Restriction map for
pF1KA1040
(Circular) (Six-base) MAPSORT of: pF1KA1040.seq Check: 4686 from: 1 to: 8267
pF1KA1040 8267 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AccI GT'mk_AC
Cuts at: 1687 5305 1687
Size: 3618 4649
Acc65I G'GTAC_C
Cuts at: 5283 5283
Size: 8267
AclI AA'CG_TT
Cuts at: 8107 8107
Size: 8267
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 674 1568 1682 2206 2881 4866 5156 6092 6524
Size: 894 114 524 675 1985 290 936 432
Cuts at: 6524 6877 7539 674
Size: 353 662 1402
Fragments arranged by size:
1985 1402 936 894 675 662 524 432
353 290 114
AflII C'TTAA_G
Cuts at: 472 5077 472
Size: 4605 3662
AflIII A'CryG_T
Cuts at: 5619 7419 7700 5619
Size: 1800 281 6186
Fragments arranged by size:
6186 1800 281
AleI CACnn'nnGTG
Cuts at: 186 186
Size: 8267
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1060 1060
Size: 8267
AlwNI CAG_nnn'CTG
Cuts at: 604 1193 2691 2784 5572 7010 604
Size: 589 1498 93 2788 1438 1861
Fragments arranged by size:
2788 1861 1498 1438 589 93
ApaI G_GGCC'C
Cuts at: 3713 3713
Size: 8267
ApaLI G'TGCA_C
Cuts at: 7105 7105
Size: 8267
ApoI r'AATT_y
Cuts at: 199 272 904 1129 2537 3623 4216 4564 4741
Size: 73 632 225 1408 1086 593 348 177
Cuts at: 4741 4840 5271 7620 7876 199
Size: 99 431 2349 256 590
Fragments arranged by size:
2349 1408 1086 632 593 590 431 348
256 225 177 99 73
AseI AT'TA_AT
Cuts at: 20 2735 20
Size: 2715 5552
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8267
AvaI C'yCGr_G
Cuts at: 127 5287 127
Size: 5160 3107
BamHI G'GATC_C
Cuts at: 3213 5292 3213
Size: 2079 6188
BanI G'GyrC_C
Cuts at: 115 2266 5283 5697 5945 5980 115
Size: 2151 3017 414 248 35 2402
Fragments arranged by size:
3017 2402 2151 414 248 35
BanII G_rGCy'C
Cuts at: 3713 4780 5281 6311 3713
Size: 1067 501 1030 5669
Fragments arranged by size:
5669 1067 1030 501
BbeI G_GCGC'C
Cuts at: 5701 5949 5701
Size: 248 8019
BbsI GAAGACnn'nnnn_
Cuts at: 1780 1893 2887 1780
Size: 113 994 7160
Fragments arranged by size:
7160 994 113
BciVI GTATCCnnnnn_n'
Cuts at: 1454 4190 4509 6165 7221 8267 1454
Size: 2736 319 1656 1056 1046 1454
Fragments arranged by size:
2736 1656 1454 1056 1046 319
BclI T'GATC_A
Cuts at: 2856 5787 2856
Size: 2931 5336
BfrBI ATG'CAT
Cuts at: 754 4054 5156 754
Size: 3300 1102 3865
Fragments arranged by size:
3865 3300 1102
BglI GCCn_nnn'nGGC
Cuts at: 2817 2817
Size: 8267
BglII A'GATC_T
Cuts at: 718 5782 7819 718
Size: 5064 2037 1166
Fragments arranged by size:
5064 2037 1166
BlpI GC'TnA_GC
Cuts at: 5379 5379
Size: 8267
Bme1580I G_kGCm'C
Cuts at: 3713 5121 5892 5985 7109 3713
Size: 1408 771 93 1124 4871
Fragments arranged by size:
4871 1408 1124 771 93
BmgBI CAC'GTC
Cuts at: 3452 3452
Size: 8267
BmrI ACTGGGnnnn_n'
Cuts at: 1294 1900 2294 5479 5560 5895 8039 1294
Size: 606 394 3185 81 335 2144 1522
Fragments arranged by size:
3185 2144 1522 606 394 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 315 6634 315
Size: 6319 1948
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 205 986 3410 3491 4402 4616 4855 169
Size: 36 781 2424 81 911 214 239 3581
Fragments arranged by size:
3581 2424 911 781 239 214 81 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 877 2090 2590 2740 4889 5443 6322 7036 7334
Size: 1213 500 150 2149 554 879 714 298
Cuts at: 7334 877
Size: 1810
Fragments arranged by size:
2149 1810 1213 879 714 554 500 298
150
BsaI GGTCTCn'nnnn_
Cuts at: 2541 7971 2541
Size: 5430 2837
BsaAI yAC'GTr
Cuts at: 5620 5620
Size: 8267
BsaBI GATnn'nnATC
Cuts at: 6 3821 5781 7818 6
Size: 3815 1960 2037 455
Fragments arranged by size:
3815 2037 1960 455
BsaHI Gr'CG_yC
Cuts at: 5698 5946 6648 8144 5698
Size: 248 702 1496 5821
Fragments arranged by size:
5821 1496 702 248
BsaWI w'CCGG_w
Cuts at: 5456 5977 6655 7066 7213 7487 5456
Size: 521 678 411 147 274 6236
Fragments arranged by size:
6236 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1790 1820 3864 3894 5246 5276 1790
Size: 30 2044 30 1352 30 4781
Fragments arranged by size:
4781 2044 1352 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 907 4498 907
Size: 3591 4676
BseYI C'CCAG_C
Cuts at: 5692 6450 7115 7523 5692
Size: 758 665 408 6436
Fragments arranged by size:
6436 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 242 1219 1781 3137 3260 5171 242
Size: 977 562 1356 123 1911 3338
Fragments arranged by size:
3338 1911 1356 977 562 123
BsiEI CG_ry'CG
Cuts at: 104 5809 5855 7085 104
Size: 5705 46 1230 1286
Fragments arranged by size:
5705 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 4418 5281 6059 6249 7109 4418
Size: 863 778 190 860 5576
Fragments arranged by size:
5576 863 860 778 190
BsmI GAATG_Cn'
Cuts at: 1248 1570 1776 2773 2884 3291 5158 1248
Size: 322 206 997 111 407 1867 4357
Fragments arranged by size:
4357 1867 997 407 322 206 111
BsmBI CGTCTCn'nnnn_
Cuts at: 3442 3442
Size: 8267
Bsp1286I G_dGCh'C
Cuts at: 3713 4418 4780 5121 5281 5892 5985 6059 6249
Size: 705 362 341 160 611 93 74 190
Cuts at: 6249 6311 7109 3713
Size: 62 798 4871
Fragments arranged by size:
4871 798 705 611 362 341 190 160
93 74 62
BspHI T'CATG_A
Cuts at: 522 6699 522
Size: 6177 2090
BspMI ACCTGCnnnn'nnnn_
Cuts at: 873 2956 5317 5531 5833 6214 873
Size: 2083 2361 214 302 381 2926
Fragments arranged by size:
2926 2361 2083 381 302 214
BsrBI CCG'CTC
Cuts at: 50 6559 6613 8097 8262 50
Size: 6509 54 1484 165 55
Fragments arranged by size:
6509 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2169 2450 3445 4262 4710 6179 2169
Size: 281 995 817 448 1469 4257
Fragments arranged by size:
4257 1469 995 817 448 281
BsrFI r'CCGG_y
Cuts at: 4302 6265 6446 4302
Size: 1963 181 6123
Fragments arranged by size:
6123 1963 181
BsrGI T'GTAC_A
Cuts at: 309 309
Size: 8267
BssHII G'CGCG_C
Cuts at: 171 171
Size: 8267
BssSI C'ACGA_G
Cuts at: 6538 7246 6538
Size: 708 7559
BstAPI GCAn_nnn'nTGC
Cuts at: 2784 5644 2784
Size: 2860 5407
BstBI TT'CG_AA
Cuts at: 6628 6628
Size: 8267
BstXI CCAn_nnnn'nTGG
Cuts at: 1566 1566
Size: 8267
BstYI r'GATC_y
Cuts at: 1 718 771 1288 3213 3482 3862 4286 4951
Size: 717 53 517 1925 269 380 424 665
Cuts at: 4951 5292 5759 5782 6117 6363 6669 6681 6767
Size: 341 467 23 335 246 306 12 86
Cuts at: 6767 7819 1
Size: 1052 449
Fragments arranged by size:
1925 1052 717 665 517 467 449 424
380 341 335 306 269 246 86 53
23 12
Bsu36I CC'TnA_GG
Cuts at: 4736 4736
Size: 8267
BtgI C'CryG_G
Cuts at: 2175 2175
Size: 8267
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4954 6217 6398 6561 4954
Size: 1263 181 163 6660
Fragments arranged by size:
6660 1263 181 163
BtsI GCAGTG_nn'
Cuts at: 1497 1982 2084 2327 2398 3362 4403 5510 1497
Size: 485 102 243 71 964 1041 1107 4254
Fragments arranged by size:
4254 1107 1041 964 485 243 102 71
DraI TTT'AAA
Cuts at: 1853 3134 4946 5048 5266 1853
Size: 1281 1812 102 218 4854
Fragments arranged by size:
4854 1812 1281 218 102
DrdI GACnn_nn'nnGTC
Cuts at: 5973 7317 5973
Size: 1344 6923
EaeI y'GGCC_r
Cuts at: 2559 2818 3139 4579 5852 6026 6417 6444 7789
Size: 259 321 1440 1273 174 391 27 1345
Cuts at: 7789 2559
Size: 3037
Fragments arranged by size:
3037 1440 1345 1273 391 321 259 174
27
EagI C'GGCC_G
Cuts at: 5852 5852
Size: 8267
EarI CTCTTCn'nnn_
Cuts at: 2225 3083 3110 3229 5015 6290 6500 2225
Size: 858 27 119 1786 1275 210 3992
Fragments arranged by size:
3992 1786 1275 858 210 119 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4321 6475 7217 7363 8077 4321
Size: 2154 742 146 714 4511
Fragments arranged by size:
4511 2154 742 714 146
EcoICRI GAG'CTC
Cuts at: 5279 5279
Size: 8267
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 205 674 986 1568 1682 2206 2881 3410
Size: 36 469 312 582 114 524 675 529
Cuts at: 3410 3491 4402 4616 4855 4866 5156 6092 6524
Size: 81 911 214 239 11 290 936 432
Cuts at: 6524 6877 7539 169
Size: 353 662 897
Fragments arranged by size:
936 911 897 675 662 582 529 524
469 432 353 312 290 239 214 114
81 36 11
EcoNI CCTnn'n_nnAGG
Cuts at: 4907 4907
Size: 8267
EcoO109I rG'GnC_Cy
Cuts at: 2296 3709 5406 2296
Size: 1413 1697 5157
Fragments arranged by size:
5157 1697 1413
EcoRI G'AATT_C
Cuts at: 5271 5271
Size: 8267
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3342 4132 6286 3342
Size: 790 2154 5323
Fragments arranged by size:
5323 2154 790
FspI TGC'GCA
Cuts at: 3272 6048 3272
Size: 2776 5491
FspAI rTGC'GCAy
Cuts at: 3272 3272
Size: 8267
HaeII r_GCGC'y
Cuts at: 5701 5949 7179 7950 5701
Size: 248 1230 771 6018
Fragments arranged by size:
6018 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1693 1725 2028 2060 2380 2412 2547 2579 4164
Size: 32 303 32 320 32 135 32 1585
Cuts at: 4164 4196 4627 4659 6109 6139 6141 6171 1693
Size: 32 431 32 1450 30 2 30 3789
Fragments arranged by size:
3789 1585 1450 431 320 303 135 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1688 1712 2086 5306 1688
Size: 24 374 3220 4649
Fragments arranged by size:
4649 3220 374 24
HindIII A'AGCT_T
Cuts at: 2621 3612 2621
Size: 991 7276
KasI G'GCGC_C
Cuts at: 5697 5945 5697
Size: 248 8019
KpnI G_GTAC'C
Cuts at: 5287 5287
Size: 8267
MfeI C'AATT_G
Cuts at: 251 3263 251
Size: 3012 5255
MluI A'CGCG_T
Cuts at: 7700 7700
Size: 8267
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1499 1561 2024 4314 7026 7210 1499
Size: 62 463 2290 2712 184 2556
Fragments arranged by size:
2712 2556 2290 463 184 62
MscI TGG'CCA
Cuts at: 2561 2820 4581 6028 2561
Size: 259 1761 1447 4800
Fragments arranged by size:
4800 1761 1447 259
MslI CAynn'nnrTG
Cuts at: 186 1564 1609 2174 2889 6256 6383 186
Size: 1378 45 565 715 3367 127 2070
Fragments arranged by size:
3367 2070 1378 715 565 127 45
MspA1I CmG'CkG
Cuts at: 418 601 4248 5378 5692 6052 6836 7081 418
Size: 183 3647 1130 314 360 784 245 1604
Fragments arranged by size:
3647 1604 1130 784 360 314 245 183
NaeI GCC'GGC
Cuts at: 6448 6448
Size: 8267
NarI GG'CG_CC
Cuts at: 5698 5946 5698
Size: 248 8019
NcoI C'CATG_G
Cuts at: 2175 2175
Size: 8267
NgoMIV G'CCGG_C
Cuts at: 6446 6446
Size: 8267
NsiI A_TGCA'T
Cuts at: 756 4056 5158 756
Size: 3300 1102 3865
Fragments arranged by size:
3865 3300 1102
NspI r_CATG'y
Cuts at: 438 1041 4170 4552 5320 5820 7423 438
Size: 603 3129 382 768 500 1603 1282
Fragments arranged by size:
3129 1603 1282 768 603 500 382
PciI A'CATG_T
Cuts at: 7419 7419
Size: 8267
PflMI CCAn_nnn'nTGG
Cuts at: 4203 4240 4203
Size: 37 8230
PfoI T'CCnGG_A
Cuts at: 767 3617 767
Size: 2850 5417
PmeI GTTT'AAAC
Cuts at: 5266 5266
Size: 8267
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6713 6745 6713
Size: 32 8235
PpuMI rG'GwC_Cy
Cuts at: 2296 2296
Size: 8267
PshAI GACnn'nnGTC
Cuts at: 387 387
Size: 8267
PsiI TTA'TAA
Cuts at: 1921 1921
Size: 8267
PspOMI G'GGCC_C
Cuts at: 3709 3709
Size: 8267
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 461 493 674 706 1046 1078 461
Size: 32 181 32 340 32 7650
Fragments arranged by size:
7650 340 181 32 32 32
PstI C_TGCA'G
Cuts at: 417 1989 2696 2789 3369 3512 5314 5999 417
Size: 1572 707 93 580 143 1802 685 2685
Fragments arranged by size:
2685 1802 1572 707 685 580 143 93
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8267
PvuII CAG'CTG
Cuts at: 418 601 4248 5692 6052 418
Size: 183 3647 1444 360 2633
Fragments arranged by size:
3647 2633 1444 360 183
RsrII CG'GwC_CG
Cuts at: 6462 6462
Size: 8267
SacI G_AGCT'C
Cuts at: 5281 5281
Size: 8267
SalI G'TCGA_C
Cuts at: 1686 5304 1686
Size: 3618 4649
SapI GCTCTTCn'nnn_
Cuts at: 6290 6500 6290
Size: 210 8057
SbfI CC_TGCA'GG
Cuts at: 5314 5314
Size: 8267
ScaI AGT'ACT
Cuts at: 692 3873 5061 7784 692
Size: 3181 1188 2723 1175
Fragments arranged by size:
3181 2723 1188 1175
SexAI A'CCwGG_T
Cuts at: 3847 3847
Size: 8267
SfcI C'TryA_G
Cuts at: 33 413 608 1985 2288 2692 2785 3365 3508
Size: 380 195 1377 303 404 93 580 143
Cuts at: 3508 3677 5310 5995 6963 7154 33
Size: 169 1633 685 968 191 1146
Fragments arranged by size:
1633 1377 1146 968 685 580 404 380
303 195 191 169 143 93
SfiI GGCCn_nnn'nGGCC
Cuts at: 2817 2817
Size: 8267
SfoI GGC'GCC
Cuts at: 5699 5947 5699
Size: 248 8019
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8267
SgrAI Cr'CCGG_yG
Cuts at: 4302 4302
Size: 8267
SmaI CCC'GGG
Cuts at: 5289 5289
Size: 8267
SmlI C'TyrA_G
Cuts at: 472 856 2105 2569 2755 4904 5077 5422 6337
Size: 384 1249 464 186 2149 173 345 915
Cuts at: 6337 7051 7313 472
Size: 714 262 1426
Fragments arranged by size:
2149 1426 1249 915 714 464 384 345
262 186 173
SpeI A'CTAG_T
Cuts at: 67 5202 67
Size: 5135 3132
SphI G_CATG'C
Cuts at: 1041 4552 5320 5820 1041
Size: 3511 768 500 3488
Fragments arranged by size:
3511 3488 768 500
SspI AAT'ATT
Cuts at: 585 1848 2733 3000 3033 3402 4425 4674 585
Size: 1263 885 267 33 369 1023 249 4178
Fragments arranged by size:
4178 1263 1023 885 369 267 249 33
StyI C'CwwG_G
Cuts at: 338 1256 2175 5014 5401 5755 338
Size: 918 919 2839 387 354 2850
Fragments arranged by size:
2850 2839 919 918 387 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3187 3807 5850 5983 6442 6651 3187
Size: 620 2043 133 459 209 4803
Fragments arranged by size:
4803 2043 620 459 209 133
TatI w'GTAC_w
Cuts at: 309 690 2302 2935 3871 4113 4962 5059 7782
Size: 381 1612 633 936 242 849 97 2723
Cuts at: 7782 309
Size: 794
Fragments arranged by size:
2723 1612 936 849 794 633 381 242
97
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1790 1822 7784 7816 1790
Size: 32 5962 32 2241
Fragments arranged by size:
5962 2241 32 32
Tth111I GACn'n_nGTC
Cuts at: 6064 6064
Size: 8267
XbaI T'CTAG_A
Cuts at: 2436 5298 2436
Size: 2862 5405
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5762 5762
Size: 8267
XmaI C'CCGG_G
Cuts at: 5287 5287
Size: 8267
XmnI GAAnn'nnTTC
Cuts at: 199 3613 3747 4607 199
Size: 3414 134 860 3859
Fragments arranged by size:
3859 3414 860 134
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AloI AscI AvrII
BaeI BbvCI BcgI BmtI Bpu10I BsiWI BspEI BstEII
BstZ17I ClaI CspCI DraIII EcoRV FseI HpaI NdeI
NheI NotI NruI PacI PasI PmlI PspXI SacII
SanDI SnaBI SrfI StuI SwaI XhoI ZraI