cuts at: 118 2179 118
Size: 2061 4505
Bsp1286I G_dGCh'C
Cuts at: 174 182 337 460 467 485 538 572 623
Size: 8 155 123 7 18 53 34 51
Cuts at: 623 740 782 851 984 1027 1089 1349 1752
Size: 117 42 69 133 43 62 260 403
Cuts at: 1752 1760 1829 1852 1949 2590 3060 3072 3091
Size: 8 69 23 97 641 470 12 19
Cuts at: 3091 3101 3111 3149 3210 3412 3580 4191 4284
Size: 10 10 38 61 202 168 611 93
Cuts at: 4284 4358 4548 4610 5340 174
Size: 74 190 62 730 1400
Fragments arranged by size:
1400 730 641 611 470 403 260 202
190 168 155 133 123 117 97 93
74 69 69 62 62 61 53 51
43 42 38 34 23 19 18 12
10 10 8 8 7
BspEI T'CCGG_A
Cuts at: 2370 5786 2370
Size: 3416 3150
BspHI T'CATG_A
Cuts at: 5742 5742
Size: 6566
BspMI ACCTGCnnnn'nnnn_
Cuts at: 549 1506 1880 3616 3830 4132 4513 549
Size: 957 374 1736 214 302 381 2602
Fragments arranged by size:
2602 1736 957 381 374 302 214
BsrBI CCG'CTC
Cuts at: 50 1014 2867 4858 4912 6396 6561 50
Size: 964 1853 1991 54 1484 165 55
Fragments arranged by size:
1991 1853 1484 964 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1046 2414 4478 1046
Size: 1368 2064 3134
Fragments arranged by size:
3134 2064 1368
BsrFI r'CCGG_y
Cuts at: 1802 2260 3419 4564 4745 4954 1802
Size: 458 1159 1145 181 209 3414
Fragments arranged by size:
3414 1159 1145 458 209 181
BsrGI T'GTAC_A
Cuts at: 722 1325 722
Size: 603 5963
BssHII G'CGCG_C
Cuts at: 1875 1875
Size: 6566
BssSI C'ACGA_G
Cuts at: 4837 5195 4837
Size: 358 6208
BstAPI GCAn_nnn'nTGC
Cuts at: 3943 3943
Size: 6566
BstBI TT'CG_AA
Cuts at: 149 4927 149
Size: 4778 1788
BstEII G'GTnAC_C
Cuts at: 2679 2679
Size: 6566
BstXI CCAn_nnnn'nTGG
Cuts at: 114 204 2566 2608 2883 114
Size: 90 2362 42 275 3797
Fragments arranged by size:
3797 2362 275 90 42
BstYI r'GATC_y
Cuts at: 1 717 1673 1925 2036 3591 4058 4081 4416
Size: 716 956 252 111 1555 467 23 335
Cuts at: 4416 4662 5674 5760 5772 6118 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1555 1012 956 716 467 449 346 335
252 246 111 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2045 3337 2045
Size: 1292 5274
BtgI C'CryG_G
Cuts at: 107 288 366 761 1776 2363 2590 2642 3048
Size: 181 78 395 1015 587 227 52 406
Cuts at: 3048 107
Size: 3625
Fragments arranged by size:
3625 1015 587 406 395 227 181 78
52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 316 971 1745 2001 4516 4697 4860 316
Size: 655 774 256 2515 181 163 2022
Fragments arranged by size:
2515 2022 774 655 256 181 163
BtsI GCAGTG_nn'
Cuts at: 508 3271 3475 3809 508
Size: 2763 204 334 3265
Fragments arranged by size:
3265 2763 334 204
DraI TTT'AAA
Cuts at: 3565 3565
Size: 6566
DraIII CAC_nnn'GTG
Cuts at: 1413 1413
Size: 6566
DrdI GACnn_nn'nnGTC
Cuts at: 4272 5130 4272
Size: 858 5708
EaeI y'GGCC_r
Cuts at: 1664 1779 1804 2225 2345 2826 2946 2981 3051
Size: 115 25 421 120 481 120 35 70
Cuts at: 3051 3424 4151 4325 4716 4743 6088 1664
Size: 373 727 174 391 27 1345 2142
Fragments arranged by size:
2142 1345 727 481 421 391 373 174
120 120 115 70 35 27 25
EagI C'GGCC_G
Cuts at: 1779 1804 4151 1779
Size: 25 2347 4194
Fragments arranged by size:
4194 2347 25
EarI CTCTTCn'nnn_
Cuts at: 865 1915 2323 4589 4799 865
Size: 1050 408 2266 210 2632
Fragments arranged by size:
2632 2266 1050 408 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 629 4774 5084 5230 6376 629
Size: 4145 310 146 1146 819
Fragments arranged by size:
4145 1146 819 310 146
EcoICRI GAG'CTC
Cuts at: 849 3578 849
Size: 2729 3837
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 226 248 297 635 652 1420 1441 1533
Size: 30 22 49 338 17 768 21 92
Cuts at: 1533 1615 2203 2224 2232 2262 2424 2992 3058
Size: 82 588 21 8 30 162 568 66
Cuts at: 3058 3193 3194 4391 4823 5570 5838 196
Size: 135 1 1197 432 747 268 924
Fragments arranged by size:
1197 924 768 747 588 568 432 338
268 162 135 92 82 66 49 30
30 22 21 21 17 8 1
EcoNI CCTnn'n_nnAGG
Cuts at: 1105 1105
Size: 6566
EcoO109I rG'GnC_Cy
Cuts at: 464 667 1024 1371 1425 2333 2493 2872 3068
Size: 203 357 347 54 908 160 379 196
Cuts at: 3068 3069 3088 3705 464
Size: 1 19 617 3325
Fragments arranged by size:
3325 908 617 379 357 347 203 196
160 54 19 1
EcoRI G'AATT_C
Cuts at: 3177 3451 3570 3177
Size: 274 119 6173
Fragments arranged by size:
6173 274 119
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1326 1497 2055 4585 1326
Size: 171 558 2530 3307
Fragments arranged by size:
3307 2530 558 171
FspI TGC'GCA
Cuts at: 583 2482 4347 583
Size: 1899 1865 2802
Fragments arranged by size:
2802 1899 1865
HaeII r_GCGC'y
Cuts at: 136 144 366 434 1819 2211 2276 2397 4000
Size: 8 222 68 1385 392 65 121 1603
Cuts at: 4000 4248 5270 6249 136
Size: 248 1022 979 453
Fragments arranged by size:
1603 1385 1022 979 453 392 248 222
121 68 65 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 520 552 2057 2089 4408 4438 4440 4470 520
Size: 32 1505 32 2319 30 2 30 2616
Fragments arranged by size:
2616 2319 1505 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3605 3605
Size: 6566
KasI G'GCGC_C
Cuts at: 362 2272 3996 4244 362
Size: 1910 1724 248 2684
Fragments arranged by size:
2684 1910 1724 248
KpnI G_GTAC'C
Cuts at: 3586 3586
Size: 6566
MfeI C'AATT_G
Cuts at: 1915 1915
Size: 6566
MluI A'CGCG_T
Cuts at: 5999 5999
Size: 6566
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 209 260 1727 1892 2649 5237 5421 209
Size: 51 1467 165 757 2588 184 1354
Fragments arranged by size:
2588 1467 1354 757 184 165 51
MscI TGG'CCA
Cuts at: 2227 2347 2828 2948 2983 3426 4327 2227
Size: 120 481 120 35 443 901 4466
Fragments arranged by size:
4466 901 481 443 120 120 35
MslI CAynn'nnrTG
Cuts at: 112 1333 1525 1618 1690 1832 2131 3166 4555
Size: 1221 192 93 72 142 299 1035 1389
Cuts at: 4555 4682 112
Size: 127 1996
Fragments arranged by size:
1996 1389 1221 1035 299 192 142 127
93 72
MspA1I CmG'CkG
Cuts at: 317 402 714 1746 3677 3991 4351 5364 5609
Size: 85 312 1032 1931 314 360 1013 245
Cuts at: 5609 317
Size: 1274
Fragments arranged by size:
1931 1274 1032 1013 360 314 312 245
85
NaeI GCC'GGC
Cuts at: 4747 4747
Size: 6566
NarI GG'CG_CC
Cuts at: 363 2273 3997 4245 363
Size: 1910 1724 248 2684
Fragments arranged by size:
2684 1910 1724 248
NcoI C'CATG_G
Cuts at: 107 366 761 2590 3048 107
Size: 259 395 1829 458 3625
Fragments arranged by size:
3625 1829 458 395 259
NgoMIV G'CCGG_C
Cuts at: 4745 4745
Size: 6566
NspI r_CATG'y
Cuts at: 1338 1575 1938 2736 3619 4119 5026 1338
Size: 237 363 798 883 500 907 2878
Fragments arranged by size:
2878 907 883 798 500 363 237
PasI CC'CwG_GG
Cuts at: 340 1005 2566 3004 3111 3405 340
Size: 665 1561 438 107 294 3501
Fragments arranged by size:
3501 1561 665 438 294 107
PciI A'CATG_T
Cuts at: 2732 5022 2732
Size: 2290 4276
PflMI CCAn_nnn'nTGG
Cuts at: 1163 2223 2921 3171 1163
Size: 1060 698 250 4558
Fragments arranged by size:
4558 1060 698 250
PfoI T'CCnGG_A
Cuts at: 203 203
Size: 6566
PmeI GTTT'AAAC
Cuts at: 3565 3565
Size: 6566
PmlI CAC'GTG
Cuts at: 612 1890 612
Size: 1278 5288
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3292 3324 5705 5737 3292
Size: 32 2381 32 4121
Fragments arranged by size:
4121 2381 32 32
PpuMI rG'GwC_Cy
Cuts at: 2493 2493
Size: 6566
PshAI GACnn'nnGTC
Cuts at: 2011 2011
Size: 6566
PspOMI G'GGCC_C
Cuts at: 463 1023 3056 3068 3087 3107 463
Size: 560 2033 12 19 20 3922
Fragments arranged by size:
3922 2033 560 20 19 12
PspXI vC'TCGA_Gb
Cuts at: 977 977
Size: 6566
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1639 1671 3226 3258 1639
Size: 32 1555 32 4947
Fragments arranged by size:
4947 1555 32 32
PstI C_TGCA'G
Cuts at: 140 713 833 1109 2405 2994 3226 3296 3499
Size: 573 120 276 1296 589 232 70 203
Cuts at: 3499 3613 4298 140
Size: 114 685 2408
Fragments arranged by size:
2408 1296 685 589 573 276 232 203
120 114 70
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6566
PvuII CAG'CTG
Cuts at: 3991 4351 3991
Size: 360 6206
RsrII CG'GwC_CG
Cuts at: 4761 4761
Size: 6566
SacI G_AGCT'C
Cuts at: 851 3580 851
Size: 2729 3837
SalI G'TCGA_C
Cuts at: 3603 3603
Size: 6566
SapI GCTCTTCn'nnn_
Cuts at: 4589 4799 4589
Size: 210 6356
SbfI CC_TGCA'GG
Cuts at: 1109 2405 3226 3613 1109
Size: 1296 821 387 4062
Fragments arranged by size:
4062 1296 821 387
ScaI AGT'ACT
Cuts at: 3202 6083 3202
Size: 2881 3685
SexAI A'CCwGG_T
Cuts at: 2495 2495
Size: 6566
SfcI C'TryA_G
Cuts at: 33 136 709 829 1105 1950 2401 2990 3222
Size: 103 573 120 276 845 451 589 232
Cuts at: 3222 3292 3495 3609 4294 5287 5478 33
Size: 70 203 114 685 993 191 1121
Fragments arranged by size:
1121 993 845 685 589 573 451 276
232 203 191 120 114 103 70
SfoI GGC'GCC
Cuts at: 364 2274 3998 4246 364
Size: 1910 1724 248 2684
Fragments arranged by size:
2684 1910 1724 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6566
SgrAI Cr'CCGG_yG
Cuts at: 3419 3419
Size: 6566
SmaI CCC'GGG
Cuts at: 359 386 452 1022 3588 359
Size: 27 66 570 2566 3337
Fragments arranged by size:
3337 2566 570 66 27
SmlI C'TyrA_G
Cuts at: 977 1375 2251 3721 4636 5128 5390 977
Size: 398 876 1470 915 492 262 2153
Fragments arranged by size:
2153 1470 915 876 492 398 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6566
SphI G_CATG'C
Cuts at: 1338 1938 3619 4119 1338
Size: 600 1681 500 3785
Fragments arranged by size:
3785 1681 600 500
StuI AGG'CCT
Cuts at: 544 1100 2380 2581 3228 544
Size: 556 1280 201 647 3882
Fragments arranged by size:
3882 1280 647 556 201
StyI C'CwwG_G
Cuts at: 107 366 485 761 1069 1880 2339 2590 2726
Size: 259 119 276 308 811 459 251 136
Cuts at: 2726 3039 3048 3700 4054 107
Size: 313 9 652 354 2619
Fragments arranged by size:
2619 811 652 459 354 313 308 276
259 251 136 119 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 814 2790 3406 3418 4149 4282 4741 4950 814
Size: 1976 616 12 731 133 459 209 2430
Fragments arranged by size:
2430 1976 731 616 459 209 133 12
TatI w'GTAC_w
Cuts at: 212 722 1325 3200 6081 212
Size: 510 603 1875 2881 697
Fragments arranged by size:
2881 1875 697 603 510
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 871 903 3368 3400 6083 6115 871
Size: 32 2465 32 2683 32 1322
Fragments arranged by size:
2683 2465 1322 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1765 2071 4363 1765
Size: 306 2292 3968
Fragments arranged by size:
3968 2292 306
XbaI T'CTAG_A
Cuts at: 3597 3597
Size: 6566
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 919 992 2418 3168 4061 114
Size: 805 73 1426 750 893 2619
Fragments arranged by size:
2619 1426 893 805 750 73
XhoI C'TCGA_G
Cuts at: 977 977
Size: 6566
XmaI C'CCGG_G
Cuts at: 357 384 450 1020 3586 357
Size: 27 66 570 2566 3337
Fragments arranged by size:
3337 2566 570 66 27
XmnI GAAnn'nnTTC
Cuts at: 1309 1309
Size: 6566
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AscI AvrII BaeI BfrBI BmgBI
BmtI BsiWI BsmI BstZ17I ClaI CspCI EcoRV FseI
FspAI HindIII HpaI NdeI NheI NotI NruI NsiI
PacI PsiI SacII SanDI SfiI SnaBI SrfI SspI
SwaI ZraI