Restriction map for
pF1KA1031
(Circular) (Six-base) MAPSORT of: pF1KA1031.seq Check: 7073 from: 1 to: 6044
pF1KA1031 6044 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2211 2211
Size: 6044
AccI GT'mk_AC
Cuts at: 163 1207 3082 163
Size: 1044 1875 3125
Fragments arranged by size:
3125 1875 1044
Acc65I G'GTAC_C
Cuts at: 3060 3060
Size: 6044
AclI AA'CG_TT
Cuts at: 5884 5884
Size: 6044
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1756 2905 3869 4301 4654 5316 1756
Size: 1149 964 432 353 662 2484
Fragments arranged by size:
2484 1149 964 662 432 353
AflIII A'CryG_T
Cuts at: 1637 1952 2462 2516 3396 5196 5477 1637
Size: 315 510 54 880 1800 281 2204
Fragments arranged by size:
2204 1800 880 510 315 281 54
AhdI GACnn_n'nnGTC
Cuts at: 921 1824 921
Size: 903 5141
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 973 2932 973
Size: 1959 4085
AlwNI CAG_nnn'CTG
Cuts at: 222 273 594 2744 3349 4787 222
Size: 51 321 2150 605 1438 1479
Fragments arranged by size:
2150 1479 1438 605 321 51
ApaI G_GGCC'C
Cuts at: 1138 2165 1138
Size: 1027 5017
ApaLI G'TGCA_C
Cuts at: 1882 4882 1882
Size: 3000 3044
ApoI r'AATT_y
Cuts at: 3048 5397 5653 3048
Size: 2349 256 3439
Fragments arranged by size:
3439 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6044
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6044
AvaI C'yCGr_G
Cuts at: 338 749 1131 1409 2573 3064 338
Size: 411 382 278 1164 491 3318
Fragments arranged by size:
3318 1164 491 411 382 278
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 973 1006 973
Size: 33 6011
BamHI G'GATC_C
Cuts at: 1511 3069 1511
Size: 1558 4486
BanI G'GyrC_C
Cuts at: 1660 1810 2989 3060 3474 3722 3757 1660
Size: 150 1179 71 414 248 35 3947
Fragments arranged by size:
3947 1179 414 248 150 71 35
BanII G_rGCy'C
Cuts at: 1138 1313 1489 1549 2027 2165 2855 3058 4088
Size: 175 176 60 478 138 690 203 1030
Cuts at: 4088 1138
Size: 3094
Fragments arranged by size:
3094 1030 690 478 203 176 175 138
60
BbeI G_GCGC'C
Cuts at: 3478 3726 3478
Size: 248 5796
BbsI GAAGACnn'nnnn_
Cuts at: 2075 2709 2075
Size: 634 5410
BbvCI CC'TCA_GC
Cuts at: 230 230
Size: 6044
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 295 329 295
Size: 34 6010
BciVI GTATCCnnnnn_n'
Cuts at: 463 3942 4998 6044 463
Size: 3479 1056 1046 463
Fragments arranged by size:
3479 1056 1046 463
BclI T'GATC_A
Cuts at: 3564 3564
Size: 6044
BglI GCCn_nnn'nGGC
Cuts at: 947 947
Size: 6044
BglII A'GATC_T
Cuts at: 1772 3559 5596 1772
Size: 1787 2037 2220
Fragments arranged by size:
2220 2037 1787
BlpI GC'TnA_GC
Cuts at: 3156 3156
Size: 6044
Bme1580I G_kGCm'C
Cuts at: 1138 1663 1886 2165 2929 3669 3762 4886 1138
Size: 525 223 279 764 740 93 1124 2296
Fragments arranged by size:
2296 1124 764 740 525 279 223 93
BmgBI CAC'GTC
Cuts at: 1638 1953 2723 1638
Size: 315 770 4959
Fragments arranged by size:
4959 770 315
BmrI ACTGGGnnnn_n'
Cuts at: 502 604 3041 3256 3337 3672 5816 502
Size: 102 2437 215 81 335 2144 730
Fragments arranged by size:
2437 2144 730 335 215 102 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1272 4411 1272
Size: 3139 2905
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 433 1029 2229 2570 2776 433
Size: 596 1200 341 206 3701
Fragments arranged by size:
3701 1200 596 341 206
Bpu10I CC'TnA_GC
Cuts at: 230 230
Size: 6044
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 1462 1891 2011 2230 3220 4099 4813 5111
Size: 1272 429 120 219 990 879 714 298
Cuts at: 5111 190
Size: 1123
Fragments arranged by size:
1272 1123 990 879 714 429 298 219
120
BsaI GGTCTCn'nnnn_
Cuts at: 1168 1243 5748 1168
Size: 75 4505 1464
Fragments arranged by size:
4505 1464 75
BsaAI yAC'GTr
Cuts at: 741 3397 741
Size: 2656 3388
BsaBI GATnn'nnATC
Cuts at: 6 3558 5595 6
Size: 3552 2037 455
Fragments arranged by size:
3552 2037 455
BsaHI Gr'CG_yC
Cuts at: 498 2735 3475 3723 4425 5921 498
Size: 2237 740 248 702 1496 621
Fragments arranged by size:
2237 1496 740 702 621 248
BsaWI w'CCGG_w
Cuts at: 1481 3233 3754 4432 4843 4990 5264 1481
Size: 1752 521 678 411 147 274 2261
Fragments arranged by size:
2261 1752 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 334 364 334
Size: 30 6014
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1261 1642 1663 2467 2863 1261
Size: 381 21 804 396 4442
Fragments arranged by size:
4442 804 396 381 21
BseYI C'CCAG_C
Cuts at: 432 759 847 974 1692 2164 2744 3469 4227
Size: 327 88 127 718 472 580 725 758
Cuts at: 4227 4892 5300 432
Size: 665 408 1176
Fragments arranged by size:
1176 758 725 718 665 580 472 408
327 127 88
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 235 298 540 1590 235
Size: 63 242 1050 4689
Fragments arranged by size:
4689 1050 242 63
BsiEI CG_ry'CG
Cuts at: 104 1476 2406 3586 3632 4862 104
Size: 1372 930 1180 46 1230 1286
Fragments arranged by size:
1372 1286 1230 1180 930 46
BsiHKAI G_wGCw'C
Cuts at: 1287 1448 1489 1745 1886 1940 2027 2148 2855
Size: 161 41 256 141 54 87 121 707
Cuts at: 2855 3058 3836 4026 4886 1287
Size: 203 778 190 860 2445
Fragments arranged by size:
2445 860 778 707 256 203 190 161
141 121 87 54 41
Bsp1286I G_dGCh'C
Cuts at: 1138 1287 1313 1448 1489 1549 1663 1745 1886
Size: 149 26 135 41 60 114 82 141
Cuts at: 1886 1940 2027 2148 2165 2855 2929 3058 3669
Size: 54 87 121 17 690 74 129 611
Cuts at: 3669 3762 3836 4026 4088 4886 1138
Size: 93 74 190 62 798 2296
Fragments arranged by size:
2296 798 690 611 190 149 141 135
129 121 114 93 87 82 74 74
62 60 54 41 26 17
BspEI T'CCGG_A
Cuts at: 1481 1481
Size: 6044
BspHI T'CATG_A
Cuts at: 2474 2621 4476 2474
Size: 147 1855 4042
Fragments arranged by size:
4042 1855 147
BspMI ACCTGCnnnn'nnnn_
Cuts at: 821 1166 1685 2186 2211 3094 3308 3610 3991
Size: 345 519 501 25 883 214 302 381
Cuts at: 3991 821
Size: 2874
Fragments arranged by size:
2874 883 519 501 381 345 302 214
25
BsrBI CCG'CTC
Cuts at: 50 1653 1895 2289 4336 4390 5874 6039 50
Size: 1603 242 394 2047 54 1484 165 55
Fragments arranged by size:
2047 1603 1484 394 242 165 55 54
BsrDI GCAATG_nn'
Cuts at: 243 3956 243
Size: 3713 2331
BsrFI r'CCGG_y
Cuts at: 527 1475 2870 2986 4042 4223 527
Size: 948 1395 116 1056 181 2348
Fragments arranged by size:
2348 1395 1056 948 181 116
BssSI C'ACGA_G
Cuts at: 1211 1363 4315 5023 1211
Size: 152 2952 708 2232
Fragments arranged by size:
2952 2232 708 152
BstAPI GCAn_nnn'nTGC
Cuts at: 2807 3421 2807
Size: 614 5430
BstBI TT'CG_AA
Cuts at: 4405 4405
Size: 6044
BstXI CCAn_nnnn'nTGG
Cuts at: 1098 1542 1098
Size: 444 5600
BstYI r'GATC_y
Cuts at: 1 1511 1772 1919 2489 2591 3069 3536 3559
Size: 1510 261 147 570 102 478 467 23
Cuts at: 3559 3894 4140 4446 4458 4544 5596 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1510 1052 570 478 467 449 335 306
261 246 147 102 86 23 12
BtgI C'CryG_G
Cuts at: 107 389 2603 2762 2838 2929 107
Size: 282 2214 159 76 91 3222
Fragments arranged by size:
3222 2214 282 159 91 76
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1707 3994 4175 4338 1707
Size: 2287 181 163 3413
Fragments arranged by size:
3413 2287 181 163
BtsI GCAGTG_nn'
Cuts at: 665 1006 1051 1325 2271 2344 2634 2680 3287
Size: 341 45 274 946 73 290 46 607
Cuts at: 3287 665
Size: 3422
Fragments arranged by size:
3422 946 607 341 290 274 73 46
45
DraI TTT'AAA
Cuts at: 3043 3043
Size: 6044
DrdI GACnn_nn'nnGTC
Cuts at: 883 3750 5094 883
Size: 2867 1344 1833
Fragments arranged by size:
2867 1833 1344
EaeI y'GGCC_r
Cuts at: 302 1100 1473 2199 2403 2897 3629 3803 4194
Size: 798 373 726 204 494 732 174 391
Cuts at: 4194 4221 5566 302
Size: 27 1345 780
Fragments arranged by size:
1345 798 780 732 726 494 391 373
204 174 27
EagI C'GGCC_G
Cuts at: 1473 2403 3629 1473
Size: 930 1226 3888
Fragments arranged by size:
3888 1226 930
EarI CTCTTCn'nnn_
Cuts at: 1687 4067 4277 1687
Size: 2380 210 3454
Fragments arranged by size:
3454 2380 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 145 1912 4252 4994 5140 5854 145
Size: 1767 2340 742 146 714 335
Fragments arranged by size:
2340 1767 742 714 335 146
EcoICRI GAG'CTC
Cuts at: 1487 2025 2853 3056 1487
Size: 538 828 203 4475
Fragments arranged by size:
4475 828 538 203
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 433 1029 1756 2229 2570 2776 2905 3869 4301
Size: 596 727 473 341 206 129 964 432
Cuts at: 4301 4654 5316 433
Size: 353 662 1161
Fragments arranged by size:
1161 964 727 662 596 473 432 353
341 206 129
EcoNI CCTnn'n_nnAGG
Cuts at: 1987 2552 1987
Size: 565 5479
EcoO109I rG'GnC_Cy
Cuts at: 626 839 1001 1135 1405 1437 2241 2295 2558
Size: 213 162 134 270 32 804 54 263
Cuts at: 2558 2979 3183 626
Size: 421 204 3487
Fragments arranged by size:
3487 804 421 270 263 213 204 162
134 54 32
EcoRI G'AATT_C
Cuts at: 3048 3048
Size: 6044
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1365 1554 1875 4063 1365
Size: 189 321 2188 3346
Fragments arranged by size:
3346 2188 321 189
FspI TGC'GCA
Cuts at: 3825 3825
Size: 6044
HaeII r_GCGC'y
Cuts at: 412 433 3478 3726 4956 5727 412
Size: 21 3045 248 1230 771 729
Fragments arranged by size:
3045 1230 771 729 248 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 856 888 907 939 1810 1842 3886 3916 3918
Size: 32 19 32 871 32 2044 30 2
Cuts at: 3918 3948 856
Size: 30 2952
Fragments arranged by size:
2952 2044 871 32 32 32 30 30
19 2
HincII GTy'rAC
Cuts at: 164 1208 3083 164
Size: 1044 1875 3125
Fragments arranged by size:
3125 1875 1044
KasI G'GCGC_C
Cuts at: 3474 3722 3474
Size: 248 5796
KpnI G_GTAC'C
Cuts at: 3064 3064
Size: 6044
MluI A'CGCG_T
Cuts at: 5477 5477
Size: 6044
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1862 2750 4803 4987 1862
Size: 888 2053 184 2919
Fragments arranged by size:
2919 2053 888 184
MscI TGG'CCA
Cuts at: 2201 2899 3805 2201
Size: 698 906 4440
Fragments arranged by size:
4440 906 698
MslI CAynn'nnrTG
Cuts at: 2623 4033 4160 2623
Size: 1410 127 4507
Fragments arranged by size:
4507 1410 127
MspA1I CmG'CkG
Cuts at: 953 978 1692 1997 2741 2831 3155 3469 3829
Size: 25 714 305 744 90 324 314 360
Cuts at: 3829 4613 4858 953
Size: 784 245 2139
Fragments arranged by size:
2139 784 744 714 360 324 314 305
245 90 25
NaeI GCC'GGC
Cuts at: 1477 4225 1477
Size: 2748 3296
NarI GG'CG_CC
Cuts at: 3475 3723 3475
Size: 248 5796
NcoI C'CATG_G
Cuts at: 107 389 2762 2838 107
Size: 282 2373 76 3313
Fragments arranged by size:
3313 2373 282 76
NgoMIV G'CCGG_C
Cuts at: 1475 4223 1475
Size: 2748 3296
NspI r_CATG'y
Cuts at: 364 1320 2119 2466 2502 2520 2919 3097 3597
Size: 956 799 347 36 18 399 178 500
Cuts at: 3597 5200 364
Size: 1603 1208
Fragments arranged by size:
1603 1208 956 799 500 399 347 178
36 18
PciI A'CATG_T
Cuts at: 2462 2516 5196 2462
Size: 54 2680 3310
Fragments arranged by size:
3310 2680 54
PflMI CCAn_nnn'nTGG
Cuts at: 594 636 2421 2744 594
Size: 42 1785 323 3894
Fragments arranged by size:
3894 1785 323 42
PfoI T'CCnGG_A
Cuts at: 248 911 1931 2492 248
Size: 663 1020 561 3800
Fragments arranged by size:
3800 1020 663 561
PmeI GTTT'AAAC
Cuts at: 3043 3043
Size: 6044
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4490 4522 4490
Size: 32 6012
PpuMI rG'GwC_Cy
Cuts at: 839 1001 2241 2295 2558 2979 839
Size: 162 1240 54 263 421 3904
Fragments arranged by size:
3904 1240 421 263 162 54
PshAI GACnn'nnGTC
Cuts at: 1268 1268
Size: 6044
PspOMI G'GGCC_C
Cuts at: 1134 2161 1134
Size: 1027 5017
PspXI vC'TCGA_Gb
Cuts at: 749 749
Size: 6044
PstI C_TGCA'G
Cuts at: 152 2045 2183 3091 3776 152
Size: 1893 138 908 685 2420
Fragments arranged by size:
2420 1893 908 685 138
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6044
PvuII CAG'CTG
Cuts at: 978 1997 3469 3829 978
Size: 1019 1472 360 3193
Fragments arranged by size:
3193 1472 1019 360
RsrII CG'GwC_CG
Cuts at: 4239 4239
Size: 6044
SacI G_AGCT'C
Cuts at: 1489 2027 2855 3058 1489
Size: 538 828 203 4475
Fragments arranged by size:
4475 828 538 203
SalI G'TCGA_C
Cuts at: 162 1206 3081 162
Size: 1044 1875 3125
Fragments arranged by size:
3125 1875 1044
SanDI GG'GwC_CC
Cuts at: 1001 2558 1001
Size: 1557 4487
SapI GCTCTTCn'nnn_
Cuts at: 1687 4067 4277 1687
Size: 2380 210 3454
Fragments arranged by size:
3454 2380 210
SbfI CC_TGCA'GG
Cuts at: 3091 3091
Size: 6044
ScaI AGT'ACT
Cuts at: 1912 2050 2095 2251 5561 1912
Size: 138 45 156 3310 2395
Fragments arranged by size:
3310 2395 156 138 45
SexAI A'CCwGG_T
Cuts at: 323 323
Size: 6044
SfcI C'TryA_G
Cuts at: 33 148 2041 2179 2253 3087 3772 4740 4931
Size: 115 1893 138 74 834 685 968 191
Cuts at: 4931 33
Size: 1146
Fragments arranged by size:
1893 1146 968 834 685 191 138 115
74
SfoI GGC'GCC
Cuts at: 3476 3724 3476
Size: 248 5796
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6044
SmaI CCC'GGG
Cuts at: 340 1133 1411 3066 340
Size: 793 278 1655 3318
Fragments arranged by size:
3318 1655 793 278
SmlI C'TyrA_G
Cuts at: 205 749 1441 1906 2026 2245 3199 4114 4828
Size: 544 692 465 120 219 954 915 714
Cuts at: 4828 5090 205
Size: 262 1159
Fragments arranged by size:
1159 954 915 714 692 544 465 262
219 120
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6044
SphI G_CATG'C
Cuts at: 1320 2119 3097 3597 1320
Size: 799 978 500 3767
Fragments arranged by size:
3767 978 799 500
StuI AGG'CCT
Cuts at: 196 511 1378 196
Size: 315 867 4862
Fragments arranged by size:
4862 867 315
StyI C'CwwG_G
Cuts at: 107 389 1349 1814 2261 2282 2312 2762 2838
Size: 282 960 465 447 21 30 450 76
Cuts at: 2838 2900 3178 3532 107
Size: 62 278 354 2619
Fragments arranged by size:
2619 960 465 450 447 354 282 278
76 62 30 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1015 1375 1660 2131 2319 3627 3760 4219 4428
Size: 360 285 471 188 1308 133 459 209
Cuts at: 4428 1015
Size: 2631
Fragments arranged by size:
2631 1308 471 459 360 285 209 188
133
TatI w'GTAC_w
Cuts at: 281 1910 2048 2093 2249 5559 281
Size: 1629 138 45 156 3310 766
Fragments arranged by size:
3310 1629 766 156 138 45
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 334 366 2677 2709 5561 5593 334
Size: 32 2311 32 2852 32 785
Fragments arranged by size:
2852 2311 785 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3841 3841
Size: 6044
XbaI T'CTAG_A
Cuts at: 3075 3075
Size: 6044
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2136 3539 2136
Size: 1403 4641
XhoI C'TCGA_G
Cuts at: 749 749
Size: 6044
XmaI C'CCGG_G
Cuts at: 338 1131 1409 3064 338
Size: 793 278 1655 3318
Fragments arranged by size:
3318 1655 793 278
XmnI GAAnn'nnTTC
Cuts at: 2716 2716
Size: 6044
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AleI AloI AscI AvrII
BfrBI BmtI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PmlI PsiI PsrI SacII SfiI SgrAI
SnaBI SrfI SspI SwaI ZraI