Restriction map for
pF1KA1097
(Circular) (Six-base) MAPSORT of: pF1KA1097.seq Check: 9002 from: 1 to: 5846
pF1KA1097 5846 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AccI GT'mk_AC
Cuts at: 1190 1276 2884 1190
Size: 86 1608 4152
Fragments arranged by size:
4152 1608 86
Acc65I G'GTAC_C
Cuts at: 2862 2862
Size: 5846
AclI AA'CG_TT
Cuts at: 5686 5686
Size: 5846
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1055 1166 1223 1924 2120 2212 2729 2774 3671
Size: 111 57 701 196 92 517 45 897
Cuts at: 3671 4103 4850 5118 1055
Size: 432 747 268 1783
Fragments arranged by size:
1783 897 747 701 517 432 268 196
111 92 57 45
AflIII A'CryG_T
Cuts at: 3198 4302 5279 3198
Size: 1104 977 3765
Fragments arranged by size:
3765 1104 977
AgeI A'CCGG_T
Cuts at: 4234 4234
Size: 5846
AhdI GACnn_n'nnGTC
Cuts at: 1497 2422 1497
Size: 925 4921
AleI CACnn'nnGTG
Cuts at: 1635 1635
Size: 5846
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2584 2584
Size: 5846
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2207 2239 2207
Size: 32 5814
AlwNI CAG_nnn'CTG
Cuts at: 754 1482 2695 3151 4718 754
Size: 728 1213 456 1567 1882
Fragments arranged by size:
1882 1567 1213 728 456
ApaLI G'TGCA_C
Cuts at: 4616 4616
Size: 5846
ApoI r'AATT_y
Cuts at: 835 1140 1153 2077 2196 2260 2501 2721 2850
Size: 305 13 924 119 64 241 220 129
Cuts at: 2850 5199 5455 835
Size: 2349 256 1226
Fragments arranged by size:
2349 1226 924 305 256 241 220 129
119 64 13
AseI AT'TA_AT
Cuts at: 20 1130 20
Size: 1110 4736
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5846
AvaI C'yCGr_G
Cuts at: 1510 2866 1510
Size: 1356 4490
BamHI G'GATC_C
Cuts at: 2871 2871
Size: 5846
BanI G'GyrC_C
Cuts at: 1365 2862 3276 3524 3559 1365
Size: 1497 414 248 35 3652
Fragments arranged by size:
3652 1497 414 248 35
BanII G_rGCy'C
Cuts at: 2860 3890 2860
Size: 1030 4816
BbeI G_GCGC'C
Cuts at: 3280 3528 3280
Size: 248 5598
BbsI GAAGACnn'nnnn_
Cuts at: 947 1559 2360 947
Size: 612 801 4433
Fragments arranged by size:
4433 801 612
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1606 1640 1606
Size: 34 5812
BciVI GTATCCnnnnn_n'
Cuts at: 3744 4505 5846 3744
Size: 761 1341 3744
Fragments arranged by size:
3744 1341 761
BclI T'GATC_A
Cuts at: 2085 3366 2085
Size: 1281 4565
BglII A'GATC_T
Cuts at: 1524 3361 5398 1524
Size: 1837 2037 1972
Fragments arranged by size:
2037 1972 1837
BlpI GC'TnA_GC
Cuts at: 1562 2958 1562
Size: 1396 4450
Bme1580I G_kGCm'C
Cuts at: 3471 3564 4620 3471
Size: 93 1056 4697
Fragments arranged by size:
4697 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 467 3058 3139 3474 5618 467
Size: 2591 81 335 2144 695
Fragments arranged by size:
2591 2144 695 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4213 4213
Size: 5846
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 2528 2606 259
Size: 2269 78 3499
Fragments arranged by size:
3499 2269 78
Bpu10I CC'TnA_GC
Cuts at: 1955 2677 1955
Size: 722 5124
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 815 3022 3901 4393 4691 815
Size: 2207 879 492 298 1970
Fragments arranged by size:
2207 1970 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1507 5550 1507
Size: 4043 1803
BsaAI yAC'GTr
Cuts at: 3199 3199
Size: 5846
BsaBI GATnn'nnATC
Cuts at: 6 2615 3360 5397 6
Size: 2609 745 2037 455
Fragments arranged by size:
2609 2037 745 455
BsaHI Gr'CG_yC
Cuts at: 3277 3525 4227 5723 3277
Size: 248 702 1496 3400
Fragments arranged by size:
3400 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2772 3035 3556 4234 4508 4655 5066 2772
Size: 263 521 678 274 147 411 3552
Fragments arranged by size:
3552 678 521 411 274 263 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2207 2237 2207
Size: 30 5816
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 485 940 2196 485
Size: 455 1256 4135
Fragments arranged by size:
4135 1256 455
BseYI C'CCAG_C
Cuts at: 3271 4029 4606 5102 3271
Size: 758 577 496 4015
Fragments arranged by size:
4015 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1495 1495
Size: 5846
BsiEI CG_ry'CG
Cuts at: 104 3388 3434 4642 104
Size: 3284 46 1208 1308
Fragments arranged by size:
3284 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2860 3638 3828 4620 2860
Size: 778 190 792 4086
Fragments arranged by size:
4086 792 778 190
BsmI GAATG_Cn'
Cuts at: 609 1569 609
Size: 960 4886
Bsp1286I G_dGCh'C
Cuts at: 2860 3471 3564 3638 3828 3890 4620 2860
Size: 611 93 74 190 62 730 4086
Fragments arranged by size:
4086 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 5066 5066
Size: 5846
BspHI T'CATG_A
Cuts at: 864 5022 864
Size: 4158 1688
BspMI ACCTGCnnnn'nnnn_
Cuts at: 699 2393 2896 3110 3412 3793 699
Size: 1694 503 214 302 381 2752
Fragments arranged by size:
2752 1694 503 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4138 4192 5676 5841 50
Size: 4088 54 1484 165 55
Fragments arranged by size:
4088 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 409 3758 409
Size: 3349 2497
BsrFI r'CCGG_y
Cuts at: 3844 4025 4234 3844
Size: 181 209 5456
Fragments arranged by size:
5456 209 181
BsrGI T'GTAC_A
Cuts at: 1775 2118 2435 1775
Size: 343 317 5186
Fragments arranged by size:
5186 343 317
BssSI C'ACGA_G
Cuts at: 4117 4475 4117
Size: 358 5488
BstAPI GCAn_nnn'nTGC
Cuts at: 3223 3223
Size: 5846
BstBI TT'CG_AA
Cuts at: 120 1264 4207 120
Size: 1144 2943 1759
Fragments arranged by size:
2943 1759 1144
BstXI CCAn_nnnn'nTGG
Cuts at: 227 1637 227
Size: 1410 4436
BstYI r'GATC_y
Cuts at: 1 751 903 1286 1524 1935 2616 2871 3338
Size: 750 152 383 238 411 681 255 467
Cuts at: 3338 3361 3696 3942 4954 5040 5052 5398 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 750 681 467 449 411 383 346
335 255 246 238 152 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3796 3977 4140 3796
Size: 181 163 5502
Fragments arranged by size:
5502 181 163
BtsI GCAGTG_nn'
Cuts at: 1483 2578 3089 1483
Size: 1095 511 4240
Fragments arranged by size:
4240 1095 511
DraI TTT'AAA
Cuts at: 465 2845 465
Size: 2380 3466
DrdI GACnn_nn'nnGTC
Cuts at: 3552 4410 3552
Size: 858 4988
EaeI y'GGCC_r
Cuts at: 1354 2697 3431 3605 3996 4023 5368 1354
Size: 1343 734 174 391 27 1345 1832
Fragments arranged by size:
1832 1345 1343 734 391 174 27
EagI C'GGCC_G
Cuts at: 3431 3431
Size: 5846
EarI CTCTTCn'nnn_
Cuts at: 347 1660 2149 2159 3869 4079 347
Size: 1313 489 10 1710 210 2114
Fragments arranged by size:
2114 1710 1313 489 210 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2479 4054 4364 4510 5656 2479
Size: 1575 310 146 1146 2669
Fragments arranged by size:
2669 1575 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2858 2858
Size: 5846
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 1055 1166 1223 1924 2120 2212 2528 2606
Size: 796 111 57 701 196 92 316 78
Cuts at: 2606 2729 2774 3671 4103 4850 5118 259
Size: 123 45 897 432 747 268 987
Fragments arranged by size:
987 897 796 747 701 432 316 268
196 123 111 92 78 57 45
EcoNI CCTnn'n_nnAGG
Cuts at: 1340 1340
Size: 5846
EcoO109I rG'GnC_Cy
Cuts at: 2626 2749 2985 2626
Size: 123 236 5487
Fragments arranged by size:
5487 236 123
EcoRI G'AATT_C
Cuts at: 835 2850 835
Size: 2015 3831
EcoRV GAT'ATC
Cuts at: 2192 2192
Size: 5846
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 565 856 1747 3865 565
Size: 291 891 2118 2546
Fragments arranged by size:
2546 2118 891 291
FspI TGC'GCA
Cuts at: 3627 3627
Size: 5846
HaeII r_GCGC'y
Cuts at: 3280 3528 4550 5529 3280
Size: 248 1022 979 3597
Fragments arranged by size:
3597 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 955 987 1156 1162 1188 1194 1483 1515 1971
Size: 32 169 6 26 6 289 32 456
Cuts at: 1971 1978 2003 2010 2358 2390 2408 2440 3688
Size: 7 25 7 348 32 18 32 1248
Cuts at: 3688 3718 3720 3750 955
Size: 30 2 30 3051
Fragments arranged by size:
3051 1248 456 348 289 169 32 32
32 32 30 30 26 25 18 7
7 6 6 2
HincII GTy'rAC
Cuts at: 1191 2427 2885 1191
Size: 1236 458 4152
Fragments arranged by size:
4152 1236 458
HindIII A'AGCT_T
Cuts at: 2132 2132
Size: 5846
KasI G'GCGC_C
Cuts at: 3276 3524 3276
Size: 248 5598
KpnI G_GTAC'C
Cuts at: 2866 2866
Size: 5846
MluI A'CGCG_T
Cuts at: 5279 5279
Size: 5846
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 823 935 1609 4517 4701 823
Size: 112 674 2908 184 1968
Fragments arranged by size:
2908 1968 674 184 112
MscI TGG'CCA
Cuts at: 1356 2699 3607 1356
Size: 1343 908 3595
Fragments arranged by size:
3595 1343 908
MslI CAynn'nnrTG
Cuts at: 1265 1635 2321 2566 3835 3962 1265
Size: 370 686 245 1269 127 3149
Fragments arranged by size:
3149 1269 686 370 245 127
MspA1I CmG'CkG
Cuts at: 2422 2957 3271 3631 4644 4889 2422
Size: 535 314 360 1013 245 3379
Fragments arranged by size:
3379 1013 535 360 314 245
NaeI GCC'GGC
Cuts at: 4027 4027
Size: 5846
NarI GG'CG_CC
Cuts at: 3277 3525 3277
Size: 248 5598
NdeI CA'TA_TG
Cuts at: 255 1623 1983 255
Size: 1368 360 4118
Fragments arranged by size:
4118 1368 360
NgoMIV G'CCGG_C
Cuts at: 4025 4025
Size: 5846
NspI r_CATG'y
Cuts at: 236 2899 3399 4306 236
Size: 2663 500 907 1776
Fragments arranged by size:
2663 1776 907 500
PciI A'CATG_T
Cuts at: 4302 4302
Size: 5846
PflMI CCAn_nnn'nTGG
Cuts at: 1543 1543
Size: 5846
PfoI T'CCnGG_A
Cuts at: 455 455
Size: 5846
PmeI GTTT'AAAC
Cuts at: 2845 2845
Size: 5846
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2207 2239 4985 5017 2207
Size: 32 2746 32 3036
Fragments arranged by size:
3036 2746 32 32
PpuMI rG'GwC_Cy
Cuts at: 2626 2626
Size: 5846
PstI C_TGCA'G
Cuts at: 2732 2893 3578 2732
Size: 161 685 5000
Fragments arranged by size:
5000 685 161
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5846
PvuII CAG'CTG
Cuts at: 2422 3271 3631 2422
Size: 849 360 4637
Fragments arranged by size:
4637 849 360
RsrII CG'GwC_CG
Cuts at: 4041 4041
Size: 5846
SacI G_AGCT'C
Cuts at: 2860 2860
Size: 5846
SalI G'TCGA_C
Cuts at: 1189 2883 1189
Size: 1694 4152
SapI GCTCTTCn'nnn_
Cuts at: 2149 3869 4079 2149
Size: 1720 210 3916
Fragments arranged by size:
3916 1720 210
SbfI CC_TGCA'GG
Cuts at: 2893 2893
Size: 5846
ScaI AGT'ACT
Cuts at: 5363 5363
Size: 5846
SexAI A'CCwGG_T
Cuts at: 2357 2357
Size: 5846
SfcI C'TryA_G
Cuts at: 33 1592 2148 2728 2889 3574 4567 4758 33
Size: 1559 556 580 161 685 993 191 1121
Fragments arranged by size:
1559 1121 993 685 580 556 191 161
SfoI GGC'GCC
Cuts at: 3278 3526 3278
Size: 248 5598
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5846
SmaI CCC'GGG
Cuts at: 2868 2868
Size: 5846
SmlI C'TyrA_G
Cuts at: 830 1510 3001 3916 4408 4670 830
Size: 680 1491 915 492 262 2006
Fragments arranged by size:
2006 1491 915 680 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5846
SphI G_CATG'C
Cuts at: 2899 3399 2899
Size: 500 5346
SspI AAT'ATT
Cuts at: 582 582
Size: 5846
StyI C'CwwG_G
Cuts at: 186 213 2980 3334 186
Size: 27 2767 354 2698
Fragments arranged by size:
2767 2698 354 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2822 3429 3562 4021 4230 2822
Size: 607 133 459 209 4438
Fragments arranged by size:
4438 607 459 209 133
TatI w'GTAC_w
Cuts at: 1775 1827 2118 2435 5361 1775
Size: 52 291 317 2926 2260
Fragments arranged by size:
2926 2260 317 291 52
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 743 775 1271 1303 5363 5395 743
Size: 32 496 32 4060 32 1194
Fragments arranged by size:
4060 1194 496 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3643 3643
Size: 5846
XbaI T'CTAG_A
Cuts at: 1021 2877 1021
Size: 1856 3990
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3341 3341
Size: 5846
XhoI C'TCGA_G
Cuts at: 1510 1510
Size: 5846
XmaI C'CCGG_G
Cuts at: 2866 2866
Size: 5846
XmnI GAAnn'nnTTC
Cuts at: 839 839
Size: 5846
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII ApaI AscI AvrII BaeI
BbvCI BfrBI BglI BmgBI BmtI BsiWI BsmBI BssHII
BstEII BstZ17I Bsu36I BtgI ClaI CspCI DraIII FseI
FspAI HpaI MfeI NcoI NheI NotI NruI NsiI
PacI PasI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI StuI SwaI
ZraI