Restriction map for
pF1KA1116
(Circular) (Six-base) MAPSORT of: pF1KA1116.seq Check: 7751 from: 1 to: 6926
pF1KA1116 6926 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AccI GT'mk_AC
Cuts at: 3080 3192 3964 3080
Size: 112 772 6042
Fragments arranged by size:
6042 772 112
Acc65I G'GTAC_C
Cuts at: 3942 3942
Size: 6926
AclI AA'CG_TT
Cuts at: 6766 6766
Size: 6926
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1202 1678 2426 4751 5183 5536 6198 1202
Size: 476 748 2325 432 353 662 1930
Fragments arranged by size:
2325 1930 748 662 476 432 353
AflIII A'CryG_T
Cuts at: 231 4278 6078 6359 231
Size: 4047 1800 281 798
Fragments arranged by size:
4047 1800 798 281
AgeI A'CCGG_T
Cuts at: 3155 3429 3155
Size: 274 6652
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3524 3556 3524
Size: 32 6894
AlwNI CAG_nnn'CTG
Cuts at: 988 1927 2404 3871 3910 4231 5669 988
Size: 939 477 1467 39 321 1438 2245
Fragments arranged by size:
2245 1467 1438 939 477 321 39
ApaLI G'TGCA_C
Cuts at: 5764 5764
Size: 6926
ApoI r'AATT_y
Cuts at: 399 953 2540 2582 2942 3653 3930 6279 6535
Size: 554 1587 42 360 711 277 2349 256
Cuts at: 6535 399
Size: 790
Fragments arranged by size:
2349 1587 790 711 554 360 277 256
42
AseI AT'TA_AT
Cuts at: 20 724 1625 20
Size: 704 901 5321
Fragments arranged by size:
5321 901 704
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6926
AvaI C'yCGr_G
Cuts at: 1640 3686 3946 1640
Size: 2046 260 4620
Fragments arranged by size:
4620 2046 260
BamHI G'GATC_C
Cuts at: 3951 3951
Size: 6926
BanI G'GyrC_C
Cuts at: 2043 2907 3942 4356 4604 4639 2043
Size: 864 1035 414 248 35 4330
Fragments arranged by size:
4330 1035 864 414 248 35
BanII G_rGCy'C
Cuts at: 1692 1960 3940 4970 1692
Size: 268 1980 1030 3648
Fragments arranged by size:
3648 1980 1030 268
BbeI G_GCGC'C
Cuts at: 2047 4360 4608 2047
Size: 2313 248 4365
Fragments arranged by size:
4365 2313 248
BbsI GAAGACnn'nnnn_
Cuts at: 127 926 3075 3297 3573 127
Size: 799 2149 222 276 3480
Fragments arranged by size:
3480 2149 799 276 222
BciVI GTATCCnnnnn_n'
Cuts at: 2854 3101 4824 5880 6926 2854
Size: 247 1723 1056 1046 2854
Fragments arranged by size:
2854 1723 1056 1046 247
BclI T'GATC_A
Cuts at: 1878 4446 1878
Size: 2568 4358
BfrBI ATG'CAT
Cuts at: 1681 1681
Size: 6926
BglI GCCn_nnn'nGGC
Cuts at: 112 3387 112
Size: 3275 3651
BglII A'GATC_T
Cuts at: 686 1510 4441 6478 686
Size: 824 2931 2037 1134
Fragments arranged by size:
2931 2037 1134 824
BlpI GC'TnA_GC
Cuts at: 3776 4038 3776
Size: 262 6664
Bme1580I G_kGCm'C
Cuts at: 4551 4644 5768 4551
Size: 93 1124 5709
Fragments arranged by size:
5709 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 2681 4138 4219 4554 6698 2681
Size: 1457 81 335 2144 2909
Fragments arranged by size:
2909 2144 1457 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 593 1979 3791 5293 593
Size: 1386 1812 1502 2226
Fragments arranged by size:
2226 1812 1502 1386
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 512 563 1949 2177 3005 512
Size: 51 1386 228 828 4433
Fragments arranged by size:
4433 1386 828 228 51
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 686 1284 1395 4102 4981 5695 5993 686
Size: 598 111 2707 879 714 298 1619
Fragments arranged by size:
2707 1619 879 714 598 298 111
BsaI GGTCTCn'nnnn_
Cuts at: 3347 6630 3347
Size: 3283 3643
BsaAI yAC'GTr
Cuts at: 4279 4279
Size: 6926
BsaBI GATnn'nnATC
Cuts at: 6 1799 4440 6477 6
Size: 1793 2641 2037 455
Fragments arranged by size:
2641 2037 1793 455
BsaHI Gr'CG_yC
Cuts at: 2044 4357 4605 5307 6803 2044
Size: 2313 248 702 1496 2167
Fragments arranged by size:
2313 2167 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2277 3155 3429 4115 4636 5314 5725 5872 6146
Size: 878 274 686 521 678 411 147 274
Cuts at: 6146 2277
Size: 3057
Fragments arranged by size:
3057 878 686 678 521 411 274 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 843 873 843
Size: 30 6896
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1085 1273 2003 2096 3410 3668 1085
Size: 188 730 93 1314 258 4343
Fragments arranged by size:
4343 1314 730 258 188 93
BseYI C'CCAG_C
Cuts at: 758 4351 5109 5774 6182 758
Size: 3593 758 665 408 1502
Fragments arranged by size:
3593 1502 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2275 2275
Size: 6926
BsiEI CG_ry'CG
Cuts at: 104 1441 4468 4514 5744 104
Size: 1337 3027 46 1230 1286
Fragments arranged by size:
3027 1337 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1692 3940 4718 4908 5768 1692
Size: 2248 778 190 860 2850
Fragments arranged by size:
2850 2248 860 778 190
BsmI GAATG_Cn'
Cuts at: 1131 1131
Size: 6926
BsmBI CGTCTCn'nnnn_
Cuts at: 1930 1930
Size: 6926
Bsp1286I G_dGCh'C
Cuts at: 1692 1960 3940 4551 4644 4718 4908 4970 5768
Size: 268 1980 611 93 74 190 62 798
Cuts at: 5768 1692
Size: 2850
Fragments arranged by size:
2850 1980 798 611 268 190 93 74
62
BspHI T'CATG_A
Cuts at: 5358 5358
Size: 6926
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3976 4190 4492 4873 3976
Size: 214 302 381 6029
Fragments arranged by size:
6029 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1409 2356 5218 5272 6756 6921 50
Size: 1359 947 2862 54 1484 165 55
Fragments arranged by size:
2862 1484 1359 947 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4838 4838
Size: 6926
BsrFI r'CCGG_y
Cuts at: 2097 3155 3429 4924 5105 2097
Size: 1058 274 1495 181 3918
Fragments arranged by size:
3918 1495 1058 274 181
BssSI C'ACGA_G
Cuts at: 5197 5905 5197
Size: 708 6218
BstAPI GCAn_nnn'nTGC
Cuts at: 4303 4303
Size: 6926
BstBI TT'CG_AA
Cuts at: 172 5287 172
Size: 5115 1811
BstYI r'GATC_y
Cuts at: 1 686 1510 1800 3237 3951 4418 4441 4776
Size: 685 824 290 1437 714 467 23 335
Cuts at: 4776 5022 5328 5340 5426 6478 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1437 1052 824 714 685 467 449 335
306 290 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1097 3743 1097
Size: 2646 4280
BtgI C'CryG_G
Cuts at: 107 637 1822 3006 107
Size: 530 1185 1184 4027
Fragments arranged by size:
4027 1185 1184 530
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4876 5057 5220 4876
Size: 181 163 6582
Fragments arranged by size:
6582 181 163
BtsI GCAGTG_nn'
Cuts at: 560 4169 560
Size: 3609 3317
ClaI AT'CG_AT
Cuts at: 3492 3492
Size: 6926
DraI TTT'AAA
Cuts at: 1757 3925 1757
Size: 2168 4758
DraIII CAC_nnn'GTG
Cuts at: 1525 2689 3007 1525
Size: 1164 318 5444
Fragments arranged by size:
5444 1164 318
DrdI GACnn_nn'nnGTC
Cuts at: 4632 5976 4632
Size: 1344 5582
EaeI y'GGCC_r
Cuts at: 548 608 2596 4511 4685 5076 5103 6448 548
Size: 60 1988 1915 174 391 27 1345 1026
Fragments arranged by size:
1988 1915 1345 1026 391 174 60 27
EagI C'GGCC_G
Cuts at: 4511 4511
Size: 6926
EarI CTCTTCn'nnn_
Cuts at: 1250 1595 1697 2996 3345 3850 4949 5159 1250
Size: 345 102 1299 349 505 1099 210 3017
Fragments arranged by size:
3017 1299 1099 505 349 345 210 102
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 512 5134 5876 6022 6736 512
Size: 4622 742 146 714 702
Fragments arranged by size:
4622 742 714 702 146
EcoICRI GAG'CTC
Cuts at: 1690 3938 1690
Size: 2248 4678
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 512 563 1202 1678 1949 2177 2426 3005 4751
Size: 51 639 476 271 228 249 579 1746
Cuts at: 4751 5183 5536 6198 512
Size: 432 353 662 1240
Fragments arranged by size:
1746 1240 662 639 579 476 432 353
271 249 228 51
EcoNI CCTnn'n_nnAGG
Cuts at: 3576 3576
Size: 6926
EcoO109I rG'GnC_Cy
Cuts at: 777 3250 4065 777
Size: 2473 815 3638
Fragments arranged by size:
3638 2473 815
EcoRI G'AATT_C
Cuts at: 2540 2942 3653 3930 2540
Size: 402 711 277 5536
Fragments arranged by size:
5536 711 402 277
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2942 3530 4945 2942
Size: 588 1415 4923
Fragments arranged by size:
4923 1415 588
FspI TGC'GCA
Cuts at: 4707 4707
Size: 6926
HaeII r_GCGC'y
Cuts at: 2047 4360 4608 5838 6609 2047
Size: 2313 248 1230 771 2364
Fragments arranged by size:
2364 2313 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 510 542 1046 1078 3402 3434 4768 4798 4800
Size: 32 504 32 2324 32 1334 30 2
Cuts at: 4800 4830 510
Size: 30 2606
Fragments arranged by size:
2606 2324 1334 504 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 3040 3965 3040
Size: 925 6001
KasI G'GCGC_C
Cuts at: 2043 4356 4604 2043
Size: 2313 248 4365
Fragments arranged by size:
4365 2313 248
KpnI G_GTAC'C
Cuts at: 3946 3946
Size: 6926
MluI A'CGCG_T
Cuts at: 6359 6359
Size: 6926
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2277 2410 5685 5869 2277
Size: 133 3275 184 3334
Fragments arranged by size:
3334 3275 184 133
MscI TGG'CCA
Cuts at: 550 4687 550
Size: 4137 2789
MslI CAynn'nnrTG
Cuts at: 565 1627 4915 5042 565
Size: 1062 3288 127 2449
Fragments arranged by size:
3288 2449 1062 127
MspA1I CmG'CkG
Cuts at: 832 838 844 2365 2815 4037 4351 4711 5495
Size: 6 6 1521 450 1222 314 360 784
Cuts at: 5495 5740 832
Size: 245 2018
Fragments arranged by size:
2018 1521 1222 784 450 360 314 245
6 6
NaeI GCC'GGC
Cuts at: 5107 5107
Size: 6926
NarI GG'CG_CC
Cuts at: 2044 4357 4605 2044
Size: 2313 248 4365
Fragments arranged by size:
4365 2313 248
NcoI C'CATG_G
Cuts at: 107 1822 107
Size: 1715 5211
NgoMIV G'CCGG_C
Cuts at: 5105 5105
Size: 6926
NsiI A_TGCA'T
Cuts at: 1683 1683
Size: 6926
NspI r_CATG'y
Cuts at: 235 3775 3979 4479 6082 235
Size: 3540 204 500 1603 1079
Fragments arranged by size:
3540 1603 1079 500 204
PasI CC'CwG_GG
Cuts at: 415 3383 3572 415
Size: 2968 189 3769
Fragments arranged by size:
3769 2968 189
PciI A'CATG_T
Cuts at: 231 6078 231
Size: 5847 1079
PflMI CCAn_nnn'nTGG
Cuts at: 2176 2815 3007 3271 2176
Size: 639 192 264 5831
Fragments arranged by size:
5831 639 264 192
PfoI T'CCnGG_A
Cuts at: 579 2109 2787 2889 3021 3063 3252 579
Size: 1530 678 102 132 42 189 4253
Fragments arranged by size:
4253 1530 678 189 132 102 42
PmeI GTTT'AAAC
Cuts at: 3925 3925
Size: 6926
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5372 5404 5372
Size: 32 6894
PpuMI rG'GwC_Cy
Cuts at: 3250 3250
Size: 6926
PshAI GACnn'nnGTC
Cuts at: 1984 2885 1984
Size: 901 6025
PstI C_TGCA'G
Cuts at: 776 837 843 3619 3973 4658 776
Size: 61 6 2776 354 685 3044
Fragments arranged by size:
3044 2776 685 354 61 6
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6926
PvuII CAG'CTG
Cuts at: 832 838 844 2365 2815 4351 4711 832
Size: 6 6 1521 450 1536 360 3047
Fragments arranged by size:
3047 1536 1521 450 360 6 6
RsrII CG'GwC_CG
Cuts at: 5121 5121
Size: 6926
SacI G_AGCT'C
Cuts at: 1692 3940 1692
Size: 2248 4678
SalI G'TCGA_C
Cuts at: 3963 3963
Size: 6926
SapI GCTCTTCn'nnn_
Cuts at: 1697 2996 4949 5159 1697
Size: 1299 1953 210 3464
Fragments arranged by size:
3464 1953 1299 210
SbfI CC_TGCA'GG
Cuts at: 3973 3973
Size: 6926
ScaI AGT'ACT
Cuts at: 446 1536 6443 446
Size: 1090 4907 929
Fragments arranged by size:
4907 1090 929
SexAI A'CCwGG_T
Cuts at: 2175 2175
Size: 6926
SfcI C'TryA_G
Cuts at: 33 677 772 833 839 2057 3167 3233 3299
Size: 644 95 61 6 1218 1110 66 66
Cuts at: 3299 3615 3969 4654 5622 5813 33
Size: 316 354 685 968 191 1146
Fragments arranged by size:
1218 1146 1110 968 685 644 354 316
191 95 66 66 61 6
SfoI GGC'GCC
Cuts at: 2045 4358 4606 2045
Size: 2313 248 4365
Fragments arranged by size:
4365 2313 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6926
SmaI CCC'GGG
Cuts at: 1642 3948 1642
Size: 2306 4620
SmlI C'TyrA_G
Cuts at: 701 1299 1410 4081 4996 5710 5972 701
Size: 598 111 2671 915 714 262 1655
Fragments arranged by size:
2671 1655 915 714 598 262 111
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6926
SphI G_CATG'C
Cuts at: 3979 4479 3979
Size: 500 6426
SspI AAT'ATT
Cuts at: 2649 2669 2649
Size: 20 6906
StyI C'CwwG_G
Cuts at: 107 621 1730 1822 3514 4060 4414 107
Size: 514 1109 92 1692 546 354 2619
Fragments arranged by size:
2619 1692 1109 546 514 354 92
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 180 378 2700 3467 4509 4642 5101 5310 180
Size: 198 2322 767 1042 133 459 209 1796
Fragments arranged by size:
2322 1796 1042 767 459 209 198 133
TatI w'GTAC_w
Cuts at: 444 1534 2760 3795 6441 444
Size: 1090 1226 1035 2646 929
Fragments arranged by size:
2646 1226 1090 1035 929
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 575 607 843 875 3654 3686 6443 6475 575
Size: 32 236 32 2779 32 2757 32 1026
Fragments arranged by size:
2779 2757 1026 236 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4723 4723
Size: 6926
XbaI T'CTAG_A
Cuts at: 1372 3957 1372
Size: 2585 4341
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2443 3380 4421 2443
Size: 937 1041 4948
Fragments arranged by size:
4948 1041 937
XmaI C'CCGG_G
Cuts at: 1640 3946 1640
Size: 2306 4620
XmnI GAAnn'nnTTC
Cuts at: 124 1314 124
Size: 1190 5736
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI
ClaI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbvCI BcgI BmgBI BmtI Bpu10I
BsiWI BspEI BsrGI BssHII BstEII BstXI BstZ17I CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI PacI PmlI PsiI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI ZraI