Restriction map for
pF1KA0994
(Circular) (Six-base) MAPSORT of: pF1KA0994.seq Check: 5298 from: 1 to: 5108
pF1KA0994 5108 bp
With 182 enzymes: SgfI *
March 2, 2010 14:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1461 1461
Size: 5108
AccI GT'mk_AC
Cuts at: 194 623 2146 194
Size: 429 1523 3156
Fragments arranged by size:
3156 1523 429
Acc65I G'GTAC_C
Cuts at: 2124 2124
Size: 5108
AclI AA'CG_TT
Cuts at: 4948 4948
Size: 5108
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 210 253 1132 1480 2933 3365 4112 4380 210
Size: 43 879 348 1453 432 747 268 938
Fragments arranged by size:
1453 938 879 747 432 348 268 43
AfeI AGC'GCT
Cuts at: 310 1744 310
Size: 1434 3674
AflIII A'CryG_T
Cuts at: 246 248 647 2460 3564 4541 246
Size: 2 399 1813 1104 977 813
Fragments arranged by size:
1813 1104 977 813 399 2
AgeI A'CCGG_T
Cuts at: 3496 3496
Size: 5108
AleI CACnn'nnGTG
Cuts at: 1961 1961
Size: 5108
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 352 352
Size: 5108
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 751 783 838 870 751
Size: 32 55 32 4989
Fragments arranged by size:
4989 55 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2413 3980 2413
Size: 1567 3541
ApaI G_GGCC'C
Cuts at: 219 538 1270 219
Size: 319 732 4057
Fragments arranged by size:
4057 732 319
ApaLI G'TGCA_C
Cuts at: 3878 3878
Size: 5108
ApoI r'AATT_y
Cuts at: 1564 2026 2112 4461 4717 1564
Size: 462 86 2349 256 1955
Fragments arranged by size:
2349 1955 462 256 86
AseI AT'TA_AT
Cuts at: 20 20
Size: 5108
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5108
AvaI C'yCGr_G
Cuts at: 538 1223 1289 2128 538
Size: 685 66 839 3518
Fragments arranged by size:
3518 839 685 66
BamHI G'GATC_C
Cuts at: 1151 2133 1151
Size: 982 4126
BanI G'GyrC_C
Cuts at: 172 1479 2050 2097 2124 2538 2786 2821 172
Size: 1307 571 47 27 414 248 35 2459
Fragments arranged by size:
2459 1307 571 414 248 47 35 27
BanII G_rGCy'C
Cuts at: 219 440 538 1262 1270 1298 1493 1979 2122
Size: 221 98 724 8 28 195 486 143
Cuts at: 2122 3152 219
Size: 1030 2175
Fragments arranged by size:
2175 1030 724 486 221 195 143 98
28 8
BbeI G_GCGC'C
Cuts at: 2542 2790 2542
Size: 248 4860
BbvCI CC'TCA_GC
Cuts at: 281 281
Size: 5108
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 405 439 1380 1414 1983 1996 2017
Size: 34 281 34 941 34 569 13 21
Cuts at: 2017 2030 90
Size: 13 3168
Fragments arranged by size:
3168 941 569 281 34 34 34 21
13 13
BciVI GTATCCnnnnn_n'
Cuts at: 691 3006 3767 5108 691
Size: 2315 761 1341 691
Fragments arranged by size:
2315 1341 761 691
BclI T'GATC_A
Cuts at: 2628 2628
Size: 5108
BglI GCCn_nnn'nGGC
Cuts at: 542 1920 542
Size: 1378 3730
BglII A'GATC_T
Cuts at: 458 636 2623 4660 458
Size: 178 1987 2037 906
Fragments arranged by size:
2037 1987 906 178
BlpI GC'TnA_GC
Cuts at: 2220 2220
Size: 5108
Bme1580I G_kGCm'C
Cuts at: 175 219 538 1270 2102 2733 2826 3882 175
Size: 44 319 732 832 631 93 1056 1401
Fragments arranged by size:
1401 1056 832 732 631 319 93 44
BmrI ACTGGGnnnn_n'
Cuts at: 1348 2320 2401 2736 4880 1348
Size: 972 81 335 2144 1576
Fragments arranged by size:
2144 1576 972 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 909 1512 3475 909
Size: 603 1963 2542
Fragments arranged by size:
2542 1963 603
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 946 1360 1969 1993 946
Size: 414 609 24 4061
Fragments arranged by size:
4061 609 414 24
Bpu10I CC'TnA_GC
Cuts at: 281 281
Size: 5108
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 625 1756 2284 3163 3655 3953 625
Size: 1131 528 879 492 298 1780
Fragments arranged by size:
1780 1131 879 528 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 909 4812 909
Size: 3903 1205
BsaAI yAC'GTr
Cuts at: 165 249 756 1284 1521 1959 2461 165
Size: 84 507 528 237 438 502 2812
Fragments arranged by size:
2812 528 507 502 438 237 84
BsaBI GATnn'nnATC
Cuts at: 6 2622 4659 6
Size: 2616 2037 455
Fragments arranged by size:
2616 2037 455
BsaHI Gr'CG_yC
Cuts at: 2539 2787 3489 4985 2539
Size: 248 702 1496 2662
Fragments arranged by size:
2662 1496 702 248
BsaWI w'CCGG_w
Cuts at: 983 2297 2818 3496 3770 3917 4328 983
Size: 1314 521 678 274 147 411 1763
Fragments arranged by size:
1763 1314 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 145 175 751 781 838 868 1717 1747 145
Size: 30 576 30 57 30 849 30 3506
Fragments arranged by size:
3506 849 576 57 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 298 1948 298
Size: 1650 3458
BseYI C'CCAG_C
Cuts at: 168 207 325 1466 1782 2533 3291 3868 4364
Size: 39 118 1141 316 751 758 577 496
Cuts at: 4364 168
Size: 912
Fragments arranged by size:
1141 912 758 751 577 496 316 118
39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1495 1741 1495
Size: 246 4862
BsiEI CG_ry'CG
Cuts at: 104 684 1146 2650 2696 3904 104
Size: 580 462 1504 46 1208 1308
Fragments arranged by size:
1504 1308 1208 580 462 46
BsiHKAI G_wGCw'C
Cuts at: 364 1262 1493 2122 2900 3090 3882 364
Size: 898 231 629 778 190 792 1590
Fragments arranged by size:
1590 898 792 778 629 231 190
BsmI GAATG_Cn'
Cuts at: 416 1945 1996 416
Size: 1529 51 3528
Fragments arranged by size:
3528 1529 51
Bsp1286I G_dGCh'C
Cuts at: 175 219 364 440 538 1262 1270 1298 1493
Size: 44 145 76 98 724 8 28 195
Cuts at: 1493 1979 2102 2122 2733 2826 2900 3090 3152
Size: 486 123 20 611 93 74 190 62
Cuts at: 3152 3882 175
Size: 730 1401
Fragments arranged by size:
1401 730 724 611 486 195 190 145
123 98 93 76 74 62 44 28
20 8
BspEI T'CCGG_A
Cuts at: 983 4328 983
Size: 3345 1763
BspHI T'CATG_A
Cuts at: 1001 4284 1001
Size: 3283 1825
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1461 1820 2158 2372 2674 3055 1461
Size: 359 338 214 302 381 3514
Fragments arranged by size:
3514 381 359 338 302 214
BsrBI CCG'CTC
Cuts at: 50 120 1819 3400 3454 4938 5103 50
Size: 70 1699 1581 54 1484 165 55
Fragments arranged by size:
1699 1581 1484 165 70 55 54
BsrDI GCAATG_nn'
Cuts at: 3020 3020
Size: 5108
BsrFI r'CCGG_y
Cuts at: 800 3106 3287 3496 800
Size: 2306 181 209 2412
Fragments arranged by size:
2412 2306 209 181
BsrGI T'GTAC_A
Cuts at: 713 713
Size: 5108
BssSI C'ACGA_G
Cuts at: 3379 3737 3379
Size: 358 4750
BstAPI GCAn_nnn'nTGC
Cuts at: 2097 2485 2097
Size: 388 4720
BstBI TT'CG_AA
Cuts at: 3469 3469
Size: 5108
BstEII G'GTnAC_C
Cuts at: 342 384 342
Size: 42 5066
BstXI CCAn_nnnn'nTGG
Cuts at: 561 561
Size: 5108
BstYI r'GATC_y
Cuts at: 1 458 636 1151 2133 2600 2623 2958 3204
Size: 457 178 515 982 467 23 335 246
Cuts at: 3204 4216 4302 4314 4660 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 982 515 467 457 449 346 335
246 178 86 23 12
BtgI C'CryG_G
Cuts at: 842 994 1205 1484 1632 842
Size: 152 211 279 148 4318
Fragments arranged by size:
4318 279 211 152 148
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3058 3239 3402 3058
Size: 181 163 4764
Fragments arranged by size:
4764 181 163
BtsI GCAGTG_nn'
Cuts at: 381 2351 381
Size: 1970 3138
DraI TTT'AAA
Cuts at: 2107 2107
Size: 5108
DraIII CAC_nnn'GTG
Cuts at: 2097 2097
Size: 5108
DrdI GACnn_nn'nnGTC
Cuts at: 2814 3672 2814
Size: 858 4250
EaeI y'GGCC_r
Cuts at: 290 774 1386 1580 1629 2693 2867 3258 3285
Size: 484 612 194 49 1064 174 391 27
Cuts at: 3285 4630 290
Size: 1345 768
Fragments arranged by size:
1345 1064 768 612 484 391 194 174
49 27
EagI C'GGCC_G
Cuts at: 2693 2693
Size: 5108
EarI CTCTTCn'nnn_
Cuts at: 445 1612 3131 3341 445
Size: 1167 1519 210 2212
Fragments arranged by size:
2212 1519 1167 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 819 3316 3626 3772 4918 819
Size: 2497 310 146 1146 1009
Fragments arranged by size:
2497 1146 1009 310 146
EcoICRI GAG'CTC
Cuts at: 1260 1491 2120 1260
Size: 231 629 4248
Fragments arranged by size:
4248 629 231
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 210 253 946 1132 1360 1480 1969 1993 2933
Size: 43 693 186 228 120 489 24 940
Cuts at: 2933 3365 4112 4380 210
Size: 432 747 268 938
Fragments arranged by size:
940 938 747 693 489 432 268 228
186 120 43 24
EcoNI CCTnn'n_nnAGG
Cuts at: 1072 1720 1774 1072
Size: 648 54 4406
Fragments arranged by size:
4406 648 54
EcoO109I rG'GnC_Cy
Cuts at: 215 534 535 676 1185 1896 2247 215
Size: 319 1 141 509 711 351 3076
Fragments arranged by size:
3076 711 509 351 319 141 1
EcoRI G'AATT_C
Cuts at: 2112 2112
Size: 5108
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1137 3127 1137
Size: 1990 3118
FspI TGC'GCA
Cuts at: 2889 2889
Size: 5108
HaeII r_GCGC'y
Cuts at: 312 1746 2542 2790 3812 4791 312
Size: 1434 796 248 1022 979 629
Fragments arranged by size:
1434 1022 979 796 629 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1519 1551 1628 1660 2950 2980 2982 3012 1519
Size: 32 77 32 1290 30 2 30 3615
Fragments arranged by size:
3615 1290 77 32 32 30 30 2
HincII GTy'rAC
Cuts at: 393 2147 393
Size: 1754 3354
KasI G'GCGC_C
Cuts at: 2538 2786 2538
Size: 248 4860
KpnI G_GTAC'C
Cuts at: 2128 2128
Size: 5108
MluI A'CGCG_T
Cuts at: 4541 4541
Size: 5108
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1129 1408 1730 3779 3963 1129
Size: 279 322 2049 184 2274
Fragments arranged by size:
2274 2049 322 279 184
MscI TGG'CCA
Cuts at: 292 1582 2869 292
Size: 1290 1287 2531
Fragments arranged by size:
2531 1290 1287
MslI CAynn'nnrTG
Cuts at: 721 1523 1882 1961 3097 3224 721
Size: 802 359 79 1136 127 2605
Fragments arranged by size:
2605 1136 802 359 127 79
MspA1I CmG'CkG
Cuts at: 844 2219 2533 2893 3906 4151 844
Size: 1375 314 360 1013 245 1801
Fragments arranged by size:
1801 1375 1013 360 314 245
NaeI GCC'GGC
Cuts at: 802 3289 802
Size: 2487 2621
NarI GG'CG_CC
Cuts at: 2539 2787 2539
Size: 248 4860
NcoI C'CATG_G
Cuts at: 1632 1632
Size: 5108
NgoMIV G'CCGG_C
Cuts at: 800 3287 800
Size: 2487 2621
NspI r_CATG'y
Cuts at: 651 2161 2661 3568 651
Size: 1510 500 907 2191
Fragments arranged by size:
2191 1510 907 500
PasI CC'CwG_GG
Cuts at: 1980 1980
Size: 5108
PciI A'CATG_T
Cuts at: 647 3564 647
Size: 2917 2191
PflMI CCAn_nnn'nTGG
Cuts at: 795 1276 795
Size: 481 4627
PfoI T'CCnGG_A
Cuts at: 523 1154 1970 523
Size: 631 816 3661
Fragments arranged by size:
3661 816 631
PmeI GTTT'AAAC
Cuts at: 2107 2107
Size: 5108
PmlI CAC'GTG
Cuts at: 249 1521 1959 249
Size: 1272 438 3398
Fragments arranged by size:
3398 1272 438
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 751 783 838 870 4247 4279 751
Size: 32 55 32 3377 32 1580
Fragments arranged by size:
3377 1580 55 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 1896 1896
Size: 5108
PshAI GACnn'nnGTC
Cuts at: 190 190
Size: 5108
PspOMI G'GGCC_C
Cuts at: 215 534 1266 215
Size: 319 732 4057
Fragments arranged by size:
4057 732 319
PstI C_TGCA'G
Cuts at: 137 2155 2840 137
Size: 2018 685 2405
Fragments arranged by size:
2405 2018 685
PvuI CG_AT'CG
Cuts at: 104 1146 104
Size: 1042 4066
PvuII CAG'CTG
Cuts at: 2533 2893 2533
Size: 360 4748
RsrII CG'GwC_CG
Cuts at: 122 3303 122
Size: 3181 1927
SacI G_AGCT'C
Cuts at: 1262 1493 2122 1262
Size: 231 629 4248
Fragments arranged by size:
4248 629 231
SacII CC_GC'GG
Cuts at: 845 845
Size: 5108
SalI G'TCGA_C
Cuts at: 2145 2145
Size: 5108
SapI GCTCTTCn'nnn_
Cuts at: 445 1612 3131 3341 445
Size: 1167 1519 210 2212
Fragments arranged by size:
2212 1519 1167 210
SbfI CC_TGCA'GG
Cuts at: 2155 2155
Size: 5108
ScaI AGT'ACT
Cuts at: 4625 4625
Size: 5108
SfcI C'TryA_G
Cuts at: 33 133 195 624 2151 2836 3829 4020 33
Size: 100 62 429 1527 685 993 191 1121
Fragments arranged by size:
1527 1121 993 685 429 191 100 62
SfiI GGCCn_nnn'nGGCC
Cuts at: 542 542
Size: 5108
SfoI GGC'GCC
Cuts at: 2540 2788 2540
Size: 248 4860
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5108
SmaI CCC'GGG
Cuts at: 1291 2130 1291
Size: 839 4269
SmlI C'TyrA_G
Cuts at: 640 1771 2263 3178 3670 3932 640
Size: 1131 492 915 492 262 1816
Fragments arranged by size:
1816 1131 915 492 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5108
SphI G_CATG'C
Cuts at: 2161 2661 2161
Size: 500 4608
StuI AGG'CCT
Cuts at: 869 899 869
Size: 30 5078
StyI C'CwwG_G
Cuts at: 596 1439 1632 2242 2596 596
Size: 843 193 610 354 3108
Fragments arranged by size:
3108 843 610 354 193
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 202 243 590 1651 2691 2824 3283 3492 202
Size: 41 347 1061 1040 133 459 209 1818
Fragments arranged by size:
1818 1061 1040 459 347 209 133 41
TatI w'GTAC_w
Cuts at: 713 827 929 1733 4623 713
Size: 114 102 804 2890 1198
Fragments arranged by size:
2890 1198 804 114 102
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 235 267 4625 4657 235
Size: 32 4358 32 686
Fragments arranged by size:
4358 686 32 32
Tth111I GACn'n_nGTC
Cuts at: 382 2905 382
Size: 2523 2585
XbaI T'CTAG_A
Cuts at: 2139 2139
Size: 5108
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2603 2603
Size: 5108
XmaI C'CCGG_G
Cuts at: 1289 2128 1289
Size: 839 4269
XmnI GAAnn'nnTTC
Cuts at: 766 2071 766
Size: 1305 3803
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AscI AvrII BaeI BbsI BfrBI
BmgBI BmtI BsiWI BsmBI BssHII BstZ17I Bsu36I ClaI
CspCI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PsiI PspXI PsrI
SanDI SexAI SgrAI SnaBI SrfI SspI SwaI XhoI
ZraI