Restriction map for
pF1KA1154
(Circular) (Six-base) MAPSORT of: pF1KA1154.seq Check: 6719 from: 1 to: 4541
pF1KA1154 4541 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AccI GT'mk_AC
Cuts at: 1148 1442 1579 1148
Size: 294 137 4110
Fragments arranged by size:
4110 294 137
Acc65I G'GTAC_C
Cuts at: 1557 1557
Size: 4541
AclI AA'CG_TT
Cuts at: 4381 4381
Size: 4541
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 496 949 2366 2798 3545 3813 271
Size: 225 453 1417 432 747 268 999
Fragments arranged by size:
1417 999 747 453 432 268 225
AflIII A'CryG_T
Cuts at: 485 1893 2997 3974 485
Size: 1408 1104 977 1052
Fragments arranged by size:
1408 1104 1052 977
AgeI A'CCGG_T
Cuts at: 2929 2929
Size: 4541
AlwNI CAG_nnn'CTG
Cuts at: 927 1846 3413 927
Size: 919 1567 2055
Fragments arranged by size:
2055 1567 919
ApaLI G'TGCA_C
Cuts at: 522 3311 522
Size: 2789 1752
ApoI r'AATT_y
Cuts at: 957 1002 1545 3894 4150 957
Size: 45 543 2349 256 1348
Fragments arranged by size:
2349 1348 543 256 45
AseI AT'TA_AT
Cuts at: 20 20
Size: 4541
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4541
AvaI C'yCGr_G
Cuts at: 1561 1561
Size: 4541
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1094 1127 1094
Size: 33 4508
BamHI G'GATC_C
Cuts at: 844 898 1566 844
Size: 54 668 3819
Fragments arranged by size:
3819 668 54
BanI G'GyrC_C
Cuts at: 200 276 1327 1557 1971 2219 2254 200
Size: 76 1051 230 414 248 35 2487
Fragments arranged by size:
2487 1051 414 248 230 76 35
BanII G_rGCy'C
Cuts at: 1555 2585 1555
Size: 1030 3511
BbeI G_GCGC'C
Cuts at: 204 1975 2223 204
Size: 1771 248 2522
Fragments arranged by size:
2522 1771 248
BbsI GAAGACnn'nnnn_
Cuts at: 237 956 1205 237
Size: 719 249 3573
Fragments arranged by size:
3573 719 249
BbvCI CC'TCA_GC
Cuts at: 920 920
Size: 4541
BciVI GTATCCnnnnn_n'
Cuts at: 2439 3200 4541 2439
Size: 761 1341 2439
Fragments arranged by size:
2439 1341 761
BclI T'GATC_A
Cuts at: 2061 2061
Size: 4541
BglII A'GATC_T
Cuts at: 2056 4093 2056
Size: 2037 2504
BlpI GC'TnA_GC
Cuts at: 1653 1653
Size: 4541
Bme1580I G_kGCm'C
Cuts at: 526 2166 2259 3315 526
Size: 1640 93 1056 1752
Fragments arranged by size:
1752 1640 1056 93
BmgBI CAC'GTC
Cuts at: 954 954
Size: 4541
BmrI ACTGGGnnnn_n'
Cuts at: 1753 1834 2169 4313 1753
Size: 81 335 2144 1981
Fragments arranged by size:
2144 1981 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2908 2908
Size: 4541
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 392 1070 392
Size: 678 3863
Bpu10I CC'TnA_GC
Cuts at: 920 1262 920
Size: 342 4199
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1391 1717 2596 3088 3386 1391
Size: 326 879 492 298 2546
Fragments arranged by size:
2546 879 492 326 298
BsaI GGTCTCn'nnnn_
Cuts at: 542 4245 542
Size: 3703 838
BsaAI yAC'GTr
Cuts at: 366 1894 366
Size: 1528 3013
BsaBI GATnn'nnATC
Cuts at: 6 2055 4092 6
Size: 2049 2037 455
Fragments arranged by size:
2049 2037 455
BsaHI Gr'CG_yC
Cuts at: 201 1972 2220 2922 4418 201
Size: 1771 248 702 1496 324
Fragments arranged by size:
1771 1496 702 324 248
BsaWI w'CCGG_w
Cuts at: 329 1730 2251 2929 3203 3350 3761 329
Size: 1401 521 678 274 147 411 1109
Fragments arranged by size:
1401 1109 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 660 908 1288 1351 1363 660
Size: 248 380 63 12 3838
Fragments arranged by size:
3838 380 248 63 12
BseYI C'CCAG_C
Cuts at: 561 1966 2724 3301 3797 561
Size: 1405 758 577 496 1305
Fragments arranged by size:
1405 1305 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2083 2129 3337 104
Size: 1979 46 1208 1308
Fragments arranged by size:
1979 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 526 1555 2333 2523 3315 526
Size: 1029 778 190 792 1752
Fragments arranged by size:
1752 1029 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 526 1555 2166 2259 2333 2523 2585 3315 526
Size: 1029 611 93 74 190 62 730 1752
Fragments arranged by size:
1752 1029 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 329 3761 329
Size: 3432 1109
BspHI T'CATG_A
Cuts at: 3717 3717
Size: 4541
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1350 1591 1805 2107 2488 1350
Size: 241 214 302 381 3403
Fragments arranged by size:
3403 381 302 241 214
BsrBI CCG'CTC
Cuts at: 50 113 353 650 2833 2887 4371 4536 50
Size: 63 240 297 2183 54 1484 165 55
Fragments arranged by size:
2183 1484 297 240 165 63 55 54
BsrDI GCAATG_nn'
Cuts at: 2453 2453
Size: 4541
BsrFI r'CCGG_y
Cuts at: 262 2539 2720 2929 262
Size: 2277 181 209 1874
Fragments arranged by size:
2277 1874 209 181
BssSI C'ACGA_G
Cuts at: 1313 2812 3170 1313
Size: 1499 358 2684
Fragments arranged by size:
2684 1499 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1309 1918 1309
Size: 609 3932
BstBI TT'CG_AA
Cuts at: 239 2902 239
Size: 2663 1878
BstEII G'GTnAC_C
Cuts at: 258 1219 258
Size: 961 3580
BstYI r'GATC_y
Cuts at: 1 344 844 898 1566 2033 2056 2391 2637
Size: 343 500 54 668 467 23 335 246
Cuts at: 2637 3649 3735 3747 4093 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 668 500 467 449 346 343 335
246 86 54 23 12
BtgI C'CryG_G
Cuts at: 310 1529 310
Size: 1219 3322
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2491 2672 2835 2491
Size: 181 163 4197
Fragments arranged by size:
4197 181 163
BtsI GCAGTG_nn'
Cuts at: 1425 1784 1425
Size: 359 4182
DraI TTT'AAA
Cuts at: 1540 1540
Size: 4541
DrdI GACnn_nn'nnGTC
Cuts at: 2247 3105 2247
Size: 858 3683
EaeI y'GGCC_r
Cuts at: 318 2126 2300 2691 2718 4063 318
Size: 1808 174 391 27 1345 796
Fragments arranged by size:
1808 1345 796 391 174 27
EagI C'GGCC_G
Cuts at: 2126 2126
Size: 4541
EarI CTCTTCn'nnn_
Cuts at: 151 925 1223 2564 2774 151
Size: 774 298 1341 210 1918
Fragments arranged by size:
1918 1341 774 298 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2749 3059 3205 4351 2749
Size: 310 146 1146 2939
Fragments arranged by size:
2939 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1553 1553
Size: 4541
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 392 496 949 1070 2366 2798 3545 3813
Size: 121 104 453 121 1296 432 747 268
Cuts at: 3813 271
Size: 999
Fragments arranged by size:
1296 999 747 453 432 268 121 121
104
EcoNI CCTnn'n_nnAGG
Cuts at: 253 839 1228 1390 253
Size: 586 389 162 3404
Fragments arranged by size:
3404 586 389 162
EcoO109I rG'GnC_Cy
Cuts at: 183 1680 183
Size: 1497 3044
EcoRI G'AATT_C
Cuts at: 957 1002 1545 957
Size: 45 543 3953
Fragments arranged by size:
3953 543 45
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 703 2560 703
Size: 1857 2684
FspI TGC'GCA
Cuts at: 2322 2322
Size: 4541
HaeII r_GCGC'y
Cuts at: 125 204 1975 2223 3245 4224 125
Size: 79 1771 248 1022 979 442
Fragments arranged by size:
1771 1022 979 442 248 79
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 865 897 898 930 1269 1301 2383 2413 2415
Size: 32 1 32 339 32 1082 30 2
Cuts at: 2415 2445 865
Size: 30 2961
Fragments arranged by size:
2961 1082 339 32 32 32 30 30
2 1
HincII GTy'rAC
Cuts at: 791 890 1580 791
Size: 99 690 3752
Fragments arranged by size:
3752 690 99
HindIII A'AGCT_T
Cuts at: 818 818
Size: 4541
KasI G'GCGC_C
Cuts at: 200 1971 2219 200
Size: 1771 248 2522
Fragments arranged by size:
2522 1771 248
KpnI G_GTAC'C
Cuts at: 1561 1561
Size: 4541
MluI A'CGCG_T
Cuts at: 3974 3974
Size: 4541
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 689 876 3212 3396 689
Size: 187 2336 184 1834
Fragments arranged by size:
2336 1834 187 184
MscI TGG'CCA
Cuts at: 320 2302 320
Size: 1982 2559
MslI CAynn'nnrTG
Cuts at: 2530 2657 2530
Size: 127 4414
MspA1I CmG'CkG
Cuts at: 312 1652 1966 2326 3339 3584 312
Size: 1340 314 360 1013 245 1269
Fragments arranged by size:
1340 1269 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2722 2722
Size: 4541
NarI GG'CG_CC
Cuts at: 201 1972 2220 201
Size: 1771 248 2522
Fragments arranged by size:
2522 1771 248
NgoMIV G'CCGG_C
Cuts at: 2720 2720
Size: 4541
NspI r_CATG'y
Cuts at: 1594 2094 3001 1594
Size: 500 907 3134
Fragments arranged by size:
3134 907 500
PciI A'CATG_T
Cuts at: 2997 2997
Size: 4541
PmeI GTTT'AAAC
Cuts at: 1540 1540
Size: 4541
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3680 3712 3680
Size: 32 4509
PpuMI rG'GwC_Cy
Cuts at: 183 183
Size: 4541
PstI C_TGCA'G
Cuts at: 1358 1588 2273 1358
Size: 230 685 3626
Fragments arranged by size:
3626 685 230
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4541
PvuII CAG'CTG
Cuts at: 1966 2326 1966
Size: 360 4181
RsrII CG'GwC_CG
Cuts at: 2736 2736
Size: 4541
SacI G_AGCT'C
Cuts at: 1555 1555
Size: 4541
SacII CC_GC'GG
Cuts at: 313 313
Size: 4541
SalI G'TCGA_C
Cuts at: 1578 1578
Size: 4541
SanDI GG'GwC_CC
Cuts at: 183 183
Size: 4541
SapI GCTCTTCn'nnn_
Cuts at: 151 2564 2774 151
Size: 2413 210 1918
Fragments arranged by size:
2413 1918 210
SbfI CC_TGCA'GG
Cuts at: 1588 1588
Size: 4541
ScaI AGT'ACT
Cuts at: 4058 4058
Size: 4541
SfcI C'TryA_G
Cuts at: 33 1250 1354 1584 2269 3262 3453 33
Size: 1217 104 230 685 993 191 1121
Fragments arranged by size:
1217 1121 993 685 230 191 104
SfoI GGC'GCC
Cuts at: 202 1973 2221 202
Size: 1771 248 2522
Fragments arranged by size:
2522 1771 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4541
SmaI CCC'GGG
Cuts at: 1563 1563
Size: 4541
SmlI C'TyrA_G
Cuts at: 1406 1696 2611 3103 3365 1406
Size: 290 915 492 262 2582
Fragments arranged by size:
2582 915 492 290 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4541
SphI G_CATG'C
Cuts at: 1594 2094 1594
Size: 500 4041
StuI AGG'CCT
Cuts at: 804 804
Size: 4541
StyI C'CwwG_G
Cuts at: 1459 1675 2029 1459
Size: 216 354 3971
Fragments arranged by size:
3971 354 216
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2124 2257 2716 2925 2124
Size: 133 459 209 3740
Fragments arranged by size:
3740 459 209 133
TatI w'GTAC_w
Cuts at: 612 4056 612
Size: 3444 1097
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4058 4090 4058
Size: 32 4509
Tth111I GACn'n_nGTC
Cuts at: 2338 2338
Size: 4541
XbaI T'CTAG_A
Cuts at: 1572 1572
Size: 4541
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2036 2036
Size: 4541
XmaI C'CCGG_G
Cuts at: 1561 1561
Size: 4541
XmnI GAAnn'nnTTC
Cuts at: 1016 1016
Size: 4541
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BcgI BfrBI BglI BmtI BsaXI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PshAI PsiI PspOMI PspXI
PsrI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI ZraI