Restriction map for
pF1KA0982
(Circular) (Six-base) MAPSORT of: pF1KA0982.seq Check: 1929 from: 1 to: 4595
pF1KA0982 4595 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AatII G_ACGT'C
Cuts at: 1045 1045
Size: 4595
AccI GT'mk_AC
Cuts at: 1633 1633
Size: 4595
Acc65I G'GTAC_C
Cuts at: 1611 1611
Size: 4595
AclI AA'CG_TT
Cuts at: 372 4435 372
Size: 4063 532
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 404 493 2420 2852 3599 3867 404
Size: 89 1927 432 747 268 1132
Fragments arranged by size:
1927 1132 747 432 268 89
AflIII A'CryG_T
Cuts at: 230 1947 3051 4028 230
Size: 1717 1104 977 797
Fragments arranged by size:
1717 1104 977 797
AgeI A'CCGG_T
Cuts at: 2983 2983
Size: 4595
AlwNI CAG_nnn'CTG
Cuts at: 471 1900 3467 471
Size: 1429 1567 1599
Fragments arranged by size:
1599 1567 1429
ApaLI G'TGCA_C
Cuts at: 3365 3365
Size: 4595
ApoI r'AATT_y
Cuts at: 310 714 724 1599 3948 4204 310
Size: 404 10 875 2349 256 701
Fragments arranged by size:
2349 875 701 404 256 10
AseI AT'TA_AT
Cuts at: 20 20
Size: 4595
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4595
AvaI C'yCGr_G
Cuts at: 622 1615 622
Size: 993 3602
BamHI G'GATC_C
Cuts at: 1620 1620
Size: 4595
BanI G'GyrC_C
Cuts at: 1611 2025 2273 2308 1611
Size: 414 248 35 3898
Fragments arranged by size:
3898 414 248 35
BanII G_rGCy'C
Cuts at: 709 878 1052 1609 2639 709
Size: 169 174 557 1030 2665
Fragments arranged by size:
2665 1030 557 174 169
BbeI G_GCGC'C
Cuts at: 2029 2277 2029
Size: 248 4347
BbsI GAAGACnn'nnnn_
Cuts at: 481 1505 481
Size: 1024 3571
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 113 124 147 90
Size: 23 11 23 4538
Fragments arranged by size:
4538 23 23 11
BciVI GTATCCnnnnn_n'
Cuts at: 2493 3254 4595 2493
Size: 761 1341 2493
Fragments arranged by size:
2493 1341 761
BclI T'GATC_A
Cuts at: 2115 2115
Size: 4595
BglI GCCn_nnn'nGGC
Cuts at: 704 950 1460 704
Size: 246 510 3839
Fragments arranged by size:
3839 510 246
BglII A'GATC_T
Cuts at: 2110 4147 2110
Size: 2037 2558
BlpI GC'TnA_GC
Cuts at: 1707 1707
Size: 4595
Bme1580I G_kGCm'C
Cuts at: 1073 2220 2313 3369 1073
Size: 1147 93 1056 2299
Fragments arranged by size:
2299 1147 1056 93
BmgBI CAC'GTC
Cuts at: 1035 1035
Size: 4595
BmrI ACTGGGnnnn_n'
Cuts at: 1807 1888 2223 4367 1807
Size: 81 335 2144 2035
Fragments arranged by size:
2144 2035 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2962 2962
Size: 4595
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 495 782 804 495
Size: 287 22 4286
Fragments arranged by size:
4286 287 22
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 922 1771 2650 3142 3440 922
Size: 849 879 492 298 2077
Fragments arranged by size:
2077 879 849 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4299 4299
Size: 4595
BsaAI yAC'GTr
Cuts at: 346 702 1948 346
Size: 356 1246 2993
Fragments arranged by size:
2993 1246 356
BsaBI GATnn'nnATC
Cuts at: 6 2109 4146 6
Size: 2103 2037 455
Fragments arranged by size:
2103 2037 455
BsaHI Gr'CG_yC
Cuts at: 1042 2026 2274 2976 4472 1042
Size: 984 248 702 1496 1165
Fragments arranged by size:
1496 1165 984 702 248
BsaWI w'CCGG_w
Cuts at: 1013 1784 2305 2983 3257 3404 3815 1013
Size: 771 521 678 274 147 411 1793
Fragments arranged by size:
1793 771 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1232 1262 1232
Size: 30 4565
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1119 1119
Size: 4595
BseYI C'CCAG_C
Cuts at: 812 1109 2020 2778 3355 3851 812
Size: 297 911 758 577 496 1556
Fragments arranged by size:
1556 911 758 577 496 297
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 817 1080 817
Size: 263 4332
BsiEI CG_ry'CG
Cuts at: 104 2137 2183 3391 104
Size: 2033 46 1208 1308
Fragments arranged by size:
2033 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 623 862 1052 1609 2387 2577 3369 623
Size: 239 190 557 778 190 792 1849
Fragments arranged by size:
1849 792 778 557 239 190 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1033 1033
Size: 4595
Bsp1286I G_dGCh'C
Cuts at: 623 709 862 878 1052 1073 1609 2220 2313
Size: 86 153 16 174 21 536 611 93
Cuts at: 2313 2387 2577 2639 3369 623
Size: 74 190 62 730 1849
Fragments arranged by size:
1849 730 611 536 190 174 153 93
86 74 62 21 16
BspEI T'CCGG_A
Cuts at: 3815 3815
Size: 4595
BspHI T'CATG_A
Cuts at: 3771 3771
Size: 4595
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1645 1859 2161 2542 1645
Size: 214 302 381 3698
Fragments arranged by size:
3698 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2887 2941 4425 4590 50
Size: 2837 54 1484 165 55
Fragments arranged by size:
2837 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1310 1573 2507 1310
Size: 263 934 3398
Fragments arranged by size:
3398 934 263
BsrFI r'CCGG_y
Cuts at: 1538 2593 2774 2983 1538
Size: 1055 181 209 3150
Fragments arranged by size:
3150 1055 209 181
BssSI C'ACGA_G
Cuts at: 550 1117 2866 3224 550
Size: 567 1749 358 1921
Fragments arranged by size:
1921 1749 567 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1972 1972
Size: 4595
BstBI TT'CG_AA
Cuts at: 2956 2956
Size: 4595
BstEII G'GTnAC_C
Cuts at: 1122 1122
Size: 4595
BstYI r'GATC_y
Cuts at: 1 1303 1620 2087 2110 2445 2691 3703 3789
Size: 1302 317 467 23 335 246 1012 86
Cuts at: 3789 3801 4147 1
Size: 12 346 449
Fragments arranged by size:
1302 1012 467 449 346 335 317 246
86 23 12
BtgI C'CryG_G
Cuts at: 1235 1235
Size: 4595
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 945 1277 2545 2726 2889 945
Size: 332 1268 181 163 2651
Fragments arranged by size:
2651 1268 332 181 163
BtsI GCAGTG_nn'
Cuts at: 752 1089 1838 752
Size: 337 749 3509
Fragments arranged by size:
3509 749 337
DraI TTT'AAA
Cuts at: 1594 1594
Size: 4595
DraIII CAC_nnn'GTG
Cuts at: 1185 1509 1185
Size: 324 4271
DrdI GACnn_nn'nnGTC
Cuts at: 2301 3159 2301
Size: 858 3737
EaeI y'GGCC_r
Cuts at: 567 599 1362 2180 2354 2745 2772 4117 567
Size: 32 763 818 174 391 27 1345 1045
Fragments arranged by size:
1345 1045 818 763 391 174 32 27
EagI C'GGCC_G
Cuts at: 2180 2180
Size: 4595
EarI CTCTTCn'nnn_
Cuts at: 2618 2828 2618
Size: 210 4385
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1138 2803 3113 3259 4405 1138
Size: 1665 310 146 1146 1328
Fragments arranged by size:
1665 1328 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1050 1607 1050
Size: 557 4038
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 404 493 495 782 804 2420 2852 3599 3867
Size: 89 2 287 22 1616 432 747 268
Cuts at: 3867 404
Size: 1132
Fragments arranged by size:
1616 1132 747 432 287 268 89 22
2
EcoO109I rG'GnC_Cy
Cuts at: 1167 1734 1167
Size: 567 4028
EcoRI G'AATT_C
Cuts at: 1599 1599
Size: 4595
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2614 2614
Size: 4595
FspI TGC'GCA
Cuts at: 2376 2376
Size: 4595
HaeII r_GCGC'y
Cuts at: 2029 2277 3299 4278 2029
Size: 248 1022 979 2346
Fragments arranged by size:
2346 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2437 2467 2469 2499 2437
Size: 30 2 30 4533
Fragments arranged by size:
4533 30 30 2
HincII GTy'rAC
Cuts at: 1085 1634 1085
Size: 549 4046
KasI G'GCGC_C
Cuts at: 2025 2273 2025
Size: 248 4347
KpnI G_GTAC'C
Cuts at: 1615 1615
Size: 4595
MluI A'CGCG_T
Cuts at: 4028 4028
Size: 4595
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 992 1534 3266 3450 992
Size: 542 1732 184 2137
Fragments arranged by size:
2137 1732 542 184
MscI TGG'CCA
Cuts at: 601 2356 601
Size: 1755 2840
MslI CAynn'nnrTG
Cuts at: 2584 2711 2584
Size: 127 4468
MspA1I CmG'CkG
Cuts at: 801 1113 1706 2020 2380 3393 3638 801
Size: 312 593 314 360 1013 245 1758
Fragments arranged by size:
1758 1013 593 360 314 312 245
NaeI GCC'GGC
Cuts at: 2776 2776
Size: 4595
NarI GG'CG_CC
Cuts at: 2026 2274 2026
Size: 248 4347
NgoMIV G'CCGG_C
Cuts at: 2774 2774
Size: 4595
NspI r_CATG'y
Cuts at: 234 1648 2148 3055 234
Size: 1414 500 907 1774
Fragments arranged by size:
1774 1414 907 500
PciI A'CATG_T
Cuts at: 230 3051 230
Size: 2821 1774
PflMI CCAn_nnn'nTGG
Cuts at: 954 1553 954
Size: 599 3996
PmeI GTTT'AAAC
Cuts at: 1594 1594
Size: 4595
PmlI CAC'GTG
Cuts at: 702 702
Size: 4595
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3734 3766 3734
Size: 32 4563
PpuMI rG'GwC_Cy
Cuts at: 1167 1167
Size: 4595
PstI C_TGCA'G
Cuts at: 1642 2327 1642
Size: 685 3910
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4595
PvuII CAG'CTG
Cuts at: 801 2020 2380 801
Size: 1219 360 3016
Fragments arranged by size:
3016 1219 360
RsrII CG'GwC_CG
Cuts at: 2790 2790
Size: 4595
SacI G_AGCT'C
Cuts at: 1052 1609 1052
Size: 557 4038
SalI G'TCGA_C
Cuts at: 1632 1632
Size: 4595
SanDI GG'GwC_CC
Cuts at: 1167 1167
Size: 4595
SapI GCTCTTCn'nnn_
Cuts at: 2618 2828 2618
Size: 210 4385
SbfI CC_TGCA'GG
Cuts at: 1642 1642
Size: 4595
ScaI AGT'ACT
Cuts at: 4112 4112
Size: 4595
SfcI C'TryA_G
Cuts at: 33 1638 2323 3316 3507 33
Size: 1605 685 993 191 1121
Fragments arranged by size:
1605 1121 993 685 191
SfoI GGC'GCC
Cuts at: 2027 2275 2027
Size: 248 4347
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4595
SmaI CCC'GGG
Cuts at: 1617 1617
Size: 4595
SmlI C'TyrA_G
Cuts at: 937 1750 2665 3157 3419 937
Size: 813 915 492 262 2113
Fragments arranged by size:
2113 915 813 492 262
SnaBI TAC'GTA
Cuts at: 346 346
Size: 4595
SpeI A'CTAG_T
Cuts at: 67 829 67
Size: 762 3833
SphI G_CATG'C
Cuts at: 1648 2148 1648
Size: 500 4095
StyI C'CwwG_G
Cuts at: 1729 2083 1729
Size: 354 4241
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1123 2178 2311 2770 2979 1123
Size: 1055 133 459 209 2739
Fragments arranged by size:
2739 1055 459 209 133
TatI w'GTAC_w
Cuts at: 274 560 4110 274
Size: 286 3550 759
Fragments arranged by size:
3550 759 286
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1232 1264 4112 4144 1232
Size: 32 2848 32 1683
Fragments arranged by size:
2848 1683 32 32
Tth111I GACn'n_nGTC
Cuts at: 2392 2392
Size: 4595
XbaI T'CTAG_A
Cuts at: 1626 1626
Size: 4595
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2090 2090
Size: 4595
XmaI C'CCGG_G
Cuts at: 1615 1615
Size: 4595
XmnI GAAnn'nnTTC
Cuts at: 1192 1192
Size: 4595
ZraI GAC'GTC
Cuts at: 1043 1043
Size: 4595
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PflMI PmeI PmlI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BfrBI BmtI Bpu10I BsiWI
BsmI BsrGI BssHII BstXI BstZ17I Bsu36I ClaI CspCI
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PfoI
PshAI PsiI PspOMI PspXI PsrI SacII SexAI SfiI
SgrAI SrfI SspI StuI SwaI XhoI