Restriction map for
pF1KA1003
(Circular) (Six-base) MAPSORT of: pF1KA1003.seq Check: 6549 from: 1 to: 5852
pF1KA1003 5852 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2178 2178
Size: 5852
AccI GT'mk_AC
Cuts at: 2890 2890
Size: 5852
Acc65I G'GTAC_C
Cuts at: 2868 2868
Size: 5852
AclI AA'CG_TT
Cuts at: 1516 5692 1516
Size: 4176 1676
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2701 3677 4109 4856 5124 2701
Size: 976 432 747 268 3429
Fragments arranged by size:
3429 976 747 432 268
AfeI AGC'GCT
Cuts at: 1423 1867 1423
Size: 444 5408
AflIII A'CryG_T
Cuts at: 1772 2237 2438 3204 4308 5285 1772
Size: 465 201 766 1104 977 2339
Fragments arranged by size:
2339 1104 977 766 465 201
AgeI A'CCGG_T
Cuts at: 4240 4240
Size: 5852
AleI CACnn'nnGTG
Cuts at: 1507 1507
Size: 5852
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1846 2584 1846
Size: 738 5114
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1084 1116 1084
Size: 32 5820
AlwNI CAG_nnn'CTG
Cuts at: 393 594 690 1226 2790 3157 4724 393
Size: 201 96 536 1564 367 1567 1521
Fragments arranged by size:
1567 1564 1521 536 367 201 96
ApaI G_GGCC'C
Cuts at: 2630 2797 2630
Size: 167 5685
ApaLI G'TGCA_C
Cuts at: 1962 4622 1962
Size: 2660 3192
ApoI r'AATT_y
Cuts at: 2856 5205 5461 2856
Size: 2349 256 3247
Fragments arranged by size:
3247 2349 256
AseI AT'TA_AT
Cuts at: 20 2060 20
Size: 2040 3812
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5852
AvaI C'yCGr_G
Cuts at: 530 556 887 969 1840 1924 2623 2755 2872
Size: 26 331 82 871 84 699 132 117
Cuts at: 2872 530
Size: 3510
Fragments arranged by size:
3510 871 699 331 132 117 84 82
26
BamHI G'GATC_C
Cuts at: 2877 2877
Size: 5852
BanI G'GyrC_C
Cuts at: 1356 1606 2868 3282 3530 3565 1356
Size: 250 1262 414 248 35 3643
Fragments arranged by size:
3643 1262 414 250 248 35
BanII G_rGCy'C
Cuts at: 415 848 940 1934 2219 2621 2630 2797 2866
Size: 433 92 994 285 402 9 167 69
Cuts at: 2866 3896 415
Size: 1030 2371
Fragments arranged by size:
2371 1030 994 433 402 285 167 92
69 9
BbeI G_GCGC'C
Cuts at: 1610 3286 3534 1610
Size: 1676 248 3928
Fragments arranged by size:
3928 1676 248
BbsI GAAGACnn'nnnn_
Cuts at: 1730 1730
Size: 5852
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1291 1325 2472 2506 1291
Size: 34 1147 34 4637
Fragments arranged by size:
4637 1147 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1509 1689 3750 4511 5852 1509
Size: 180 2061 761 1341 1509
Fragments arranged by size:
2061 1509 1341 761 180
BclI T'GATC_A
Cuts at: 3372 3372
Size: 5852
BglI GCCn_nnn'nGGC
Cuts at: 542 589 871 2012 2530 2625 542
Size: 47 282 1141 518 95 3769
Fragments arranged by size:
3769 1141 518 282 95 47
BglII A'GATC_T
Cuts at: 3367 5404 3367
Size: 2037 3815
BlpI GC'TnA_GC
Cuts at: 2964 2964
Size: 5852
Bme1580I G_kGCm'C
Cuts at: 1966 2033 2630 2797 3477 3570 4626 1966
Size: 67 597 167 680 93 1056 3192
Fragments arranged by size:
3192 1056 680 597 167 93 67
BmgBI CAC'GTC
Cuts at: 1911 1911
Size: 5852
BmrI ACTGGGnnnn_n'
Cuts at: 3064 3145 3480 5624 3064
Size: 81 335 2144 3292
Fragments arranged by size:
3292 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4219 4219
Size: 5852
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 102 403 1203 102
Size: 301 800 4751
Fragments arranged by size:
4751 800 301
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 832 1357 3028 3907 4399 4697 832
Size: 525 1671 879 492 298 1987
Fragments arranged by size:
1987 1671 879 525 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 699 2710 5556 699
Size: 2011 2846 995
Fragments arranged by size:
2846 2011 995
BsaAI yAC'GTr
Cuts at: 729 1368 2139 2238 2439 3205 729
Size: 639 771 99 201 766 3376
Fragments arranged by size:
3376 771 766 639 201 99
BsaBI GATnn'nnATC
Cuts at: 6 2494 2764 3366 5403 6
Size: 2488 270 602 2037 455
Fragments arranged by size:
2488 2037 602 455 270
BsaHI Gr'CG_yC
Cuts at: 1234 1607 2549 3283 3531 4233 5729 1234
Size: 373 942 734 248 702 1496 1357
Fragments arranged by size:
1496 1357 942 734 702 373 248
BsaWI w'CCGG_w
Cuts at: 214 932 3041 3562 4240 4514 4661 5072 214
Size: 718 2109 521 678 274 147 411 994
Fragments arranged by size:
2109 994 718 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 127 157 2380 2410 2428 2458 127
Size: 30 2223 30 18 30 3521
Fragments arranged by size:
3521 2223 30 30 30 18
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 405 856 1260 1981 2179 2551 2728 405
Size: 451 404 721 198 372 177 3529
Fragments arranged by size:
3529 721 451 404 372 198 177
BseYI C'CCAG_C
Cuts at: 408 791 1196 1690 2218 2790 3277 4035 4612
Size: 383 405 494 528 572 487 758 577
Cuts at: 4612 5108 408
Size: 496 1152
Fragments arranged by size:
1152 758 577 572 528 496 494 487
405 383
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 820 1495 2140 2791 820
Size: 675 645 651 3881
Fragments arranged by size:
3881 675 651 645
BsiEI CG_ry'CG
Cuts at: 104 3394 3440 4648 104
Size: 3290 46 1208 1308
Fragments arranged by size:
3290 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 848 1373 1966 2393 2446 2866 3644 3834 4626
Size: 525 593 427 53 420 778 190 792
Cuts at: 4626 848
Size: 2074
Fragments arranged by size:
2074 792 778 593 525 427 420 190
53
BsiWI C'GTAC_G
Cuts at: 1364 1364
Size: 5852
BsmI GAATG_Cn'
Cuts at: 1853 1853
Size: 5852
BsmBI CGTCTCn'nnnn_
Cuts at: 1024 1917 2104 2827 1024
Size: 893 187 723 4049
Fragments arranged by size:
4049 893 723 187
Bsp1286I G_dGCh'C
Cuts at: 415 848 940 1373 1934 1966 2033 2219 2393
Size: 433 92 433 561 32 67 186 174
Cuts at: 2393 2446 2621 2630 2797 2866 3477 3570 3644
Size: 53 175 9 167 69 611 93 74
Cuts at: 3644 3834 3896 4626 415
Size: 190 62 730 1641
Fragments arranged by size:
1641 730 611 561 433 433 190 186
175 174 167 93 92 74 69 67
62 53 32 9
BspEI T'CCGG_A
Cuts at: 5072 5072
Size: 5852
BspHI T'CATG_A
Cuts at: 5028 5028
Size: 5852
BspMI ACCTGCnnnn'nnnn_
Cuts at: 231 1946 2178 2813 2902 3116 3418 3799 231
Size: 1715 232 635 89 214 302 381 2284
Fragments arranged by size:
2284 1715 635 381 302 232 214 89
BsrBI CCG'CTC
Cuts at: 50 1083 1788 4144 4198 5682 5847 50
Size: 1033 705 2356 54 1484 165 55
Fragments arranged by size:
2356 1484 1033 705 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3764 3764
Size: 5852
BsrFI r'CCGG_y
Cuts at: 855 1593 3850 4031 4240 855
Size: 738 2257 181 209 2467
Fragments arranged by size:
2467 2257 738 209 181
BsrGI T'GTAC_A
Cuts at: 1775 2396 1775
Size: 621 5231
BssSI C'ACGA_G
Cuts at: 838 1300 1334 1471 1876 2107 4123 4481 838
Size: 462 34 137 405 231 2016 358 2209
Fragments arranged by size:
2209 2016 462 405 358 231 137 34
BstAPI GCAn_nnn'nTGC
Cuts at: 3229 3229
Size: 5852
BstBI TT'CG_AA
Cuts at: 4213 4213
Size: 5852
BstEII G'GTnAC_C
Cuts at: 156 210 928 156
Size: 54 718 5080
Fragments arranged by size:
5080 718 54
BstXI CCAn_nnnn'nTGG
Cuts at: 227 1509 227
Size: 1282 4570
BstYI r'GATC_y
Cuts at: 1 1844 2877 3344 3367 3702 3948 4960 5046
Size: 1843 1033 467 23 335 246 1012 86
Cuts at: 5046 5058 5404 1
Size: 12 346 449
Fragments arranged by size:
1843 1033 1012 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 866 1166 1187 1210 2010 2316 2843 107
Size: 759 300 21 23 800 306 527 3116
Fragments arranged by size:
3116 800 759 527 306 300 23 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2022 3802 3983 4146 2022
Size: 1780 181 163 3728
Fragments arranged by size:
3728 1780 181 163
BtsI GCAGTG_nn'
Cuts at: 981 1160 1483 1531 2031 2074 2578 3095 981
Size: 179 323 48 500 43 504 517 3738
Fragments arranged by size:
3738 517 504 500 323 179 48 43
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 710 745 710
Size: 35 5817
DraI TTT'AAA
Cuts at: 2851 2851
Size: 5852
DraIII CAC_nnn'GTG
Cuts at: 1881 1881
Size: 5852
DrdI GACnn_nn'nnGTC
Cuts at: 2092 2362 3558 4416 2092
Size: 270 1196 858 3528
Fragments arranged by size:
3528 1196 858 270
EaeI y'GGCC_r
Cuts at: 718 863 1559 1595 2201 2474 2667 3437 3611
Size: 145 696 36 606 273 193 770 174
Cuts at: 3611 4002 4029 5374 718
Size: 391 27 1345 1196
Fragments arranged by size:
1345 1196 770 696 606 391 273 193
174 145 36 27
EagI C'GGCC_G
Cuts at: 3437 3437
Size: 5852
EarI CTCTTCn'nnn_
Cuts at: 423 2149 3875 4085 423
Size: 1726 1726 210 2190
Fragments arranged by size:
2190 1726 1726 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1425 2758 4060 4370 4516 5662 1425
Size: 1333 1302 310 146 1146 1615
Fragments arranged by size:
1615 1333 1302 1146 310 146
EcoICRI GAG'CTC
Cuts at: 846 2864 846
Size: 2018 3834
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 102 403 1203 2701 3677 4109 4856 5124 102
Size: 301 800 1498 976 432 747 268 830
Fragments arranged by size:
1498 976 830 800 747 432 301 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1954 1954
Size: 5852
EcoO109I rG'GnC_Cy
Cuts at: 123 591 1554 1705 2284 2991 123
Size: 468 963 151 579 707 2984
Fragments arranged by size:
2984 963 707 579 468 151
EcoRI G'AATT_C
Cuts at: 2856 2856
Size: 5852
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 336 829 1747 3871 336
Size: 493 918 2124 2317
Fragments arranged by size:
2317 2124 918 493
FspI TGC'GCA
Cuts at: 649 3633 649
Size: 2984 2868
FspAI rTGC'GCAy
Cuts at: 649 649
Size: 5852
HaeII r_GCGC'y
Cuts at: 877 1425 1610 1869 3286 3534 4556 5535 877
Size: 548 185 259 1417 248 1022 979 1194
Fragments arranged by size:
1417 1194 1022 979 548 259 248 185
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 490 522 870 902 943 975 1483 1515 1951
Size: 32 348 32 41 32 508 32 436
Cuts at: 1951 1978 1983 2010 3694 3724 3726 3756 490
Size: 27 5 27 1684 30 2 30 2586
Fragments arranged by size:
2586 1684 508 436 348 41 32 32
32 32 30 30 27 27 5 2
HincII GTy'rAC
Cuts at: 768 2891 768
Size: 2123 3729
KasI G'GCGC_C
Cuts at: 1606 3282 3530 1606
Size: 1676 248 3928
Fragments arranged by size:
3928 1676 248
KpnI G_GTAC'C
Cuts at: 2872 2872
Size: 5852
MfeI C'AATT_G
Cuts at: 600 600
Size: 5852
MluI A'CGCG_T
Cuts at: 5285 5285
Size: 5852
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 369 1689 4523 4707 369
Size: 1320 2834 184 1514
Fragments arranged by size:
2834 1514 1320 184
MscI TGG'CCA
Cuts at: 865 1561 2476 3613 865
Size: 696 915 1137 3104
Fragments arranged by size:
3104 1137 915 696
MslI CAynn'nnrTG
Cuts at: 544 1165 1507 2566 3841 3968 544
Size: 621 342 1059 1275 127 2428
Fragments arranged by size:
2428 1275 1059 621 342 127
MspA1I CmG'CkG
Cuts at: 390 612 834 1189 1694 1750 1781 2679 2790
Size: 222 222 355 505 56 31 898 111
Cuts at: 2790 2963 3277 3637 4650 4895 390
Size: 173 314 360 1013 245 1347
Fragments arranged by size:
1347 1013 898 505 360 355 314 245
222 222 173 111 56 31
NaeI GCC'GGC
Cuts at: 1595 4033 1595
Size: 2438 3414
NarI GG'CG_CC
Cuts at: 1607 3283 3531 1607
Size: 1676 248 3928
Fragments arranged by size:
3928 1676 248
NcoI C'CATG_G
Cuts at: 107 1166 107
Size: 1059 4793
NgoMIV G'CCGG_C
Cuts at: 1593 4031 1593
Size: 2438 3414
NspI r_CATG'y
Cuts at: 1776 2571 2905 3405 4312 1776
Size: 795 334 500 907 3316
Fragments arranged by size:
3316 907 795 500 334
PasI CC'CwG_GG
Cuts at: 1632 1632
Size: 5852
PciI A'CATG_T
Cuts at: 1772 4308 1772
Size: 2536 3316
PflMI CCAn_nnn'nTGG
Cuts at: 690 2778 690
Size: 2088 3764
PfoI T'CCnGG_A
Cuts at: 380 1520 1542 2579 380
Size: 1140 22 1037 3653
Fragments arranged by size:
3653 1140 1037 22
PmeI GTTT'AAAC
Cuts at: 2851 2851
Size: 5852
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4991 5023 4991
Size: 32 5820
PpuMI rG'GwC_Cy
Cuts at: 123 1554 123
Size: 1431 4421
PspOMI G'GGCC_C
Cuts at: 2626 2793 2626
Size: 167 5685
PspXI vC'TCGA_Gb
Cuts at: 530 969 1924 530
Size: 439 955 4458
Fragments arranged by size:
4458 955 439
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 553 585 2419 2451 553
Size: 32 1834 32 3954
Fragments arranged by size:
3954 1834 32 32
PstI C_TGCA'G
Cuts at: 242 590 1285 2899 3584 242
Size: 348 695 1614 685 2510
Fragments arranged by size:
2510 1614 695 685 348
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5852
PvuII CAG'CTG
Cuts at: 834 1694 1781 2679 3277 3637 834
Size: 860 87 898 598 360 3049
Fragments arranged by size:
3049 898 860 598 360 87
RsrII CG'GwC_CG
Cuts at: 4047 4047
Size: 5852
SacI G_AGCT'C
Cuts at: 848 2866 848
Size: 2018 3834
SacII CC_GC'GG
Cuts at: 1190 1190
Size: 5852
SalI G'TCGA_C
Cuts at: 2889 2889
Size: 5852
SapI GCTCTTCn'nnn_
Cuts at: 2149 3875 4085 2149
Size: 1726 210 3916
Fragments arranged by size:
3916 1726 210
SbfI CC_TGCA'GG
Cuts at: 590 2899 590
Size: 2309 3543
ScaI AGT'ACT
Cuts at: 1819 5369 1819
Size: 3550 2302
SexAI A'CCwGG_T
Cuts at: 2357 2357
Size: 5852
SfcI C'TryA_G
Cuts at: 33 238 586 1281 2148 2895 3580 4573 4764
Size: 205 348 695 867 747 685 993 191
Cuts at: 4764 33
Size: 1121
Fragments arranged by size:
1121 993 867 747 695 685 348 205
191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2530 2530
Size: 5852
SfoI GGC'GCC
Cuts at: 1608 3284 3532 1608
Size: 1676 248 3928
Fragments arranged by size:
3928 1676 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5852
SmaI CCC'GGG
Cuts at: 2625 2874 2625
Size: 249 5603
SmlI C'TyrA_G
Cuts at: 530 847 969 1372 1924 3007 3922 4414 4676
Size: 317 122 403 552 1083 915 492 262
Cuts at: 4676 530
Size: 1706
Fragments arranged by size:
1706 1083 915 552 492 403 317 262
122
SnaBI TAC'GTA
Cuts at: 2139 2139
Size: 5852
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5852
SphI G_CATG'C
Cuts at: 2571 2905 3405 2571
Size: 334 500 5018
Fragments arranged by size:
5018 500 334
StuI AGG'CCT
Cuts at: 536 2524 536
Size: 1988 3864
StyI C'CwwG_G
Cuts at: 107 1166 1955 2986 3340 107
Size: 1059 789 1031 354 2619
Fragments arranged by size:
2619 1059 1031 789 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 817 1986 3435 3568 4027 4236 817
Size: 1169 1449 133 459 209 2433
Fragments arranged by size:
2433 1449 1169 459 209 133
TatI w'GTAC_w
Cuts at: 1658 1775 1817 1886 2396 5367 1658
Size: 117 42 69 510 2971 2143
Fragments arranged by size:
2971 2143 510 117 69 42
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 127 159 5369 5401 127
Size: 32 5210 32 578
Fragments arranged by size:
5210 578 32 32
Tth111I GACn'n_nGTC
Cuts at: 3649 3649
Size: 5852
XbaI T'CTAG_A
Cuts at: 2883 2883
Size: 5852
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2454 3347 2454
Size: 893 4959
XhoI C'TCGA_G
Cuts at: 530 969 1924 530
Size: 439 955 4458
Fragments arranged by size:
4458 955 439
XmaI C'CCGG_G
Cuts at: 2623 2872 2623
Size: 249 5603
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI FspAI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI
Enzymes that do not cut:
AatII AflII AhdI AscI AvrII BaeI BbvCI BfrBI
BmtI Bpu10I BssHII BstZ17I Bsu36I ClaI EcoRV FseI
HindIII HpaI NdeI NheI NotI NruI NsiI PacI
PmlI PshAI PsiI SanDI SgrAI SrfI SspI SwaI
XmnI ZraI