Restriction map for
pF1KA1111
(Circular) (Six-base) MAPSORT of: pF1KA1111.seq Check: 5078 from: 1 to: 6293
pF1KA1111 6293 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2304 2304
Size: 6293
AccI GT'mk_AC
Cuts at: 2003 3331 2003
Size: 1328 4965
Acc65I G'GTAC_C
Cuts at: 983 3309 983
Size: 2326 3967
AclI AA'CG_TT
Cuts at: 1556 6133 1556
Size: 4577 1716
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 547 561 619 1275 1625 1720 1834 2935 3152
Size: 14 58 656 350 95 114 1101 217
Cuts at: 3152 4118 4550 5297 5565 547
Size: 966 432 747 268 1275
Fragments arranged by size:
1275 1101 966 747 656 432 350 268
217 114 95 58 14
AflIII A'CryG_T
Cuts at: 141 287 3645 4749 5726 141
Size: 146 3358 1104 977 708
Fragments arranged by size:
3358 1104 977 708 146
AgeI A'CCGG_T
Cuts at: 4681 4681
Size: 6293
AleI CACnn'nnGTG
Cuts at: 1642 1642
Size: 6293
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 640 640
Size: 6293
AlwNI CAG_nnn'CTG
Cuts at: 1812 2845 3598 5165 1812
Size: 1033 753 1567 2940
Fragments arranged by size:
2940 1567 1033 753
ApaI G_GGCC'C
Cuts at: 392 392
Size: 6293
ApaLI G'TGCA_C
Cuts at: 5063 5063
Size: 6293
ApoI r'AATT_y
Cuts at: 556 796 2044 2125 2724 3297 5646 5902 556
Size: 240 1248 81 599 573 2349 256 947
Fragments arranged by size:
2349 1248 947 599 573 256 240 81
AseI AT'TA_AT
Cuts at: 20 20
Size: 6293
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6293
AvaI C'yCGr_G
Cuts at: 2807 3313 2807
Size: 506 5787
BamHI G'GATC_C
Cuts at: 1166 3318 1166
Size: 2152 4141
BanI G'GyrC_C
Cuts at: 225 970 983 1855 3161 3309 3723 3971 4006
Size: 745 13 872 1306 148 414 248 35
Cuts at: 4006 225
Size: 2512
Fragments arranged by size:
2512 1306 872 745 414 248 148 35
13
BanII G_rGCy'C
Cuts at: 392 469 488 1918 2488 3307 4337 392
Size: 77 19 1430 570 819 1030 2348
Fragments arranged by size:
2348 1430 1030 819 570 77 19
BbeI G_GCGC'C
Cuts at: 3165 3727 3975 3165
Size: 562 248 5483
Fragments arranged by size:
5483 562 248
BbsI GAAGACnn'nnnn_
Cuts at: 463 1544 1570 1822 2045 3138 463
Size: 1081 26 252 223 1093 3618
Fragments arranged by size:
3618 1093 1081 252 223 26
BciVI GTATCCnnnnn_n'
Cuts at: 3262 4191 4952 6293 3262
Size: 929 761 1341 3262
Fragments arranged by size:
3262 1341 929 761
BclI T'GATC_A
Cuts at: 3813 3813
Size: 6293
BglII A'GATC_T
Cuts at: 1007 1509 3808 5845 1007
Size: 502 2299 2037 1455
Fragments arranged by size:
2299 2037 1455 502
BlpI GC'TnA_GC
Cuts at: 1273 3405 1273
Size: 2132 4161
Bme1580I G_kGCm'C
Cuts at: 392 587 2506 3918 4011 5067 392
Size: 195 1919 1412 93 1056 1618
Fragments arranged by size:
1919 1618 1412 1056 195 93
BmrI ACTGGGnnnn_n'
Cuts at: 2468 3505 3586 3921 6065 2468
Size: 1037 81 335 2144 2696
Fragments arranged by size:
2696 2144 1037 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4660 4660
Size: 6293
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 1080 2472 2592 2692 184
Size: 896 1392 120 100 3785
Fragments arranged by size:
3785 1392 896 120 100
Bpu10I CC'TnA_GC
Cuts at: 3029 3029
Size: 6293
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 979 2284 2924 3469 4348 4840 5138 979
Size: 1305 640 545 879 492 298 2134
Fragments arranged by size:
2134 1305 879 640 545 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 891 1303 5997 891
Size: 412 4694 1187
Fragments arranged by size:
4694 1187 412
BsaAI yAC'GTr
Cuts at: 3646 3646
Size: 6293
BsaBI GATnn'nnATC
Cuts at: 6 3807 5844 6
Size: 3801 2037 455
Fragments arranged by size:
3801 2037 455
BsaHI Gr'CG_yC
Cuts at: 1183 3162 3724 3972 4674 6170 1183
Size: 1979 562 248 702 1496 1306
Fragments arranged by size:
1979 1496 1306 702 562 248
BsaWI w'CCGG_w
Cuts at: 488 3482 4003 4681 4955 5102 5513 488
Size: 2994 521 678 274 147 411 1268
Fragments arranged by size:
2994 1268 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1369 1399 1369
Size: 30 6263
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 346 2224 2602 2605 2608 2611 2997 3000 3014
Size: 1878 378 3 3 3 386 3 14
Cuts at: 3014 346
Size: 3625
Fragments arranged by size:
3625 1878 386 378 14 3 3 3
3
BseYI C'CCAG_C
Cuts at: 1599 2352 2362 2499 2528 2858 3155 3718 4476
Size: 753 10 137 29 330 297 563 758
Cuts at: 4476 5053 5549 1599
Size: 577 496 2343
Fragments arranged by size:
2343 758 753 577 563 496 330 297
137 29 10
BsiEI CG_ry'CG
Cuts at: 104 189 3249 3835 3881 5089 104
Size: 85 3060 586 46 1208 1308
Fragments arranged by size:
3060 1308 1208 586 85 46
BsiHKAI G_wGCw'C
Cuts at: 488 1279 2488 3307 4085 4275 5067 488
Size: 791 1209 819 778 190 792 1714
Fragments arranged by size:
1714 1209 819 792 791 778 190
BsmI GAATG_Cn'
Cuts at: 2285 2285
Size: 6293
Bsp1286I G_dGCh'C
Cuts at: 392 469 488 587 1279 1918 2488 2506 3307
Size: 77 19 99 692 639 570 18 801
Cuts at: 3307 3918 4011 4085 4275 4337 5067 392
Size: 611 93 74 190 62 730 1618
Fragments arranged by size:
1618 801 730 692 639 611 570 190
99 93 77 74 62 19 18
BspEI T'CCGG_A
Cuts at: 488 5513 488
Size: 5025 1268
BspHI T'CATG_A
Cuts at: 272 923 5469 272
Size: 651 4546 1096
Fragments arranged by size:
4546 1096 651
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2304 2414 3343 3557 3859 4240 2304
Size: 110 929 214 302 381 4357
Fragments arranged by size:
4357 929 381 302 214 110
BsrBI CCG'CTC
Cuts at: 50 3082 4585 4639 6123 6288 50
Size: 3032 1503 54 1484 165 55
Fragments arranged by size:
3032 1503 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4205 4205
Size: 6293
BsrFI r'CCGG_y
Cuts at: 184 228 245 1603 2427 4291 4472 4681 184
Size: 44 17 1358 824 1864 181 209 1796
Fragments arranged by size:
1864 1796 1358 824 209 181 44 17
BsrGI T'GTAC_A
Cuts at: 906 906
Size: 6293
BssSI C'ACGA_G
Cuts at: 4564 4922 4564
Size: 358 5935
BstAPI GCAn_nnn'nTGC
Cuts at: 2393 2845 3670 2393
Size: 452 825 5016
Fragments arranged by size:
5016 825 452
BstBI TT'CG_AA
Cuts at: 846 4654 846
Size: 3808 2485
BstXI CCAn_nnnn'nTGG
Cuts at: 2570 3132 3271 2570
Size: 562 139 5592
Fragments arranged by size:
5592 562 139
BstYI r'GATC_y
Cuts at: 1 415 1007 1166 1472 1509 1523 1592 3318
Size: 414 592 159 306 37 14 69 1726
Cuts at: 3318 3785 3808 4143 4389 5401 5487 5499 5845
Size: 467 23 335 246 1012 86 12 346
Cuts at: 5845 1
Size: 449
Fragments arranged by size:
1726 1012 592 467 449 414 346 335
306 246 159 86 69 37 23 14
12
BtgI C'CryG_G
Cuts at: 411 551 1398 2740 411
Size: 140 847 1342 3964
Fragments arranged by size:
3964 1342 847 140
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4243 4424 4587 4243
Size: 181 163 5949
Fragments arranged by size:
5949 181 163
BtsI GCAGTG_nn'
Cuts at: 360 1663 2484 2499 3536 360
Size: 1303 821 15 1037 3117
Fragments arranged by size:
3117 1303 1037 821 15
DraI TTT'AAA
Cuts at: 3292 3292
Size: 6293
DraIII CAC_nnn'GTG
Cuts at: 1163 1163
Size: 6293
DrdI GACnn_nn'nnGTC
Cuts at: 3999 4857 3999
Size: 858 5435
EaeI y'GGCC_r
Cuts at: 182 186 874 1514 1767 2399 2560 3224 3246
Size: 4 688 640 253 632 161 664 22
Cuts at: 3246 3878 4052 4443 4470 5815 182
Size: 632 174 391 27 1345 660
Fragments arranged by size:
1345 688 664 660 640 632 632 391
253 174 161 27 22 4
EagI C'GGCC_G
Cuts at: 186 3246 3878 186
Size: 3060 632 2601
Fragments arranged by size:
3060 2601 632
EarI CTCTTCn'nnn_
Cuts at: 323 757 1294 1720 1954 4316 4526 323
Size: 434 537 426 234 2362 210 2090
Fragments arranged by size:
2362 2090 537 434 426 234 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1012 1516 4501 4811 4957 6103 1012
Size: 504 2985 310 146 1146 1202
Fragments arranged by size:
2985 1202 1146 504 310 146
EcoICRI GAG'CTC
Cuts at: 486 2486 3305 486
Size: 2000 819 3474
Fragments arranged by size:
3474 2000 819
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 547 561 619 1080 1275 1625 1720 1834
Size: 363 14 58 461 195 350 95 114
Cuts at: 1834 2472 2592 2692 2935 3152 4118 4550 5297
Size: 638 120 100 243 217 966 432 747
Cuts at: 5297 5565 184
Size: 268 912
Fragments arranged by size:
966 912 747 638 461 432 363 350
268 243 217 195 120 114 100 95
58 14
EcoNI CCTnn'n_nnAGG
Cuts at: 601 1437 2344 601
Size: 836 907 4550
Fragments arranged by size:
4550 907 836
EcoO109I rG'GnC_Cy
Cuts at: 389 1759 2323 2349 2804 3045 3219 3432 389
Size: 1370 564 26 455 241 174 213 3250
Fragments arranged by size:
3250 1370 564 455 241 213 174 26
EcoRI G'AATT_C
Cuts at: 556 796 2724 3297 556
Size: 240 1928 573 3552
Fragments arranged by size:
3552 1928 573 240
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1731 2275 4312 1731
Size: 544 2037 3712
Fragments arranged by size:
3712 2037 544
FseI GG_CCGG'CC
Cuts at: 188 188
Size: 6293
FspI TGC'GCA
Cuts at: 4074 4074
Size: 6293
HaeII r_GCGC'y
Cuts at: 2279 3165 3727 3975 4997 5976 2279
Size: 886 562 248 1022 979 2596
Fragments arranged by size:
2596 1022 979 886 562 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1767 1799 1867 1899 2754 2786 3034 3066 4135
Size: 32 68 32 855 32 248 32 1069
Cuts at: 4135 4165 4167 4197 1767
Size: 30 2 30 3863
Fragments arranged by size:
3863 1069 855 248 68 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 3332 3332
Size: 6293
HindIII A'AGCT_T
Cuts at: 727 2257 727
Size: 1530 4763
KasI G'GCGC_C
Cuts at: 3161 3723 3971 3161
Size: 562 248 5483
Fragments arranged by size:
5483 562 248
KpnI G_GTAC'C
Cuts at: 987 3313 987
Size: 2326 3967
MluI A'CGCG_T
Cuts at: 141 5726 141
Size: 5585 708
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1572 4964 5148 1572
Size: 3392 184 2717
Fragments arranged by size:
3392 2717 184
MscI TGG'CCA
Cuts at: 876 1516 1769 2401 3226 4054 876
Size: 640 253 632 825 828 3115
Fragments arranged by size:
3115 828 825 640 632 253
MslI CAynn'nnrTG
Cuts at: 928 1237 1466 1642 3130 3269 4282 4409 928
Size: 309 229 176 1488 139 1013 127 2812
Fragments arranged by size:
2812 1488 1013 309 229 176 139 127
MspA1I CmG'CkG
Cuts at: 758 2845 3159 3404 3718 4078 5091 5336 758
Size: 2087 314 245 314 360 1013 245 1715
Fragments arranged by size:
2087 1715 1013 360 314 314 245 245
NaeI GCC'GGC
Cuts at: 186 247 1605 4474 186
Size: 61 1358 2869 2005
Fragments arranged by size:
2869 2005 1358 61
NarI GG'CG_CC
Cuts at: 3162 3724 3972 3162
Size: 562 248 5483
Fragments arranged by size:
5483 562 248
NcoI C'CATG_G
Cuts at: 1398 2740 1398
Size: 1342 4951
NgoMIV G'CCGG_C
Cuts at: 184 245 1603 4472 184
Size: 61 1358 2869 2005
Fragments arranged by size:
2869 2005 1358 61
NspI r_CATG'y
Cuts at: 291 2391 3346 3846 4753 291
Size: 2100 955 500 907 1831
Fragments arranged by size:
2100 1831 955 907 500
PasI CC'CwG_GG
Cuts at: 2539 2539
Size: 6293
PciI A'CATG_T
Cuts at: 287 4749 287
Size: 4462 1831
PflMI CCAn_nnn'nTGG
Cuts at: 1163 1319 1163
Size: 156 6137
PfoI T'CCnGG_A
Cuts at: 560 1340 1475 560
Size: 780 135 5378
Fragments arranged by size:
5378 780 135
PmeI GTTT'AAAC
Cuts at: 3292 3292
Size: 6293
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5432 5464 5432
Size: 32 6261
PpuMI rG'GwC_Cy
Cuts at: 2323 2804 2323
Size: 481 5812
PspOMI G'GGCC_C
Cuts at: 388 388
Size: 6293
PstI C_TGCA'G
Cuts at: 1589 2075 2411 2447 2841 3340 4025 1589
Size: 486 336 36 394 499 685 3857
Fragments arranged by size:
3857 685 499 486 394 336 36
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6293
PvuII CAG'CTG
Cuts at: 2845 3718 4078 2845
Size: 873 360 5060
Fragments arranged by size:
5060 873 360
RsrII CG'GwC_CG
Cuts at: 4488 4488
Size: 6293
SacI G_AGCT'C
Cuts at: 488 2488 3307 488
Size: 2000 819 3474
Fragments arranged by size:
3474 2000 819
SalI G'TCGA_C
Cuts at: 3330 3330
Size: 6293
SanDI GG'GwC_CC
Cuts at: 2804 2804
Size: 6293
SapI GCTCTTCn'nnn_
Cuts at: 4316 4526 4316
Size: 210 6083
SbfI CC_TGCA'GG
Cuts at: 2447 3340 2447
Size: 893 5400
ScaI AGT'ACT
Cuts at: 916 5810 916
Size: 4894 1399
SexAI A'CCwGG_T
Cuts at: 1100 1391 1100
Size: 291 6002
SfcI C'TryA_G
Cuts at: 33 1585 2071 2407 2443 2837 2869 3336 4021
Size: 1552 486 336 36 394 32 467 685
Cuts at: 4021 5014 5205 33
Size: 993 191 1121
Fragments arranged by size:
1552 1121 993 685 486 467 394 336
191 36 32
SfoI GGC'GCC
Cuts at: 3163 3725 3973 3163
Size: 562 248 5483
Fragments arranged by size:
5483 562 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6293
SmaI CCC'GGG
Cuts at: 2809 3315 2809
Size: 506 5787
SmlI C'TyrA_G
Cuts at: 994 2299 2939 3448 4363 4855 5117 994
Size: 1305 640 509 915 492 262 2170
Fragments arranged by size:
2170 1305 915 640 509 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6293
SphI G_CATG'C
Cuts at: 2391 3346 3846 2391
Size: 955 500 4838
Fragments arranged by size:
4838 955 500
StuI AGG'CCT
Cuts at: 1697 2449 1697
Size: 752 5541
StyI C'CwwG_G
Cuts at: 1398 1706 1754 2740 3427 3781 1398
Size: 308 48 986 687 354 3910
Fragments arranged by size:
3910 986 687 354 308 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3876 4009 4468 4677 3876
Size: 133 459 209 5492
Fragments arranged by size:
5492 459 209 133
TatI w'GTAC_w
Cuts at: 906 914 990 3074 5808 906
Size: 8 76 2084 2734 1391
Fragments arranged by size:
2734 2084 1391 76 8
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1369 1401 2256 2288 3069 3101 5810 5842 1369
Size: 32 855 32 781 32 2709 32 1820
Fragments arranged by size:
2709 1820 855 781 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4090 4090
Size: 6293
XbaI T'CTAG_A
Cuts at: 3324 3324
Size: 6293
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1555 2313 2496 3219 3788 1555
Size: 758 183 723 569 4060
Fragments arranged by size:
4060 758 723 569 183
XmaI C'CCGG_G
Cuts at: 2807 3313 2807
Size: 506 5787
XmnI GAAnn'nnTTC
Cuts at: 521 521
Size: 6293
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI AvrII BaeI
BbvCI BcgI BfrBI BglI BmgBI BmtI BsgI BsiWI
BsmBI BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoRV
FspAI HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PmlI PshAI PsiI PspXI PsrI SacII SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI ZraI