Restriction map for
pF1KA1042
(Circular) (Six-base) MAPSORT of: pF1KA1042.seq Check: 6259 from: 1 to: 5972
pF1KA1042 5972 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 555 974 555
Size: 419 5553
AccI GT'mk_AC
Cuts at: 3010 3010
Size: 5972
Acc65I G'GTAC_C
Cuts at: 2775 2988 2775
Size: 213 5759
AclI AA'CG_TT
Cuts at: 5812 5812
Size: 5972
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 616 829 1168 1782 2129 3797 4229 4976 5244
Size: 213 339 614 347 1668 432 747 268
Cuts at: 5244 616
Size: 1344
Fragments arranged by size:
1668 1344 747 614 432 347 339 268
213
AfeI AGC'GCT
Cuts at: 648 648
Size: 5972
AflIII A'CryG_T
Cuts at: 2612 3324 4428 5405 2612
Size: 712 1104 977 3179
Fragments arranged by size:
3179 1104 977 712
AgeI A'CCGG_T
Cuts at: 4360 4360
Size: 5972
AleI CACnn'nnGTG
Cuts at: 2230 2875 2230
Size: 645 5327
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1023 1138 1279 1023
Size: 115 141 5716
Fragments arranged by size:
5716 141 115
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1633 1665 1633
Size: 32 5940
AlwNI CAG_nnn'CTG
Cuts at: 153 849 1041 1686 3277 4844 153
Size: 696 192 645 1591 1567 1281
Fragments arranged by size:
1591 1567 1281 696 645 192
ApaI G_GGCC'C
Cuts at: 2738 2738
Size: 5972
ApaLI G'TGCA_C
Cuts at: 2508 4742 2508
Size: 2234 3738
ApoI r'AATT_y
Cuts at: 2976 5325 5581 2976
Size: 2349 256 3367
Fragments arranged by size:
3367 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5972
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5972
AvaI C'yCGr_G
Cuts at: 667 2159 2739 2919 2992 667
Size: 1492 580 180 73 3647
Fragments arranged by size:
3647 1492 580 180 73
BamHI G'GATC_C
Cuts at: 2997 2997
Size: 5972
BanI G'GyrC_C
Cuts at: 1570 2775 2988 3402 3650 3685 1570
Size: 1205 213 414 248 35 3857
Fragments arranged by size:
3857 1205 414 248 213 35
BanII G_rGCy'C
Cuts at: 147 668 2156 2327 2404 2518 2691 2738 2986
Size: 521 1488 171 77 114 173 47 248
Cuts at: 2986 4016 147
Size: 1030 2103
Fragments arranged by size:
2103 1488 1030 521 248 173 171 114
77 47
BbeI G_GCGC'C
Cuts at: 3406 3654 3406
Size: 248 5724
BbsI GAAGACnn'nnnn_
Cuts at: 817 931 817
Size: 114 5858
BbvCI CC'TCA_GC
Cuts at: 874 1603 2067 874
Size: 729 464 4779
Fragments arranged by size:
4779 729 464
BciVI GTATCCnnnnn_n'
Cuts at: 3870 4631 5972 3870
Size: 761 1341 3870
Fragments arranged by size:
3870 1341 761
BclI T'GATC_A
Cuts at: 3492 3492
Size: 5972
BglI GCCn_nnn'nGGC
Cuts at: 2742 2742
Size: 5972
BglII A'GATC_T
Cuts at: 1354 3487 5524 1354
Size: 2133 2037 1802
Fragments arranged by size:
2133 2037 1802
BlpI GC'TnA_GC
Cuts at: 146 2939 3084 146
Size: 2793 145 3034
Fragments arranged by size:
3034 2793 145
Bme1580I G_kGCm'C
Cuts at: 1573 1753 2512 2738 3597 3690 4746 1573
Size: 180 759 226 859 93 1056 2799
Fragments arranged by size:
2799 1056 859 759 226 180 93
BmgBI CAC'GTC
Cuts at: 1184 2613 1184
Size: 1429 4543
BmrI ACTGGGnnnn_n'
Cuts at: 3184 3265 3600 5744 3184
Size: 81 335 2144 3412
Fragments arranged by size:
3412 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 726 4339 726
Size: 3613 2359
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 320 577 1114 1271 1612 1663 1813 2030
Size: 52 257 537 157 341 51 150 217
Cuts at: 2030 2177 268
Size: 147 4063
Fragments arranged by size:
4063 537 341 257 217 157 150 147
52 51
Bpu10I CC'TnA_GC
Cuts at: 874 1052 1603 2067 874
Size: 178 551 464 4779
Fragments arranged by size:
4779 551 464 178
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 484 3148 4027 4519 4817 484
Size: 2664 879 492 298 1639
Fragments arranged by size:
2664 1639 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 811 2056 5676 811
Size: 1245 3620 1107
Fragments arranged by size:
3620 1245 1107
BsaAI yAC'GTr
Cuts at: 3325 3325
Size: 5972
BsaBI GATnn'nnATC
Cuts at: 6 3486 5523 6
Size: 3480 2037 455
Fragments arranged by size:
3480 2037 455
BsaHI Gr'CG_yC
Cuts at: 1564 3403 3651 4353 5849 1564
Size: 1839 248 702 1496 1687
Fragments arranged by size:
1839 1687 1496 702 248
BsaWI w'CCGG_w
Cuts at: 222 1157 1943 3161 3682 4360 4634 4781 5192
Size: 935 786 1218 521 678 274 147 411
Cuts at: 5192 222
Size: 1002
Fragments arranged by size:
1218 1002 935 786 678 521 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 574 604 1087 1117 1252 1282 1353 1383 1633
Size: 30 483 30 135 30 71 30 250
Cuts at: 1633 1663 2028 2058 2502 2532 574
Size: 30 365 30 444 30 4014
Fragments arranged by size:
4014 483 444 365 250 135 71 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 265 481 574 598 670 702 787 847 967
Size: 216 93 24 72 32 85 60 120
Cuts at: 967 1156 1659 1672 1675 2716 2725 265
Size: 189 503 13 3 1041 9 3512
Fragments arranged by size:
3512 1041 503 216 189 120 93 85
72 60 32 24 13 9 3
BseYI C'CCAG_C
Cuts at: 1061 1203 2381 3397 4155 4732 5228 1061
Size: 142 1178 1016 758 577 496 1805
Fragments arranged by size:
1805 1178 1016 758 577 496 142
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2395 2395
Size: 5972
BsiEI CG_ry'CG
Cuts at: 104 2397 3514 3560 4768 104
Size: 2293 1117 46 1208 1308
Fragments arranged by size:
2293 1308 1208 1117 46
BsiHKAI G_wGCw'C
Cuts at: 2156 2314 2512 2986 3764 3954 4746 2156
Size: 158 198 474 778 190 792 3382
Fragments arranged by size:
3382 792 778 474 198 190 158
BsmBI CGTCTCn'nnnn_
Cuts at: 370 1190 1588 2625 370
Size: 820 398 1037 3717
Fragments arranged by size:
3717 1037 820 398
Bsp1286I G_dGCh'C
Cuts at: 147 668 1573 1753 2156 2314 2327 2404 2512
Size: 521 905 180 403 158 13 77 108
Cuts at: 2512 2518 2691 2738 2986 3597 3690 3764 3954
Size: 6 173 47 248 611 93 74 190
Cuts at: 3954 4016 4746 147
Size: 62 730 1373
Fragments arranged by size:
1373 905 730 611 521 403 248 190
180 173 158 108 93 77 74 62
47 13 6
BspEI T'CCGG_A
Cuts at: 222 1157 1943 5192 222
Size: 935 786 3249 1002
Fragments arranged by size:
3249 1002 935 786
BspHI T'CATG_A
Cuts at: 5148 5148
Size: 5972
BspMI ACCTGCnnnn'nnnn_
Cuts at: 555 974 1823 3022 3236 3538 3919 555
Size: 419 849 1199 214 302 381 2608
Fragments arranged by size:
2608 1199 849 419 381 302 214
BsrBI CCG'CTC
Cuts at: 50 477 496 1545 1808 2346 4264 4318 5802
Size: 427 19 1049 263 538 1918 54 1484
Cuts at: 5802 5967 50
Size: 165 55
Fragments arranged by size:
1918 1484 1049 538 427 263 165 55
54 19
BsrDI GCAATG_nn'
Cuts at: 3884 3884
Size: 5972
BsrFI r'CCGG_y
Cuts at: 1777 2229 2392 3970 4151 4360 1777
Size: 452 163 1578 181 209 3389
Fragments arranged by size:
3389 1578 452 209 181 163
BssSI C'ACGA_G
Cuts at: 2326 2511 2635 4243 4601 2326
Size: 185 124 1608 358 3697
Fragments arranged by size:
3697 1608 358 185 124
BstAPI GCAn_nnn'nTGC
Cuts at: 3349 3349
Size: 5972
BstBI TT'CG_AA
Cuts at: 4333 4333
Size: 5972
BstEII G'GTnAC_C
Cuts at: 1929 2008 1929
Size: 79 5893
BstXI CCAn_nnnn'nTGG
Cuts at: 114 2232 2772 2877 114
Size: 2118 540 105 3209
Fragments arranged by size:
3209 2118 540 105
BstYI r'GATC_y
Cuts at: 1 1354 2881 2997 3464 3487 3822 4068 5080
Size: 1353 1527 116 467 23 335 246 1012
Cuts at: 5080 5166 5178 5524 1
Size: 86 12 346 449
Fragments arranged by size:
1527 1353 1012 467 449 346 335 246
116 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2563 2780 2563
Size: 217 5755
BtgI C'CryG_G
Cuts at: 107 168 1217 2251 2873 107
Size: 61 1049 1034 622 3206
Fragments arranged by size:
3206 1049 1034 622 61
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3922 4103 4266 3922
Size: 181 163 5628
Fragments arranged by size:
5628 181 163
BtsI GCAGTG_nn'
Cuts at: 1019 3215 1019
Size: 2196 3776
DraI TTT'AAA
Cuts at: 2971 2971
Size: 5972
DrdI GACnn_nn'nnGTC
Cuts at: 2613 2617 3678 4536 2613
Size: 4 1061 858 4049
Fragments arranged by size:
4049 1061 858 4
EaeI y'GGCC_r
Cuts at: 414 491 504 917 1403 2234 2394 2663 3557
Size: 77 13 413 486 831 160 269 894
Cuts at: 3557 3731 4122 4149 5494 414
Size: 174 391 27 1345 892
Fragments arranged by size:
1345 894 892 831 486 413 391 269
174 160 77 27 13
EagI C'GGCC_G
Cuts at: 2394 3557 2394
Size: 1163 4809
EarI CTCTTCn'nnn_
Cuts at: 368 688 749 764 1268 3995 4205 368
Size: 320 61 15 504 2727 210 2135
Fragments arranged by size:
2727 2135 504 320 210 61 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1705 2441 2868 4180 4490 4636 5782 1705
Size: 736 427 1312 310 146 1146 1895
Fragments arranged by size:
1895 1312 1146 736 427 310 146
EcoICRI GAG'CTC
Cuts at: 2154 2984 2154
Size: 830 5142
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 320 577 616 829 1114 1168 1271 1612
Size: 52 257 39 213 285 54 103 341
Cuts at: 1612 1663 1782 1813 2030 2129 2177 3797 4229
Size: 51 119 31 217 99 48 1620 432
Cuts at: 4229 4976 5244 268
Size: 747 268 996
Fragments arranged by size:
1620 996 747 432 341 285 268 257
217 213 119 103 99 54 52 51
48 39 31
EcoNI CCTnn'n_nnAGG
Cuts at: 155 247 769 155
Size: 92 522 5358
Fragments arranged by size:
5358 522 92
EcoO109I rG'GnC_Cy
Cuts at: 2719 2735 2784 3111 2719
Size: 16 49 327 5580
Fragments arranged by size:
5580 327 49 16
EcoRI G'AATT_C
Cuts at: 2976 2976
Size: 5972
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1329 3991 1329
Size: 2662 3310
FseI GG_CCGG'CC
Cuts at: 2396 2396
Size: 5972
FspI TGC'GCA
Cuts at: 1011 1255 1708 3753 1011
Size: 244 453 2045 3230
Fragments arranged by size:
3230 2045 453 244
HaeII r_GCGC'y
Cuts at: 650 1194 1603 3406 3654 4676 5655 650
Size: 544 409 1803 248 1022 979 967
Fragments arranged by size:
1803 1022 979 967 544 409 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 574 606 655 687 1267 1299 1353 1385 2678
Size: 32 49 32 580 32 54 32 1293
Cuts at: 2678 2710 2711 2743 2886 2918 3814 3844 3846
Size: 32 1 32 143 32 896 30 2
Cuts at: 3846 3876 574
Size: 30 2670
Fragments arranged by size:
2670 1293 896 580 143 54 49 32
32 32 32 32 32 32 30 30
2 1
HincII GTy'rAC
Cuts at: 1959 3011 1959
Size: 1052 4920
HindIII A'AGCT_T
Cuts at: 1004 1004
Size: 5972
KasI G'GCGC_C
Cuts at: 3402 3650 3402
Size: 248 5724
KpnI G_GTAC'C
Cuts at: 2779 2992 2779
Size: 213 5759
MluI A'CGCG_T
Cuts at: 5405 5405
Size: 5972
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1250 1415 2648 4643 4827 1250
Size: 165 1233 1995 184 2395
Fragments arranged by size:
2395 1995 1233 184 165
MscI TGG'CCA
Cuts at: 416 919 2236 2665 3733 416
Size: 503 1317 429 1068 2655
Fragments arranged by size:
2655 1317 1068 503 429
MslI CAynn'nnrTG
Cuts at: 1120 2230 2875 2893 2929 3961 4088 1120
Size: 1110 645 18 36 1032 127 3004
Fragments arranged by size:
3004 1110 1032 645 127 36 18
MspA1I CmG'CkG
Cuts at: 258 807 849 1065 1427 1712 1845 3083 3397
Size: 549 42 216 362 285 133 1238 314
Cuts at: 3397 3757 4770 5015 258
Size: 360 1013 245 1215
Fragments arranged by size:
1238 1215 1013 549 362 360 314 285
245 216 133 42
NaeI GCC'GGC
Cuts at: 2394 4153 2394
Size: 1759 4213
NarI GG'CG_CC
Cuts at: 3403 3651 3403
Size: 248 5724
NcoI C'CATG_G
Cuts at: 107 1217 2873 107
Size: 1110 1656 3206
Fragments arranged by size:
3206 1656 1110
NgoMIV G'CCGG_C
Cuts at: 2392 4151 2392
Size: 1759 4213
NspI r_CATG'y
Cuts at: 1794 2094 2892 3025 3525 4432 1794
Size: 300 798 133 500 907 3334
Fragments arranged by size:
3334 907 798 500 300 133
PasI CC'CwG_GG
Cuts at: 1746 2319 1746
Size: 573 5399
PciI A'CATG_T
Cuts at: 4428 4428
Size: 5972
PflMI CCAn_nnn'nTGG
Cuts at: 1041 1203 1590 1686 2200 1041
Size: 162 387 96 514 4813
Fragments arranged by size:
4813 514 387 162 96
PfoI T'CCnGG_A
Cuts at: 1679 1907 1679
Size: 228 5744
PmeI GTTT'AAAC
Cuts at: 2971 2971
Size: 5972
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1621 1633 1653 1665 5111 5143 1621
Size: 12 20 12 3446 32 2450
Fragments arranged by size:
3446 2450 32 20 12 12
PpuMI rG'GwC_Cy
Cuts at: 2719 2719
Size: 5972
PsiI TTA'TAA
Cuts at: 269 269
Size: 5972
PspOMI G'GGCC_C
Cuts at: 2734 2734
Size: 5972
PstI C_TGCA'G
Cuts at: 1268 2834 3019 3704 1268
Size: 1566 185 685 3536
Fragments arranged by size:
3536 1566 685 185
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5972
PvuII CAG'CTG
Cuts at: 258 807 849 1427 3397 3757 258
Size: 549 42 578 1970 360 2473
Fragments arranged by size:
2473 1970 578 549 360 42
RsrII CG'GwC_CG
Cuts at: 4167 4167
Size: 5972
SacI G_AGCT'C
Cuts at: 2156 2986 2156
Size: 830 5142
SalI G'TCGA_C
Cuts at: 3009 3009
Size: 5972
SanDI GG'GwC_CC
Cuts at: 2719 2719
Size: 5972
SapI GCTCTTCn'nnn_
Cuts at: 3995 4205 3995
Size: 210 5762
SbfI CC_TGCA'GG
Cuts at: 3019 3019
Size: 5972
ScaI AGT'ACT
Cuts at: 5489 5489
Size: 5972
SexAI A'CCwGG_T
Cuts at: 1337 1337
Size: 5972
SfcI C'TryA_G
Cuts at: 33 1264 2830 3015 3700 4693 4884 33
Size: 1231 1566 185 685 993 191 1121
Fragments arranged by size:
1566 1231 1121 993 685 191 185
SfiI GGCCn_nnn'nGGCC
Cuts at: 2742 2742
Size: 5972
SfoI GGC'GCC
Cuts at: 3404 3652 3404
Size: 248 5724
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5972
SgrAI Cr'CCGG_yG
Cuts at: 2229 2229
Size: 5972
SmaI CCC'GGG
Cuts at: 2741 2994 2741
Size: 253 5719
SmlI C'TyrA_G
Cuts at: 463 3127 4042 4534 4796 463
Size: 2664 915 492 262 1639
Fragments arranged by size:
2664 1639 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5972
SphI G_CATG'C
Cuts at: 2892 3025 3525 2892
Size: 133 500 5339
Fragments arranged by size:
5339 500 133
StuI AGG'CCT
Cuts at: 2771 2771
Size: 5972
StyI C'CwwG_G
Cuts at: 107 1217 1226 1896 1934 2873 3106 3460 107
Size: 1110 9 670 38 939 233 354 2619
Fragments arranged by size:
2619 1110 939 670 354 233 38 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1740 2072 2490 2623 2923 3555 3688 4147 4356
Size: 332 418 133 300 632 133 459 209
Cuts at: 4356 1740
Size: 3356
Fragments arranged by size:
3356 632 459 418 332 300 209 133
133
TatI w'GTAC_w
Cuts at: 783 1973 5487 783
Size: 1190 3514 1268
Fragments arranged by size:
3514 1268 1190
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 204 236 2206 2238 2502 2534 5489 5521 204
Size: 32 1970 32 264 32 2955 32 655
Fragments arranged by size:
2955 1970 655 264 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2644 3769 2644
Size: 1125 4847
XbaI T'CTAG_A
Cuts at: 3003 3003
Size: 5972
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1224 1894 3467 1224
Size: 670 1573 3729
Fragments arranged by size:
3729 1573 670
XmaI C'CCGG_G
Cuts at: 2739 2992 2739
Size: 253 5719
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PsiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AscI AvrII BaeI BcgI BfrBI
BmtI BsiWI BsmI BsrGI BssHII BstZ17I ClaI CspCI
DraIII EcoRV FspAI HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PmlI PshAI PspXI PsrI SacII
SnaBI SrfI SspI SwaI XhoI XmnI ZraI