Restriction map for
pF1KA1101
(Circular) (Six-base) MAPSORT of: pF1KA1101.seq Check: 6092 from: 1 to: 4694
pF1KA1101 4694 bp
With 182 enzymes: SgfI *
September 18, 2009 16:59 ..
AccI GT'mk_AC
Cuts at: 1529 1732 1529
Size: 203 4491
Acc65I G'GTAC_C
Cuts at: 1710 1710
Size: 4694
AclI AA'CG_TT
Cuts at: 4534 4534
Size: 4694
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 713 1358 2519 2951 3698 3966 713
Size: 645 1161 432 747 268 1441
Fragments arranged by size:
1441 1161 747 645 432 268
AflIII A'CryG_T
Cuts at: 2046 3150 4127 2046
Size: 1104 977 2613
Fragments arranged by size:
2613 1104 977
AgeI A'CCGG_T
Cuts at: 3082 3082
Size: 4694
AleI CACnn'nnGTG
Cuts at: 449 449
Size: 4694
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 389 421 389
Size: 32 4662
AlwNI CAG_nnn'CTG
Cuts at: 1999 3566 1999
Size: 1567 3127
ApaLI G'TGCA_C
Cuts at: 3464 3464
Size: 4694
ApoI r'AATT_y
Cuts at: 296 973 1000 1132 1209 1431 1465 1698 4047
Size: 677 27 132 77 222 34 233 2349
Cuts at: 4047 4303 296
Size: 256 687
Fragments arranged by size:
2349 687 677 256 233 222 132 77
34 27
AseI AT'TA_AT
Cuts at: 20 20
Size: 4694
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4694
AvaI C'yCGr_G
Cuts at: 120 1187 1714 120
Size: 1067 527 3100
Fragments arranged by size:
3100 1067 527
BamHI G'GATC_C
Cuts at: 1719 1719
Size: 4694
BanI G'GyrC_C
Cuts at: 670 687 1710 2124 2372 2407 670
Size: 17 1023 414 248 35 2957
Fragments arranged by size:
2957 1023 414 248 35 17
BanII G_rGCy'C
Cuts at: 1220 1708 2738 1220
Size: 488 1030 3176
Fragments arranged by size:
3176 1030 488
BbeI G_GCGC'C
Cuts at: 2128 2376 2128
Size: 248 4446
BbsI GAAGACnn'nnnn_
Cuts at: 1081 1364 1081
Size: 283 4411
BbvCI CC'TCA_GC
Cuts at: 1286 1286
Size: 4694
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 111 145 111
Size: 34 4660
BciVI GTATCCnnnnn_n'
Cuts at: 2592 3353 4694 2592
Size: 761 1341 2592
Fragments arranged by size:
2592 1341 761
BclI T'GATC_A
Cuts at: 2214 2214
Size: 4694
BglI GCCn_nnn'nGGC
Cuts at: 1296 1296
Size: 4694
BglII A'GATC_T
Cuts at: 1268 2209 4246 1268
Size: 941 2037 1716
Fragments arranged by size:
2037 1716 941
BlpI GC'TnA_GC
Cuts at: 1678 1806 1678
Size: 128 4566
Bme1580I G_kGCm'C
Cuts at: 219 2319 2412 3468 219
Size: 2100 93 1056 1445
Fragments arranged by size:
2100 1445 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1906 1987 2322 4466 1906
Size: 81 335 2144 2134
Fragments arranged by size:
2144 2134 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1515 3061 1515
Size: 1546 3148
Bpu10I CC'TnA_GC
Cuts at: 1286 1361 1286
Size: 75 4619
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 1304 1870 2749 3241 3539 277
Size: 1027 566 879 492 298 1432
Fragments arranged by size:
1432 1027 879 566 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1187 4398 1187
Size: 3211 1483
BsaAI yAC'GTr
Cuts at: 2047 2047
Size: 4694
BsaBI GATnn'nnATC
Cuts at: 6 1405 2208 4245 6
Size: 1399 803 2037 455
Fragments arranged by size:
2037 1399 803 455
BsaHI Gr'CG_yC
Cuts at: 1086 2125 2373 3075 4571 1086
Size: 1039 248 702 1496 1209
Fragments arranged by size:
1496 1209 1039 702 248
BsaWI w'CCGG_w
Cuts at: 1883 2404 3082 3356 3503 3914 1883
Size: 521 678 274 147 411 2663
Fragments arranged by size:
2663 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 389 419 389
Size: 30 4664
BseYI C'CCAG_C
Cuts at: 1112 1294 1673 2119 2877 3454 3950 1112
Size: 182 379 446 758 577 496 1856
Fragments arranged by size:
1856 758 577 496 446 379 182
BsiEI CG_ry'CG
Cuts at: 104 2236 2282 3490 104
Size: 2132 46 1208 1308
Fragments arranged by size:
2132 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1032 1220 1708 2486 2676 3468 1032
Size: 188 488 778 190 792 2258
Fragments arranged by size:
2258 792 778 488 190 188
Bsp1286I G_dGCh'C
Cuts at: 219 1032 1220 1708 2319 2412 2486 2676 2738
Size: 813 188 488 611 93 74 190 62
Cuts at: 2738 3468 219
Size: 730 1445
Fragments arranged by size:
1445 813 730 611 488 190 188 93
74 62
BspEI T'CCGG_A
Cuts at: 3914 3914
Size: 4694
BspHI T'CATG_A
Cuts at: 380 3870 380
Size: 3490 1204
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1744 1958 2260 2641 1744
Size: 214 302 381 3797
Fragments arranged by size:
3797 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2986 3040 4524 4689 50
Size: 2936 54 1484 165 55
Fragments arranged by size:
2936 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 473 1018 2606 473
Size: 545 1588 2561
Fragments arranged by size:
2561 1588 545
BsrFI r'CCGG_y
Cuts at: 2692 2873 3082 2692
Size: 181 209 4304
Fragments arranged by size:
4304 209 181
BssSI C'ACGA_G
Cuts at: 2965 3323 2965
Size: 358 4336
BstAPI GCAn_nnn'nTGC
Cuts at: 2071 2071
Size: 4694
BstBI TT'CG_AA
Cuts at: 3055 3055
Size: 4694
BstYI r'GATC_y
Cuts at: 1 533 930 1268 1400 1719 2186 2209 2544
Size: 532 397 338 132 319 467 23 335
Cuts at: 2544 2790 3802 3888 3900 4246 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 532 467 449 397 346 338 335
319 246 132 86 23 12
BtgI C'CryG_G
Cuts at: 711 711
Size: 4694
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2644 2825 2988 2644
Size: 181 163 4350
Fragments arranged by size:
4350 181 163
BtsI GCAGTG_nn'
Cuts at: 823 1937 823
Size: 1114 3580
ClaI AT'CG_AT
Cuts at: 1596 1596
Size: 4694
DraI TTT'AAA
Cuts at: 1693 1693
Size: 4694
DraIII CAC_nnn'GTG
Cuts at: 429 429
Size: 4694
DrdI GACnn_nn'nnGTC
Cuts at: 2400 3258 2400
Size: 858 3836
EaeI y'GGCC_r
Cuts at: 2279 2453 2844 2871 4216 2279
Size: 174 391 27 1345 2757
Fragments arranged by size:
2757 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2279 2279
Size: 4694
EarI CTCTTCn'nnn_
Cuts at: 2717 2927 2717
Size: 210 4484
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2902 3212 3358 4504 2902
Size: 310 146 1146 3092
Fragments arranged by size:
3092 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1218 1706 1218
Size: 488 4206
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 713 1358 2519 2951 3698 3966 713
Size: 645 1161 432 747 268 1441
Fragments arranged by size:
1441 1161 747 645 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1833 1833
Size: 4694
EcoRI G'AATT_C
Cuts at: 1431 1698 1431
Size: 267 4427
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1021 2713 1021
Size: 1692 3002
FspI TGC'GCA
Cuts at: 2475 2475
Size: 4694
HaeII r_GCGC'y
Cuts at: 128 2128 2376 3398 4377 128
Size: 2000 248 1022 979 445
Fragments arranged by size:
2000 1022 979 445 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1260 1292 2536 2566 2568 2598 1260
Size: 32 1244 30 2 30 3356
Fragments arranged by size:
3356 1244 32 30 30 2
HincII GTy'rAC
Cuts at: 1530 1733 1530
Size: 203 4491
KasI G'GCGC_C
Cuts at: 2124 2372 2124
Size: 248 4446
KpnI G_GTAC'C
Cuts at: 1714 1714
Size: 4694
MfeI C'AATT_G
Cuts at: 758 758
Size: 4694
MluI A'CGCG_T
Cuts at: 4127 4127
Size: 4694
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 661 1249 3365 3549 661
Size: 588 2116 184 1806
Fragments arranged by size:
2116 1806 588 184
MscI TGG'CCA
Cuts at: 2455 2455
Size: 4694
MslI CAynn'nnrTG
Cuts at: 449 524 542 2683 2810 449
Size: 75 18 2141 127 2333
Fragments arranged by size:
2333 2141 127 75 18
MspA1I CmG'CkG
Cuts at: 1294 1805 2119 2479 3492 3737 1294
Size: 511 314 360 1013 245 2251
Fragments arranged by size:
2251 1013 511 360 314 245
NaeI GCC'GGC
Cuts at: 2875 2875
Size: 4694
NarI GG'CG_CC
Cuts at: 2125 2373 2125
Size: 248 4446
NgoMIV G'CCGG_C
Cuts at: 2873 2873
Size: 4694
NspI r_CATG'y
Cuts at: 1747 2247 3154 1747
Size: 500 907 3287
Fragments arranged by size:
3287 907 500
PciI A'CATG_T
Cuts at: 3150 3150
Size: 4694
PmeI GTTT'AAAC
Cuts at: 1693 1693
Size: 4694
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 389 421 3833 3865 389
Size: 32 3412 32 1218
Fragments arranged by size:
3412 1218 32 32
PspXI vC'TCGA_Gb
Cuts at: 120 120
Size: 4694
PstI C_TGCA'G
Cuts at: 167 830 1741 2426 167
Size: 663 911 685 2435
Fragments arranged by size:
2435 911 685 663
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4694
PvuII CAG'CTG
Cuts at: 1294 2119 2479 1294
Size: 825 360 3509
Fragments arranged by size:
3509 825 360
RsrII CG'GwC_CG
Cuts at: 2889 2889
Size: 4694
SacI G_AGCT'C
Cuts at: 1220 1708 1220
Size: 488 4206
SalI G'TCGA_C
Cuts at: 1528 1731 1528
Size: 203 4491
SapI GCTCTTCn'nnn_
Cuts at: 2717 2927 2717
Size: 210 4484
SbfI CC_TGCA'GG
Cuts at: 1741 1741
Size: 4694
ScaI AGT'ACT
Cuts at: 497 1418 4211 497
Size: 921 2793 980
Fragments arranged by size:
2793 980 921
SexAI A'CCwGG_T
Cuts at: 1071 1071
Size: 4694
SfcI C'TryA_G
Cuts at: 33 163 194 770 826 1737 2422 3415 3606
Size: 130 31 576 56 911 685 993 191
Cuts at: 3606 33
Size: 1121
Fragments arranged by size:
1121 993 911 685 576 191 130 56
31
SfoI GGC'GCC
Cuts at: 2126 2374 2126
Size: 248 4446
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4694
SmaI CCC'GGG
Cuts at: 1716 1716
Size: 4694
SmlI C'TyrA_G
Cuts at: 120 256 1319 1849 2764 3256 3518 120
Size: 136 1063 530 915 492 262 1296
Fragments arranged by size:
1296 1063 915 530 492 262 136
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4694
SphI G_CATG'C
Cuts at: 1747 2247 1747
Size: 500 4194
SspI AAT'ATT
Cuts at: 330 330
Size: 4694
StyI C'CwwG_G
Cuts at: 1828 2182 1828
Size: 354 4340
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1350 2277 2410 2869 3078 1350
Size: 927 133 459 209 2966
Fragments arranged by size:
2966 927 459 209 133
TatI w'GTAC_w
Cuts at: 495 585 1416 4209 495
Size: 90 831 2793 980
Fragments arranged by size:
2793 980 831 90
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4211 4243 4211
Size: 32 4662
Tth111I GACn'n_nGTC
Cuts at: 2491 2491
Size: 4694
XbaI T'CTAG_A
Cuts at: 1725 1725
Size: 4694
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2189 2189
Size: 4694
XhoI C'TCGA_G
Cuts at: 120 120
Size: 4694
XmaI C'CCGG_G
Cuts at: 1714 1714
Size: 4694
XmnI GAAnn'nnTTC
Cuts at: 1004 1004
Size: 4694
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI ClaI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PmeI PpiI PspXI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI ApaI AscI
AvrII BaeI BfrBI BmgBI BmtI BpmI BseRI BsgI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I CspCI EcoNI EcoRV FseI FspAI HindIII HpaI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI StuI SwaI
ZraI