Restriction map for
pF1KA1121
(Circular) (Six-base) MAPSORT of: pF1KA1121.seq Check: 5928 from: 1 to: 7172
pF1KA1121 7172 bp
With 182 enzymes: SgfI *
March 2, 2010 14:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3032 3032
Size: 7172
AatII G_ACGT'C
Cuts at: 1050 1050
Size: 7172
AccI GT'mk_AC
Cuts at: 1050 2520 2900 4210 1050
Size: 1470 380 1310 4012
Fragments arranged by size:
4012 1470 1310 380
Acc65I G'GTAC_C
Cuts at: 4188 4188
Size: 7172
AclI AA'CG_TT
Cuts at: 2439 7012 2439
Size: 4573 2599
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 691 1156 2546 2770 3313 3380 3415 4997 5429
Size: 465 1390 224 543 67 35 1582 432
Cuts at: 5429 5782 6444 691
Size: 353 662 1419
Fragments arranged by size:
1582 1419 1390 662 543 465 432 353
224 67 35
AflIII A'CryG_T
Cuts at: 1118 1763 2817 4524 6324 6605 1118
Size: 645 1054 1707 1800 281 1685
Fragments arranged by size:
1800 1707 1685 1054 645 281
AgeI A'CCGG_T
Cuts at: 581 581
Size: 7172
AhdI GACnn_n'nnGTC
Cuts at: 120 120
Size: 7172
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 190 190
Size: 7172
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 959 991 1258 1290 959
Size: 32 267 32 6841
Fragments arranged by size:
6841 267 32 32
AlwNI CAG_nnn'CTG
Cuts at: 564 2967 4477 5915 564
Size: 2403 1510 1438 1821
Fragments arranged by size:
2403 1821 1510 1438
ApaLI G'TGCA_C
Cuts at: 6010 6010
Size: 7172
ApoI r'AATT_y
Cuts at: 826 1084 2107 3010 4176 6525 6781 826
Size: 258 1023 903 1166 2349 256 1217
Fragments arranged by size:
2349 1217 1166 1023 903 258 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7172
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7172
AvaI C'yCGr_G
Cuts at: 728 3781 4192 728
Size: 3053 411 3708
Fragments arranged by size:
3708 3053 411
BamHI G'GATC_C
Cuts at: 4197 4197
Size: 7172
BanI G'GyrC_C
Cuts at: 262 268 1438 2329 3246 3292 4188 4602 4850
Size: 6 1170 891 917 46 896 414 248
Cuts at: 4850 4885 262
Size: 35 2549
Fragments arranged by size:
2549 1170 917 896 891 414 248 46
35 6
BanII G_rGCy'C
Cuts at: 232 814 2088 4186 5216 232
Size: 582 1274 2098 1030 2188
Fragments arranged by size:
2188 2098 1274 1030 582
BbeI G_GCGC'C
Cuts at: 266 272 2333 4606 4854 266
Size: 6 2061 2273 248 2584
Fragments arranged by size:
2584 2273 2061 248 6
BbsI GAAGACnn'nnnn_
Cuts at: 729 4157 4166 729
Size: 3428 9 3735
Fragments arranged by size:
3735 3428 9
BbvCI CC'TCA_GC
Cuts at: 790 2045 790
Size: 1255 5917
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 2273 2307 90
Size: 34 2149 34 4955
Fragments arranged by size:
4955 2149 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1110 1194 5070 6126 7172 1110
Size: 84 3876 1056 1046 1110
Fragments arranged by size:
3876 1110 1056 1046 84
BclI T'GATC_A
Cuts at: 1407 3882 4692 1407
Size: 2475 810 3887
Fragments arranged by size:
3887 2475 810
BglI GCCn_nnn'nGGC
Cuts at: 359 359
Size: 7172
BglII A'GATC_T
Cuts at: 1626 3303 4687 6724 1626
Size: 1677 1384 2037 2074
Fragments arranged by size:
2074 2037 1677 1384
BlpI GC'TnA_GC
Cuts at: 808 4284 808
Size: 3476 3696
Bme1580I G_kGCm'C
Cuts at: 1441 2000 4797 4890 6014 1441
Size: 559 2797 93 1124 2599
Fragments arranged by size:
2797 2599 1124 559 93
BmgBI CAC'GTC
Cuts at: 3792 3792
Size: 7172
BmrI ACTGGGnnnn_n'
Cuts at: 2584 4384 4465 4800 6944 2584
Size: 1800 81 335 2144 2812
Fragments arranged by size:
2812 2144 1800 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5539 5539
Size: 7172
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 360 1192 2915 360
Size: 832 1723 4617
Fragments arranged by size:
4617 1723 832
Bpu10I CC'TnA_GC
Cuts at: 790 2045 790
Size: 1255 5917
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4348 5227 5941 6239 4348
Size: 879 714 298 5281
Fragments arranged by size:
5281 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 6876 6876
Size: 7172
BsaAI yAC'GTr
Cuts at: 3807 4525 3807
Size: 718 6454
BsaBI GATnn'nnATC
Cuts at: 6 4686 6723 6
Size: 4680 2037 455
Fragments arranged by size:
4680 2037 455
BsaHI Gr'CG_yC
Cuts at: 263 269 1047 1511 2330 3323 3800 4603 4851
Size: 6 778 464 819 993 477 803 248
Cuts at: 4851 5553 7049 263
Size: 702 1496 386
Fragments arranged by size:
1496 993 819 803 778 702 477 464
386 248 6
BsaWI w'CCGG_w
Cuts at: 581 3932 4061 4361 4882 5560 5971 6118 6392
Size: 3351 129 300 521 678 411 147 274
Cuts at: 6392 581
Size: 1361
Fragments arranged by size:
3351 1361 678 521 411 300 274 147
129
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 266 296 959 989 1123 1153 1258 1288 1895
Size: 30 663 30 134 30 105 30 607
Cuts at: 1895 1925 2024 2054 3883 3913 266
Size: 30 99 30 1829 30 3525
Fragments arranged by size:
3525 1829 663 607 134 105 99 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 169 172 454 457 460 1144 1270 1403 2617
Size: 3 282 3 3 684 126 133 1214
Cuts at: 2617 2812 3871 169
Size: 195 1059 3470
Fragments arranged by size:
3470 1214 1059 684 282 195 133 126
3 3 3
BseYI C'CCAG_C
Cuts at: 211 249 346 382 1853 4597 5355 6020 6428
Size: 38 97 36 1471 2744 758 665 408
Cuts at: 6428 211
Size: 955
Fragments arranged by size:
2744 1471 955 758 665 408 97 38
36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 308 667 1768 1797 3334 3900 308
Size: 359 1101 29 1537 566 3580
Fragments arranged by size:
3580 1537 1101 566 359 29
BsiEI CG_ry'CG
Cuts at: 104 236 369 681 4714 4760 5990 104
Size: 132 133 312 4033 46 1230 1286
Fragments arranged by size:
4033 1286 1230 312 133 132 46
BsiHKAI G_wGCw'C
Cuts at: 179 814 1507 2088 2804 4186 4964 5154 6014
Size: 635 693 581 716 1382 778 190 860
Cuts at: 6014 179
Size: 1337
Fragments arranged by size:
1382 1337 860 778 716 693 635 581
190
BsmI GAATG_Cn'
Cuts at: 1677 1677
Size: 7172
BsmBI CGTCTCn'nnnn_
Cuts at: 2869 4075 2869
Size: 1206 5966
Bsp1286I G_dGCh'C
Cuts at: 179 232 814 1441 1507 2000 2088 2804 4186
Size: 53 582 627 66 493 88 716 1382
Cuts at: 4186 4797 4890 4964 5154 5216 6014 179
Size: 611 93 74 190 62 798 1337
Fragments arranged by size:
1382 1337 798 716 627 611 582 493
190 93 88 74 66 62 53
BspEI T'CCGG_A
Cuts at: 4061 4061
Size: 7172
BspHI T'CATG_A
Cuts at: 635 5604 635
Size: 4969 2203
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1160 1740 3032 3356 4222 4436 4738 5119 1160
Size: 580 1292 324 866 214 302 381 3213
Fragments arranged by size:
3213 1292 866 580 381 324 302 214
BsrBI CCG'CTC
Cuts at: 50 164 426 3111 5464 5518 7002 7167 50
Size: 114 262 2685 2353 54 1484 165 55
Fragments arranged by size:
2685 2353 1484 262 165 114 55 54
BsrDI GCAATG_nn'
Cuts at: 3009 5084 3009
Size: 2075 5097
BsrFI r'CCGG_y
Cuts at: 235 265 368 536 581 1179 2717 5170 5351
Size: 30 103 168 45 598 1538 2453 181
Cuts at: 5351 235
Size: 2056
Fragments arranged by size:
2453 2056 1538 598 181 168 103 45
30
BsrGI T'GTAC_A
Cuts at: 1121 3863 1121
Size: 2742 4430
BssHII G'CGCG_C
Cuts at: 197 199 197
Size: 2 7170
BssSI C'ACGA_G
Cuts at: 5443 6151 5443
Size: 708 6464
BstAPI GCAn_nnn'nTGC
Cuts at: 4549 4549
Size: 7172
BstBI TT'CG_AA
Cuts at: 965 5533 965
Size: 4568 2604
BstXI CCAn_nnnn'nTGG
Cuts at: 528 1110 2389 2939 3765 528
Size: 582 1279 550 826 3935
Fragments arranged by size:
3935 1279 826 582 550
BstYI r'GATC_y
Cuts at: 1 1626 3063 3303 4197 4664 4687 5022 5268
Size: 1625 1437 240 894 467 23 335 246
Cuts at: 5268 5574 5586 5672 6724 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1625 1437 1052 894 467 449 335 306
246 240 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1805 1805
Size: 7172
BtgI C'CryG_G
Cuts at: 843 859 1973 2388 843
Size: 16 1114 415 5627
Fragments arranged by size:
5627 1114 415 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 477 4157 5122 5303 5466 477
Size: 3680 965 181 163 2183
Fragments arranged by size:
3680 2183 965 181 163
BtsI GCAGTG_nn'
Cuts at: 1468 4415 1468
Size: 2947 4225
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1361 1396 1361
Size: 35 7137
DraI TTT'AAA
Cuts at: 4171 4171
Size: 7172
DrdI GACnn_nn'nnGTC
Cuts at: 2883 4878 6222 2883
Size: 1995 1344 3833
Fragments arranged by size:
3833 1995 1344
EaeI y'GGCC_r
Cuts at: 233 366 821 1160 1872 2226 3794 4757 4931
Size: 133 455 339 712 354 1568 963 174
Cuts at: 4931 5322 5349 6694 233
Size: 391 27 1345 711
Fragments arranged by size:
1568 1345 963 712 711 455 391 354
339 174 133 27
EagI C'GGCC_G
Cuts at: 233 366 4757 233
Size: 133 4391 2648
Fragments arranged by size:
4391 2648 133
EarI CTCTTCn'nnn_
Cuts at: 410 451 667 751 845 1711 2121 2681 3359
Size: 41 216 84 94 866 410 560 678
Cuts at: 3359 5195 5405 410
Size: 1836 210 2177
Fragments arranged by size:
2177 1836 866 678 560 410 216 210
94 84 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 705 3808 5380 6122 6268 6982 705
Size: 3103 1572 742 146 714 895
Fragments arranged by size:
3103 1572 895 742 714 146
EcoICRI GAG'CTC
Cuts at: 812 2086 4184 812
Size: 1274 2098 3800
Fragments arranged by size:
3800 2098 1274
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 360 691 1156 1192 2546 2770 2915 3313 3380
Size: 331 465 36 1354 224 145 398 67
Cuts at: 3380 3415 4997 5429 5782 6444 360
Size: 35 1582 432 353 662 1088
Fragments arranged by size:
1582 1354 1088 662 465 432 398 353
331 224 145 67 36 35
EcoNI CCTnn'n_nnAGG
Cuts at: 793 793
Size: 7172
EcoO109I rG'GnC_Cy
Cuts at: 855 3345 4020 4311 855
Size: 2490 675 291 3716
Fragments arranged by size:
3716 2490 675 291
EcoRI G'AATT_C
Cuts at: 4176 4176
Size: 7172
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5191 5191
Size: 7172
FseI GG_CCGG'CC
Cuts at: 372 372
Size: 7172
FspI TGC'GCA
Cuts at: 766 4953 766
Size: 4187 2985
HaeII r_GCGC'y
Cuts at: 245 266 272 2333 4606 4854 6084 6855 245
Size: 21 6 2061 2273 248 1230 771 562
Fragments arranged by size:
2273 2061 1230 771 562 248 21 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 106 138 1123 1155 3100 3132 3271 3303 5014
Size: 32 985 32 1945 32 139 32 1711
Cuts at: 5014 5044 5046 5076 106
Size: 30 2 30 2202
Fragments arranged by size:
2202 1945 1711 985 139 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1149 2521 2652 3207 3926 4211 1149
Size: 1372 131 555 719 285 4110
Fragments arranged by size:
4110 1372 719 555 285 131
HindIII A'AGCT_T
Cuts at: 2740 2740
Size: 7172
HpaI GTT'AAC
Cuts at: 3207 3207
Size: 7172
KasI G'GCGC_C
Cuts at: 262 268 2329 4602 4850 262
Size: 6 2061 2273 248 2584
Fragments arranged by size:
2584 2273 2061 248 6
KpnI G_GTAC'C
Cuts at: 4192 4192
Size: 7172
MfeI C'AATT_G
Cuts at: 1819 3080 1819
Size: 1261 5911
MluI A'CGCG_T
Cuts at: 6605 6605
Size: 7172
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 401 1502 2144 5931 6115 401
Size: 1101 642 3787 184 1458
Fragments arranged by size:
3787 1458 1101 642 184
MscI TGG'CCA
Cuts at: 823 1162 2228 4933 823
Size: 339 1066 2705 3062
Fragments arranged by size:
3062 2705 1066 339
MslI CAynn'nnrTG
Cuts at: 526 1246 2387 3252 3571 3907 4129 5161 5288
Size: 720 1141 865 319 336 222 1032 127
Cuts at: 5288 526
Size: 2410
Fragments arranged by size:
2410 1141 1032 865 720 336 319 222
127
MspA1I CmG'CkG
Cuts at: 308 320 468 564 861 996 1477 2049 2964
Size: 12 148 96 297 135 481 572 915
Cuts at: 2964 4283 4597 4957 5741 5986 308
Size: 1319 314 360 784 245 1494
Fragments arranged by size:
1494 1319 915 784 572 481 360 314
297 245 148 135 96 12
NaeI GCC'GGC
Cuts at: 237 267 370 538 5353 237
Size: 30 103 168 4815 2056
Fragments arranged by size:
4815 2056 168 103 30
NarI GG'CG_CC
Cuts at: 263 269 2330 4603 4851 263
Size: 6 2061 2273 248 2584
Fragments arranged by size:
2584 2273 2061 248 6
NcoI C'CATG_G
Cuts at: 2388 2388
Size: 7172
NgoMIV G'CCGG_C
Cuts at: 235 265 368 536 5351 235
Size: 30 103 168 4815 2056
Fragments arranged by size:
4815 2056 168 103 30
NspI r_CATG'y
Cuts at: 1122 1179 2821 2872 4225 4725 6328 1122
Size: 57 1642 51 1353 500 1603 1966
Fragments arranged by size:
1966 1642 1603 1353 500 57 51
PasI CC'CwG_GG
Cuts at: 1443 1443
Size: 7172
PciI A'CATG_T
Cuts at: 1118 2817 6324 1118
Size: 1699 3507 1966
Fragments arranged by size:
3507 1966 1699
PflMI CCAn_nnn'nTGG
Cuts at: 618 618
Size: 7172
PfoI T'CCnGG_A
Cuts at: 814 4022 814
Size: 3208 3964
PmeI GTTT'AAAC
Cuts at: 4171 4171
Size: 7172
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 959 991 1258 1290 5618 5650 959
Size: 32 267 32 4328 32 2481
Fragments arranged by size:
4328 2481 267 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 855 3345 4020 855
Size: 2490 675 4007
Fragments arranged by size:
4007 2490 675
PshAI GACnn'nnGTC
Cuts at: 2008 3814 2008
Size: 1806 5366
PstI C_TGCA'G
Cuts at: 434 467 569 4121 4219 4904 434
Size: 33 102 3552 98 685 2702
Fragments arranged by size:
3552 2702 685 102 98 33
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7172
PvuII CAG'CTG
Cuts at: 468 564 996 1477 2049 2964 4597 4957 468
Size: 96 432 481 572 915 1633 360 2683
Fragments arranged by size:
2683 1633 915 572 481 432 360 96
RsrII CG'GwC_CG
Cuts at: 3930 5367 3930
Size: 1437 5735
SacI G_AGCT'C
Cuts at: 814 2088 4186 814
Size: 1274 2098 3800
Fragments arranged by size:
3800 2098 1274
SacII CC_GC'GG
Cuts at: 862 862
Size: 7172
SalI G'TCGA_C
Cuts at: 2519 4209 2519
Size: 1690 5482
SapI GCTCTTCn'nnn_
Cuts at: 667 5195 5405 667
Size: 4528 210 2434
Fragments arranged by size:
4528 2434 210
SbfI CC_TGCA'GG
Cuts at: 4219 4219
Size: 7172
ScaI AGT'ACT
Cuts at: 6689 6689
Size: 7172
SexAI A'CCwGG_T
Cuts at: 3919 3919
Size: 7172
SfcI C'TryA_G
Cuts at: 33 430 463 565 1399 2358 3996 4117 4215
Size: 397 33 102 834 959 1638 121 98
Cuts at: 4215 4900 5868 6059 33
Size: 685 968 191 1146
Fragments arranged by size:
1638 1146 968 959 834 685 397 191
121 102 98 33
SfoI GGC'GCC
Cuts at: 264 270 2331 4604 4852 264
Size: 6 2061 2273 248 2584
Fragments arranged by size:
2584 2273 2061 248 6
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7172
SgrAI Cr'CCGG_yG
Cuts at: 265 265
Size: 7172
SmaI CCC'GGG
Cuts at: 730 3783 4194 730
Size: 3053 411 3708
Fragments arranged by size:
3708 3053 411
SmlI C'TyrA_G
Cuts at: 4327 5242 5956 6218 4327
Size: 915 714 262 5281
Fragments arranged by size:
5281 915 714 262
SpeI A'CTAG_T
Cuts at: 67 2769 67
Size: 2702 4470
SphI G_CATG'C
Cuts at: 1179 2872 4225 4725 1179
Size: 1693 1353 500 3626
Fragments arranged by size:
3626 1693 1353 500
StuI AGG'CCT
Cuts at: 1334 2400 1334
Size: 1066 6106
StyI C'CwwG_G
Cuts at: 1329 2027 2388 4306 4660 1329
Size: 698 361 1918 354 3841
Fragments arranged by size:
3841 1918 698 361 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 274 1456 4755 4888 5347 5556 274
Size: 1182 3299 133 459 209 1890
Fragments arranged by size:
3299 1890 1182 459 209 133
TatI w'GTAC_w
Cuts at: 1121 1760 2238 3863 6687 1121
Size: 639 478 1625 2824 1606
Fragments arranged by size:
2824 1625 1606 639 478
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 266 298 1219 1251 1895 1927 6689 6721 266
Size: 32 921 32 644 32 4762 32 717
Fragments arranged by size:
4762 921 717 644 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4969 4969
Size: 7172
XbaI T'CTAG_A
Cuts at: 4203 4203
Size: 7172
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 390 946 1245 4667 390
Size: 556 299 3422 2895
Fragments arranged by size:
3422 2895 556 299
XmaI C'CCGG_G
Cuts at: 728 3781 4192 728
Size: 3053 411 3708
Fragments arranged by size:
3708 3053 411
XmnI GAAnn'nnTTC
Cuts at: 1492 2783 1492
Size: 1291 5881
ZraI GAC'GTC
Cuts at: 1048 1048
Size: 7172
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AflII AleI ApaI AscI AvrII BaeI BfrBI
BmtI BsiWI BstEII BstZ17I ClaI DraIII EcoRV FspAI
NdeI NheI NotI NruI NsiI PacI PmlI PsiI
PspOMI PspXI PsrI SanDI SfiI SnaBI SrfI SspI
SwaI XhoI