Restriction map for
pF1KA1080
(Circular) (Six-base) MAPSORT of: pF1KA1080.seq Check: 8597 from: 1 to: 4952
pF1KA1080 4952 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 252 252
Size: 4952
AccI GT'mk_AC
Cuts at: 1990 1990
Size: 4952
Acc65I G'GTAC_C
Cuts at: 1968 1968
Size: 4952
AclI AA'CG_TT
Cuts at: 4792 4792
Size: 4952
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 498 592 712 2777 3209 3562 4224 498
Size: 94 120 2065 432 353 662 1226
Fragments arranged by size:
2065 1226 662 432 353 120 94
AflIII A'CryG_T
Cuts at: 2304 4104 4385 2304
Size: 1800 281 2871
Fragments arranged by size:
2871 1800 281
AgeI A'CCGG_T
Cuts at: 740 740
Size: 4952
AlwNI CAG_nnn'CTG
Cuts at: 570 1224 1656 1815 2257 3695 570
Size: 654 432 159 442 1438 1827
Fragments arranged by size:
1827 1438 654 442 432 159
ApaI G_GGCC'C
Cuts at: 172 172
Size: 4952
ApaLI G'TGCA_C
Cuts at: 3790 3790
Size: 4952
ApoI r'AATT_y
Cuts at: 249 415 753 992 1356 1601 1956 4305 4561
Size: 166 338 239 364 245 355 2349 256
Cuts at: 4561 249
Size: 640
Fragments arranged by size:
2349 640 364 355 338 256 245 239
166
AseI AT'TA_AT
Cuts at: 20 20
Size: 4952
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4952
AvaI C'yCGr_G
Cuts at: 165 718 1972 165
Size: 553 1254 3145
Fragments arranged by size:
3145 1254 553
BamHI G'GATC_C
Cuts at: 1977 1977
Size: 4952
BanI G'GyrC_C
Cuts at: 1200 1637 1968 2382 2630 2665 1200
Size: 437 331 414 248 35 3487
Fragments arranged by size:
3487 437 414 331 248 35
BanII G_rGCy'C
Cuts at: 172 655 1632 1966 2996 172
Size: 483 977 334 1030 2128
Fragments arranged by size:
2128 1030 977 483 334
BbeI G_GCGC'C
Cuts at: 2386 2634 2386
Size: 248 4704
BbsI GAAGACnn'nnnn_
Cuts at: 545 1147 545
Size: 602 4350
BciVI GTATCCnnnnn_n'
Cuts at: 2850 3906 4952 2850
Size: 1056 1046 2850
Fragments arranged by size:
2850 1056 1046
BclI T'GATC_A
Cuts at: 437 2472 437
Size: 2035 2917
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4952
BglII A'GATC_T
Cuts at: 2467 4504 2467
Size: 2037 2915
BlpI GC'TnA_GC
Cuts at: 853 2064 853
Size: 1211 3741
Bme1580I G_kGCm'C
Cuts at: 172 1396 1640 2577 2670 3794 172
Size: 1224 244 937 93 1124 1330
Fragments arranged by size:
1330 1224 1124 937 244 93
BmrI ACTGGGnnnn_n'
Cuts at: 2164 2245 2580 4724 2164
Size: 81 335 2144 2392
Fragments arranged by size:
2392 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1697 3319 1697
Size: 1622 3330
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 385 982 1092 1293 1442 1778 205
Size: 180 597 110 201 149 336 3379
Fragments arranged by size:
3379 597 336 201 180 149 110
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1508 2128 3007 3721 4019 1508
Size: 620 879 714 298 2441
Fragments arranged by size:
2441 879 714 620 298
BsaI GGTCTCn'nnnn_
Cuts at: 4656 4656
Size: 4952
BsaAI yAC'GTr
Cuts at: 2305 2305
Size: 4952
BsaBI GATnn'nnATC
Cuts at: 6 2466 4503 6
Size: 2460 2037 455
Fragments arranged by size:
2460 2037 455
BsaHI Gr'CG_yC
Cuts at: 2383 2631 3333 4829 2383
Size: 248 702 1496 2506
Fragments arranged by size:
2506 1496 702 248
BsaWI w'CCGG_w
Cuts at: 534 740 947 1586 2141 2662 3340 3751 3898
Size: 206 207 639 555 521 678 411 147
Cuts at: 3898 4172 534
Size: 274 1314
Fragments arranged by size:
1314 678 639 555 521 411 274 207
206 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 775 805 775
Size: 30 4922
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1003 1296 1360 1003
Size: 293 64 4595
Fragments arranged by size:
4595 293 64
BseYI C'CCAG_C
Cuts at: 1555 1685 2377 3135 3800 4208 1555
Size: 130 692 758 665 408 2299
Fragments arranged by size:
2299 758 692 665 408 130
BsiEI CG_ry'CG
Cuts at: 104 119 2494 2540 3770 104
Size: 15 2375 46 1230 1286
Fragments arranged by size:
2375 1286 1230 46 15
BsiHKAI G_wGCw'C
Cuts at: 655 1678 1966 2744 2934 3794 655
Size: 1023 288 778 190 860 1813
Fragments arranged by size:
1813 1023 860 778 288 190
BsmI GAATG_Cn'
Cuts at: 1774 1774
Size: 4952
BsmBI CGTCTCn'nnnn_
Cuts at: 548 548
Size: 4952
Bsp1286I G_dGCh'C
Cuts at: 172 655 1396 1632 1640 1678 1966 2577 2670
Size: 483 741 236 8 38 288 611 93
Cuts at: 2670 2744 2934 2996 3794 172
Size: 74 190 62 798 1330
Fragments arranged by size:
1330 798 741 611 483 288 236 190
93 74 62 38 8
BspEI T'CCGG_A
Cuts at: 534 534
Size: 4952
BspHI T'CATG_A
Cuts at: 3384 3384
Size: 4952
BspMI ACCTGCnnnn'nnnn_
Cuts at: 252 894 1153 1188 2002 2216 2518 2899 252
Size: 642 259 35 814 214 302 381 2305
Fragments arranged by size:
2305 814 642 381 302 259 214 35
BsrBI CCG'CTC
Cuts at: 50 3244 3298 4782 4947 50
Size: 3194 54 1484 165 55
Fragments arranged by size:
3194 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2864 2864
Size: 4952
BsrFI r'CCGG_y
Cuts at: 740 1749 2950 3131 740
Size: 1009 1201 181 2561
Fragments arranged by size:
2561 1201 1009 181
BsrGI T'GTAC_A
Cuts at: 1034 1034
Size: 4952
BssSI C'ACGA_G
Cuts at: 3223 3931 3223
Size: 708 4244
BstAPI GCAn_nnn'nTGC
Cuts at: 849 2329 849
Size: 1480 3472
BstBI TT'CG_AA
Cuts at: 3313 3313
Size: 4952
BstEII G'GTnAC_C
Cuts at: 1059 1059
Size: 4952
BstXI CCAn_nnnn'nTGG
Cuts at: 641 1089 641
Size: 448 4504
BstYI r'GATC_y
Cuts at: 1 317 1977 2444 2467 2802 3048 3354 3366
Size: 316 1660 467 23 335 246 306 12
Cuts at: 3366 3452 4504 1
Size: 86 1052 449
Fragments arranged by size:
1660 1052 467 449 335 316 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 119 107
Size: 12 4940
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2902 3083 3246 2902
Size: 181 163 4608
Fragments arranged by size:
4608 181 163
BtsI GCAGTG_nn'
Cuts at: 2195 2195
Size: 4952
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1860 1895 1860
Size: 35 4917
DraI TTT'AAA
Cuts at: 1951 1951
Size: 4952
DrdI GACnn_nn'nnGTC
Cuts at: 1102 1930 2658 4002 1102
Size: 828 728 1344 2052
Fragments arranged by size:
2052 1344 828 728
EaeI y'GGCC_r
Cuts at: 410 2537 2711 3102 3129 4474 410
Size: 2127 174 391 27 1345 888
Fragments arranged by size:
2127 1345 888 391 174 27
EagI C'GGCC_G
Cuts at: 2537 2537
Size: 4952
EarI CTCTTCn'nnn_
Cuts at: 489 517 2975 3185 489
Size: 28 2458 210 2256
Fragments arranged by size:
2458 2256 210 28
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3160 3902 4048 4762 3160
Size: 742 146 714 3350
Fragments arranged by size:
3350 742 714 146
EcoICRI GAG'CTC
Cuts at: 653 1964 653
Size: 1311 3641
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 385 498 592 712 982 1092 1293 1442
Size: 180 113 94 120 270 110 201 149
Cuts at: 1442 1778 2777 3209 3562 4224 205
Size: 336 999 432 353 662 933
Fragments arranged by size:
999 933 662 432 353 336 270 201
180 149 120 113 110 94
EcoNI CCTnn'n_nnAGG
Cuts at: 1256 1256
Size: 4952
EcoO109I rG'GnC_Cy
Cuts at: 160 467 723 879 897 1221 2091 160
Size: 307 256 156 18 324 870 3021
Fragments arranged by size:
3021 870 324 307 256 156 18
EcoRI G'AATT_C
Cuts at: 1601 1956 1601
Size: 355 4597
EcoRV GAT'ATC
Cuts at: 1701 1805 1701
Size: 104 4848
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2971 2971
Size: 4952
FspI TGC'GCA
Cuts at: 1184 2733 1184
Size: 1549 3403
HaeII r_GCGC'y
Cuts at: 2386 2634 3864 4635 2386
Size: 248 1230 771 2703
Fragments arranged by size:
2703 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 309 341 775 807 1357 1389 2794 2824 2826
Size: 32 434 32 550 32 1405 30 2
Cuts at: 2826 2856 309
Size: 30 2405
Fragments arranged by size:
2405 1405 550 434 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1991 1991
Size: 4952
HindIII A'AGCT_T
Cuts at: 685 1765 685
Size: 1080 3872
KasI G'GCGC_C
Cuts at: 2382 2630 2382
Size: 248 4704
KpnI G_GTAC'C
Cuts at: 1972 1972
Size: 4952
MluI A'CGCG_T
Cuts at: 4385 4385
Size: 4952
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 203 499 3711 3895 203
Size: 296 3212 184 1260
Fragments arranged by size:
3212 1260 296 184
MscI TGG'CCA
Cuts at: 412 2713 412
Size: 2301 2651
MslI CAynn'nnrTG
Cuts at: 2941 3068 2941
Size: 127 4825
MspA1I CmG'CkG
Cuts at: 121 1798 2063 2377 2737 3521 3766 121
Size: 1677 265 314 360 784 245 1307
Fragments arranged by size:
1677 1307 784 360 314 265 245
NaeI GCC'GGC
Cuts at: 1751 3133 1751
Size: 1382 3570
NarI GG'CG_CC
Cuts at: 2383 2631 2383
Size: 248 4704
NcoI C'CATG_G
Cuts at: 107 107
Size: 4952
NgoMIV G'CCGG_C
Cuts at: 1749 3131 1749
Size: 1382 3570
NspI r_CATG'y
Cuts at: 222 295 861 2005 2505 4108 222
Size: 73 566 1144 500 1603 1066
Fragments arranged by size:
1603 1144 1066 566 500 73
PasI CC'CwG_GG
Cuts at: 156 1633 156
Size: 1477 3475
PciI A'CATG_T
Cuts at: 4104 4104
Size: 4952
PflMI CCAn_nnn'nTGG
Cuts at: 1327 1327
Size: 4952
PmeI GTTT'AAAC
Cuts at: 1951 1951
Size: 4952
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3398 3430 3398
Size: 32 4920
PpuMI rG'GwC_Cy
Cuts at: 160 467 723 1221 160
Size: 307 256 498 3891
Fragments arranged by size:
3891 498 307 256
PsiI TTA'TAA
Cuts at: 589 589
Size: 4952
PspOMI G'GGCC_C
Cuts at: 168 168
Size: 4952
PstI C_TGCA'G
Cuts at: 845 905 1350 1748 1999 2684 845
Size: 60 445 398 251 685 3113
Fragments arranged by size:
3113 685 445 398 251 60
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4952
PvuII CAG'CTG
Cuts at: 1798 2377 2737 1798
Size: 579 360 4013
Fragments arranged by size:
4013 579 360
RsrII CG'GwC_CG
Cuts at: 3147 3147
Size: 4952
SacI G_AGCT'C
Cuts at: 655 1966 655
Size: 1311 3641
SacII CC_GC'GG
Cuts at: 122 122
Size: 4952
SalI G'TCGA_C
Cuts at: 1989 1989
Size: 4952
SanDI GG'GwC_CC
Cuts at: 160 160
Size: 4952
SapI GCTCTTCn'nnn_
Cuts at: 2975 3185 2975
Size: 210 4742
SbfI CC_TGCA'GG
Cuts at: 905 1999 905
Size: 1094 3858
ScaI AGT'ACT
Cuts at: 4469 4469
Size: 4952
SexAI A'CCwGG_T
Cuts at: 1440 1440
Size: 4952
SfcI C'TryA_G
Cuts at: 33 841 901 1346 1744 1995 2680 3648 3839
Size: 808 60 445 398 251 685 968 191
Cuts at: 3839 33
Size: 1146
Fragments arranged by size:
1146 968 808 685 445 398 251 191
60
SfoI GGC'GCC
Cuts at: 2384 2632 2384
Size: 248 4704
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4952
SmaI CCC'GGG
Cuts at: 167 1974 167
Size: 1807 3145
SmlI C'TyrA_G
Cuts at: 1523 2107 3022 3736 3998 1523
Size: 584 915 714 262 2477
Fragments arranged by size:
2477 915 714 584 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4952
SphI G_CATG'C
Cuts at: 2005 2505 2005
Size: 500 4452
StyI C'CwwG_G
Cuts at: 107 1020 1232 1427 1875 2086 2440 107
Size: 913 212 195 448 211 354 2619
Fragments arranged by size:
2619 913 448 354 212 211 195
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1033 1655 2535 2668 3127 3336 1033
Size: 622 880 133 459 209 2649
Fragments arranged by size:
2649 880 622 459 209 133
TatI w'GTAC_w
Cuts at: 1034 4467 1034
Size: 3433 1519
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1061 1093 4469 4501 1061
Size: 32 3376 32 1512
Fragments arranged by size:
3376 1512 32 32
Tth111I GACn'n_nGTC
Cuts at: 2749 2749
Size: 4952
XbaI T'CTAG_A
Cuts at: 1983 1983
Size: 4952
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 638 1466 2447 638
Size: 828 981 3143
Fragments arranged by size:
3143 981 828
XmaI C'CCGG_G
Cuts at: 165 1972 165
Size: 1807 3145
XmnI GAAnn'nnTTC
Cuts at: 1594 1915 1594
Size: 321 4631
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PmeI PpiI PpuMI PsiI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BsgI BsiWI BssHII BstZ17I Bsu36I ClaI DraIII FseI
FspAI HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PfoI PmlI PshAI PspXI PsrI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI