Restriction map for
pF1KA0973
(Circular) (Six-base) MAPSORT of: pF1KA0973.seq Check: 3127 from: 1 to: 7823
pF1KA0973 7823 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1019 1019
Size: 7823
AatII G_ACGT'C
Cuts at: 4265 4265
Size: 7823
AccI GT'mk_AC
Cuts at: 650 836 1271 3065 4861 650
Size: 186 435 1794 1796 3612
Fragments arranged by size:
3612 1796 1794 435 186
Acc65I G'GTAC_C
Cuts at: 4407 4839 4407
Size: 432 7391
AclI AA'CG_TT
Cuts at: 7663 7663
Size: 7823
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1516 2223 2511 4775 5648 6080 6827 7095 1516
Size: 707 288 2264 873 432 747 268 2244
Fragments arranged by size:
2264 2244 873 747 707 432 288 268
AfeI AGC'GCT
Cuts at: 1303 1511 3022 4543 1303
Size: 208 1511 1521 4583
Fragments arranged by size:
4583 1521 1511 208
AflII C'TTAA_G
Cuts at: 3229 3229
Size: 7823
AflIII A'CryG_T
Cuts at: 5175 6279 7256 5175
Size: 1104 977 5742
Fragments arranged by size:
5742 1104 977
AgeI A'CCGG_T
Cuts at: 6211 6211
Size: 7823
AleI CACnn'nnGTG
Cuts at: 3172 3172
Size: 7823
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1322 4043 1322
Size: 2721 5102
AlwNI CAG_nnn'CTG
Cuts at: 2854 4464 5128 6695 2854
Size: 1610 664 1567 3982
Fragments arranged by size:
3982 1610 1567 664
ApaI G_GGCC'C
Cuts at: 2341 2619 2341
Size: 278 7545
ApaLI G'TGCA_C
Cuts at: 1588 4212 4661 6593 1588
Size: 2624 449 1932 2818
Fragments arranged by size:
2818 2624 1932 449
ApoI r'AATT_y
Cuts at: 985 4827 7176 7432 985
Size: 3842 2349 256 1376
Fragments arranged by size:
3842 2349 1376 256
AscI GG'CGCG_CC
Cuts at: 4070 4070
Size: 7823
AseI AT'TA_AT
Cuts at: 20 20
Size: 7823
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7823
AvaI C'yCGr_G
Cuts at: 598 791 994 1724 1774 1927 2341 3024 3480
Size: 193 203 730 50 153 414 683 456
Cuts at: 3480 3588 3967 4147 4194 4435 4519 4529 4710
Size: 108 379 180 47 241 84 10 181
Cuts at: 4710 4809 4843 598
Size: 99 34 3578
Fragments arranged by size:
3578 730 683 456 414 379 241 203
193 181 180 153 108 99 84 50
47 34 10
AvrII C'CTAG_G
Cuts at: 276 3555 276
Size: 3279 4544
BamHI G'GATC_C
Cuts at: 2599 4848 2599
Size: 2249 5574
BanI G'GyrC_C
Cuts at: 406 1255 1283 1439 2485 2644 2771 3559 4153
Size: 849 28 156 1046 159 127 788 594
Cuts at: 4153 4201 4407 4452 4506 4714 4745 4839 5253
Size: 48 206 45 54 208 31 94 414
Cuts at: 5253 5501 5536 406
Size: 248 35 2693
Fragments arranged by size:
2693 1046 849 788 594 414 248 208
206 159 156 127 94 54 48 45
35 31 28
BanII G_rGCy'C
Cuts at: 497 1676 2201 2285 2341 2416 2519 2619 2999
Size: 1179 525 84 56 75 103 100 380
Cuts at: 2999 3487 3872 4348 4628 4711 4837 5867 497
Size: 488 385 476 280 83 126 1030 2453
Fragments arranged by size:
2453 1179 1030 525 488 476 385 380
280 126 103 100 84 83 75 56
BbeI G_GCGC'C
Cuts at: 1443 2489 3563 4205 4510 5257 5505 1443
Size: 1046 1074 642 305 747 248 3761
Fragments arranged by size:
3761 1074 1046 747 642 305 248
BbsI GAAGACnn'nnnn_
Cuts at: 1063 2404 1063
Size: 1341 6482
BbvCI CC'TCA_GC
Cuts at: 592 2074 2847 4457 592
Size: 1482 773 1610 3958
Fragments arranged by size:
3958 1610 1482 773
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3402 3436 3613 3647 3402
Size: 34 177 34 7578
Fragments arranged by size:
7578 177 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5721 6482 7823 5721
Size: 761 1341 5721
Fragments arranged by size:
5721 1341 761
BclI T'GATC_A
Cuts at: 5343 5343
Size: 7823
BfrBI ATG'CAT
Cuts at: 2725 2725
Size: 7823
BglI GCCn_nnn'nGGC
Cuts at: 1091 2577 3139 4100 4336 4431 1091
Size: 1486 562 961 236 95 4483
Fragments arranged by size:
4483 1486 961 562 236 95
BglII A'GATC_T
Cuts at: 5338 7375 5338
Size: 2037 5786
BlpI GC'TnA_GC
Cuts at: 757 4342 4579 4935 757
Size: 3585 237 356 3645
Fragments arranged by size:
3645 3585 356 237
Bme1580I G_kGCm'C
Cuts at: 1592 1865 2341 2619 4216 4399 4520 4665 4717
Size: 273 476 278 1597 183 121 145 52
Cuts at: 4717 4750 4766 5448 5541 6597 1592
Size: 33 16 682 93 1056 2818
Fragments arranged by size:
2818 1597 1056 682 476 278 273 183
145 121 93 52 33 16
BmgBI CAC'GTC
Cuts at: 648 1119 648
Size: 471 7352
BmrI ACTGGGnnnn_n'
Cuts at: 413 2287 2831 4048 4678 5035 5116 5451 7595
Size: 1874 544 1217 630 357 81 335 2144
Cuts at: 7595 413
Size: 641
Fragments arranged by size:
2144 1874 1217 641 630 544 357 335
81
BmtI G_CTAG'C
Cuts at: 1558 1558
Size: 7823
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2831 4173 4604 6190 2831
Size: 1342 431 1586 4464
Fragments arranged by size:
4464 1586 1342 431
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 883 994 1036 1150 1582 2424 2479 2533 2634
Size: 111 42 114 432 842 55 54 101
Cuts at: 2634 4143 4273 883
Size: 1509 130 4433
Fragments arranged by size:
4433 1509 842 432 130 114 111 101
55 54 42
Bpu10I CC'TnA_GC
Cuts at: 592 1163 2074 2847 4442 4457 592
Size: 571 911 773 1595 15 3958
Fragments arranged by size:
3958 1595 911 773 571 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1585 2618 3874 4709 4999 5878 6370 6668 1585
Size: 1033 1256 835 290 879 492 298 2740
Fragments arranged by size:
2740 1256 1033 879 835 492 298 290
BsaI GGTCTCn'nnnn_
Cuts at: 243 380 2045 7527 243
Size: 137 1665 5482 539
Fragments arranged by size:
5482 1665 539 137
BsaAI yAC'GTr
Cuts at: 2160 3174 5176 2160
Size: 1014 2002 4807
Fragments arranged by size:
4807 2002 1014
BsaBI GATnn'nnATC
Cuts at: 6 5337 7374 6
Size: 5331 2037 455
Fragments arranged by size:
5331 2037 455
BsaHI Gr'CG_yC
Cuts at: 366 881 1440 1721 2486 3560 3619 3754 4048
Size: 515 559 281 765 1074 59 135 294
Cuts at: 4048 4202 4262 4507 5254 5502 6204 7700 366
Size: 154 60 245 747 248 702 1496 489
Fragments arranged by size:
1496 1074 765 747 702 559 515 489
294 281 248 245 154 135 60 59
BsaWI w'CCGG_w
Cuts at: 2418 3392 5012 5533 6211 6485 6632 7043 2418
Size: 974 1620 521 678 274 147 411 3198
Fragments arranged by size:
3198 1620 974 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 490 520 2149 2179 2254 2284 490
Size: 30 1629 30 75 30 6029
Fragments arranged by size:
6029 1629 75 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 336 435 603 703 1012 1900 1954 2206 2209
Size: 99 168 100 309 888 54 252 3
Cuts at: 2209 2359 2641 2949 3877 4579 336
Size: 150 282 308 928 702 3580
Fragments arranged by size:
3580 928 888 702 309 308 282 252
168 150 100 99 54 3
BseYI C'CCAG_C
Cuts at: 564 1089 2373 2518 2581 2965 3129 3154 3582
Size: 525 1284 145 63 384 164 25 428
Cuts at: 3582 3637 3915 4544 4647 4666 4767 5248 6006
Size: 55 278 629 103 19 101 481 758
Cuts at: 6006 6583 7079 564
Size: 577 496 1308
Fragments arranged by size:
1308 1284 758 629 577 525 496 481
428 384 278 164 145 103 101 63
55 25 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 3631 3808 3919 4254 4309 4372 4677 4694 3631
Size: 177 111 335 55 63 305 17 6760
Fragments arranged by size:
6760 335 305 177 111 63 55 17
BsiEI CG_ry'CG
Cuts at: 104 1025 3704 3908 5365 5411 6619 104
Size: 921 2679 204 1457 46 1208 1308
Fragments arranged by size:
2679 1457 1308 1208 921 204 46
BsiHKAI G_wGCw'C
Cuts at: 497 526 758 1592 2332 3595 4216 4665 4837
Size: 29 232 834 740 1263 621 449 172
Cuts at: 4837 5615 5805 6597 497
Size: 778 190 792 1723
Fragments arranged by size:
1723 1263 834 792 778 740 621 449
232 190 172 29
BsmBI CGTCTCn'nnnn_
Cuts at: 435 1540 4039 435
Size: 1105 2499 4219
Fragments arranged by size:
4219 2499 1105
Bsp1286I G_dGCh'C
Cuts at: 497 526 758 1592 1676 1865 2201 2285 2332
Size: 29 232 834 84 189 336 84 47
Cuts at: 2332 2341 2416 2519 2619 2999 3487 3595 3872
Size: 9 75 103 100 380 488 108 277
Cuts at: 3872 4216 4348 4399 4520 4628 4665 4711 4717
Size: 344 132 51 121 108 37 46 6
Cuts at: 4717 4750 4766 4837 5448 5541 5615 5805 5867
Size: 33 16 71 611 93 74 190 62
Cuts at: 5867 6597 497
Size: 730 1723
Fragments arranged by size:
1723 834 730 611 488 380 344 336
277 232 190 189 132 121 108 108
103 100 93 84 84 75 74 71
62 51 47 46 37 33 29 16
9 6
BspEI T'CCGG_A
Cuts at: 2418 3392 7043 2418
Size: 974 3651 3198
Fragments arranged by size:
3651 3198 974
BspHI T'CATG_A
Cuts at: 1676 2867 6999 1676
Size: 1191 4132 2500
Fragments arranged by size:
4132 2500 1191
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1019 3425 4071 4873 5087 5389 5770 1019
Size: 2406 646 802 214 302 381 3072
Fragments arranged by size:
3072 2406 802 646 381 302 214
BsrBI CCG'CTC
Cuts at: 50 581 2141 3002 3434 3455 3533 3683 3885
Size: 531 1560 861 432 21 78 150 202
Cuts at: 3885 4257 4583 6115 6169 7653 7818 50
Size: 372 326 1532 54 1484 165 55
Fragments arranged by size:
1560 1532 1484 861 531 432 372 326
202 165 150 78 55 54 21
BsrDI GCAATG_nn'
Cuts at: 5735 5735
Size: 7823
BsrFI r'CCGG_y
Cuts at: 368 2356 3504 3703 4370 5821 6002 6211 368
Size: 1988 1148 199 667 1451 181 209 1980
Fragments arranged by size:
1988 1980 1451 1148 667 209 199 181
BssHII G'CGCG_C
Cuts at: 3508 3947 4070 3508
Size: 439 123 7261
Fragments arranged by size:
7261 439 123
BssSI C'ACGA_G
Cuts at: 2278 4360 6094 6452 2278
Size: 2082 1734 358 3649
Fragments arranged by size:
3649 2082 1734 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1421 3501 5200 1421
Size: 2080 1699 4044
Fragments arranged by size:
4044 2080 1699
BstBI TT'CG_AA
Cuts at: 3269 6184 3269
Size: 2915 4908
BstEII G'GTnAC_C
Cuts at: 271 2692 271
Size: 2421 5402
BstXI CCAn_nnnn'nTGG
Cuts at: 1814 1995 2457 2751 3178 4326 1814
Size: 181 462 294 427 1148 5311
Fragments arranged by size:
5311 1148 462 427 294 181
BstYI r'GATC_y
Cuts at: 1 1352 2599 4848 5315 5338 5673 5919 6931
Size: 1351 1247 2249 467 23 335 246 1012
Cuts at: 6931 7017 7029 7375 1
Size: 86 12 346 449
Fragments arranged by size:
2249 1351 1247 1012 467 449 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 3209 3209
Size: 7823
BtgI C'CryG_G
Cuts at: 482 830 1628 2201 2594 4002 482
Size: 348 798 573 393 1408 4303
Fragments arranged by size:
4303 1408 798 573 393 348
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1752 3655 3705 3815 5773 5954 6117 1752
Size: 1903 50 110 1958 181 163 3458
Fragments arranged by size:
3458 1958 1903 181 163 110 50
BtsI GCAGTG_nn'
Cuts at: 674 2852 2885 3046 3257 3766 4665 4677 4682
Size: 2178 33 161 211 509 899 12 5
Cuts at: 4682 5066 674
Size: 384 3431
Fragments arranged by size:
3431 2178 899 509 384 211 161 33
12 5
DraI TTT'AAA
Cuts at: 4822 4822
Size: 7823
DraIII CAC_nnn'GTG
Cuts at: 1053 2160 3052 3783 1053
Size: 1107 892 731 5093
Fragments arranged by size:
5093 1107 892 731
DrdI GACnn_nn'nnGTC
Cuts at: 3082 5529 6387 3082
Size: 2447 858 4518
Fragments arranged by size:
4518 2447 858
EaeI y'GGCC_r
Cuts at: 552 743 1022 2354 3701 3815 3905 4242 4316
Size: 191 279 1332 1347 114 90 337 74
Cuts at: 4316 4328 5408 5582 5973 6000 7345 552
Size: 12 1080 174 391 27 1345 1030
Fragments arranged by size:
1347 1345 1332 1080 1030 391 337 279
191 174 114 90 74 27 12
EagI C'GGCC_G
Cuts at: 1022 3701 3905 5408 1022
Size: 2679 204 1503 3437
Fragments arranged by size:
3437 2679 1503 204
EarI CTCTTCn'nnn_
Cuts at: 2226 2800 3394 5846 6056 2226
Size: 574 594 2452 210 3993
Fragments arranged by size:
3993 2452 594 574 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 166 373 1072 2200 3339 3537 4081 6031 6341
Size: 207 699 1128 1139 198 544 1950 310
Cuts at: 6341 6487 7633 166
Size: 146 1146 356
Fragments arranged by size:
1950 1146 1139 1128 699 544 356 310
207 198 146
EcoICRI GAG'CTC
Cuts at: 495 4835 495
Size: 4340 3483
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 883 994 1036 1150 1516 1582 2223 2424 2479
Size: 111 42 114 366 66 641 201 55
Cuts at: 2479 2511 2533 2634 4143 4273 4775 5648 6080
Size: 32 22 101 1509 130 502 873 432
Cuts at: 6080 6827 7095 883
Size: 747 268 1611
Fragments arranged by size:
1611 1509 873 747 641 502 432 366
268 201 130 114 111 101 66 55
42 32 22
EcoNI CCTnn'n_nnAGG
Cuts at: 351 1015 351
Size: 664 7159
EcoO109I rG'GnC_Cy
Cuts at: 1754 1941 2337 2338 2616 3159 4062 4524 4962
Size: 187 396 1 278 543 903 462 438
Cuts at: 4962 1754
Size: 4615
Fragments arranged by size:
4615 903 543 462 438 396 278 187
1
EcoRI G'AATT_C
Cuts at: 985 4827 985
Size: 3842 3981
EcoRV GAT'ATC
Cuts at: 1380 1380
Size: 7823
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1350 3057 5842 1350
Size: 1707 2785 3331
Fragments arranged by size:
3331 2785 1707
FspI TGC'GCA
Cuts at: 680 2566 3955 5604 680
Size: 1886 1389 1649 2899
Fragments arranged by size:
2899 1886 1649 1389
HaeII r_GCGC'y
Cuts at: 459 606 1268 1305 1443 1513 2021 2489 2509
Size: 147 662 37 138 70 508 468 20
Cuts at: 2509 2534 2657 3024 3563 4018 4205 4342 4510
Size: 25 123 367 539 455 187 137 168
Cuts at: 4510 4545 5257 5505 6527 7506 459
Size: 35 712 248 1022 979 776
Fragments arranged by size:
1022 979 776 712 662 539 508 468
455 367 248 187 168 147 138 137
123 70 37 35 25 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 627 659 1162 1194 1889 1921 1977 2009 2125
Size: 32 503 32 695 32 56 32 116
Cuts at: 2125 2157 2254 2286 4174 4206 5665 5695 5697
Size: 32 97 32 1888 32 1459 30 2
Cuts at: 5697 5727 627
Size: 30 2723
Fragments arranged by size:
2723 1888 1459 695 503 116 97 56
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 4862 4862
Size: 7823
KasI G'GCGC_C
Cuts at: 1439 2485 3559 4201 4506 5253 5501 1439
Size: 1046 1074 642 305 747 248 3761
Fragments arranged by size:
3761 1074 1046 747 642 305 248
KpnI G_GTAC'C
Cuts at: 4411 4843 4411
Size: 432 7391
MluI A'CGCG_T
Cuts at: 7256 7256
Size: 7823
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1178 2090 2612 2963 4061 4532 6494 6678 1178
Size: 912 522 351 1098 471 1962 184 2323
Fragments arranged by size:
2323 1962 1098 912 522 471 351 184
MscI TGG'CCA
Cuts at: 4318 4330 5584 4318
Size: 12 1254 6557
Fragments arranged by size:
6557 1254 12
MslI CAynn'nnrTG
Cuts at: 1456 1577 1627 3172 3176 5812 5939 1456
Size: 121 50 1545 4 2636 127 3340
Fragments arranged by size:
3340 2636 1545 127 121 50 4
MspA1I CmG'CkG
Cuts at: 264 468 927 1089 2268 2302 2596 2986 3726
Size: 204 459 162 1179 34 294 390 740
Cuts at: 3726 4934 5248 5608 6621 6866 264
Size: 1208 314 360 1013 245 1221
Fragments arranged by size:
1221 1208 1179 1013 740 459 390 360
314 294 245 204 162 34
NaeI GCC'GGC
Cuts at: 2358 3506 3705 4372 6004 2358
Size: 1148 199 667 1632 4177
Fragments arranged by size:
4177 1632 1148 667 199
NarI GG'CG_CC
Cuts at: 1440 2486 3560 4202 4507 5254 5502 1440
Size: 1046 1074 642 305 747 248 3761
Fragments arranged by size:
3761 1074 1046 747 642 305 248
NcoI C'CATG_G
Cuts at: 1628 4002 1628
Size: 2374 5449
NgoMIV G'CCGG_C
Cuts at: 2356 3504 3703 4370 6002 2356
Size: 1148 199 667 1632 4177
Fragments arranged by size:
4177 1632 1148 667 199
NheI G'CTAG_C
Cuts at: 1554 1554
Size: 7823
NsiI A_TGCA'T
Cuts at: 2727 2727
Size: 7823
NspI r_CATG'y
Cuts at: 1419 1536 3115 4876 5376 6283 1419
Size: 117 1579 1761 500 907 2959
Fragments arranged by size:
2959 1761 1579 907 500 117
PciI A'CATG_T
Cuts at: 6279 6279
Size: 7823
PflMI CCAn_nnn'nTGG
Cuts at: 1014 3783 1014
Size: 2769 5054
PfoI T'CCnGG_A
Cuts at: 1733 2456 1733
Size: 723 7100
PmeI GTTT'AAAC
Cuts at: 4822 4822
Size: 7823
PmlI CAC'GTG
Cuts at: 2160 3174 2160
Size: 1014 6809
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6962 6994 6962
Size: 32 7791
PpuMI rG'GwC_Cy
Cuts at: 1754 3159 1754
Size: 1405 6418
PshAI GACnn'nnGTC
Cuts at: 4110 4684 4110
Size: 574 7249
PspOMI G'GGCC_C
Cuts at: 2337 2615 2337
Size: 278 7545
PspXI vC'TCGA_Gb
Cuts at: 3588 3967 3588
Size: 379 7444
PstI C_TGCA'G
Cuts at: 1985 2307 2880 4870 5555 1985
Size: 322 573 1990 685 4253
Fragments arranged by size:
4253 1990 685 573 322
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7823
PvuII CAG'CTG
Cuts at: 468 927 1089 2268 2302 2986 5248 5608 468
Size: 459 162 1179 34 684 2262 360 2683
Fragments arranged by size:
2683 2262 1179 684 459 360 162 34
RsrII CG'GwC_CG
Cuts at: 6018 6018
Size: 7823
SacI G_AGCT'C
Cuts at: 497 4837 497
Size: 4340 3483
SacII CC_GC'GG
Cuts at: 2597 2597
Size: 7823
SalI G'TCGA_C
Cuts at: 4860 4860
Size: 7823
SanDI GG'GwC_CC
Cuts at: 1754 1754
Size: 7823
SapI GCTCTTCn'nnn_
Cuts at: 5846 6056 5846
Size: 210 7613
SbfI CC_TGCA'GG
Cuts at: 4870 4870
Size: 7823
ScaI AGT'ACT
Cuts at: 7340 7340
Size: 7823
SexAI A'CCwGG_T
Cuts at: 1274 1274
Size: 7823
SfcI C'TryA_G
Cuts at: 33 1981 2303 2876 3087 4866 5551 6544 6735
Size: 1948 322 573 211 1779 685 993 191
Cuts at: 6735 33
Size: 1121
Fragments arranged by size:
1948 1779 1121 993 685 573 322 211
191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2577 2577
Size: 7823
SfoI GGC'GCC
Cuts at: 1441 2487 3561 4203 4508 5255 5503 1441
Size: 1046 1074 642 305 747 248 3761
Fragments arranged by size:
3761 1074 1046 747 642 305 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7823
SgrAI Cr'CCGG_yG
Cuts at: 368 368
Size: 7823
SmaI CCC'GGG
Cuts at: 600 793 3482 4196 4437 4531 4811 4845 600
Size: 193 2689 714 241 94 280 34 3578
Fragments arranged by size:
3578 2689 714 280 241 193 94 34
SmlI C'TyrA_G
Cuts at: 1600 2633 3229 3588 3889 3967 4724 4978 5893
Size: 1033 596 359 301 78 757 254 915
Cuts at: 5893 6385 6647 1600
Size: 492 262 2776
Fragments arranged by size:
2776 1033 915 757 596 492 359 301
262 254 78
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7823
SphI G_CATG'C
Cuts at: 4876 5376 4876
Size: 500 7323
SspI AAT'ATT
Cuts at: 1503 1503
Size: 7823
StuI AGG'CCT
Cuts at: 1363 2571 2811 4775 1363
Size: 1208 240 1964 4411
Fragments arranged by size:
4411 1964 1208 240
StyI C'CwwG_G
Cuts at: 276 1037 1628 2333 2734 3353 3555 4002 4065
Size: 761 591 705 401 619 202 447 63
Cuts at: 4065 4331 4478 4957 5311 276
Size: 266 147 479 354 2788
Fragments arranged by size:
2788 761 705 619 591 479 447 401
354 266 202 147 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 252 2979 3061 3184 4536 4680 5406 5539 5998
Size: 2727 82 123 1352 144 726 133 459
Cuts at: 5998 6207 252
Size: 209 1868
Fragments arranged by size:
2727 1868 1352 726 459 209 144 133
123 82
TatI w'GTAC_w
Cuts at: 1535 1763 7338 1535
Size: 228 5575 2020
Fragments arranged by size:
5575 2020 228
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 490 522 2149 2181 2664 2696 3724 3756 7340
Size: 32 1627 32 483 32 1028 32 3584
Cuts at: 7340 7372 490
Size: 32 941
Fragments arranged by size:
3584 1627 1028 941 483 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 807 5620 807
Size: 4813 3010
XbaI T'CTAG_A
Cuts at: 4854 4854
Size: 7823
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 681 1814 2478 4725 5318 681
Size: 1133 664 2247 593 3186
Fragments arranged by size:
3186 2247 1133 664 593
XhoI C'TCGA_G
Cuts at: 3588 3967 3588
Size: 379 7444
XmaI C'CCGG_G
Cuts at: 598 791 3480 4194 4435 4529 4809 4843 598
Size: 193 2689 714 241 94 280 34 3578
Fragments arranged by size:
3578 2689 714 280 241 193 94 34
XmnI GAAnn'nnTTC
Cuts at: 1225 1225
Size: 7823
ZraI GAC'GTC
Cuts at: 4263 4263
Size: 7823
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NsiI NspI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AhdI AloI BaeI BsiWI BsmI BsrGI BstZ17I ClaI
CspCI FseI FspAI HindIII HpaI MfeI NdeI NotI
NruI PacI PasI PsiI PsrI SnaBI SrfI SwaI