Restriction map for
pF1KA1016
(Circular) (Six-base) MAPSORT of: pF1KA1016.seq Check: 7444 from: 1 to: 5297
pF1KA1016 5297 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AccI GT'mk_AC
Cuts at: 1899 2335 1899
Size: 436 4861
Acc65I G'GTAC_C
Cuts at: 2313 2313
Size: 5297
AclI AA'CG_TT
Cuts at: 5137 5137
Size: 5297
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1768 3122 3554 4301 4569 1768
Size: 1354 432 747 268 2496
Fragments arranged by size:
2496 1354 747 432 268
AflIII A'CryG_T
Cuts at: 2649 3753 4730 2649
Size: 1104 977 3216
Fragments arranged by size:
3216 1104 977
AgeI A'CCGG_T
Cuts at: 3685 3685
Size: 5297
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 856 948 856
Size: 92 5205
AlwNI CAG_nnn'CTG
Cuts at: 540 2089 2602 4169 540
Size: 1549 513 1567 1668
Fragments arranged by size:
1668 1567 1549 513
ApaLI G'TGCA_C
Cuts at: 1399 4067 1399
Size: 2668 2629
ApoI r'AATT_y
Cuts at: 361 476 1231 1243 1336 1782 2301 4650 4906
Size: 115 755 12 93 446 519 2349 256
Cuts at: 4906 361
Size: 752
Fragments arranged by size:
2349 755 752 519 446 256 115 93
12
AseI AT'TA_AT
Cuts at: 20 20
Size: 5297
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5297
AvaI C'yCGr_G
Cuts at: 382 2167 2317 382
Size: 1785 150 3362
Fragments arranged by size:
3362 1785 150
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 483 516 483
Size: 33 5264
BamHI G'GATC_C
Cuts at: 2322 2322
Size: 5297
BanI G'GyrC_C
Cuts at: 226 2313 2727 2975 3010 226
Size: 2087 414 248 35 2513
Fragments arranged by size:
2513 2087 414 248 35
BanII G_rGCy'C
Cuts at: 2311 3341 2311
Size: 1030 4267
BbeI G_GCGC'C
Cuts at: 230 2731 2979 230
Size: 2501 248 2548
Fragments arranged by size:
2548 2501 248
BbsI GAAGACnn'nnnn_
Cuts at: 1139 1370 1913 1139
Size: 231 543 4523
Fragments arranged by size:
4523 543 231
BciVI GTATCCnnnnn_n'
Cuts at: 3195 3956 5297 3195
Size: 761 1341 3195
Fragments arranged by size:
3195 1341 761
BclI T'GATC_A
Cuts at: 2817 2817
Size: 5297
BglII A'GATC_T
Cuts at: 801 2812 4849 801
Size: 2011 2037 1249
Fragments arranged by size:
2037 2011 1249
BlpI GC'TnA_GC
Cuts at: 2409 2409
Size: 5297
Bme1580I G_kGCm'C
Cuts at: 1403 2922 3015 4071 1403
Size: 1519 93 1056 2629
Fragments arranged by size:
2629 1519 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2509 2590 2925 5069 2509
Size: 81 335 2144 2737
Fragments arranged by size:
2737 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3664 3664
Size: 5297
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 905 1633 2190 316
Size: 589 728 557 3423
Fragments arranged by size:
3423 728 589 557
Bpu10I CC'TnA_GC
Cuts at: 340 1213 340
Size: 873 4424
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 328 576 916 2473 3352 3844 4142 328
Size: 248 340 1557 879 492 298 1483
Fragments arranged by size:
1557 1483 879 492 340 298 248
BsaI GGTCTCn'nnnn_
Cuts at: 1292 1330 5001 1292
Size: 38 3671 1588
Fragments arranged by size:
3671 1588 38
BsaAI yAC'GTr
Cuts at: 1047 2650 1047
Size: 1603 3694
BsaBI GATnn'nnATC
Cuts at: 6 2811 4848 6
Size: 2805 2037 455
Fragments arranged by size:
2805 2037 455
BsaHI Gr'CG_yC
Cuts at: 115 227 2728 2976 3678 5174 115
Size: 112 2501 248 702 1496 238
Fragments arranged by size:
2501 1496 702 248 238 112
BsaWI w'CCGG_w
Cuts at: 1251 1623 1814 2486 3007 3685 3959 4106 4517
Size: 372 191 672 521 678 274 147 411
Cuts at: 4517 1251
Size: 2031
Fragments arranged by size:
2031 678 672 521 411 372 274 191
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1186 1216 1186
Size: 30 5267
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 325 908 325
Size: 583 4714
BseYI C'CCAG_C
Cuts at: 719 2722 3480 4057 4553 719
Size: 2003 758 577 496 1463
Fragments arranged by size:
2003 1463 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 427 1574 151
Size: 276 1147 3874
Fragments arranged by size:
3874 1147 276
BsiEI CG_ry'CG
Cuts at: 104 1975 2839 2885 4093 104
Size: 1871 864 46 1208 1308
Fragments arranged by size:
1871 1308 1208 864 46
BsiHKAI G_wGCw'C
Cuts at: 845 1403 2311 3089 3279 4071 845
Size: 558 908 778 190 792 2071
Fragments arranged by size:
2071 908 792 778 558 190
BsmI GAATG_Cn'
Cuts at: 2283 2283
Size: 5297
Bsp1286I G_dGCh'C
Cuts at: 845 1403 2311 2922 3015 3089 3279 3341 4071
Size: 558 908 611 93 74 190 62 730
Cuts at: 4071 845
Size: 2071
Fragments arranged by size:
2071 908 730 611 558 190 93 74
62
BspEI T'CCGG_A
Cuts at: 1814 4517 1814
Size: 2703 2594
BspHI T'CATG_A
Cuts at: 4473 4473
Size: 5297
BspMI ACCTGCnnnn'nnnn_
Cuts at: 284 2347 2561 2863 3244 284
Size: 2063 214 302 381 2337
Fragments arranged by size:
2337 2063 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2204 3589 3643 5127 5292 50
Size: 2154 1385 54 1484 165 55
Fragments arranged by size:
2154 1484 1385 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1463 3209 1463
Size: 1746 3551
BsrFI r'CCGG_y
Cuts at: 223 3295 3476 3685 223
Size: 3072 181 209 1835
Fragments arranged by size:
3072 1835 209 181
BssHII G'CGCG_C
Cuts at: 300 302 300
Size: 2 5295
BssSI C'ACGA_G
Cuts at: 844 1200 3568 3926 844
Size: 356 2368 358 2215
Fragments arranged by size:
2368 2215 358 356
BstAPI GCAn_nnn'nTGC
Cuts at: 2674 2674
Size: 5297
BstBI TT'CG_AA
Cuts at: 2139 3658 2139
Size: 1519 3778
BstYI r'GATC_y
Cuts at: 1 801 1497 2322 2789 2812 3147 3393 4405
Size: 800 696 825 467 23 335 246 1012
Cuts at: 4405 4491 4503 4849 1
Size: 86 12 346 449
Fragments arranged by size:
1012 825 800 696 467 449 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 515 515
Size: 5297
BtgI C'CryG_G
Cuts at: 107 213 1019 1969 107
Size: 106 806 950 3435
Fragments arranged by size:
3435 950 806 106
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1192 3247 3428 3591 1192
Size: 2055 181 163 2898
Fragments arranged by size:
2898 2055 181 163
BtsI GCAGTG_nn'
Cuts at: 2540 2540
Size: 5297
DraI TTT'AAA
Cuts at: 1541 2296 1541
Size: 755 4542
DrdI GACnn_nn'nnGTC
Cuts at: 3003 3861 3003
Size: 858 4439
EaeI y'GGCC_r
Cuts at: 317 2024 2882 3056 3447 3474 4819 317
Size: 1707 858 174 391 27 1345 795
Fragments arranged by size:
1707 1345 858 795 391 174 27
EagI C'GGCC_G
Cuts at: 2882 2882
Size: 5297
EarI CTCTTCn'nnn_
Cuts at: 647 1367 1385 1835 3320 3530 647
Size: 720 18 450 1485 210 2414
Fragments arranged by size:
2414 1485 720 450 210 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 564 1954 3505 3815 3961 5107 564
Size: 1390 1551 310 146 1146 754
Fragments arranged by size:
1551 1390 1146 754 310 146
EcoICRI GAG'CTC
Cuts at: 2309 2309
Size: 5297
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 905 1633 1768 2190 3122 3554 4301 4569
Size: 589 728 135 422 932 432 747 268
Cuts at: 4569 316
Size: 1044
Fragments arranged by size:
1044 932 747 728 589 432 422 268
135
EcoO109I rG'GnC_Cy
Cuts at: 2211 2436 2211
Size: 225 5072
EcoRI G'AATT_C
Cuts at: 2301 2301
Size: 5297
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 840 3316 840
Size: 2476 2821
FspI TGC'GCA
Cuts at: 3078 3078
Size: 5297
HaeII r_GCGC'y
Cuts at: 230 347 2731 2979 4001 4980 230
Size: 117 2384 248 1022 979 547
Fragments arranged by size:
2384 1022 979 547 248 117
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 549 581 1877 1909 2083 2115 3139 3169 3171
Size: 32 1296 32 174 32 1024 30 2
Cuts at: 3171 3201 549
Size: 30 2645
Fragments arranged by size:
2645 1296 1024 174 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1956 2151 2336 1956
Size: 195 185 4917
Fragments arranged by size:
4917 195 185
KasI G'GCGC_C
Cuts at: 226 2727 2975 226
Size: 2501 248 2548
Fragments arranged by size:
2548 2501 248
KpnI G_GTAC'C
Cuts at: 2317 2317
Size: 5297
MluI A'CGCG_T
Cuts at: 4730 4730
Size: 5297
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1062 2102 3968 4152 1062
Size: 1040 1866 184 2207
Fragments arranged by size:
2207 1866 1040 184
MscI TGG'CCA
Cuts at: 2026 3058 2026
Size: 1032 4265
MslI CAynn'nnrTG
Cuts at: 1879 2276 3286 3413 1879
Size: 397 1010 127 3763
Fragments arranged by size:
3763 1010 397 127
MspA1I CmG'CkG
Cuts at: 263 570 695 879 997 1470 1611 2005 2408
Size: 307 125 184 118 473 141 394 403
Cuts at: 2408 2722 3082 4095 4340 263
Size: 314 360 1013 245 1220
Fragments arranged by size:
1220 1013 473 403 394 360 314 307
245 184 141 125 118
NaeI GCC'GGC
Cuts at: 3478 3478
Size: 5297
NarI GG'CG_CC
Cuts at: 227 2728 2976 227
Size: 2501 248 2548
Fragments arranged by size:
2548 2501 248
NcoI C'CATG_G
Cuts at: 107 1019 107
Size: 912 4385
NgoMIV G'CCGG_C
Cuts at: 3476 3476
Size: 5297
NspI r_CATG'y
Cuts at: 983 2350 2850 3757 983
Size: 1367 500 907 2523
Fragments arranged by size:
2523 1367 907 500
PciI A'CATG_T
Cuts at: 3753 3753
Size: 5297
PflMI CCAn_nnn'nTGG
Cuts at: 1020 1047 1020
Size: 27 5270
PmeI GTTT'AAAC
Cuts at: 2296 2296
Size: 5297
PmlI CAC'GTG
Cuts at: 1047 1047
Size: 5297
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4436 4468 4436
Size: 32 5265
PpuMI rG'GwC_Cy
Cuts at: 2211 2211
Size: 5297
PstI C_TGCA'G
Cuts at: 536 914 1710 2120 2344 3029 536
Size: 378 796 410 224 685 2804
Fragments arranged by size:
2804 796 685 410 378 224
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5297
PvuII CAG'CTG
Cuts at: 570 695 879 997 1470 1611 2722 3082 570
Size: 125 184 118 473 141 1111 360 2785
Fragments arranged by size:
2785 1111 473 360 184 141 125 118
RsrII CG'GwC_CG
Cuts at: 3492 3492
Size: 5297
SacI G_AGCT'C
Cuts at: 2311 2311
Size: 5297
SalI G'TCGA_C
Cuts at: 2334 2334
Size: 5297
SapI GCTCTTCn'nnn_
Cuts at: 1367 3320 3530 1367
Size: 1953 210 3134
Fragments arranged by size:
3134 1953 210
SbfI CC_TGCA'GG
Cuts at: 2344 2344
Size: 5297
ScaI AGT'ACT
Cuts at: 4814 4814
Size: 5297
SfcI C'TryA_G
Cuts at: 33 532 910 1706 1793 2116 2340 3025 4018
Size: 499 378 796 87 323 224 685 993
Cuts at: 4018 4209 33
Size: 191 1121
Fragments arranged by size:
1121 993 796 685 499 378 323 224
191 87
SfoI GGC'GCC
Cuts at: 228 2729 2977 228
Size: 2501 248 2548
Fragments arranged by size:
2548 2501 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5297
SmaI CCC'GGG
Cuts at: 384 2319 384
Size: 1935 3362
SmlI C'TyrA_G
Cuts at: 307 555 895 2452 3367 3859 4121 307
Size: 248 340 1557 915 492 262 1483
Fragments arranged by size:
1557 1483 915 492 340 262 248
SpeI A'CTAG_T
Cuts at: 67 795 67
Size: 728 4569
SphI G_CATG'C
Cuts at: 983 2350 2850 983
Size: 1367 500 3430
Fragments arranged by size:
3430 1367 500
StyI C'CwwG_G
Cuts at: 107 809 1019 2431 2785 107
Size: 702 210 1412 354 2619
Fragments arranged by size:
2619 1412 702 354 210
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 185 1258 2880 3013 3472 3681 185
Size: 1073 1622 133 459 209 1801
Fragments arranged by size:
1801 1622 1073 459 209 133
TatI w'GTAC_w
Cuts at: 4812 4812
Size: 5297
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1186 1218 4814 4846 1186
Size: 32 3596 32 1637
Fragments arranged by size:
3596 1637 32 32
Tth111I GACn'n_nGTC
Cuts at: 3094 3094
Size: 5297
XbaI T'CTAG_A
Cuts at: 2328 2328
Size: 5297
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2019 2792 2019
Size: 773 4524
XmaI C'CCGG_G
Cuts at: 382 2317 382
Size: 1935 3362
XmnI GAAnn'nnTTC
Cuts at: 1862 1862
Size: 5297
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PmeI PmlI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BbvCI BcgI BfrBI BglI BmgBI BmtI
BsiWI BsmBI BsrGI BstEII BstXI BstZ17I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PfoI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI