Restriction map for
pF1KA0996
(Circular) (Six-base) MAPSORT of: pF1KA0996.seq Check: 2726 from: 1 to: 5657
pF1KA0996 5657 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2505 2505
Size: 5657
AatII G_ACGT'C
Cuts at: 200 200
Size: 5657
AccI GT'mk_AC
Cuts at: 161 2695 161
Size: 2534 3123
Acc65I G'GTAC_C
Cuts at: 2673 2673
Size: 5657
AclI AA'CG_TT
Cuts at: 5497 5497
Size: 5657
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 481 794 1874 2627 3482 3914 4661 4929 481
Size: 313 1080 753 855 432 747 268 1209
Fragments arranged by size:
1209 1080 855 753 747 432 313 268
AflIII A'CryG_T
Cuts at: 3009 4113 5090 3009
Size: 1104 977 3576
Fragments arranged by size:
3576 1104 977
AgeI A'CCGG_T
Cuts at: 4045 4045
Size: 5657
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 607 607
Size: 5657
AlwNI CAG_nnn'CTG
Cuts at: 672 2962 4529 672
Size: 2290 1567 1800
Fragments arranged by size:
2290 1800 1567
ApaI G_GGCC'C
Cuts at: 194 269 194
Size: 75 5582
ApaLI G'TGCA_C
Cuts at: 4427 4427
Size: 5657
ApoI r'AATT_y
Cuts at: 780 1039 1062 1829 1837 2661 5010 5266 780
Size: 259 23 767 8 824 2349 256 1171
Fragments arranged by size:
2349 1171 824 767 259 256 23 8
AseI AT'TA_AT
Cuts at: 20 1724 20
Size: 1704 3953
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5657
AvaI C'yCGr_G
Cuts at: 1235 2677 1235
Size: 1442 4215
BamHI G'GATC_C
Cuts at: 2682 2682
Size: 5657
BanI G'GyrC_C
Cuts at: 2673 3087 3335 3370 2673
Size: 414 248 35 4960
Fragments arranged by size:
4960 414 248 35
BanII G_rGCy'C
Cuts at: 194 269 2671 3701 194
Size: 75 2402 1030 2150
Fragments arranged by size:
2402 2150 1030 75
BbeI G_GCGC'C
Cuts at: 3091 3339 3091
Size: 248 5409
BbsI GAAGACnn'nnnn_
Cuts at: 419 953 1166 2057 2426 419
Size: 534 213 891 369 3650
Fragments arranged by size:
3650 891 534 369 213
BbvCI CC'TCA_GC
Cuts at: 116 116
Size: 5657
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1523 1557 90
Size: 34 1399 34 4190
Fragments arranged by size:
4190 1399 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1911 3555 4316 5657 1911
Size: 1644 761 1341 1911
Fragments arranged by size:
1911 1644 1341 761
BclI T'GATC_A
Cuts at: 1752 3177 1752
Size: 1425 4232
BfrBI ATG'CAT
Cuts at: 1626 1626
Size: 5657
BglI GCCn_nnn'nGGC
Cuts at: 235 235
Size: 5657
BglII A'GATC_T
Cuts at: 3172 5209 3172
Size: 2037 3620
BlpI GC'TnA_GC
Cuts at: 322 2769 322
Size: 2447 3210
Bme1580I G_kGCm'C
Cuts at: 194 269 427 3282 3375 4431 194
Size: 75 158 2855 93 1056 1420
Fragments arranged by size:
2855 1420 1056 158 93 75
BmgBI CAC'GTC
Cuts at: 336 363 336
Size: 27 5630
BmrI ACTGGGnnnn_n'
Cuts at: 2433 2869 2950 3285 5429 2433
Size: 436 81 335 2144 2661
Fragments arranged by size:
2661 2144 436 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4024 4024
Size: 5657
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 319 562 1366 1952 319
Size: 243 804 586 4024
Fragments arranged by size:
4024 804 586 243
Bpu10I CC'TnA_GC
Cuts at: 116 559 116
Size: 443 5214
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 610 2833 3712 4204 4502 610
Size: 2223 879 492 298 1765
Fragments arranged by size:
2223 1765 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5361 5361
Size: 5657
BsaAI yAC'GTr
Cuts at: 1738 3010 1738
Size: 1272 4385
BsaBI GATnn'nnATC
Cuts at: 6 2368 3171 5208 6
Size: 2362 803 2037 455
Fragments arranged by size:
2362 2037 803 455
BsaHI Gr'CG_yC
Cuts at: 197 3088 3336 4038 5534 197
Size: 2891 248 702 1496 320
Fragments arranged by size:
2891 1496 702 320 248
BsaWI w'CCGG_w
Cuts at: 824 2846 3367 4045 4319 4466 4877 824
Size: 2022 521 678 274 147 411 1604
Fragments arranged by size:
2022 1604 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2067 2097 2261 2291 2067
Size: 30 164 30 5433
Fragments arranged by size:
5433 164 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 559 1644 1885 2113 2116 559
Size: 1085 241 228 3 4100
Fragments arranged by size:
4100 1085 241 228 3
BseYI C'CCAG_C
Cuts at: 250 665 1479 3082 3840 4417 4913 250
Size: 415 814 1603 758 577 496 994
Fragments arranged by size:
1603 994 814 758 577 496 415
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 487 517 869 920 1471 1920 487
Size: 30 352 51 551 449 4224
Fragments arranged by size:
4224 551 449 352 51 30
BsiEI CG_ry'CG
Cuts at: 104 3199 3245 4453 104
Size: 3095 46 1208 1308
Fragments arranged by size:
3095 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2671 3449 3639 4431 2671
Size: 778 190 792 3897
Fragments arranged by size:
3897 792 778 190
BsmI GAATG_Cn'
Cuts at: 1479 1534 1479
Size: 55 5602
Bsp1286I G_dGCh'C
Cuts at: 194 269 427 2671 3282 3375 3449 3639 3701
Size: 75 158 2244 611 93 74 190 62
Cuts at: 3701 4431 194
Size: 730 1420
Fragments arranged by size:
2244 1420 730 611 190 158 93 75
74 62
BspEI T'CCGG_A
Cuts at: 824 4877 824
Size: 4053 1604
BspHI T'CATG_A
Cuts at: 386 4833 386
Size: 4447 1210
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2505 2707 2921 3223 3604 2505
Size: 202 214 302 381 4558
Fragments arranged by size:
4558 381 302 214 202
BsrBI CCG'CTC
Cuts at: 50 171 549 3949 4003 5487 5652 50
Size: 121 378 3400 54 1484 165 55
Fragments arranged by size:
3400 1484 378 165 121 55 54
BsrDI GCAATG_nn'
Cuts at: 1344 3569 1344
Size: 2225 3432
BsrFI r'CCGG_y
Cuts at: 3655 3836 4045 3655
Size: 181 209 5267
Fragments arranged by size:
5267 209 181
BssSI C'ACGA_G
Cuts at: 1147 3928 4286 1147
Size: 2781 358 2518
Fragments arranged by size:
2781 2518 358
BstAPI GCAn_nnn'nTGC
Cuts at: 892 3034 892
Size: 2142 3515
BstBI TT'CG_AA
Cuts at: 1057 1305 4018 1057
Size: 248 2713 2696
Fragments arranged by size:
2713 2696 248
BstXI CCAn_nnnn'nTGG
Cuts at: 336 336
Size: 5657
BstYI r'GATC_y
Cuts at: 1 2046 2682 3149 3172 3507 3753 4765 4851
Size: 2045 636 467 23 335 246 1012 86
Cuts at: 4851 4863 5209 1
Size: 12 346 449
Fragments arranged by size:
2045 1012 636 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2555 2555
Size: 5657
BtgI C'CryG_G
Cuts at: 218 233 292 218
Size: 15 59 5583
Fragments arranged by size:
5583 59 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2302 3607 3788 3951 2302
Size: 1305 181 163 4008
Fragments arranged by size:
4008 1305 181 163
BtsI GCAGTG_nn'
Cuts at: 429 564 1908 2900 429
Size: 135 1344 992 3186
Fragments arranged by size:
3186 1344 992 135
DraI TTT'AAA
Cuts at: 1044 1155 2656 1044
Size: 111 1501 4045
Fragments arranged by size:
4045 1501 111
DrdI GACnn_nn'nnGTC
Cuts at: 3363 4221 3363
Size: 858 4799
EaeI y'GGCC_r
Cuts at: 206 3242 3416 3807 3834 5179 206
Size: 3036 174 391 27 1345 684
Fragments arranged by size:
3036 1345 684 391 174 27
EagI C'GGCC_G
Cuts at: 3242 3242
Size: 5657
EarI CTCTTCn'nnn_
Cuts at: 626 845 1358 1639 1893 2441 3680 3890 626
Size: 219 513 281 254 548 1239 210 2393
Fragments arranged by size:
2393 1239 548 513 281 254 219 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3865 4175 4321 5467 3865
Size: 310 146 1146 4055
Fragments arranged by size:
4055 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2669 2669
Size: 5657
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 319 481 562 794 1366 1874 1952 2627 3482
Size: 162 81 232 572 508 78 675 855
Cuts at: 3482 3914 4661 4929 319
Size: 432 747 268 1047
Fragments arranged by size:
1047 855 747 675 572 508 432 268
232 162 81 78
EcoNI CCTnn'n_nnAGG
Cuts at: 1663 1663
Size: 5657
EcoO109I rG'GnC_Cy
Cuts at: 190 265 266 2796 190
Size: 75 1 2530 3051
Fragments arranged by size:
3051 2530 75 1
EcoRI G'AATT_C
Cuts at: 1062 1837 2661 1062
Size: 775 824 4058
Fragments arranged by size:
4058 824 775
EcoRV GAT'ATC
Cuts at: 2453 2453
Size: 5657
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1655 2199 3676 1655
Size: 544 1477 3636
Fragments arranged by size:
3636 1477 544
FspI TGC'GCA
Cuts at: 3438 3438
Size: 5657
HaeII r_GCGC'y
Cuts at: 329 3091 3339 4361 5340 329
Size: 2762 248 1022 979 646
Fragments arranged by size:
2762 1022 979 646 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1308 1340 1392 1424 1996 2028 2419 2451 3499
Size: 32 52 32 572 32 391 32 1048
Cuts at: 3499 3529 3531 3561 1308
Size: 30 2 30 3404
Fragments arranged by size:
3404 1048 572 391 52 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2696 2696
Size: 5657
HindIII A'AGCT_T
Cuts at: 737 1637 737
Size: 900 4757
KasI G'GCGC_C
Cuts at: 3087 3335 3087
Size: 248 5409
KpnI G_GTAC'C
Cuts at: 2677 2677
Size: 5657
MluI A'CGCG_T
Cuts at: 5090 5090
Size: 5657
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1145 4328 4512 1145
Size: 3183 184 2290
Fragments arranged by size:
3183 2290 184
MscI TGG'CCA
Cuts at: 3418 3418
Size: 5657
MslI CAynn'nnrTG
Cuts at: 334 713 896 3646 3773 334
Size: 379 183 2750 127 2218
Fragments arranged by size:
2750 2218 379 183 127
MspA1I CmG'CkG
Cuts at: 124 179 220 294 531 672 1859 2768 3082
Size: 55 41 74 237 141 1187 909 314
Cuts at: 3082 3442 4455 4700 124
Size: 360 1013 245 1081
Fragments arranged by size:
1187 1081 1013 909 360 314 245 237
141 74 55 41
NaeI GCC'GGC
Cuts at: 3838 3838
Size: 5657
NarI GG'CG_CC
Cuts at: 3088 3336 3088
Size: 248 5409
NcoI C'CATG_G
Cuts at: 233 233
Size: 5657
NgoMIV G'CCGG_C
Cuts at: 3836 3836
Size: 5657
NsiI A_TGCA'T
Cuts at: 1628 1628
Size: 5657
NspI r_CATG'y
Cuts at: 144 160 2710 3210 4117 144
Size: 16 2550 500 907 1684
Fragments arranged by size:
2550 1684 907 500 16
PciI A'CATG_T
Cuts at: 4113 4113
Size: 5657
PflMI CCAn_nnn'nTGG
Cuts at: 2344 2344
Size: 5657
PfoI T'CCnGG_A
Cuts at: 1382 1382
Size: 5657
PmeI GTTT'AAAC
Cuts at: 2656 2656
Size: 5657
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4796 4828 4796
Size: 32 5625
PsiI TTA'TAA
Cuts at: 1405 1405
Size: 5657
PspOMI G'GGCC_C
Cuts at: 190 265 190
Size: 75 5582
PspXI vC'TCGA_Gb
Cuts at: 1235 1235
Size: 5657
PstI C_TGCA'G
Cuts at: 377 860 2704 3389 377
Size: 483 1844 685 2645
Fragments arranged by size:
2645 1844 685 483
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5657
PvuII CAG'CTG
Cuts at: 531 672 1859 3082 3442 531
Size: 141 1187 1223 360 2746
Fragments arranged by size:
2746 1223 1187 360 141
RsrII CG'GwC_CG
Cuts at: 3852 3852
Size: 5657
SacI G_AGCT'C
Cuts at: 2671 2671
Size: 5657
SacII CC_GC'GG
Cuts at: 221 295 221
Size: 74 5583
SalI G'TCGA_C
Cuts at: 2694 2694
Size: 5657
SapI GCTCTTCn'nnn_
Cuts at: 845 1358 3680 3890 845
Size: 513 2322 210 2612
Fragments arranged by size:
2612 2322 513 210
SbfI CC_TGCA'GG
Cuts at: 2704 2704
Size: 5657
ScaI AGT'ACT
Cuts at: 496 1769 5174 496
Size: 1273 3405 979
Fragments arranged by size:
3405 1273 979
SexAI A'CCwGG_T
Cuts at: 865 865
Size: 5657
SfcI C'TryA_G
Cuts at: 33 373 856 2700 3385 4378 4569 33
Size: 340 483 1844 685 993 191 1121
Fragments arranged by size:
1844 1121 993 685 483 340 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 235 235
Size: 5657
SfoI GGC'GCC
Cuts at: 3089 3337 3089
Size: 248 5409
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5657
SmaI CCC'GGG
Cuts at: 2679 2679
Size: 5657
SmlI C'TyrA_G
Cuts at: 625 1235 2812 3727 4219 4481 625
Size: 610 1577 915 492 262 1801
Fragments arranged by size:
1801 1577 915 610 492 262
SpeI A'CTAG_T
Cuts at: 67 996 67
Size: 929 4728
SphI G_CATG'C
Cuts at: 144 2710 3210 144
Size: 2566 500 2591
Fragments arranged by size:
2591 2566 500
StuI AGG'CCT
Cuts at: 724 724
Size: 5657
StyI C'CwwG_G
Cuts at: 233 914 1715 2791 3145 233
Size: 681 801 1076 354 2745
Fragments arranged by size:
2745 1076 801 681 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 679 2236 3240 3373 3832 4041 679
Size: 1557 1004 133 459 209 2295
Fragments arranged by size:
2295 1557 1004 459 209 133
TatI w'GTAC_w
Cuts at: 494 1767 5172 494
Size: 1273 3405 979
Fragments arranged by size:
3405 1273 979
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 500 532 5174 5206 500
Size: 32 4642 32 951
Fragments arranged by size:
4642 951 32 32
Tth111I GACn'n_nGTC
Cuts at: 3454 3454
Size: 5657
XbaI T'CTAG_A
Cuts at: 2688 2688
Size: 5657
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3152 3152
Size: 5657
XhoI C'TCGA_G
Cuts at: 1235 1235
Size: 5657
XmaI C'CCGG_G
Cuts at: 2677 2677
Size: 5657
XmnI GAAnn'nnTTC
Cuts at: 2064 2576 2064
Size: 512 5145
ZraI GAC'GTC
Cuts at: 198 198
Size: 5657
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PciI
PflMI PfoI PmeI PpiI PsiI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AloI AscI AvrII BaeI
BmtI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I ClaI
CspCI DraIII FseI FspAI HpaI MfeI NdeI NheI
NotI NruI PacI PasI PmlI PpuMI PshAI PsrI
SanDI SgrAI SnaBI SrfI SspI SwaI