Restriction map for
pF1KA1150
(Circular) (Six-base) MAPSORT of: pF1KA1150.seq Check: 8310 from: 1 to: 4892
pF1KA1150 4892 bp
With 182 enzymes: SgfI *
January 9, 2009 11:10 ..
AccI GT'mk_AC
Cuts at: 1930 1930
Size: 4892
Acc65I G'GTAC_C
Cuts at: 1908 1908
Size: 4892
AclI AA'CG_TT
Cuts at: 4732 4732
Size: 4892
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2717 3149 3502 4164 2717
Size: 432 353 662 3445
Fragments arranged by size:
3445 662 432 353
AflIII A'CryG_T
Cuts at: 881 897 2244 4044 4325 881
Size: 16 1347 1800 281 1448
Fragments arranged by size:
1800 1448 1347 281 16
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1087 1119 1087
Size: 32 4860
AlwNI CAG_nnn'CTG
Cuts at: 2197 3635 2197
Size: 1438 3454
ApaI G_GGCC'C
Cuts at: 801 943 801
Size: 142 4750
ApaLI G'TGCA_C
Cuts at: 3730 3730
Size: 4892
ApoI r'AATT_y
Cuts at: 819 1896 4245 4501 819
Size: 1077 2349 256 1210
Fragments arranged by size:
2349 1210 1077 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4892
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4892
AvaI C'yCGr_G
Cuts at: 1912 1912
Size: 4892
BamHI G'GATC_C
Cuts at: 1917 1917
Size: 4892
BanI G'GyrC_C
Cuts at: 282 1713 1908 2322 2570 2605 282
Size: 1431 195 414 248 35 2569
Fragments arranged by size:
2569 1431 414 248 195 35
BanII G_rGCy'C
Cuts at: 801 943 1906 2936 801
Size: 142 963 1030 2757
Fragments arranged by size:
2757 1030 963 142
BbeI G_GCGC'C
Cuts at: 2326 2574 2326
Size: 248 4644
BbsI GAAGACnn'nnnn_
Cuts at: 709 709
Size: 4892
BbvCI CC'TCA_GC
Cuts at: 613 613
Size: 4892
BciVI GTATCCnnnnn_n'
Cuts at: 2790 3846 4892 2790
Size: 1056 1046 2790
Fragments arranged by size:
2790 1056 1046
BclI T'GATC_A
Cuts at: 2412 2412
Size: 4892
BfrBI ATG'CAT
Cuts at: 1498 1498
Size: 4892
BglI GCCn_nnn'nGGC
Cuts at: 1168 1168
Size: 4892
BglII A'GATC_T
Cuts at: 2407 4444 2407
Size: 2037 2855
BlpI GC'TnA_GC
Cuts at: 2004 2004
Size: 4892
Bme1580I G_kGCm'C
Cuts at: 801 943 1371 2517 2610 3734 801
Size: 142 428 1146 93 1124 1959
Fragments arranged by size:
1959 1146 1124 428 142 93
BmrI ACTGGGnnnn_n'
Cuts at: 1365 1713 1799 2104 2185 2520 4664 1365
Size: 348 86 305 81 335 2144 1593
Fragments arranged by size:
2144 1593 348 335 305 86 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3259 3259
Size: 4892
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 692 1222 1562 1645 692
Size: 530 340 83 3939
Fragments arranged by size:
3939 530 340 83
Bpu10I CC'TnA_GC
Cuts at: 613 947 613
Size: 334 4558
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 2068 2947 3661 3959 298
Size: 1770 879 714 298 1231
Fragments arranged by size:
1770 1231 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1600 4596 1600
Size: 2996 1896
BsaAI yAC'GTr
Cuts at: 2245 2245
Size: 4892
BsaBI GATnn'nnATC
Cuts at: 6 2406 4443 6
Size: 2400 2037 455
Fragments arranged by size:
2400 2037 455
BsaHI Gr'CG_yC
Cuts at: 2323 2571 3273 4769 2323
Size: 248 702 1496 2446
Fragments arranged by size:
2446 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2081 2602 3280 3691 3838 4112 2081
Size: 521 678 411 147 274 2861
Fragments arranged by size:
2861 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1560 1590 1560
Size: 30 4862
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 601 601
Size: 4892
BseYI C'CCAG_C
Cuts at: 165 915 920 1123 2317 3075 3740 4148 165
Size: 750 5 203 1194 758 665 408 909
Fragments arranged by size:
1194 909 758 750 665 408 203 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1274 1274
Size: 4892
BsiEI CG_ry'CG
Cuts at: 104 2434 2480 3710 104
Size: 2330 46 1230 1286
Fragments arranged by size:
2330 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1906 2684 2874 3734 1906
Size: 778 190 860 3064
Fragments arranged by size:
3064 860 778 190
BsmI GAATG_Cn'
Cuts at: 726 726
Size: 4892
Bsp1286I G_dGCh'C
Cuts at: 801 943 1371 1906 2517 2610 2684 2874 2936
Size: 142 428 535 611 93 74 190 62
Cuts at: 2936 3734 801
Size: 798 1959
Fragments arranged by size:
1959 798 611 535 428 190 142 93
74 62
BspHI T'CATG_A
Cuts at: 1613 3324 1613
Size: 1711 3181
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1942 2156 2458 2839 1942
Size: 214 302 381 3995
Fragments arranged by size:
3995 381 302 214
BsrBI CCG'CTC
Cuts at: 50 591 1688 3184 3238 4722 4887 50
Size: 541 1097 1496 54 1484 165 55
Fragments arranged by size:
1496 1484 1097 541 165 55 54
BsrDI GCAATG_nn'
Cuts at: 247 1763 2804 247
Size: 1516 1041 2335
Fragments arranged by size:
2335 1516 1041
BsrFI r'CCGG_y
Cuts at: 1484 2890 3071 1484
Size: 1406 181 3305
Fragments arranged by size:
3305 1406 181
BsrGI T'GTAC_A
Cuts at: 714 714
Size: 4892
BssSI C'ACGA_G
Cuts at: 3163 3871 3163
Size: 708 4184
BstAPI GCAn_nnn'nTGC
Cuts at: 2269 2269
Size: 4892
BstBI TT'CG_AA
Cuts at: 3253 3253
Size: 4892
BstYI r'GATC_y
Cuts at: 1 1205 1917 2384 2407 2742 2988 3294 3306
Size: 1204 712 467 23 335 246 306 12
Cuts at: 3306 3392 4444 1
Size: 86 1052 449
Fragments arranged by size:
1204 1052 712 467 449 335 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 224 107
Size: 117 4775
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2842 3023 3186 2842
Size: 181 163 4548
Fragments arranged by size:
4548 181 163
BtsI GCAGTG_nn'
Cuts at: 321 1441 2135 321
Size: 1120 694 3078
Fragments arranged by size:
3078 1120 694
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 866 901 866
Size: 35 4857
DraI TTT'AAA
Cuts at: 1891 1891
Size: 4892
DrdI GACnn_nn'nnGTC
Cuts at: 2598 3942 2598
Size: 1344 3548
EaeI y'GGCC_r
Cuts at: 2477 2651 3042 3069 4414 2477
Size: 174 391 27 1345 2955
Fragments arranged by size:
2955 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2477 2477
Size: 4892
EarI CTCTTCn'nnn_
Cuts at: 137 1178 2915 3125 137
Size: 1041 1737 210 1904
Fragments arranged by size:
1904 1737 1041 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3100 3842 3988 4702 3100
Size: 742 146 714 3290
Fragments arranged by size:
3290 742 714 146
EcoICRI GAG'CTC
Cuts at: 1904 1904
Size: 4892
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 692 1222 1562 1645 2717 3149 3502 4164 692
Size: 530 340 83 1072 432 353 662 1420
Fragments arranged by size:
1420 1072 662 530 432 353 340 83
EcoNI CCTnn'n_nnAGG
Cuts at: 1516 1516
Size: 4892
EcoO109I rG'GnC_Cy
Cuts at: 768 797 939 1801 2031 768
Size: 29 142 862 230 3629
Fragments arranged by size:
3629 862 230 142 29
EcoRI G'AATT_C
Cuts at: 1896 1896
Size: 4892
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 148 174 358 2911 148
Size: 26 184 2553 2129
Fragments arranged by size:
2553 2129 184 26
FspI TGC'GCA
Cuts at: 2673 2673
Size: 4892
HaeII r_GCGC'y
Cuts at: 1130 2326 2574 3804 4575 1130
Size: 1196 248 1230 771 1447
Fragments arranged by size:
1447 1230 1196 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 170 202 657 689 2734 2764 2766 2796 170
Size: 32 455 32 2045 30 2 30 2266
Fragments arranged by size:
2266 2045 455 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1761 1931 1761
Size: 170 4722
HindIII A'AGCT_T
Cuts at: 778 778
Size: 4892
KasI G'GCGC_C
Cuts at: 2322 2570 2322
Size: 248 4644
KpnI G_GTAC'C
Cuts at: 1912 1912
Size: 4892
MluI A'CGCG_T
Cuts at: 4325 4325
Size: 4892
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1472 3651 3835 1472
Size: 2179 184 2529
Fragments arranged by size:
2529 2179 184
MscI TGG'CCA
Cuts at: 2653 2653
Size: 4892
MslI CAynn'nnrTG
Cuts at: 886 1057 1276 2881 3008 886
Size: 171 219 1605 127 2770
Fragments arranged by size:
2770 1605 219 171 127
MspA1I CmG'CkG
Cuts at: 612 936 1185 1582 2003 2317 2677 3461 3706
Size: 324 249 397 421 314 360 784 245
Cuts at: 3706 612
Size: 1798
Fragments arranged by size:
1798 784 421 397 360 324 314 249
245
NaeI GCC'GGC
Cuts at: 3073 3073
Size: 4892
NarI GG'CG_CC
Cuts at: 2323 2571 2323
Size: 248 4644
NcoI C'CATG_G
Cuts at: 107 224 107
Size: 117 4775
NgoMIV G'CCGG_C
Cuts at: 3071 3071
Size: 4892
NsiI A_TGCA'T
Cuts at: 1500 1500
Size: 4892
NspI r_CATG'y
Cuts at: 885 1945 2445 4048 885
Size: 1060 500 1603 1729
Fragments arranged by size:
1729 1603 1060 500
PciI A'CATG_T
Cuts at: 881 4044 881
Size: 3163 1729
PfoI T'CCnGG_A
Cuts at: 1199 1199
Size: 4892
PmeI GTTT'AAAC
Cuts at: 1891 1891
Size: 4892
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3338 3370 3338
Size: 32 4860
PspOMI G'GGCC_C
Cuts at: 797 939 797
Size: 142 4750
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1526 1558 1526
Size: 32 4860
PstI C_TGCA'G
Cuts at: 1161 1939 2624 1161
Size: 778 685 3429
Fragments arranged by size:
3429 778 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4892
PvuII CAG'CTG
Cuts at: 612 1185 1582 2317 2677 612
Size: 573 397 735 360 2827
Fragments arranged by size:
2827 735 573 397 360
RsrII CG'GwC_CG
Cuts at: 3087 3087
Size: 4892
SacI G_AGCT'C
Cuts at: 1906 1906
Size: 4892
SalI G'TCGA_C
Cuts at: 1929 1929
Size: 4892
SapI GCTCTTCn'nnn_
Cuts at: 137 1178 2915 3125 137
Size: 1041 1737 210 1904
Fragments arranged by size:
1904 1737 1041 210
SbfI CC_TGCA'GG
Cuts at: 1939 1939
Size: 4892
ScaI AGT'ACT
Cuts at: 4409 4409
Size: 4892
SfcI C'TryA_G
Cuts at: 33 679 925 1157 1513 1935 2620 3588 3779
Size: 646 246 232 356 422 685 968 191
Cuts at: 3779 33
Size: 1146
Fragments arranged by size:
1146 968 685 646 422 356 246 232
191
SfoI GGC'GCC
Cuts at: 2324 2572 2324
Size: 248 4644
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4892
SmaI CCC'GGG
Cuts at: 1914 1914
Size: 4892
SmlI C'TyrA_G
Cuts at: 277 2047 2962 3676 3938 277
Size: 1770 915 714 262 1231
Fragments arranged by size:
1770 1231 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4892
SphI G_CATG'C
Cuts at: 1945 2445 1945
Size: 500 4392
StuI AGG'CCT
Cuts at: 360 360
Size: 4892
StyI C'CwwG_G
Cuts at: 107 224 801 1320 1743 2026 2380 107
Size: 117 577 519 423 283 354 2619
Fragments arranged by size:
2619 577 519 423 354 283 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 990 1832 2475 2608 3067 3276 990
Size: 842 643 133 459 209 2606
Fragments arranged by size:
2606 842 643 459 209 133
TatI w'GTAC_w
Cuts at: 714 4407 714
Size: 3693 1199
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1560 1592 4409 4441 1560
Size: 32 2817 32 2011
Fragments arranged by size:
2817 2011 32 32
Tth111I GACn'n_nGTC
Cuts at: 671 2689 671
Size: 2018 2874
XbaI T'CTAG_A
Cuts at: 1923 1923
Size: 4892
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 528 2387 528
Size: 1859 3033
XmaI C'CCGG_G
Cuts at: 1912 1912
Size: 4892
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PciI PfoI PmeI PpiI PspOMI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AscI AvrII BaeI BcgI BmgBI BmtI BsiWI BsmBI
BspEI BssHII BstEII BstXI BstZ17I Bsu36I ClaI DraIII
EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI
NruI PacI PasI PflMI PmlI PpuMI PshAI PsiI
PspXI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI