Restriction map for
pF1KA1071
(Circular) (Six-base) MAPSORT of: pF1KA1071.seq Check: 9739 from: 1 to: 6365
pF1KA1071 6365 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1160 1160
Size: 6365
AccI GT'mk_AC
Cuts at: 3403 3403
Size: 6365
Acc65I G'GTAC_C
Cuts at: 3381 3381
Size: 6365
AclI AA'CG_TT
Cuts at: 2060 6205 2060
Size: 4145 2220
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 140 817 3107 4190 4622 4975 5637 140
Size: 677 2290 1083 432 353 662 868
Fragments arranged by size:
2290 1083 868 677 662 432 353
AflII C'TTAA_G
Cuts at: 604 604
Size: 6365
AflIII A'CryG_T
Cuts at: 3717 5517 5798 3717
Size: 1800 281 4284
Fragments arranged by size:
4284 1800 281
AgeI A'CCGG_T
Cuts at: 3205 3205
Size: 6365
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1745 1745
Size: 6365
AlwNI CAG_nnn'CTG
Cuts at: 1842 2770 2848 2875 2899 2935 2956 2977 3318
Size: 928 78 27 24 36 21 21 341
Cuts at: 3318 3670 5108 1842
Size: 352 1438 3099
Fragments arranged by size:
3099 1438 928 352 341 78 36 27
24 21 21
ApaI G_GGCC'C
Cuts at: 277 277
Size: 6365
ApaLI G'TGCA_C
Cuts at: 5203 5203
Size: 6365
ApoI r'AATT_y
Cuts at: 114 754 3369 5718 5974 114
Size: 640 2615 2349 256 505
Fragments arranged by size:
2615 2349 640 505 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6365
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6365
AvaI C'yCGr_G
Cuts at: 270 1340 3385 270
Size: 1070 2045 3250
Fragments arranged by size:
3250 2045 1070
AvrII C'CTAG_G
Cuts at: 1156 2577 1156
Size: 1421 4944
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2679 2712 2679
Size: 33 6332
BamHI G'GATC_C
Cuts at: 3390 3390
Size: 6365
BanI G'GyrC_C
Cuts at: 3114 3381 3795 4043 4078 3114
Size: 267 414 248 35 5401
Fragments arranged by size:
5401 414 267 248 35
BanII G_rGCy'C
Cuts at: 277 1347 1410 2096 3379 4409 277
Size: 1070 63 686 1283 1030 2233
Fragments arranged by size:
2233 1283 1070 1030 686 63
BbeI G_GCGC'C
Cuts at: 3799 4047 3799
Size: 248 6117
BbvCI CC'TCA_GC
Cuts at: 2970 3182 2970
Size: 212 6153
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2637 2671 2637
Size: 34 6331
BciVI GTATCCnnnnn_n'
Cuts at: 4263 5319 6365 4263
Size: 1056 1046 4263
Fragments arranged by size:
4263 1056 1046
BclI T'GATC_A
Cuts at: 2390 3885 2390
Size: 1495 4870
BfrBI ATG'CAT
Cuts at: 1620 1620
Size: 6365
BglI GCCn_nnn'nGGC
Cuts at: 800 800
Size: 6365
BglII A'GATC_T
Cuts at: 3880 5917 3880
Size: 2037 4328
BlpI GC'TnA_GC
Cuts at: 1346 3477 1346
Size: 2131 4234
Bme1580I G_kGCm'C
Cuts at: 277 890 3990 4083 5207 277
Size: 613 3100 93 1124 1435
Fragments arranged by size:
3100 1435 1124 613 93
BmrI ACTGGGnnnn_n'
Cuts at: 248 450 3577 3658 3993 6137 248
Size: 202 3127 81 335 2144 476
Fragments arranged by size:
3127 2144 476 335 202 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1446 4732 1446
Size: 3286 3079
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 530 1930 2020 2386 2747 2825 2852 2876 2912
Size: 1400 90 366 361 78 27 24 36
Cuts at: 2912 3008 3023 3059 3131 3149 530
Size: 96 15 36 72 18 3746
Fragments arranged by size:
3746 1400 366 361 96 90 78 72
36 36 27 24 18 15
Bpu10I CC'TnA_GC
Cuts at: 2970 3086 3176 3182 2970
Size: 116 90 6 6153
Fragments arranged by size:
6153 116 90 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 648 1470 3541 4420 5134 5432 648
Size: 822 2071 879 714 298 1581
Fragments arranged by size:
2071 1581 879 822 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 593 1130 6069 593
Size: 537 4939 889
Fragments arranged by size:
4939 889 537
BsaAI yAC'GTr
Cuts at: 3718 3718
Size: 6365
BsaBI GATnn'nnATC
Cuts at: 6 3879 5916 6
Size: 3873 2037 455
Fragments arranged by size:
3873 2037 455
BsaHI Gr'CG_yC
Cuts at: 543 3796 4044 4746 6242 543
Size: 3253 248 702 1496 666
Fragments arranged by size:
3253 1496 702 666 248
BsaWI w'CCGG_w
Cuts at: 1442 1988 3063 3205 3554 4075 4753 5164 5311
Size: 546 1075 142 349 521 678 411 147
Cuts at: 5311 5585 1442
Size: 274 2222
Fragments arranged by size:
2222 1075 678 546 521 411 349 274
147 142
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 681 711 1198 1228 3111 3135 3141 3147 3165
Size: 30 487 30 1883 24 6 6 18
Cuts at: 3165 3177 681
Size: 12 3869
Fragments arranged by size:
3869 1883 487 30 30 24 18 12
6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 389 2125 2182 2311 389
Size: 1736 57 129 4443
Fragments arranged by size:
4443 1736 129 57
BseYI C'CCAG_C
Cuts at: 1409 3790 4548 5213 5621 1409
Size: 2381 758 665 408 2153
Fragments arranged by size:
2381 2153 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2066 2066
Size: 6365
BsiEI CG_ry'CG
Cuts at: 104 3907 3953 5183 104
Size: 3803 46 1230 1286
Fragments arranged by size:
3803 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 699 1982 2096 3379 4157 4347 5207 699
Size: 1283 114 1283 778 190 860 1857
Fragments arranged by size:
1857 1283 1283 860 778 190 114
BsmI GAATG_Cn'
Cuts at: 410 2582 410
Size: 2172 4193
BsmBI CGTCTCn'nnnn_
Cuts at: 1216 1527 1990 2546 2894 1216
Size: 311 463 556 348 4687
Fragments arranged by size:
4687 556 463 348 311
Bsp1286I G_dGCh'C
Cuts at: 277 699 890 1347 1410 1982 2096 3379 3990
Size: 422 191 457 63 572 114 1283 611
Cuts at: 3990 4083 4157 4347 4409 5207 277
Size: 93 74 190 62 798 1435
Fragments arranged by size:
1435 1283 798 611 572 457 422 191
190 114 93 74 63 62
BspEI T'CCGG_A
Cuts at: 1988 1988
Size: 6365
BspHI T'CATG_A
Cuts at: 4797 4797
Size: 6365
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1160 3253 3415 3629 3931 4312 1160
Size: 2093 162 214 302 381 3213
Fragments arranged by size:
3213 2093 381 302 214 162
BsrBI CCG'CTC
Cuts at: 50 4657 4711 6195 6360 50
Size: 4607 54 1484 165 55
Fragments arranged by size:
4607 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 997 4277 997
Size: 3280 3085
BsrFI r'CCGG_y
Cuts at: 3205 4363 4544 3205
Size: 1158 181 5026
Fragments arranged by size:
5026 1158 181
BsrGI T'GTAC_A
Cuts at: 1938 1938
Size: 6365
BssSI C'ACGA_G
Cuts at: 1543 4636 5344 1543
Size: 3093 708 2564
Fragments arranged by size:
3093 2564 708
BstAPI GCAn_nnn'nTGC
Cuts at: 159 2740 3742 159
Size: 2581 1002 2782
Fragments arranged by size:
2782 2581 1002
BstBI TT'CG_AA
Cuts at: 4726 4726
Size: 6365
BstXI CCAn_nnnn'nTGG
Cuts at: 2826 3291 2826
Size: 465 5900
BstYI r'GATC_y
Cuts at: 1 1424 1635 3314 3390 3857 3880 4215 4461
Size: 1423 211 1679 76 467 23 335 246
Cuts at: 4461 4767 4779 4865 5917 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1679 1423 1052 467 449 335 306 246
211 86 76 23 12
BtgI C'CryG_G
Cuts at: 143 2825 143
Size: 2682 3683
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 889 4315 4496 4659 889
Size: 3426 181 163 2595
Fragments arranged by size:
3426 2595 181 163
BtsI GCAGTG_nn'
Cuts at: 267 1784 2780 2795 2801 2897 3608 267
Size: 1517 996 15 6 96 711 3024
Fragments arranged by size:
3024 1517 996 711 96 15 6
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3206 3241 3206
Size: 35 6330
DraI TTT'AAA
Cuts at: 3364 3364
Size: 6365
DrdI GACnn_nn'nnGTC
Cuts at: 2233 4071 5415 2233
Size: 1838 1344 3183
Fragments arranged by size:
3183 1838 1344
EaeI y'GGCC_r
Cuts at: 656 1781 2315 2455 3950 4124 4515 4542 5887
Size: 1125 534 140 1495 174 391 27 1345
Cuts at: 5887 656
Size: 1134
Fragments arranged by size:
1495 1345 1134 1125 534 391 174 140
27
EagI C'GGCC_G
Cuts at: 3950 3950
Size: 6365
EarI CTCTTCn'nnn_
Cuts at: 1874 4388 4598 1874
Size: 2514 210 3641
Fragments arranged by size:
3641 2514 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4573 5315 5461 6175 4573
Size: 742 146 714 4763
Fragments arranged by size:
4763 742 714 146
EcoICRI GAG'CTC
Cuts at: 2094 3377 2094
Size: 1283 5082
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 140 530 817 1930 2020 2386 2747 2825 2852
Size: 390 287 1113 90 366 361 78 27
Cuts at: 2852 2876 2912 3008 3023 3059 3107 3131 3149
Size: 24 36 96 15 36 48 24 18
Cuts at: 3149 4190 4622 4975 5637 140
Size: 1041 432 353 662 868
Fragments arranged by size:
1113 1041 868 662 432 390 366 361
353 287 96 90 78 48 36 36
27 24 24 18 15
EcoO109I rG'GnC_Cy
Cuts at: 274 817 1061 1569 1839 1932 3504 274
Size: 543 244 508 270 93 1572 3135
Fragments arranged by size:
3135 1572 543 508 270 244 93
EcoRI G'AATT_C
Cuts at: 754 3369 754
Size: 2615 3750
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 190 793 4384 190
Size: 603 3591 2171
Fragments arranged by size:
3591 2171 603
FspI TGC'GCA
Cuts at: 4146 4146
Size: 6365
HaeII r_GCGC'y
Cuts at: 3799 4047 5277 6048 3799
Size: 248 1230 771 4116
Fragments arranged by size:
4116 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 933 965 1632 1664 1805 1837 2109 2141 2428
Size: 32 667 32 141 32 272 32 287
Cuts at: 2428 2460 3234 3266 4207 4237 4239 4269 933
Size: 32 774 32 941 30 2 30 3029
Fragments arranged by size:
3029 941 774 667 287 272 141 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1908 3404 1908
Size: 1496 4869
KasI G'GCGC_C
Cuts at: 3795 4043 3795
Size: 248 6117
KpnI G_GTAC'C
Cuts at: 3385 3385
Size: 6365
MluI A'CGCG_T
Cuts at: 5798 5798
Size: 6365
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 468 1442 3166 3178 3184 5124 5308 468
Size: 974 1724 12 6 1940 184 1525
Fragments arranged by size:
1940 1724 1525 974 184 12 6
MscI TGG'CCA
Cuts at: 658 1783 2317 4126 658
Size: 1125 534 1809 2897
Fragments arranged by size:
2897 1809 1125 534
MslI CAynn'nnrTG
Cuts at: 482 584 2824 4354 4481 482
Size: 102 2240 1530 127 2366
Fragments arranged by size:
2366 2240 1530 127 102
MspA1I CmG'CkG
Cuts at: 393 558 2442 2737 2872 2905 2932 2953 2974
Size: 165 1884 295 135 33 27 21 21
Cuts at: 2974 3028 3082 3169 3476 3790 4150 4934 5179
Size: 54 54 87 307 314 360 784 245
Cuts at: 5179 393
Size: 1579
Fragments arranged by size:
1884 1579 784 360 314 307 295 245
165 135 87 54 54 33 27 21
21
NaeI GCC'GGC
Cuts at: 4546 4546
Size: 6365
NarI GG'CG_CC
Cuts at: 3796 4044 3796
Size: 248 6117
NcoI C'CATG_G
Cuts at: 2825 2825
Size: 6365
NdeI CA'TA_TG
Cuts at: 1533 1533
Size: 6365
NgoMIV G'CCGG_C
Cuts at: 4544 4544
Size: 6365
NsiI A_TGCA'T
Cuts at: 1622 1622
Size: 6365
NspI r_CATG'y
Cuts at: 481 619 849 1958 2454 3418 3918 5521 481
Size: 138 230 1109 496 964 500 1603 1325
Fragments arranged by size:
1603 1325 1109 964 500 496 230 138
PasI CC'CwG_GG
Cuts at: 348 348
Size: 6365
PciI A'CATG_T
Cuts at: 5517 5517
Size: 6365
PflMI CCAn_nnn'nTGG
Cuts at: 1416 1416
Size: 6365
PfoI T'CCnGG_A
Cuts at: 567 2413 3210 567
Size: 1846 797 3722
Fragments arranged by size:
3722 1846 797
PmeI GTTT'AAAC
Cuts at: 3364 3364
Size: 6365
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4811 4843 4811
Size: 32 6333
PpuMI rG'GwC_Cy
Cuts at: 1061 1061
Size: 6365
PspOMI G'GGCC_C
Cuts at: 273 273
Size: 6365
PstI C_TGCA'G
Cuts at: 1496 2695 2736 3412 4097 1496
Size: 1199 41 676 685 3764
Fragments arranged by size:
3764 1199 685 676 41
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6365
PvuII CAG'CTG
Cuts at: 393 2442 2737 2872 2932 2953 2974 3028 3169
Size: 2049 295 135 60 21 21 54 141
Cuts at: 3169 3790 4150 393
Size: 621 360 2608
Fragments arranged by size:
2608 2049 621 360 295 141 135 60
54 21 21
RsrII CG'GwC_CG
Cuts at: 4560 4560
Size: 6365
SacI G_AGCT'C
Cuts at: 2096 3379 2096
Size: 1283 5082
SalI G'TCGA_C
Cuts at: 3402 3402
Size: 6365
SapI GCTCTTCn'nnn_
Cuts at: 1874 4388 4598 1874
Size: 2514 210 3641
Fragments arranged by size:
3641 2514 210
SbfI CC_TGCA'GG
Cuts at: 3412 3412
Size: 6365
ScaI AGT'ACT
Cuts at: 460 2399 5882 460
Size: 1939 3483 943
Fragments arranged by size:
3483 1939 943
SfcI C'TryA_G
Cuts at: 33 860 894 1492 2691 2732 3408 4093 5061
Size: 827 34 598 1199 41 676 685 968
Cuts at: 5061 5252 33
Size: 191 1146
Fragments arranged by size:
1199 1146 968 827 685 676 598 191
41 34
SfoI GGC'GCC
Cuts at: 3797 4045 3797
Size: 248 6117
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6365
SmaI CCC'GGG
Cuts at: 272 3387 272
Size: 3115 3250
SmlI C'TyrA_G
Cuts at: 604 627 1449 3520 4435 5149 5411 604
Size: 23 822 2071 915 714 262 1558
Fragments arranged by size:
2071 1558 915 822 714 262 23
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6365
SphI G_CATG'C
Cuts at: 481 849 2454 3418 3918 481
Size: 368 1605 964 500 2928
Fragments arranged by size:
2928 1605 964 500 368
SspI AAT'ATT
Cuts at: 2161 2161
Size: 6365
StyI C'CwwG_G
Cuts at: 443 768 1118 1156 1859 2577 2825 3499 3853
Size: 325 350 38 703 718 248 674 354
Cuts at: 3853 443
Size: 2955
Fragments arranged by size:
2955 718 703 674 354 350 325 248
38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2581 3948 4081 4540 4749 2581
Size: 1367 133 459 209 4197
Fragments arranged by size:
4197 1367 459 209 133
TatI w'GTAC_w
Cuts at: 458 1938 2397 5880 458
Size: 1480 459 3483 943
Fragments arranged by size:
3483 1480 943 459
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5882 5914 5882
Size: 32 6333
Tth111I GACn'n_nGTC
Cuts at: 4162 4162
Size: 6365
XbaI T'CTAG_A
Cuts at: 1650 2274 3396 1650
Size: 624 1122 4619
Fragments arranged by size:
4619 1122 624
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2707 3315 3860 2707
Size: 608 545 5212
Fragments arranged by size:
5212 608 545
XmaI C'CCGG_G
Cuts at: 270 3385 270
Size: 3115 3250
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BanII BbeI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AhdI AleI AloI AscI BbsI BmgBI
BmtI BsiWI BssHII BstEII BstZ17I Bsu36I ClaI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NheI
NotI NruI PacI PmlI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI XmnI ZraI