Restriction map for
pF1KA1106
(Circular) (Six-base) MAPSORT of: pF1KA1106.seq Check: 1285 from: 1 to: 6509
pF1KA1106 6509 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AccI GT'mk_AC
Cuts at: 2408 3421 3547 2408
Size: 1013 126 5370
Fragments arranged by size:
5370 1013 126
Acc65I G'GTAC_C
Cuts at: 2909 3525 2909
Size: 616 5893
AclI AA'CG_TT
Cuts at: 6349 6349
Size: 6509
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 416 1232 3265 4334 4766 5513 5781 416
Size: 816 2033 1069 432 747 268 1144
Fragments arranged by size:
2033 1144 1069 816 747 432 268
AflIII A'CryG_T
Cuts at: 1724 2852 3861 4965 5942 1724
Size: 1128 1009 1104 977 2291
Fragments arranged by size:
2291 1128 1104 1009 977
AgeI A'CCGG_T
Cuts at: 2363 4897 2363
Size: 2534 3975
AhdI GACnn_n'nnGTC
Cuts at: 816 816
Size: 6509
AleI CACnn'nnGTG
Cuts at: 257 1192 257
Size: 935 5574
AlwNI CAG_nnn'CTG
Cuts at: 2193 2214 2303 3090 3327 3814 5381 2193
Size: 21 89 787 237 487 1567 3321
Fragments arranged by size:
3321 1567 787 487 237 89 21
ApaLI G'TGCA_C
Cuts at: 5279 5279
Size: 6509
ApoI r'AATT_y
Cuts at: 3384 3494 3513 5862 6118 3384
Size: 110 19 2349 256 3775
Fragments arranged by size:
3775 2349 256 110 19
AseI AT'TA_AT
Cuts at: 20 20
Size: 6509
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6509
AvaI C'yCGr_G
Cuts at: 1372 1471 1858 2515 2815 3039 3529 1372
Size: 99 387 657 300 224 490 4352
Fragments arranged by size:
4352 657 490 387 300 224 99
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2533 2566 2533
Size: 33 6476
BamHI G'GATC_C
Cuts at: 3366 3534 3366
Size: 168 6341
BanI G'GyrC_C
Cuts at: 2876 2909 3525 3939 4187 4222 2876
Size: 33 616 414 248 35 5163
Fragments arranged by size:
5163 616 414 248 35 33
BanII G_rGCy'C
Cuts at: 1123 1859 2330 3523 4553 1123
Size: 736 471 1193 1030 3079
Fragments arranged by size:
3079 1193 1030 736 471
BbeI G_GCGC'C
Cuts at: 3943 4191 3943
Size: 248 6261
BbsI GAAGACnn'nnnn_
Cuts at: 404 464 659 2477 3253 404
Size: 60 195 1818 776 3660
Fragments arranged by size:
3660 1818 776 195 60
BbvCI CC'TCA_GC
Cuts at: 587 2269 587
Size: 1682 4827
BciVI GTATCCnnnnn_n'
Cuts at: 2522 4407 5168 6509 2522
Size: 1885 761 1341 2522
Fragments arranged by size:
2522 1885 1341 761
BclI T'GATC_A
Cuts at: 4029 4029
Size: 6509
BfrBI ATG'CAT
Cuts at: 1575 1575
Size: 6509
BglI GCCn_nnn'nGGC
Cuts at: 604 2137 604
Size: 1533 4976
BglII A'GATC_T
Cuts at: 4024 6061 4024
Size: 2037 4472
BlpI GC'TnA_GC
Cuts at: 3621 3621
Size: 6509
Bme1580I G_kGCm'C
Cuts at: 1597 2662 2881 4134 4227 5283 1597
Size: 1065 219 1253 93 1056 2823
Fragments arranged by size:
2823 1253 1065 1056 219 93
BmgBI CAC'GTC
Cuts at: 1725 2997 3050 1725
Size: 1272 53 5184
Fragments arranged by size:
5184 1272 53
BmrI ACTGGGnnnn_n'
Cuts at: 1508 2852 2919 3721 3802 4137 6281 1508
Size: 1344 67 802 81 335 2144 1736
Fragments arranged by size:
2144 1736 1344 802 335 81 67
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3341 4876 3341
Size: 1535 4974
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 952 1126 1273 1541 1807 2344 2450 3098 952
Size: 174 147 268 266 537 106 648 4363
Fragments arranged by size:
4363 648 537 268 266 174 147 106
Bpu10I CC'TnA_GC
Cuts at: 587 1759 2053 2194 2269 2988 3316 587
Size: 1172 294 141 75 719 328 3780
Fragments arranged by size:
3780 1172 719 328 294 141 75
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1419 1573 2638 3685 4564 5056 5354 1419
Size: 154 1065 1047 879 492 298 2574
Fragments arranged by size:
2574 1065 1047 879 492 298 154
BsaI GGTCTCn'nnnn_
Cuts at: 973 6213 973
Size: 5240 1269
BsaAI yAC'GTr
Cuts at: 1494 2087 3399 3862 1494
Size: 593 1312 463 4141
Fragments arranged by size:
4141 1312 593 463
BsaBI GATnn'nnATC
Cuts at: 6 3437 4023 6060 6
Size: 3431 586 2037 455
Fragments arranged by size:
3431 2037 586 455
BsaHI Gr'CG_yC
Cuts at: 1063 3940 4188 4890 6386 1063
Size: 2877 248 702 1496 1186
Fragments arranged by size:
2877 1496 1186 702 248
BsaWI w'CCGG_w
Cuts at: 1528 1660 2363 2808 3698 4219 4897 5171 5318
Size: 132 703 445 890 521 678 274 147
Cuts at: 5318 5729 1528
Size: 411 2308
Fragments arranged by size:
2308 890 703 678 521 445 411 274
147 132
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1099 1129 1147 1177 1627 1657 2317 2347 1099
Size: 30 18 30 450 30 660 30 5261
Fragments arranged by size:
5261 660 450 30 30 30 30 18
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 139 351 430 445 448 913 925 1123 1338
Size: 212 79 15 3 465 12 198 215
Cuts at: 1338 3274 3286 139
Size: 1936 12 3362
Fragments arranged by size:
3362 1936 465 215 212 198 79 15
12 12 3
BseYI C'CCAG_C
Cuts at: 1318 1999 2005 2035 2339 2376 2393 3934 4692
Size: 681 6 30 304 37 17 1541 758
Cuts at: 4692 5269 5765 1318
Size: 577 496 2062
Fragments arranged by size:
2062 1541 758 681 577 496 304 37
30 17 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1593 2109 1593
Size: 516 5993
BsiEI CG_ry'CG
Cuts at: 104 1129 1673 4051 4097 5305 104
Size: 1025 544 2378 46 1208 1308
Fragments arranged by size:
2378 1308 1208 1025 544 46
BsiHKAI G_wGCw'C
Cuts at: 779 1760 1859 2200 3523 4301 4491 5283 779
Size: 981 99 341 1323 778 190 792 2005
Fragments arranged by size:
2005 1323 981 792 778 341 190 99
BsmI GAATG_Cn'
Cuts at: 2618 2618
Size: 6509
Bsp1286I G_dGCh'C
Cuts at: 779 1123 1597 1760 1859 2200 2330 2662 2881
Size: 344 474 163 99 341 130 332 219
Cuts at: 2881 3523 4134 4227 4301 4491 4553 5283 779
Size: 642 611 93 74 190 62 730 2005
Fragments arranged by size:
2005 730 642 611 474 344 341 332
219 190 163 130 99 93 74 62
BspEI T'CCGG_A
Cuts at: 1528 1660 5729 1528
Size: 132 4069 2308
Fragments arranged by size:
4069 2308 132
BspHI T'CATG_A
Cuts at: 1167 5685 1167
Size: 4518 1991
BspMI ACCTGCnnnn'nnnn_
Cuts at: 327 3559 3773 4075 4456 327
Size: 3232 214 302 381 2380
Fragments arranged by size:
3232 2380 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1173 4801 4855 6339 6504 50
Size: 1123 3628 54 1484 165 55
Fragments arranged by size:
3628 1484 1123 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4421 4421
Size: 6509
BsrFI r'CCGG_y
Cuts at: 1486 2363 2695 4507 4688 4897 1486
Size: 877 332 1812 181 209 3098
Fragments arranged by size:
3098 1812 877 332 209 181
BssSI C'ACGA_G
Cuts at: 3301 4780 5138 3301
Size: 1479 358 4672
Fragments arranged by size:
4672 1479 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2087 2963 3886 2087
Size: 876 923 4710
Fragments arranged by size:
4710 923 876
BstBI TT'CG_AA
Cuts at: 2622 4870 2622
Size: 2248 4261
BstEII G'GTnAC_C
Cuts at: 2523 2523
Size: 6509
BstXI CCAn_nnnn'nTGG
Cuts at: 114 648 1269 1498 1912 2842 114
Size: 534 621 229 414 930 3781
Fragments arranged by size:
3781 930 621 534 414 229
BstYI r'GATC_y
Cuts at: 1 3366 3534 4001 4024 4359 4605 5617 5703
Size: 3365 168 467 23 335 246 1012 86
Cuts at: 5703 5715 6061 1
Size: 12 346 449
Fragments arranged by size:
3365 1012 467 449 346 335 246 168
86 23 12
BstZ17I GTA'TAC
Cuts at: 3422 3422
Size: 6509
BtgI C'CryG_G
Cuts at: 107 167 1310 2290 107
Size: 60 1143 980 4326
Fragments arranged by size:
4326 1143 980 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 121 920 1248 2845 3070 4459 4640 4803 121
Size: 799 328 1597 225 1389 181 163 1827
Fragments arranged by size:
1827 1597 1389 799 328 225 181 163
BtsI GCAGTG_nn'
Cuts at: 228 2357 3752 228
Size: 2129 1395 2985
Fragments arranged by size:
2985 2129 1395
DraI TTT'AAA
Cuts at: 3508 3508
Size: 6509
DrdI GACnn_nn'nnGTC
Cuts at: 1408 4215 5073 1408
Size: 2807 858 2844
Fragments arranged by size:
2844 2807 858
EaeI y'GGCC_r
Cuts at: 554 962 1036 1307 1488 1691 1847 2141 2633
Size: 408 74 271 181 203 156 294 492
Cuts at: 2633 2826 2888 4094 4268 4659 4686 6031 554
Size: 193 62 1206 174 391 27 1345 1032
Fragments arranged by size:
1345 1206 1032 492 408 391 294 271
203 193 181 174 156 74 62 27
EagI C'GGCC_G
Cuts at: 4094 4094
Size: 6509
EarI CTCTTCn'nnn_
Cuts at: 434 1037 1208 3251 4532 4742 434
Size: 603 171 2043 1281 210 2201
Fragments arranged by size:
2201 2043 1281 603 210 171
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2623 4717 5027 5173 6319 2623
Size: 2094 310 146 1146 2813
Fragments arranged by size:
2813 2094 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1857 3521 1857
Size: 1664 4845
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 416 952 1126 1232 1273 1541 1807 2344 2450
Size: 536 174 106 41 268 266 537 106
Cuts at: 2450 3098 3265 4334 4766 5513 5781 416
Size: 648 167 1069 432 747 268 1144
Fragments arranged by size:
1144 1069 747 648 537 536 432 268
268 266 174 167 106 106 41
EcoNI CCTnn'n_nnAGG
Cuts at: 1849 1849
Size: 6509
EcoO109I rG'GnC_Cy
Cuts at: 1551 1995 2211 2258 3648 1551
Size: 444 216 47 1390 4412
Fragments arranged by size:
4412 1390 444 216 47
EcoRI G'AATT_C
Cuts at: 3494 3513 3494
Size: 19 6490
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2118 4528 2118
Size: 2410 4099
FspI TGC'GCA
Cuts at: 4290 4290
Size: 6509
HaeII r_GCGC'y
Cuts at: 2078 2870 3943 4191 5213 6192 2078
Size: 792 1073 248 1022 979 2395
Fragments arranged by size:
2395 1073 1022 979 792 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 346 378 802 834 1174 1206 1339 1371 1446
Size: 32 424 32 340 32 133 32 75
Cuts at: 1446 1478 1655 1687 2317 2349 2434 2466 4351
Size: 32 177 32 630 32 85 32 1885
Cuts at: 4351 4381 4383 4413 346
Size: 30 2 30 2442
Fragments arranged by size:
2442 1885 630 424 340 177 133 85
75 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1629 3548 1629
Size: 1919 4590
HindIII A'AGCT_T
Cuts at: 3070 3070
Size: 6509
KasI G'GCGC_C
Cuts at: 3939 4187 3939
Size: 248 6261
KpnI G_GTAC'C
Cuts at: 2913 3529 2913
Size: 616 5893
MluI A'CGCG_T
Cuts at: 2852 5942 2852
Size: 3090 3419
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 664 1489 1865 5180 5364 664
Size: 825 376 3315 184 1809
Fragments arranged by size:
3315 1809 825 376 184
MscI TGG'CCA
Cuts at: 556 964 1309 1693 1849 2635 4270 556
Size: 408 345 384 156 786 1635 2795
Fragments arranged by size:
2795 1635 786 408 384 345 156
MslI CAynn'nnrTG
Cuts at: 112 233 257 1192 1580 1910 2242 2419 4498
Size: 121 24 935 388 330 332 177 2079
Cuts at: 4498 4625 112
Size: 127 1996
Fragments arranged by size:
2079 1996 935 388 332 330 177 127
121 24
MspA1I CmG'CkG
Cuts at: 197 1145 1785 2292 2300 2376 3002 3105 3354
Size: 948 640 507 8 76 626 103 249
Cuts at: 3354 3620 3934 4294 5307 5552 197
Size: 266 314 360 1013 245 1154
Fragments arranged by size:
1154 1013 948 640 626 507 360 314
266 249 245 103 76 8
NaeI GCC'GGC
Cuts at: 4690 4690
Size: 6509
NarI GG'CG_CC
Cuts at: 3940 4188 3940
Size: 248 6261
NcoI C'CATG_G
Cuts at: 107 1310 107
Size: 1203 5306
NdeI CA'TA_TG
Cuts at: 1344 1344
Size: 6509
NgoMIV G'CCGG_C
Cuts at: 4688 4688
Size: 6509
NsiI A_TGCA'T
Cuts at: 1577 1577
Size: 6509
NspI r_CATG'y
Cuts at: 819 1033 1089 1627 2961 3562 4062 4969 819
Size: 214 56 538 1334 601 500 907 2359
Fragments arranged by size:
2359 1334 907 601 538 500 214 56
PciI A'CATG_T
Cuts at: 4965 4965
Size: 6509
PflMI CCAn_nnn'nTGG
Cuts at: 3306 3339 3306
Size: 33 6476
PfoI T'CCnGG_A
Cuts at: 2486 2932 2486
Size: 446 6063
PmeI GTTT'AAAC
Cuts at: 3508 3508
Size: 6509
PmlI CAC'GTG
Cuts at: 2087 2087
Size: 6509
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5648 5680 5648
Size: 32 6477
PpuMI rG'GwC_Cy
Cuts at: 1551 1995 2211 2258 1551
Size: 444 216 47 5802
Fragments arranged by size:
5802 444 216 47
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 164 196 3207 3239 164
Size: 32 3011 32 3434
Fragments arranged by size:
3434 3011 32 32
PstI C_TGCA'G
Cuts at: 1680 3556 4241 1680
Size: 1876 685 3948
Fragments arranged by size:
3948 1876 685
PvuI CG_AT'CG
Cuts at: 104 1673 104
Size: 1569 4940
PvuII CAG'CTG
Cuts at: 197 1145 1785 2300 2376 3354 3934 4294 197
Size: 948 640 515 76 978 580 360 2412
Fragments arranged by size:
2412 978 948 640 580 515 360 76
RsrII CG'GwC_CG
Cuts at: 1749 4704 1749
Size: 2955 3554
SacI G_AGCT'C
Cuts at: 1859 3523 1859
Size: 1664 4845
SacII CC_GC'GG
Cuts at: 2293 2293
Size: 6509
SalI G'TCGA_C
Cuts at: 3546 3546
Size: 6509
SanDI GG'GwC_CC
Cuts at: 1551 2258 1551
Size: 707 5802
SapI GCTCTTCn'nnn_
Cuts at: 4532 4742 4532
Size: 210 6299
SbfI CC_TGCA'GG
Cuts at: 3556 3556
Size: 6509
ScaI AGT'ACT
Cuts at: 6026 6026
Size: 6509
SfcI C'TryA_G
Cuts at: 33 1676 1806 3552 4237 5230 5421 33
Size: 1643 130 1746 685 993 191 1121
Fragments arranged by size:
1746 1643 1121 993 685 191 130
SfoI GGC'GCC
Cuts at: 3941 4189 3941
Size: 248 6261
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6509
SmaI CCC'GGG
Cuts at: 1374 1473 2517 2817 3531 1374
Size: 99 1044 300 714 4352
Fragments arranged by size:
4352 1044 714 300 99
SmlI C'TyrA_G
Cuts at: 1434 1588 1858 2653 3664 4579 5071 5333 1434
Size: 154 270 795 1011 915 492 262 2610
Fragments arranged by size:
2610 1011 915 795 492 270 262 154
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6509
SphI G_CATG'C
Cuts at: 3562 4062 3562
Size: 500 6009
SspI AAT'ATT
Cuts at: 1867 1867
Size: 6509
StuI AGG'CCT
Cuts at: 1744 1744
Size: 6509
StyI C'CwwG_G
Cuts at: 107 275 759 1232 1310 1694 1850 1934 2882
Size: 168 484 473 78 384 156 84 948
Cuts at: 2882 3643 3997 107
Size: 761 354 2619
Fragments arranged by size:
2619 948 761 484 473 384 354 168
156 84 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1151 2931 4092 4225 4684 4893 1151
Size: 1780 1161 133 459 209 2767
Fragments arranged by size:
2767 1780 1161 459 209 133
TatI w'GTAC_w
Cuts at: 6024 6024
Size: 6509
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 960 992 1147 1179 6026 6058 960
Size: 32 155 32 4847 32 1411
Fragments arranged by size:
4847 1411 155 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 615 4306 615
Size: 3691 2818
XbaI T'CTAG_A
Cuts at: 2496 3540 2496
Size: 1044 5465
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3186 4004 3186
Size: 818 5691
XhoI C'TCGA_G
Cuts at: 1858 1858
Size: 6509
XmaI C'CCGG_G
Cuts at: 1372 1471 2515 2815 3529 1372
Size: 99 1044 300 714 4352
Fragments arranged by size:
4352 1044 714 300 99
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NsiI NspI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI AloI ApaI AscI
AvrII BcgI BmtI BsiWI BsmBI BsrGI BssHII Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HpaI MfeI
NheI NotI NruI PacI PasI PshAI PsiI PspOMI
PspXI SexAI SfiI SgrAI SnaBI SrfI SwaI XmnI
ZraI