Restriction map for
pF1KA1114
(Circular) (Six-base) MAPSORT of: pF1KA1114.seq Check: 3241 from: 1 to: 7406
pF1KA1114 7406 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 366 366
Size: 7406
AccI GT'mk_AC
Cuts at: 4444 4444
Size: 7406
Acc65I G'GTAC_C
Cuts at: 3838 4422 3838
Size: 584 6822
AclI AA'CG_TT
Cuts at: 7246 7246
Size: 7406
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 482 625 2099 2259 2289 2343 2397 2451 2727
Size: 143 1474 160 30 54 54 54 276
Cuts at: 2727 3327 3357 3777 3897 4161 4191 4248 5231
Size: 600 30 420 120 264 30 57 983
Cuts at: 5231 5663 6410 6678 482
Size: 432 747 268 1210
Fragments arranged by size:
1474 1210 983 747 600 432 420 276
268 264 160 143 120 57 54 54
54 30 30 30
AfeI AGC'GCT
Cuts at: 3846 3846
Size: 7406
AflIII A'CryG_T
Cuts at: 4758 5862 6839 4758
Size: 1104 977 5325
Fragments arranged by size:
5325 1104 977
AgeI A'CCGG_T
Cuts at: 3691 5794 3691
Size: 2103 5303
AlwNI CAG_nnn'CTG
Cuts at: 505 1272 2798 2858 2978 3128 3248 3548 3578
Size: 767 1526 60 120 150 120 300 30
Cuts at: 3578 3638 4288 4711 6278 505
Size: 60 650 423 1567 1633
Fragments arranged by size:
1633 1567 1526 767 650 423 300 150
120 120 60 60 30
ApaI G_GGCC'C
Cuts at: 157 1036 1200 2138 157
Size: 879 164 938 5425
Fragments arranged by size:
5425 938 879 164
ApaLI G'TGCA_C
Cuts at: 2336 2498 2720 3170 3290 3380 6176 2336
Size: 162 222 450 120 90 2796 3566
Fragments arranged by size:
3566 2796 450 222 162 120 90
ApoI r'AATT_y
Cuts at: 1038 4410 6759 7015 1038
Size: 3372 2349 256 1429
Fragments arranged by size:
3372 2349 1429 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7406
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7406
AvaI C'yCGr_G
Cuts at: 168 1157 1322 4426 168
Size: 989 165 3104 3148
Fragments arranged by size:
3148 3104 989 165
AvrII C'CTAG_G
Cuts at: 3774 3774
Size: 7406
BamHI G'GATC_C
Cuts at: 1391 4431 1391
Size: 3040 4366
BanI G'GyrC_C
Cuts at: 138 3148 3538 3778 3838 3868 3898 3928 3988
Size: 3010 390 240 60 30 30 30 60
Cuts at: 3988 4018 4378 4422 4836 5084 5119 138
Size: 30 360 44 414 248 35 2425
Fragments arranged by size:
3010 2425 414 390 360 248 240 60
60 44 35 30 30 30 30
BanII G_rGCy'C
Cuts at: 157 1036 1137 1200 1310 2103 2138 2241 3564
Size: 879 101 63 110 793 35 103 1323
Cuts at: 3564 4420 5450 157
Size: 856 1030 2113
Fragments arranged by size:
2113 1323 1030 879 856 793 110 103
101 63 35
BbeI G_GCGC'C
Cuts at: 4840 5088 4840
Size: 248 7158
BbsI GAAGACnn'nnnn_
Cuts at: 898 2733 898
Size: 1835 5571
BbvCI CC'TCA_GC
Cuts at: 1519 2072 2090 4045 1519
Size: 553 18 1955 4880
Fragments arranged by size:
4880 1955 553 18
BciVI GTATCCnnnnn_n'
Cuts at: 850 5304 6065 7406 850
Size: 4454 761 1341 850
Fragments arranged by size:
4454 1341 850 761
BclI T'GATC_A
Cuts at: 4926 4926
Size: 7406
BfrBI ATG'CAT
Cuts at: 243 243
Size: 7406
BglI GCCn_nnn'nGGC
Cuts at: 1153 1762 1153
Size: 609 6797
BglII A'GATC_T
Cuts at: 1052 4921 6958 1052
Size: 3869 2037 1500
Fragments arranged by size:
3869 2037 1500
BlpI GC'TnA_GC
Cuts at: 509 2755 4518 509
Size: 2246 1763 3397
Fragments arranged by size:
3397 2246 1763
Bme1580I G_kGCm'C
Cuts at: 157 1036 1200 1239 2138 2340 2502 2724 3174
Size: 879 164 39 899 202 162 222 450
Cuts at: 3174 3294 3384 3444 3901 3931 3991 5031 5124
Size: 120 90 60 457 30 60 1040 93
Cuts at: 5124 6180 157
Size: 1056 1383
Fragments arranged by size:
1383 1056 1040 899 879 457 450 222
202 164 162 120 93 90 60 60
39 30
BmrI ACTGGGnnnn_n'
Cuts at: 1687 2019 3904 3934 3994 4184 4618 4699 5034
Size: 332 1885 30 60 190 434 81 335
Cuts at: 5034 7178 1687
Size: 2144 1915
Fragments arranged by size:
2144 1915 1885 434 335 332 190 81
60 30
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2680 2800 5773 2680
Size: 120 2973 4313
Fragments arranged by size:
4313 2973 120
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 179 353 1324 1606 1897 2226 2331 2445 2805
Size: 174 971 282 291 329 105 114 360
Cuts at: 2805 179
Size: 4780
Fragments arranged by size:
4780 971 360 329 291 282 174 114
105
Bpu10I CC'TnA_GC
Cuts at: 902 1519 2072 2090 2198 3805 4045 902
Size: 617 553 18 108 1607 240 4263
Fragments arranged by size:
4263 1607 617 553 240 108 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1261 1954 4582 5461 5953 6251 1261
Size: 693 2628 879 492 298 2416
Fragments arranged by size:
2628 2416 879 693 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 205 777 1714 7110 205
Size: 572 937 5396 501
Fragments arranged by size:
5396 937 572 501
BsaAI yAC'GTr
Cuts at: 4759 4759
Size: 7406
BsaBI GATnn'nnATC
Cuts at: 6 1283 1498 4920 6957 6
Size: 1277 215 3422 2037 455
Fragments arranged by size:
3422 2037 1277 455 215
BsaHI Gr'CG_yC
Cuts at: 4837 5085 5787 7283 4837
Size: 248 702 1496 4960
Fragments arranged by size:
4960 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3691 4595 5116 5794 6068 6215 6626 3691
Size: 904 521 678 274 147 411 4471
Fragments arranged by size:
4471 904 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1579 1609 1579
Size: 30 7376
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1702 2297 1702
Size: 595 6811
BseYI C'CCAG_C
Cuts at: 375 1704 2131 2341 2395 2815 2995 3265 3475
Size: 1329 427 210 54 420 180 270 210
Cuts at: 3475 3505 3565 3679 4159 4831 5589 6166 6662
Size: 30 60 114 480 672 758 577 496
Cuts at: 6662 375
Size: 1119
Fragments arranged by size:
1329 1119 758 672 577 496 480 427
420 270 210 210 180 114 60 54
30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 338 1397 2008 2038 2862 338
Size: 1059 611 30 824 4882
Fragments arranged by size:
4882 1059 824 611 30
BsiEI CG_ry'CG
Cuts at: 104 4948 4994 6202 104
Size: 4844 46 1208 1308
Fragments arranged by size:
4844 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2340 2455 2502 2724 2737 3024 3056 3091 3174
Size: 115 47 222 13 287 32 35 83
Cuts at: 3174 3294 3384 3534 3564 4282 4342 4420 5198
Size: 120 90 150 30 718 60 78 778
Cuts at: 5198 5388 6180 2340
Size: 190 792 3566
Fragments arranged by size:
3566 792 778 718 287 222 190 150
120 115 90 83 78 60 47 35
32 30 13
BsmI GAATG_Cn'
Cuts at: 1815 1815
Size: 7406
Bsp1286I G_dGCh'C
Cuts at: 157 1036 1137 1200 1239 1310 2103 2138 2241
Size: 879 101 63 39 71 793 35 103
Cuts at: 2241 2340 2455 2502 2724 2737 3024 3056 3091
Size: 99 115 47 222 13 287 32 35
Cuts at: 3091 3174 3294 3384 3444 3534 3564 3901 3931
Size: 83 120 90 60 90 30 337 30
Cuts at: 3931 3991 4282 4342 4420 5031 5124 5198 5388
Size: 60 291 60 78 611 93 74 190
Cuts at: 5388 5450 6180 157
Size: 62 730 1383
Fragments arranged by size:
1383 879 793 730 611 337 291 287
222 190 120 115 103 101 99 93
90 90 83 78 74 71 63 62
60 60 60 47 39 35 35 32
30 30 13
BspEI T'CCGG_A
Cuts at: 6626 6626
Size: 7406
BspHI T'CATG_A
Cuts at: 6582 6582
Size: 7406
BspMI ACCTGCnnnn'nnnn_
Cuts at: 366 440 609 4456 4670 4972 5353 366
Size: 74 169 3847 214 302 381 2419
Fragments arranged by size:
3847 2419 381 302 214 169 74
BsrBI CCG'CTC
Cuts at: 50 5698 5752 7236 7401 50
Size: 5648 54 1484 165 55
Fragments arranged by size:
5648 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3714 5318 3714
Size: 1604 5802
BsrFI r'CCGG_y
Cuts at: 299 3691 5404 5585 5794 299
Size: 3392 1713 181 209 1911
Fragments arranged by size:
3392 1911 1713 209 181
BssSI C'ACGA_G
Cuts at: 1945 5677 6035 1945
Size: 3732 358 3316
Fragments arranged by size:
3732 3316 358
BstAPI GCAn_nnn'nTGC
Cuts at: 361 4783 361
Size: 4422 2984
BstBI TT'CG_AA
Cuts at: 5767 5767
Size: 7406
BstEII G'GTnAC_C
Cuts at: 477 477
Size: 7406
BstXI CCAn_nnnn'nTGG
Cuts at: 589 589
Size: 7406
BstYI r'GATC_y
Cuts at: 1 1052 1391 1493 4431 4898 4921 5256 5502
Size: 1051 339 102 2938 467 23 335 246
Cuts at: 5502 6514 6600 6612 6958 1
Size: 1012 86 12 346 449
Fragments arranged by size:
2938 1051 1012 467 449 346 339 335
246 102 86 23 12
BtgI C'CryG_G
Cuts at: 107 278 1396 2672 107
Size: 171 1118 1276 4841
Fragments arranged by size:
4841 1276 1118 171
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2204 2324 4070 5356 5537 5700 2204
Size: 120 1746 1286 181 163 3910
Fragments arranged by size:
3910 1746 1286 181 163 120
BtsI GCAGTG_nn'
Cuts at: 503 2039 2532 2724 3054 3174 3594 4128 4248
Size: 1536 493 192 330 120 420 534 120
Cuts at: 4248 4365 4649 503
Size: 117 284 3260
Fragments arranged by size:
3260 1536 534 493 420 330 284 192
120 120 117
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2937 2972 2937
Size: 35 7371
DraI TTT'AAA
Cuts at: 428 4405 428
Size: 3977 3429
DrdI GACnn_nn'nnGTC
Cuts at: 5112 5970 5112
Size: 858 6548
EaeI y'GGCC_r
Cuts at: 275 579 1430 4991 5165 5556 5583 6928 275
Size: 304 851 3561 174 391 27 1345 753
Fragments arranged by size:
3561 1345 851 753 391 304 174 27
EagI C'GGCC_G
Cuts at: 4991 4991
Size: 7406
EarI CTCTTCn'nnn_
Cuts at: 215 529 2174 2937 5429 5639 215
Size: 314 1645 763 2492 210 1982
Fragments arranged by size:
2492 1982 1645 763 314 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1404 3935 5614 5924 6070 7216 1404
Size: 2531 1679 310 146 1146 1594
Fragments arranged by size:
2531 1679 1594 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3562 4418 3562
Size: 856 6550
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 179 353 482 625 1324 1606 1897 2099 2226
Size: 174 129 143 699 282 291 202 127
Cuts at: 2226 2259 2289 2331 2343 2397 2445 2451 2727
Size: 33 30 42 12 54 48 6 276
Cuts at: 2727 2805 3327 3357 3777 3897 4161 4191 4248
Size: 78 522 30 420 120 264 30 57
Cuts at: 4248 5231 5663 6410 6678 179
Size: 983 432 747 268 907
Fragments arranged by size:
983 907 747 699 522 432 420 291
282 276 268 264 202 174 143 129
127 120 78 57 54 48 42 33
30 30 30 12 6
EcoO109I rG'GnC_Cy
Cuts at: 153 154 327 621 1032 1062 1196 1269 1319
Size: 1 173 294 411 30 134 73 50
Cuts at: 1319 1890 2135 4545 153
Size: 571 245 2410 3014
Fragments arranged by size:
3014 2410 571 411 294 245 173 134
73 50 30 1
EcoRI G'AATT_C
Cuts at: 4410 4410
Size: 7406
EcoRV GAT'ATC
Cuts at: 2160 2160
Size: 7406
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 630 5425 630
Size: 4795 2611
FspI TGC'GCA
Cuts at: 1786 5187 1786
Size: 3401 4005
HaeII r_GCGC'y
Cuts at: 3848 4840 5088 6110 7089 3848
Size: 992 248 1022 979 4165
Fragments arranged by size:
4165 1022 992 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 5248 5278 5280 5310 5248
Size: 30 2 30 7344
Fragments arranged by size:
7344 30 30 2
HincII GTy'rAC
Cuts at: 297 2271 4445 297
Size: 1974 2174 3258
Fragments arranged by size:
3258 2174 1974
HindIII A'AGCT_T
Cuts at: 719 719
Size: 7406
KasI G'GCGC_C
Cuts at: 4836 5084 4836
Size: 248 7158
KpnI G_GTAC'C
Cuts at: 3842 4426 3842
Size: 584 6822
MfeI C'AATT_G
Cuts at: 662 896 662
Size: 234 7172
MluI A'CGCG_T
Cuts at: 6839 6839
Size: 7406
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4154 6077 6261 4154
Size: 1923 184 5299
Fragments arranged by size:
5299 1923 184
MscI TGG'CCA
Cuts at: 277 581 1432 5167 277
Size: 304 851 3735 2516
Fragments arranged by size:
3735 2516 851 304
MslI CAynn'nnrTG
Cuts at: 587 1355 1738 5395 5522 587
Size: 768 383 3657 127 2471
Fragments arranged by size:
3657 2471 768 383 127
MspA1I CmG'CkG
Cuts at: 379 603 2059 3454 4211 4268 4517 4831 5191
Size: 224 1456 1395 757 57 249 314 360
Cuts at: 5191 6204 6449 379
Size: 1013 245 1336
Fragments arranged by size:
1456 1395 1336 1013 757 360 314 249
245 224 57
NaeI GCC'GGC
Cuts at: 5587 5587
Size: 7406
NarI GG'CG_CC
Cuts at: 4837 5085 4837
Size: 248 7158
NcoI C'CATG_G
Cuts at: 107 278 1396 107
Size: 171 1118 6117
Fragments arranged by size:
6117 1118 171
NgoMIV G'CCGG_C
Cuts at: 5585 5585
Size: 7406
NsiI A_TGCA'T
Cuts at: 245 245
Size: 7406
NspI r_CATG'y
Cuts at: 1518 1982 2556 4459 4959 5866 1518
Size: 464 574 1903 500 907 3058
Fragments arranged by size:
3058 1903 907 574 500 464
PasI CC'CwG_GG
Cuts at: 1804 1804
Size: 7406
PciI A'CATG_T
Cuts at: 5862 5862
Size: 7406
PflMI CCAn_nnn'nTGG
Cuts at: 1098 1272 2009 4225 4312 1098
Size: 174 737 2216 87 4192
Fragments arranged by size:
4192 2216 737 174 87
PmeI GTTT'AAAC
Cuts at: 4405 4405
Size: 7406
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6545 6577 6545
Size: 32 7374
PpuMI rG'GwC_Cy
Cuts at: 621 1319 1890 621
Size: 698 571 6137
Fragments arranged by size:
6137 698 571
PspOMI G'GGCC_C
Cuts at: 153 1032 1196 2134 153
Size: 879 164 938 5425
Fragments arranged by size:
5425 938 879 164
PstI C_TGCA'G
Cuts at: 1671 2058 4243 4453 5138 1671
Size: 387 2185 210 685 3939
Fragments arranged by size:
3939 2185 685 387 210
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7406
PvuII CAG'CTG
Cuts at: 379 603 2059 4831 5191 379
Size: 224 1456 2772 360 2594
Fragments arranged by size:
2772 2594 1456 360 224
RsrII CG'GwC_CG
Cuts at: 5601 5601
Size: 7406
SacI G_AGCT'C
Cuts at: 3564 4420 3564
Size: 856 6550
SalI G'TCGA_C
Cuts at: 4443 4443
Size: 7406
SanDI GG'GwC_CC
Cuts at: 1890 1890
Size: 7406
SapI GCTCTTCn'nnn_
Cuts at: 2937 5429 5639 2937
Size: 2492 210 4704
Fragments arranged by size:
4704 2492 210
SbfI CC_TGCA'GG
Cuts at: 4453 4453
Size: 7406
ScaI AGT'ACT
Cuts at: 3066 6923 3066
Size: 3857 3549
SfcI C'TryA_G
Cuts at: 33 1667 2054 4239 4449 5134 6127 6318 33
Size: 1634 387 2185 210 685 993 191 1121
Fragments arranged by size:
2185 1634 1121 993 685 387 210 191
SfoI GGC'GCC
Cuts at: 4838 5086 4838
Size: 248 7158
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7406
SmaI CCC'GGG
Cuts at: 170 1324 4428 170
Size: 1154 3104 3148
Fragments arranged by size:
3148 3104 1154
SmlI C'TyrA_G
Cuts at: 1240 1969 4561 5476 5968 6230 1240
Size: 729 2592 915 492 262 2416
Fragments arranged by size:
2592 2416 915 729 492 262
SpeI A'CTAG_T
Cuts at: 67 2515 3961 67
Size: 2448 1446 3512
Fragments arranged by size:
3512 2448 1446
SphI G_CATG'C
Cuts at: 1982 2556 4459 4959 1982
Size: 574 1903 500 4429
Fragments arranged by size:
4429 1903 574 500
SspI AAT'ATT
Cuts at: 651 1549 651
Size: 898 6508
StuI AGG'CCT
Cuts at: 976 1410 2203 4074 976
Size: 434 793 1871 4308
Fragments arranged by size:
4308 1871 793 434
StyI C'CwwG_G
Cuts at: 107 263 278 548 558 725 810 1007 1191
Size: 156 15 270 10 167 85 197 184
Cuts at: 1191 1396 1688 2003 3774 4540 4894 107
Size: 205 292 315 1771 766 354 2619
Fragments arranged by size:
2619 1771 766 354 315 292 270 205
197 184 167 156 85 15 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 808 823 1713 2355 2409 2739 4290 4350 4989
Size: 15 890 642 54 330 1551 60 639
Cuts at: 4989 5122 5581 5790 808
Size: 133 459 209 2424
Fragments arranged by size:
2424 1551 890 642 639 459 330 209
133 60 54 15
TatI w'GTAC_w
Cuts at: 1880 2691 3064 6921 1880
Size: 811 373 3857 2365
Fragments arranged by size:
3857 2365 811 373
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 122 154 6923 6955 122
Size: 32 6769 32 573
Fragments arranged by size:
6769 573 32 32
Tth111I GACn'n_nGTC
Cuts at: 229 5203 229
Size: 4974 2432
XbaI T'CTAG_A
Cuts at: 4437 4437
Size: 7406
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 270 574 2110 4901 270
Size: 304 1536 2791 2775
Fragments arranged by size:
2791 2775 1536 304
XmaI C'CCGG_G
Cuts at: 168 1322 4426 168
Size: 1154 3104 3148
Fragments arranged by size:
3148 3104 1154
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PasI PciI
PflMI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI AscI BaeI
BcgI BmgBI BmtI BsiWI BsmBI BsrGI BssHII BstZ17I
Bsu36I ClaI DraIII EcoNI FseI FspAI HpaI NdeI
NheI NotI NruI PacI PfoI PmlI PshAI PsiI
PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI
SwaI XhoI XmnI ZraI