Restriction map for
pF1KA1081
(Circular) (Six-base) MAPSORT of: pF1KA1081.seq Check: 5962 from: 1 to: 6089
pF1KA1081 6089 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AatII G_ACGT'C
Cuts at: 399 399
Size: 6089
AccI GT'mk_AC
Cuts at: 3127 3127
Size: 6089
Acc65I G'GTAC_C
Cuts at: 3105 3105
Size: 6089
AclI AA'CG_TT
Cuts at: 5929 5929
Size: 6089
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 601 709 1691 1735 2443 2938 3914 4346 5093
Size: 108 982 44 708 495 976 432 747
Cuts at: 5093 5361 601
Size: 268 1329
Fragments arranged by size:
1329 982 976 747 708 495 432 268
108 44
AflII C'TTAA_G
Cuts at: 2110 2950 2110
Size: 840 5249
AflIII A'CryG_T
Cuts at: 1715 3441 4545 5522 1715
Size: 1726 1104 977 2282
Fragments arranged by size:
2282 1726 1104 977
AgeI A'CCGG_T
Cuts at: 4477 4477
Size: 6089
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1589 1589
Size: 6089
AlwNI CAG_nnn'CTG
Cuts at: 1329 1446 2739 3394 4961 1329
Size: 117 1293 655 1567 2457
Fragments arranged by size:
2457 1567 1293 655 117
ApaLI G'TGCA_C
Cuts at: 2456 4859 2456
Size: 2403 3686
ApoI r'AATT_y
Cuts at: 1277 1319 1408 1655 3093 5442 5698 1277
Size: 42 89 247 1438 2349 256 1668
Fragments arranged by size:
2349 1668 1438 256 247 89 42
AseI AT'TA_AT
Cuts at: 20 20
Size: 6089
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6089
AvaI C'yCGr_G
Cuts at: 1019 3109 1019
Size: 2090 3999
BamHI G'GATC_C
Cuts at: 818 3114 818
Size: 2296 3793
BanI G'GyrC_C
Cuts at: 3105 3519 3767 3802 3105
Size: 414 248 35 5392
Fragments arranged by size:
5392 414 248 35
BanII G_rGCy'C
Cuts at: 160 682 705 3103 4133 160
Size: 522 23 2398 1030 2116
Fragments arranged by size:
2398 2116 1030 522 23
BbeI G_GCGC'C
Cuts at: 3523 3771 3523
Size: 248 5841
BbsI GAAGACnn'nnnn_
Cuts at: 1097 2899 1097
Size: 1802 4287
BciVI GTATCCnnnnn_n'
Cuts at: 3987 4748 6089 3987
Size: 761 1341 3987
Fragments arranged by size:
3987 1341 761
BclI T'GATC_A
Cuts at: 3609 3609
Size: 6089
BfrBI ATG'CAT
Cuts at: 1200 1200
Size: 6089
BglII A'GATC_T
Cuts at: 1980 3604 5641 1980
Size: 1624 2037 2428
Fragments arranged by size:
2428 2037 1624
BlpI GC'TnA_GC
Cuts at: 923 3201 923
Size: 2278 3811
Bme1580I G_kGCm'C
Cuts at: 123 2460 3714 3807 4863 123
Size: 2337 1254 93 1056 1349
Fragments arranged by size:
2337 1349 1254 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 3301 3382 3717 5861 3301
Size: 81 335 2144 3529
Fragments arranged by size:
3529 2144 335 81
BmtI G_CTAG'C
Cuts at: 452 2617 452
Size: 2165 3924
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4456 4456
Size: 6089
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 696 787 1712 2827 696
Size: 91 925 1115 3958
Fragments arranged by size:
3958 1115 925 91
Bpu10I CC'TnA_GC
Cuts at: 1559 1559
Size: 6089
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1693 2367 3265 4144 4636 4934 1693
Size: 674 898 879 492 298 2848
Fragments arranged by size:
2848 898 879 674 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1298 5793 1298
Size: 4495 1594
BsaAI yAC'GTr
Cuts at: 3442 3442
Size: 6089
BsaBI GATnn'nnATC
Cuts at: 6 790 3603 5640 6
Size: 784 2813 2037 455
Fragments arranged by size:
2813 2037 784 455
BsaHI Gr'CG_yC
Cuts at: 396 2852 3520 3768 4470 5966 396
Size: 2456 668 248 702 1496 519
Fragments arranged by size:
2456 1496 702 668 519 248
BsaWI w'CCGG_w
Cuts at: 3278 3799 4477 4751 4898 5309 3278
Size: 521 678 274 147 411 4058
Fragments arranged by size:
4058 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2863 2893 3031 3061 2863
Size: 30 138 30 5891
Fragments arranged by size:
5891 138 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 913 1207 1894 2161 2485 2674 3079 3082 913
Size: 294 687 267 324 189 405 3 3920
Fragments arranged by size:
3920 687 405 324 294 267 189 3
BseYI C'CCAG_C
Cuts at: 2729 3514 4272 4849 5345 2729
Size: 785 758 577 496 3473
Fragments arranged by size:
3473 785 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 3631 3677 4885 104
Size: 3527 46 1208 1308
Fragments arranged by size:
3527 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2460 3103 3881 4071 4863 2460
Size: 643 778 190 792 3686
Fragments arranged by size:
3686 792 778 643 190
BsmI GAATG_Cn'
Cuts at: 1221 1221
Size: 6089
Bsp1286I G_dGCh'C
Cuts at: 123 160 682 705 2460 3103 3714 3807 3881
Size: 37 522 23 1755 643 611 93 74
Cuts at: 3881 4071 4133 4863 123
Size: 190 62 730 1349
Fragments arranged by size:
1755 1349 730 643 611 522 190 93
74 62 37 23
BspEI T'CCGG_A
Cuts at: 5309 5309
Size: 6089
BspHI T'CATG_A
Cuts at: 5265 5265
Size: 6089
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3139 3353 3655 4036 3139
Size: 214 302 381 5192
Fragments arranged by size:
5192 381 302 214
BsrBI CCG'CTC
Cuts at: 50 125 933 1734 1902 2601 4381 4435 5919
Size: 75 808 801 168 699 1780 54 1484
Cuts at: 5919 6084 50
Size: 165 55
Fragments arranged by size:
1780 1484 808 801 699 168 165 75
55 54
BsrDI GCAATG_nn'
Cuts at: 4001 4001
Size: 6089
BsrFI r'CCGG_y
Cuts at: 4087 4268 4477 4087
Size: 181 209 5699
Fragments arranged by size:
5699 209 181
BsrGI T'GTAC_A
Cuts at: 759 2583 759
Size: 1824 4265
BssSI C'ACGA_G
Cuts at: 4360 4718 4360
Size: 358 5731
BstAPI GCAn_nnn'nTGC
Cuts at: 3466 3466
Size: 6089
BstBI TT'CG_AA
Cuts at: 960 4450 960
Size: 3490 2599
BstEII G'GTnAC_C
Cuts at: 199 199
Size: 6089
BstXI CCAn_nnnn'nTGG
Cuts at: 160 160
Size: 6089
BstYI r'GATC_y
Cuts at: 1 564 818 1980 2052 3114 3581 3604 3939
Size: 563 254 1162 72 1062 467 23 335
Cuts at: 3939 4185 5197 5283 5295 5641 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1162 1062 1012 563 467 449 346 335
254 246 86 72 23 12
Bsu36I CC'TnA_GG
Cuts at: 2085 2085
Size: 6089
BtgI C'CryG_G
Cuts at: 2675 2675
Size: 6089
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4039 4220 4383 4039
Size: 181 163 5745
Fragments arranged by size:
5745 181 163
BtsI GCAGTG_nn'
Cuts at: 246 261 459 1859 3332 246
Size: 15 198 1400 1473 3003
Fragments arranged by size:
3003 1473 1400 198 15
DraI TTT'AAA
Cuts at: 293 3088 293
Size: 2795 3294
DrdI GACnn_nn'nnGTC
Cuts at: 3795 4653 3795
Size: 858 5231
EaeI y'GGCC_r
Cuts at: 381 1428 2672 2984 3674 3848 4239 4266 5611
Size: 1047 1244 312 690 174 391 27 1345
Cuts at: 5611 381
Size: 859
Fragments arranged by size:
1345 1244 1047 859 690 391 312 174
27
EagI C'GGCC_G
Cuts at: 3674 3674
Size: 6089
EarI CTCTTCn'nnn_
Cuts at: 1307 1613 1667 2446 2549 2831 4112 4322 1307
Size: 306 54 779 103 282 1281 210 3074
Fragments arranged by size:
3074 1281 779 306 282 210 103 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4297 4607 4753 5899 4297
Size: 310 146 1146 4487
Fragments arranged by size:
4487 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3101 3101
Size: 6089
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 601 696 709 787 1691 1712 1735 2443 2827
Size: 95 13 78 904 21 23 708 384
Cuts at: 2827 2938 3914 4346 5093 5361 601
Size: 111 976 432 747 268 1329
Fragments arranged by size:
1329 976 904 747 708 432 384 268
111 95 78 23 21 13
EcoNI CCTnn'n_nnAGG
Cuts at: 538 1312 538
Size: 774 5315
EcoO109I rG'GnC_Cy
Cuts at: 828 1884 3228 828
Size: 1056 1344 3689
Fragments arranged by size:
3689 1344 1056
EcoRI G'AATT_C
Cuts at: 3093 3093
Size: 6089
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1566 1798 2041 2842 4108 1566
Size: 232 243 801 1266 3547
Fragments arranged by size:
3547 1266 801 243 232
FspI TGC'GCA
Cuts at: 3870 3870
Size: 6089
HaeII r_GCGC'y
Cuts at: 3523 3771 4793 5772 3523
Size: 248 1022 979 3840
Fragments arranged by size:
3840 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1752 1784 2863 2895 2952 2984 3931 3961 3963
Size: 32 1079 32 57 32 947 30 2
Cuts at: 3963 3993 1752
Size: 30 3848
Fragments arranged by size:
3848 1079 947 57 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 3128 3128
Size: 6089
HindIII A'AGCT_T
Cuts at: 719 1039 719
Size: 320 5769
KasI G'GCGC_C
Cuts at: 3519 3767 3519
Size: 248 5841
KpnI G_GTAC'C
Cuts at: 3109 3109
Size: 6089
MfeI C'AATT_G
Cuts at: 1907 2170 1907
Size: 263 5826
MluI A'CGCG_T
Cuts at: 5522 5522
Size: 6089
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 225 1700 2348 4760 4944 225
Size: 1475 648 2412 184 1370
Fragments arranged by size:
2412 1475 1370 648 184
MscI TGG'CCA
Cuts at: 1430 2674 3850 1430
Size: 1244 1176 3669
Fragments arranged by size:
3669 1244 1176
MslI CAynn'nnrTG
Cuts at: 341 559 784 1534 4078 4205 341
Size: 218 225 750 2544 127 2225
Fragments arranged by size:
2544 2225 750 225 218 127
MspA1I CmG'CkG
Cuts at: 579 840 3200 3514 3874 4887 5132 579
Size: 261 2360 314 360 1013 245 1536
Fragments arranged by size:
2360 1536 1013 360 314 261 245
NaeI GCC'GGC
Cuts at: 4270 4270
Size: 6089
NarI GG'CG_CC
Cuts at: 3520 3768 3520
Size: 248 5841
NcoI C'CATG_G
Cuts at: 2675 2675
Size: 6089
NgoMIV G'CCGG_C
Cuts at: 4268 4268
Size: 6089
NheI G'CTAG_C
Cuts at: 448 2613 448
Size: 2165 3924
NsiI A_TGCA'T
Cuts at: 1202 1202
Size: 6089
NspI r_CATG'y
Cuts at: 783 1719 2068 3142 3642 4549 783
Size: 936 349 1074 500 907 2323
Fragments arranged by size:
2323 1074 936 907 500 349
PasI CC'CwG_GG
Cuts at: 160 160
Size: 6089
PciI A'CATG_T
Cuts at: 1715 4545 1715
Size: 2830 3259
PfoI T'CCnGG_A
Cuts at: 803 803
Size: 6089
PmeI GTTT'AAAC
Cuts at: 3088 3088
Size: 6089
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5228 5260 5228
Size: 32 6057
PpuMI rG'GwC_Cy
Cuts at: 828 828
Size: 6089
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 201 233 201
Size: 32 6057
PstI C_TGCA'G
Cuts at: 572 1481 1586 2598 3136 3821 572
Size: 909 105 1012 538 685 2840
Fragments arranged by size:
2840 1012 909 685 538 105
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6089
PvuII CAG'CTG
Cuts at: 579 840 3514 3874 579
Size: 261 2674 360 2794
Fragments arranged by size:
2794 2674 360 261
RsrII CG'GwC_CG
Cuts at: 4284 4284
Size: 6089
SacI G_AGCT'C
Cuts at: 3103 3103
Size: 6089
SalI G'TCGA_C
Cuts at: 3126 3126
Size: 6089
SapI GCTCTTCn'nnn_
Cuts at: 2549 2831 4112 4322 2549
Size: 282 1281 210 4316
Fragments arranged by size:
4316 1281 282 210
SbfI CC_TGCA'GG
Cuts at: 3136 3136
Size: 6089
ScaI AGT'ACT
Cuts at: 1353 5606 1353
Size: 4253 1836
SexAI A'CCwGG_T
Cuts at: 2257 2257
Size: 6089
SfcI C'TryA_G
Cuts at: 33 568 1477 1582 2518 2594 3132 3817 4810
Size: 535 909 105 936 76 538 685 993
Cuts at: 4810 5001 33
Size: 191 1121
Fragments arranged by size:
1121 993 936 909 685 538 535 191
105 76
SfoI GGC'GCC
Cuts at: 3521 3769 3521
Size: 248 5841
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6089
SmaI CCC'GGG
Cuts at: 3111 3111
Size: 6089
SmlI C'TyrA_G
Cuts at: 1708 2110 2382 2950 3244 4159 4651 4913 1708
Size: 402 272 568 294 915 492 262 2884
Fragments arranged by size:
2884 915 568 492 402 294 272 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6089
SphI G_CATG'C
Cuts at: 2068 3142 3642 2068
Size: 1074 500 4515
Fragments arranged by size:
4515 1074 500
StyI C'CwwG_G
Cuts at: 1076 2675 3223 3577 1076
Size: 1599 548 354 3588
Fragments arranged by size:
3588 1599 548 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 186 2243 2743 3672 3805 4264 4473 186
Size: 2057 500 929 133 459 209 1802
Fragments arranged by size:
2057 1802 929 500 459 209 133
TatI w'GTAC_w
Cuts at: 749 759 1351 2583 5604 749
Size: 10 592 1232 3021 1234
Fragments arranged by size:
3021 1234 1232 592 10
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2998 3030 3031 3063 5606 5638 2998
Size: 32 1 32 2543 32 3449
Fragments arranged by size:
3449 2543 32 32 32 1
Tth111I GACn'n_nGTC
Cuts at: 3886 3886
Size: 6089
XbaI T'CTAG_A
Cuts at: 3120 3120
Size: 6089
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3584 3584
Size: 6089
XmaI C'CCGG_G
Cuts at: 3109 3109
Size: 6089
XmnI GAAnn'nnTTC
Cuts at: 1769 2825 1769
Size: 1056 5033
ZraI GAC'GTC
Cuts at: 397 397
Size: 6089
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NsiI NspI PasI PciI PfoI
PmeI PpiI PpuMI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AhdI AleI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BglI BmgBI BsgI BsiWI BsmBI
BssHII BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI
HpaI NdeI NotI NruI PacI PflMI PmlI PshAI
PsiI PspOMI PspXI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI