Restriction map for
pF1KA1047
(Circular) (Six-base) MAPSORT of: pF1KA1047.seq Check: 6787 from: 1 to: 10124
pF1KA1047 10124 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4853 5751 4853
Size: 898 9226
AccI GT'mk_AC
Cuts at: 187 4690 7162 187
Size: 4503 2472 3149
Fragments arranged by size:
4503 3149 2472
Acc65I G'GTAC_C
Cuts at: 3556 7140 3556
Size: 3584 6540
AclI AA'CG_TT
Cuts at: 9964 9964
Size: 10124
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1940 2501 2591 2929 3160 3233 3275 5417 6836
Size: 561 90 338 231 73 42 2142 1419
Cuts at: 6836 7949 8381 9128 9396 1940
Size: 1113 432 747 268 2668
Fragments arranged by size:
2668 2142 1419 1113 747 561 432 338
268 231 90 73 42
AfeI AGC'GCT
Cuts at: 2808 2808
Size: 10124
AflIII A'CryG_T
Cuts at: 4499 7476 8580 9557 4499
Size: 2977 1104 977 5066
Fragments arranged by size:
5066 2977 1104 977
AgeI A'CCGG_T
Cuts at: 8512 8512
Size: 10124
AhdI GACnn_n'nnGTC
Cuts at: 3854 3854
Size: 10124
AleI CACnn'nnGTG
Cuts at: 4504 4504
Size: 10124
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2771 2972 2771
Size: 201 9923
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 686 718 5154 5186 686
Size: 32 4436 32 5624
Fragments arranged by size:
5624 4436 32 32
AlwNI CAG_nnn'CTG
Cuts at: 229 343 1972 2401 4762 4903 5079 5281 5644
Size: 114 1629 429 2361 141 176 202 363
Cuts at: 5644 6322 6496 7055 7429 8996 229
Size: 678 174 559 374 1567 1357
Fragments arranged by size:
2361 1629 1567 1357 678 559 429 374
363 202 176 174 141 114
ApaI G_GGCC'C
Cuts at: 3534 5563 3534
Size: 2029 8095
ApaLI G'TGCA_C
Cuts at: 496 4595 8894 496
Size: 4099 4299 1726
Fragments arranged by size:
4299 4099 1726
ApoI r'AATT_y
Cuts at: 328 863 1151 3481 3611 7128 9477 9733 328
Size: 535 288 2330 130 3517 2349 256 719
Fragments arranged by size:
3517 2349 2330 719 535 288 256 130
AseI AT'TA_AT
Cuts at: 20 20
Size: 10124
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 10124
AvaI C'yCGr_G
Cuts at: 1814 2384 2767 3590 3839 7144 1814
Size: 570 383 823 249 3305 4794
Fragments arranged by size:
4794 3305 823 570 383 249
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 911 944 911
Size: 33 10091
BamHI G'GATC_C
Cuts at: 543 4860 4890 5517 7149 543
Size: 4317 30 627 1632 3518
Fragments arranged by size:
4317 3518 1632 627 30
BanI G'GyrC_C
Cuts at: 2637 3556 3658 4144 7140 7554 7802 7837 2637
Size: 919 102 486 2996 414 248 35 4924
Fragments arranged by size:
4924 2996 919 486 414 248 102 35
BanII G_rGCy'C
Cuts at: 1268 1928 2420 2774 3534 3646 4573 5563 6236
Size: 660 492 354 760 112 927 990 673
Cuts at: 6236 6245 7138 8168 1268
Size: 9 893 1030 3224
Fragments arranged by size:
3224 1030 990 927 893 760 673 660
492 354 112 9
BbeI G_GCGC'C
Cuts at: 7558 7806 7558
Size: 248 9876
BbsI GAAGACnn'nnnn_
Cuts at: 1739 2285 3740 6596 6920 1739
Size: 546 1455 2856 324 4943
Fragments arranged by size:
4943 2856 1455 546 324
BbvCI CC'TCA_GC
Cuts at: 517 517
Size: 10124
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 283 317 1606 1640 283
Size: 34 1289 34 8767
Fragments arranged by size:
8767 1289 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2988 4750 8022 8783 10124 2988
Size: 1762 3272 761 1341 2988
Fragments arranged by size:
3272 2988 1762 1341 761
BclI T'GATC_A
Cuts at: 609 1041 5942 5955 6776 7644 609
Size: 432 4901 13 821 868 3089
Fragments arranged by size:
4901 3089 868 821 432 13
BfrBI ATG'CAT
Cuts at: 2653 3057 2653
Size: 404 9720
BglI GCCn_nnn'nGGC
Cuts at: 513 1853 1912 2838 5399 513
Size: 1340 59 926 2561 5238
Fragments arranged by size:
5238 2561 1340 926 59
BglII A'GATC_T
Cuts at: 1445 6500 7639 9676 1445
Size: 5055 1139 2037 1893
Fragments arranged by size:
5055 2037 1893 1139
BlpI GC'TnA_GC
Cuts at: 2744 5528 6485 6976 7236 2744
Size: 2784 957 491 260 5632
Fragments arranged by size:
5632 2784 957 491 260
Bme1580I G_kGCm'C
Cuts at: 500 3534 3661 4312 4599 5563 5927 7749 7842
Size: 3034 127 651 287 964 364 1822 93
Cuts at: 7842 8898 500
Size: 1056 1726
Fragments arranged by size:
3034 1822 1726 1056 964 651 364 287
127 93
BmgBI CAC'GTC
Cuts at: 436 4502 6222 436
Size: 4066 1720 4338
Fragments arranged by size:
4338 4066 1720
BmrI ACTGGGnnnn_n'
Cuts at: 394 2055 2432 2769 3007 5089 5465 7336 7417
Size: 1661 377 337 238 2082 376 1871 81
Cuts at: 7417 7752 9896 394
Size: 335 2144 622
Fragments arranged by size:
2144 2082 1871 1661 622 377 376 337
335 238 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3959 6264 8491 3959
Size: 2305 2227 5592
Fragments arranged by size:
5592 2305 2227
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3094 4241 4739 4922 5237 6143 3094
Size: 1147 498 183 315 906 7075
Fragments arranged by size:
7075 1147 906 498 315 183
Bpu10I CC'TnA_GC
Cuts at: 517 758 2231 2454 517
Size: 241 1473 223 8187
Fragments arranged by size:
8187 1473 241 223
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 338 2434 3365 7300 8179 8671 8969 338
Size: 2096 931 3935 879 492 298 1493
Fragments arranged by size:
3935 2096 1493 931 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1972 2491 4474 5089 5443 5460 6159 7090 9828
Size: 519 1983 615 354 17 699 931 2738
Cuts at: 9828 1972
Size: 2268
Fragments arranged by size:
2738 2268 1983 931 699 615 519 354
17
BsaAI yAC'GTr
Cuts at: 2801 7477 2801
Size: 4676 5448
BsaBI GATnn'nnATC
Cuts at: 6 833 2549 4802 7638 9675 6
Size: 827 1716 2253 2836 2037 455
Fragments arranged by size:
2836 2253 2037 1716 827 455
BsaHI Gr'CG_yC
Cuts at: 6895 7555 7803 8505 10001 6895
Size: 660 248 702 1496 7018
Fragments arranged by size:
7018 1496 702 660 248
BsaWI w'CCGG_w
Cuts at: 6264 7313 7834 8512 8786 8933 9344 6264
Size: 1049 521 678 274 147 411 7044
Fragments arranged by size:
7044 1049 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 354 384 5069 5099 5154 5184 5205 5235 5440
Size: 30 4685 30 55 30 21 30 205
Cuts at: 5440 5470 354
Size: 30 5008
Fragments arranged by size:
5008 4685 205 55 30 30 30 30
30 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 161 773 3091 5162 6228 6441 6770 6849 7025
Size: 612 2318 2071 1066 213 329 79 176
Cuts at: 7025 161
Size: 3260
Fragments arranged by size:
3260 2318 2071 1066 612 329 213 176
79
BseYI C'CCAG_C
Cuts at: 2353 4743 5094 5554 6017 6659 7549 8307 8884
Size: 2390 351 460 463 642 890 758 577
Cuts at: 8884 9380 2353
Size: 496 3097
Fragments arranged by size:
3097 2390 890 758 642 577 496 463
460 351
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2312 2417 2823 2967 5630 2312
Size: 105 406 144 2663 6806
Fragments arranged by size:
6806 2663 406 144 105
BsiEI CG_ry'CG
Cuts at: 104 4282 7666 7712 8920 104
Size: 4178 3384 46 1208 1308
Fragments arranged by size:
4178 3384 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 500 2322 2564 4276 4599 7011 7138 7916 8106
Size: 1822 242 1712 323 2412 127 778 190
Cuts at: 8106 8898 500
Size: 792 1726
Fragments arranged by size:
2412 1822 1726 1712 792 778 323 242
190 127
BsmI GAATG_Cn'
Cuts at: 142 550 3626 3728 3840 4409 142
Size: 408 3076 102 112 569 5857
Fragments arranged by size:
5857 3076 569 408 112 102
BsmBI CGTCTCn'nnnn_
Cuts at: 2907 6228 2907
Size: 3321 6803
Bsp1286I G_dGCh'C
Cuts at: 500 1268 1928 2322 2420 2564 2774 3534 3646
Size: 768 660 394 98 144 210 760 112
Cuts at: 3646 3661 4276 4312 4573 4599 5563 5927 6236
Size: 15 615 36 261 26 964 364 309
Cuts at: 6236 6245 7011 7138 7749 7842 7916 8106 8168
Size: 9 766 127 611 93 74 190 62
Cuts at: 8168 8898 500
Size: 730 1726
Fragments arranged by size:
1726 964 768 766 760 730 660 615
611 394 364 309 261 210 190 144
127 112 98 93 74 62 36 26
15 9
BspEI T'CCGG_A
Cuts at: 9344 9344
Size: 10124
BspHI T'CATG_A
Cuts at: 408 3870 4209 4698 9300 408
Size: 3462 339 489 4602 1232
Fragments arranged by size:
4602 3462 1232 489 339
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2409 4853 5375 5751 7174 7388 7690 8071 2409
Size: 2444 522 376 1423 214 302 381 4462
Fragments arranged by size:
4462 2444 1423 522 381 376 302 214
BsrBI CCG'CTC
Cuts at: 50 3020 5313 5325 5331 6347 8416 8470 9954
Size: 2970 2293 12 6 1016 2069 54 1484
Cuts at: 9954 10119 50
Size: 165 55
Fragments arranged by size:
2970 2293 2069 1484 1016 165 55 54
12 6
BsrDI GCAATG_nn'
Cuts at: 1223 3059 4976 8036 1223
Size: 1836 1917 3060 3311
Fragments arranged by size:
3311 3060 1917 1836
BsrFI r'CCGG_y
Cuts at: 8122 8303 8512 8122
Size: 181 209 9734
Fragments arranged by size:
9734 209 181
BsrGI T'GTAC_A
Cuts at: 6864 6864
Size: 10124
BssSI C'ACGA_G
Cuts at: 2177 8395 8753 2177
Size: 6218 358 3548
Fragments arranged by size:
6218 3548 358
BstAPI GCAn_nnn'nTGC
Cuts at: 504 2056 4903 5281 5644 6322 7501 504
Size: 1552 2847 378 363 678 1179 3127
Fragments arranged by size:
3127 2847 1552 1179 678 378 363
BstBI TT'CG_AA
Cuts at: 303 1155 8485 303
Size: 852 7330 1942
Fragments arranged by size:
7330 1942 852
BstEII G'GTnAC_C
Cuts at: 4942 4942
Size: 10124
BstXI CCAn_nnnn'nTGG
Cuts at: 4857 4857
Size: 10124
BstYI r'GATC_y
Cuts at: 1 543 701 1445 2890 2931 3406 4860 4890
Size: 542 158 744 1445 41 475 1454 30
Cuts at: 4890 5517 6500 7052 7149 7616 7639 7974 8220
Size: 627 983 552 97 467 23 335 246
Cuts at: 8220 9232 9318 9330 9676 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1454 1445 1012 983 744 627 552 542
475 467 449 346 335 246 158 97
86 41 30 23 12
BstZ17I GTA'TAC
Cuts at: 188 188
Size: 10124
Bsu36I CC'TnA_GG
Cuts at: 3512 3512
Size: 10124
BtgI C'CryG_G
Cuts at: 2423 3427 4398 5177 6407 2423
Size: 1004 971 779 1230 6140
Fragments arranged by size:
6140 1230 1004 971 779
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5723 8074 8255 8418 5723
Size: 2351 181 163 7429
Fragments arranged by size:
7429 2351 181 163
BtsI GCAGTG_nn'
Cuts at: 4540 4817 5545 5600 7075 7367 4540
Size: 277 728 55 1475 292 7297
Fragments arranged by size:
7297 1475 728 292 277 55
DraI TTT'AAA
Cuts at: 987 3884 7123 987
Size: 2897 3239 3988
Fragments arranged by size:
3988 3239 2897
DrdI GACnn_nn'nnGTC
Cuts at: 4029 5402 7830 8688 4029
Size: 1373 2428 858 5465
Fragments arranged by size:
5465 2428 1373 858
EaeI y'GGCC_r
Cuts at: 4279 5048 5371 5391 7709 7883 8274 8301 9646
Size: 769 323 20 2318 174 391 27 1345
Cuts at: 9646 4279
Size: 4757
Fragments arranged by size:
4757 2318 1345 769 391 323 174 27
20
EagI C'GGCC_G
Cuts at: 4279 7709 4279
Size: 3430 6694
EarI CTCTTCn'nnn_
Cuts at: 638 734 1507 1655 1706 1916 2177 2567 6231
Size: 96 773 148 51 210 261 390 3664
Cuts at: 6231 6717 6855 6930 8147 8357 638
Size: 486 138 75 1217 210 2405
Fragments arranged by size:
3664 2405 1217 773 486 390 261 210
210 148 138 96 75 51
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 8332 8642 8788 9934 8332
Size: 310 146 1146 8522
Fragments arranged by size:
8522 1146 310 146
EcoICRI GAG'CTC
Cuts at: 7136 7136
Size: 10124
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1940 2501 2591 2929 3094 3160 3233 3275 4241
Size: 561 90 338 165 66 73 42 966
Cuts at: 4241 4739 4922 5237 5417 6143 6836 7949 8381
Size: 498 183 315 180 726 693 1113 432
Cuts at: 8381 9128 9396 1940
Size: 747 268 2668
Fragments arranged by size:
2668 1113 966 747 726 693 561 498
432 338 315 268 183 180 165 90
73 66 42
EcoNI CCTnn'n_nnAGG
Cuts at: 3749 5153 6749 6821 3749
Size: 1404 1596 72 7052
Fragments arranged by size:
7052 1596 1404 72
EcoO109I rG'GnC_Cy
Cuts at: 1383 3530 4381 4604 5559 5560 5930 6735 6920
Size: 2147 851 223 955 1 370 805 185
Cuts at: 6920 7263 1383
Size: 343 4244
Fragments arranged by size:
4244 2147 955 851 805 370 343 223
185 1
EcoRI G'AATT_C
Cuts at: 7128 7128
Size: 10124
EcoRV GAT'ATC
Cuts at: 5681 5681
Size: 10124
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 329 1464 2231 2339 4105 8143 329
Size: 1135 767 108 1766 4038 2310
Fragments arranged by size:
4038 2310 1766 1135 767 108
FspI TGC'GCA
Cuts at: 7905 7905
Size: 10124
HaeII r_GCGC'y
Cuts at: 2810 7558 7806 8828 9807 2810
Size: 4748 248 1022 979 3127
Fragments arranged by size:
4748 3127 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 648 680 1252 1284 2761 2793 2882 2914 3189
Size: 32 572 32 1477 32 89 32 275
Cuts at: 3189 3221 3308 3340 3840 3872 4318 4350 4773
Size: 32 87 32 500 32 446 32 423
Cuts at: 4773 4805 4976 5008 5670 5702 6331 6363 6492
Size: 32 171 32 662 32 629 32 129
Cuts at: 6492 6524 6907 6939 7087 7119 7966 7996 7998
Size: 32 383 32 148 32 847 30 2
Cuts at: 7998 8028 648
Size: 30 2744
Fragments arranged by size:
2744 1477 847 662 629 572 500 446
423 383 275 171 148 129 89 87
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 7163 7163
Size: 10124
HindIII A'AGCT_T
Cuts at: 4074 6388 6618 4074
Size: 2314 230 7580
Fragments arranged by size:
7580 2314 230
KasI G'GCGC_C
Cuts at: 7554 7802 7554
Size: 248 9876
KpnI G_GTAC'C
Cuts at: 3560 7144 3560
Size: 3584 6540
MfeI C'AATT_G
Cuts at: 2054 3002 2054
Size: 948 9176
MluI A'CGCG_T
Cuts at: 9557 9557
Size: 10124
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3253 3625 4228 5158 5381 5545 7085 8795 8979
Size: 372 603 930 223 164 1540 1710 184
Cuts at: 8979 3253
Size: 4398
Fragments arranged by size:
4398 1710 1540 930 603 372 223 184
164
MscI TGG'CCA
Cuts at: 5050 7885 5050
Size: 2835 7289
MslI CAynn'nnrTG
Cuts at: 1363 3542 4438 4504 5411 5693 8113 8240 1363
Size: 2179 896 66 907 282 2420 127 3247
Fragments arranged by size:
3247 2420 2179 907 896 282 127 66
MspA1I CmG'CkG
Cuts at: 504 1891 1912 2479 3084 4413 4900 5278 5886
Size: 1387 21 567 605 1329 487 378 608
Cuts at: 5886 6481 7235 7549 7909 8922 9167 504
Size: 595 754 314 360 1013 245 1461
Fragments arranged by size:
1461 1387 1329 1013 754 608 605 595
567 487 378 360 314 245 21
NaeI GCC'GGC
Cuts at: 8305 8305
Size: 10124
NarI GG'CG_CC
Cuts at: 7555 7803 7555
Size: 248 9876
NcoI C'CATG_G
Cuts at: 5177 6407 5177
Size: 1230 8894
NdeI CA'TA_TG
Cuts at: 3819 4167 3819
Size: 348 9776
NgoMIV G'CCGG_C
Cuts at: 8303 8303
Size: 10124
NsiI A_TGCA'T
Cuts at: 2655 3059 2655
Size: 404 9720
NspI r_CATG'y
Cuts at: 7007 7177 7677 8584 7007
Size: 170 500 907 8547
Fragments arranged by size:
8547 907 500 170
PasI CC'CwG_GG
Cuts at: 3989 4305 4573 3989
Size: 316 268 9540
Fragments arranged by size:
9540 316 268
PciI A'CATG_T
Cuts at: 8580 8580
Size: 10124
PflMI CCAn_nnn'nTGG
Cuts at: 4171 4762 6666 4171
Size: 591 1904 7629
Fragments arranged by size:
7629 1904 591
PfoI T'CCnGG_A
Cuts at: 3071 5253 3071
Size: 2182 7942
PmeI GTTT'AAAC
Cuts at: 7123 7123
Size: 10124
PmlI CAC'GTG
Cuts at: 2801 2801
Size: 10124
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5154 5186 9263 9295 5154
Size: 32 4077 32 5983
Fragments arranged by size:
5983 4077 32 32
PpuMI rG'GwC_Cy
Cuts at: 1383 4604 5930 6735 1383
Size: 3221 1326 805 4772
Fragments arranged by size:
4772 3221 1326 805
PshAI GACnn'nnGTC
Cuts at: 3740 3740
Size: 10124
PsiI TTA'TAA
Cuts at: 1568 3310 6953 1568
Size: 1742 3643 4739
Fragments arranged by size:
4739 3643 1742
PspOMI G'GGCC_C
Cuts at: 3530 5559 3530
Size: 2029 8095
PstI C_TGCA'G
Cuts at: 531 1890 1905 4824 4899 5349 5640 5768 6327
Size: 1359 15 2919 75 450 291 128 559
Cuts at: 6327 7171 7856 531
Size: 844 685 2799
Fragments arranged by size:
2919 2799 1359 844 685 559 450 291
128 75 15
PvuI CG_AT'CG
Cuts at: 104 104
Size: 10124
PvuII CAG'CTG
Cuts at: 1891 2479 5278 5886 6481 7549 7909 1891
Size: 588 2799 608 595 1068 360 4106
Fragments arranged by size:
4106 2799 1068 608 595 588 360
RsrII CG'GwC_CG
Cuts at: 8319 8319
Size: 10124
SacI G_AGCT'C
Cuts at: 7138 7138
Size: 10124
SalI G'TCGA_C
Cuts at: 7161 7161
Size: 10124
SanDI GG'GwC_CC
Cuts at: 6735 6735
Size: 10124
SapI GCTCTTCn'nnn_
Cuts at: 1916 2177 8147 8357 1916
Size: 261 5970 210 3683
Fragments arranged by size:
5970 3683 261 210
SbfI CC_TGCA'GG
Cuts at: 7171 7171
Size: 10124
ScaI AGT'ACT
Cuts at: 6984 9641 6984
Size: 2657 7467
SexAI A'CCwGG_T
Cuts at: 953 1864 6141 953
Size: 911 4277 4936
Fragments arranged by size:
4936 4277 911
SfcI C'TryA_G
Cuts at: 33 527 1886 1901 1964 4820 4895 5345 5636
Size: 494 1359 15 63 2856 75 450 291
Cuts at: 5636 5764 6323 7167 7852 8845 9036 33
Size: 128 559 844 685 993 191 1121
Fragments arranged by size:
2856 1359 1121 993 844 685 559 494
450 291 191 128 75 63 15
SfoI GGC'GCC
Cuts at: 7556 7804 7556
Size: 248 9876
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 10124
SmaI CCC'GGG
Cuts at: 1816 7146 1816
Size: 5330 4794
SmlI C'TyrA_G
Cuts at: 353 2384 2413 3380 7279 8194 8686 8948 353
Size: 2031 29 967 3899 915 492 262 1529
Fragments arranged by size:
3899 2031 1529 967 915 492 262 29
SpeI A'CTAG_T
Cuts at: 67 367 67
Size: 300 9824
SphI G_CATG'C
Cuts at: 7177 7677 7177
Size: 500 9624
SspI AAT'ATT
Cuts at: 3828 3828
Size: 10124
StuI AGG'CCT
Cuts at: 351 5579 6195 351
Size: 5228 616 4280
Fragments arranged by size:
5228 4280 616
StyI C'CwwG_G
Cuts at: 135 923 1751 4219 5177 5481 5574 6407 7258
Size: 788 828 2468 958 304 93 833 851
Cuts at: 7258 7612 135
Size: 354 2647
Fragments arranged by size:
2647 2468 958 851 833 828 788 354
304 93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 350 2451 3405 4659 5276 6420 7707 7840 8299
Size: 2101 954 1254 617 1144 1287 133 459
Cuts at: 8299 8508 350
Size: 209 1966
Fragments arranged by size:
2101 1966 1287 1254 1144 954 617 459
209 133
TatI w'GTAC_w
Cuts at: 2440 2467 6864 6982 9639 2440
Size: 27 4397 118 2657 2925
Fragments arranged by size:
4397 2925 2657 118 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2999 3031 5069 5101 5181 5213 9641 9673 2999
Size: 32 2038 32 80 32 4428 32 3450
Fragments arranged by size:
4428 3450 2038 80 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 434 4774 7101 7921 434
Size: 4340 2327 820 2637
Fragments arranged by size:
4340 2637 2327 820
XbaI T'CTAG_A
Cuts at: 6544 7155 6544
Size: 611 9513
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2709 3161 3282 3399 4191 5488 6905 7619 2709
Size: 452 121 117 792 1297 1417 714 5214
Fragments arranged by size:
5214 1417 1297 792 714 452 121 117
XhoI C'TCGA_G
Cuts at: 2384 2384
Size: 10124
XmaI C'CCGG_G
Cuts at: 1814 7144 1814
Size: 5330 4794
XmnI GAAnn'nnTTC
Cuts at: 380 1181 3173 3218 380
Size: 801 1992 45 7286
Fragments arranged by size:
7286 1992 801 45
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII AflII AscI AvrII BmtI BsiWI BssHII ClaI
CspCI DraIII FseI FspAI HpaI NheI NotI NruI
PacI PspXI PsrI SacII SfiI SgrAI SnaBI SrfI
SwaI ZraI