Restriction map for
pF1KA1012
(Circular) (Six-base) MAPSORT of: pF1KA1012.seq Check: 9745 from: 1 to: 7418
pF1KA1012 7418 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AccI GT'mk_AC
Cuts at: 3113 4456 3113
Size: 1343 6075
Acc65I G'GTAC_C
Cuts at: 4434 4434
Size: 7418
AclI AA'CG_TT
Cuts at: 7258 7258
Size: 7418
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 484 991 2546 5243 5675 6028 6690 484
Size: 507 1555 2697 432 353 662 1212
Fragments arranged by size:
2697 1555 1212 662 507 432 353
AflII C'TTAA_G
Cuts at: 235 235
Size: 7418
AflIII A'CryG_T
Cuts at: 4770 6570 6851 4770
Size: 1800 281 5337
Fragments arranged by size:
5337 1800 281
AleI CACnn'nnGTG
Cuts at: 635 635
Size: 7418
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1764 1764
Size: 7418
AlwNI CAG_nnn'CTG
Cuts at: 1513 1672 3031 4723 6161 1513
Size: 159 1359 1692 1438 2770
Fragments arranged by size:
2770 1692 1438 1359 159
ApaLI G'TGCA_C
Cuts at: 1721 6256 1721
Size: 4535 2883
ApoI r'AATT_y
Cuts at: 306 526 623 921 1148 2744 2914 3463 3970
Size: 220 97 298 227 1596 170 549 507
Cuts at: 3970 4377 4422 6771 7027 306
Size: 407 45 2349 256 697
Fragments arranged by size:
2349 1596 697 549 507 407 298 256
227 220 170 97 45
AseI AT'TA_AT
Cuts at: 20 791 1316 1916 1994 2549 20
Size: 771 525 600 78 555 4889
Fragments arranged by size:
4889 771 600 555 525 78
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7418
AvaI C'yCGr_G
Cuts at: 4438 4438
Size: 7418
AvrII C'CTAG_G
Cuts at: 1537 4321 1537
Size: 2784 4634
BamHI G'GATC_C
Cuts at: 4443 4443
Size: 7418
BanI G'GyrC_C
Cuts at: 1489 1931 4434 4848 5096 5131 1489
Size: 442 2503 414 248 35 3776
Fragments arranged by size:
3776 2503 442 414 248 35
BanII G_rGCy'C
Cuts at: 999 2653 4432 5462 999
Size: 1654 1779 1030 2955
Fragments arranged by size:
2955 1779 1654 1030
BbeI G_GCGC'C
Cuts at: 1935 4852 5100 1935
Size: 2917 248 4253
Fragments arranged by size:
4253 2917 248
BbsI GAAGACnn'nnnn_
Cuts at: 3863 4039 3863
Size: 176 7242
BbvCI CC'TCA_GC
Cuts at: 217 347 3024 217
Size: 130 2677 4611
Fragments arranged by size:
4611 2677 130
BciVI GTATCCnnnnn_n'
Cuts at: 1358 1557 5316 6372 7418 1358
Size: 199 3759 1056 1046 1358
Fragments arranged by size:
3759 1358 1056 1046 199
BclI T'GATC_A
Cuts at: 1137 1464 2202 4394 4938 1137
Size: 327 738 2192 544 3617
Fragments arranged by size:
3617 2192 738 544 327
BfrBI ATG'CAT
Cuts at: 1789 1789
Size: 7418
BglII A'GATC_T
Cuts at: 1063 4933 6970 1063
Size: 3870 2037 1511
Fragments arranged by size:
3870 2037 1511
BlpI GC'TnA_GC
Cuts at: 4530 4530
Size: 7418
Bme1580I G_kGCm'C
Cuts at: 1725 5043 5136 6260 1725
Size: 3318 93 1124 2883
Fragments arranged by size:
3318 2883 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 108 4630 4711 5046 7190 108
Size: 4522 81 335 2144 336
Fragments arranged by size:
4522 2144 336 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1274 5785 1274
Size: 4511 2907
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 374 838 374
Size: 464 6954
Bpu10I CC'TnA_GC
Cuts at: 217 347 3024 217
Size: 130 2677 4611
Fragments arranged by size:
4611 2677 130
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2266 2730 3987 4594 5473 6187 6485 2266
Size: 464 1257 607 879 714 298 3199
Fragments arranged by size:
3199 1257 879 714 607 464 298
BsaI GGTCTCn'nnnn_
Cuts at: 1901 7122 1901
Size: 5221 2197
BsaAI yAC'GTr
Cuts at: 297 3217 4771 297
Size: 2920 1554 2944
Fragments arranged by size:
2944 2920 1554
BsaBI GATnn'nnATC
Cuts at: 6 1709 4399 4932 6969 6
Size: 1703 2690 533 2037 455
Fragments arranged by size:
2690 2037 1703 533 455
BsaHI Gr'CG_yC
Cuts at: 1932 4849 5097 5799 7295 1932
Size: 2917 248 702 1496 2055
Fragments arranged by size:
2917 2055 1496 702 248
BsaWI w'CCGG_w
Cuts at: 362 4607 5128 5806 6217 6364 6638 362
Size: 4245 521 678 411 147 274 1142
Fragments arranged by size:
4245 1142 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2513 2543 3179 3209 2513
Size: 30 636 30 6722
Fragments arranged by size:
6722 636 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 3413 3413
Size: 7418
BseYI C'CCAG_C
Cuts at: 338 4843 5601 6266 6674 338
Size: 4505 758 665 408 1082
Fragments arranged by size:
4505 1082 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 198 728 1426 3217 3713 151
Size: 47 530 698 1791 496 3856
Fragments arranged by size:
3856 1791 698 530 496 47
BsiEI CG_ry'CG
Cuts at: 104 4960 5006 6236 104
Size: 4856 46 1230 1286
Fragments arranged by size:
4856 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 999 1536 1725 2679 4432 5210 5400 6260 999
Size: 537 189 954 1753 778 190 860 2157
Fragments arranged by size:
2157 1753 954 860 778 537 190 189
BsmBI CGTCTCn'nnnn_
Cuts at: 211 211
Size: 7418
Bsp1286I G_dGCh'C
Cuts at: 999 1536 1725 2653 2679 4432 5043 5136 5210
Size: 537 189 928 26 1753 611 93 74
Cuts at: 5210 5400 5462 6260 999
Size: 190 62 798 2157
Fragments arranged by size:
2157 1753 928 798 611 537 190 189
93 74 62 26
BspEI T'CCGG_A
Cuts at: 362 362
Size: 7418
BspHI T'CATG_A
Cuts at: 522 5850 522
Size: 5328 2090
BspMI ACCTGCnnnn'nnnn_
Cuts at: 4468 4682 4984 5365 4468
Size: 214 302 381 6521
Fragments arranged by size:
6521 381 302 214
BsrBI CCG'CTC
Cuts at: 50 195 5710 5764 7248 7413 50
Size: 145 5515 54 1484 165 55
Fragments arranged by size:
5515 1484 165 145 55 54
BsrDI GCAATG_nn'
Cuts at: 1478 1746 2431 5330 1478
Size: 268 685 2899 3566
Fragments arranged by size:
3566 2899 685 268
BsrFI r'CCGG_y
Cuts at: 5416 5597 5416
Size: 181 7237
BsrGI T'GTAC_A
Cuts at: 120 3082 120
Size: 2962 4456
BssSI C'ACGA_G
Cuts at: 5689 6397 5689
Size: 708 6710
BstAPI GCAn_nnn'nTGC
Cuts at: 1489 1513 4795 1489
Size: 24 3282 4112
Fragments arranged by size:
4112 3282 24
BstBI TT'CG_AA
Cuts at: 1716 5779 1716
Size: 4063 3355
BstXI CCAn_nnnn'nTGG
Cuts at: 839 839
Size: 7418
BstYI r'GATC_y
Cuts at: 1 276 834 1063 2817 4443 4910 4933 5268
Size: 275 558 229 1754 1626 467 23 335
Cuts at: 5268 5514 5820 5832 5918 6970 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1754 1626 1052 558 467 449 335 306
275 246 229 86 23 12
BtgI C'CryG_G
Cuts at: 107 469 1772 4200 107
Size: 362 1303 2428 3325
Fragments arranged by size:
3325 2428 1303 362
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5368 5549 5712 5368
Size: 181 163 7074
Fragments arranged by size:
7074 181 163
BtsI GCAGTG_nn'
Cuts at: 1514 2239 3314 3813 4661 1514
Size: 725 1075 499 848 4271
Fragments arranged by size:
4271 1075 848 725 499
ClaI AT'CG_AT
Cuts at: 1815 1815
Size: 7418
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 992 1027 2357 2392 992
Size: 35 1330 35 6018
Fragments arranged by size:
6018 1330 35 35
DraI TTT'AAA
Cuts at: 1646 3344 4417 1646
Size: 1698 1073 4647
Fragments arranged by size:
4647 1698 1073
DraIII CAC_nnn'GTG
Cuts at: 3474 3474
Size: 7418
DrdI GACnn_nn'nnGTC
Cuts at: 154 2427 5124 6468 154
Size: 2273 2697 1344 1104
Fragments arranged by size:
2697 2273 1344 1104
EaeI y'GGCC_r
Cuts at: 2145 3611 5003 5177 5568 5595 6940 2145
Size: 1466 1392 174 391 27 1345 2623
Fragments arranged by size:
2623 1466 1392 1345 391 174 27
EagI C'GGCC_G
Cuts at: 5003 5003
Size: 7418
EarI CTCTTCn'nnn_
Cuts at: 2684 2771 5441 5651 2684
Size: 87 2670 210 4451
Fragments arranged by size:
4451 2670 210 87
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1340 5626 6368 6514 7228 1340
Size: 4286 742 146 714 1530
Fragments arranged by size:
4286 1530 742 714 146
EcoICRI GAG'CTC
Cuts at: 997 4430 997
Size: 3433 3985
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 374 484 838 991 2546 5243 5675 6028 6690
Size: 110 354 153 1555 2697 432 353 662
Cuts at: 6690 374
Size: 1102
Fragments arranged by size:
2697 1555 1102 662 432 354 353 153
110
EcoNI CCTnn'n_nnAGG
Cuts at: 404 404
Size: 7418
EcoO109I rG'GnC_Cy
Cuts at: 1182 2752 4557 1182
Size: 1570 1805 4043
Fragments arranged by size:
4043 1805 1570
EcoRI G'AATT_C
Cuts at: 623 921 1148 3463 4377 4422 623
Size: 298 227 2315 914 45 3619
Fragments arranged by size:
3619 2315 914 298 227 45
EcoRV GAT'ATC
Cuts at: 1590 3648 1590
Size: 2058 5360
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1085 1474 1492 2428 3594 3734 5437 1085
Size: 389 18 936 1166 140 1703 3066
Fragments arranged by size:
3066 1703 1166 936 389 140 18
FspI TGC'GCA
Cuts at: 5199 5199
Size: 7418
HaeII r_GCGC'y
Cuts at: 1935 4852 5100 6330 7101 1935
Size: 2917 248 1230 771 2252
Fragments arranged by size:
2917 2252 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 819 834 851 866 2206 2238 2567 2599 2770
Size: 15 17 15 1340 32 329 32 171
Cuts at: 2770 2802 3058 3090 4251 4283 5260 5290 5292
Size: 32 256 32 1161 32 977 30 2
Cuts at: 5292 5322 819
Size: 30 2915
Fragments arranged by size:
2915 1340 1161 977 329 256 171 32
32 32 32 32 30 30 17 15
15 2
HincII GTy'rAC
Cuts at: 1694 2271 2420 3139 3773 3886 4457 1694
Size: 577 149 719 634 113 571 4655
Fragments arranged by size:
4655 719 634 577 571 149 113
HindIII A'AGCT_T
Cuts at: 367 367
Size: 7418
HpaI GTT'AAC
Cuts at: 2271 2271
Size: 7418
KasI G'GCGC_C
Cuts at: 1931 4848 5096 1931
Size: 2917 248 4253
Fragments arranged by size:
4253 2917 248
KpnI G_GTAC'C
Cuts at: 4438 4438
Size: 7418
MfeI C'AATT_G
Cuts at: 4125 4125
Size: 7418
MluI A'CGCG_T
Cuts at: 6851 6851
Size: 7418
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 992 2828 6177 6361 992
Size: 1836 3349 184 2049
Fragments arranged by size:
3349 2049 1836 184
MscI TGG'CCA
Cuts at: 2147 5179 2147
Size: 3032 4386
MslI CAynn'nnrTG
Cuts at: 635 4212 4298 5407 5534 635
Size: 3577 86 1109 127 2519
Fragments arranged by size:
3577 2519 1109 127 86
MspA1I CmG'CkG
Cuts at: 1486 1956 2073 2164 3201 4275 4529 4843 5203
Size: 470 117 91 1037 1074 254 314 360
Cuts at: 5203 5987 6232 1486
Size: 784 245 2672
Fragments arranged by size:
2672 1074 1037 784 470 360 314 254
245 117 91
NaeI GCC'GGC
Cuts at: 5599 5599
Size: 7418
NarI GG'CG_CC
Cuts at: 1932 4849 5097 1932
Size: 2917 248 4253
Fragments arranged by size:
4253 2917 248
NcoI C'CATG_G
Cuts at: 107 469 1772 4200 107
Size: 362 1303 2428 3325
Fragments arranged by size:
3325 2428 1303 362
NdeI CA'TA_TG
Cuts at: 885 1796 2907 885
Size: 911 1111 5396
Fragments arranged by size:
5396 1111 911
NgoMIV G'CCGG_C
Cuts at: 5597 5597
Size: 7418
NsiI A_TGCA'T
Cuts at: 1791 1791
Size: 7418
NspI r_CATG'y
Cuts at: 551 1124 1846 4286 4471 4971 6574 551
Size: 573 722 2440 185 500 1603 1395
Fragments arranged by size:
2440 1603 1395 722 573 500 185
PacI TTA_AT'TAA
Cuts at: 2549 2549
Size: 7418
PciI A'CATG_T
Cuts at: 6570 6570
Size: 7418
PflMI CCAn_nnn'nTGG
Cuts at: 2167 2944 2167
Size: 777 6641
PfoI T'CCnGG_A
Cuts at: 143 2682 143
Size: 2539 4879
PmeI GTTT'AAAC
Cuts at: 4417 4417
Size: 7418
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2311 2343 5864 5896 2311
Size: 32 3521 32 3833
Fragments arranged by size:
3833 3521 32 32
PpuMI rG'GwC_Cy
Cuts at: 2752 2752
Size: 7418
PsiI TTA'TAA
Cuts at: 1756 2630 4307 1756
Size: 874 1677 4867
Fragments arranged by size:
4867 1677 874
PstI C_TGCA'G
Cuts at: 405 1677 3367 4274 4465 5150 405
Size: 1272 1690 907 191 685 2673
Fragments arranged by size:
2673 1690 1272 907 685 191
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7418
PvuII CAG'CTG
Cuts at: 1486 1956 2073 3201 4275 4843 5203 1486
Size: 470 117 1128 1074 568 360 3701
Fragments arranged by size:
3701 1128 1074 568 470 360 117
RsrII CG'GwC_CG
Cuts at: 5613 5613
Size: 7418
SacI G_AGCT'C
Cuts at: 999 4432 999
Size: 3433 3985
SalI G'TCGA_C
Cuts at: 4455 4455
Size: 7418
SapI GCTCTTCn'nnn_
Cuts at: 2684 5441 5651 2684
Size: 2757 210 4451
Fragments arranged by size:
4451 2757 210
SbfI CC_TGCA'GG
Cuts at: 4465 4465
Size: 7418
ScaI AGT'ACT
Cuts at: 6935 6935
Size: 7418
SfcI C'TryA_G
Cuts at: 33 401 1673 2873 3110 3363 3908 4270 4461
Size: 368 1272 1200 237 253 545 362 191
Cuts at: 4461 5146 6114 6305 33
Size: 685 968 191 1146
Fragments arranged by size:
1272 1200 1146 968 685 545 368 362
253 237 191 191
SfoI GGC'GCC
Cuts at: 1933 4850 5098 1933
Size: 2917 248 4253
Fragments arranged by size:
4253 2917 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7418
SmaI CCC'GGG
Cuts at: 4440 4440
Size: 7418
SmlI C'TyrA_G
Cuts at: 235 2245 2709 4002 4573 5488 6202 6464 235
Size: 2010 464 1293 571 915 714 262 1189
Fragments arranged by size:
2010 1293 1189 915 714 571 464 262
SpeI A'CTAG_T
Cuts at: 67 2493 3442 67
Size: 2426 949 4043
Fragments arranged by size:
4043 2426 949
SphI G_CATG'C
Cuts at: 1846 4286 4471 4971 1846
Size: 2440 185 500 4293
Fragments arranged by size:
4293 2440 500 185
SspI AAT'ATT
Cuts at: 1663 2704 3305 3825 1663
Size: 1041 601 520 5256
Fragments arranged by size:
5256 1041 601 520
StyI C'CwwG_G
Cuts at: 107 469 838 1493 1537 1772 4200 4311 4321
Size: 362 369 655 44 235 2428 111 10
Cuts at: 4321 4552 4906 107
Size: 231 354 2619
Fragments arranged by size:
2619 2428 655 369 362 354 235 231
111 44 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 352 1016 3326 5001 5134 5593 5802 352
Size: 664 2310 1675 133 459 209 1968
Fragments arranged by size:
2310 1968 1675 664 459 209 133
TatI w'GTAC_w
Cuts at: 120 687 901 3082 6933 120
Size: 567 214 2181 3851 605
Fragments arranged by size:
3851 2181 605 567 214
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3179 3211 4181 4213 6935 6967 3179
Size: 32 970 32 2722 32 3630
Fragments arranged by size:
3630 2722 970 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 5215 5215
Size: 7418
XbaI T'CTAG_A
Cuts at: 4449 4449
Size: 7418
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4913 4913
Size: 7418
XmaI C'CCGG_G
Cuts at: 4438 4438
Size: 7418
XmnI GAAnn'nnTTC
Cuts at: 494 2703 3372 3973 494
Size: 2209 669 601 3939
Fragments arranged by size:
3939 2209 669 601
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI
NspI PacI PciI PflMI PfoI PmeI PpiI PpuMI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AloI ApaI AscI
BaeI BcgI BglI BmgBI BmtI BsiWI BsmI BssHII
BstEII BstZ17I Bsu36I FseI FspAI NheI NotI NruI
PasI PmlI PshAI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
ZraI