Restriction map for
pF1KA1115
(Circular) (Six-base) MAPSORT of: pF1KA1115.seq Check: 3981 from: 1 to: 5756
pF1KA1115 5756 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 275 275
Size: 5756
AatII G_ACGT'C
Cuts at: 1444 1444
Size: 5756
AccI GT'mk_AC
Cuts at: 2794 2794
Size: 5756
Acc65I G'GTAC_C
Cuts at: 2772 2772
Size: 5756
AclI AA'CG_TT
Cuts at: 5596 5596
Size: 5756
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 462 966 1600 2503 3581 4013 4760 5028 462
Size: 504 634 903 1078 432 747 268 1190
Fragments arranged by size:
1190 1078 903 747 634 504 432 268
AflII C'TTAA_G
Cuts at: 861 861
Size: 5756
AflIII A'CryG_T
Cuts at: 869 3108 4212 5189 869
Size: 2239 1104 977 1436
Fragments arranged by size:
2239 1436 1104 977
AgeI A'CCGG_T
Cuts at: 4144 4144
Size: 5756
AleI CACnn'nnGTG
Cuts at: 622 622
Size: 5756
AlwNI CAG_nnn'CTG
Cuts at: 690 750 811 921 1098 1578 2376 3061 4628
Size: 60 61 110 177 480 798 685 1567
Cuts at: 4628 690
Size: 1818
Fragments arranged by size:
1818 1567 798 685 480 177 110 61
60
ApaI G_GGCC'C
Cuts at: 1026 1553 1634 2070 2342 2610 1026
Size: 527 81 436 272 268 4172
Fragments arranged by size:
4172 527 436 272 268 81
ApaLI G'TGCA_C
Cuts at: 4526 4526
Size: 5756
ApoI r'AATT_y
Cuts at: 2760 5109 5365 2760
Size: 2349 256 3151
Fragments arranged by size:
3151 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5756
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5756
AvaI C'yCGr_G
Cuts at: 181 2288 2776 181
Size: 2107 488 3161
Fragments arranged by size:
3161 2107 488
BamHI G'GATC_C
Cuts at: 911 1433 2537 2781 911
Size: 522 1104 244 3886
Fragments arranged by size:
3886 1104 522 244
BanI G'GyrC_C
Cuts at: 397 580 1051 1522 2035 2772 3186 3434 3469
Size: 183 471 471 513 737 414 248 35
Cuts at: 3469 397
Size: 2684
Fragments arranged by size:
2684 737 513 471 471 414 248 183
35
BanII G_rGCy'C
Cuts at: 941 1010 1026 1238 1553 1634 2011 2070 2342
Size: 69 16 212 315 81 377 59 272
Cuts at: 2342 2480 2610 2647 2654 2770 3800 941
Size: 138 130 37 7 116 1030 2897
Fragments arranged by size:
2897 1030 377 315 272 212 138 130
116 81 69 59 37 16 7
BbeI G_GCGC'C
Cuts at: 1055 2039 3190 3438 1055
Size: 984 1151 248 3373
Fragments arranged by size:
3373 1151 984 248
BbsI GAAGACnn'nnnn_
Cuts at: 206 1281 2159 206
Size: 1075 878 3803
Fragments arranged by size:
3803 1075 878
BbvCI CC'TCA_GC
Cuts at: 635 640 2222 2436 2481 635
Size: 5 1582 214 45 3910
Fragments arranged by size:
3910 1582 214 45 5
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 855 889 855
Size: 34 5722
BciVI GTATCCnnnnn_n'
Cuts at: 3654 4415 5756 3654
Size: 761 1341 3654
Fragments arranged by size:
3654 1341 761
BclI T'GATC_A
Cuts at: 3276 3276
Size: 5756
BglI GCCn_nnn'nGGC
Cuts at: 945 1021 2065 2605 2658 945
Size: 76 1044 540 53 4043
Fragments arranged by size:
4043 1044 540 76 53
BglII A'GATC_T
Cuts at: 3271 5308 3271
Size: 2037 3719
BlpI GC'TnA_GC
Cuts at: 1078 1339 2868 1078
Size: 261 1529 3966
Fragments arranged by size:
3966 1529 261
Bme1580I G_kGCm'C
Cuts at: 377 1026 1203 1256 1527 1553 1634 2070 2342
Size: 649 177 53 271 26 81 436 272
Cuts at: 2342 2354 2610 2682 3381 3474 4530 377
Size: 12 256 72 699 93 1056 1603
Fragments arranged by size:
1603 1056 699 649 436 272 271 256
177 93 81 72 53 26 12
BmgBI CAC'GTC
Cuts at: 204 1251 204
Size: 1047 4709
BmrI ACTGGGnnnn_n'
Cuts at: 335 738 2254 2327 2968 3049 3384 5528 335
Size: 403 1516 73 641 81 335 2144 563
Fragments arranged by size:
2144 1516 641 563 403 335 81 73
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4123 4123
Size: 5756
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 853 955 1024 1123 1138 1680 2213 2309
Size: 675 102 69 99 15 542 533 96
Cuts at: 2309 178
Size: 3625
Fragments arranged by size:
3625 675 542 533 102 99 96 69
15
Bpu10I CC'TnA_GC
Cuts at: 172 635 640 769 1204 2010 2222 2410 2436
Size: 463 5 129 435 806 212 188 26
Cuts at: 2436 2481 172
Size: 45 3447
Fragments arranged by size:
3447 806 463 435 212 188 129 45
26 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1705 1954 2932 3811 4303 4601 1705
Size: 249 978 879 492 298 2860
Fragments arranged by size:
2860 978 879 492 298 249
BsaI GGTCTCn'nnnn_
Cuts at: 1636 2420 2570 5460 1636
Size: 784 150 2890 1932
Fragments arranged by size:
2890 1932 784 150
BsaAI yAC'GTr
Cuts at: 1179 3109 1179
Size: 1930 3826
BsaBI GATnn'nnATC
Cuts at: 6 722 3270 5307 6
Size: 716 2548 2037 455
Fragments arranged by size:
2548 2037 716 455
BsaHI Gr'CG_yC
Cuts at: 1052 1375 1441 2036 2399 3187 3435 4137 5633
Size: 323 66 595 363 788 248 702 1496
Cuts at: 5633 1052
Size: 1175
Fragments arranged by size:
1496 1175 788 702 595 363 323 248
66
BsaWI w'CCGG_w
Cuts at: 2093 2945 3466 4144 4418 4565 4976 2093
Size: 852 521 678 274 147 411 2873
Fragments arranged by size:
2873 852 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1675 1705 2307 2337 2682 2712 1675
Size: 30 602 30 345 30 4719
Fragments arranged by size:
4719 602 345 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 331 685 1465 1683 1756 1843 1861 1999 2146
Size: 354 780 218 73 87 18 138 147
Cuts at: 2146 2155 2158 2161 2173 2547 2687 331
Size: 9 3 3 12 374 140 3400
Fragments arranged by size:
3400 780 374 354 218 147 140 138
87 73 18 12 9 3 3
BseYI C'CCAG_C
Cuts at: 1591 2209 2236 2551 2595 3181 3939 4516 5012
Size: 618 27 315 44 586 758 577 496
Cuts at: 5012 1591
Size: 2335
Fragments arranged by size:
2335 758 618 586 577 496 315 44
27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 112 1159 1552 2195 2296 112
Size: 1047 393 643 101 3572
Fragments arranged by size:
3572 1047 643 393 101
BsiEI CG_ry'CG
Cuts at: 104 1461 1716 3298 3344 4552 104
Size: 1357 255 1582 46 1208 1308
Fragments arranged by size:
1582 1357 1308 1208 255 46
BsiHKAI G_wGCw'C
Cuts at: 445 1010 1238 1333 2770 3548 3738 4530 445
Size: 565 228 95 1437 778 190 792 1671
Fragments arranged by size:
1671 1437 792 778 565 228 190 95
BsmBI CGTCTCn'nnnn_
Cuts at: 838 1366 838
Size: 528 5228
Bsp1286I G_dGCh'C
Cuts at: 377 445 941 1010 1026 1203 1238 1256 1333
Size: 68 496 69 16 177 35 18 77
Cuts at: 1333 1527 1553 1634 2011 2070 2342 2354 2480
Size: 194 26 81 377 59 272 12 126
Cuts at: 2480 2610 2647 2654 2682 2770 3381 3474 3548
Size: 130 37 7 28 88 611 93 74
Cuts at: 3548 3738 3800 4530 377
Size: 190 62 730 1603
Fragments arranged by size:
1603 730 611 496 377 272 194 190
177 130 126 93 88 81 77 74
69 68 62 59 37 35 28 26
18 16 12 7
BspEI T'CCGG_A
Cuts at: 2093 4976 2093
Size: 2883 2873
BspHI T'CATG_A
Cuts at: 4932 4932
Size: 5756
BspMI ACCTGCnnnn'nnnn_
Cuts at: 134 275 569 2806 3020 3322 3703 134
Size: 141 294 2237 214 302 381 2187
Fragments arranged by size:
2237 2187 381 302 294 214 141
BsrBI CCG'CTC
Cuts at: 50 162 321 630 1431 4048 4102 5586 5751
Size: 112 159 309 801 2617 54 1484 165
Cuts at: 5751 50
Size: 55
Fragments arranged by size:
2617 1484 801 309 165 159 112 55
54
BsrDI GCAATG_nn'
Cuts at: 281 3668 281
Size: 3387 2369
BsrFI r'CCGG_y
Cuts at: 419 445 1519 1715 3754 3935 4144 419
Size: 26 1074 196 2039 181 209 2031
Fragments arranged by size:
2039 2031 1074 209 196 181 26
BsrGI T'GTAC_A
Cuts at: 1464 1464
Size: 5756
BssSI C'ACGA_G
Cuts at: 523 1231 4027 4385 523
Size: 708 2796 358 1894
Fragments arranged by size:
2796 1894 708 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3133 3133
Size: 5756
BstBI TT'CG_AA
Cuts at: 4117 4117
Size: 5756
BstEII G'GTnAC_C
Cuts at: 288 1501 288
Size: 1213 4543
BstXI CCAn_nnnn'nTGG
Cuts at: 2086 2086
Size: 5756
BstYI r'GATC_y
Cuts at: 1 701 911 1433 2537 2781 3248 3271 3606
Size: 700 210 522 1104 244 467 23 335
Cuts at: 3606 3852 4864 4950 4962 5308 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1104 1012 700 522 467 449 346 335
246 244 210 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1739 2444 1739
Size: 705 5051
BtgI C'CryG_G
Cuts at: 1072 2181 2532 1072
Size: 1109 351 4296
Fragments arranged by size:
4296 1109 351
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3706 3887 4050 3706
Size: 181 163 5412
Fragments arranged by size:
5412 181 163
BtsI GCAGTG_nn'
Cuts at: 460 993 1420 1615 2999 460
Size: 533 427 195 1384 3217
Fragments arranged by size:
3217 1384 533 427 195
DraI TTT'AAA
Cuts at: 2755 2755
Size: 5756
DraIII CAC_nnn'GTG
Cuts at: 2089 2089
Size: 5756
DrdI GACnn_nn'nnGTC
Cuts at: 3462 4320 3462
Size: 858 4898
EaeI y'GGCC_r
Cuts at: 467 587 824 1193 1517 2057 2378 3341 3515
Size: 120 237 369 324 540 321 963 174
Cuts at: 3515 3906 3933 5278 467
Size: 391 27 1345 945
Fragments arranged by size:
1345 963 945 540 391 369 324 321
237 174 120 27
EagI C'GGCC_G
Cuts at: 3341 3341
Size: 5756
EarI CTCTTCn'nnn_
Cuts at: 1279 1976 1991 2123 2300 2635 3779 3989 1279
Size: 697 15 132 177 335 1144 210 3046
Fragments arranged by size:
3046 1144 697 335 210 177 132 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 754 1142 1654 3964 4274 4420 5566 754
Size: 388 512 2310 310 146 1146 944
Fragments arranged by size:
2310 1146 944 512 388 310 146
EcoICRI GAG'CTC
Cuts at: 1008 1236 2768 1008
Size: 228 1532 3996
Fragments arranged by size:
3996 1532 228
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 462 853 955 966 1024 1123 1138 1600
Size: 284 391 102 11 58 99 15 462
Cuts at: 1600 1680 2213 2309 2503 3581 4013 4760 5028
Size: 80 533 96 194 1078 432 747 268
Cuts at: 5028 178
Size: 906
Fragments arranged by size:
1078 906 747 533 462 432 391 284
268 194 102 99 96 80 58 15
11
EcoNI CCTnn'n_nnAGG
Cuts at: 1487 1487
Size: 5756
EcoO109I rG'GnC_Cy
Cuts at: 168 1022 1023 1480 1549 1630 1631 2066 2233
Size: 854 1 457 69 81 1 435 167
Cuts at: 2233 2338 2339 2358 2421 2514 2606 2685 2728
Size: 105 1 19 63 93 92 79 43
Cuts at: 2728 2895 168
Size: 167 3029
Fragments arranged by size:
3029 854 457 435 167 167 105 93
92 81 79 69 63 43 19 1
1 1
EcoRI G'AATT_C
Cuts at: 2760 2760
Size: 5756
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 567 3775 567
Size: 3208 2548
FspI TGC'GCA
Cuts at: 3537 3537
Size: 5756
HaeII r_GCGC'y
Cuts at: 1055 1211 2039 3190 3438 4460 5439 1055
Size: 156 828 1151 248 1022 979 1372
Fragments arranged by size:
1372 1151 1022 979 828 248 156
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 3598 3628 3630 3660 3598
Size: 30 2 30 5694
Fragments arranged by size:
5694 30 30 2
HincII GTy'rAC
Cuts at: 228 2795 228
Size: 2567 3189
KasI G'GCGC_C
Cuts at: 1051 2035 3186 3434 1051
Size: 984 1151 248 3373
Fragments arranged by size:
3373 1151 984 248
KpnI G_GTAC'C
Cuts at: 2776 2776
Size: 5756
MfeI C'AATT_G
Cuts at: 657 657
Size: 5756
MluI A'CGCG_T
Cuts at: 5189 5189
Size: 5756
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 758 4427 4611 758
Size: 3669 184 1903
Fragments arranged by size:
3669 1903 184
MscI TGG'CCA
Cuts at: 469 826 1195 2059 2380 3517 469
Size: 357 369 864 321 1137 2708
Fragments arranged by size:
2708 1137 864 369 357 321
MslI CAynn'nnrTG
Cuts at: 622 3745 3872 622
Size: 3123 127 2506
Fragments arranged by size:
3123 2506 127
MspA1I CmG'CkG
Cuts at: 639 687 1002 1074 1082 1566 1575 2055 2240
Size: 48 315 72 8 484 9 480 185
Cuts at: 2240 2867 3181 3541 4554 4799 639
Size: 627 314 360 1013 245 1596
Fragments arranged by size:
1596 1013 627 484 480 360 315 314
245 185 72 48 9 8
NaeI GCC'GGC
Cuts at: 3937 3937
Size: 5756
NarI GG'CG_CC
Cuts at: 1052 2036 3187 3435 1052
Size: 984 1151 248 3373
Fragments arranged by size:
3373 1151 984 248
NgoMIV G'CCGG_C
Cuts at: 3935 3935
Size: 5756
NspI r_CATG'y
Cuts at: 873 1146 1309 2809 3309 4216 873
Size: 273 163 1500 500 907 2413
Fragments arranged by size:
2413 1500 907 500 273 163
PasI CC'CwG_GG
Cuts at: 393 2004 2355 393
Size: 1611 351 3794
Fragments arranged by size:
3794 1611 351
PciI A'CATG_T
Cuts at: 869 4212 869
Size: 3343 2413
PflMI CCAn_nnn'nTGG
Cuts at: 113 822 2499 113
Size: 709 1677 3370
Fragments arranged by size:
3370 1677 709
PfoI T'CCnGG_A
Cuts at: 794 794
Size: 5756
PmeI GTTT'AAAC
Cuts at: 2755 2755
Size: 5756
PmlI CAC'GTG
Cuts at: 1179 1179
Size: 5756
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4895 4927 4895
Size: 32 5724
PpuMI rG'GwC_Cy
Cuts at: 168 2421 2514 168
Size: 2253 93 3410
Fragments arranged by size:
3410 2253 93
PspOMI G'GGCC_C
Cuts at: 1022 1549 1630 2066 2338 2606 1022
Size: 527 81 436 272 268 4172
Fragments arranged by size:
4172 527 436 272 268 81
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 409 441 409
Size: 32 5724
PstI C_TGCA'G
Cuts at: 212 257 440 572 1412 1757 2408 2803 3488
Size: 45 183 132 840 345 651 395 685
Cuts at: 3488 212
Size: 2480
Fragments arranged by size:
2480 840 685 651 395 345 183 132
45
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5756
PvuII CAG'CTG
Cuts at: 639 1002 1082 1566 1575 2055 2240 3181 3541
Size: 363 80 484 9 480 185 941 360
Cuts at: 3541 639
Size: 2854
Fragments arranged by size:
2854 941 484 480 363 360 185 80
9
RsrII CG'GwC_CG
Cuts at: 3951 3951
Size: 5756
SacI G_AGCT'C
Cuts at: 1010 1238 2770 1010
Size: 228 1532 3996
Fragments arranged by size:
3996 1532 228
SacII CC_GC'GG
Cuts at: 1075 1075
Size: 5756
SalI G'TCGA_C
Cuts at: 2793 2793
Size: 5756
SapI GCTCTTCn'nnn_
Cuts at: 2300 2635 3779 3989 2300
Size: 335 1144 210 4067
Fragments arranged by size:
4067 1144 335 210
SbfI CC_TGCA'GG
Cuts at: 2803 2803
Size: 5756
ScaI AGT'ACT
Cuts at: 5273 5273
Size: 5756
SexAI A'CCwGG_T
Cuts at: 1667 2085 1667
Size: 418 5338
SfcI C'TryA_G
Cuts at: 33 208 253 436 568 1126 1408 1753 2267
Size: 175 45 183 132 558 282 345 514
Cuts at: 2267 2404 2799 3484 4477 4668 33
Size: 137 395 685 993 191 1121
Fragments arranged by size:
1121 993 685 558 514 395 345 282
191 183 175 137 132 45
SfiI GGCCn_nnn'nGGCC
Cuts at: 1021 2065 1021
Size: 1044 4712
SfoI GGC'GCC
Cuts at: 1053 2037 3188 3436 1053
Size: 984 1151 248 3373
Fragments arranged by size:
3373 1151 984 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5756
SmaI CCC'GGG
Cuts at: 2778 2778
Size: 5756
SmlI C'TyrA_G
Cuts at: 861 1720 1933 2911 3826 4318 4580 861
Size: 859 213 978 915 492 262 2037
Fragments arranged by size:
2037 978 915 859 492 262 213
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5756
SphI G_CATG'C
Cuts at: 1146 1309 2809 3309 1146
Size: 163 1500 500 3593
Fragments arranged by size:
3593 1500 500 163
StuI AGG'CCT
Cuts at: 1762 2497 1762
Size: 735 5021
StyI C'CwwG_G
Cuts at: 1017 2498 2654 2890 3244 1017
Size: 1481 156 236 354 3529
Fragments arranged by size:
3529 1481 354 236 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 307 385 1693 3339 3472 3931 4140 307
Size: 78 1308 1646 133 459 209 1923
Fragments arranged by size:
1923 1646 1308 459 209 133 78
TatI w'GTAC_w
Cuts at: 1464 5271 1464
Size: 3807 1949
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1675 1707 2075 2107 2307 2339 2682 2714 5273
Size: 32 368 32 200 32 343 32 2559
Cuts at: 5273 5305 1675
Size: 32 2126
Fragments arranged by size:
2559 2126 368 343 200 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 3553 3553
Size: 5756
XbaI T'CTAG_A
Cuts at: 2787 2787
Size: 5756
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1263 1341 2237 2373 3251 1263
Size: 78 896 136 878 3768
Fragments arranged by size:
3768 896 878 136 78
XmaI C'CCGG_G
Cuts at: 2776 2776
Size: 5756
XmnI GAAnn'nnTTC
Cuts at: 976 976
Size: 5756
ZraI GAC'GTC
Cuts at: 1442 1442
Size: 5756
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AleI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AhdI AlfI AloI AscI AvrII BaeI BfrBI
BmtI BsiWI BsmI BssHII BstZ17I ClaI CspCI EcoRV
FseI FspAI HindIII HpaI NcoI NdeI NheI NotI
NruI NsiI PacI PshAI PsiI PspXI SanDI SgrAI
SnaBI SrfI SspI SwaI XhoI