Restriction map for
pF1KA1128
(Circular) (Six-base) MAPSORT of: pF1KA1128.seq Check: 7551 from: 1 to: 6254
pF1KA1128 6254 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1143 1143
Size: 6254
AccI GT'mk_AC
Cuts at: 2798 3292 2798
Size: 494 5760
Acc65I G'GTAC_C
Cuts at: 2901 3270 2901
Size: 369 5885
AclI AA'CG_TT
Cuts at: 6094 6094
Size: 6254
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1470 2320 2699 2755 2974 4079 4511 4864 5526
Size: 850 379 56 219 1105 432 353 662
Cuts at: 5526 1470
Size: 2198
Fragments arranged by size:
2198 1105 850 662 432 379 353 219
56
AflIII A'CryG_T
Cuts at: 399 1144 1640 2583 2971 3246 3606 5406 5687
Size: 745 496 943 388 275 360 1800 281
Cuts at: 5687 399
Size: 966
Fragments arranged by size:
1800 966 943 745 496 388 360 281
275
AlwNI CAG_nnn'CTG
Cuts at: 2917 3033 3559 4997 2917
Size: 116 526 1438 4174
Fragments arranged by size:
4174 1438 526 116
ApaLI G'TGCA_C
Cuts at: 332 2558 5092 332
Size: 2226 2534 1494
Fragments arranged by size:
2534 2226 1494
ApoI r'AATT_y
Cuts at: 366 559 795 924 1128 2426 3258 5607 5863
Size: 193 236 129 204 1298 832 2349 256
Cuts at: 5863 366
Size: 757
Fragments arranged by size:
2349 1298 832 757 256 236 204 193
129
AseI AT'TA_AT
Cuts at: 20 746 20
Size: 726 5528
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6254
AvaI C'yCGr_G
Cuts at: 2782 3274 2782
Size: 492 5762
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1263 1296 1263
Size: 33 6221
BamHI G'GATC_C
Cuts at: 3279 3279
Size: 6254
BanI G'GyrC_C
Cuts at: 2901 3270 3684 3932 3967 2901
Size: 369 414 248 35 5188
Fragments arranged by size:
5188 414 369 248 35
BanII G_rGCy'C
Cuts at: 3268 4298 3268
Size: 1030 5224
BbeI G_GCGC'C
Cuts at: 3688 3936 3688
Size: 248 6006
BbsI GAAGACnn'nnnn_
Cuts at: 1349 1379 3220 1349
Size: 30 1841 4383
Fragments arranged by size:
4383 1841 30
BbvCI CC'TCA_GC
Cuts at: 1927 1927
Size: 6254
BciVI GTATCCnnnnn_n'
Cuts at: 4152 5208 6254 4152
Size: 1056 1046 4152
Fragments arranged by size:
4152 1056 1046
BclI T'GATC_A
Cuts at: 3774 3774
Size: 6254
BfrBI ATG'CAT
Cuts at: 2547 2547
Size: 6254
BglII A'GATC_T
Cuts at: 3769 5806 3769
Size: 2037 4217
BlpI GC'TnA_GC
Cuts at: 714 3366 714
Size: 2652 3602
Bme1580I G_kGCm'C
Cuts at: 336 2562 3879 3972 5096 336
Size: 2226 1317 93 1124 1494
Fragments arranged by size:
2226 1494 1317 1124 93
BmgBI CAC'GTC
Cuts at: 2586 2586
Size: 6254
BmrI ACTGGGnnnn_n'
Cuts at: 3466 3547 3882 6026 3466
Size: 81 335 2144 3694
Fragments arranged by size:
3694 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4621 4621
Size: 6254
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 725 1382 2439 2894 725
Size: 657 1057 455 4085
Fragments arranged by size:
4085 1057 657 455
Bpu10I CC'TnA_GC
Cuts at: 1175 1927 1175
Size: 752 5502
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 395 1774 1989 2420 2693 3430 4309 5023 5321
Size: 1379 215 431 273 737 879 714 298
Cuts at: 5321 395
Size: 1328
Fragments arranged by size:
1379 1328 879 737 714 431 298 273
215
BsaI GGTCTCn'nnnn_
Cuts at: 5958 5958
Size: 6254
BsaAI yAC'GTr
Cuts at: 3607 3607
Size: 6254
BsaBI GATnn'nnATC
Cuts at: 6 1763 1781 3768 5805 6
Size: 1757 18 1987 2037 455
Fragments arranged by size:
2037 1987 1757 455 18
BsaHI Gr'CG_yC
Cuts at: 3685 3933 4635 6131 3685
Size: 248 702 1496 3808
Fragments arranged by size:
3808 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2565 3443 3964 4642 5053 5200 5474 2565
Size: 878 521 678 411 147 274 3345
Fragments arranged by size:
3345 878 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1521 1551 1521
Size: 30 6224
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 529 2423 529
Size: 1894 4360
BseYI C'CCAG_C
Cuts at: 3679 4437 5102 5510 3679
Size: 758 665 408 4423
Fragments arranged by size:
4423 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 3796 3842 5072 104
Size: 3692 46 1230 1286
Fragments arranged by size:
3692 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 336 2562 3268 4046 4236 5096 336
Size: 2226 706 778 190 860 1494
Fragments arranged by size:
2226 1494 860 778 706 190
BsmI GAATG_Cn'
Cuts at: 2533 3189 2533
Size: 656 5598
BsmBI CGTCTCn'nnnn_
Cuts at: 2164 2415 2164
Size: 251 6003
Bsp1286I G_dGCh'C
Cuts at: 336 2562 3268 3879 3972 4046 4236 4298 5096
Size: 2226 706 611 93 74 190 62 798
Cuts at: 5096 336
Size: 1494
Fragments arranged by size:
2226 1494 798 706 611 190 93 74
62
BspEI T'CCGG_A
Cuts at: 2565 2565
Size: 6254
BspHI T'CATG_A
Cuts at: 4686 4686
Size: 6254
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1143 3304 3518 3820 4201 1143
Size: 2161 214 302 381 3196
Fragments arranged by size:
3196 2161 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4546 4600 6084 6249 50
Size: 4496 54 1484 165 55
Fragments arranged by size:
4496 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 184 2188 2209 4166 184
Size: 2004 21 1957 2272
Fragments arranged by size:
2272 2004 1957 21
BsrFI r'CCGG_y
Cuts at: 4252 4433 4252
Size: 181 6073
BsrGI T'GTAC_A
Cuts at: 1537 1537
Size: 6254
BssSI C'ACGA_G
Cuts at: 4525 5233 4525
Size: 708 5546
BstAPI GCAn_nnn'nTGC
Cuts at: 195 2528 3631 195
Size: 2333 1103 2818
Fragments arranged by size:
2818 2333 1103
BstBI TT'CG_AA
Cuts at: 4615 4615
Size: 6254
BstXI CCAn_nnnn'nTGG
Cuts at: 1687 1687
Size: 6254
BstYI r'GATC_y
Cuts at: 1 852 1782 2318 3279 3746 3769 4104 4350
Size: 851 930 536 961 467 23 335 246
Cuts at: 4350 4656 4668 4754 5806 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 961 930 851 536 467 449 335
306 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1790 1868 3060 1790
Size: 78 1192 4984
Fragments arranged by size:
4984 1192 78
BtgI C'CryG_G
Cuts at: 107 1953 107
Size: 1846 4408
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4204 4385 4548 4204
Size: 181 163 5910
Fragments arranged by size:
5910 181 163
BtsI GCAGTG_nn'
Cuts at: 2123 2529 3497 2123
Size: 406 968 4880
Fragments arranged by size:
4880 968 406
ClaI AT'CG_AT
Cuts at: 3216 3216
Size: 6254
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 480 515 2639 2674 480
Size: 35 2124 35 4060
Fragments arranged by size:
4060 2124 35 35
DraI TTT'AAA
Cuts at: 2876 3253 2876
Size: 377 5877
DraIII CAC_nnn'GTG
Cuts at: 340 340
Size: 6254
DrdI GACnn_nn'nnGTC
Cuts at: 3960 5304 3960
Size: 1344 4910
EaeI y'GGCC_r
Cuts at: 3839 4013 4404 4431 5776 3839
Size: 174 391 27 1345 4317
Fragments arranged by size:
4317 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3839 3839
Size: 6254
EarI CTCTTCn'nnn_
Cuts at: 341 1415 2612 2774 4277 4487 341
Size: 1074 1197 162 1503 210 2108
Fragments arranged by size:
2108 1503 1197 1074 210 162
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2822 3040 4462 5204 5350 6064 2822
Size: 218 1422 742 146 714 3012
Fragments arranged by size:
3012 1422 742 714 218 146
EcoICRI GAG'CTC
Cuts at: 3266 3266
Size: 6254
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 725 1382 1470 2320 2439 2699 2755 2894 2974
Size: 657 88 850 119 260 56 139 80
Cuts at: 2974 4079 4511 4864 5526 725
Size: 1105 432 353 662 1453
Fragments arranged by size:
1453 1105 850 662 657 432 353 260
139 119 88 80 56
EcoNI CCTnn'n_nnAGG
Cuts at: 410 1871 410
Size: 1461 4793
EcoO109I rG'GnC_Cy
Cuts at: 3393 3393
Size: 6254
EcoRI G'AATT_C
Cuts at: 795 2426 3258 795
Size: 1631 832 3791
Fragments arranged by size:
3791 1631 832
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 476 4273 476
Size: 3797 2457
FspI TGC'GCA
Cuts at: 4035 4035
Size: 6254
HaeII r_GCGC'y
Cuts at: 3688 3936 5166 5937 3688
Size: 248 1230 771 4005
Fragments arranged by size:
4005 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 268 300 594 626 1393 1425 4096 4126 4128
Size: 32 294 32 767 32 2671 30 2
Cuts at: 4128 4158 268
Size: 30 2364
Fragments arranged by size:
2671 2364 767 294 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 3293 3293
Size: 6254
HindIII A'AGCT_T
Cuts at: 711 2574 3111 711
Size: 1863 537 3854
Fragments arranged by size:
3854 1863 537
KasI G'GCGC_C
Cuts at: 3684 3932 3684
Size: 248 6006
KpnI G_GTAC'C
Cuts at: 2905 3274 2905
Size: 369 5885
MluI A'CGCG_T
Cuts at: 5687 5687
Size: 6254
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1403 2346 2796 5013 5197 1403
Size: 943 450 2217 184 2460
Fragments arranged by size:
2460 2217 943 450 184
MscI TGG'CCA
Cuts at: 4015 4015
Size: 6254
MslI CAynn'nnrTG
Cuts at: 892 1433 1641 1952 2171 4243 4370 892
Size: 541 208 311 219 2072 127 2776
Fragments arranged by size:
2776 2072 541 311 219 208 127
MspA1I CmG'CkG
Cuts at: 652 3365 3679 4039 4823 5068 652
Size: 2713 314 360 784 245 1838
Fragments arranged by size:
2713 1838 784 360 314 245
NaeI GCC'GGC
Cuts at: 4435 4435
Size: 6254
NarI GG'CG_CC
Cuts at: 3685 3933 3685
Size: 248 6006
NcoI C'CATG_G
Cuts at: 107 1953 107
Size: 1846 4408
NgoMIV G'CCGG_C
Cuts at: 4433 4433
Size: 6254
NsiI A_TGCA'T
Cuts at: 2549 2549
Size: 6254
NspI r_CATG'y
Cuts at: 663 1148 1644 2547 2751 2975 3250 3307 3807
Size: 485 496 903 204 224 275 57 500
Cuts at: 3807 5410 663
Size: 1603 1507
Fragments arranged by size:
1603 1507 903 500 496 485 275 224
204 57
PasI CC'CwG_GG
Cuts at: 2484 2484
Size: 6254
PciI A'CATG_T
Cuts at: 1144 1640 2971 3246 5406 1144
Size: 496 1331 275 2160 1992
Fragments arranged by size:
2160 1992 1331 496 275
PflMI CCAn_nnn'nTGG
Cuts at: 160 1099 2236 160
Size: 939 1137 4178
Fragments arranged by size:
4178 1137 939
PmeI GTTT'AAAC
Cuts at: 3253 3253
Size: 6254
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4700 4732 4700
Size: 32 6222
PstI C_TGCA'G
Cuts at: 3301 3986 3301
Size: 685 5569
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6254
PvuII CAG'CTG
Cuts at: 652 3679 4039 652
Size: 3027 360 2867
Fragments arranged by size:
3027 2867 360
RsrII CG'GwC_CG
Cuts at: 4449 4449
Size: 6254
SacI G_AGCT'C
Cuts at: 3268 3268
Size: 6254
SalI G'TCGA_C
Cuts at: 3291 3291
Size: 6254
SapI GCTCTTCn'nnn_
Cuts at: 341 2612 4277 4487 341
Size: 2271 1665 210 2108
Fragments arranged by size:
2271 2108 1665 210
SbfI CC_TGCA'GG
Cuts at: 3301 3301
Size: 6254
ScaI AGT'ACT
Cuts at: 944 2441 5771 944
Size: 1497 3330 1427
Fragments arranged by size:
3330 1497 1427
SfcI C'TryA_G
Cuts at: 33 509 1163 1584 1986 2443 3297 3982 4950
Size: 476 654 421 402 457 854 685 968
Cuts at: 4950 5141 33
Size: 191 1146
Fragments arranged by size:
1146 968 854 685 654 476 457 421
402 191
SfoI GGC'GCC
Cuts at: 3686 3934 3686
Size: 248 6006
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6254
SmaI CCC'GGG
Cuts at: 3276 3276
Size: 6254
SmlI C'TyrA_G
Cuts at: 410 1753 1968 2435 2708 3409 4324 5038 5300
Size: 1343 215 467 273 701 915 714 262
Cuts at: 5300 410
Size: 1364
Fragments arranged by size:
1364 1343 915 714 701 467 273 262
215
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6254
SphI G_CATG'C
Cuts at: 663 3307 3807 663
Size: 2644 500 3110
Fragments arranged by size:
3110 2644 500
StuI AGG'CCT
Cuts at: 669 984 669
Size: 315 5939
StyI C'CwwG_G
Cuts at: 107 1466 1953 3136 3388 3742 107
Size: 1359 487 1183 252 354 2619
Fragments arranged by size:
2619 1359 1183 487 354 252
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1856 2497 3837 3970 4429 4638 1856
Size: 641 1340 133 459 209 3472
Fragments arranged by size:
3472 1340 641 459 209 133
TatI w'GTAC_w
Cuts at: 181 942 1537 2439 2668 2791 5769 181
Size: 761 595 902 229 123 2978 666
Fragments arranged by size:
2978 902 761 666 595 229 123
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5771 5803 5771
Size: 32 6222
Tth111I GACn'n_nGTC
Cuts at: 4051 4051
Size: 6254
XbaI T'CTAG_A
Cuts at: 3285 3285
Size: 6254
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1898 1903 3749 1898
Size: 5 1846 4403
Fragments arranged by size:
4403 1846 5
XmaI C'CCGG_G
Cuts at: 3274 3274
Size: 6254
XmnI GAAnn'nnTTC
Cuts at: 588 699 799 2184 588
Size: 111 100 1385 4658
Fragments arranged by size:
4658 1385 111 100
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BfrBI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PasI PciI PflMI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AleI AlfI AloI
ApaI AscI AvrII BcgI BglI BmtI BsgI BsiWI
BssHII BstEII BstZ17I EcoRV FseI FspAI HpaI MfeI
NdeI NheI NotI NruI PacI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI