Restriction map for
pF1KA1086
(Circular) (Six-base) MAPSORT of: pF1KA1086.seq Check: 6820 from: 1 to: 5810
pF1KA1086 5810 bp
With 182 enzymes: SgfI *
April 24, 2009 13:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 577 2245 577
Size: 1668 4142
AccI GT'mk_AC
Cuts at: 2848 2848
Size: 5810
Acc65I G'GTAC_C
Cuts at: 2826 2826
Size: 5810
AclI AA'CG_TT
Cuts at: 5650 5650
Size: 5810
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2150 3635 4067 4420 5082 2150
Size: 1485 432 353 662 2878
Fragments arranged by size:
2878 1485 662 432 353
AfeI AGC'GCT
Cuts at: 1160 1160
Size: 5810
AflIII A'CryG_T
Cuts at: 608 3162 4962 5243 608
Size: 2554 1800 281 1175
Fragments arranged by size:
2554 1800 1175 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1242 1242
Size: 5810
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1345 1377 1345
Size: 32 5778
AlwNI CAG_nnn'CTG
Cuts at: 280 684 1683 2073 3115 4553 280
Size: 404 999 390 1042 1438 1537
Fragments arranged by size:
1537 1438 1042 999 404 390
ApaI G_GGCC'C
Cuts at: 782 1342 1677 1848 2409 2468 2476 2540 2715
Size: 560 335 171 561 59 8 64 175
Cuts at: 2715 782
Size: 3877
Fragments arranged by size:
3877 561 560 335 175 171 64 59
8
ApaLI G'TGCA_C
Cuts at: 4648 4648
Size: 5810
ApoI r'AATT_y
Cuts at: 2814 5163 5419 2814
Size: 2349 256 3205
Fragments arranged by size:
3205 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5810
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5810
AvaI C'yCGr_G
Cuts at: 477 514 666 1066 1096 1146 1729 1937 2284
Size: 37 152 400 30 50 583 208 347
Cuts at: 2284 2327 2476 2539 2581 2830 477
Size: 43 149 63 42 249 3457
Fragments arranged by size:
3457 583 400 347 249 208 152 149
63 50 43 42 37 30
BamHI G'GATC_C
Cuts at: 1006 2550 2835 1006
Size: 1544 285 3981
Fragments arranged by size:
3981 1544 285
BanI G'GyrC_C
Cuts at: 435 585 1460 1565 2826 3240 3488 3523 435
Size: 150 875 105 1261 414 248 35 2722
Fragments arranged by size:
2722 1261 875 414 248 150 105 35
BanII G_rGCy'C
Cuts at: 218 782 922 1093 1342 1517 1530 1677 1808
Size: 564 140 171 249 175 13 147 131
Cuts at: 1808 1848 2165 2272 2409 2468 2476 2540 2546
Size: 40 317 107 137 59 8 64 6
Cuts at: 2546 2588 2715 2801 2824 3854 218
Size: 42 127 86 23 1030 2174
Fragments arranged by size:
2174 1030 564 317 249 175 171 147
140 137 131 127 107 86 64 59
42 40 23 13 8 6
BbeI G_GCGC'C
Cuts at: 439 3244 3492 439
Size: 2805 248 2757
Fragments arranged by size:
2805 2757 248
BbsI GAAGACnn'nnnn_
Cuts at: 901 1017 2615 901
Size: 116 1598 4096
Fragments arranged by size:
4096 1598 116
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1422 1456 1422
Size: 34 5776
BciVI GTATCCnnnnn_n'
Cuts at: 157 475 3708 4764 5810 157
Size: 318 3233 1056 1046 157
Fragments arranged by size:
3233 1056 1046 318 157
BclI T'GATC_A
Cuts at: 3330 3330
Size: 5810
BglI GCCn_nnn'nGGC
Cuts at: 401 583 991 1186 1734 2786 401
Size: 182 408 195 548 1052 3425
Fragments arranged by size:
3425 1052 548 408 195 182
BglII A'GATC_T
Cuts at: 3325 5362 3325
Size: 2037 3773
BlpI GC'TnA_GC
Cuts at: 419 2922 419
Size: 2503 3307
Bme1580I G_kGCm'C
Cuts at: 782 1342 1677 1848 2396 2409 2468 2476 2540
Size: 560 335 171 548 13 59 8 64
Cuts at: 2540 2715 3435 3528 4652 782
Size: 175 720 93 1124 1940
Fragments arranged by size:
1940 1124 720 560 548 335 175 171
93 64 59 13 8
BmgBI CAC'GTC
Cuts at: 1118 1647 1764 2613 1118
Size: 529 117 849 4315
Fragments arranged by size:
4315 849 529 117
BmrI ACTGGGnnnn_n'
Cuts at: 1054 1230 1680 3022 3103 3438 5582 1054
Size: 176 450 1342 81 335 2144 1282
Fragments arranged by size:
2144 1342 1282 450 335 176 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2136 2232 2815 4177 2136
Size: 96 583 1362 3769
Fragments arranged by size:
3769 1362 583 96
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 629 775 1561 1606 1645 1708 2071 2521
Size: 319 146 786 45 39 63 363 450
Cuts at: 2521 2530 310
Size: 9 3590
Fragments arranged by size:
3590 786 450 363 319 146 63 45
39 9
Bpu10I CC'TnA_GC
Cuts at: 711 1208 2266 711
Size: 497 1058 4255
Fragments arranged by size:
4255 1058 497
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1834 2986 3865 4579 4877 1834
Size: 1152 879 714 298 2767
Fragments arranged by size:
2767 1152 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 759 1164 2121 2558 5514 759
Size: 405 957 437 2956 1055
Fragments arranged by size:
2956 1055 957 437 405
BsaAI yAC'GTr
Cuts at: 1446 3163 1446
Size: 1717 4093
BsaBI GATnn'nnATC
Cuts at: 6 2359 3324 5361 6
Size: 2353 965 2037 455
Fragments arranged by size:
2353 2037 965 455
BsaHI Gr'CG_yC
Cuts at: 436 506 983 1744 1952 3241 3489 4191 5687
Size: 70 477 761 208 1289 248 702 1496
Cuts at: 5687 436
Size: 559
Fragments arranged by size:
1496 1289 761 702 559 477 248 208
70
BsaWI w'CCGG_w
Cuts at: 451 1475 2720 2999 3520 4198 4609 4756 5030
Size: 1024 1245 279 521 678 411 147 274
Cuts at: 5030 451
Size: 1231
Fragments arranged by size:
1245 1231 1024 678 521 411 279 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1345 1375 1345
Size: 30 5780
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1369 1558 1591 1629 1642 1645 1891 1975 2109
Size: 189 33 38 13 3 246 84 134
Cuts at: 2109 2139 2173 2251 2767 1369
Size: 30 34 78 516 4412
Fragments arranged by size:
4412 516 246 189 134 84 78 38
34 33 30 13 3
BseYI C'CCAG_C
Cuts at: 138 391 751 1132 1516 1676 2066 2461 2627
Size: 253 360 381 384 160 390 395 166
Cuts at: 2627 2706 3235 3993 4658 5066 138
Size: 79 529 758 665 408 882
Fragments arranged by size:
882 758 665 529 408 395 390 384
381 360 253 166 160 79
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 555 925 952 954 1018 1058 1426 1431 1837
Size: 370 27 2 64 40 368 5 406
Cuts at: 1837 555
Size: 4528
Fragments arranged by size:
4528 406 370 368 64 40 27 5
2
BsiEI CG_ry'CG
Cuts at: 104 1741 1758 3352 3398 4628 104
Size: 1637 17 1594 46 1230 1286
Fragments arranged by size:
1637 1594 1286 1230 46 17
BsiHKAI G_wGCw'C
Cuts at: 469 1281 1808 2824 3602 3792 4652 469
Size: 812 527 1016 778 190 860 1627
Fragments arranged by size:
1627 1016 860 812 778 527 190
BsmI GAATG_Cn'
Cuts at: 2648 2648
Size: 5810
Bsp1286I G_dGCh'C
Cuts at: 218 469 782 922 1093 1281 1342 1517 1530
Size: 251 313 140 171 188 61 175 13
Cuts at: 1530 1677 1808 1848 2165 2272 2396 2409 2468
Size: 147 131 40 317 107 124 13 59
Cuts at: 2468 2476 2540 2546 2588 2715 2801 2824 3435
Size: 8 64 6 42 127 86 23 611
Cuts at: 3435 3528 3602 3792 3854 4652 218
Size: 93 74 190 62 798 1376
Fragments arranged by size:
1376 798 611 317 313 251 190 188
175 171 147 140 131 127 124 107
93 86 74 64 62 61 59 42
40 23 13 13 8 6
BspEI T'CCGG_A
Cuts at: 451 2720 451
Size: 2269 3541
BspHI T'CATG_A
Cuts at: 533 4242 533
Size: 3709 2101
BspMI ACCTGCnnnn'nnnn_
Cuts at: 577 1448 2163 2245 2860 3074 3376 3757 577
Size: 871 715 82 615 214 302 381 2630
Fragments arranged by size:
2630 871 715 615 381 302 214 82
BsrBI CCG'CTC
Cuts at: 50 1581 1842 2604 4102 4156 5640 5805 50
Size: 1531 261 762 1498 54 1484 165 55
Fragments arranged by size:
1531 1498 1484 762 261 165 55 54
BsrDI GCAATG_nn'
Cuts at: 945 1502 3722 945
Size: 557 2220 3033
Fragments arranged by size:
3033 2220 557
BsrFI r'CCGG_y
Cuts at: 1332 1463 1740 1757 2384 2702 2780 3808 3989
Size: 131 277 17 627 318 78 1028 181
Cuts at: 3989 1332
Size: 3153
Fragments arranged by size:
3153 1028 627 318 277 181 131 78
17
BssSI C'ACGA_G
Cuts at: 2800 4081 4789 2800
Size: 1281 708 3821
Fragments arranged by size:
3821 1281 708
BstAPI GCAn_nnn'nTGC
Cuts at: 684 1403 1467 2049 2637 3187 684
Size: 719 64 582 588 550 3307
Fragments arranged by size:
3307 719 588 582 550 64
BstBI TT'CG_AA
Cuts at: 4171 4171
Size: 5810
BstEII G'GTnAC_C
Cuts at: 2094 2175 2094
Size: 81 5729
BstXI CCAn_nnnn'nTGG
Cuts at: 237 237
Size: 5810
BstYI r'GATC_y
Cuts at: 1 1006 2550 2682 2835 3302 3325 3660 3906
Size: 1005 1544 132 153 467 23 335 246
Cuts at: 3906 4212 4224 4310 5362 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1544 1052 1005 467 449 335 306 246
153 132 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2243 2371 2243
Size: 128 5682
BtgI C'CryG_G
Cuts at: 107 224 236 399 494 1175 1310 1508 1793
Size: 117 12 163 95 681 135 198 285
Cuts at: 1793 2423 107
Size: 630 3494
Fragments arranged by size:
3494 681 630 285 198 163 135 117
95 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3760 3941 4104 3760
Size: 181 163 5466
Fragments arranged by size:
5466 181 163
BtsI GCAGTG_nn'
Cuts at: 1392 2460 3053 1392
Size: 1068 593 4149
Fragments arranged by size:
4149 1068 593
DraI TTT'AAA
Cuts at: 2809 2809
Size: 5810
DrdI GACnn_nn'nnGTC
Cuts at: 1762 3516 4860 1762
Size: 1754 1344 2712
Fragments arranged by size:
2712 1754 1344
EaeI y'GGCC_r
Cuts at: 180 227 357 1079 1163 1738 1811 1871 2294
Size: 47 130 722 84 575 73 60 423
Cuts at: 2294 2510 3395 3569 3960 3987 5332 180
Size: 216 885 174 391 27 1345 658
Fragments arranged by size:
1345 885 722 658 575 423 391 216
174 130 84 73 60 47 27
EagI C'GGCC_G
Cuts at: 1738 3395 1738
Size: 1657 4153
EarI CTCTTCn'nnn_
Cuts at: 1895 2719 3833 4043 1895
Size: 824 1114 210 3662
Fragments arranged by size:
3662 1114 824 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 994 1588 2804 4018 4760 4906 5620 994
Size: 594 1216 1214 742 146 714 1184
Fragments arranged by size:
1216 1214 1184 742 714 594 146
EcoICRI GAG'CTC
Cuts at: 1806 2822 1806
Size: 1016 4794
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 629 775 1561 1606 1645 1708 2071 2150
Size: 319 146 786 45 39 63 363 79
Cuts at: 2150 2521 2530 3635 4067 4420 5082 310
Size: 371 9 1105 432 353 662 1038
Fragments arranged by size:
1105 1038 786 662 432 371 363 353
319 146 79 63 45 39 9
EcoNI CCTnn'n_nnAGG
Cuts at: 1149 1286 1573 1149
Size: 137 287 5386
Fragments arranged by size:
5386 287 137
EcoO109I rG'GnC_Cy
Cuts at: 368 670 778 1201 1270 1437 1536 1845 1941
Size: 302 108 423 69 167 99 309 96
Cuts at: 1941 2211 2367 2376 2405 2406 2465 2473 2670
Size: 270 156 9 29 1 59 8 197
Cuts at: 2670 2711 2737 2949 368
Size: 41 26 212 3229
Fragments arranged by size:
3229 423 309 302 270 212 197 167
156 108 99 96 69 59 41 29
26 9 8 1
EcoRI G'AATT_C
Cuts at: 2814 2814
Size: 5810
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3829 3829
Size: 5810
FspI TGC'GCA
Cuts at: 1328 3591 1328
Size: 2263 3547
HaeII r_GCGC'y
Cuts at: 439 1162 1602 1718 2627 3244 3492 4722 5493
Size: 723 440 116 909 617 248 1230 771
Cuts at: 5493 439
Size: 756
Fragments arranged by size:
1230 909 771 756 723 617 440 248
116
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1952 1984 3652 3682 3684 3714 1952
Size: 32 1668 30 2 30 4048
Fragments arranged by size:
4048 1668 32 30 30 2
HincII GTy'rAC
Cuts at: 2849 2849
Size: 5810
KasI G'GCGC_C
Cuts at: 435 3240 3488 435
Size: 2805 248 2757
Fragments arranged by size:
2805 2757 248
KpnI G_GTAC'C
Cuts at: 2830 2830
Size: 5810
MluI A'CGCG_T
Cuts at: 608 5243 608
Size: 4635 1175
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1886 2147 4569 4753 1886
Size: 261 2422 184 2943
Fragments arranged by size:
2943 2422 261 184
MscI TGG'CCA
Cuts at: 1081 1165 2512 3571 1081
Size: 84 1347 1059 3320
Fragments arranged by size:
3320 1347 1059 84
MslI CAynn'nnrTG
Cuts at: 235 3799 3926 235
Size: 3564 127 2119
Fragments arranged by size:
3564 2119 127
MspA1I CmG'CkG
Cuts at: 142 280 625 684 824 942 1312 1563 1593
Size: 138 345 59 140 118 370 251 30
Cuts at: 1593 2073 2336 2921 3235 3595 4379 4624 142
Size: 480 263 585 314 360 784 245 1328
Fragments arranged by size:
1328 784 585 480 370 360 345 314
263 251 245 140 138 118 59 30
NaeI GCC'GGC
Cuts at: 1742 2386 2782 3991 1742
Size: 644 396 1209 3561
Fragments arranged by size:
3561 1209 644 396
NarI GG'CG_CC
Cuts at: 436 3241 3489 436
Size: 2805 248 2757
Fragments arranged by size:
2805 2757 248
NcoI C'CATG_G
Cuts at: 107 236 399 2423 107
Size: 129 163 2024 3494
Fragments arranged by size:
3494 2024 163 129
NgoMIV G'CCGG_C
Cuts at: 1740 2384 2780 3989 1740
Size: 644 396 1209 3561
Fragments arranged by size:
3561 1209 644 396
NspI r_CATG'y
Cuts at: 2863 3363 4966 2863
Size: 500 1603 3707
Fragments arranged by size:
3707 1603 500
PasI CC'CwG_GG
Cuts at: 725 2469 725
Size: 1744 4066
PciI A'CATG_T
Cuts at: 4962 4962
Size: 5810
PfoI T'CCnGG_A
Cuts at: 1272 1538 2498 2546 1272
Size: 266 960 48 4536
Fragments arranged by size:
4536 960 266 48
PmeI GTTT'AAAC
Cuts at: 2809 2809
Size: 5810
PmlI CAC'GTG
Cuts at: 1446 1446
Size: 5810
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1345 1377 4256 4288 1345
Size: 32 2879 32 2867
Fragments arranged by size:
2879 2867 32 32
PpuMI rG'GwC_Cy
Cuts at: 368 670 1270 1437 1536 1941 2211 2367 2376
Size: 302 600 167 99 405 270 156 9
Cuts at: 2376 2670 2737 368
Size: 294 67 3441
Fragments arranged by size:
3441 600 405 302 294 270 167 156
99 67 9
PshAI GACnn'nnGTC
Cuts at: 505 1939 505
Size: 1434 4376
PspOMI G'GGCC_C
Cuts at: 778 1338 1673 1844 2405 2464 2472 2536 2711
Size: 560 335 171 561 59 8 64 175
Cuts at: 2711 778
Size: 3877
Fragments arranged by size:
3877 561 560 335 175 171 64 59
8
PspXI vC'TCGA_Gb
Cuts at: 1066 1146 2284 2327 2581 1066
Size: 80 1138 43 254 4295
Fragments arranged by size:
4295 1138 254 80 43
PstI C_TGCA'G
Cuts at: 708 1231 1472 2345 2857 3542 708
Size: 523 241 873 512 685 2976
Fragments arranged by size:
2976 873 685 523 512 241
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5810
PvuII CAG'CTG
Cuts at: 142 280 684 824 942 1593 2073 3235 3595
Size: 138 404 140 118 651 480 1162 360
Cuts at: 3595 142
Size: 2357
Fragments arranged by size:
2357 1162 651 480 404 360 140 138
118
RsrII CG'GwC_CG
Cuts at: 4005 4005
Size: 5810
SacI G_AGCT'C
Cuts at: 1808 2824 1808
Size: 1016 4794
SacII CC_GC'GG
Cuts at: 1313 1313
Size: 5810
SalI G'TCGA_C
Cuts at: 2847 2847
Size: 5810
SanDI GG'GwC_CC
Cuts at: 368 670 1270 368
Size: 302 600 4908
Fragments arranged by size:
4908 600 302
SapI GCTCTTCn'nnn_
Cuts at: 3833 4043 3833
Size: 210 5600
SbfI CC_TGCA'GG
Cuts at: 708 2857 708
Size: 2149 3661
ScaI AGT'ACT
Cuts at: 5327 5327
Size: 5810
SfcI C'TryA_G
Cuts at: 33 704 1227 1468 2341 2853 3538 4506 4697
Size: 671 523 241 873 512 685 968 191
Cuts at: 4697 33
Size: 1146
Fragments arranged by size:
1146 968 873 685 671 523 512 241
191
SfiI GGCCn_nnn'nGGCC
Cuts at: 1186 1186
Size: 5810
SfoI GGC'GCC
Cuts at: 437 3242 3490 437
Size: 2805 248 2757
Fragments arranged by size:
2805 2757 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5810
SmaI CCC'GGG
Cuts at: 668 1098 1731 2478 2541 2832 668
Size: 430 633 747 63 291 3646
Fragments arranged by size:
3646 747 633 430 291 63
SmlI C'TyrA_G
Cuts at: 1066 1146 1849 2284 2327 2581 2965 3880 4594
Size: 80 703 435 43 254 384 915 714
Cuts at: 4594 4856 1066
Size: 262 2020
Fragments arranged by size:
2020 915 714 703 435 384 262 254
80 43
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5810
SphI G_CATG'C
Cuts at: 2863 3363 2863
Size: 500 5310
SrfI GCCC'GGGC
Cuts at: 1731 2541 1731
Size: 810 5000
StuI AGG'CCT
Cuts at: 1189 1982 1189
Size: 793 5017
StyI C'CwwG_G
Cuts at: 107 236 399 770 1287 2423 2944 3298 107
Size: 129 163 371 517 1136 521 354 2619
Fragments arranged by size:
2619 1136 521 517 371 354 163 129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2113 3393 3526 3985 4194 2113
Size: 1280 133 459 209 3729
Fragments arranged by size:
3729 1280 459 209 133
TatI w'GTAC_w
Cuts at: 617 5325 617
Size: 4708 1102
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5327 5359 5327
Size: 32 5778
Tth111I GACn'n_nGTC
Cuts at: 3607 3607
Size: 5810
XbaI T'CTAG_A
Cuts at: 2841 2841
Size: 5810
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1173 2430 2703 3305 1173
Size: 1257 273 602 3678
Fragments arranged by size:
3678 1257 602 273
XhoI C'TCGA_G
Cuts at: 1066 1146 2284 2327 2581 1066
Size: 80 1138 43 254 4295
Fragments arranged by size:
4295 1138 254 80 43
XmaI C'CCGG_G
Cuts at: 666 1096 1729 2476 2539 2830 666
Size: 430 633 747 63 291 3646
Fragments arranged by size:
3646 747 633 430 291 63
XmnI GAAnn'nnTTC
Cuts at: 1024 1024
Size: 5810
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PfoI PmeI PmlI
PpiI PpuMI PshAI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
SrfI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AflII AgeI AhdI AleI AscI AvrII BaeI
BbvCI BfrBI BmtI BsiWI BsmBI BsrGI BssHII BstZ17I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PflMI
PsiI PsrI SexAI SgrAI SnaBI SspI SwaI ZraI