Restriction map for
pF1KA1139
(Circular) (Six-base) MAPSORT of: pF1KA1139.seq Check: 1202 from: 1 to: 6719
pF1KA1139 6719 bp
With 182 enzymes: SgfI *
September 18, 2009 16:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1291 2190 2874 1291
Size: 899 684 5136
Fragments arranged by size:
5136 899 684
AccI GT'mk_AC
Cuts at: 3757 3757
Size: 6719
Acc65I G'GTAC_C
Cuts at: 3735 3735
Size: 6719
AclI AA'CG_TT
Cuts at: 6559 6559
Size: 6719
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 979 4544 4976 5723 5991 979
Size: 3565 432 747 268 1707
Fragments arranged by size:
3565 1707 747 432 268
AfeI AGC'GCT
Cuts at: 971 971
Size: 6719
AflIII A'CryG_T
Cuts at: 4071 5175 6152 4071
Size: 1104 977 4638
Fragments arranged by size:
4638 1104 977
AgeI A'CCGG_T
Cuts at: 157 5107 157
Size: 4950 1769
AleI CACnn'nnGTG
Cuts at: 623 623
Size: 6719
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 370 488 710 1244 370
Size: 118 222 534 5845
Fragments arranged by size:
5845 534 222 118
AlwNI CAG_nnn'CTG
Cuts at: 126 171 732 936 1425 1452 1563 2914 3073
Size: 45 561 204 489 27 111 1351 159
Cuts at: 3073 3549 4024 5591 126
Size: 476 475 1567 1254
Fragments arranged by size:
1567 1351 1254 561 489 476 475 204
159 111 45 27
ApaI G_GGCC'C
Cuts at: 2459 2697 2792 2813 2882 3529 2459
Size: 238 95 21 69 647 5649
Fragments arranged by size:
5649 647 238 95 69 21
ApaLI G'TGCA_C
Cuts at: 1811 5489 1811
Size: 3678 3041
ApoI r'AATT_y
Cuts at: 577 3723 6072 6328 577
Size: 3146 2349 256 968
Fragments arranged by size:
3146 2349 968 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6719
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6719
AvaI C'yCGr_G
Cuts at: 1122 1271 1924 2657 2933 3020 3223 3739 1122
Size: 149 653 733 276 87 203 516 4102
Fragments arranged by size:
4102 733 653 516 276 203 149 87
AvrII C'CTAG_G
Cuts at: 2574 2574
Size: 6719
BamHI G'GATC_C
Cuts at: 953 3744 953
Size: 2791 3928
BanI G'GyrC_C
Cuts at: 2015 3390 3607 3625 3735 4149 4397 4432 2015
Size: 1375 217 18 110 414 248 35 4302
Fragments arranged by size:
4302 1375 414 248 217 110 35 18
BanII G_rGCy'C
Cuts at: 214 299 1372 2392 2459 2697 2785 2792 2813
Size: 85 1073 1020 67 238 88 7 21
Cuts at: 2813 2855 2882 3230 3529 3733 4763 214
Size: 42 27 348 299 204 1030 2170
Fragments arranged by size:
2170 1073 1030 1020 348 299 238 204
88 85 67 42 27 21 7
BbeI G_GCGC'C
Cuts at: 2019 4153 4401 2019
Size: 2134 248 4337
Fragments arranged by size:
4337 2134 248
BbsI GAAGACnn'nnnn_
Cuts at: 559 559
Size: 6719
BbvCI CC'TCA_GC
Cuts at: 1282 2002 2540 2734 1282
Size: 720 538 194 5267
Fragments arranged by size:
5267 720 538 194
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 985 1019 3657 3691 985
Size: 34 2638 34 4013
Fragments arranged by size:
4013 2638 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4617 5378 6719 4617
Size: 761 1341 4617
Fragments arranged by size:
4617 1341 761
BclI T'GATC_A
Cuts at: 4239 4239
Size: 6719
BglI GCCn_nnn'nGGC
Cuts at: 402 424 1094 2519 2692 2817 402
Size: 22 670 1425 173 125 4304
Fragments arranged by size:
4304 1425 670 173 125 22
BglII A'GATC_T
Cuts at: 1529 4234 6271 1529
Size: 2705 2037 1977
Fragments arranged by size:
2705 2037 1977
BlpI GC'TnA_GC
Cuts at: 1600 1748 1753 3327 3831 1600
Size: 148 5 1574 504 4488
Fragments arranged by size:
4488 1574 504 148 5
Bme1580I G_kGCm'C
Cuts at: 1099 1381 1721 1815 2217 2314 2459 2619 2697
Size: 282 340 94 402 97 145 160 78
Cuts at: 2697 2792 2813 2882 3038 3395 3529 3628 4344
Size: 95 21 69 156 357 134 99 716
Cuts at: 4344 4437 5493 1099
Size: 93 1056 2325
Fragments arranged by size:
2325 1056 716 402 357 340 282 160
156 145 134 99 97 95 94 93
78 69 21
BmgBI CAC'GTC
Cuts at: 846 1545 846
Size: 699 6020
BmrI ACTGGGnnnn_n'
Cuts at: 1825 3481 3524 3931 4012 4347 6491 1825
Size: 1656 43 407 81 335 2144 2053
Fragments arranged by size:
2144 2053 1656 407 335 81 43
BmtI G_CTAG'C
Cuts at: 3466 3466
Size: 6719
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5086 5086
Size: 6719
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 313 412 496 658 850 1457 1585 1636 1819
Size: 99 84 162 192 607 128 51 183
Cuts at: 1819 2080 3185 3275 3571 313
Size: 261 1105 90 296 3461
Fragments arranged by size:
3461 1105 607 296 261 192 183 162
128 99 90 84 51
Bpu10I CC'TnA_GC
Cuts at: 320 1282 1489 2002 2327 2489 2509 2540 2734
Size: 962 207 513 325 162 20 31 194
Cuts at: 2734 3458 320
Size: 724 3581
Fragments arranged by size:
3581 962 724 513 325 207 194 162
31 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 574 958 3370 3895 4774 5266 5564 574
Size: 384 2412 525 879 492 298 1729
Fragments arranged by size:
2412 1729 879 525 492 384 298
BsaI GGTCTCn'nnnn_
Cuts at: 6423 6423
Size: 6719
BsaAI yAC'GTr
Cuts at: 863 4072 863
Size: 3209 3510
BsaBI GATnn'nnATC
Cuts at: 6 748 2047 4233 6270 6
Size: 742 1299 2186 2037 455
Fragments arranged by size:
2186 2037 1299 742 455
BsaHI Gr'CG_yC
Cuts at: 556 2016 2923 3680 3701 4150 4398 5100 6596
Size: 1460 907 757 21 449 248 702 1496
Cuts at: 6596 556
Size: 679
Fragments arranged by size:
1496 1460 907 757 702 679 449 248
21
BsaWI w'CCGG_w
Cuts at: 157 854 2034 2624 3908 4429 5107 5381 5528
Size: 697 1180 590 1284 521 678 274 147
Cuts at: 5528 5939 157
Size: 411 937
Fragments arranged by size:
1284 1180 937 697 678 590 521 411
274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1792 1822 2159 2189 1792
Size: 30 337 30 6322
Fragments arranged by size:
6322 337 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 499 1909 2089 2657 2911 2914 2917 3188 3373
Size: 1410 180 568 254 3 3 271 185
Cuts at: 3373 3485 499
Size: 112 3733
Fragments arranged by size:
3733 1410 568 271 254 185 180 112
3 3
BseYI C'CCAG_C
Cuts at: 599 739 1296 1418 1774 1947 2076 2194 2263
Size: 140 557 122 356 173 129 118 69
Cuts at: 2263 2278 2652 2752 2875 3218 3229 3271 3437
Size: 15 374 100 123 343 11 42 166
Cuts at: 3437 3539 3631 4144 4902 5479 5975 599
Size: 102 92 513 758 577 496 1343
Fragments arranged by size:
1343 758 577 557 513 496 374 356
343 173 166 140 129 123 122 118
102 100 92 69 42 15 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 349 448 458 670 3417 3563 184
Size: 165 99 10 212 2747 146 3340
Fragments arranged by size:
3340 2747 212 165 146 99 10
BsiEI CG_ry'CG
Cuts at: 104 1062 3368 4261 4307 5515 104
Size: 958 2306 893 46 1208 1308
Fragments arranged by size:
2306 1308 1208 958 893 46
BsiHKAI G_wGCw'C
Cuts at: 299 1372 1815 3514 3733 4511 4701 5493 299
Size: 1073 443 1699 219 778 190 792 1525
Fragments arranged by size:
1699 1525 1073 792 778 443 219 190
BsmBI CGTCTCn'nnnn_
Cuts at: 2748 2748
Size: 6719
Bsp1286I G_dGCh'C
Cuts at: 214 299 1099 1372 1381 1721 1815 2217 2314
Size: 85 800 273 9 340 94 402 97
Cuts at: 2314 2392 2459 2619 2697 2785 2792 2813 2855
Size: 78 67 160 78 88 7 21 42
Cuts at: 2855 2882 3038 3230 3395 3514 3529 3628 3733
Size: 27 156 192 165 119 15 99 105
Cuts at: 3733 4344 4437 4511 4701 4763 5493 214
Size: 611 93 74 190 62 730 1440
Fragments arranged by size:
1440 800 730 611 402 340 273 192
190 165 160 156 119 105 99 97
94 93 88 85 78 78 74 67
62 42 27 21 15 9 7
BspEI T'CCGG_A
Cuts at: 854 2034 2624 5939 854
Size: 1180 590 3315 1634
Fragments arranged by size:
3315 1634 1180 590
BspHI T'CATG_A
Cuts at: 5895 5895
Size: 6719
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1291 1802 2190 2844 2874 3769 3983 4285 4666
Size: 511 388 654 30 895 214 302 381
Cuts at: 4666 1291
Size: 3344
Fragments arranged by size:
3344 895 654 511 388 381 302 214
30
BsrBI CCG'CTC
Cuts at: 50 1517 2189 2319 5011 5065 6549 6714 50
Size: 1467 672 130 2692 54 1484 165 55
Fragments arranged by size:
2692 1484 1467 672 165 130 55 54
BsrDI GCAATG_nn'
Cuts at: 354 4631 354
Size: 4277 2442
BsrFI r'CCGG_y
Cuts at: 157 386 1354 1645 2018 2495 4717 4898 5107
Size: 229 968 291 373 477 2222 181 209
Cuts at: 5107 157
Size: 1769
Fragments arranged by size:
2222 1769 968 477 373 291 229 209
181
BssHII G'CGCG_C
Cuts at: 414 414
Size: 6719
BssSI C'ACGA_G
Cuts at: 784 1081 4990 5348 784
Size: 297 3909 358 2155
Fragments arranged by size:
3909 2155 358 297
BstAPI GCAn_nnn'nTGC
Cuts at: 936 4096 936
Size: 3160 3559
BstBI TT'CG_AA
Cuts at: 5080 5080
Size: 6719
BstEII G'GTnAC_C
Cuts at: 1704 1704
Size: 6719
BstXI CCAn_nnnn'nTGG
Cuts at: 1396 1872 1396
Size: 476 6243
BstYI r'GATC_y
Cuts at: 1 455 953 1529 2274 3744 4211 4234 4569
Size: 454 498 576 745 1470 467 23 335
Cuts at: 4569 4815 5827 5913 5925 6271 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1470 1012 745 576 498 467 454 449
346 335 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 948 1682 2915 948
Size: 734 1233 4752
Fragments arranged by size:
4752 1233 734
BtgI C'CryG_G
Cuts at: 107 1389 1865 2092 2144 3499 107
Size: 1282 476 227 52 1355 3327
Fragments arranged by size:
3327 1355 1282 476 227 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1743 4669 4850 5013 1743
Size: 2926 181 163 3449
Fragments arranged by size:
3449 2926 181 163
BtsI GCAGTG_nn'
Cuts at: 631 1001 1329 1356 1423 1625 2619 3109 3121
Size: 370 328 27 67 202 994 490 12
Cuts at: 3121 3169 3962 631
Size: 48 793 3388
Fragments arranged by size:
3388 994 793 490 370 328 202 67
48 27 12
DraI TTT'AAA
Cuts at: 3718 3718
Size: 6719
DraIII CAC_nnn'GTG
Cuts at: 2830 2830
Size: 6719
DrdI GACnn_nn'nnGTC
Cuts at: 3564 4425 5283 3564
Size: 861 858 5000
Fragments arranged by size:
5000 861 858
EaeI y'GGCC_r
Cuts at: 582 705 1059 1386 1505 1549 1694 1877 2024
Size: 123 354 327 119 44 145 183 147
Cuts at: 2024 2045 2141 2232 2349 2450 2602 3140 3365
Size: 21 96 91 117 101 152 538 225
Cuts at: 3365 4304 4478 4869 4896 6241 582
Size: 939 174 391 27 1345 1060
Fragments arranged by size:
1345 1060 939 538 391 354 327 225
183 174 152 147 145 123 119 117
101 96 91 44 27 21
EagI C'GGCC_G
Cuts at: 1059 3365 4304 1059
Size: 2306 939 3474
Fragments arranged by size:
3474 2306 939
EarI CTCTTCn'nnn_
Cuts at: 3592 4742 4952 3592
Size: 1150 210 5359
Fragments arranged by size:
5359 1150 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1981 4927 5237 5383 6529 1981
Size: 2946 310 146 1146 2171
Fragments arranged by size:
2946 2171 1146 310 146
EcoICRI GAG'CTC
Cuts at: 297 1370 3731 297
Size: 1073 2361 3285
Fragments arranged by size:
3285 2361 1073
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 313 412 496 658 850 979 1457 1585 1636
Size: 99 84 162 192 129 478 128 51
Cuts at: 1636 1819 2080 3185 3275 3571 4544 4976 5723
Size: 183 261 1105 90 296 973 432 747
Cuts at: 5723 5991 313
Size: 268 1041
Fragments arranged by size:
1105 1041 973 747 478 432 296 268
261 192 183 162 129 128 99 90
84 51
EcoNI CCTnn'n_nnAGG
Cuts at: 1987 2305 2351 3385 1987
Size: 318 46 1034 5321
Fragments arranged by size:
5321 1034 318 46
EcoO109I rG'GnC_Cy
Cuts at: 123 1686 1980 1998 2356 2456 2707 2788 2809
Size: 1563 294 18 358 100 251 81 21
Cuts at: 2809 2810 2911 3858 123
Size: 1 101 947 2984
Fragments arranged by size:
2984 1563 947 358 294 251 101 100
81 21 18 1
EcoRI G'AATT_C
Cuts at: 3723 3723
Size: 6719
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4738 4738
Size: 6719
FspI TGC'GCA
Cuts at: 4500 4500
Size: 6719
HaeII r_GCGC'y
Cuts at: 781 880 973 2019 2496 2733 2997 3469 3638
Size: 99 93 1046 477 237 264 472 169
Cuts at: 3638 4153 4401 5423 6402 781
Size: 515 248 1022 979 1098
Fragments arranged by size:
1098 1046 1022 979 515 477 472 264
248 237 169 99 93
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 241 273 704 728 736 760 4561 4591 4593
Size: 32 431 24 8 24 3801 30 2
Cuts at: 4593 4623 241
Size: 30 2337
Fragments arranged by size:
3801 2337 431 32 30 30 24 24
8 2
HincII GTy'rAC
Cuts at: 333 540 1917 3758 333
Size: 207 1377 1841 3294
Fragments arranged by size:
3294 1841 1377 207
HindIII A'AGCT_T
Cuts at: 2329 2329
Size: 6719
KasI G'GCGC_C
Cuts at: 2015 4149 4397 2015
Size: 2134 248 4337
Fragments arranged by size:
4337 2134 248
KpnI G_GTAC'C
Cuts at: 3739 3739
Size: 6719
MluI A'CGCG_T
Cuts at: 6152 6152
Size: 6719
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 548 3280 5390 5574 548
Size: 2732 2110 184 1693
Fragments arranged by size:
2732 2110 1693 184
MscI TGG'CCA
Cuts at: 707 1388 1507 2026 2047 2234 2351 3142 4480
Size: 681 119 519 21 187 117 791 1338
Cuts at: 4480 707
Size: 2946
Fragments arranged by size:
2946 1338 791 681 519 187 119 117
21
MslI CAynn'nnrTG
Cuts at: 623 1036 1702 4708 4835 623
Size: 413 666 3006 127 2507
Fragments arranged by size:
3006 2507 666 413 127
MspA1I CmG'CkG
Cuts at: 363 603 1064 1361 1560 1614 1842 1853 2086
Size: 240 461 297 199 54 228 11 233
Cuts at: 2086 2267 3007 3163 3441 3577 3696 3830 4144
Size: 181 740 156 278 136 119 134 314
Cuts at: 4144 4504 5517 5762 363
Size: 360 1013 245 1320
Fragments arranged by size:
1320 1013 740 461 360 314 297 278
245 240 233 228 199 181 156 136
134 119 54 11
NaeI GCC'GGC
Cuts at: 388 1356 1647 2020 4900 388
Size: 968 291 373 2880 2207
Fragments arranged by size:
2880 2207 968 373 291
NarI GG'CG_CC
Cuts at: 2016 4150 4398 2016
Size: 2134 248 4337
Fragments arranged by size:
4337 2134 248
NcoI C'CATG_G
Cuts at: 107 1389 1865 2144 107
Size: 1282 476 279 4682
Fragments arranged by size:
4682 1282 476 279
NgoMIV G'CCGG_C
Cuts at: 386 1354 1645 2018 4898 386
Size: 968 291 373 2880 2207
Fragments arranged by size:
2880 2207 968 373 291
NheI G'CTAG_C
Cuts at: 3462 3462
Size: 6719
NspI r_CATG'y
Cuts at: 357 3772 4272 5179 357
Size: 3415 500 907 1897
Fragments arranged by size:
3415 1897 907 500
PasI CC'CwG_GG
Cuts at: 2385 2410 2432 2785 3307 2385
Size: 25 22 353 522 5797
Fragments arranged by size:
5797 522 353 25 22
PciI A'CATG_T
Cuts at: 5175 5175
Size: 6719
PflMI CCAn_nnn'nTGG
Cuts at: 2422 2776 3187 2422
Size: 354 411 5954
Fragments arranged by size:
5954 411 354
PfoI T'CCnGG_A
Cuts at: 1210 1210
Size: 6719
PmeI GTTT'AAAC
Cuts at: 3718 3718
Size: 6719
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5858 5890 5858
Size: 32 6687
PpuMI rG'GwC_Cy
Cuts at: 123 1686 2356 123
Size: 1563 670 4486
Fragments arranged by size:
4486 1563 670
PspOMI G'GGCC_C
Cuts at: 2455 2693 2788 2809 2878 3525 2455
Size: 238 95 21 69 647 5649
Fragments arranged by size:
5649 647 238 95 69 21
PstI C_TGCA'G
Cuts at: 1167 1643 1904 1991 3116 3766 4451 1167
Size: 476 261 87 1125 650 685 3435
Fragments arranged by size:
3435 1125 685 650 476 261 87
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6719
PvuII CAG'CTG
Cuts at: 603 1560 1614 1842 2086 3441 3577 3696 4144
Size: 957 54 228 244 1355 136 119 448
Cuts at: 4144 4504 603
Size: 360 2818
Fragments arranged by size:
2818 1355 957 448 360 244 228 136
119 54
RsrII CG'GwC_CG
Cuts at: 4914 4914
Size: 6719
SacI G_AGCT'C
Cuts at: 299 1372 3733 299
Size: 1073 2361 3285
Fragments arranged by size:
3285 2361 1073
SalI G'TCGA_C
Cuts at: 3756 3756
Size: 6719
SapI GCTCTTCn'nnn_
Cuts at: 3592 4742 4952 3592
Size: 1150 210 5359
Fragments arranged by size:
5359 1150 210
SbfI CC_TGCA'GG
Cuts at: 3766 3766
Size: 6719
ScaI AGT'ACT
Cuts at: 6236 6236
Size: 6719
SexAI A'CCwGG_T
Cuts at: 1445 1445
Size: 6719
SfcI C'TryA_G
Cuts at: 33 1163 1257 1639 1900 1987 2081 2134 3112
Size: 1130 94 382 261 87 94 53 978
Cuts at: 3112 3762 4447 5440 5631 33
Size: 650 685 993 191 1121
Fragments arranged by size:
1130 1121 993 978 685 650 382 261
191 94 94 87 53
SfoI GGC'GCC
Cuts at: 2017 4151 4399 2017
Size: 2134 248 4337
Fragments arranged by size:
4337 2134 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6719
SmaI CCC'GGG
Cuts at: 1124 3741 1124
Size: 2617 4102
SmlI C'TyrA_G
Cuts at: 589 973 3385 3874 4789 5281 5543 589
Size: 384 2412 489 915 492 262 1765
Fragments arranged by size:
2412 1765 915 492 489 384 262
SnaBI TAC'GTA
Cuts at: 863 863
Size: 6719
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6719
SphI G_CATG'C
Cuts at: 357 3772 4272 357
Size: 3415 500 2804
Fragments arranged by size:
3415 2804 500
StuI AGG'CCT
Cuts at: 946 946
Size: 6719
StyI C'CwwG_G
Cuts at: 107 1389 1865 2027 2144 2352 2574 2792 3246
Size: 1282 476 162 117 208 222 218 454
Cuts at: 3246 3611 3853 4207 107
Size: 365 242 354 2619
Fragments arranged by size:
2619 1282 476 454 365 354 242 222
218 208 162 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 823 1200 1552 3451 4302 4435 4894 5103 823
Size: 377 352 1899 851 133 459 209 2439
Fragments arranged by size:
2439 1899 851 459 377 352 209 133
TatI w'GTAC_w
Cuts at: 6234 6234
Size: 6719
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1456 1488 1792 1824 2159 2191 6236 6268 1456
Size: 32 304 32 335 32 4045 32 1907
Fragments arranged by size:
4045 1907 335 304 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4516 4516
Size: 6719
XbaI T'CTAG_A
Cuts at: 3750 3750
Size: 6719
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 247 531 1872 2055 3567 3651 4214 247
Size: 284 1341 183 1512 84 563 2752
Fragments arranged by size:
2752 1512 1341 563 284 183 84
XmaI C'CCGG_G
Cuts at: 1122 3739 1122
Size: 2617 4102
XmnI GAAnn'nnTTC
Cuts at: 895 895
Size: 6719
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AloI AscI BaeI BfrBI BsiWI
BsmI BsrGI BstZ17I ClaI CspCI EcoRV FseI FspAI
HpaI MfeI NdeI NotI NruI NsiI PacI PmlI
PshAI PsiI PspXI PsrI SacII SanDI SfiI SgrAI
SrfI SspI SwaI XhoI ZraI