Restriction map for
pF1KA1124
(Circular) (Six-base) MAPSORT of: pF1KA1124.seq Check: 1873 from: 1 to: 8246
pF1KA1124 8246 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4141 4902 4141
Size: 761 7485
AatII G_ACGT'C
Cuts at: 4565 4565
Size: 8246
AccI GT'mk_AC
Cuts at: 5284 5284
Size: 8246
Acc65I G'GTAC_C
Cuts at: 5262 5262
Size: 8246
AclI AA'CG_TT
Cuts at: 8086 8086
Size: 8246
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1058 1372 3524 4413 4652 6071 6503 7250 7518
Size: 314 2152 889 239 1419 432 747 268
Cuts at: 7518 1058
Size: 1786
Fragments arranged by size:
2152 1786 1419 889 747 432 314 268
239
AflIII A'CryG_T
Cuts at: 2723 3299 5598 6702 7679 2723
Size: 576 2299 1104 977 3290
Fragments arranged by size:
3290 2299 1104 977 576
AgeI A'CCGG_T
Cuts at: 6634 6634
Size: 8246
AhdI GACnn_n'nnGTC
Cuts at: 900 900
Size: 8246
AleI CACnn'nnGTG
Cuts at: 3433 3433
Size: 8246
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1504 4930 1504
Size: 3426 4820
AlwNI CAG_nnn'CTG
Cuts at: 1065 1689 2517 2718 2926 3153 3230 4242 4265
Size: 624 828 201 208 227 77 1012 23
Cuts at: 4265 4749 5551 7118 1065
Size: 484 802 1567 2193
Fragments arranged by size:
2193 1567 1012 828 802 624 484 227
208 201 77 23
ApaI G_GGCC'C
Cuts at: 161 1539 4897 4972 161
Size: 1378 3358 75 3435
Fragments arranged by size:
3435 3358 1378 75
ApaLI G'TGCA_C
Cuts at: 697 3659 4015 7016 697
Size: 2962 356 3001 1927
Fragments arranged by size:
3001 2962 1927 356
ApoI r'AATT_y
Cuts at: 407 607 2885 2911 5250 7599 7855 407
Size: 200 2278 26 2339 2349 256 798
Fragments arranged by size:
2349 2339 2278 798 256 200 26
AseI AT'TA_AT
Cuts at: 20 20
Size: 8246
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8246
AvaI C'yCGr_G
Cuts at: 274 1532 1627 2600 2644 3343 3911 4695 5266
Size: 1258 95 973 44 699 568 784 571
Cuts at: 5266 274
Size: 3254
Fragments arranged by size:
3254 1258 973 784 699 571 568 95
44
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1016 1049 1016
Size: 33 8213
BamHI G'GATC_C
Cuts at: 5271 5271
Size: 8246
BanI G'GyrC_C
Cuts at: 2128 3316 4129 4636 5262 5676 5924 5959 2128
Size: 1188 813 507 626 414 248 35 4415
Fragments arranged by size:
4415 1188 813 626 507 414 248 35
BanII G_rGCy'C
Cuts at: 161 1539 1868 2645 2798 2933 3580 3893 4403
Size: 1378 329 777 153 135 647 313 510
Cuts at: 4403 4487 4897 4972 5179 5260 6290 161
Size: 84 410 75 207 81 1030 2117
Fragments arranged by size:
2117 1378 1030 777 647 510 410 329
313 207 153 135 84 81 75
BbeI G_GCGC'C
Cuts at: 5680 5928 5680
Size: 248 7998
BbsI GAAGACnn'nnnn_
Cuts at: 335 620 4770 335
Size: 285 4150 3811
Fragments arranged by size:
4150 3811 285
BbvCI CC'TCA_GC
Cuts at: 1540 2021 3769 1540
Size: 481 1748 6017
Fragments arranged by size:
6017 1748 481
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 304 338 2578 2612 304
Size: 34 2240 34 5938
Fragments arranged by size:
5938 2240 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6144 6905 8246 6144
Size: 761 1341 6144
Fragments arranged by size:
6144 1341 761
BclI T'GATC_A
Cuts at: 5766 5766
Size: 8246
BglI GCCn_nnn'nGGC
Cuts at: 112 2758 112
Size: 2646 5600
BglII A'GATC_T
Cuts at: 5761 7798 5761
Size: 2037 6209
BlpI GC'TnA_GC
Cuts at: 601 1477 2000 3015 4297 5358 601
Size: 876 523 1015 1282 1061 3489
Fragments arranged by size:
3489 1282 1061 1015 876 523
Bme1580I G_kGCm'C
Cuts at: 161 701 823 1539 1875 3321 3334 3663 4019
Size: 540 122 716 336 1446 13 329 356
Cuts at: 4019 4473 4897 4946 4972 5871 5964 7020 161
Size: 454 424 49 26 899 93 1056 1387
Fragments arranged by size:
1446 1387 1056 899 716 540 454 424
356 336 329 122 93 49 26 13
BmgBI CAC'GTC
Cuts at: 204 735 1221 1233 2218 3375 4533 204
Size: 531 486 12 985 1157 1158 3917
Fragments arranged by size:
3917 1158 1157 985 531 486 12
BmrI ACTGGGnnnn_n'
Cuts at: 2556 2936 5458 5539 5874 8018 2556
Size: 380 2522 81 335 2144 2784
Fragments arranged by size:
2784 2522 2144 380 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4723 6613 4723
Size: 1890 6356
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 1429 1492 1720 2110 2197 2518 2758 2776
Size: 1272 63 228 390 87 321 240 18
Cuts at: 2776 3192 3727 5239 157
Size: 416 535 1512 3164
Fragments arranged by size:
3164 1512 1272 535 416 390 321 240
228 87 63 18
Bpu10I CC'TnA_GC
Cuts at: 181 1540 1981 2021 2797 3769 4252 181
Size: 1359 441 40 776 972 483 4175
Fragments arranged by size:
4175 1359 972 776 483 441 40
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 112 1660 2083 4099 5422 6301 6793 7091 112
Size: 1548 423 2016 1323 879 492 298 1267
Fragments arranged by size:
2016 1548 1323 1267 879 492 423 298
BsaI GGTCTCn'nnnn_
Cuts at: 451 908 964 4823 7950 451
Size: 457 56 3859 3127 747
Fragments arranged by size:
3859 3127 747 457 56
BsaAI yAC'GTr
Cuts at: 261 696 3300 4449 4881 5599 261
Size: 435 2604 1149 432 718 2908
Fragments arranged by size:
2908 2604 1149 718 435 432
BsaBI GATnn'nnATC
Cuts at: 6 1805 3985 5760 7797 6
Size: 1799 2180 1775 2037 455
Fragments arranged by size:
2180 2037 1799 1775 455
BsaHI Gr'CG_yC
Cuts at: 1946 2813 4562 5677 5925 6627 8123 1946
Size: 867 1749 1115 248 702 1496 2069
Fragments arranged by size:
2069 1749 1496 1115 867 702 248
BsaWI w'CCGG_w
Cuts at: 624 4074 5435 5956 6634 6908 7055 7466 624
Size: 3450 1361 521 678 274 147 411 1404
Fragments arranged by size:
3450 1404 1361 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2649 2679 2731 2761 3024 3054 3220 3250 4474
Size: 30 52 30 263 30 166 30 1224
Cuts at: 4474 4504 2649
Size: 30 6391
Fragments arranged by size:
6391 1224 263 166 52 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 490 1455 1750 1927 1930 2377 2670 2806 2972
Size: 965 295 177 3 447 293 136 166
Cuts at: 2972 3982 4426 4639 490
Size: 1010 444 213 4097
Fragments arranged by size:
4097 1010 965 447 444 295 293 213
177 166 136 3
BseYI C'CCAG_C
Cuts at: 1089 3518 5161 5671 6429 7006 7502 1089
Size: 2429 1643 510 758 577 496 1833
Fragments arranged by size:
2429 1833 1643 758 577 510 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 249 508 820 1417 1531 1726 3216 3243 3397
Size: 259 312 597 114 195 1490 27 154
Cuts at: 3397 4255 4612 249
Size: 858 357 3883
Fragments arranged by size:
3883 1490 858 597 357 312 259 195
154 114 27
BsiEI CG_ry'CG
Cuts at: 104 5788 5834 7042 104
Size: 5684 46 1208 1308
Fragments arranged by size:
5684 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 209 701 1415 1868 2054 2144 2645 2798 3580
Size: 492 714 453 186 90 501 153 782
Cuts at: 3580 3663 4019 4253 4403 4487 4919 5260 6038
Size: 83 356 234 150 84 432 341 778
Cuts at: 6038 6228 7020 209
Size: 190 792 1435
Fragments arranged by size:
1435 792 782 778 714 501 492 453
432 356 341 234 190 186 153 150
90 84 83
Bsp1286I G_dGCh'C
Cuts at: 161 209 701 823 1415 1539 1868 1875 2054
Size: 48 492 122 592 124 329 7 179
Cuts at: 2054 2144 2645 2798 2933 3321 3334 3580 3663
Size: 90 501 153 135 388 13 246 83
Cuts at: 3663 3893 4019 4253 4403 4473 4487 4897 4919
Size: 230 126 234 150 70 14 410 22
Cuts at: 4919 4946 4972 5179 5260 5871 5964 6038 6228
Size: 27 26 207 81 611 93 74 190
Cuts at: 6228 6290 7020 161
Size: 62 730 1387
Fragments arranged by size:
1387 730 611 592 501 492 410 388
329 246 234 230 207 190 179 153
150 135 126 124 122 93 90 83
81 74 70 62 48 27 26 22
14 13 7
BspEI T'CCGG_A
Cuts at: 624 4074 7466 624
Size: 3450 3392 1404
Fragments arranged by size:
3450 3392 1404
BspHI T'CATG_A
Cuts at: 986 7422 986
Size: 6436 1810
BspMI ACCTGCnnnn'nnnn_
Cuts at: 4141 4427 4902 4934 5296 5510 5812 6193 4141
Size: 286 475 32 362 214 302 381 6194
Fragments arranged by size:
6194 475 381 362 302 286 214 32
BsrBI CCG'CTC
Cuts at: 50 1770 2125 4048 4702 4975 6538 6592 8076
Size: 1720 355 1923 654 273 1563 54 1484
Cuts at: 8076 8241 50
Size: 165 55
Fragments arranged by size:
1923 1720 1563 1484 654 355 273 165
55 54
BsrDI GCAATG_nn'
Cuts at: 422 3530 6158 422
Size: 3108 2628 2510
Fragments arranged by size:
3108 2628 2510
BsrFI r'CCGG_y
Cuts at: 1085 3764 5226 6244 6425 6634 1085
Size: 2679 1462 1018 181 209 2697
Fragments arranged by size:
2697 2679 1462 1018 209 181
BsrGI T'GTAC_A
Cuts at: 218 4541 218
Size: 4323 3923
BssHII G'CGCG_C
Cuts at: 3453 4475 3453
Size: 1022 7224
BssSI C'ACGA_G
Cuts at: 964 5025 6517 6875 964
Size: 4061 1492 358 2335
Fragments arranged by size:
4061 2335 1492 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1419 1689 1962 3230 4749 5623 1419
Size: 270 273 1268 1519 874 4042
Fragments arranged by size:
4042 1519 1268 874 273 270
BstBI TT'CG_AA
Cuts at: 6607 6607
Size: 8246
BstEII G'GTnAC_C
Cuts at: 2605 3906 2605
Size: 1301 6945
BstXI CCAn_nnnn'nTGG
Cuts at: 4986 4986
Size: 8246
BstYI r'GATC_y
Cuts at: 1 845 2514 2765 3549 4175 4843 5271 5738
Size: 844 1669 251 784 626 668 428 467
Cuts at: 5738 5761 6096 6342 7354 7440 7452 7798 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1669 1012 844 784 668 626 467 449
428 346 335 251 246 86 23 12
BtgI C'CryG_G
Cuts at: 809 815 1521 3428 4027 4103 4580 809
Size: 6 706 1907 599 76 477 4475
Fragments arranged by size:
4475 1907 706 599 477 76 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 497 871 2422 6196 6377 6540 497
Size: 374 1551 3774 181 163 2203
Fragments arranged by size:
3774 2203 1551 374 181 163
BtsI GCAGTG_nn'
Cuts at: 2975 3228 4243 5489 2975
Size: 253 1015 1246 5732
Fragments arranged by size:
5732 1246 1015 253
DraI TTT'AAA
Cuts at: 5245 5245
Size: 8246
DraIII CAC_nnn'GTG
Cuts at: 227 1716 4014 227
Size: 1489 2298 4459
Fragments arranged by size:
4459 2298 1489
DrdI GACnn_nn'nnGTC
Cuts at: 208 1512 5952 6810 208
Size: 1304 4440 858 1644
Fragments arranged by size:
4440 1644 1304 858
EaeI y'GGCC_r
Cuts at: 113 263 665 812 1928 3999 4100 4106 4145
Size: 150 402 147 1116 2071 101 6 39
Cuts at: 4145 5831 6005 6396 6423 7768 113
Size: 1686 174 391 27 1345 591
Fragments arranged by size:
2071 1686 1345 1116 591 402 391 174
150 147 101 39 27 6
EagI C'GGCC_G
Cuts at: 5831 5831
Size: 8246
EarI CTCTTCn'nnn_
Cuts at: 351 992 1348 2195 2633 3490 4790 6269 6479
Size: 641 356 847 438 857 1300 1479 210
Cuts at: 6479 351
Size: 2118
Fragments arranged by size:
2118 1479 1300 857 847 641 438 356
210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 739 2776 6454 6764 6910 8056 739
Size: 2037 3678 310 146 1146 929
Fragments arranged by size:
3678 2037 1146 929 310 146
EcoICRI GAG'CTC
Cuts at: 1866 2643 2796 3578 4401 4485 5258 1866
Size: 777 153 782 823 84 773 4854
Fragments arranged by size:
4854 823 782 777 773 153 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 1058 1372 1429 1492 1720 2110 2197 2518
Size: 901 314 57 63 228 390 87 321
Cuts at: 2518 2758 2776 3192 3524 3727 4413 4652 5239
Size: 240 18 416 332 203 686 239 587
Cuts at: 5239 6071 6503 7250 7518 157
Size: 832 432 747 268 885
Fragments arranged by size:
901 885 832 747 686 587 432 416
390 332 321 314 268 240 239 228
203 87 63 57 18
EcoO109I rG'GnC_Cy
Cuts at: 158 1050 1404 1536 2094 3150 3417 4619 4785
Size: 892 354 132 558 1056 267 1202 166
Cuts at: 4785 4969 5385 158
Size: 184 416 3019
Fragments arranged by size:
3019 1202 1056 892 558 416 354 267
184 166 132
EcoRI G'AATT_C
Cuts at: 5250 5250
Size: 8246
EcoRV GAT'ATC
Cuts at: 4856 4856
Size: 8246
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 380 637 2020 2205 6265 380
Size: 257 1383 185 4060 2361
Fragments arranged by size:
4060 2361 1383 257 185
FspI TGC'GCA
Cuts at: 4576 4753 6027 4576
Size: 177 1274 6795
Fragments arranged by size:
6795 1274 177
FspAI rTGC'GCAy
Cuts at: 4576 4576
Size: 8246
HaeII r_GCGC'y
Cuts at: 179 1657 5680 5928 6950 7929 179
Size: 1478 4023 248 1022 979 496
Fragments arranged by size:
4023 1478 1022 979 496 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 751 783 820 852 886 918 1330 1362 2649
Size: 32 37 32 34 32 412 32 1287
Cuts at: 2649 2681 2731 2763 3633 3665 3905 3937 4206
Size: 32 50 32 870 32 240 32 269
Cuts at: 4206 4238 5052 5084 5107 5139 6088 6118 6120
Size: 32 814 32 23 32 949 30 2
Cuts at: 6120 6150 751
Size: 30 2847
Fragments arranged by size:
2847 1287 949 870 814 412 269 240
50 37 34 32 32 32 32 32
32 32 32 32 32 32 30 30
23 2
HincII GTy'rAC
Cuts at: 5285 5285
Size: 8246
HindIII A'AGCT_T
Cuts at: 619 1430 2041 4069 619
Size: 811 611 2028 4796
Fragments arranged by size:
4796 2028 811 611
KasI G'GCGC_C
Cuts at: 5676 5924 5676
Size: 248 7998
KpnI G_GTAC'C
Cuts at: 5266 5266
Size: 8246
MluI A'CGCG_T
Cuts at: 7679 7679
Size: 8246
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1235 1394 4751 4883 5180 6917 7101 1235
Size: 159 3357 132 297 1737 184 2380
Fragments arranged by size:
3357 2380 1737 297 184 159 132
MscI TGG'CCA
Cuts at: 667 1930 4102 6007 667
Size: 1263 2172 1905 2906
Fragments arranged by size:
2906 2172 1905 1263
MslI CAynn'nnrTG
Cuts at: 3433 4912 6235 6362 3433
Size: 1479 1323 127 5317
Fragments arranged by size:
5317 1479 1323 127
MspA1I CmG'CkG
Cuts at: 144 300 1401 1686 1899 2286 2496 2691 3127
Size: 156 1101 285 213 387 210 195 436
Cuts at: 3127 3844 3934 5165 5357 5671 6031 7044 7289
Size: 717 90 1231 192 314 360 1013 245
Cuts at: 7289 144
Size: 1101
Fragments arranged by size:
1231 1101 1101 1013 717 436 387 360
314 285 245 213 210 195 192 156
90
NaeI GCC'GGC
Cuts at: 1087 5228 6427 1087
Size: 4141 1199 2906
Fragments arranged by size:
4141 2906 1199
NarI GG'CG_CC
Cuts at: 5677 5925 5677
Size: 248 7998
NcoI C'CATG_G
Cuts at: 4580 4580
Size: 8246
NdeI CA'TA_TG
Cuts at: 3459 3459
Size: 8246
NgoMIV G'CCGG_C
Cuts at: 1085 5226 6425 1085
Size: 4141 1199 2906
Fragments arranged by size:
4141 2906 1199
NspI r_CATG'y
Cuts at: 921 1126 2727 3790 4911 5299 5799 6706 921
Size: 205 1601 1063 1121 388 500 907 2461
Fragments arranged by size:
2461 1601 1121 1063 907 500 388 205
PasI CC'CwG_GG
Cuts at: 3971 3971
Size: 8246
PciI A'CATG_T
Cuts at: 2723 6702 2723
Size: 3979 4267
PflMI CCAn_nnn'nTGG
Cuts at: 1303 2286 2718 3546 4098 4586 1303
Size: 983 432 828 552 488 4963
Fragments arranged by size:
4963 983 828 552 488 432
PfoI T'CCnGG_A
Cuts at: 149 2786 5005 149
Size: 2637 2219 3390
Fragments arranged by size:
3390 2637 2219
PmeI GTTT'AAAC
Cuts at: 5245 5245
Size: 8246
PmlI CAC'GTG
Cuts at: 3300 4881 3300
Size: 1581 6665
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7385 7417 7385
Size: 32 8214
PpuMI rG'GwC_Cy
Cuts at: 1050 1404 3417 4785 1050
Size: 354 2013 1368 4511
Fragments arranged by size:
4511 2013 1368 354
PshAI GACnn'nnGTC
Cuts at: 3744 3744
Size: 8246
PspOMI G'GGCC_C
Cuts at: 157 1535 4893 4968 157
Size: 1378 3358 75 3435
Fragments arranged by size:
3435 3358 1378 75
PspXI vC'TCGA_Gb
Cuts at: 274 1627 2644 274
Size: 1353 1017 5876
Fragments arranged by size:
5876 1353 1017
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5002 5034 5002
Size: 32 8214
PstI C_TGCA'G
Cuts at: 854 1075 1424 1832 2297 2513 2747 4808 5293
Size: 221 349 408 465 216 234 2061 485
Cuts at: 5293 5978 854
Size: 685 3122
Fragments arranged by size:
3122 2061 685 485 465 408 349 234
221 216
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8246
PvuII CAG'CTG
Cuts at: 144 300 1899 2286 2496 3844 5671 6031 144
Size: 156 1599 387 210 1348 1827 360 2359
Fragments arranged by size:
2359 1827 1599 1348 387 360 210 156
RsrII CG'GwC_CG
Cuts at: 6441 6441
Size: 8246
SacI G_AGCT'C
Cuts at: 1868 2645 2798 3580 4403 4487 5260 1868
Size: 777 153 782 823 84 773 4854
Fragments arranged by size:
4854 823 782 777 773 153 84
SalI G'TCGA_C
Cuts at: 5283 5283
Size: 8246
SapI GCTCTTCn'nnn_
Cuts at: 992 1348 2633 3490 6269 6479 992
Size: 356 1285 857 2779 210 2759
Fragments arranged by size:
2779 2759 1285 857 356 210
SbfI CC_TGCA'GG
Cuts at: 854 1832 5293 854
Size: 978 3461 3807
Fragments arranged by size:
3807 3461 978
ScaI AGT'ACT
Cuts at: 7763 7763
Size: 8246
SexAI A'CCwGG_T
Cuts at: 4328 4328
Size: 8246
SfcI C'TryA_G
Cuts at: 33 850 1071 1420 1828 2293 2509 2743 4512
Size: 817 221 349 408 465 216 234 1769
Cuts at: 4512 4804 5289 5974 6967 7158 33
Size: 292 485 685 993 191 1121
Fragments arranged by size:
1769 1121 993 817 685 485 465 408
349 292 234 221 216 191
SfoI GGC'GCC
Cuts at: 5678 5926 5678
Size: 248 7998
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8246
SmaI CCC'GGG
Cuts at: 2602 5268 2602
Size: 2666 5580
SmlI C'TyrA_G
Cuts at: 127 274 1627 1639 2098 2644 4114 5401 6316
Size: 147 1353 12 459 546 1470 1287 915
Cuts at: 6316 6808 7070 127
Size: 492 262 1303
Fragments arranged by size:
1470 1353 1303 1287 915 546 492 459
262 147 12
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8246
SphI G_CATG'C
Cuts at: 1126 4911 5299 5799 1126
Size: 3785 388 500 3573
Fragments arranged by size:
3785 3573 500 388
SspI AAT'ATT
Cuts at: 1253 1253
Size: 8246
StuI AGG'CCT
Cuts at: 2026 2224 3360 5216 2026
Size: 198 1136 1856 5056
Fragments arranged by size:
5056 1856 1136 198
StyI C'CwwG_G
Cuts at: 116 1787 2030 2825 3794 4580 5380 5734 116
Size: 1671 243 795 969 786 800 354 2628
Fragments arranged by size:
2628 1671 969 800 795 786 354 243
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 568 898 1516 2650 3183 3532 4543 4578 5829
Size: 330 618 1134 533 349 1011 35 1251
Cuts at: 5829 5962 6421 6630 568
Size: 133 459 209 2184
Fragments arranged by size:
2184 1251 1134 1011 618 533 459 349
330 209 133 35
TatI w'GTAC_w
Cuts at: 218 4541 7761 218
Size: 4323 3220 703
Fragments arranged by size:
4323 3220 703
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2677 2709 3220 3252 4433 4465 4474 4506 7763
Size: 32 511 32 1181 32 9 32 3257
Cuts at: 7763 7795 2677
Size: 32 3128
Fragments arranged by size:
3257 3128 1181 511 32 32 32 32
32 9
Tth111I GACn'n_nGTC
Cuts at: 721 6043 721
Size: 5322 2924
XbaI T'CTAG_A
Cuts at: 3732 5277 3732
Size: 1545 6701
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1218 1938 2823 3150 3360 5741 1218
Size: 720 885 327 210 2381 3723
Fragments arranged by size:
3723 2381 885 720 327 210
XhoI C'TCGA_G
Cuts at: 274 1627 2644 274
Size: 1353 1017 5876
Fragments arranged by size:
5876 1353 1017
XmaI C'CCGG_G
Cuts at: 2600 5266 2600
Size: 2666 5580
XmnI GAAnn'nnTTC
Cuts at: 2570 4192 2570
Size: 1622 6624
ZraI GAC'GTC
Cuts at: 4563 4563
Size: 8246
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI FspAI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AloI AscI AvrII BfrBI BmtI BsiWI
BsmI BsmBI BstZ17I Bsu36I ClaI CspCI EcoNI FseI
HpaI MfeI NheI NotI NruI NsiI PacI PsiI
SacII SanDI SfiI SgrAI SnaBI SrfI SwaI