Restriction map for
pF1KA1070
(Circular) (Six-base) MAPSORT of: pF1KA1070.seq Check: 8548 from: 1 to: 5582
pF1KA1070 5582 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AccI GT'mk_AC
Cuts at: 1426 1972 2620 1426
Size: 546 648 4388
Fragments arranged by size:
4388 648 546
Acc65I G'GTAC_C
Cuts at: 1935 2598 1935
Size: 663 4919
AclI AA'CG_TT
Cuts at: 5422 5422
Size: 5582
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 956 1406 3407 3839 4586 4854 205
Size: 751 450 2001 432 747 268 933
Fragments arranged by size:
2001 933 751 747 450 432 268
AflII C'TTAA_G
Cuts at: 864 864
Size: 5582
AflIII A'CryG_T
Cuts at: 2934 4038 5015 2934
Size: 1104 977 3501
Fragments arranged by size:
3501 1104 977
AgeI A'CCGG_T
Cuts at: 3970 3970
Size: 5582
AhdI GACnn_n'nnGTC
Cuts at: 2378 2378
Size: 5582
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1616 1648 1616
Size: 32 5550
AlwNI CAG_nnn'CTG
Cuts at: 2887 4454 2887
Size: 1567 4015
ApaLI G'TGCA_C
Cuts at: 4352 4352
Size: 5582
ApoI r'AATT_y
Cuts at: 323 675 1288 1356 1740 1789 2031 2586 4935
Size: 352 613 68 384 49 242 555 2349
Cuts at: 4935 5191 323
Size: 256 714
Fragments arranged by size:
2349 714 613 555 384 352 256 242
68 49
AseI AT'TA_AT
Cuts at: 20 20
Size: 5582
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5582
AvaI C'yCGr_G
Cuts at: 2602 2602
Size: 5582
BamHI G'GATC_C
Cuts at: 2607 2607
Size: 5582
BanI G'GyrC_C
Cuts at: 184 1506 1935 2598 3012 3260 3295 184
Size: 1322 429 663 414 248 35 2471
Fragments arranged by size:
2471 1322 663 429 414 248 35
BanII G_rGCy'C
Cuts at: 2596 3626 2596
Size: 1030 4552
BbeI G_GCGC'C
Cuts at: 3016 3264 3016
Size: 248 5334
BbsI GAAGACnn'nnnn_
Cuts at: 566 566
Size: 5582
BbvCI CC'TCA_GC
Cuts at: 2249 2249
Size: 5582
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 699 733 760 794 699
Size: 34 27 34 5487
Fragments arranged by size:
5487 34 34 27
BciVI GTATCCnnnnn_n'
Cuts at: 3480 4241 5582 3480
Size: 761 1341 3480
Fragments arranged by size:
3480 1341 761
BclI T'GATC_A
Cuts at: 3102 3102
Size: 5582
BglII A'GATC_T
Cuts at: 3097 5134 3097
Size: 2037 3545
BlpI GC'TnA_GC
Cuts at: 2694 2694
Size: 5582
Bme1580I G_kGCm'C
Cuts at: 189 3207 3300 4356 189
Size: 3018 93 1056 1415
Fragments arranged by size:
3018 1415 1056 93
BmgBI CAC'GTC
Cuts at: 2048 2048
Size: 5582
BmrI ACTGGGnnnn_n'
Cuts at: 1443 1654 2794 2875 3210 5354 1443
Size: 211 1140 81 335 2144 1671
Fragments arranged by size:
2144 1671 1140 335 211 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1258 3949 1258
Size: 2691 2891
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 383 2531 383
Size: 2148 3434
Bpu10I CC'TnA_GC
Cuts at: 2249 2249
Size: 5582
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 780 1763 2758 3637 4129 4427 780
Size: 983 995 879 492 298 1935
Fragments arranged by size:
1935 995 983 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5286 5286
Size: 5582
BsaAI yAC'GTr
Cuts at: 128 1556 2935 128
Size: 1428 1379 2775
Fragments arranged by size:
2775 1428 1379
BsaBI GATnn'nnATC
Cuts at: 6 721 2327 3096 5133 6
Size: 715 1606 769 2037 455
Fragments arranged by size:
2037 1606 769 715 455
BsaHI Gr'CG_yC
Cuts at: 3013 3261 3963 5459 3013
Size: 248 702 1496 3136
Fragments arranged by size:
3136 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2771 3292 3970 4244 4391 4802 2771
Size: 521 678 274 147 411 3551
Fragments arranged by size:
3551 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1616 1646 1847 1877 1616
Size: 30 201 30 5321
Fragments arranged by size:
5321 201 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2232 2421 2232
Size: 189 5393
BseYI C'CCAG_C
Cuts at: 892 1013 3007 3765 4342 4838 892
Size: 121 1994 758 577 496 1636
Fragments arranged by size:
1994 1636 758 577 496 121
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1736 1736
Size: 5582
BsiEI CG_ry'CG
Cuts at: 104 3124 3170 4378 104
Size: 3020 46 1208 1308
Fragments arranged by size:
3020 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2596 3374 3564 4356 2596
Size: 778 190 792 3822
Fragments arranged by size:
3822 792 778 190
BsmI GAATG_Cn'
Cuts at: 1108 1108
Size: 5582
BsmBI CGTCTCn'nnnn_
Cuts at: 1619 1619
Size: 5582
Bsp1286I G_dGCh'C
Cuts at: 189 2596 3207 3300 3374 3564 3626 4356 189
Size: 2407 611 93 74 190 62 730 1415
Fragments arranged by size:
2407 1415 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4802 4802
Size: 5582
BspHI T'CATG_A
Cuts at: 2331 4758 2331
Size: 2427 3155
BspMI ACCTGCnnnn'nnnn_
Cuts at: 920 1041 2632 2846 3148 3529 920
Size: 121 1591 214 302 381 2973
Fragments arranged by size:
2973 1591 381 302 214 121
BsrBI CCG'CTC
Cuts at: 50 3874 3928 5412 5577 50
Size: 3824 54 1484 165 55
Fragments arranged by size:
3824 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 717 1080 1578 3494 717
Size: 363 498 1916 2805
Fragments arranged by size:
2805 1916 498 363
BsrFI r'CCGG_y
Cuts at: 3580 3761 3970 3580
Size: 181 209 5192
Fragments arranged by size:
5192 209 181
BssSI C'ACGA_G
Cuts at: 3853 4211 3853
Size: 358 5224
BstAPI GCAn_nnn'nTGC
Cuts at: 1042 2959 1042
Size: 1917 3665
BstBI TT'CG_AA
Cuts at: 3943 3943
Size: 5582
BstEII G'GTnAC_C
Cuts at: 856 940 1756 2411 856
Size: 84 816 655 4027
Fragments arranged by size:
4027 816 655 84
BstXI CCAn_nnnn'nTGG
Cuts at: 416 416
Size: 5582
BstYI r'GATC_y
Cuts at: 1 883 1527 2607 3074 3097 3432 3678 4690
Size: 882 644 1080 467 23 335 246 1012
Cuts at: 4690 4776 4788 5134 1
Size: 86 12 346 449
Fragments arranged by size:
1080 1012 882 644 467 449 346 335
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 1427 1973 1427
Size: 546 5036
BtgI C'CryG_G
Cuts at: 107 645 1620 107
Size: 538 975 4069
Fragments arranged by size:
4069 975 538
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1328 3532 3713 3876 1328
Size: 2204 181 163 3034
Fragments arranged by size:
3034 2204 181 163
BtsI GCAGTG_nn'
Cuts at: 112 158 2060 2161 2825 112
Size: 46 1902 101 664 2869
Fragments arranged by size:
2869 1902 664 101 46
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 426 461 1047 1082 426
Size: 35 586 35 4926
Fragments arranged by size:
4926 586 35 35
DraI TTT'AAA
Cuts at: 575 2581 575
Size: 2006 3576
DrdI GACnn_nn'nnGTC
Cuts at: 3288 4146 3288
Size: 858 4724
EaeI y'GGCC_r
Cuts at: 130 228 1055 3167 3341 3732 3759 5104 130
Size: 98 827 2112 174 391 27 1345 608
Fragments arranged by size:
2112 1345 827 608 391 174 98 27
EagI C'GGCC_G
Cuts at: 3167 3167
Size: 5582
EarI CTCTTCn'nnn_
Cuts at: 2282 3605 3815 2282
Size: 1323 210 4049
Fragments arranged by size:
4049 1323 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3790 4100 4246 5392 3790
Size: 310 146 1146 3980
Fragments arranged by size:
3980 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2594 2594
Size: 5582
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 383 956 1406 2531 3407 3839 4586 4854
Size: 178 573 450 1125 876 432 747 268
Cuts at: 4854 205
Size: 933
Fragments arranged by size:
1125 933 876 747 573 450 432 268
178
EcoO109I rG'GnC_Cy
Cuts at: 331 619 2721 331
Size: 288 2102 3192
Fragments arranged by size:
3192 2102 288
EcoRI G'AATT_C
Cuts at: 2586 2586
Size: 5582
EcoRV GAT'ATC
Cuts at: 426 1381 426
Size: 955 4627
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 514 1873 3601 514
Size: 1359 1728 2495
Fragments arranged by size:
2495 1728 1359
FspI TGC'GCA
Cuts at: 3363 3363
Size: 5582
HaeII r_GCGC'y
Cuts at: 3016 3264 4286 5265 3016
Size: 248 1022 979 3333
Fragments arranged by size:
3333 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1309 1341 1519 1551 2364 2396 3424 3454 3456
Size: 32 178 32 813 32 1028 30 2
Cuts at: 3456 3486 1309
Size: 30 3405
Fragments arranged by size:
3405 1028 813 178 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 732 909 1137 2621 732
Size: 177 228 1484 3693
Fragments arranged by size:
3693 1484 228 177
HindIII A'AGCT_T
Cuts at: 815 815
Size: 5582
KasI G'GCGC_C
Cuts at: 3012 3260 3012
Size: 248 5334
KpnI G_GTAC'C
Cuts at: 1939 2602 1939
Size: 663 4919
MluI A'CGCG_T
Cuts at: 5015 5015
Size: 5582
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 928 1907 2133 4253 4437 928
Size: 979 226 2120 184 2073
Fragments arranged by size:
2120 2073 979 226 184
MscI TGG'CCA
Cuts at: 132 230 1057 3343 132
Size: 98 827 2286 2371
Fragments arranged by size:
2371 2286 827 98
MslI CAynn'nnrTG
Cuts at: 2336 2354 2452 3571 3698 2336
Size: 18 98 1119 127 4220
Fragments arranged by size:
4220 1119 127 98 18
MspA1I CmG'CkG
Cuts at: 1013 1513 1525 2693 3007 3367 4380 4625 1013
Size: 500 12 1168 314 360 1013 245 1970
Fragments arranged by size:
1970 1168 1013 500 360 314 245 12
NaeI GCC'GGC
Cuts at: 3763 3763
Size: 5582
NarI GG'CG_CC
Cuts at: 3013 3261 3013
Size: 248 5334
NcoI C'CATG_G
Cuts at: 107 645 107
Size: 538 5044
NdeI CA'TA_TG
Cuts at: 360 540 360
Size: 180 5402
NgoMIV G'CCGG_C
Cuts at: 3761 3761
Size: 5582
NspI r_CATG'y
Cuts at: 164 2635 3135 4042 164
Size: 2471 500 907 1704
Fragments arranged by size:
2471 1704 907 500
PciI A'CATG_T
Cuts at: 4038 4038
Size: 5582
PmeI GTTT'AAAC
Cuts at: 2581 2581
Size: 5582
PmlI CAC'GTG
Cuts at: 128 128
Size: 5582
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1616 1648 4721 4753 1616
Size: 32 3073 32 2445
Fragments arranged by size:
3073 2445 32 32
PpuMI rG'GwC_Cy
Cuts at: 619 619
Size: 5582
PstI C_TGCA'G
Cuts at: 2629 3314 2629
Size: 685 4897
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5582
PvuII CAG'CTG
Cuts at: 1013 1513 3007 3367 1013
Size: 500 1494 360 3228
Fragments arranged by size:
3228 1494 500 360
RsrII CG'GwC_CG
Cuts at: 2372 3777 2372
Size: 1405 4177
SacI G_AGCT'C
Cuts at: 2596 2596
Size: 5582
SalI G'TCGA_C
Cuts at: 2619 2619
Size: 5582
SanDI GG'GwC_CC
Cuts at: 619 619
Size: 5582
SapI GCTCTTCn'nnn_
Cuts at: 3605 3815 3605
Size: 210 5372
SbfI CC_TGCA'GG
Cuts at: 2629 2629
Size: 5582
ScaI AGT'ACT
Cuts at: 749 5099 749
Size: 4350 1232
SfcI C'TryA_G
Cuts at: 33 1108 1514 1667 2625 3310 4303 4494 33
Size: 1075 406 153 958 685 993 191 1121
Fragments arranged by size:
1121 1075 993 958 685 406 191 153
SfoI GGC'GCC
Cuts at: 3014 3262 3014
Size: 248 5334
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5582
SmaI CCC'GGG
Cuts at: 2604 2604
Size: 5582
SmlI C'TyrA_G
Cuts at: 759 864 1778 2737 3652 4144 4406 759
Size: 105 914 959 915 492 262 1935
Fragments arranged by size:
1935 959 915 914 492 262 105
SnaBI TAC'GTA
Cuts at: 1556 1556
Size: 5582
SpeI A'CTAG_T
Cuts at: 67 829 67
Size: 762 4820
SphI G_CATG'C
Cuts at: 164 2635 3135 164
Size: 2471 500 2611
Fragments arranged by size:
2611 2471 500
StyI C'CwwG_G
Cuts at: 107 645 1272 2716 3070 107
Size: 538 627 1444 354 2619
Fragments arranged by size:
2619 1444 627 538 354
SwaI ATTT'AAAT
Cuts at: 575 575
Size: 5582
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 172 1491 2454 3165 3298 3757 3966 172
Size: 1319 963 711 133 459 209 1788
Fragments arranged by size:
1788 1319 963 711 459 209 133
TatI w'GTAC_w
Cuts at: 747 763 1641 2198 5097 747
Size: 16 878 557 2899 1232
Fragments arranged by size:
2899 1232 878 557 16
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1483 1515 5099 5131 1483
Size: 32 3584 32 1934
Fragments arranged by size:
3584 1934 32 32
Tth111I GACn'n_nGTC
Cuts at: 3379 3379
Size: 5582
XbaI T'CTAG_A
Cuts at: 2613 2613
Size: 5582
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 140 413 630 1171 3077 140
Size: 273 217 541 1906 2645
Fragments arranged by size:
2645 1906 541 273 217
XmaI C'CCGG_G
Cuts at: 2602 2602
Size: 5582
XmnI GAAnn'nnTTC
Cuts at: 2031 2031
Size: 5582
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PciI PmeI
PmlI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StyI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AleI AlfI ApaI AscI AvrII
BaeI BfrBI BglI BmtI BsiWI BsrGI BssHII Bsu36I
ClaI DraIII EcoNI FseI FspAI HpaI MfeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PshAI
PsiI PspOMI PspXI PsrI SacII SexAI SfiI SgrAI
SrfI SspI StuI XhoI ZraI