Restriction map for
pF1KA1023
(Circular) (Six-base) MAPSORT of: pF1KA1023.seq Check: 4834 from: 1 to: 5003
pF1KA1023 5003 bp
With 182 enzymes: SgfI *
March 2, 2010 14:24 ..
AatII G_ACGT'C
Cuts at: 1934 1934
Size: 5003
AccI GT'mk_AC
Cuts at: 329 2041 329
Size: 1712 3291
Acc65I G'GTAC_C
Cuts at: 2019 2019
Size: 5003
AclI AA'CG_TT
Cuts at: 4843 4843
Size: 5003
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 249 577 592 865 1132 1543 1814 2828 3260
Size: 328 15 273 267 411 271 1014 432
Cuts at: 3260 4007 4275 249
Size: 747 268 977
Fragments arranged by size:
1014 977 747 432 411 328 273 271
268 267 15
AflIII A'CryG_T
Cuts at: 2355 3459 4436 2355
Size: 1104 977 2922
Fragments arranged by size:
2922 1104 977
AgeI A'CCGG_T
Cuts at: 1783 3391 1783
Size: 1608 3395
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1726 1726
Size: 5003
AlwNI CAG_nnn'CTG
Cuts at: 147 189 477 843 1146 1176 1391 2308 3875
Size: 42 288 366 303 30 215 917 1567
Cuts at: 3875 147
Size: 1275
Fragments arranged by size:
1567 1275 917 366 303 288 215 42
30
ApaLI G'TGCA_C
Cuts at: 3773 3773
Size: 5003
ApoI r'AATT_y
Cuts at: 2007 4356 4612 2007
Size: 2349 256 2398
Fragments arranged by size:
2398 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5003
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5003
AvaI C'yCGr_G
Cuts at: 1763 2023 1763
Size: 260 4743
BamHI G'GATC_C
Cuts at: 480 566 2028 480
Size: 86 1462 3455
Fragments arranged by size:
3455 1462 86
BanI G'GyrC_C
Cuts at: 428 604 760 1911 2019 2433 2681 2716 428
Size: 176 156 1151 108 414 248 35 2715
Fragments arranged by size:
2715 1151 414 248 176 156 108 35
BanII G_rGCy'C
Cuts at: 824 979 1364 1433 1660 1684 2017 3047 824
Size: 155 385 69 227 24 333 1030 2780
Fragments arranged by size:
2780 1030 385 333 227 155 69 24
BbeI G_GCGC'C
Cuts at: 432 764 2437 2685 432
Size: 332 1673 248 2750
Fragments arranged by size:
2750 1673 332 248
BbsI GAAGACnn'nnnn_
Cuts at: 640 760 640
Size: 120 4883
BbvCI CC'TCA_GC
Cuts at: 931 931
Size: 5003
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4969
BciVI GTATCCnnnnn_n'
Cuts at: 2901 3662 5003 2901
Size: 761 1341 2901
Fragments arranged by size:
2901 1341 761
BclI T'GATC_A
Cuts at: 2523 2523
Size: 5003
BglII A'GATC_T
Cuts at: 2518 4555 2518
Size: 2037 2966
BlpI GC'TnA_GC
Cuts at: 868 2115 868
Size: 1247 3756
Bme1580I G_kGCm'C
Cuts at: 792 1670 1755 2628 2721 3777 792
Size: 878 85 873 93 1056 2018
Fragments arranged by size:
2018 1056 878 873 93 85
BmgBI CAC'GTC
Cuts at: 402 402
Size: 5003
BmrI ACTGGGnnnn_n'
Cuts at: 1491 2215 2296 2631 4775 1491
Size: 724 81 335 2144 1719
Fragments arranged by size:
2144 1719 724 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3370 3370
Size: 5003
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 454 604 682 970 1216 1228 454
Size: 150 78 288 246 12 4229
Fragments arranged by size:
4229 288 246 150 78 12
Bpu10I CC'TnA_GC
Cuts at: 796 931 796
Size: 135 4868
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2179 3058 3550 3848 2179
Size: 879 492 298 3334
Fragments arranged by size:
3334 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1100 4707 1100
Size: 3607 1396
BsaAI yAC'GTr
Cuts at: 387 2356 387
Size: 1969 3034
BsaBI GATnn'nnATC
Cuts at: 6 658 2517 4554 6
Size: 652 1859 2037 455
Fragments arranged by size:
2037 1859 652 455
BsaHI Gr'CG_yC
Cuts at: 227 429 761 1472 1847 1931 2434 2682 3384
Size: 202 332 711 375 84 503 248 702
Cuts at: 3384 4880 227
Size: 1496 350
Fragments arranged by size:
1496 711 702 503 375 350 332 248
202 84
BsaWI w'CCGG_w
Cuts at: 727 1783 2192 2713 3391 3665 3812 4223 727
Size: 1056 409 521 678 274 147 411 1507
Fragments arranged by size:
1507 1056 678 521 411 409 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 202 232 1195 1225 1837 1867 1921 1951 202
Size: 30 963 30 612 30 54 30 3254
Fragments arranged by size:
3254 963 612 54 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 451 697 1243 1246 1251 1264 1279 1438 1732
Size: 246 546 3 5 13 15 159 294
Cuts at: 1732 1833 1842 451
Size: 101 9 3612
Fragments arranged by size:
3612 546 294 246 159 101 15 13
9 5 3
BseYI C'CCAG_C
Cuts at: 153 484 1365 1378 1630 2428 3186 3763 4259
Size: 331 881 13 252 798 758 577 496
Cuts at: 4259 153
Size: 897
Fragments arranged by size:
897 881 798 758 577 496 331 252
13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 409 1030 1732 409
Size: 621 702 3680
Fragments arranged by size:
3680 702 621
BsiEI CG_ry'CG
Cuts at: 104 123 2545 2591 3799 104
Size: 19 2422 46 1208 1308
Fragments arranged by size:
2422 1308 1208 46 19
BsiHKAI G_wGCw'C
Cuts at: 824 874 979 1364 1660 2017 2795 2985 3777
Size: 50 105 385 296 357 778 190 792
Cuts at: 3777 824
Size: 2050
Fragments arranged by size:
2050 792 778 385 357 296 190 105
50
BsmBI CGTCTCn'nnnn_
Cuts at: 700 1093 1463 1976 700
Size: 393 370 513 3727
Fragments arranged by size:
3727 513 393 370
Bsp1286I G_dGCh'C
Cuts at: 792 824 874 979 1364 1433 1660 1670 1684
Size: 32 50 105 385 69 227 10 14
Cuts at: 1684 1755 2017 2628 2721 2795 2985 3047 3777
Size: 71 262 611 93 74 190 62 730
Cuts at: 3777 792
Size: 2018
Fragments arranged by size:
2018 730 611 385 262 227 190 105
93 74 71 69 62 50 32 14
10
BspEI T'CCGG_A
Cuts at: 4223 4223
Size: 5003
BspHI T'CATG_A
Cuts at: 4179 4179
Size: 5003
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2053 2267 2569 2950 2053
Size: 214 302 381 4106
Fragments arranged by size:
4106 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1122 1445 1744 3295 3349 4833 4998 50
Size: 1072 323 299 1551 54 1484 165 55
Fragments arranged by size:
1551 1484 1072 323 299 165 55 54
BsrDI GCAATG_nn'
Cuts at: 379 1961 2915 379
Size: 1582 954 2467
Fragments arranged by size:
2467 1582 954
BsrFI r'CCGG_y
Cuts at: 458 1783 3001 3182 3391 458
Size: 1325 1218 181 209 2070
Fragments arranged by size:
2070 1325 1218 209 181
BsrGI T'GTAC_A
Cuts at: 1508 1508
Size: 5003
BssSI C'ACGA_G
Cuts at: 1058 1081 3274 3632 1058
Size: 23 2193 358 2429
Fragments arranged by size:
2429 2193 358 23
BstAPI GCAn_nnn'nTGC
Cuts at: 1146 1176 1391 2380 1146
Size: 30 215 989 3769
Fragments arranged by size:
3769 989 215 30
BstBI TT'CG_AA
Cuts at: 3364 3364
Size: 5003
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 5003
BstYI r'GATC_y
Cuts at: 1 480 498 566 2028 2495 2518 2853 3099
Size: 479 18 68 1462 467 23 335 246
Cuts at: 3099 4111 4197 4209 4555 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1462 1012 479 467 449 346 335 246
86 68 23 18 12
Bsu36I CC'TnA_GG
Cuts at: 1893 1893
Size: 5003
BtgI C'CryG_G
Cuts at: 489 665 1435 1475 1697 489
Size: 176 770 40 222 3795
Fragments arranged by size:
3795 770 222 176 40
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1671 2953 3134 3297 1671
Size: 1282 181 163 3377
Fragments arranged by size:
3377 1282 181 163
BtsI GCAGTG_nn'
Cuts at: 592 792 1389 2246 592
Size: 200 597 857 3349
Fragments arranged by size:
3349 857 597 200
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1889 1924 1889
Size: 35 4968
DraI TTT'AAA
Cuts at: 2002 2002
Size: 5003
DrdI GACnn_nn'nnGTC
Cuts at: 1957 2709 3567 1957
Size: 752 858 3393
Fragments arranged by size:
3393 858 752
EaeI y'GGCC_r
Cuts at: 120 1405 1478 2588 2762 3153 3180 4525 120
Size: 1285 73 1110 174 391 27 1345 598
Fragments arranged by size:
1345 1285 1110 598 391 174 73 27
EagI C'GGCC_G
Cuts at: 120 2588 120
Size: 2468 2535
EarI CTCTTCn'nnn_
Cuts at: 268 388 644 824 1112 1313 1461 1880 3026
Size: 120 256 180 288 201 148 419 1146
Cuts at: 3026 3236 268
Size: 210 2035
Fragments arranged by size:
2035 1146 419 288 256 210 201 180
148 120
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3211 3521 3667 4813 3211
Size: 310 146 1146 3401
Fragments arranged by size:
3401 1146 310 146
EcoICRI GAG'CTC
Cuts at: 822 977 1362 1658 2015 822
Size: 155 385 296 357 3810
Fragments arranged by size:
3810 385 357 296 155
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 249 454 577 592 604 682 865 970 1132
Size: 205 123 15 12 78 183 105 162
Cuts at: 1132 1216 1228 1543 1814 2828 3260 4007 4275
Size: 84 12 315 271 1014 432 747 268
Cuts at: 4275 249
Size: 977
Fragments arranged by size:
1014 977 747 432 315 271 268 205
183 162 123 105 84 78 15 12
12
EcoNI CCTnn'n_nnAGG
Cuts at: 436 847 1198 436
Size: 411 351 4241
Fragments arranged by size:
4241 411 351
EcoO109I rG'GnC_Cy
Cuts at: 186 852 2142 186
Size: 666 1290 3047
Fragments arranged by size:
3047 1290 666
EcoRI G'AATT_C
Cuts at: 2007 2007
Size: 5003
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3022 3022
Size: 5003
FspI TGC'GCA
Cuts at: 2784 2784
Size: 5003
HaeII r_GCGC'y
Cuts at: 294 432 764 2437 2685 3707 4686 294
Size: 138 332 1673 248 1022 979 611
Fragments arranged by size:
1673 1022 979 611 332 248 138
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 267 299 1642 1674 1837 1869 1921 1953 2845
Size: 32 1343 32 163 32 52 32 892
Cuts at: 2845 2875 2877 2907 267
Size: 30 2 30 2363
Fragments arranged by size:
2363 1343 892 163 52 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1935 2042 1935
Size: 107 4896
KasI G'GCGC_C
Cuts at: 428 760 2433 2681 428
Size: 332 1673 248 2750
Fragments arranged by size:
2750 1673 332 248
KpnI G_GTAC'C
Cuts at: 2023 2023
Size: 5003
MluI A'CGCG_T
Cuts at: 4436 4436
Size: 5003
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1973 3674 3858 1973
Size: 1701 184 3118
Fragments arranged by size:
3118 1701 184
MscI TGG'CCA
Cuts at: 2764 2764
Size: 5003
MslI CAynn'nnrTG
Cuts at: 2992 3119 2992
Size: 127 4876
MspA1I CmG'CkG
Cuts at: 449 491 1143 1437 1477 2114 2428 2788 3801
Size: 42 652 294 40 637 314 360 1013
Cuts at: 3801 4046 449
Size: 245 1406
Fragments arranged by size:
1406 1013 652 637 360 314 294 245
42 40
NaeI GCC'GGC
Cuts at: 3184 3184
Size: 5003
NarI GG'CG_CC
Cuts at: 429 761 2434 2682 429
Size: 332 1673 248 2750
Fragments arranged by size:
2750 1673 332 248
NcoI C'CATG_G
Cuts at: 665 665
Size: 5003
NgoMIV G'CCGG_C
Cuts at: 3182 3182
Size: 5003
NspI r_CATG'y
Cuts at: 217 2056 2556 3463 217
Size: 1839 500 907 1757
Fragments arranged by size:
1839 1757 907 500
PasI CC'CwG_GG
Cuts at: 316 741 316
Size: 425 4578
PciI A'CATG_T
Cuts at: 3459 3459
Size: 5003
PfoI T'CCnGG_A
Cuts at: 476 805 815 476
Size: 329 10 4664
Fragments arranged by size:
4664 329 10
PmeI GTTT'AAAC
Cuts at: 2002 2002
Size: 5003
PmlI CAC'GTG
Cuts at: 387 387
Size: 5003
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4142 4174 4142
Size: 32 4971
PpuMI rG'GwC_Cy
Cuts at: 852 852
Size: 5003
PshAI GACnn'nnGTC
Cuts at: 226 325 226
Size: 99 4904
PstI C_TGCA'G
Cuts at: 1136 1151 1181 1493 2050 2735 1136
Size: 15 30 312 557 685 3404
Fragments arranged by size:
3404 685 557 312 30 15
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5003
PvuII CAG'CTG
Cuts at: 1143 2428 2788 1143
Size: 1285 360 3358
Fragments arranged by size:
3358 1285 360
RsrII CG'GwC_CG
Cuts at: 1124 3198 1124
Size: 2074 2929
SacI G_AGCT'C
Cuts at: 824 979 1364 1660 2017 824
Size: 155 385 296 357 3810
Fragments arranged by size:
3810 385 357 296 155
SacII CC_GC'GG
Cuts at: 492 1438 1478 492
Size: 946 40 4017
Fragments arranged by size:
4017 946 40
SalI G'TCGA_C
Cuts at: 2040 2040
Size: 5003
SapI GCTCTTCn'nnn_
Cuts at: 388 1112 1880 3026 3236 388
Size: 724 768 1146 210 2155
Fragments arranged by size:
2155 1146 768 724 210
SbfI CC_TGCA'GG
Cuts at: 1181 1493 2050 1181
Size: 312 557 4134
Fragments arranged by size:
4134 557 312
ScaI AGT'ACT
Cuts at: 4520 4520
Size: 5003
SfcI C'TryA_G
Cuts at: 33 330 1132 1147 1177 1489 2046 2731 3724
Size: 297 802 15 30 312 557 685 993
Cuts at: 3724 3915 33
Size: 191 1121
Fragments arranged by size:
1121 993 802 685 557 312 297 191
30 15
SfoI GGC'GCC
Cuts at: 430 762 2435 2683 430
Size: 332 1673 248 2750
Fragments arranged by size:
2750 1673 332 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5003
SmaI CCC'GGG
Cuts at: 1765 2025 1765
Size: 260 4743
SmlI C'TyrA_G
Cuts at: 2158 3073 3565 3827 2158
Size: 915 492 262 3334
Fragments arranged by size:
3334 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5003
SphI G_CATG'C
Cuts at: 217 2056 2556 217
Size: 1839 500 2664
Fragments arranged by size:
2664 1839 500
SrfI GCCC'GGGC
Cuts at: 1765 1765
Size: 5003
StuI AGG'CCT
Cuts at: 1643 1906 1643
Size: 263 4740
StyI C'CwwG_G
Cuts at: 665 1907 2137 2491 665
Size: 1242 230 354 3177
Fragments arranged by size:
3177 1242 354 230
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 640 1644 2586 2719 3178 3387 640
Size: 1004 942 133 459 209 2256
Fragments arranged by size:
2256 1004 942 459 209 133
TatI w'GTAC_w
Cuts at: 1508 4518 1508
Size: 3010 1993
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 202 234 571 603 1195 1227 4520 4552 202
Size: 32 337 32 592 32 3293 32 653
Fragments arranged by size:
3293 653 592 337 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2800 2800
Size: 5003
XbaI T'CTAG_A
Cuts at: 2034 2034
Size: 5003
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2498 2498
Size: 5003
XmaI C'CCGG_G
Cuts at: 1763 2023 1763
Size: 260 4743
ZraI GAC'GTC
Cuts at: 1932 1932
Size: 5003
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PfoI PmeI PmlI PpiI
PpuMI PshAI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SrfI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BfrBI BglI BmtI BsiWI BsmI BssHII
BstEII BstZ17I ClaI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PflMI PsiI PspOMI PspXI PsrI SanDI SexAI SfiI
SgrAI SnaBI SspI SwaI XhoI XmnI