Restriction map for
pF1KA1091
(Circular) (Six-base) MAPSORT of: pF1KA1091.seq Check: 7926 from: 1 to: 6974
pF1KA1091 6974 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2240 2240
Size: 6974
AatII G_ACGT'C
Cuts at: 3946 3946
Size: 6974
AccI GT'mk_AC
Cuts at: 4012 4012
Size: 6974
Acc65I G'GTAC_C
Cuts at: 3990 3990
Size: 6974
AclI AA'CG_TT
Cuts at: 6814 6814
Size: 6974
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 305 1394 1432 2392 2830 4799 5231 5584 6246
Size: 1089 38 960 438 1969 432 353 662
Cuts at: 6246 305
Size: 1033
Fragments arranged by size:
1969 1089 1033 960 662 438 432 353
38
AfeI AGC'GCT
Cuts at: 676 2857 676
Size: 2181 4793
AflII C'TTAA_G
Cuts at: 1495 1495
Size: 6974
AflIII A'CryG_T
Cuts at: 3845 4326 6126 6407 3845
Size: 481 1800 281 4412
Fragments arranged by size:
4412 1800 481 281
AgeI A'CCGG_T
Cuts at: 1217 1217
Size: 6974
AhdI GACnn_n'nnGTC
Cuts at: 210 210
Size: 6974
AleI CACnn'nnGTG
Cuts at: 658 2464 658
Size: 1806 5168
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 571 571
Size: 6974
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3542 3574 3542
Size: 32 6942
AlwNI CAG_nnn'CTG
Cuts at: 1068 3768 4279 5717 1068
Size: 2700 511 1438 2325
Fragments arranged by size:
2700 2325 1438 511
ApaLI G'TGCA_C
Cuts at: 5812 5812
Size: 6974
ApoI r'AATT_y
Cuts at: 294 1364 3978 6327 6583 294
Size: 1070 2614 2349 256 685
Fragments arranged by size:
2614 2349 1070 685 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6974
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6974
AvaI C'yCGr_G
Cuts at: 985 1925 3994 985
Size: 940 2069 3965
Fragments arranged by size:
3965 2069 940
BamHI G'GATC_C
Cuts at: 3284 3999 3284
Size: 715 6259
BanI G'GyrC_C
Cuts at: 138 256 858 3990 4404 4652 4687 138
Size: 118 602 3132 414 248 35 2425
Fragments arranged by size:
3132 2425 602 414 248 118 35
BanII G_rGCy'C
Cuts at: 136 452 1808 2837 3013 3693 3988 5018 136
Size: 316 1356 1029 176 680 295 1030 2092
Fragments arranged by size:
2092 1356 1030 1029 680 316 295 176
BbeI G_GCGC'C
Cuts at: 142 4408 4656 142
Size: 4266 248 2460
Fragments arranged by size:
4266 2460 248
BbsI GAAGACnn'nnnn_
Cuts at: 656 1583 2943 3591 656
Size: 927 1360 648 4039
Fragments arranged by size:
4039 1360 927 648
BbvCI CC'TCA_GC
Cuts at: 806 806
Size: 6974
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3948 3982 3948
Size: 34 6940
BciVI GTATCCnnnnn_n'
Cuts at: 4872 5928 6974 4872
Size: 1056 1046 4872
Fragments arranged by size:
4872 1056 1046
BclI T'GATC_A
Cuts at: 3866 4494 3866
Size: 628 6346
BglI GCCn_nnn'nGGC
Cuts at: 414 3820 414
Size: 3406 3568
BglII A'GATC_T
Cuts at: 542 4489 6526 542
Size: 3947 2037 990
Fragments arranged by size:
3947 2037 990
BlpI GC'TnA_GC
Cuts at: 4086 4086
Size: 6974
Bme1580I G_kGCm'C
Cuts at: 1023 4599 4692 5816 1023
Size: 3576 93 1124 2181
Fragments arranged by size:
3576 2181 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 137 1553 2156 2184 3359 3431 4186 4267 4602
Size: 1416 603 28 1175 72 755 81 335
Cuts at: 4602 6746 137
Size: 2144 365
Fragments arranged by size:
2144 1416 1175 755 603 365 335 81
72 28
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 504 1419 1455 3811 5341 504
Size: 915 36 2356 1530 2137
Fragments arranged by size:
2356 2137 1530 915 36
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 1048 1249 1855 2538 3298 3958 217
Size: 831 201 606 683 760 660 3233
Fragments arranged by size:
3233 831 760 683 660 606 201
Bpu10I CC'TnA_GC
Cuts at: 806 1802 2120 806
Size: 996 318 5660
Fragments arranged by size:
5660 996 318
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 847 1223 1809 4150 5029 5743 6041 847
Size: 376 586 2341 879 714 298 1780
Fragments arranged by size:
2341 1780 879 714 586 376 298
BsaI GGTCTCn'nnnn_
Cuts at: 181 617 918 1831 6678 181
Size: 436 301 913 4847 477
Fragments arranged by size:
4847 913 477 436 301
BsaAI yAC'GTr
Cuts at: 4327 4327
Size: 6974
BsaBI GATnn'nnATC
Cuts at: 6 3573 4488 6525 6
Size: 3567 915 2037 455
Fragments arranged by size:
3567 2037 915 455
BsaHI Gr'CG_yC
Cuts at: 139 3943 4405 4653 5355 6851 139
Size: 3804 462 248 702 1496 262
Fragments arranged by size:
3804 1496 702 462 262 248
BsaWI w'CCGG_w
Cuts at: 1217 3374 4163 4684 5362 5773 5920 6194 1217
Size: 2157 789 521 678 411 147 274 1997
Fragments arranged by size:
2157 1997 789 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 985 1015 1952 1982 985
Size: 30 937 30 5977
Fragments arranged by size:
5977 937 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2809 2809
Size: 6974
BseYI C'CCAG_C
Cuts at: 1143 1582 1720 3003 3311 4399 5157 5822 6230
Size: 439 138 1283 308 1088 758 665 408
Cuts at: 6230 1143
Size: 1887
Fragments arranged by size:
1887 1283 1088 758 665 439 408 308
138
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 3824 3824
Size: 6974
BsiEI CG_ry'CG
Cuts at: 104 4516 4562 5792 104
Size: 4412 46 1230 1286
Fragments arranged by size:
4412 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2837 3988 4766 4956 5816 2837
Size: 1151 778 190 860 3995
Fragments arranged by size:
3995 1151 860 778 190
BsmI GAATG_Cn'
Cuts at: 401 421 1197 1479 2184 2383 401
Size: 20 776 282 705 199 4992
Fragments arranged by size:
4992 776 705 282 199 20
BsmBI CGTCTCn'nnnn_
Cuts at: 720 1078 3934 720
Size: 358 2856 3760
Fragments arranged by size:
3760 2856 358
Bsp1286I G_dGCh'C
Cuts at: 136 452 1023 1808 2837 3013 3693 3988 4599
Size: 316 571 785 1029 176 680 295 611
Cuts at: 4599 4692 4766 4956 5018 5816 136
Size: 93 74 190 62 798 1294
Fragments arranged by size:
1294 1029 798 785 680 611 571 316
295 190 176 93 74 62
BspEI T'CCGG_A
Cuts at: 3374 3374
Size: 6974
BspHI T'CATG_A
Cuts at: 752 1881 5406 752
Size: 1129 3525 2320
Fragments arranged by size:
3525 2320 1129
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1820 2240 4024 4238 4540 4921 1820
Size: 420 1784 214 302 381 3873
Fragments arranged by size:
3873 1784 420 381 302 214
BsrBI CCG'CTC
Cuts at: 50 864 3282 5266 5320 6804 6969 50
Size: 814 2418 1984 54 1484 165 55
Fragments arranged by size:
2418 1984 1484 814 165 55 54
BsrDI GCAATG_nn'
Cuts at: 560 1386 1460 3913 4886 560
Size: 826 74 2453 973 2648
Fragments arranged by size:
2648 2453 973 826 74
BsrFI r'CCGG_y
Cuts at: 878 1217 2588 3242 4972 5153 878
Size: 339 1371 654 1730 181 2699
Fragments arranged by size:
2699 1730 1371 654 339 181
BssSI C'ACGA_G
Cuts at: 5245 5953 5245
Size: 708 6266
BstAPI GCAn_nnn'nTGC
Cuts at: 3915 4351 3915
Size: 436 6538
BstBI TT'CG_AA
Cuts at: 5335 5335
Size: 6974
BstXI CCAn_nnnn'nTGG
Cuts at: 1209 1320 2283 3180 1209
Size: 111 963 897 5003
Fragments arranged by size:
5003 963 897 111
BstYI r'GATC_y
Cuts at: 1 542 1596 1652 2513 2690 3035 3284 3428
Size: 541 1054 56 861 177 345 249 144
Cuts at: 3428 3999 4466 4489 4824 5070 5376 5388 5474
Size: 571 467 23 335 246 306 12 86
Cuts at: 5474 6526 1
Size: 1052 449
Fragments arranged by size:
1054 1052 861 571 541 467 449 345
335 306 249 246 177 144 86 56
23 12
Bsu36I CC'TnA_GG
Cuts at: 2625 2686 3317 3668 2625
Size: 61 631 351 5931
Fragments arranged by size:
5931 631 351 61
BtgI C'CryG_G
Cuts at: 2780 3031 2780
Size: 251 6723
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4924 5105 5268 4924
Size: 181 163 6630
Fragments arranged by size:
6630 181 163
BtsI GCAGTG_nn'
Cuts at: 555 817 1398 3020 3713 3836 4217 555
Size: 262 581 1622 693 123 381 3312
Fragments arranged by size:
3312 1622 693 581 381 262 123
DraI TTT'AAA
Cuts at: 3573 3973 3573
Size: 400 6574
DrdI GACnn_nn'nnGTC
Cuts at: 163 961 4680 6024 163
Size: 798 3719 1344 1113
Fragments arranged by size:
3719 1344 1113 798
EaeI y'GGCC_r
Cuts at: 876 3147 3240 4559 4733 5124 5151 6496 876
Size: 2271 93 1319 174 391 27 1345 1354
Fragments arranged by size:
2271 1354 1345 1319 391 174 93 27
EagI C'GGCC_G
Cuts at: 4559 4559
Size: 6974
EarI CTCTTCn'nnn_
Cuts at: 518 1408 2465 3592 4997 5207 518
Size: 890 1057 1127 1405 210 2285
Fragments arranged by size:
2285 1405 1127 1057 890 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 140 1096 2214 2287 5182 5924 6070 6784 140
Size: 956 1118 73 2895 742 146 714 330
Fragments arranged by size:
2895 1118 956 742 714 330 146 73
EcoICRI GAG'CTC
Cuts at: 2835 3986 2835
Size: 1151 5823
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 305 1048 1249 1394 1432 1855 2392 2538
Size: 88 743 201 145 38 423 537 146
Cuts at: 2538 2830 3298 3958 4799 5231 5584 6246 217
Size: 292 468 660 841 432 353 662 945
Fragments arranged by size:
945 841 743 662 660 537 468 432
423 353 292 201 146 145 88 38
EcoNI CCTnn'n_nnAGG
Cuts at: 2371 2701 3952 2371
Size: 330 1251 5393
Fragments arranged by size:
5393 1251 330
EcoO109I rG'GnC_Cy
Cuts at: 336 2364 4113 336
Size: 2028 1749 3197
Fragments arranged by size:
3197 2028 1749
EcoRI G'AATT_C
Cuts at: 1364 3978 1364
Size: 2614 4360
EcoRV GAT'ATC
Cuts at: 349 349
Size: 6974
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1399 4993 1399
Size: 3594 3380
FspI TGC'GCA
Cuts at: 4755 4755
Size: 6974
HaeII r_GCGC'y
Cuts at: 142 678 2859 4408 4656 5886 6657 142
Size: 536 2181 1549 248 1230 771 459
Fragments arranged by size:
2181 1549 1230 771 536 459 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 196 228 1080 1112 1204 1236 1720 1752 1765
Size: 32 852 32 92 32 484 32 13
Cuts at: 1765 1797 1950 1982 2505 2537 2653 2685 4816
Size: 32 153 32 523 32 116 32 2131
Cuts at: 4816 4846 4848 4878 196
Size: 30 2 30 2292
Fragments arranged by size:
2292 2131 852 523 484 153 116 92
32 32 32 32 32 32 32 32
30 30 13 2
HincII GTy'rAC
Cuts at: 1932 4013 1932
Size: 2081 4893
HindIII A'AGCT_T
Cuts at: 3127 3127
Size: 6974
KasI G'GCGC_C
Cuts at: 138 4404 4652 138
Size: 4266 248 2460
Fragments arranged by size:
4266 2460 248
KpnI G_GTAC'C
Cuts at: 3994 3994
Size: 6974
MfeI C'AATT_G
Cuts at: 2021 2346 2021
Size: 325 6649
MluI A'CGCG_T
Cuts at: 6407 6407
Size: 6974
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 745 1544 1740 2339 5733 5917 745
Size: 799 196 599 3394 184 1802
Fragments arranged by size:
3394 1802 799 599 196 184
MscI TGG'CCA
Cuts at: 3149 4735 3149
Size: 1586 5388
MslI CAynn'nnrTG
Cuts at: 658 742 1127 1202 1391 1684 1886 2464 3848
Size: 84 385 75 189 293 202 578 1384
Cuts at: 3848 4963 5090 658
Size: 1115 127 2542
Fragments arranged by size:
2542 1384 1115 578 385 293 202 189
127 84 75
MspA1I CmG'CkG
Cuts at: 3351 4085 4399 4759 5543 5788 3351
Size: 734 314 360 784 245 4537
Fragments arranged by size:
4537 784 734 360 314 245
NaeI GCC'GGC
Cuts at: 5155 5155
Size: 6974
NarI GG'CG_CC
Cuts at: 139 4405 4653 139
Size: 4266 248 2460
Fragments arranged by size:
4266 2460 248
NcoI C'CATG_G
Cuts at: 2780 3031 2780
Size: 251 6723
NgoMIV G'CCGG_C
Cuts at: 5153 5153
Size: 6974
NspI r_CATG'y
Cuts at: 576 604 764 1126 3849 4027 4527 6130 576
Size: 28 160 362 2723 178 500 1603 1420
Fragments arranged by size:
2723 1603 1420 500 362 178 160 28
PasI CC'CwG_GG
Cuts at: 443 443
Size: 6974
PciI A'CATG_T
Cuts at: 3845 6126 3845
Size: 2281 4693
PflMI CCAn_nnn'nTGG
Cuts at: 874 2765 3032 3120 874
Size: 1891 267 88 4728
Fragments arranged by size:
4728 1891 267 88
PfoI T'CCnGG_A
Cuts at: 1331 1655 1832 2267 2504 3431 3614 1331
Size: 324 177 435 237 927 183 4691
Fragments arranged by size:
4691 927 435 324 237 183 177
PmeI GTTT'AAAC
Cuts at: 3973 3973
Size: 6974
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5420 5452 5420
Size: 32 6942
PpuMI rG'GwC_Cy
Cuts at: 336 336
Size: 6974
PstI C_TGCA'G
Cuts at: 550 674 779 2129 3356 3911 4021 4706 550
Size: 124 105 1350 1227 555 110 685 2818
Fragments arranged by size:
2818 1350 1227 685 555 124 110 105
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6974
PvuII CAG'CTG
Cuts at: 4399 4759 4399
Size: 360 6614
RsrII CG'GwC_CG
Cuts at: 5169 5169
Size: 6974
SacI G_AGCT'C
Cuts at: 2837 3988 2837
Size: 1151 5823
SalI G'TCGA_C
Cuts at: 4011 4011
Size: 6974
SapI GCTCTTCn'nnn_
Cuts at: 3592 4997 5207 3592
Size: 1405 210 5359
Fragments arranged by size:
5359 1405 210
SbfI CC_TGCA'GG
Cuts at: 4021 4021
Size: 6974
ScaI AGT'ACT
Cuts at: 6491 6491
Size: 6974
SfcI C'TryA_G
Cuts at: 33 546 670 775 2125 3352 3907 4017 4702
Size: 513 124 105 1350 1227 555 110 685
Cuts at: 4702 5670 5861 33
Size: 968 191 1146
Fragments arranged by size:
1350 1227 1146 968 685 555 513 191
124 110 105
SfoI GGC'GCC
Cuts at: 140 4406 4654 140
Size: 4266 248 2460
Fragments arranged by size:
4266 2460 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6974
SmaI CCC'GGG
Cuts at: 3996 3996
Size: 6974
SmlI C'TyrA_G
Cuts at: 826 1238 1495 1824 4129 5044 5758 6020 826
Size: 412 257 329 2305 915 714 262 1780
Fragments arranged by size:
2305 1780 915 714 412 329 262 257
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6974
SphI G_CATG'C
Cuts at: 4027 4527 4027
Size: 500 6474
StyI C'CwwG_G
Cuts at: 1529 2780 3031 3545 4108 4462 1529
Size: 1251 251 514 563 354 4041
Fragments arranged by size:
4041 1251 563 514 354 251
SwaI ATTT'AAAT
Cuts at: 3573 3573
Size: 6974
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 756 2078 2757 3046 3411 4557 4690 5149 5358
Size: 1322 679 289 365 1146 133 459 209
Cuts at: 5358 756
Size: 2372
Fragments arranged by size:
2372 1322 1146 679 459 365 289 209
133
TatI w'GTAC_w
Cuts at: 227 1905 2136 2261 6489 227
Size: 1678 231 125 4228 712
Fragments arranged by size:
4228 1678 712 231 125
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1952 1984 2519 2551 6491 6523 1952
Size: 32 535 32 3940 32 2403
Fragments arranged by size:
3940 2403 535 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4771 4771
Size: 6974
XbaI T'CTAG_A
Cuts at: 4005 4005
Size: 6974
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2400 3157 3180 4469 2400
Size: 757 23 1289 4905
Fragments arranged by size:
4905 1289 757 23
XmaI C'CCGG_G
Cuts at: 3994 3994
Size: 6974
XmnI GAAnn'nnTTC
Cuts at: 3472 3472
Size: 6974
ZraI GAC'GTC
Cuts at: 3944 3944
Size: 6974
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
ApaI AscI AvrII BaeI BfrBI BmgBI BmtI BsiWI
BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII FseI
FspAI HpaI NdeI NheI NotI NruI NsiI PacI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI XhoI