Restriction map for
pF1KA0985
(Circular) (Six-base) MAPSORT of: pF1KA0985.seq Check: 6534 from: 1 to: 5195
pF1KA0985 5195 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1719 1719
Size: 5195
AccI GT'mk_AC
Cuts at: 2233 2233
Size: 5195
Acc65I G'GTAC_C
Cuts at: 2211 2211
Size: 5195
AclI AA'CG_TT
Cuts at: 5035 5035
Size: 5195
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1384 1639 3020 3452 4199 4467 1384
Size: 255 1381 432 747 268 2112
Fragments arranged by size:
2112 1381 747 432 268 255
AfeI AGC'GCT
Cuts at: 2098 2149 2098
Size: 51 5144
AflIII A'CryG_T
Cuts at: 2177 2547 3651 4628 2177
Size: 370 1104 977 2744
Fragments arranged by size:
2744 1104 977 370
AgeI A'CCGG_T
Cuts at: 3583 3583
Size: 5195
AleI CACnn'nnGTG
Cuts at: 218 218
Size: 5195
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1856 1856
Size: 5195
AlwNI CAG_nnn'CTG
Cuts at: 760 772 2500 4067 760
Size: 12 1728 1567 1888
Fragments arranged by size:
1888 1728 1567 12
ApaI G_GGCC'C
Cuts at: 742 742
Size: 5195
ApaLI G'TGCA_C
Cuts at: 1338 1831 3965 1338
Size: 493 2134 2568
Fragments arranged by size:
2568 2134 493
ApoI r'AATT_y
Cuts at: 1297 1567 1582 1638 1954 2199 4548 4804 1297
Size: 270 15 56 316 245 2349 256 1688
Fragments arranged by size:
2349 1688 316 270 256 245 56 15
AseI AT'TA_AT
Cuts at: 20 20
Size: 5195
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5195
AvaI C'yCGr_G
Cuts at: 296 701 713 741 778 1091 1700 2215 296
Size: 405 12 28 37 313 609 515 3276
Fragments arranged by size:
3276 609 515 405 313 37 28 12
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2103 2136 2103
Size: 33 5162
BamHI G'GATC_C
Cuts at: 2220 2220
Size: 5195
BanI G'GyrC_C
Cuts at: 1288 2153 2211 2625 2873 2908 1288
Size: 865 58 414 248 35 3575
Fragments arranged by size:
3575 865 414 248 58 35
BanII G_rGCy'C
Cuts at: 436 689 708 742 835 898 964 2209 3239
Size: 253 19 34 93 63 66 1245 1030
Cuts at: 3239 436
Size: 2392
Fragments arranged by size:
2392 1245 1030 253 93 66 63 34
19
BbeI G_GCGC'C
Cuts at: 2629 2877 2629
Size: 248 4947
BbsI GAAGACnn'nnnn_
Cuts at: 634 757 1540 634
Size: 123 783 4289
Fragments arranged by size:
4289 783 123
BbvCI CC'TCA_GC
Cuts at: 974 974
Size: 5195
BciVI GTATCCnnnnn_n'
Cuts at: 3093 3854 5195 3093
Size: 761 1341 3093
Fragments arranged by size:
3093 1341 761
BclI T'GATC_A
Cuts at: 2715 2715
Size: 5195
BglI GCCn_nnn'nGGC
Cuts at: 234 820 234
Size: 586 4609
BglII A'GATC_T
Cuts at: 2710 4747 2710
Size: 2037 3158
BlpI GC'TnA_GC
Cuts at: 2307 2307
Size: 5195
Bme1580I G_kGCm'C
Cuts at: 742 1293 1342 1835 2820 2913 3969 742
Size: 551 49 493 985 93 1056 1968
Fragments arranged by size:
1968 1056 985 551 493 93 49
BmrI ACTGGGnnnn_n'
Cuts at: 149 2407 2488 2823 4967 149
Size: 2258 81 335 2144 377
Fragments arranged by size:
2258 2144 377 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3562 3562
Size: 5195
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 401 587 692 870 899 918 1675 178
Size: 223 186 105 178 29 19 757 3698
Fragments arranged by size:
3698 757 223 186 178 105 29 19
Bpu10I CC'TnA_GC
Cuts at: 683 974 683
Size: 291 4904
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1594 2371 3250 3742 4040 1594
Size: 777 879 492 298 2749
Fragments arranged by size:
2749 879 777 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 487 1483 1588 1779 4899 487
Size: 996 105 191 3120 783
Fragments arranged by size:
3120 996 783 191 105
BsaAI yAC'GTr
Cuts at: 2178 2548 2178
Size: 370 4825
BsaBI GATnn'nnATC
Cuts at: 6 2709 4746 6
Size: 2703 2037 455
Fragments arranged by size:
2703 2037 455
BsaHI Gr'CG_yC
Cuts at: 339 2626 2874 3576 5072 339
Size: 2287 248 702 1496 462
Fragments arranged by size:
2287 1496 702 462 248
BsaWI w'CCGG_w
Cuts at: 1165 1893 2384 2905 3583 3857 4004 4415 1165
Size: 728 491 521 678 274 147 411 1945
Fragments arranged by size:
1945 728 678 521 491 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 374 404 587 617 1312 1342 374
Size: 30 183 30 695 30 4227
Fragments arranged by size:
4227 695 183 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 280 1111 1249 1252 1768 1978 280
Size: 831 138 3 516 210 3497
Fragments arranged by size:
3497 831 516 210 138 3
BseYI C'CCAG_C
Cuts at: 420 429 638 765 857 897 963 1125 1199
Size: 9 209 127 92 40 66 162 74
Cuts at: 1199 1681 2073 2620 3378 3955 4451 420
Size: 482 392 547 758 577 496 1164
Fragments arranged by size:
1164 758 577 547 496 482 392 209
162 127 92 74 66 40 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 459 976 1393 459
Size: 517 417 4261
Fragments arranged by size:
4261 517 417
BsiEI CG_ry'CG
Cuts at: 104 2737 2783 3991 104
Size: 2633 46 1208 1308
Fragments arranged by size:
2633 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 835 1342 1835 2209 2987 3177 3969 835
Size: 507 493 374 778 190 792 2061
Fragments arranged by size:
2061 792 778 507 493 374 190
BsmI GAATG_Cn'
Cuts at: 510 510
Size: 5195
BsmBI CGTCTCn'nnnn_
Cuts at: 903 903
Size: 5195
Bsp1286I G_dGCh'C
Cuts at: 436 689 708 742 835 898 964 1293 1342
Size: 253 19 34 93 63 66 329 49
Cuts at: 1342 1835 2209 2820 2913 2987 3177 3239 3969
Size: 493 374 611 93 74 190 62 730
Cuts at: 3969 436
Size: 1662
Fragments arranged by size:
1662 730 611 493 374 329 253 190
93 93 74 66 63 62 49 34
19
BspEI T'CCGG_A
Cuts at: 1165 4415 1165
Size: 3250 1945
BspHI T'CATG_A
Cuts at: 4371 4371
Size: 5195
BspMI ACCTGCnnnn'nnnn_
Cuts at: 892 948 1719 2245 2459 2761 3142 892
Size: 56 771 526 214 302 381 2945
Fragments arranged by size:
2945 771 526 381 302 214 56
BsrBI CCG'CTC
Cuts at: 50 3487 3541 5025 5190 50
Size: 3437 54 1484 165 55
Fragments arranged by size:
3437 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3107 3107
Size: 5195
BsrFI r'CCGG_y
Cuts at: 161 3193 3374 3583 161
Size: 3032 181 209 1773
Fragments arranged by size:
3032 1773 209 181
BsrGI T'GTAC_A
Cuts at: 1784 1784
Size: 5195
BssSI C'ACGA_G
Cuts at: 1495 3466 3824 1495
Size: 1971 358 2866
Fragments arranged by size:
2866 1971 358
BstAPI GCAn_nnn'nTGC
Cuts at: 482 533 1416 2572 482
Size: 51 883 1156 3105
Fragments arranged by size:
3105 1156 883 51
BstBI TT'CG_AA
Cuts at: 3556 3556
Size: 5195
BstXI CCAn_nnnn'nTGG
Cuts at: 220 220
Size: 5195
BstYI r'GATC_y
Cuts at: 1 1544 1766 2078 2220 2687 2710 3045 3291
Size: 1543 222 312 142 467 23 335 246
Cuts at: 3291 4303 4389 4401 4747 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1543 1012 467 449 346 335 312 246
222 142 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1540 1540
Size: 5195
BtgI C'CryG_G
Cuts at: 1370 1844 1370
Size: 474 4721
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3145 3326 3489 3145
Size: 181 163 4851
Fragments arranged by size:
4851 181 163
BtsI GCAGTG_nn'
Cuts at: 1079 1342 2438 1079
Size: 263 1096 3836
Fragments arranged by size:
3836 1096 263
DraI TTT'AAA
Cuts at: 2194 2194
Size: 5195
DraIII CAC_nnn'GTG
Cuts at: 482 482
Size: 5195
DrdI GACnn_nn'nnGTC
Cuts at: 1692 1873 2020 2901 3759 1692
Size: 181 147 881 858 3128
Fragments arranged by size:
3128 881 858 181 147
EaeI y'GGCC_r
Cuts at: 1572 2780 2954 3345 3372 4717 1572
Size: 1208 174 391 27 1345 2050
Fragments arranged by size:
2050 1345 1208 391 174 27
EagI C'GGCC_G
Cuts at: 2780 2780
Size: 5195
EarI CTCTTCn'nnn_
Cuts at: 245 308 1238 1718 3218 3428 245
Size: 63 930 480 1500 210 2012
Fragments arranged by size:
2012 1500 930 480 210 63
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3403 3713 3859 5005 3403
Size: 310 146 1146 3593
Fragments arranged by size:
3593 1146 310 146
EcoICRI GAG'CTC
Cuts at: 833 2207 833
Size: 1374 3821
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 401 587 692 870 899 918 1384 1639
Size: 223 186 105 178 29 19 466 255
Cuts at: 1639 1675 3020 3452 4199 4467 178
Size: 36 1345 432 747 268 906
Fragments arranged by size:
1345 906 747 466 432 268 255 223
186 178 105 36 29 19
EcoNI CCTnn'n_nnAGG
Cuts at: 944 1717 944
Size: 773 4422
EcoO109I rG'GnC_Cy
Cuts at: 600 738 757 812 992 1015 1359 2334 600
Size: 138 19 55 180 23 344 975 3461
Fragments arranged by size:
3461 975 344 180 138 55 23 19
EcoRI G'AATT_C
Cuts at: 1297 1954 2199 1297
Size: 657 245 4293
Fragments arranged by size:
4293 657 245
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1959 3214 1959
Size: 1255 3940
FspI TGC'GCA
Cuts at: 808 2976 808
Size: 2168 3027
HaeII r_GCGC'y
Cuts at: 970 2100 2151 2629 2877 3899 4878 970
Size: 1130 51 478 248 1022 979 1287
Fragments arranged by size:
1287 1130 1022 979 478 248 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 754 786 1312 1344 1592 1624 3037 3067 3069
Size: 32 526 32 248 32 1413 30 2
Cuts at: 3069 3099 754
Size: 30 2850
Fragments arranged by size:
2850 1413 526 248 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2234 2234
Size: 5195
HindIII A'AGCT_T
Cuts at: 1441 1441
Size: 5195
KasI G'GCGC_C
Cuts at: 2625 2873 2625
Size: 248 4947
KpnI G_GTAC'C
Cuts at: 2215 2215
Size: 5195
MluI A'CGCG_T
Cuts at: 4628 4628
Size: 5195
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 124 1113 1460 3866 4050 124
Size: 989 347 2406 184 1269
Fragments arranged by size:
2406 1269 989 347 184
MscI TGG'CCA
Cuts at: 1574 2956 1574
Size: 1382 3813
MslI CAynn'nnrTG
Cuts at: 218 3184 3311 218
Size: 2966 127 2102
Fragments arranged by size:
2966 2102 127
MspA1I CmG'CkG
Cuts at: 398 420 1192 2073 2306 2620 2980 3993 4238
Size: 22 772 881 233 314 360 1013 245
Cuts at: 4238 398
Size: 1355
Fragments arranged by size:
1355 1013 881 772 360 314 245 233
22
NaeI GCC'GGC
Cuts at: 3376 3376
Size: 5195
NarI GG'CG_CC
Cuts at: 2626 2874 2626
Size: 248 4947
NcoI C'CATG_G
Cuts at: 1370 1844 1370
Size: 474 4721
NgoMIV G'CCGG_C
Cuts at: 3374 3374
Size: 5195
NspI r_CATG'y
Cuts at: 1527 2248 2748 3655 1527
Size: 721 500 907 3067
Fragments arranged by size:
3067 907 721 500
PasI CC'CwG_GG
Cuts at: 1072 1284 1072
Size: 212 4983
PciI A'CATG_T
Cuts at: 3651 3651
Size: 5195
PflMI CCAn_nnn'nTGG
Cuts at: 1132 1132
Size: 5195
PmeI GTTT'AAAC
Cuts at: 2194 2194
Size: 5195
PmlI CAC'GTG
Cuts at: 2178 2178
Size: 5195
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4334 4366 4334
Size: 32 5163
PpuMI rG'GwC_Cy
Cuts at: 600 992 1015 600
Size: 392 23 4780
Fragments arranged by size:
4780 392 23
PspOMI G'GGCC_C
Cuts at: 738 738
Size: 5195
PspXI vC'TCGA_Gb
Cuts at: 296 713 296
Size: 417 4778
PstI C_TGCA'G
Cuts at: 1191 1212 1337 2242 2927 1191
Size: 21 125 905 685 3459
Fragments arranged by size:
3459 905 685 125 21
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5195
PvuII CAG'CTG
Cuts at: 420 1192 2073 2620 2980 420
Size: 772 881 547 360 2635
Fragments arranged by size:
2635 881 772 547 360
RsrII CG'GwC_CG
Cuts at: 3390 3390
Size: 5195
SacI G_AGCT'C
Cuts at: 835 2209 835
Size: 1374 3821
SalI G'TCGA_C
Cuts at: 2232 2232
Size: 5195
SanDI GG'GwC_CC
Cuts at: 992 992
Size: 5195
SapI GCTCTTCn'nnn_
Cuts at: 245 1718 3218 3428 245
Size: 1473 1500 210 2012
Fragments arranged by size:
2012 1500 1473 210
SbfI CC_TGCA'GG
Cuts at: 2242 2242
Size: 5195
ScaI AGT'ACT
Cuts at: 4712 4712
Size: 5195
SfcI C'TryA_G
Cuts at: 33 1187 1208 1333 2161 2238 2923 3916 4107
Size: 1154 21 125 828 77 685 993 191
Cuts at: 4107 33
Size: 1121
Fragments arranged by size:
1154 1121 993 828 685 191 125 77
21
SfoI GGC'GCC
Cuts at: 2627 2875 2627
Size: 248 4947
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5195
SmaI CCC'GGG
Cuts at: 703 743 780 2217 703
Size: 40 37 1437 3681
Fragments arranged by size:
3681 1437 40 37
SmlI C'TyrA_G
Cuts at: 296 713 1609 2350 3265 3757 4019 296
Size: 417 896 741 915 492 262 1472
Fragments arranged by size:
1472 915 896 741 492 417 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5195
SphI G_CATG'C
Cuts at: 2248 2748 2248
Size: 500 4695
SrfI GCCC'GGGC
Cuts at: 703 703
Size: 5195
StuI AGG'CCT
Cuts at: 931 1805 931
Size: 874 4321
StyI C'CwwG_G
Cuts at: 1355 1370 1844 2087 2329 2683 1355
Size: 15 474 243 242 354 3867
Fragments arranged by size:
3867 474 354 243 242 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1163 1276 2778 2911 3370 3579 1163
Size: 113 1502 133 459 209 2779
Fragments arranged by size:
2779 1502 459 209 133 113
TatI w'GTAC_w
Cuts at: 1784 4710 1784
Size: 2926 2269
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 329 361 374 406 4712 4744 329
Size: 32 13 32 4306 32 780
Fragments arranged by size:
4306 780 32 32 32 13
Tth111I GACn'n_nGTC
Cuts at: 1013 2992 1013
Size: 1979 3216
XbaI T'CTAG_A
Cuts at: 2226 2226
Size: 5195
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 486 2690 486
Size: 2204 2991
XhoI C'TCGA_G
Cuts at: 296 713 296
Size: 417 4778
XmaI C'CCGG_G
Cuts at: 701 741 778 2215 701
Size: 40 37 1437 3681
Fragments arranged by size:
3681 1437 40 37
XmnI GAAnn'nnTTC
Cuts at: 1639 1639
Size: 5195
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SrfI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AloI AscI AvrII BcgI BfrBI
BmgBI BmtI BsiWI BssHII BstEII BstZ17I ClaI CspCI
EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PfoI PshAI PsiI PsrI SacII
SexAI SfiI SgrAI SnaBI SspI SwaI ZraI