Restriction map for
pF1KA1140
(Circular) (Six-base) MAPSORT of: pF1KA1140.seq Check: 3226 from: 1 to: 5330
pF1KA1140 5330 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1469 1572 1469
Size: 103 5227
AccI GT'mk_AC
Cuts at: 2368 2368
Size: 5330
Acc65I G'GTAC_C
Cuts at: 1001 2346 1001
Size: 1345 3985
AclI AA'CG_TT
Cuts at: 5170 5170
Size: 5330
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 526 1288 1447 1867 3155 3587 3940 4602 526
Size: 762 159 420 1288 432 353 662 1254
Fragments arranged by size:
1288 1254 762 662 432 420 353 159
AflIII A'CryG_T
Cuts at: 145 2682 4482 4763 145
Size: 2537 1800 281 712
Fragments arranged by size:
2537 1800 712 281
AhdI GACnn_n'nnGTC
Cuts at: 937 937
Size: 5330
AleI CACnn'nnGTG
Cuts at: 1567 1567
Size: 5330
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1642 1962 1642
Size: 320 5010
AlwNI CAG_nnn'CTG
Cuts at: 720 1311 1329 1587 1647 1765 2205 2635 4073
Size: 591 18 258 60 118 440 430 1438
Cuts at: 4073 720
Size: 1977
Fragments arranged by size:
1977 1438 591 440 430 258 118 60
18
ApaI G_GGCC'C
Cuts at: 1858 1858
Size: 5330
ApaLI G'TGCA_C
Cuts at: 4168 4168
Size: 5330
ApoI r'AATT_y
Cuts at: 988 2334 4683 4939 988
Size: 1346 2349 256 1379
Fragments arranged by size:
2349 1379 1346 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5330
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5330
AvaI C'yCGr_G
Cuts at: 821 2350 821
Size: 1529 3801
BamHI G'GATC_C
Cuts at: 2355 2355
Size: 5330
BanI G'GyrC_C
Cuts at: 843 1001 1332 2346 2760 3008 3043 843
Size: 158 331 1014 414 248 35 3130
Fragments arranged by size:
3130 1014 414 331 248 158 35
BanII G_rGCy'C
Cuts at: 458 542 658 1323 1858 2324 2344 3374 458
Size: 84 116 665 535 466 20 1030 2414
Fragments arranged by size:
2414 1030 665 535 466 116 84 20
BbeI G_GCGC'C
Cuts at: 1336 2764 3012 1336
Size: 1428 248 3654
Fragments arranged by size:
3654 1428 248
BbsI GAAGACnn'nnnn_
Cuts at: 403 403
Size: 5330
BbvCI CC'TCA_GC
Cuts at: 1630 2015 1630
Size: 385 4945
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5296
BciVI GTATCCnnnnn_n'
Cuts at: 3228 4284 5330 3228
Size: 1056 1046 3228
Fragments arranged by size:
3228 1056 1046
BclI T'GATC_A
Cuts at: 338 1175 2850 338
Size: 837 1675 2818
Fragments arranged by size:
2818 1675 837
BfrBI ATG'CAT
Cuts at: 2097 2097
Size: 5330
BglI GCCn_nnn'nGGC
Cuts at: 715 715
Size: 5330
BglII A'GATC_T
Cuts at: 1391 1886 2845 4882 1391
Size: 495 959 2037 1839
Fragments arranged by size:
2037 1839 959 495
BlpI GC'TnA_GC
Cuts at: 835 1322 1904 2442 835
Size: 487 582 538 3723
Fragments arranged by size:
3723 582 538 487
Bme1580I G_kGCm'C
Cuts at: 1103 1858 2955 3048 4172 1103
Size: 755 1097 93 1124 2261
Fragments arranged by size:
2261 1124 1097 755 93
BmgBI CAC'GTC
Cuts at: 416 416
Size: 5330
BmrI ACTGGGnnnn_n'
Cuts at: 1331 2542 2623 2958 5102 1331
Size: 1211 81 335 2144 1559
Fragments arranged by size:
2144 1559 1211 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 375 769 3697 375
Size: 394 2928 2008
Fragments arranged by size:
2928 2008 394
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 466 672 979 1333 1380 1597 1825 2053 2296
Size: 206 307 354 47 217 228 228 243
Cuts at: 2296 466
Size: 3500
Fragments arranged by size:
3500 354 307 243 228 228 217 206
47
Bpu10I CC'TnA_GC
Cuts at: 371 907 1630 2015 371
Size: 536 723 385 3686
Fragments arranged by size:
3686 723 536 385
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1915 2290 2506 3385 4099 4397 1915
Size: 375 216 879 714 298 2848
Fragments arranged by size:
2848 879 714 375 298 216
BsaI GGTCTCn'nnnn_
Cuts at: 559 1237 5034 559
Size: 678 3797 855
Fragments arranged by size:
3797 855 678
BsaAI yAC'GTr
Cuts at: 2183 2683 2183
Size: 500 4830
BsaBI GATnn'nnATC
Cuts at: 6 1174 2844 4881 6
Size: 1168 1670 2037 455
Fragments arranged by size:
2037 1670 1168 455
BsaHI Gr'CG_yC
Cuts at: 1259 1333 2761 3009 3711 5207 1259
Size: 74 1428 248 702 1496 1382
Fragments arranged by size:
1496 1428 1382 702 248 74
BsaWI w'CCGG_w
Cuts at: 863 1292 2519 3040 3718 4129 4276 4550 863
Size: 429 1227 521 678 411 147 274 1643
Fragments arranged by size:
1643 1227 678 521 429 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1642 1672 1753 1783 1801 1831 1642
Size: 30 81 30 18 30 5141
Fragments arranged by size:
5141 81 30 30 30 18
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 343 655 709 784 787 871 1822 2050 343
Size: 312 54 75 3 84 951 228 3623
Fragments arranged by size:
3623 951 312 228 84 75 54 3
BseYI C'CCAG_C
Cuts at: 1661 1665 2124 2755 3513 4178 4586 1661
Size: 4 459 631 758 665 408 2405
Fragments arranged by size:
2405 758 665 631 459 408 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 610 610
Size: 5330
BsiEI CG_ry'CG
Cuts at: 104 2872 2918 4148 104
Size: 2768 46 1230 1286
Fragments arranged by size:
2768 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 458 542 1160 1538 2324 2344 3122 3312 4172
Size: 84 618 378 786 20 778 190 860
Cuts at: 4172 458
Size: 1616
Fragments arranged by size:
1616 860 786 778 618 378 190 84
20
BsmI GAATG_Cn'
Cuts at: 888 888
Size: 5330
Bsp1286I G_dGCh'C
Cuts at: 458 542 658 1103 1160 1323 1538 1858 2324
Size: 84 116 445 57 163 215 320 466
Cuts at: 2324 2344 2955 3048 3122 3312 3374 4172 458
Size: 20 611 93 74 190 62 798 1616
Fragments arranged by size:
1616 798 611 466 445 320 215 190
163 116 93 84 74 62 57 20
BspHI T'CATG_A
Cuts at: 3762 3762
Size: 5330
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1469 1572 1624 1930 2380 2594 2896 3277 1469
Size: 103 52 306 450 214 302 381 3522
Fragments arranged by size:
3522 450 381 306 302 214 103 52
BsrBI CCG'CTC
Cuts at: 50 3622 3676 5160 5325 50
Size: 3572 54 1484 165 55
Fragments arranged by size:
3572 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3242 3242
Size: 5330
BsrFI r'CCGG_y
Cuts at: 2006 3328 3509 2006
Size: 1322 181 3827
Fragments arranged by size:
3827 1322 181
BsrGI T'GTAC_A
Cuts at: 1649 2051 1649
Size: 402 4928
BssSI C'ACGA_G
Cuts at: 1609 2188 3601 4309 1609
Size: 579 1413 708 2630
Fragments arranged by size:
2630 1413 708 579
BstAPI GCAn_nnn'nTGC
Cuts at: 2707 2707
Size: 5330
BstBI TT'CG_AA
Cuts at: 3691 3691
Size: 5330
BstXI CCAn_nnnn'nTGG
Cuts at: 609 1404 609
Size: 795 4535
BstYI r'GATC_y
Cuts at: 1 1391 1886 2355 2822 2845 3180 3426 3732
Size: 1390 495 469 467 23 335 246 306
Cuts at: 3732 3744 3830 4882 1
Size: 12 86 1052 449
Fragments arranged by size:
1390 1052 495 469 467 449 335 306
246 86 23 12
BtgI C'CryG_G
Cuts at: 151 812 1019 1403 2165 151
Size: 661 207 384 762 3316
Fragments arranged by size:
3316 762 661 384 207
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 282 1773 3280 3461 3624 282
Size: 1491 1507 181 163 1988
Fragments arranged by size:
1988 1507 1491 181 163
BtsI GCAGTG_nn'
Cuts at: 1300 1468 2573 1300
Size: 168 1105 4057
Fragments arranged by size:
4057 1105 168
DraI TTT'AAA
Cuts at: 2329 2329
Size: 5330
DrdI GACnn_nn'nnGTC
Cuts at: 1348 3036 4380 1348
Size: 1688 1344 2298
Fragments arranged by size:
2298 1688 1344
EaeI y'GGCC_r
Cuts at: 599 1109 1523 2915 3089 3480 3507 4852 599
Size: 510 414 1392 174 391 27 1345 1077
Fragments arranged by size:
1392 1345 1077 510 414 391 174 27
EagI C'GGCC_G
Cuts at: 2915 2915
Size: 5330
EarI CTCTTCn'nnn_
Cuts at: 1480 1842 1975 3353 3563 1480
Size: 362 133 1378 210 3247
Fragments arranged by size:
3247 1378 362 210 133
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 301 3538 4280 4426 5140 301
Size: 3237 742 146 714 491
Fragments arranged by size:
3237 742 714 491 146
EcoICRI GAG'CTC
Cuts at: 456 540 2322 2342 456
Size: 84 1782 20 3444
Fragments arranged by size:
3444 1782 84 20
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 466 526 672 979 1288 1333 1380 1447 1597
Size: 60 146 307 309 45 47 67 150
Cuts at: 1597 1825 1867 2053 2296 3155 3587 3940 4602
Size: 228 42 186 243 859 432 353 662
Cuts at: 4602 466
Size: 1194
Fragments arranged by size:
1194 859 662 432 353 309 307 243
228 186 150 146 67 60 47 45
42
EcoNI CCTnn'n_nnAGG
Cuts at: 508 637 1186 1210 1678 1849 2035 2209 508
Size: 129 549 24 468 171 186 174 3629
Fragments arranged by size:
3629 549 468 186 174 171 129 24
EcoO109I rG'GnC_Cy
Cuts at: 254 356 618 717 1130 1422 1701 1854 1855
Size: 102 262 99 413 292 279 153 1
Cuts at: 1855 2202 2469 254
Size: 347 267 3115
Fragments arranged by size:
3115 413 347 292 279 267 262 153
102 99 1
EcoRI G'AATT_C
Cuts at: 2334 2334
Size: 5330
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3349 3349
Size: 5330
FspI TGC'GCA
Cuts at: 2089 3111 2089
Size: 1022 4308
FspAI rTGC'GCAy
Cuts at: 2089 2089
Size: 5330
HaeII r_GCGC'y
Cuts at: 491 1336 2065 2764 3012 4242 5013 491
Size: 845 729 699 248 1230 771 808
Fragments arranged by size:
1230 845 808 771 729 699 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 132 164 923 955 1747 1753 1779 1785 1801
Size: 32 759 32 792 6 26 6 16
Cuts at: 1801 1833 2103 2135 3172 3202 3204 3234 132
Size: 32 270 32 1037 30 2 30 2228
Fragments arranged by size:
2228 1037 792 759 270 32 32 32
32 30 30 26 16 6 6 2
HincII GTy'rAC
Cuts at: 1518 2250 2369 1518
Size: 732 119 4479
Fragments arranged by size:
4479 732 119
KasI G'GCGC_C
Cuts at: 1332 2760 3008 1332
Size: 1428 248 3654
Fragments arranged by size:
3654 1428 248
KpnI G_GTAC'C
Cuts at: 1005 2350 1005
Size: 1345 3985
MluI A'CGCG_T
Cuts at: 4763 4763
Size: 5330
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 169 1410 4089 4273 169
Size: 1241 2679 184 1226
Fragments arranged by size:
2679 1241 1226 184
MscI TGG'CCA
Cuts at: 1525 3091 1525
Size: 1566 3764
MslI CAynn'nnrTG
Cuts at: 117 150 737 1171 1499 1567 3319 3446 117
Size: 33 587 434 328 68 1752 127 2001
Fragments arranged by size:
2001 1752 587 434 328 127 68 33
MspA1I CmG'CkG
Cuts at: 1329 1374 1413 1665 1866 2049 2441 2755 3115
Size: 45 39 252 201 183 392 314 360
Cuts at: 3115 3899 4144 1329
Size: 784 245 2515
Fragments arranged by size:
2515 784 392 360 314 252 245 201
183 45 39
NaeI GCC'GGC
Cuts at: 2008 3511 2008
Size: 1503 3827
NarI GG'CG_CC
Cuts at: 1333 2761 3009 1333
Size: 1428 248 3654
Fragments arranged by size:
3654 1428 248
NcoI C'CATG_G
Cuts at: 151 812 1019 1403 151
Size: 661 207 384 4078
Fragments arranged by size:
4078 661 384 207
NgoMIV G'CCGG_C
Cuts at: 2006 3509 2006
Size: 1503 3827
NsiI A_TGCA'T
Cuts at: 2099 2099
Size: 5330
NspI r_CATG'y
Cuts at: 122 149 214 1504 1731 2383 2883 4486 122
Size: 27 65 1290 227 652 500 1603 966
Fragments arranged by size:
1603 1290 966 652 500 227 65 27
PasI CC'CwG_GG
Cuts at: 2226 2226
Size: 5330
PciI A'CATG_T
Cuts at: 145 4482 145
Size: 4337 993
PflMI CCAn_nnn'nTGG
Cuts at: 1464 1464
Size: 5330
PfoI T'CCnGG_A
Cuts at: 361 467 542 1952 361
Size: 106 75 1410 3739
Fragments arranged by size:
3739 1410 106 75
PmeI GTTT'AAAC
Cuts at: 2329 2329
Size: 5330
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3776 3808 3776
Size: 32 5298
PpuMI rG'GwC_Cy
Cuts at: 254 618 1130 254
Size: 364 512 4454
Fragments arranged by size:
4454 512 364
PspOMI G'GGCC_C
Cuts at: 1854 1854
Size: 5330
PstI C_TGCA'G
Cuts at: 262 386 1250 1307 1373 1418 1621 1898 2222
Size: 124 864 57 66 45 203 277 324
Cuts at: 2222 2377 3062 262
Size: 155 685 2530
Fragments arranged by size:
2530 864 685 324 277 203 155 124
66 57 45
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5330
PvuII CAG'CTG
Cuts at: 1329 1374 1413 1665 1866 2049 2755 3115 1329
Size: 45 39 252 201 183 706 360 3544
Fragments arranged by size:
3544 706 360 252 201 183 45 39
RsrII CG'GwC_CG
Cuts at: 3525 3525
Size: 5330
SacI G_AGCT'C
Cuts at: 458 542 2324 2344 458
Size: 84 1782 20 3444
Fragments arranged by size:
3444 1782 84 20
SalI G'TCGA_C
Cuts at: 2367 2367
Size: 5330
SanDI GG'GwC_CC
Cuts at: 1130 1130
Size: 5330
SapI GCTCTTCn'nnn_
Cuts at: 1480 3353 3563 1480
Size: 1873 210 3247
Fragments arranged by size:
3247 1873 210
SbfI CC_TGCA'GG
Cuts at: 262 386 1250 2377 262
Size: 124 864 1127 3215
Fragments arranged by size:
3215 1127 864 124
ScaI AGT'ACT
Cuts at: 1991 4847 1991
Size: 2856 2474
SexAI A'CCwGG_T
Cuts at: 1004 1004
Size: 5330
SfcI C'TryA_G
Cuts at: 33 258 382 1239 1246 1303 1369 1414 1617
Size: 225 124 857 7 57 66 45 203
Cuts at: 1617 1894 2218 2373 3058 4026 4217 33
Size: 277 324 155 685 968 191 1146
Fragments arranged by size:
1146 968 857 685 324 277 225 203
191 155 124 66 57 45 7
SfoI GGC'GCC
Cuts at: 1334 2762 3010 1334
Size: 1428 248 3654
Fragments arranged by size:
3654 1428 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5330
SmaI CCC'GGG
Cuts at: 2352 2352
Size: 5330
SmlI C'TyrA_G
Cuts at: 821 1930 2269 2485 3400 4114 4376 821
Size: 1109 339 216 915 714 262 1775
Fragments arranged by size:
1775 1109 915 714 339 262 216
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5330
SphI G_CATG'C
Cuts at: 214 1504 1731 2383 2883 214
Size: 1290 227 652 500 2661
Fragments arranged by size:
2661 1290 652 500 227
StuI AGG'CCT
Cuts at: 269 1673 269
Size: 1404 3926
StyI C'CwwG_G
Cuts at: 151 764 812 1019 1211 1403 1565 2464 2818
Size: 613 48 207 192 192 162 899 354
Cuts at: 2818 151
Size: 2663
Fragments arranged by size:
2663 899 613 354 207 192 192 162
48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1830 2913 3046 3505 3714 1830
Size: 1083 133 459 209 3446
Fragments arranged by size:
3446 1083 459 209 133
TatI w'GTAC_w
Cuts at: 1649 1989 2051 2292 4845 1649
Size: 340 62 241 2553 2134
Fragments arranged by size:
2553 2134 340 241 62
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4847 4879 4847
Size: 32 5298
Tth111I GACn'n_nGTC
Cuts at: 3127 3127
Size: 5330
XbaI T'CTAG_A
Cuts at: 2361 2361
Size: 5330
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1218 1572 2825 1218
Size: 354 1253 3723
Fragments arranged by size:
3723 1253 354
XhoI C'TCGA_G
Cuts at: 821 821
Size: 5330
XmaI C'CCGG_G
Cuts at: 2350 2350
Size: 5330
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AloI AscI AvrII BaeI
BmtI BsiWI BsmBI BspEI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI HindIII HpaI MfeI
NdeI NheI NotI NruI PacI PmlI PshAI PsiI
PspXI PsrI SacII SfiI SgrAI SnaBI SrfI SspI
SwaI XmnI ZraI