Restriction map for
pF1KA1132
(Circular) (Six-base) MAPSORT of: pF1KA1132.seq Check: 5263 from: 1 to: 9272
pF1KA1132 9272 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 198 5388 198
Size: 5190 4082
AccI GT'mk_AC
Cuts at: 3257 6310 3257
Size: 3053 6219
Acc65I G'GTAC_C
Cuts at: 284 6288 284
Size: 6004 3268
AclI AA'CG_TT
Cuts at: 9112 9112
Size: 9272
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 182 542 1099 1332 2683 2685 4533 4990 7097
Size: 360 557 233 1351 2 1848 457 2107
Cuts at: 7097 7529 8276 8544 182
Size: 432 747 268 910
Fragments arranged by size:
2107 1848 1351 910 747 557 457 432
360 268 233 2
AfeI AGC'GCT
Cuts at: 4420 6108 4420
Size: 1688 7584
AflIII A'CryG_T
Cuts at: 4190 5261 6624 7728 8705 4190
Size: 1071 1363 1104 977 4757
Fragments arranged by size:
4757 1363 1104 1071 977
AgeI A'CCGG_T
Cuts at: 1556 5333 7660 1556
Size: 3777 2327 3168
Fragments arranged by size:
3777 3168 2327
AleI CACnn'nnGTG
Cuts at: 1389 5041 6262 1389
Size: 3652 1221 4399
Fragments arranged by size:
4399 3652 1221
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2836 3664 4168 2836
Size: 828 504 7940
Fragments arranged by size:
7940 828 504
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2680 2712 2680
Size: 32 9240
AlwNI CAG_nnn'CTG
Cuts at: 769 920 2346 3609 3891 4063 5167 5698 6577
Size: 151 1426 1263 282 172 1104 531 879
Cuts at: 6577 8144 769
Size: 1567 1897
Fragments arranged by size:
1897 1567 1426 1263 1104 879 531 282
172 151
ApaI G_GGCC'C
Cuts at: 1434 3074 3335 3405 5065 5988 6154 1434
Size: 1640 261 70 1660 923 166 4552
Fragments arranged by size:
4552 1660 1640 923 261 166 70
ApaLI G'TGCA_C
Cuts at: 1560 4194 8042 1560
Size: 2634 3848 2790
Fragments arranged by size:
3848 2790 2634
ApoI r'AATT_y
Cuts at: 1915 2053 6276 8625 8881 1915
Size: 138 4223 2349 256 2306
Fragments arranged by size:
4223 2349 2306 256 138
AseI AT'TA_AT
Cuts at: 20 20
Size: 9272
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9272
AvaI C'yCGr_G
Cuts at: 855 1369 1968 2028 3799 5543 6190 6292 855
Size: 514 599 60 1771 1744 647 102 3835
Fragments arranged by size:
3835 1771 1744 647 599 514 102 60
BamHI G'GATC_C
Cuts at: 6297 6297
Size: 9272
BanI G'GyrC_C
Cuts at: 172 240 284 976 1405 1498 2428 3132 3997
Size: 68 44 692 429 93 930 704 865
Cuts at: 3997 4121 5647 6288 6702 6950 6985 172
Size: 124 1526 641 414 248 35 2459
Fragments arranged by size:
2459 1526 930 865 704 692 641 429
414 248 124 93 68 44 35
BanII G_rGCy'C
Cuts at: 262 451 1129 1205 1434 1448 2065 2768 3038
Size: 189 678 76 229 14 617 703 270
Cuts at: 3038 3074 3335 3405 3449 4495 4544 5065 5419
Size: 36 261 70 44 1046 49 521 354
Cuts at: 5419 5864 5988 6154 6175 6286 7316 262
Size: 445 124 166 21 111 1030 2218
Fragments arranged by size:
2218 1046 1030 703 678 617 521 445
354 270 261 229 189 166 124 111
76 70 49 44 36 21 14
BbeI G_GCGC'C
Cuts at: 1409 6706 6954 1409
Size: 5297 248 3727
Fragments arranged by size:
5297 3727 248
BbsI GAAGACnn'nnnn_
Cuts at: 528 1087 4097 528
Size: 559 3010 5703
Fragments arranged by size:
5703 3010 559
BbvCI CC'TCA_GC
Cuts at: 4172 4290 4172
Size: 118 9154
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1674 1708 3334 3368 1674
Size: 34 1626 34 7578
Fragments arranged by size:
7578 1626 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7170 7931 9272 7170
Size: 761 1341 7170
Fragments arranged by size:
7170 1341 761
BclI T'GATC_A
Cuts at: 2015 2477 6792 2015
Size: 462 4315 4495
Fragments arranged by size:
4495 4315 462
BfrBI ATG'CAT
Cuts at: 2114 2114
Size: 9272
BglI GCCn_nnn'nGGC
Cuts at: 6118 6118
Size: 9272
BglII A'GATC_T
Cuts at: 2072 6787 8824 2072
Size: 4715 2037 2520
Fragments arranged by size:
4715 2520 2037
BlpI GC'TnA_GC
Cuts at: 1204 6086 6384 1204
Size: 4882 298 4092
Fragments arranged by size:
4882 4092 298
Bme1580I G_kGCm'C
Cuts at: 245 750 1119 1434 1564 1895 2174 2324 3074
Size: 505 369 315 130 331 279 150 750
Cuts at: 3074 3335 3377 3405 4000 4124 4198 5065 5771
Size: 261 42 28 595 124 74 867 706
Cuts at: 5771 5988 6154 6897 6990 8046 245
Size: 217 166 743 93 1056 1471
Fragments arranged by size:
1471 1056 867 750 743 706 595 505
369 331 315 279 261 217 166 150
130 124 93 74 42 28
BmgBI CAC'GTC
Cuts at: 315 333 1797 2376 3423 5857 315
Size: 18 1464 579 1047 2434 3730
Fragments arranged by size:
3730 2434 1464 1047 579 18
BmrI ACTGGGnnnn_n'
Cuts at: 3282 4794 4927 5140 6484 6565 6900 9044 3282
Size: 1512 133 213 1344 81 335 2144 3510
Fragments arranged by size:
3510 2144 1512 1344 335 213 133 81
BmtI G_CTAG'C
Cuts at: 3722 3722
Size: 9272
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1206 1398 2670 5535 7639 1206
Size: 192 1272 2865 2104 2839
Fragments arranged by size:
2865 2839 2104 1272 192
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2016 2806 3385 4395 4672 5587 6159 2016
Size: 790 579 1010 277 915 572 5129
Fragments arranged by size:
5129 1010 915 790 579 572 277
Bpu10I CC'TnA_GC
Cuts at: 4172 4290 4172
Size: 118 9154
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 871 1336 1768 2074 2653 3259 4426 4838 5325
Size: 465 432 306 579 606 1167 412 487
Cuts at: 5325 6448 7327 7819 8117 871
Size: 1123 879 492 298 2026
Fragments arranged by size:
2026 1167 1123 879 606 579 492 487
465 432 412 306 298
BsaI GGTCTCn'nnnn_
Cuts at: 721 6224 8976 721
Size: 5503 2752 1017
Fragments arranged by size:
5503 2752 1017
BsaAI yAC'GTr
Cuts at: 1878 4193 4454 4742 6625 1878
Size: 2315 261 288 1883 4525
Fragments arranged by size:
4525 2315 1883 288 261
BsaBI GATnn'nnATC
Cuts at: 6 6786 8823 6
Size: 6780 2037 455
Fragments arranged by size:
6780 2037 455
BsaHI Gr'CG_yC
Cuts at: 680 1406 3470 5210 6703 6951 7653 9149 680
Size: 726 2064 1740 1493 248 702 1496 803
Fragments arranged by size:
2064 1740 1496 1493 803 726 702 248
BsaWI w'CCGG_w
Cuts at: 443 1556 3857 5333 5897 6461 6982 7660 7934
Size: 1113 2301 1476 564 564 521 678 274
Cuts at: 7934 8081 8492 443
Size: 147 411 1223
Fragments arranged by size:
2301 1476 1223 1113 678 564 564 521
411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 352 382 589 619 670 700 1813 1843 4567
Size: 30 207 30 51 30 1113 30 2724
Cuts at: 4567 4597 6120 6150 352
Size: 30 1523 30 3474
Fragments arranged by size:
3474 2724 1523 1113 207 51 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 3067 4399 5548 5584 3067
Size: 1332 1149 36 6755
Fragments arranged by size:
6755 1332 1149 36
BseYI C'CCAG_C
Cuts at: 265 1624 1850 2303 2570 2831 3376 3625 3820
Size: 1359 226 453 267 261 545 249 195
Cuts at: 3820 3972 5420 6045 6063 6103 6124 6697 7455
Size: 152 1448 625 18 40 21 573 758
Cuts at: 7455 8032 8528 265
Size: 577 496 1009
Fragments arranged by size:
1448 1359 1009 758 625 577 573 545
496 453 267 261 249 226 195 152
40 21 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 399 583 831 1819 1851 2520 4181 399
Size: 184 248 988 32 669 1661 5490
Fragments arranged by size:
5490 1661 988 669 248 184 32
BsiEI CG_ry'CG
Cuts at: 104 254 3924 6814 6860 8068 104
Size: 150 3670 2890 46 1208 1308
Fragments arranged by size:
3670 2890 1308 1208 150 46
BsiHKAI G_wGCw'C
Cuts at: 1564 2065 2234 3038 3653 4198 5245 5686 6202
Size: 501 169 804 615 545 1047 441 516
Cuts at: 6202 6286 7064 7254 8046 1564
Size: 84 778 190 792 2790
Fragments arranged by size:
2790 1047 804 792 778 615 545 516
501 441 190 169 84
BsiWI C'GTAC_G
Cuts at: 3636 4941 3636
Size: 1305 7967
BsmBI CGTCTCn'nnnn_
Cuts at: 1207 1344 2088 2658 3826 5217 1207
Size: 137 744 570 1168 1391 5262
Fragments arranged by size:
5262 1391 1168 744 570 137
Bsp1286I G_dGCh'C
Cuts at: 245 262 451 750 1119 1129 1205 1434 1448
Size: 17 189 299 369 10 76 229 14
Cuts at: 1448 1564 1895 2065 2174 2234 2324 2768 3038
Size: 116 331 170 109 60 90 444 270
Cuts at: 3038 3074 3335 3377 3405 3449 3653 4000 4124
Size: 36 261 42 28 44 204 347 124
Cuts at: 4124 4198 4495 4544 5065 5245 5419 5686 5771
Size: 74 297 49 521 180 174 267 85
Cuts at: 5771 5864 5988 6154 6175 6202 6286 6897 6990
Size: 93 124 166 21 27 84 611 93
Cuts at: 6990 7064 7254 7316 8046 245
Size: 74 190 62 730 1471
Fragments arranged by size:
1471 730 611 521 444 369 347 331
299 297 270 267 261 229 204 190
189 180 174 170 166 124 124 116
109 93 93 90 85 84 76 74
74 62 60 49 44 42 36 28
27 21 17 14 10
BspEI T'CCGG_A
Cuts at: 443 5897 8492 443
Size: 5454 2595 1223
Fragments arranged by size:
5454 2595 1223
BspHI T'CATG_A
Cuts at: 2057 8448 2057
Size: 6391 2881
BspMI ACCTGCnnnn'nnnn_
Cuts at: 198 2828 4613 5388 5816 6322 6536 6838 7219
Size: 2630 1785 775 428 506 214 302 381
Cuts at: 7219 198
Size: 2251
Fragments arranged by size:
2630 2251 1785 775 506 428 381 302
214
BsrBI CCG'CTC
Cuts at: 50 2294 2553 3061 3558 7564 7618 9102 9267
Size: 2244 259 508 497 4006 54 1484 165
Cuts at: 9267 50
Size: 55
Fragments arranged by size:
4006 2244 1484 508 497 259 165 55
54
BsrDI GCAATG_nn'
Cuts at: 747 1307 4794 7184 747
Size: 560 3487 2390 2835
Fragments arranged by size:
3487 2835 2390 560
BsrFI r'CCGG_y
Cuts at: 433 823 896 1084 1556 3813 3919 4124 4658
Size: 390 73 188 472 2257 106 205 534
Cuts at: 4658 5333 6049 7270 7451 7660 433
Size: 675 716 1221 181 209 2045
Fragments arranged by size:
2257 2045 1221 716 675 534 472 390
209 205 188 181 106 73
BsrGI T'GTAC_A
Cuts at: 653 2978 653
Size: 2325 6947
BssSI C'ACGA_G
Cuts at: 6140 7543 7901 6140
Size: 1403 358 7511
Fragments arranged by size:
7511 1403 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3891 6649 3891
Size: 2758 6514
BstBI TT'CG_AA
Cuts at: 1913 5841 7633 1913
Size: 3928 1792 3552
Fragments arranged by size:
3928 3552 1792
BstEII G'GTnAC_C
Cuts at: 210 993 1422 3120 3945 210
Size: 783 429 1698 825 5537
Fragments arranged by size:
5537 1698 825 783 429
BstXI CCAn_nnnn'nTGG
Cuts at: 114 228 792 2955 3363 4141 4650 114
Size: 114 564 2163 408 778 509 4736
Fragments arranged by size:
4736 2163 778 564 509 408 114
BstYI r'GATC_y
Cuts at: 1 440 1535 2072 2342 2465 2789 4053 6297
Size: 439 1095 537 270 123 324 1264 2244
Cuts at: 6297 6764 6787 7122 7368 8380 8466 8478 8824
Size: 467 23 335 246 1012 86 12 346
Cuts at: 8824 1
Size: 449
Fragments arranged by size:
2244 1264 1095 1012 537 467 449 439
346 335 324 270 246 123 86 23
12
BtgI C'CryG_G
Cuts at: 227 254 419 642 833 1197 1418 2682 3077
Size: 27 165 223 191 364 221 1264 395
Cuts at: 3077 3239 3876 4112 4530 5065 5411 5870 227
Size: 162 637 236 418 535 346 459 3629
Fragments arranged by size:
3629 1264 637 535 459 418 395 364
346 236 223 221 191 165 162 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2100 2487 2607 7222 7403 7566 2100
Size: 387 120 4615 181 163 3806
Fragments arranged by size:
4615 3806 387 181 163 120
BtsI GCAGTG_nn'
Cuts at: 1590 1839 3024 3651 3729 4147 4169 4927 6515
Size: 249 1185 627 78 418 22 758 1588
Cuts at: 6515 1590
Size: 4347
Fragments arranged by size:
4347 1588 1185 758 627 418 249 78
22
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 4194 4229 4194
Size: 35 9237
DraI TTT'AAA
Cuts at: 6271 6271
Size: 9272
DraIII CAC_nnn'GTG
Cuts at: 834 2319 2548 5606 5884 834
Size: 1485 229 3058 278 4222
Fragments arranged by size:
4222 3058 1485 278 229
DrdI GACnn_nn'nnGTC
Cuts at: 2650 3253 3427 6978 7836 2650
Size: 603 174 3551 858 4086
Fragments arranged by size:
4086 3551 858 603 174
EaeI y'GGCC_r
Cuts at: 251 290 530 825 894 1397 1935 2331 2471
Size: 39 240 295 69 503 538 396 140
Cuts at: 2471 3012 3425 3807 3902 3921 4097 4199 4910
Size: 541 413 382 95 19 176 102 711
Cuts at: 4910 5275 6029 6857 7031 7422 7449 8794 251
Size: 365 754 828 174 391 27 1345 729
Fragments arranged by size:
1345 828 754 729 711 541 538 503
413 396 391 382 365 295 240 176
174 140 102 95 69 39 27 19
EagI C'GGCC_G
Cuts at: 251 3921 6857 251
Size: 3670 2936 2666
Fragments arranged by size:
3670 2936 2666
EarI CTCTTCn'nnn_
Cuts at: 561 1234 2378 3342 3856 4573 4939 4945 5526
Size: 673 1144 964 514 717 366 6 581
Cuts at: 5526 7295 7505 561
Size: 1769 210 2328
Fragments arranged by size:
2328 1769 1144 964 717 673 581 514
366 210 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 366 2359 7480 7790 7936 9082 366
Size: 1993 5121 310 146 1146 556
Fragments arranged by size:
5121 1993 1146 556 310 146
EcoICRI GAG'CTC
Cuts at: 2063 3036 6284 2063
Size: 973 3248 5051
Fragments arranged by size:
5051 3248 973
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 182 542 1099 1332 2016 2683 2685 2806 3385
Size: 360 557 233 684 667 2 121 579
Cuts at: 3385 4395 4533 4672 4990 5587 6159 7097 7529
Size: 1010 138 139 318 597 572 938 432
Cuts at: 7529 8276 8544 182
Size: 747 268 910
Fragments arranged by size:
1010 938 910 747 684 667 597 579
572 557 432 360 318 268 233 139
138 121 2
EcoNI CCTnn'n_nnAGG
Cuts at: 2383 2407 5045 5470 2383
Size: 24 2638 425 6185
Fragments arranged by size:
6185 2638 425 24
EcoO109I rG'GnC_Cy
Cuts at: 1253 1431 1528 1778 3071 3331 3332 3402 3682
Size: 178 97 250 1293 260 1 70 280
Cuts at: 3682 3969 4670 4686 5061 5248 5984 5985 6150
Size: 287 701 16 375 187 736 1 165
Cuts at: 6150 6239 6411 1253
Size: 89 172 4114
Fragments arranged by size:
4114 1293 736 701 375 287 280 260
250 187 178 172 165 97 89 70
16 1 1
EcoRI G'AATT_C
Cuts at: 1915 2053 6276 1915
Size: 138 4223 4911
Fragments arranged by size:
4911 4223 138
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 7291 7291
Size: 9272
FseI GG_CCGG'CC
Cuts at: 827 900 827
Size: 73 9199
FspI TGC'GCA
Cuts at: 4756 7053 4756
Size: 2297 6975
HaeII r_GCGC'y
Cuts at: 1409 2154 4422 6110 6706 6954 7976 8955 1409
Size: 745 2268 1688 596 248 1022 979 1726
Fragments arranged by size:
2268 1726 1688 1022 979 745 596 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 496 528 589 621 670 702 2000 2032 5240
Size: 32 61 32 49 32 1298 32 3208
Cuts at: 5240 5272 5611 5643 7114 7144 7146 7176 496
Size: 32 339 32 1471 30 2 30 2592
Fragments arranged by size:
3208 2592 1471 1298 339 61 49 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1365 4236 5187 6311 1365
Size: 2871 951 1124 4326
Fragments arranged by size:
4326 2871 1124 951
HindIII A'AGCT_T
Cuts at: 5028 5028
Size: 9272
KasI G'GCGC_C
Cuts at: 1405 6702 6950 1405
Size: 5297 248 3727
Fragments arranged by size:
5297 3727 248
KpnI G_GTAC'C
Cuts at: 288 6292 288
Size: 6004 3268
MluI A'CGCG_T
Cuts at: 8705 8705
Size: 9272
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1472 2772 5423 7943 8127 1472
Size: 1300 2651 2520 184 2617
Fragments arranged by size:
2651 2617 2520 1300 184
MscI TGG'CCA
Cuts at: 292 3809 4912 6031 7033 292
Size: 3517 1103 1119 1002 2531
Fragments arranged by size:
3517 2531 1119 1103 1002
MslI CAynn'nnrTG
Cuts at: 401 1033 1389 2989 3118 4105 4139 5041 5155
Size: 632 356 1600 129 987 34 902 114
Cuts at: 5155 5260 6163 6262 7261 7388 401
Size: 105 903 99 999 127 2285
Fragments arranged by size:
2285 1600 999 987 903 902 632 356
129 127 114 105 99 34
MspA1I CmG'CkG
Cuts at: 256 421 881 920 974 1148 1941 2570 2677
Size: 165 460 39 54 174 793 629 107
Cuts at: 2677 2705 3878 5097 5577 5992 6383 6697 7057
Size: 28 1173 1219 480 415 391 314 360
Cuts at: 7057 8070 8315 256
Size: 1013 245 1213
Fragments arranged by size:
1219 1213 1173 1013 793 629 480 460
415 391 360 314 245 174 165 107
54 39 28
NaeI GCC'GGC
Cuts at: 435 825 898 3815 3921 6051 7453 435
Size: 390 73 2917 106 2130 1402 2254
Fragments arranged by size:
2917 2254 2130 1402 390 106 73
NarI GG'CG_CC
Cuts at: 1406 6703 6951 1406
Size: 5297 248 3727
Fragments arranged by size:
5297 3727 248
NcoI C'CATG_G
Cuts at: 3077 4112 3077
Size: 1035 8237
NgoMIV G'CCGG_C
Cuts at: 433 823 896 3813 3919 6049 7451 433
Size: 390 73 2917 106 2130 1402 2254
Fragments arranged by size:
2917 2254 2130 1402 390 106 73
NheI G'CTAG_C
Cuts at: 3718 3718
Size: 9272
NsiI A_TGCA'T
Cuts at: 2116 2116
Size: 9272
NspI r_CATG'y
Cuts at: 1980 2114 2284 2994 4594 5265 5560 6325 6825
Size: 134 170 710 1600 671 295 765 500
Cuts at: 6825 7732 1980
Size: 907 3520
Fragments arranged by size:
3520 1600 907 765 710 671 500 295
170 134
PasI CC'CwG_GG
Cuts at: 5058 5087 5980 5058
Size: 29 893 8350
Fragments arranged by size:
8350 893 29
PciI A'CATG_T
Cuts at: 5261 7728 5261
Size: 2467 6805
PflMI CCAn_nnn'nTGG
Cuts at: 834 881 1148 1718 3609 834
Size: 47 267 570 1891 6497
Fragments arranged by size:
6497 1891 570 267 47
PfoI T'CCnGG_A
Cuts at: 134 791 2792 5296 134
Size: 657 2001 2504 4110
Fragments arranged by size:
4110 2504 2001 657
PmeI GTTT'AAAC
Cuts at: 6271 6271
Size: 9272
PmlI CAC'GTG
Cuts at: 4193 4454 4742 4193
Size: 261 288 8723
Fragments arranged by size:
8723 288 261
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5323 5355 8411 8443 5323
Size: 32 3056 32 6152
Fragments arranged by size:
6152 3056 32 32
PpuMI rG'GwC_Cy
Cuts at: 1778 3682 4670 5248 1778
Size: 1904 988 578 5802
Fragments arranged by size:
5802 1904 988 578
PspOMI G'GGCC_C
Cuts at: 1430 3070 3331 3401 5061 5984 6150 1430
Size: 1640 261 70 1660 923 166 4552
Fragments arranged by size:
4552 1660 1640 923 261 166 70
PstI C_TGCA'G
Cuts at: 356 1687 2072 3152 4610 5582 6319 7004 356
Size: 1331 385 1080 1458 972 737 685 2624
Fragments arranged by size:
2624 1458 1331 1080 972 737 685 385
PvuI CG_AT'CG
Cuts at: 104 104
Size: 9272
PvuII CAG'CTG
Cuts at: 1941 2705 5097 5577 6697 7057 1941
Size: 764 2392 480 1120 360 4156
Fragments arranged by size:
4156 2392 1120 764 480 360
RsrII CG'GwC_CG
Cuts at: 962 5745 7467 962
Size: 4783 1722 2767
Fragments arranged by size:
4783 2767 1722
SacI G_AGCT'C
Cuts at: 2065 3038 6286 2065
Size: 973 3248 5051
Fragments arranged by size:
5051 3248 973
SacII CC_GC'GG
Cuts at: 257 422 3879 257
Size: 165 3457 5650
Fragments arranged by size:
5650 3457 165
SalI G'TCGA_C
Cuts at: 6309 6309
Size: 9272
SanDI GG'GwC_CC
Cuts at: 1778 1778
Size: 9272
SapI GCTCTTCn'nnn_
Cuts at: 1234 3856 4939 7295 7505 1234
Size: 2622 1083 2356 210 3001
Fragments arranged by size:
3001 2622 2356 1083 210
SbfI CC_TGCA'GG
Cuts at: 4610 6319 4610
Size: 1709 7563
ScaI AGT'ACT
Cuts at: 4366 8789 4366
Size: 4423 4849
SfcI C'TryA_G
Cuts at: 33 352 1683 2068 3148 3183 3594 4360 4606
Size: 319 1331 385 1080 35 411 766 246
Cuts at: 4606 5454 5578 6315 7000 7993 8184 33
Size: 848 124 737 685 993 191 1121
Fragments arranged by size:
1331 1121 1080 993 848 766 737 685
411 385 319 246 191 124 35
SfoI GGC'GCC
Cuts at: 1407 6704 6952 1407
Size: 5297 248 3727
Fragments arranged by size:
5297 3727 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9272
SmaI CCC'GGG
Cuts at: 1371 3801 5545 6294 1371
Size: 2430 1744 749 4349
Fragments arranged by size:
4349 2430 1744 749
SmlI C'TyrA_G
Cuts at: 886 1315 1783 2089 2668 3274 4441 4853 5340
Size: 429 468 306 579 606 1167 412 487
Cuts at: 5340 6190 6427 7342 7834 8096 886
Size: 850 237 915 492 262 2062
Fragments arranged by size:
2062 1167 915 850 606 579 492 487
468 429 412 306 262 237
SpeI A'CTAG_T
Cuts at: 67 67
Size: 9272
SphI G_CATG'C
Cuts at: 2284 4594 5560 6325 6825 2284
Size: 2310 966 765 500 4731
Fragments arranged by size:
4731 2310 966 765 500
SrfI GCCC'GGGC
Cuts at: 5545 5545
Size: 9272
StuI AGG'CCT
Cuts at: 5371 5833 5890 5371
Size: 462 57 8753
Fragments arranged by size:
8753 462 57
StyI C'CwwG_G
Cuts at: 1400 2264 2285 2630 3077 3665 4012 4079 4112
Size: 864 21 345 447 588 347 67 33
Cuts at: 4112 4664 5963 6406 6760 1400
Size: 552 1299 443 354 3912
Fragments arranged by size:
3912 1299 864 588 552 447 443 354
347 345 67 33 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 979 1638 2277 3150 3634 3888 4588 4854 5240
Size: 659 639 873 484 254 700 266 386
Cuts at: 5240 5742 5938 5993 6108 6855 6988 7447 7656
Size: 502 196 55 115 747 133 459 209
Cuts at: 7656 979
Size: 2595
Fragments arranged by size:
2595 873 747 700 659 639 502 484
459 386 266 254 209 196 133 115
55
TatI w'GTAC_w
Cuts at: 653 1481 2978 2993 3215 3776 4364 5203 8787
Size: 828 1497 15 222 561 588 839 3584
Cuts at: 8787 653
Size: 1138
Fragments arranged by size:
3584 1497 1138 839 828 588 561 222
15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1613 1645 1813 1845 3098 3130 4308 4340 4567
Size: 32 168 32 1253 32 1178 32 227
Cuts at: 4567 4599 6120 6152 8789 8821 1613
Size: 32 1521 32 2637 32 2064
Fragments arranged by size:
2637 2064 1521 1253 1178 227 168 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 601 2587 2605 2647 7069 601
Size: 1986 18 42 4422 2804
Fragments arranged by size:
4422 2804 1986 42 18
XbaI T'CTAG_A
Cuts at: 6303 6303
Size: 9272
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4485 4920 5901 5961 6767 4485
Size: 435 981 60 806 6990
Fragments arranged by size:
6990 981 806 435 60
XhoI C'TCGA_G
Cuts at: 6190 6190
Size: 9272
XmaI C'CCGG_G
Cuts at: 1369 3799 5543 6292 1369
Size: 2430 1744 749 4349
Fragments arranged by size:
4349 2430 1744 749
XmnI GAAnn'nnTTC
Cuts at: 4882 4882
Size: 9272
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SrfI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AscI AvrII BaeI BsmI BssHII
BstZ17I Bsu36I ClaI EcoRV FspAI HpaI MfeI NdeI
NotI NruI PacI PshAI PsiI PspXI PsrI SexAI
SfiI SgrAI SnaBI SspI SwaI ZraI