(Circular) (Six-base) MAPSORT of: pF1KA1113.seq Check: 9471 from: 1 to: 6494 pF1KA1113 6494 bp With 182 enzymes: SgfI * September 18, 2009 16:59 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 487 1320 487 Size: 833 5661 AccI GT'mk_AC Cuts at: 3532 3532 Size: 6494 Acc65I G'GTAC_C Cuts at: 3510 3510 Size: 6494 AclI AA'CG_TT Cuts at: 6334 6334 Size: 6494 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 737 2471 3121 4319 4751 5498 5766 737 Size: 1734 650 1198 432 747 268 1465 Fragments arranged by size: 1734 1465 1198 747 650 432 268 AflIII A'CryG_T Cuts at: 826 976 991 3846 4950 5927 826 Size: 150 15 2855 1104 977 1393 Fragments arranged by size: 2855 1393 1104 977 150 15 AgeI A'CCGG_T Cuts at: 4882 4882 Size: 6494 AleI CACnn'nnGTG Cuts at: 226 2323 226 Size: 2097 4397 AlwNI CAG_nnn'CTG Cuts at: 901 1558 1732 3799 5366 901 Size: 657 174 2067 1567 2029 Fragments arranged by size: 2067 2029 1567 657 174 ApaI G_GGCC'C Cuts at: 194 293 194 Size: 99 6395 ApaLI G'TGCA_C Cuts at: 5264 5264 Size: 6494 ApoI r'AATT_y Cuts at: 853 3049 3498 5847 6103 853 Size: 2196 449 2349 256 1244 Fragments arranged by size: 2349 2196 1244 449 256 AseI AT'TA_AT Cuts at: 20 1226 3269 20 Size: 1206 2043 3245 Fragments arranged by size: 3245 2043 1206 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 6494 AvaI C'yCGr_G Cuts at: 321 571 603 3514 321 Size: 250 32 2911 3301 Fragments arranged by size: 3301 2911 250 32 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 1015 1048 1015 Size: 33 6461 BamHI G'GATC_C Cuts at: 3519 3519 Size: 6494 BanI G'GyrC_C Cuts at: 647 3510 3924 4172 4207 647 Size: 2863 414 248 35 2934 Fragments arranged by size: 2934 2863 414 248 35 BanII G_rGCy'C Cuts at: 194 293 328 527 2041 2669 3508 4538 194 Size: 99 35 199 1514 628 839 1030 2150 Fragments arranged by size: 2150 1514 1030 839 628 199 99 35 BbeI G_GCGC'C Cuts at: 3928 4176 3928 Size: 248 6246 BbsI GAAGACnn'nnnn_ Cuts at: 535 776 2401 2774 3004 3434 535 Size: 241 1625 373 230 430 3595 Fragments arranged by size: 3595 1625 430 373 241 230 BbvCI CC'TCA_GC Cuts at: 272 272 Size: 6494 BciVI GTATCCnnnnn_n' Cuts at: 1904 4392 5153 6494 1904 Size: 2488 761 1341 1904 Fragments arranged by size: 2488 1904 1341 761 BclI T'GATC_A Cuts at: 872 1814 2418 4014 872 Size: 942 604 1596 3352 Fragments arranged by size: 3352 1596 942 604 BfrBI ATG'CAT Cuts at: 2596 2596 Size: 6494 BglII A'GATC_T Cuts at: 2799 3344 4009 6046 2799 Size: 545 665 2037 3247 Fragments arranged by size: 3247 2037 665 545 BlpI GC'TnA_GC Cuts at: 589 2668 3606 589 Size: 2079 938 3477 Fragments arranged by size: 3477 2079 938 Bme1580I G_kGCm'C Cuts at: 194 293 367 599 652 4119 4212 5268 194 Size: 99 74 232 53 3467 93 1056 1420 Fragments arranged by size: 3467 1420 1056 232 99 93 74 53 BmrI ACTGGGnnnn_n' Cuts at: 646 3706 3787 4122 6266 646 Size: 3060 81 335 2144 874 Fragments arranged by size: 3060 2144 874 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1901 2818 3366 4861 1901 Size: 917 548 1495 3534 Fragments arranged by size: 3534 1495 917 548 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 386 389 392 1580 1639 2252 386 Size: 3 3 1188 59 613 4628 Fragments arranged by size: 4628 1188 613 59 3 3 Bpu10I CC'TnA_GC Cuts at: 272 3296 272 Size: 3024 3470 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1120 2169 3670 4549 5041 5339 1120 Size: 1049 1501 879 492 298 2275 Fragments arranged by size: 2275 1501 1049 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 2322 3465 6198 2322 Size: 1143 2733 2618 Fragments arranged by size: 2733 2618 1143 BsaAI yAC'GTr Cuts at: 3847 3847 Size: 6494 BsaBI GATnn'nnATC Cuts at: 6 4008 6045 6 Size: 4002 2037 455 Fragments arranged by size: 4002 2037 455 BsaHI Gr'CG_yC Cuts at: 394 3925 4173 4875 6371 394 Size: 3531 248 702 1496 517 Fragments arranged by size: 3531 1496 702 517 248 BsaWI w'CCGG_w Cuts at: 3683 4204 4882 5156 5303 5714 3683 Size: 521 678 274 147 411 4463 Fragments arranged by size: 4463 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 384 414 384 Size: 30 6464 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 253 256 259 262 309 1796 2526 2747 2810 Size: 3 3 3 47 1487 730 221 63 Cuts at: 2810 253 Size: 3937 Fragments arranged by size: 3937 1487 730 221 63 47 3 3 3 BseYI C'CCAG_C Cuts at: 3919 4677 5254 5750 3919 Size: 758 577 496 4663 Fragments arranged by size: 4663 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 803 1159 803 Size: 356 6138 BsiEI CG_ry'CG Cuts at: 104 287 4036 4082 5290 104 Size: 183 3749 46 1208 1308 Fragments arranged by size: 3749 1308 1208 183 46 BsiHKAI G_wGCw'C Cuts at: 3508 4286 4476 5268 3508 Size: 778 190 792 4734 Fragments arranged by size: 4734 792 778 190 BsmI GAATG_Cn' Cuts at: 673 1896 673 Size: 1223 5271 Bsp1286I G_dGCh'C Cuts at: 194 293 328 367 527 599 652 2041 2669 Size: 99 35 39 160 72 53 1389 628 Cuts at: 2669 3508 4119 4212 4286 4476 4538 5268 194 Size: 839 611 93 74 190 62 730 1420 Fragments arranged by size: 1420 1389 839 730 628 611 190 160 99 93 74 72 62 53 39 35 BspEI T'CCGG_A Cuts at: 5714 5714 Size: 6494 BspHI T'CATG_A Cuts at: 5670 5670 Size: 6494 BspMI ACCTGCnnnn'nnnn_ Cuts at: 487 1320 2579 2640 3544 3758 4060 4441 487 Size: 833 1259 61 904 214 302 381 2540 Fragments arranged by size: 2540 1259 904 833 381 302 214 61 BsrBI CCG'CTC Cuts at: 50 143 424 4786 4840 6324 6489 50 Size: 93 281 4362 54 1484 165 55 Fragments arranged by size: 4362 1484 281 165 93 55 54 BsrDI GCAATG_nn' Cuts at: 4406 4406 Size: 6494 BsrFI r'CCGG_y Cuts at: 168 265 396 509 4492 4673 4882 168 Size: 97 131 113 3983 181 209 1780 Fragments arranged by size: 3983 1780 209 181 131 113 97 BsrGI T'GTAC_A Cuts at: 939 939 Size: 6494 BssHII G'CGCG_C Cuts at: 164 164 Size: 6494 BssSI C'ACGA_G Cuts at: 4765 5123 4765 Size: 358 6136 BstAPI GCAn_nnn'nTGC Cuts at: 501 1830 3871 501 Size: 1329 2041 3124 Fragments arranged by size: 3124 2041 1329 BstBI TT'CG_AA Cuts at: 2700 4855 2700 Size: 2155 4339 BstXI CCAn_nnnn'nTGG Cuts at: 634 2169 634 Size: 1535 4959 BstYI r'GATC_y Cuts at: 1 2799 3344 3519 3986 4009 4344 4590 5602 Size: 2798 545 175 467 23 335 246 1012 Cuts at: 5602 5688 5700 6046 1 Size: 86 12 346 449 Fragments arranged by size: 2798 1012 545 467 449 346 335 246 175 86 23 12 BtgI C'CryG_G Cuts at: 107 224 311 2987 107 Size: 117 87 2676 3614 Fragments arranged by size: 3614 2676 117 87 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2126 4444 4625 4788 2126 Size: 2318 181 163 3832 Fragments arranged by size: 3832 2318 181 163 BtsI GCAGTG_nn' Cuts at: 3737 3737 Size: 6494 DraI TTT'AAA Cuts at: 3493 3493 Size: 6494 DraIII CAC_nnn'GTG Cuts at: 1447 1447 Size: 6494 DrdI GACnn_nn'nnGTC Cuts at: 4200 5058 4200 Size: 858 5636 EaeI y'GGCC_r Cuts at: 284 308 1199 3182 4079 4253 4644 4671 6016 Size: 24 891 1983 897 174 391 27 1345 Cuts at: 6016 284 Size: 762 Fragments arranged by size: 1983 1345 897 891 762 391 174 27 24 EagI C'GGCC_G Cuts at: 284 4079 284 Size: 3795 2699 EarI CTCTTCn'nnn_ Cuts at: 3386 4517 4727 3386 Size: 1131 210 5153 Fragments arranged by size: 5153 1131 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 127 223 535 4702 5012 5158 6304 127 Size: 96 312 4167 310 146 1146 317 Fragments arranged by size: 4167 1146 317 312 310 146 96 EcoICRI GAG'CTC Cuts at: 3506 3506 Size: 6494 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 386 389 392 737 1580 1639 2252 2471 3121 Size: 3 3 345 843 59 613 219 650 Cuts at: 3121 4319 4751 5498 5766 386 Size: 1198 432 747 268 1114 Fragments arranged by size: 1198 1114 843 747 650 613 432 345 268 219 59 3 3 EcoNI CCTnn'n_nnAGG Cuts at: 2267 2267 Size: 6494 EcoO109I rG'GnC_Cy Cuts at: 430 2074 2272 3359 3633 430 Size: 1644 198 1087 274 3291 Fragments arranged by size: 3291 1644 1087 274 198 EcoRI G'AATT_C Cuts at: 3049 3498 3049 Size: 449 6045 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4513 4513 Size: 6494 FspI TGC'GCA Cuts at: 2972 4275 2972 Size: 1303 5191 HaeII r_GCGC'y Cuts at: 272 585 3928 4176 5198 6177 272 Size: 313 3343 248 1022 979 589 Fragments arranged by size: 3343 1022 979 589 313 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 384 416 727 759 2167 2199 2541 2573 4336 Size: 32 311 32 1408 32 342 32 1763 Cuts at: 4336 4366 4368 4398 384 Size: 30 2 30 2480 Fragments arranged by size: 2480 1763 1408 342 311 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 919 3533 919 Size: 2614 3880 HindIII A'AGCT_T Cuts at: 1049 3133 1049 Size: 2084 4410 KasI G'GCGC_C Cuts at: 3924 4172 3924 Size: 248 6246 KpnI G_GTAC'C Cuts at: 3514 3514 Size: 6494 MfeI C'AATT_G Cuts at: 1073 1364 1073 Size: 291 6203 MluI A'CGCG_T Cuts at: 5927 5927 Size: 6494 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 885 2150 2878 5165 5349 885 Size: 1265 728 2287 184 2030 Fragments arranged by size: 2287 2030 1265 728 184 MscI TGG'CCA Cuts at: 1201 4255 1201 Size: 3054 3440 MslI CAynn'nnrTG Cuts at: 226 2323 4483 4610 226 Size: 2097 2160 127 2110 Fragments arranged by size: 2160 2110 2097 127 MspA1I CmG'CkG Cuts at: 158 226 313 1925 2366 3605 3919 4279 5292 Size: 68 87 1612 441 1239 314 360 1013 Cuts at: 5292 5537 158 Size: 245 1115 Fragments arranged by size: 1612 1239 1115 1013 441 360 314 245 87 68 NaeI GCC'GGC Cuts at: 267 398 511 4675 267 Size: 131 113 4164 2086 Fragments arranged by size: 4164 2086 131 113 NarI GG'CG_CC Cuts at: 3925 4173 3925 Size: 248 6246 NcoI C'CATG_G Cuts at: 107 107 Size: 6494 NgoMIV G'CCGG_C Cuts at: 265 396 509 4673 265 Size: 131 113 4164 2086 Fragments arranged by size: 4164 2086 131 113 NsiI A_TGCA'T Cuts at: 2598 2598 Size: 6494 NspI r_CATG'y Cuts at: 830 980 995 1342 1665 1987 2526 3547 4047 Size: 150 15 347 323 322 539 1021 500 Cuts at: 4047 4954 830 Size: 907 2370 Fragments arranged by size: 2370 1021 907 539 500 347 323 322 150 15 PasI CC'CwG_GG Cuts at: 1978 1978 Size: 6494 PciI A'CATG_T Cuts at: 826 976 991 4950 826 Size: 150 15 3959 2370 Fragments arranged by size: 3959 2370 150 15 PflMI CCAn_nnn'nTGG Cuts at: 1502 1558 1502 Size: 56 6438 PfoI T'CCnGG_A Cuts at: 450 470 2289 3053 3167 450 Size: 20 1819 764 114 3777 Fragments arranged by size: 3777 1819 764 114 20 PmeI GTTT'AAAC Cuts at: 3493 3493 Size: 6494 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5633 5665 5633 Size: 32 6462 PpuMI rG'GwC_Cy Cuts at: 430 2074 2272 3359 430 Size: 1644 198 1087 3565 Fragments arranged by size: 3565 1644 1087 198 PsiI TTA'TAA Cuts at: 3097 3097 Size: 6494 PspOMI G'GGCC_C Cuts at: 190 289 190 Size: 99 6395 PstI C_TGCA'G Cuts at: 2637 3541 4226 2637 Size: 904 685 4905 Fragments arranged by size: 4905 904 685 PvuI CG_AT'CG Cuts at: 104 104 Size: 6494 PvuII CAG'CTG Cuts at: 2366 3919 4279 2366 Size: 1553 360 4581 Fragments arranged by size: 4581 1553 360 RsrII CG'GwC_CG Cuts at: 4689 4689 Size: 6494 SacI G_AGCT'C Cuts at: 3508 3508 Size: 6494 SacII CC_GC'GG Cuts at: 227 314 227 Size: 87 6407 SalI G'TCGA_C Cuts at: 3531 3531 Size: 6494 SanDI GG'GwC_CC Cuts at: 430 430 Size: 6494 SapI GCTCTTCn'nnn_ Cuts at: 4517 4727 4517 Size: 210 6284 SbfI CC_TGCA'GG Cuts at: 3541 3541 Size: 6494 ScaI AGT'ACT Cuts at: 2340 2349 6011 2340 Size: 9 3662 2823 Fragments arranged by size: 3662 2823 9 SfcI C'TryA_G Cuts at: 33 1294 2045 2633 3537 4222 5215 5406 33 Size: 1261 751 588 904 685 993 191 1121 Fragments arranged by size: 1261 1121 993 904 751 685 588 191 SfoI GGC'GCC Cuts at: 3926 4174 3926 Size: 248 6246 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 6494 SmaI CCC'GGG Cuts at: 605 3516 605 Size: 2911 3583 SmlI C'TyrA_G Cuts at: 1099 2184 3649 4564 5056 5318 1099 Size: 1085 1465 915 492 262 2275 Fragments arranged by size: 2275 1465 1085 915 492 262 SpeI A'CTAG_T Cuts at: 67 760 67 Size: 693 5801 SphI G_CATG'C Cuts at: 1987 3547 4047 1987 Size: 1560 500 4434 Fragments arranged by size: 4434 1560 500 SspI AAT'ATT Cuts at: 1936 1936 Size: 6494 StuI AGG'CCT Cuts at: 1319 1319 Size: 6494 StyI C'CwwG_G Cuts at: 107 3628 3982 107 Size: 3521 354 2619 Fragments arranged by size: 3521 2619 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 2175 4077 4210 4669 4878 2175 Size: 1902 133 459 209 3791 Fragments arranged by size: 3791 1902 459 209 133 TatI w'GTAC_w Cuts at: 757 939 2338 2347 3320 3477 6009 757 Size: 182 1399 9 973 157 2532 1242 Fragments arranged by size: 2532 1399 1242 973 182 157 9 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2244 2276 6011 6043 2244 Size: 32 3735 32 2695 Fragments arranged by size: 3735 2695 32 32 Tth111I GACn'n_nGTC Cuts at: 4291 4291 Size: 6494 XbaI T'CTAG_A Cuts at: 2577 3525 2577 Size: 948 5546 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2768 2879 3989 2768 Size: 111 1110 5273 Fragments arranged by size: 5273 1110 111 XmaI C'CCGG_G Cuts at: 603 3514 603 Size: 2911 3583 XmnI GAAnn'nnTTC Cuts at: 964 1052 2135 3011 964 Size: 88 1083 876 4447 Fragments arranged by size: 4447 1083 876 88 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AatII AfeI AflII AhdI AlfI AloI AscI AvrII BcgI BglI BmgBI BmtI BsiWI BsmBI BstEII BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI HpaI NdeI NheI NotI NruI PacI PmlI PshAI PspXI PsrI SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI ZraI