Restriction map for
pF1KA1104
(Circular) (Six-base) MAPSORT of: pF1KA1104.seq Check: 9221 from: 1 to: 6224
pF1KA1104 6224 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AccI GT'mk_AC
Cuts at: 1426 2924 3262 1426
Size: 1498 338 4388
Fragments arranged by size:
4388 1498 338
Acc65I G'GTAC_C
Cuts at: 3240 3240
Size: 6224
AclI AA'CG_TT
Cuts at: 6064 6064
Size: 6224
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 754 2310 2999 4049 4481 4834 5496 754
Size: 1556 689 1050 432 353 662 1482
Fragments arranged by size:
1556 1482 1050 689 662 432 353
AflII C'TTAA_G
Cuts at: 856 856
Size: 6224
AflIII A'CryG_T
Cuts at: 794 1042 3576 5376 5657 794
Size: 248 2534 1800 281 1361
Fragments arranged by size:
2534 1800 1361 281 248
AgeI A'CCGG_T
Cuts at: 491 491
Size: 6224
AleI CACnn'nnGTG
Cuts at: 994 994
Size: 6224
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 884 1012 3104 884
Size: 128 2092 4004
Fragments arranged by size:
4004 2092 128
AlwNI CAG_nnn'CTG
Cuts at: 135 936 1539 3529 4967 135
Size: 801 603 1990 1438 1392
Fragments arranged by size:
1990 1438 1392 801 603
ApaI G_GGCC'C
Cuts at: 1178 1178
Size: 6224
ApaLI G'TGCA_C
Cuts at: 5062 5062
Size: 6224
ApoI r'AATT_y
Cuts at: 944 967 1030 1525 1555 2246 2785 2801 2944
Size: 23 63 495 30 691 539 16 143
Cuts at: 2944 3228 5577 5833 944
Size: 284 2349 256 1335
Fragments arranged by size:
2349 1335 691 539 495 284 256 143
63 30 23 16
AseI AT'TA_AT
Cuts at: 20 20
Size: 6224
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6224
AvaI C'yCGr_G
Cuts at: 280 1930 3175 3244 280
Size: 1650 1245 69 3260
Fragments arranged by size:
3260 1650 1245 69
AvrII C'CTAG_G
Cuts at: 1024 1024
Size: 6224
BamHI G'GATC_C
Cuts at: 3249 3249
Size: 6224
BanI G'GyrC_C
Cuts at: 996 2261 3240 3654 3902 3937 996
Size: 1265 979 414 248 35 3283
Fragments arranged by size:
3283 1265 979 414 248 35
BanII G_rGCy'C
Cuts at: 219 1178 1499 2237 3238 4268 219
Size: 959 321 738 1001 1030 2175
Fragments arranged by size:
2175 1030 1001 959 738 321
BbeI G_GCGC'C
Cuts at: 3658 3906 3658
Size: 248 5976
BbsI GAAGACnn'nnnn_
Cuts at: 359 1141 1148 2038 2504 2674 2976 359
Size: 782 7 890 466 170 302 3607
Fragments arranged by size:
3607 890 782 466 302 170 7
BbvCI CC'TCA_GC
Cuts at: 145 1939 2465 145
Size: 1794 526 3904
Fragments arranged by size:
3904 1794 526
BciVI GTATCCnnnnn_n'
Cuts at: 554 4122 5178 6224 554
Size: 3568 1056 1046 554
Fragments arranged by size:
3568 1056 1046 554
BclI T'GATC_A
Cuts at: 3744 3744
Size: 6224
BfrBI ATG'CAT
Cuts at: 2714 2714
Size: 6224
BglII A'GATC_T
Cuts at: 1715 3739 5776 1715
Size: 2024 2037 2163
Fragments arranged by size:
2163 2037 2024
BlpI GC'TnA_GC
Cuts at: 154 2015 3336 154
Size: 1861 1321 3042
Fragments arranged by size:
3042 1861 1321
Bme1580I G_kGCm'C
Cuts at: 1178 1955 2264 2576 3849 3942 5066 1178
Size: 777 309 312 1273 93 1124 2336
Fragments arranged by size:
2336 1273 1124 777 312 309 93
BmgBI CAC'GTC
Cuts at: 1833 1833
Size: 6224
BmrI ACTGGGnnnn_n'
Cuts at: 2937 3149 3436 3517 3852 5996 2937
Size: 212 287 81 335 2144 3165
Fragments arranged by size:
3165 2144 335 287 212 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2069 3100 4591 2069
Size: 1031 1491 3702
Fragments arranged by size:
3702 1491 1031
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1868 1896 2887 1868
Size: 28 991 5205
Fragments arranged by size:
5205 991 28
Bpu10I CC'TnA_GC
Cuts at: 145 1939 2465 145
Size: 1794 526 3904
Fragments arranged by size:
3904 1794 526
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 445 1657 1745 3400 4279 4993 5291 445
Size: 1212 88 1655 879 714 298 1378
Fragments arranged by size:
1655 1378 1212 879 714 298 88
BsaI GGTCTCn'nnnn_
Cuts at: 452 461 813 1306 2314 5928 452
Size: 9 352 493 1008 3614 748
Fragments arranged by size:
3614 1008 748 493 352 9
BsaAI yAC'GTr
Cuts at: 797 908 2061 2407 2703 3577 797
Size: 111 1153 346 296 874 3444
Fragments arranged by size:
3444 1153 874 346 296 111
BsaBI GATnn'nnATC
Cuts at: 6 3738 5775 6
Size: 3732 2037 455
Fragments arranged by size:
3732 2037 455
BsaHI Gr'CG_yC
Cuts at: 688 3655 3903 4605 6101 688
Size: 2967 248 702 1496 811
Fragments arranged by size:
2967 1496 811 702 248
BsaWI w'CCGG_w
Cuts at: 491 1110 2035 3413 3934 4612 5023 5170 5444
Size: 619 925 1378 521 678 411 147 274
Cuts at: 5444 491
Size: 1271
Fragments arranged by size:
1378 1271 925 678 619 521 411 274
147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 574 1948 2103 2209 2453 574
Size: 1374 155 106 244 4345
Fragments arranged by size:
4345 1374 244 155 106
BseYI C'CCAG_C
Cuts at: 761 1874 1980 2575 3649 4407 5072 5480 761
Size: 1113 106 595 1074 758 665 408 1505
Fragments arranged by size:
1505 1113 1074 758 665 595 408 106
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 483 397
Size: 86 6138
BsiEI CG_ry'CG
Cuts at: 104 2495 2520 2561 3766 3812 5042 104
Size: 2391 25 41 1205 46 1230 1286
Fragments arranged by size:
2391 1286 1230 1205 46 41 25
BsiHKAI G_wGCw'C
Cuts at: 1499 2066 2201 2237 3151 3238 4016 4206 5066
Size: 567 135 36 914 87 778 190 860
Cuts at: 5066 1499
Size: 2657
Fragments arranged by size:
2657 914 860 778 567 190 135 87
36
BsiWI C'GTAC_G
Cuts at: 908 908
Size: 6224
BsmI GAATG_Cn'
Cuts at: 2460 2716 2460
Size: 256 5968
BsmBI CGTCTCn'nnnn_
Cuts at: 2890 2890
Size: 6224
Bsp1286I G_dGCh'C
Cuts at: 219 1178 1499 1955 2066 2201 2237 2264 2576
Size: 959 321 456 111 135 36 27 312
Cuts at: 2576 3151 3238 3849 3942 4016 4206 4268 5066
Size: 575 87 611 93 74 190 62 798
Cuts at: 5066 219
Size: 1377
Fragments arranged by size:
1377 959 798 611 575 456 321 312
190 135 111 93 87 74 62 36
27
BspHI T'CATG_A
Cuts at: 509 4656 509
Size: 4147 2077
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2144 2321 3274 3488 3790 4171 2144
Size: 177 953 214 302 381 4197
Fragments arranged by size:
4197 953 381 302 214 177
BsrBI CCG'CTC
Cuts at: 50 158 4516 4570 6054 6219 50
Size: 108 4358 54 1484 165 55
Fragments arranged by size:
4358 1484 165 108 55 54
BsrDI GCAATG_nn'
Cuts at: 897 4136 897
Size: 3239 2985
BsrFI r'CCGG_y
Cuts at: 491 4222 4403 491
Size: 3731 181 2312
Fragments arranged by size:
3731 2312 181
BssSI C'ACGA_G
Cuts at: 949 1257 1639 4495 5203 949
Size: 308 382 2856 708 1970
Fragments arranged by size:
2856 1970 708 382 308
BstAPI GCAn_nnn'nTGC
Cuts at: 2103 3601 2103
Size: 1498 4726
BstBI TT'CG_AA
Cuts at: 4585 4585
Size: 6224
BstEII G'GTnAC_C
Cuts at: 817 817
Size: 6224
BstXI CCAn_nnnn'nTGG
Cuts at: 396 1890 2911 396
Size: 1494 1021 3709
Fragments arranged by size:
3709 1494 1021
BstYI r'GATC_y
Cuts at: 1 242 1068 1715 3249 3716 3739 4074 4320
Size: 241 826 647 1534 467 23 335 246
Cuts at: 4320 4626 4638 4724 5776 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1534 1052 826 647 467 449 335 306
246 241 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2392 2392
Size: 6224
BtgI C'CryG_G
Cuts at: 246 395 992 2607 246
Size: 149 597 1615 3863
Fragments arranged by size:
3863 1615 597 149
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 201 4174 4355 4518 201
Size: 3973 181 163 1907
Fragments arranged by size:
3973 1907 181 163
BtsI GCAGTG_nn'
Cuts at: 305 3467 305
Size: 3162 3062
ClaI AT'CG_AT
Cuts at: 1615 1615
Size: 6224
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 974 1009 974
Size: 35 6189
DraI TTT'AAA
Cuts at: 702 3223 702
Size: 2521 3703
DrdI GACnn_nn'nnGTC
Cuts at: 2904 3930 5274 2904
Size: 1026 1344 3854
Fragments arranged by size:
3854 1344 1026
EaeI y'GGCC_r
Cuts at: 344 3113 3161 3809 3983 4374 4401 5746 344
Size: 2769 48 648 174 391 27 1345 822
Fragments arranged by size:
2769 1345 822 648 391 174 48 27
EagI C'GGCC_G
Cuts at: 3809 3809
Size: 6224
EarI CTCTTCn'nnn_
Cuts at: 1963 2186 2350 3139 4247 4457 1963
Size: 223 164 789 1108 210 3730
Fragments arranged by size:
3730 1108 789 223 210 164
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 176 4432 5174 5320 6034 176
Size: 4256 742 146 714 366
Fragments arranged by size:
4256 742 714 366 146
EcoICRI GAG'CTC
Cuts at: 1497 2235 3236 1497
Size: 738 1001 4485
Fragments arranged by size:
4485 1001 738
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 754 1868 1896 2310 2887 2999 4049 4481 4834
Size: 1114 28 414 577 112 1050 432 353
Cuts at: 4834 5496 754
Size: 662 1482
Fragments arranged by size:
1482 1114 1050 662 577 432 414 353
112 28
EcoO109I rG'GnC_Cy
Cuts at: 132 1485 1943 2310 2880 3198 3363 132
Size: 1353 458 367 570 318 165 2993
Fragments arranged by size:
2993 1353 570 458 367 318 165
EcoRI G'AATT_C
Cuts at: 1030 2246 3228 1030
Size: 1216 982 4026
Fragments arranged by size:
4026 1216 982
EcoRV GAT'ATC
Cuts at: 1854 2379 1854
Size: 525 5699
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2436 2521 2691 2992 4243 2436
Size: 85 170 301 1251 4417
Fragments arranged by size:
4417 1251 301 170 85
FspI TGC'GCA
Cuts at: 4005 4005
Size: 6224
HaeII r_GCGC'y
Cuts at: 117 3658 3906 5136 5907 117
Size: 3541 248 1230 771 434
Fragments arranged by size:
3541 1230 771 434 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 227 259 1404 1436 1678 1710 2655 2687 4066
Size: 32 1145 32 242 32 945 32 1379
Cuts at: 4066 4096 4098 4128 227
Size: 30 2 30 2323
Fragments arranged by size:
2323 1379 1145 945 242 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1747 2925 3263 1747
Size: 1178 338 4708
Fragments arranged by size:
4708 1178 338
KasI G'GCGC_C
Cuts at: 3654 3902 3654
Size: 248 5976
KpnI G_GTAC'C
Cuts at: 3244 3244
Size: 6224
MluI A'CGCG_T
Cuts at: 5657 5657
Size: 6224
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1221 1808 2500 4983 5167 1221
Size: 587 692 2483 184 2278
Fragments arranged by size:
2483 2278 692 587 184
MscI TGG'CCA
Cuts at: 346 3163 3985 346
Size: 2817 822 2585
Fragments arranged by size:
2817 2585 822
MslI CAynn'nnrTG
Cuts at: 394 508 994 4213 4340 394
Size: 114 486 3219 127 2278
Fragments arranged by size:
3219 2278 486 127 114
MspA1I CmG'CkG
Cuts at: 1846 2330 3335 3649 4009 4793 5038 1846
Size: 484 1005 314 360 784 245 3032
Fragments arranged by size:
3032 1005 784 484 360 314 245
NaeI GCC'GGC
Cuts at: 4405 4405
Size: 6224
NarI GG'CG_CC
Cuts at: 3655 3903 3655
Size: 248 5976
NcoI C'CATG_G
Cuts at: 246 395 2607 246
Size: 149 2212 3863
Fragments arranged by size:
3863 2212 149
NgoMIV G'CCGG_C
Cuts at: 4403 4403
Size: 6224
NsiI A_TGCA'T
Cuts at: 2716 2716
Size: 6224
NspI r_CATG'y
Cuts at: 169 1046 3277 3777 5380 169
Size: 877 2231 500 1603 1013
Fragments arranged by size:
2231 1603 1013 877 500
PasI CC'CwG_GG
Cuts at: 1013 1013
Size: 6224
PciI A'CATG_T
Cuts at: 1042 5376 1042
Size: 4334 1890
PfoI T'CCnGG_A
Cuts at: 833 3205 833
Size: 2372 3852
PmeI GTTT'AAAC
Cuts at: 3223 3223
Size: 6224
PmlI CAC'GTG
Cuts at: 2061 2061
Size: 6224
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4670 4702 4670
Size: 32 6192
PpuMI rG'GwC_Cy
Cuts at: 1485 2310 2880 3198 1485
Size: 825 570 318 4511
Fragments arranged by size:
4511 825 570 318
PspOMI G'GGCC_C
Cuts at: 1174 1174
Size: 6224
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1796 1828 1796
Size: 32 6192
PstI C_TGCA'G
Cuts at: 224 300 1544 1966 2318 3271 3956 224
Size: 76 1244 422 352 953 685 2492
Fragments arranged by size:
2492 1244 953 685 422 352 76
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6224
PvuII CAG'CTG
Cuts at: 1846 3649 4009 1846
Size: 1803 360 4061
Fragments arranged by size:
4061 1803 360
RsrII CG'GwC_CG
Cuts at: 4419 4419
Size: 6224
SacI G_AGCT'C
Cuts at: 1499 2237 3238 1499
Size: 738 1001 4485
Fragments arranged by size:
4485 1001 738
SalI G'TCGA_C
Cuts at: 2923 3261 2923
Size: 338 5886
SanDI GG'GwC_CC
Cuts at: 1485 2880 1485
Size: 1395 4829
SapI GCTCTTCn'nnn_
Cuts at: 3139 4247 4457 3139
Size: 1108 210 4906
Fragments arranged by size:
4906 1108 210
SbfI CC_TGCA'GG
Cuts at: 1544 2318 3271 1544
Size: 774 953 4497
Fragments arranged by size:
4497 953 774
ScaI AGT'ACT
Cuts at: 405 1867 5741 405
Size: 1462 3874 888
Fragments arranged by size:
3874 1462 888
SexAI A'CCwGG_T
Cuts at: 263 263
Size: 6224
SfcI C'TryA_G
Cuts at: 33 220 296 1064 1540 1962 2314 3267 3952
Size: 187 76 768 476 422 352 953 685
Cuts at: 3952 4920 5111 33
Size: 968 191 1146
Fragments arranged by size:
1146 968 953 768 685 476 422 352
191 187 76
SfoI GGC'GCC
Cuts at: 3656 3904 3656
Size: 248 5976
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6224
SmaI CCC'GGG
Cuts at: 3246 3246
Size: 6224
SmlI C'TyrA_G
Cuts at: 424 856 1672 1760 3379 4294 5008 5270 424
Size: 432 816 88 1619 915 714 262 1378
Fragments arranged by size:
1619 1378 915 816 714 432 262 88
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6224
SphI G_CATG'C
Cuts at: 3277 3777 3277
Size: 500 5724
SspI AAT'ATT
Cuts at: 2168 2168
Size: 6224
StuI AGG'CCT
Cuts at: 1267 2541 1267
Size: 1274 4950
StyI C'CwwG_G
Cuts at: 246 395 566 692 1024 1215 1287 2607 3194
Size: 149 171 126 332 191 72 1320 587
Cuts at: 3194 3358 3712 246
Size: 164 354 2758
Fragments arranged by size:
2758 1320 587 354 332 191 171 164
149 126 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 899 2506 2695 3807 3940 4399 4608 325
Size: 574 1607 189 1112 133 459 209 1941
Fragments arranged by size:
1941 1607 1112 574 459 209 189 133
TatI w'GTAC_w
Cuts at: 403 1865 5739 403
Size: 1462 3874 888
Fragments arranged by size:
3874 1462 888
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 546 578 815 847 5741 5773 546
Size: 32 237 32 4894 32 997
Fragments arranged by size:
4894 997 237 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1834 4021 1834
Size: 2187 4037
XbaI T'CTAG_A
Cuts at: 1731 3255 1731
Size: 1524 4700
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1042 1882 1887 2605 3719 1042
Size: 840 5 718 1114 3547
Fragments arranged by size:
3547 1114 840 718 5
XmaI C'CCGG_G
Cuts at: 3244 3244
Size: 6224
XmnI GAAnn'nnTTC
Cuts at: 517 517
Size: 6224
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI
PciI PfoI PmeI PmlI PpiI PpuMI PspOMI PsrI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AloI AscI BaeI BcgI
BglI BmtI BsaXI BspEI BsrGI BssHII BstZ17I DraIII
EcoNI FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI PacI PflMI PshAI PsiI PspXI SacII
SfiI SgrAI SnaBI SrfI SwaI XhoI ZraI