Restriction map for
pF1KA1008
(Circular) (Six-base) MAPSORT of: pF1KA1008.seq Check: 1695 from: 1 to: 5897
pF1KA1008 5897 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1149 1149
Size: 5897
AccI GT'mk_AC
Cuts at: 1451 2935 1451
Size: 1484 4413
Acc65I G'GTAC_C
Cuts at: 2913 2913
Size: 5897
AclI AA'CG_TT
Cuts at: 5737 5737
Size: 5897
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 683 1811 1897 1943 3722 4154 4901 5169 683
Size: 1128 86 46 1779 432 747 268 1411
Fragments arranged by size:
1779 1411 1128 747 432 268 86 46
AflII C'TTAA_G
Cuts at: 717 1006 717
Size: 289 5608
AflIII A'CryG_T
Cuts at: 3249 4353 5330 3249
Size: 1104 977 3816
Fragments arranged by size:
3816 1104 977
AgeI A'CCGG_T
Cuts at: 4285 4285
Size: 5897
AleI CACnn'nnGTG
Cuts at: 319 319
Size: 5897
AlwNI CAG_nnn'CTG
Cuts at: 284 3202 4769 284
Size: 2918 1567 1412
Fragments arranged by size:
2918 1567 1412
ApaLI G'TGCA_C
Cuts at: 4667 4667
Size: 5897
ApoI r'AATT_y
Cuts at: 1163 1239 1272 1512 1871 1995 2011 2048 2059
Size: 76 33 240 359 124 16 37 11
Cuts at: 2059 2117 2145 2469 2901 5250 5506 1163
Size: 58 28 324 432 2349 256 1554
Fragments arranged by size:
2349 1554 432 359 324 256 240 124
76 58 37 33 28 16 11
AseI AT'TA_AT
Cuts at: 20 1763 2605 20
Size: 1743 842 3312
Fragments arranged by size:
3312 1743 842
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5897
AvaI C'yCGr_G
Cuts at: 140 208 2917 140
Size: 68 2709 3120
Fragments arranged by size:
3120 2709 68
BamHI G'GATC_C
Cuts at: 2922 2922
Size: 5897
BanI G'GyrC_C
Cuts at: 2913 3327 3575 3610 2913
Size: 414 248 35 5200
Fragments arranged by size:
5200 414 248 35
BanII G_rGCy'C
Cuts at: 2911 3941 2911
Size: 1030 4867
BbeI G_GCGC'C
Cuts at: 3331 3579 3331
Size: 248 5649
BbsI GAAGACnn'nnnn_
Cuts at: 1131 1427 1131
Size: 296 5601
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5863
BciVI GTATCCnnnnn_n'
Cuts at: 3795 4556 5897 3795
Size: 761 1341 3795
Fragments arranged by size:
3795 1341 761
BclI T'GATC_A
Cuts at: 3417 3417
Size: 5897
BglII A'GATC_T
Cuts at: 1965 3412 5449 1965
Size: 1447 2037 2413
Fragments arranged by size:
2413 2037 1447
BlpI GC'TnA_GC
Cuts at: 1648 2316 3009 1648
Size: 668 693 4536
Fragments arranged by size:
4536 693 668
Bme1580I G_kGCm'C
Cuts at: 3522 3615 4671 3522
Size: 93 1056 4748
Fragments arranged by size:
4748 1056 93
BmgBI CAC'GTC
Cuts at: 1680 1680
Size: 5897
BmrI ACTGGGnnnn_n'
Cuts at: 2520 3109 3190 3525 5669 2520
Size: 589 81 335 2144 2748
Fragments arranged by size:
2748 2144 589 335 81
BmtI G_CTAG'C
Cuts at: 779 779
Size: 5897
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1175 2709 4264 1175
Size: 1534 1555 2808
Fragments arranged by size:
2808 1555 1534
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 363 369 1407 271
Size: 92 6 1038 4761
Fragments arranged by size:
4761 1038 92 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 97 3073 3952 4444 4742 97
Size: 2976 879 492 298 1252
Fragments arranged by size:
2976 1252 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1071 5601 1071
Size: 4530 1367
BsaAI yAC'GTr
Cuts at: 3250 3250
Size: 5897
BsaBI GATnn'nnATC
Cuts at: 6 160 2303 3411 5448 6
Size: 154 2143 1108 2037 455
Fragments arranged by size:
2143 2037 1108 455 154
BsaHI Gr'CG_yC
Cuts at: 3328 3576 4278 5774 3328
Size: 248 702 1496 3451
Fragments arranged by size:
3451 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3086 3607 4285 4559 4706 5117 3086
Size: 521 678 274 147 411 3866
Fragments arranged by size:
3866 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 3322 4080 4657 5153 3322
Size: 758 577 496 4066
Fragments arranged by size:
4066 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 313 313
Size: 5897
BsiEI CG_ry'CG
Cuts at: 104 3439 3485 4693 104
Size: 3335 46 1208 1308
Fragments arranged by size:
3335 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 176 2911 3689 3879 4671 176
Size: 2735 778 190 792 1402
Fragments arranged by size:
2735 1402 792 778 190
BsmI GAATG_Cn'
Cuts at: 1007 2595 2825 1007
Size: 1588 230 4079
Fragments arranged by size:
4079 1588 230
BsmBI CGTCTCn'nnnn_
Cuts at: 179 2327 179
Size: 2148 3749
Bsp1286I G_dGCh'C
Cuts at: 176 2911 3522 3615 3689 3879 3941 4671 176
Size: 2735 611 93 74 190 62 730 1402
Fragments arranged by size:
2735 1402 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 5117 5117
Size: 5897
BspHI T'CATG_A
Cuts at: 2052 5073 2052
Size: 3021 2876
BspMI ACCTGCnnnn'nnnn_
Cuts at: 188 1149 2947 3161 3463 3844 188
Size: 961 1798 214 302 381 2241
Fragments arranged by size:
2241 1798 961 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4189 4243 5727 5892 50
Size: 4139 54 1484 165 55
Fragments arranged by size:
4139 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3809 3809
Size: 5897
BsrFI r'CCGG_y
Cuts at: 243 3895 4076 4285 243
Size: 3652 181 209 1855
Fragments arranged by size:
3652 1855 209 181
BssSI C'ACGA_G
Cuts at: 235 4168 4526 235
Size: 3933 358 1606
Fragments arranged by size:
3933 1606 358
BstAPI GCAn_nnn'nTGC
Cuts at: 180 284 303 3274 180
Size: 104 19 2971 2803
Fragments arranged by size:
2971 2803 104 19
BstBI TT'CG_AA
Cuts at: 2082 4258 2082
Size: 2176 3721
BstXI CCAn_nnnn'nTGG
Cuts at: 2178 2178
Size: 5897
BstYI r'GATC_y
Cuts at: 1 1965 2783 2922 3389 3412 3747 3993 5005
Size: 1964 818 139 467 23 335 246 1012
Cuts at: 5005 5091 5103 5449 1
Size: 86 12 346 449
Fragments arranged by size:
1964 1012 818 467 449 346 335 246
139 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1426 1426
Size: 5897
BtgI C'CryG_G
Cuts at: 1848 2000 1848
Size: 152 5745
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3847 4028 4191 3847
Size: 181 163 5553
Fragments arranged by size:
5553 181 163
BtsI GCAGTG_nn'
Cuts at: 3140 3140
Size: 5897
DraI TTT'AAA
Cuts at: 866 2896 866
Size: 2030 3867
DraIII CAC_nnn'GTG
Cuts at: 1266 1266
Size: 5897
DrdI GACnn_nn'nnGTC
Cuts at: 3603 4461 3603
Size: 858 5039
EaeI y'GGCC_r
Cuts at: 3482 3656 4047 4074 5419 3482
Size: 174 391 27 1345 3960
Fragments arranged by size:
3960 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3482 3482
Size: 5897
EarI CTCTTCn'nnn_
Cuts at: 983 1587 3920 4130 983
Size: 604 2333 210 2750
Fragments arranged by size:
2750 2333 604 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4105 4415 4561 5707 4105
Size: 310 146 1146 4295
Fragments arranged by size:
4295 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2909 2909
Size: 5897
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 363 369 683 1407 1811 1897 1943 3722
Size: 92 6 314 724 404 86 46 1779
Cuts at: 3722 4154 4901 5169 271
Size: 432 747 268 999
Fragments arranged by size:
1779 999 747 724 432 404 314 268
92 86 46 6
EcoO109I rG'GnC_Cy
Cuts at: 267 359 3036 267
Size: 92 2677 3128
Fragments arranged by size:
3128 2677 92
EcoRI G'AATT_C
Cuts at: 1163 2901 1163
Size: 1738 4159
EcoRV GAT'ATC
Cuts at: 1249 1249
Size: 5897
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 144 3916 144
Size: 3772 2125
FseI GG_CCGG'CC
Cuts at: 247 247
Size: 5897
FspI TGC'GCA
Cuts at: 216 3678 216
Size: 3462 2435
HaeII r_GCGC'y
Cuts at: 3331 3579 4601 5580 3331
Size: 248 1022 979 3648
Fragments arranged by size:
3648 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 176 208 1567 1599 3739 3769 3771 3801 176
Size: 32 1359 32 2140 30 2 30 2272
Fragments arranged by size:
2272 2140 1359 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2429 2936 2429
Size: 507 5390
HindIII A'AGCT_T
Cuts at: 2443 2881 2443
Size: 438 5459
KasI G'GCGC_C
Cuts at: 3327 3575 3327
Size: 248 5649
KpnI G_GTAC'C
Cuts at: 2917 2917
Size: 5897
MluI A'CGCG_T
Cuts at: 5330 5330
Size: 5897
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1266 4568 4752 1266
Size: 3302 184 2411
Fragments arranged by size:
3302 2411 184
MscI TGG'CCA
Cuts at: 3658 3658
Size: 5897
MslI CAynn'nnrTG
Cuts at: 319 1461 1698 2309 3886 4013 319
Size: 1142 237 611 1577 127 2203
Fragments arranged by size:
2203 1577 1142 611 237 127
MspA1I CmG'CkG
Cuts at: 522 3008 3322 3682 4695 4940 522
Size: 2486 314 360 1013 245 1479
Fragments arranged by size:
2486 1479 1013 360 314 245
NaeI GCC'GGC
Cuts at: 245 4078 245
Size: 3833 2064
NarI GG'CG_CC
Cuts at: 3328 3576 3328
Size: 248 5649
NcoI C'CATG_G
Cuts at: 2000 2000
Size: 5897
NdeI CA'TA_TG
Cuts at: 1785 1785
Size: 5897
NgoMIV G'CCGG_C
Cuts at: 243 4076 243
Size: 3833 2064
NheI G'CTAG_C
Cuts at: 775 775
Size: 5897
NspI r_CATG'y
Cuts at: 1095 2074 2950 3450 4357 1095
Size: 979 876 500 907 2635
Fragments arranged by size:
2635 979 907 876 500
PciI A'CATG_T
Cuts at: 4353 4353
Size: 5897
PflMI CCAn_nnn'nTGG
Cuts at: 1087 2617 1087
Size: 1530 4367
PmeI GTTT'AAAC
Cuts at: 2896 2896
Size: 5897
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5036 5068 5036
Size: 32 5865
PpuMI rG'GwC_Cy
Cuts at: 267 359 267
Size: 92 5805
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2795 2827 2795
Size: 32 5865
PstI C_TGCA'G
Cuts at: 1973 2944 3629 1973
Size: 971 685 4241
Fragments arranged by size:
4241 971 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5897
PvuII CAG'CTG
Cuts at: 522 3322 3682 522
Size: 2800 360 2737
Fragments arranged by size:
2800 2737 360
RsrII CG'GwC_CG
Cuts at: 4092 4092
Size: 5897
SacI G_AGCT'C
Cuts at: 2911 2911
Size: 5897
SalI G'TCGA_C
Cuts at: 2934 2934
Size: 5897
SapI GCTCTTCn'nnn_
Cuts at: 3920 4130 3920
Size: 210 5687
SbfI CC_TGCA'GG
Cuts at: 2944 2944
Size: 5897
ScaI AGT'ACT
Cuts at: 5414 5414
Size: 5897
SfcI C'TryA_G
Cuts at: 33 727 800 1040 1961 1969 2940 3625 4618
Size: 694 73 240 921 8 971 685 993
Cuts at: 4618 4809 33
Size: 191 1121
Fragments arranged by size:
1121 993 971 921 694 685 240 191
73 8
SfoI GGC'GCC
Cuts at: 3329 3577 3329
Size: 248 5649
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5897
SmaI CCC'GGG
Cuts at: 142 210 2919 142
Size: 68 2709 3120
Fragments arranged by size:
3120 2709 68
SmlI C'TyrA_G
Cuts at: 112 717 1006 3052 3967 4459 4721 112
Size: 605 289 2046 915 492 262 1288
Fragments arranged by size:
2046 1288 915 605 492 289 262
SpeI A'CTAG_T
Cuts at: 67 1840 67
Size: 1773 4124
SphI G_CATG'C
Cuts at: 1095 2950 3450 1095
Size: 1855 500 3542
Fragments arranged by size:
3542 1855 500
SspI AAT'ATT
Cuts at: 698 2031 2243 698
Size: 1333 212 4352
Fragments arranged by size:
4352 1333 212
StyI C'CwwG_G
Cuts at: 1571 1976 2000 3031 3385 1571
Size: 405 24 1031 354 4083
Fragments arranged by size:
4083 1031 405 354 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2364 3480 3613 4072 4281 2364
Size: 1116 133 459 209 3980
Fragments arranged by size:
3980 1116 459 209 133
TatI w'GTAC_w
Cuts at: 1465 2273 2413 5412 1465
Size: 808 140 2999 1950
Fragments arranged by size:
2999 1950 808 140
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1250 1282 1589 1621 5414 5446 1250
Size: 32 307 32 3793 32 1701
Fragments arranged by size:
3793 1701 307 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3694 3694
Size: 5897
XbaI T'CTAG_A
Cuts at: 2928 2928
Size: 5897
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1382 3392 1382
Size: 2010 3887
XmaI C'CCGG_G
Cuts at: 140 208 2917 140
Size: 68 2709 3120
Fragments arranged by size:
3120 2709 68
XmnI GAAnn'nnTTC
Cuts at: 1809 1809
Size: 5897
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FseI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NspI PciI PflMI PmeI PpiI
PpuMI PsrI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AlfI AloI ApaI AscI AvrII
BaeI BbvCI BfrBI BglI Bpu10I BsaXI BseRI BsiWI
BsrGI BssHII BstEII BstZ17I ClaI CspCI EcoNI FspAI
HpaI MfeI NotI NruI NsiI PacI PasI PfoI
PmlI PshAI PsiI PspOMI PspXI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI