Restriction map for
pF1KA1032
(Circular) (Six-base) MAPSORT of: pF1KA1032.seq Check: 7603 from: 1 to: 8222
pF1KA1032 8222 bp
With 182 enzymes: SgfI *
July 15, 2010 18:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4950 4950
Size: 8222
AccI GT'mk_AC
Cuts at: 5260 5260
Size: 8222
Acc65I G'GTAC_C
Cuts at: 5238 5238
Size: 8222
AclI AA'CG_TT
Cuts at: 8062 8062
Size: 8222
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 530 1142 3380 3958 3968 3985 4240 4252 6047
Size: 612 2238 578 10 17 255 12 1795
Cuts at: 6047 6479 6832 7494 530
Size: 432 353 662 1258
Fragments arranged by size:
2238 1795 1258 662 612 578 432 353
255 17 12 10
AfeI AGC'GCT
Cuts at: 2902 3357 2902
Size: 455 7767
AflIII A'CryG_T
Cuts at: 170 2447 2855 5574 7374 7655 170
Size: 2277 408 2719 1800 281 737
Fragments arranged by size:
2719 2277 1800 737 408 281
AhdI GACnn_n'nnGTC
Cuts at: 1437 2118 3249 1437
Size: 681 1131 6410
Fragments arranged by size:
6410 1131 681
AleI CACnn'nnGTG
Cuts at: 175 328 2513 2854 4532 175
Size: 153 2185 341 1678 3865
Fragments arranged by size:
3865 2185 1678 341 153
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 569 1943 2341 569
Size: 1374 398 6450
Fragments arranged by size:
6450 1374 398
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 241 273 3898 3930 241
Size: 32 3625 32 4533
Fragments arranged by size:
4533 3625 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1899 3336 3564 4115 4732 5527 6965 1899
Size: 1437 228 551 617 795 1438 3156
Fragments arranged by size:
3156 1438 1437 795 617 551 228
ApaI G_GGCC'C
Cuts at: 385 3227 4835 385
Size: 2842 1608 3772
Fragments arranged by size:
3772 2842 1608
ApaLI G'TGCA_C
Cuts at: 1755 7060 1755
Size: 5305 2917
ApoI r'AATT_y
Cuts at: 163 2283 3297 5226 7575 7831 163
Size: 2120 1014 1929 2349 256 554
Fragments arranged by size:
2349 2120 1929 1014 554 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 8222
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8222
AvaI C'yCGr_G
Cuts at: 812 1502 2023 3077 3587 3724 4942 5242 812
Size: 690 521 1054 510 137 1218 300 3792
Fragments arranged by size:
3792 1218 1054 690 521 510 300 137
BamHI G'GATC_C
Cuts at: 344 2191 5247 344
Size: 1847 3056 3319
Fragments arranged by size:
3319 3056 1847
BanI G'GyrC_C
Cuts at: 151 442 1158 1278 1338 2494 2784 3604 5200
Size: 291 716 120 60 1156 290 820 1596
Cuts at: 5200 5238 5652 5900 5935 151
Size: 38 414 248 35 2438
Fragments arranged by size:
2438 1596 1156 820 716 414 291 290
248 120 60 38 35
BanII G_rGCy'C
Cuts at: 385 811 976 1214 1942 2039 3073 3227 4007
Size: 426 165 238 728 97 1034 154 780
Cuts at: 4007 4573 4835 4893 5236 6266 385
Size: 566 262 58 343 1030 2341
Fragments arranged by size:
2341 1034 1030 780 728 566 426 343
262 238 165 154 97 58
BbeI G_GCGC'C
Cuts at: 5656 5904 5656
Size: 248 7974
BbsI GAAGACnn'nnnn_
Cuts at: 1199 1729 2233 3296 3675 4216 4558 4685 1199
Size: 530 504 1063 379 541 342 127 4736
Fragments arranged by size:
4736 1063 541 530 504 379 342 127
BbvCI CC'TCA_GC
Cuts at: 871 871
Size: 8222
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 195 229 2790 2824 195
Size: 34 2561 34 5593
Fragments arranged by size:
5593 2561 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6120 7176 8222 6120
Size: 1056 1046 6120
Fragments arranged by size:
6120 1056 1046
BclI T'GATC_A
Cuts at: 5742 5742
Size: 8222
BglI GCCn_nnn'nGGC
Cuts at: 971 1276 1309 1553 971
Size: 305 33 244 7640
Fragments arranged by size:
7640 305 244 33
BglII A'GATC_T
Cuts at: 434 2027 2048 5737 7774 434
Size: 1593 21 3689 2037 882
Fragments arranged by size:
3689 2037 1593 882 21
BlpI GC'TnA_GC
Cuts at: 913 934 1208 1349 1367 2431 4006 4840 4924
Size: 21 274 141 18 1064 1575 834 84
Cuts at: 4924 5334 913
Size: 410 3801
Fragments arranged by size:
3801 1575 1064 834 410 274 141 84
21 18
Bme1580I G_kGCm'C
Cuts at: 156 337 385 1759 2779 3227 3609 3755 3863
Size: 181 48 1374 1020 448 382 146 108
Cuts at: 3863 4093 4835 5847 5940 7064 156
Size: 230 742 1012 93 1124 1314
Fragments arranged by size:
1374 1314 1124 1020 1012 742 448 382
230 181 146 108 93 48
BmgBI CAC'GTC
Cuts at: 2120 2448 2604 2120
Size: 328 156 7738
Fragments arranged by size:
7738 328 156
BmrI ACTGGGnnnn_n'
Cuts at: 537 1267 4742 5434 5515 5850 7994 537
Size: 730 3475 692 81 335 2144 765
Fragments arranged by size:
3475 2144 765 730 692 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 870 1956 6589 870
Size: 1086 4633 2503
Fragments arranged by size:
4633 2503 1086
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 407 562 1099 1111 1123 1159 1662 2980 3049
Size: 155 537 12 12 36 503 1318 69
Cuts at: 3049 3631 3781 3925 4582 407
Size: 582 150 144 657 4047
Fragments arranged by size:
4047 1318 657 582 537 503 155 150
144 69 36 12 12
Bpu10I CC'TnA_GC
Cuts at: 871 4075 871
Size: 3204 5018
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1972 4341 4417 4531 4762 5152 5398 6277 6991
Size: 2369 76 114 231 390 246 879 714
Cuts at: 6991 7289 1972
Size: 298 2905
Fragments arranged by size:
2905 2369 879 714 390 298 246 231
114 76
BsaI GGTCTCn'nnnn_
Cuts at: 316 1564 7926 316
Size: 1248 6362 612
Fragments arranged by size:
6362 1248 612
BsaAI yAC'GTr
Cuts at: 173 177 5575 173
Size: 4 5398 2820
Fragments arranged by size:
5398 2820 4
BsaBI GATnn'nnATC
Cuts at: 6 2143 5736 7773 6
Size: 2137 3593 2037 455
Fragments arranged by size:
3593 2137 2037 455
BsaHI Gr'CG_yC
Cuts at: 1762 2717 5653 5901 6603 8099 1762
Size: 955 2936 248 702 1496 1885
Fragments arranged by size:
2936 1885 1496 955 702 248
BsaWI w'CCGG_w
Cuts at: 1961 2960 5411 5932 6610 7021 7168 7442 1961
Size: 999 2451 521 678 411 147 274 2741
Fragments arranged by size:
2741 2451 999 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 814 844 988 1018 2293 2323 3047 3077 3799
Size: 30 144 30 1275 30 724 30 722
Cuts at: 3799 3814 3829 3844 3898 3928 5167 5197 814
Size: 15 15 15 54 30 1239 30 3839
Fragments arranged by size:
3839 1275 1239 724 722 144 54 30
30 30 30 30 30 15 15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 391 529 862 1096 1120 1123 1126 1144 1147
Size: 138 333 234 24 3 3 18 3
Cuts at: 1147 1156 1159 1162 1165 1168 1399 1711 2293
Size: 9 3 3 3 3 231 312 582
Cuts at: 2293 2548 2751 3421 3592 3784 5206 391
Size: 255 203 670 171 192 1422 3407
Fragments arranged by size:
3407 1422 670 582 333 312 255 234
231 203 192 171 138 24 18 9
3 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 235 239 616 650 1055 1184 2973 3226 4413
Size: 4 377 34 405 129 1789 253 1187
Cuts at: 4413 4835 4886 5039 5647 6405 7070 7478 235
Size: 422 51 153 608 758 665 408 979
Fragments arranged by size:
1789 1187 979 758 665 608 422 408
405 377 253 153 129 51 34 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 211 704 1842 2626 3583 211
Size: 493 1138 784 957 4850
Fragments arranged by size:
4850 1138 957 784 493
BsiEI CG_ry'CG
Cuts at: 104 2316 2871 4807 5076 5764 5810 7040 104
Size: 2212 555 1936 269 688 46 1230 1286
Fragments arranged by size:
2212 1936 1286 1230 688 555 269 46
BsiHKAI G_wGCw'C
Cuts at: 208 962 1759 1942 1988 2039 2803 3413 3794
Size: 754 797 183 46 51 764 610 381
Cuts at: 3794 4007 5236 6014 6204 7064 208
Size: 213 1229 778 190 860 1366
Fragments arranged by size:
1366 1229 860 797 778 764 754 610
381 213 190 183 51 46
BsiWI C'GTAC_G
Cuts at: 173 173
Size: 8222
BsmI GAATG_Cn'
Cuts at: 3866 3866
Size: 8222
Bsp1286I G_dGCh'C
Cuts at: 156 208 337 385 811 962 976 1214 1759
Size: 52 129 48 426 151 14 238 545
Cuts at: 1759 1942 1988 2039 2779 2803 3073 3227 3413
Size: 183 46 51 740 24 270 154 186
Cuts at: 3413 3609 3755 3794 3863 4007 4093 4573 4835
Size: 196 146 39 69 144 86 480 262
Cuts at: 4835 4893 5236 5847 5940 6014 6204 6266 7064
Size: 58 343 611 93 74 190 62 798
Cuts at: 7064 156
Size: 1314
Fragments arranged by size:
1314 798 740 611 545 480 426 343
270 262 238 196 190 186 183 154
151 146 144 129 93 86 74 69
62 58 52 51 48 46 39 24
14
BspHI T'CATG_A
Cuts at: 2795 3872 6654 2795
Size: 1077 2782 4363
Fragments arranged by size:
4363 2782 1077
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1907 4175 4586 4634 4950 5272 5486 5788 6169
Size: 2268 411 48 316 322 214 302 381
Cuts at: 6169 1907
Size: 3960
Fragments arranged by size:
3960 2268 411 381 322 316 302 214
48
BsrBI CCG'CTC
Cuts at: 50 993 5070 6514 6568 8052 8217 50
Size: 943 4077 1444 54 1484 165 55
Fragments arranged by size:
4077 1484 1444 943 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4104 6134 4104
Size: 2030 6192
BsrFI r'CCGG_y
Cuts at: 6220 6401 6220
Size: 181 8041
BsrGI T'GTAC_A
Cuts at: 3161 4644 3161
Size: 1483 6739
BssHII G'CGCG_C
Cuts at: 4987 4987
Size: 8222
BssSI C'ACGA_G
Cuts at: 1612 1752 1882 6493 7201 1612
Size: 140 130 4611 708 2633
Fragments arranged by size:
4611 2633 708 140 130
BstAPI GCAn_nnn'nTGC
Cuts at: 626 966 1851 1856 1865 2106 4587 5599 626
Size: 340 885 5 9 241 2481 1012 3249
Fragments arranged by size:
3249 2481 1012 885 340 241 9 5
BstBI TT'CG_AA
Cuts at: 1775 3917 6583 1775
Size: 2142 2666 3414
Fragments arranged by size:
3414 2666 2142
BstEII G'GTnAC_C
Cuts at: 2058 2058
Size: 8222
BstXI CCAn_nnnn'nTGG
Cuts at: 540 1635 3090 4413 4534 540
Size: 1095 1455 1323 121 4228
Fragments arranged by size:
4228 1455 1323 1095 121
BstYI r'GATC_y
Cuts at: 1 344 434 1355 2003 2027 2048 2191 2627
Size: 343 90 921 648 24 21 143 436
Cuts at: 2627 5247 5714 5737 6072 6318 6624 6636 6722
Size: 2620 467 23 335 246 306 12 86
Cuts at: 6722 7774 1
Size: 1052 449
Fragments arranged by size:
2620 1052 921 648 467 449 436 343
335 306 246 143 90 86 24 23
21 12
Bsu36I CC'TnA_GG
Cuts at: 1361 1361
Size: 8222
BtgI C'CryG_G
Cuts at: 878 2150 3401 3671 3923 4262 878
Size: 1272 1251 270 252 339 4838
Fragments arranged by size:
4838 1272 1251 339 270 252
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 198 1821 6172 6353 6516 198
Size: 1623 4351 181 163 1904
Fragments arranged by size:
4351 1904 1623 181 163
BtsI GCAGTG_nn'
Cuts at: 692 720 4486 5465 692
Size: 28 3766 979 3449
Fragments arranged by size:
3766 3449 979 28
DraI TTT'AAA
Cuts at: 5221 5221
Size: 8222
DraIII CAC_nnn'GTG
Cuts at: 341 1865 2595 341
Size: 1524 730 5968
Fragments arranged by size:
5968 1524 730
DrdI GACnn_nn'nnGTC
Cuts at: 412 2206 5928 7272 412
Size: 1794 3722 1344 1362
Fragments arranged by size:
3722 1794 1362 1344
EaeI y'GGCC_r
Cuts at: 1301 1625 1787 3188 4804 5012 5073 5147 5807
Size: 324 162 1401 1616 208 61 74 660
Cuts at: 5807 5981 6372 6399 7744 1301
Size: 174 391 27 1345 1779
Fragments arranged by size:
1779 1616 1401 1345 660 391 324 208
174 162 74 61 27
EagI C'GGCC_G
Cuts at: 4804 5073 5807 4804
Size: 269 734 7219
Fragments arranged by size:
7219 734 269
EarI CTCTTCn'nnn_
Cuts at: 506 906 1067 1930 4040 4561 6245 6455 506
Size: 400 161 863 2110 521 1684 210 2273
Fragments arranged by size:
2273 2110 1684 863 521 400 210 161
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 352 1969 2121 5175 6430 7172 7318 8032 352
Size: 1617 152 3054 1255 742 146 714 542
Fragments arranged by size:
3054 1617 1255 742 714 542 152 146
EcoICRI GAG'CTC
Cuts at: 1940 2037 4005 5234 1940
Size: 97 1968 1229 4928
Fragments arranged by size:
4928 1968 1229 97
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 407 530 562 1099 1111 1123 1142 1159 1662
Size: 123 32 537 12 12 19 17 503
Cuts at: 1662 2980 3049 3380 3631 3781 3925 3958 3968
Size: 1318 69 331 251 150 144 33 10
Cuts at: 3968 3985 4240 4252 4582 6047 6479 6832 7494
Size: 17 255 12 330 1465 432 353 662
Cuts at: 7494 407
Size: 1135
Fragments arranged by size:
1465 1318 1135 662 537 503 432 353
331 330 255 251 150 144 123 69
33 32 19 17 17 12 12 12
10
EcoNI CCTnn'n_nnAGG
Cuts at: 1105 4078 1105
Size: 2973 5249
EcoO109I rG'GnC_Cy
Cuts at: 381 382 450 1086 1269 1290 1896 3000 3223
Size: 1 68 636 183 21 606 1104 223
Cuts at: 3223 4832 4939 5361 381
Size: 1609 107 422 3242
Fragments arranged by size:
3242 1609 1104 636 606 422 223 183
107 68 21 1
EcoRI G'AATT_C
Cuts at: 3297 5226 3297
Size: 1929 6293
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3133 4572 6241 3133
Size: 1439 1669 5114
Fragments arranged by size:
5114 1669 1439
FspI TGC'GCA
Cuts at: 6003 6003
Size: 8222
HaeII r_GCGC'y
Cuts at: 2904 3359 3768 4931 5054 5656 5904 7134 7905
Size: 455 409 1163 123 602 248 1230 771
Cuts at: 7905 2904
Size: 3221
Fragments arranged by size:
3221 1230 1163 771 602 455 409 248
123
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 255 287 988 1020 1423 1455 2104 2136 2341
Size: 32 701 32 403 32 649 32 205
Cuts at: 2341 2373 2992 3024 3235 3267 3357 3389 3568
Size: 32 619 32 211 32 90 32 179
Cuts at: 3568 3600 3670 3702 3799 3814 3831 3846 4567
Size: 32 70 32 97 15 17 15 721
Cuts at: 4567 4599 5167 5199 6064 6094 6096 6126 255
Size: 32 568 32 865 30 2 30 2351
Fragments arranged by size:
2351 865 721 701 649 619 568 403
211 205 179 97 90 70 32 32
32 32 32 32 32 32 32 32
32 32 30 30 17 15 15 2
HincII GTy'rAC
Cuts at: 4821 5261 4821
Size: 440 7782
KasI G'GCGC_C
Cuts at: 5652 5900 5652
Size: 248 7974
KpnI G_GTAC'C
Cuts at: 5242 5242
Size: 8222
MluI A'CGCG_T
Cuts at: 7655 7655
Size: 8222
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 105 1001 2336 2891 2909 5153 6981 7165 105
Size: 896 1335 555 18 2244 1828 184 1162
Fragments arranged by size:
2244 1828 1335 1162 896 555 184 18
MscI TGG'CCA
Cuts at: 1303 1627 5149 5983 1303
Size: 324 3522 834 3542
Fragments arranged by size:
3542 3522 834 324
MslI CAynn'nnrTG
Cuts at: 175 217 328 668 1978 1981 2149 2323 2419
Size: 42 111 340 1310 3 168 174 96
Cuts at: 2419 2513 2515 2854 3871 4532 5086 6211 6338
Size: 94 2 339 1017 661 554 1125 127
Cuts at: 6338 175
Size: 2059
Fragments arranged by size:
2059 1310 1125 1017 661 554 340 339
174 168 127 111 96 94 42 3
2
MspA1I CmG'CkG
Cuts at: 239 933 1246 1348 1491 1923 2435 2925 3903
Size: 694 313 102 143 432 512 490 978
Cuts at: 3903 4422 5025 5333 5647 6007 6791 7036 239
Size: 519 603 308 314 360 784 245 1425
Fragments arranged by size:
1425 978 784 694 603 519 512 490
432 360 314 313 308 245 143 102
NaeI GCC'GGC
Cuts at: 6403 6403
Size: 8222
NarI GG'CG_CC
Cuts at: 5653 5901 5653
Size: 248 7974
NcoI C'CATG_G
Cuts at: 3401 3923 4262 3401
Size: 522 339 7361
Fragments arranged by size:
7361 522 339
NgoMIV G'CCGG_C
Cuts at: 6401 6401
Size: 8222
NruI TCG'CGA
Cuts at: 1840 1840
Size: 8222
NspI r_CATG'y
Cuts at: 1581 1854 3655 5275 5775 7378 1581
Size: 273 1801 1620 500 1603 2425
Fragments arranged by size:
2425 1801 1620 1603 500 273
PasI CC'CwG_GG
Cuts at: 804 1252 1549 2163 2779 4662 804
Size: 448 297 614 616 1883 4364
Fragments arranged by size:
4364 1883 616 614 448 297
PciI A'CATG_T
Cuts at: 7374 7374
Size: 8222
PflMI CCAn_nnn'nTGG
Cuts at: 3564 4668 4732 3564
Size: 1104 64 7054
Fragments arranged by size:
7054 1104 64
PfoI T'CCnGG_A
Cuts at: 473 1100 1645 2006 2042 2915 2996 4559 4725
Size: 627 545 361 36 873 81 1563 166
Cuts at: 4725 473
Size: 3970
Fragments arranged by size:
3970 1563 873 627 545 361 166 81
36
PmeI GTTT'AAAC
Cuts at: 5221 5221
Size: 8222
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2569 2601 3898 3930 6668 6700 2569
Size: 32 1297 32 2738 32 4091
Fragments arranged by size:
4091 2738 1297 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 450 1086 1896 3000 4939 450
Size: 636 810 1104 1939 3733
Fragments arranged by size:
3733 1939 1104 810 636
PshAI GACnn'nnGTC
Cuts at: 4937 4937
Size: 8222
PspOMI G'GGCC_C
Cuts at: 381 3223 4831 381
Size: 2842 1608 3772
Fragments arranged by size:
3772 2842 1608
PspXI vC'TCGA_Gb
Cuts at: 3724 3724
Size: 8222
PstI C_TGCA'G
Cuts at: 971 1347 1496 1922 4120 4961 5024 5269 5954
Size: 376 149 426 2198 841 63 245 685
Cuts at: 5954 971
Size: 3239
Fragments arranged by size:
3239 2198 841 685 426 376 245 149
63
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8222
PvuII CAG'CTG
Cuts at: 239 933 1246 1348 1491 2925 3903 4422 5025
Size: 694 313 102 143 1434 978 519 603
Cuts at: 5025 5647 6007 239
Size: 622 360 2454
Fragments arranged by size:
2454 1434 978 694 622 603 519 360
313 143 102
RsrII CG'GwC_CG
Cuts at: 6417 6417
Size: 8222
SacI G_AGCT'C
Cuts at: 1942 2039 4007 5236 1942
Size: 97 1968 1229 4928
Fragments arranged by size:
4928 1968 1229 97
SalI G'TCGA_C
Cuts at: 5259 5259
Size: 8222
SanDI GG'GwC_CC
Cuts at: 4939 4939
Size: 8222
SapI GCTCTTCn'nnn_
Cuts at: 1067 1930 4561 6245 6455 1067
Size: 863 2631 1684 210 2834
Fragments arranged by size:
2834 2631 1684 863 210
SbfI CC_TGCA'GG
Cuts at: 971 5269 971
Size: 4298 3924
ScaI AGT'ACT
Cuts at: 7739 7739
Size: 8222
SfcI C'TryA_G
Cuts at: 33 822 967 1343 1492 1918 4116 4957 5020
Size: 789 145 376 149 426 2198 841 63
Cuts at: 5020 5265 5950 6918 7109 33
Size: 245 685 968 191 1146
Fragments arranged by size:
2198 1146 968 841 789 685 426 376
245 191 149 145 63
SfiI GGCCn_nnn'nGGCC
Cuts at: 1309 1309
Size: 8222
SfoI GGC'GCC
Cuts at: 5654 5902 5654
Size: 248 7974
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8222
SmaI CCC'GGG
Cuts at: 814 1504 3589 5244 814
Size: 690 2085 1655 3792
Fragments arranged by size:
3792 2085 1655 690
SmlI C'TyrA_G
Cuts at: 1987 3724 4320 4432 4546 4777 5167 5377 6292
Size: 1737 596 112 114 231 390 210 915
Cuts at: 6292 7006 7268 1987
Size: 714 262 2941
Fragments arranged by size:
2941 1737 915 714 596 390 262 231
210 114 112
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8222
SphI G_CATG'C
Cuts at: 1581 1854 3655 5275 5775 1581
Size: 273 1801 1620 500 4028
Fragments arranged by size:
4028 1801 1620 500 273
SspI AAT'ATT
Cuts at: 577 784 4522 577
Size: 207 3738 4277
Fragments arranged by size:
4277 3738 207
StyI C'CwwG_G
Cuts at: 446 1304 1376 1448 1670 2639 3401 3818 3842
Size: 858 72 72 222 969 762 417 24
Cuts at: 3842 3923 4262 4406 5150 5356 5710 446
Size: 81 339 144 744 206 354 2958
Fragments arranged by size:
2958 969 858 762 744 417 354 339
222 206 144 81 72 72 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1017 1296 1389 2614 4054 4660 4678 5805 5938
Size: 279 93 1225 1440 606 18 1127 133
Cuts at: 5938 6397 6606 1017
Size: 459 209 2633
Fragments arranged by size:
2633 1440 1225 1127 606 459 279 209
133 93 18
TatI w'GTAC_w
Cuts at: 2561 3134 3161 4644 4898 7737 2561
Size: 573 27 1483 254 2839 3046
Fragments arranged by size:
3046 2839 1483 573 254 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 275 307 2293 2325 7739 7771 275
Size: 32 1986 32 5414 32 726
Fragments arranged by size:
5414 1986 726 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 6019 6019
Size: 8222
XbaI T'CTAG_A
Cuts at: 5253 5253
Size: 8222
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5717 5717
Size: 8222
XhoI C'TCGA_G
Cuts at: 3724 3724
Size: 8222
XmaI C'CCGG_G
Cuts at: 812 1502 3587 5242 812
Size: 690 2085 1655 3792
Fragments arranged by size:
3792 2085 1655 690
XmnI GAAnn'nnTTC
Cuts at: 2584 2968 3913 4555 2584
Size: 384 945 642 6251
Fragments arranged by size:
6251 945 642 384
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NruI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AflII AgeI AscI AvrII BaeI BfrBI BmtI
BsmBI BspEI BstZ17I ClaI CspCI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NsiI PacI
PmlI PsiI PsrI SacII SexAI SgrAI SnaBI SrfI
StuI SwaI ZraI