Restriction map for
pF1KA1090
(Circular) (Six-base) MAPSORT of: pF1KA1090.seq Check: 6118 from: 1 to: 7208
pF1KA1090 7208 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2233 3228 2233
Size: 995 6213
AccI GT'mk_AC
Cuts at: 4246 4246
Size: 7208
Acc65I G'GTAC_C
Cuts at: 4224 4224
Size: 7208
AclI AA'CG_TT
Cuts at: 770 7048 770
Size: 6278 930
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 677 1526 1670 1745 1916 1918 2075 3013 3023
Size: 849 144 75 171 2 157 938 10
Cuts at: 3023 4029 5033 5465 5818 6480 677
Size: 1006 1004 432 353 662 1405
Fragments arranged by size:
1405 1006 1004 938 849 662 432 353
171 157 144 75 10 2
AfeI AGC'GCT
Cuts at: 2293 2293
Size: 7208
AflIII A'CryG_T
Cuts at: 3965 4560 6360 6641 3965
Size: 595 1800 281 4532
Fragments arranged by size:
4532 1800 595 281
AhdI GACnn_n'nnGTC
Cuts at: 4065 4065
Size: 7208
AleI CACnn'nnGTG
Cuts at: 2396 3586 2396
Size: 1190 6018
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1553 2161 2529 2817 1553
Size: 608 368 288 5944
Fragments arranged by size:
5944 608 368 288
AlwNI CAG_nnn'CTG
Cuts at: 1393 4513 5951 1393
Size: 3120 1438 2650
Fragments arranged by size:
3120 2650 1438
ApaI G_GGCC'C
Cuts at: 1068 1068
Size: 7208
ApaLI G'TGCA_C
Cuts at: 881 6046 881
Size: 5165 2043
ApoI r'AATT_y
Cuts at: 112 2317 2857 3367 4212 6561 6817 112
Size: 2205 540 510 845 2349 256 503
Fragments arranged by size:
2349 2205 845 540 510 503 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7208
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7208
AvaI C'yCGr_G
Cuts at: 359 2001 3041 3712 4228 359
Size: 1642 1040 671 516 3339
Fragments arranged by size:
3339 1642 1040 671 516
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 874 907 2042 2075 874
Size: 33 1135 33 6007
Fragments arranged by size:
6007 1135 33 33
BamHI G'GATC_C
Cuts at: 4233 4233
Size: 7208
BanI G'GyrC_C
Cuts at: 177 1008 4224 4638 4886 4921 177
Size: 831 3216 414 248 35 2464
Fragments arranged by size:
3216 2464 831 414 248 35
BanII G_rGCy'C
Cuts at: 1068 2015 4222 5252 1068
Size: 947 2207 1030 3024
Fragments arranged by size:
3024 2207 1030 947
BbeI G_GCGC'C
Cuts at: 4642 4890 4642
Size: 248 6960
BbsI GAAGACnn'nnnn_
Cuts at: 214 1441 1485 1661 1765 2970 214
Size: 1227 44 176 104 1205 4452
Fragments arranged by size:
4452 1227 1205 176 104 44
BbvCI CC'TCA_GC
Cuts at: 250 1730 250
Size: 1480 5728
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1756 1790 1826 1860 1756
Size: 34 36 34 7104
Fragments arranged by size:
7104 36 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1694 5106 6162 7208 1694
Size: 3412 1056 1046 1694
Fragments arranged by size:
3412 1694 1056 1046
BclI T'GATC_A
Cuts at: 4728 4728
Size: 7208
BfrBI ATG'CAT
Cuts at: 1885 1885
Size: 7208
BglI GCCn_nnn'nGGC
Cuts at: 1006 1192 1633 1834 1006
Size: 186 441 201 6380
Fragments arranged by size:
6380 441 201 186
BglII A'GATC_T
Cuts at: 348 680 3119 4723 6760 348
Size: 332 2439 1604 2037 796
Fragments arranged by size:
2439 2037 1604 796 332
BlpI GC'TnA_GC
Cuts at: 1526 4320 1526
Size: 2794 4414
Bme1580I G_kGCm'C
Cuts at: 885 1068 4833 4926 6050 885
Size: 183 3765 93 1124 2043
Fragments arranged by size:
3765 2043 1124 183 93
BmgBI CAC'GTC
Cuts at: 2592 3966 2592
Size: 1374 5834
BmrI ACTGGGnnnn_n'
Cuts at: 1062 1476 4420 4501 4836 6980 1062
Size: 414 2944 81 335 2144 1290
Fragments arranged by size:
2944 2144 1290 414 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1391 1857 2256 5575 1391
Size: 466 399 3319 3024
Fragments arranged by size:
3319 3024 466 399
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 252 455 664 1363 252
Size: 203 209 699 6097
Fragments arranged by size:
6097 699 209 203
Bpu10I CC'TnA_GC
Cuts at: 250 1567 1730 250
Size: 1317 163 5728
Fragments arranged by size:
5728 1317 163
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 669 1163 1834 3544 4384 5263 5977 6275 669
Size: 494 671 1710 840 879 714 298 1602
Fragments arranged by size:
1710 1602 879 840 714 671 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 1330 1421 1995 3292 3547 6912 1330
Size: 91 574 1297 255 3365 1626
Fragments arranged by size:
3365 1626 1297 574 255 91
BsaAI yAC'GTr
Cuts at: 897 4561 897
Size: 3664 3544
BsaBI GATnn'nnATC
Cuts at: 6 3176 4722 6759 6
Size: 3170 1546 2037 455
Fragments arranged by size:
3170 2037 1546 455
BsaHI Gr'CG_yC
Cuts at: 314 914 4639 4887 5589 7085 314
Size: 600 3725 248 702 1496 437
Fragments arranged by size:
3725 1496 702 600 437 248
BsaWI w'CCGG_w
Cuts at: 4397 4918 5596 6007 6154 6428 4397
Size: 521 678 411 147 274 5177
Fragments arranged by size:
5177 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2098 2128 3356 3386 2098
Size: 30 1228 30 5920
Fragments arranged by size:
5920 1228 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 633 1135 1364 1409 1421 1564 1833 2102 3418
Size: 502 229 45 12 143 269 269 1316
Cuts at: 3418 3421 633
Size: 3 4420
Fragments arranged by size:
4420 1316 502 269 269 229 143 45
12 3
BseYI C'CCAG_C
Cuts at: 260 2655 2728 4633 5391 6056 6464 260
Size: 2395 73 1905 758 665 408 1004
Fragments arranged by size:
2395 1905 1004 758 665 408 73
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2057 2529 2725 3867 4035 2057
Size: 472 196 1142 168 5230
Fragments arranged by size:
5230 1142 472 196 168
BsiEI CG_ry'CG
Cuts at: 104 4750 4796 6026 104
Size: 4646 46 1230 1286
Fragments arranged by size:
4646 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 885 1532 1608 2620 4028 4222 5000 5190 6050
Size: 647 76 1012 1408 194 778 190 860
Cuts at: 6050 885
Size: 2043
Fragments arranged by size:
2043 1408 1012 860 778 647 194 190
76
BsmI GAATG_Cn'
Cuts at: 1289 1289
Size: 7208
BsmBI CGTCTCn'nnnn_
Cuts at: 2000 2000
Size: 7208
Bsp1286I G_dGCh'C
Cuts at: 885 1068 1532 1608 2015 2620 4028 4222 4833
Size: 183 464 76 407 605 1408 194 611
Cuts at: 4833 4926 5000 5190 5252 6050 885
Size: 93 74 190 62 798 2043
Fragments arranged by size:
2043 1408 798 611 605 464 407 194
190 183 93 76 74 62
BspHI T'CATG_A
Cuts at: 5640 5640
Size: 7208
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2233 3228 4258 4472 4774 5155 2233
Size: 995 1030 214 302 381 4286
Fragments arranged by size:
4286 1030 995 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1125 5500 5554 7038 7203 50
Size: 1075 4375 54 1484 165 55
Fragments arranged by size:
4375 1484 1075 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1199 2721 3669 5120 1199
Size: 1522 948 1451 3287
Fragments arranged by size:
3287 1522 1451 948
BsrFI r'CCGG_y
Cuts at: 2790 3680 5206 5387 2790
Size: 890 1526 181 4611
Fragments arranged by size:
4611 1526 890 181
BssSI C'ACGA_G
Cuts at: 2845 3436 3584 5479 6187 2845
Size: 591 148 1895 708 3866
Fragments arranged by size:
3866 1895 708 591 148
BstAPI GCAn_nnn'nTGC
Cuts at: 391 2543 2720 3861 3881 4585 391
Size: 2152 177 1141 20 704 3014
Fragments arranged by size:
3014 2152 1141 704 177 20
BstBI TT'CG_AA
Cuts at: 5569 5569
Size: 7208
BstEII G'GTnAC_C
Cuts at: 802 802
Size: 7208
BstYI r'GATC_y
Cuts at: 1 348 680 834 3119 4233 4700 4723 5058
Size: 347 332 154 2285 1114 467 23 335
Cuts at: 5058 5304 5610 5622 5708 6760 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
2285 1114 1052 467 449 347 335 332
306 246 154 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1236 2241 1236
Size: 1005 6203
BtgI C'CryG_G
Cuts at: 107 1829 1919 3248 107
Size: 1722 90 1329 4067
Fragments arranged by size:
4067 1722 1329 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5158 5339 5502 5158
Size: 181 163 6864
Fragments arranged by size:
6864 181 163
BtsI GCAGTG_nn'
Cuts at: 201 2546 2558 2989 3708 4451 201
Size: 2345 12 431 719 743 2958
Fragments arranged by size:
2958 2345 743 719 431 12
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3684 3719 3684
Size: 35 7173
DraI TTT'AAA
Cuts at: 4207 4207
Size: 7208
DrdI GACnn_nn'nnGTC
Cuts at: 4914 6258 4914
Size: 1344 5864
EaeI y'GGCC_r
Cuts at: 306 1805 2788 4793 4967 5358 5385 6730 306
Size: 1499 983 2005 174 391 27 1345 784
Fragments arranged by size:
2005 1499 1345 983 784 391 174 27
EagI C'GGCC_G
Cuts at: 4793 4793
Size: 7208
EarI CTCTTCn'nnn_
Cuts at: 610 735 937 1277 1294 1360 1398 1553 1774
Size: 125 202 340 17 66 38 155 221
Cuts at: 1774 2967 3805 4153 5231 5441 610
Size: 1193 838 348 1078 210 2377
Fragments arranged by size:
2377 1193 1078 838 348 340 221 210
202 155 125 66 38 17
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1323 4185 4197 5416 6158 6304 7018 1323
Size: 2862 12 1219 742 146 714 1513
Fragments arranged by size:
2862 1513 1219 742 714 146 12
EcoICRI GAG'CTC
Cuts at: 4220 4220
Size: 7208
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 252 455 664 677 1363 1526 1670 1745 1916
Size: 203 209 13 686 163 144 75 171
Cuts at: 1916 1918 2075 3013 3023 4029 5033 5465 5818
Size: 2 157 938 10 1006 1004 432 353
Cuts at: 5818 6480 252
Size: 662 980
Fragments arranged by size:
1006 1004 980 938 686 662 432 353
209 203 171 163 157 144 75 13
10 2
EcoNI CCTnn'n_nnAGG
Cuts at: 280 280
Size: 7208
EcoO109I rG'GnC_Cy
Cuts at: 651 1064 1707 2158 3333 4347 651
Size: 413 643 451 1175 1014 3512
Fragments arranged by size:
3512 1175 1014 643 451 413
EcoRI G'AATT_C
Cuts at: 2857 4212 2857
Size: 1355 5853
EcoRV GAT'ATC
Cuts at: 2754 2754
Size: 7208
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 537 685 1158 2760 3945 5227 537
Size: 148 473 1602 1185 1282 2518
Fragments arranged by size:
2518 1602 1282 1185 473 148
FspI TGC'GCA
Cuts at: 4989 4989
Size: 7208
HaeII r_GCGC'y
Cuts at: 2295 3288 4642 4890 6120 6891 2295
Size: 993 1354 248 1230 771 2612
Fragments arranged by size:
2612 1354 1230 993 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 672 704 1462 1494 1775 1807 1999 2031 4051
Size: 32 758 32 281 32 192 32 2020
Cuts at: 4051 4083 5050 5080 5082 5112 672
Size: 32 967 30 2 30 2768
Fragments arranged by size:
2768 2020 967 758 281 192 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2595 3375 4247 2595
Size: 780 872 5556
Fragments arranged by size:
5556 872 780
HindIII A'AGCT_T
Cuts at: 3025 3025
Size: 7208
HpaI GTT'AAC
Cuts at: 3375 3375
Size: 7208
KasI G'GCGC_C
Cuts at: 4638 4886 4638
Size: 248 6960
KpnI G_GTAC'C
Cuts at: 4228 4228
Size: 7208
MfeI C'AATT_G
Cuts at: 3696 3696
Size: 7208
MluI A'CGCG_T
Cuts at: 6641 6641
Size: 7208
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2441 2555 5967 6151 2441
Size: 114 3412 184 3498
Fragments arranged by size:
3498 3412 184 114
MscI TGG'CCA
Cuts at: 4969 4969
Size: 7208
MslI CAynn'nnrTG
Cuts at: 166 1114 1294 1511 1535 2396 2689 2761 3091
Size: 948 180 217 24 861 293 72 330
Cuts at: 3091 3586 3959 5197 5324 166
Size: 495 373 1238 127 2050
Fragments arranged by size:
2050 1238 948 861 495 373 330 293
217 180 127 72 24
MspA1I CmG'CkG
Cuts at: 2121 2381 2396 2468 2489 2659 2705 3130 3250
Size: 260 15 72 21 170 46 425 120
Cuts at: 3250 3536 3839 3858 4049 4319 4633 4993 5777
Size: 286 303 19 191 270 314 360 784
Cuts at: 5777 6022 2121
Size: 245 3307
Fragments arranged by size:
3307 784 425 360 314 303 286 270
260 245 191 170 120 72 46 21
19 15
NaeI GCC'GGC
Cuts at: 2792 5389 2792
Size: 2597 4611
NarI GG'CG_CC
Cuts at: 4639 4887 4639
Size: 248 6960
NcoI C'CATG_G
Cuts at: 107 1829 107
Size: 1722 5486
NgoMIV G'CCGG_C
Cuts at: 2790 5387 2790
Size: 2597 4611
NsiI A_TGCA'T
Cuts at: 1887 1887
Size: 7208
NspI r_CATG'y
Cuts at: 1113 1889 3465 4261 4761 6364 1113
Size: 776 1576 796 500 1603 1957
Fragments arranged by size:
1957 1603 1576 796 776 500
PciI A'CATG_T
Cuts at: 6360 6360
Size: 7208
PflMI CCAn_nnn'nTGG
Cuts at: 736 1488 736
Size: 752 6456
PfoI T'CCnGG_A
Cuts at: 2200 2747 2200
Size: 547 6661
PmeI GTTT'AAAC
Cuts at: 4207 4207
Size: 7208
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5654 5686 5654
Size: 32 7176
PpuMI rG'GwC_Cy
Cuts at: 1707 2158 1707
Size: 451 6757
PshAI GACnn'nnGTC
Cuts at: 1441 1441
Size: 7208
PspOMI G'GGCC_C
Cuts at: 1064 1064
Size: 7208
PstI C_TGCA'G
Cuts at: 281 834 1209 1335 2230 2725 3621 4255 4940
Size: 553 375 126 895 495 896 634 685
Cuts at: 4940 281
Size: 2549
Fragments arranged by size:
2549 896 895 685 634 553 495 375
126
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7208
PvuII CAG'CTG
Cuts at: 2121 2396 2705 3536 3858 4049 4633 4993 2121
Size: 275 309 831 322 191 584 360 4336
Fragments arranged by size:
4336 831 584 360 322 309 275 191
RsrII CG'GwC_CG
Cuts at: 5403 5403
Size: 7208
SacI G_AGCT'C
Cuts at: 4222 4222
Size: 7208
SacII CC_GC'GG
Cuts at: 3251 3251
Size: 7208
SalI G'TCGA_C
Cuts at: 4245 4245
Size: 7208
SapI GCTCTTCn'nnn_
Cuts at: 937 1553 5231 5441 937
Size: 616 3678 210 2704
Fragments arranged by size:
3678 2704 616 210
SbfI CC_TGCA'GG
Cuts at: 281 3621 4255 281
Size: 3340 634 3234
Fragments arranged by size:
3340 3234 634
ScaI AGT'ACT
Cuts at: 1371 6725 1371
Size: 5354 1854
SfcI C'TryA_G
Cuts at: 33 277 830 1205 1331 1712 2226 2721 2778
Size: 244 553 375 126 381 514 495 57
Cuts at: 2778 3470 3617 4251 4936 5904 6095 33
Size: 692 147 634 685 968 191 1146
Fragments arranged by size:
1146 968 692 685 634 553 514 495
381 375 244 191 147 126 57
SfoI GGC'GCC
Cuts at: 4640 4888 4640
Size: 248 6960
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7208
SmaI CCC'GGG
Cuts at: 361 2003 4230 361
Size: 1642 2227 3339
Fragments arranged by size:
3339 2227 1642
SmlI C'TyrA_G
Cuts at: 684 1178 1849 3559 4363 5278 5992 6254 684
Size: 494 671 1710 804 915 714 262 1638
Fragments arranged by size:
1710 1638 915 804 714 671 494 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7208
SphI G_CATG'C
Cuts at: 4261 4761 4261
Size: 500 6708
SspI AAT'ATT
Cuts at: 2829 2829
Size: 7208
StuI AGG'CCT
Cuts at: 3892 3892
Size: 7208
StyI C'CwwG_G
Cuts at: 107 808 1187 1829 2190 2431 3887 3915 3949
Size: 701 379 642 361 241 1456 28 34
Cuts at: 3949 4342 4696 107
Size: 393 354 2619
Fragments arranged by size:
2619 1456 701 642 393 379 361 354
241 34 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1026 1547 1755 2751 3466 4092 4791 4924 5383
Size: 521 208 996 715 626 699 133 459
Cuts at: 5383 5592 1026
Size: 209 2642
Fragments arranged by size:
2642 996 715 699 626 521 459 209
208 133
TatI w'GTAC_w
Cuts at: 1369 6723 1369
Size: 5354 1854
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 170 202 2098 2130 2504 2536 6725 6757 170
Size: 32 1896 32 374 32 4189 32 621
Fragments arranged by size:
4189 1896 621 374 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 5005 5005
Size: 7208
XbaI T'CTAG_A
Cuts at: 4239 4239
Size: 7208
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 926 1626 4703 926
Size: 700 3077 3431
Fragments arranged by size:
3431 3077 700
XmaI C'CCGG_G
Cuts at: 359 2001 4228 359
Size: 1642 2227 3339
Fragments arranged by size:
3339 2227 1642
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AgeI AloI AscI AvrII BmtI BsiWI
BspEI BsrGI BssHII BstXI BstZ17I ClaI DraIII FseI
FspAI NdeI NheI NotI NruI PacI PasI PmlI
PsiI PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI
SrfI SwaI XhoI XmnI ZraI