Restriction map for
pF1KA1159
(Circular) (Six-base) MAPSORT of: pF1KA1159.seq Check: 3553 from: 1 to: 3869
pF1KA1159 3869 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AccI GT'mk_AC
Cuts at: 770 907 770
Size: 137 3732
Acc65I G'GTAC_C
Cuts at: 885 885
Size: 3869
AclI AA'CG_TT
Cuts at: 3709 3709
Size: 3869
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 480 1694 2126 2873 3141 480
Size: 1214 432 747 268 1208
Fragments arranged by size:
1214 1208 747 432 268
AflIII A'CryG_T
Cuts at: 1221 2325 3302 1221
Size: 1104 977 1788
Fragments arranged by size:
1788 1104 977
AgeI A'CCGG_T
Cuts at: 2257 2257
Size: 3869
AlwNI CAG_nnn'CTG
Cuts at: 1174 2741 1174
Size: 1567 2302
ApaLI G'TGCA_C
Cuts at: 2639 2639
Size: 3869
ApoI r'AATT_y
Cuts at: 873 3222 3478 873
Size: 2349 256 1264
Fragments arranged by size:
2349 1264 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3869
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3869
AvaI C'yCGr_G
Cuts at: 239 707 889 239
Size: 468 182 3219
Fragments arranged by size:
3219 468 182
AvrII C'CTAG_G
Cuts at: 303 303
Size: 3869
BamHI G'GATC_C
Cuts at: 894 894
Size: 3869
BanI G'GyrC_C
Cuts at: 243 885 1299 1547 1582 243
Size: 642 414 248 35 2530
Fragments arranged by size:
2530 642 414 248 35
BanII G_rGCy'C
Cuts at: 727 883 1913 727
Size: 156 1030 2683
Fragments arranged by size:
2683 1030 156
BbeI G_GCGC'C
Cuts at: 1303 1551 1303
Size: 248 3621
BbvCI CC'TCA_GC
Cuts at: 384 384
Size: 3869
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 100 124 134 90
Size: 10 24 10 3825
Fragments arranged by size:
3825 24 10 10
BciVI GTATCCnnnnn_n'
Cuts at: 1767 2528 3869 1767
Size: 761 1341 1767
Fragments arranged by size:
1767 1341 761
BclI T'GATC_A
Cuts at: 1389 1389
Size: 3869
BglII A'GATC_T
Cuts at: 593 698 1384 3421 593
Size: 105 686 2037 1041
Fragments arranged by size:
2037 1041 686 105
BlpI GC'TnA_GC
Cuts at: 726 981 726
Size: 255 3614
Bme1580I G_kGCm'C
Cuts at: 557 820 1494 1587 2643 557
Size: 263 674 93 1056 1783
Fragments arranged by size:
1783 1056 674 263 93
BmrI ACTGGGnnnn_n'
Cuts at: 554 1081 1162 1497 3641 554
Size: 527 81 335 2144 782
Fragments arranged by size:
2144 782 527 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2236 2236
Size: 3869
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 756 756
Size: 3869
Bpu10I CC'TnA_GC
Cuts at: 206 384 206
Size: 178 3691
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1045 1924 2416 2714 1045
Size: 879 492 298 2200
Fragments arranged by size:
2200 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 704 3573 704
Size: 2869 1000
BsaAI yAC'GTr
Cuts at: 1222 1222
Size: 3869
BsaBI GATnn'nnATC
Cuts at: 6 598 1383 3420 6
Size: 592 785 2037 455
Fragments arranged by size:
2037 785 592 455
BsaHI Gr'CG_yC
Cuts at: 1300 1548 2250 3746 1300
Size: 248 702 1496 1423
Fragments arranged by size:
1496 1423 702 248
BsaWI w'CCGG_w
Cuts at: 1058 1579 2257 2531 2678 3089 1058
Size: 521 678 274 147 411 1838
Fragments arranged by size:
1838 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 409 439 409
Size: 30 3839
BseYI C'CCAG_C
Cuts at: 410 690 746 1294 2052 2629 3125 410
Size: 280 56 548 758 577 496 1154
Fragments arranged by size:
1154 758 577 548 496 280 56
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 163
Size: 3869
BsiEI CG_ry'CG
Cuts at: 104 1411 1457 2665 104
Size: 1307 46 1208 1308
Fragments arranged by size:
1308 1307 1208 46
BsiHKAI G_wGCw'C
Cuts at: 727 883 1661 1851 2643 727
Size: 156 778 190 792 1953
Fragments arranged by size:
1953 792 778 190 156
BsmI GAATG_Cn'
Cuts at: 338 338
Size: 3869
Bsp1286I G_dGCh'C
Cuts at: 557 727 820 883 1494 1587 1661 1851 1913
Size: 170 93 63 611 93 74 190 62
Cuts at: 1913 2643 557
Size: 730 1783
Fragments arranged by size:
1783 730 611 190 170 93 93 74
63 62
BspEI T'CCGG_A
Cuts at: 3089 3089
Size: 3869
BspHI T'CATG_A
Cuts at: 3045 3045
Size: 3869
BspMI ACCTGCnnnn'nnnn_
Cuts at: 461 919 1133 1435 1816 461
Size: 458 214 302 381 2514
Fragments arranged by size:
2514 458 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2161 2215 3699 3864 50
Size: 2111 54 1484 165 55
Fragments arranged by size:
2111 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 492 1781 492
Size: 1289 2580
BsrFI r'CCGG_y
Cuts at: 1867 2048 2257 1867
Size: 181 209 3479
Fragments arranged by size:
3479 209 181
BssSI C'ACGA_G
Cuts at: 220 550 2140 2498 220
Size: 330 1590 358 1591
Fragments arranged by size:
1591 1590 358 330
BstAPI GCAn_nnn'nTGC
Cuts at: 1246 1246
Size: 3869
BstBI TT'CG_AA
Cuts at: 2230 2230
Size: 3869
BstXI CCAn_nnnn'nTGG
Cuts at: 181 487 181
Size: 306 3563
BstYI r'GATC_y
Cuts at: 1 593 698 894 1361 1384 1719 1965 2977
Size: 592 105 196 467 23 335 246 1012
Cuts at: 2977 3063 3075 3421 1
Size: 86 12 346 449
Fragments arranged by size:
1012 592 467 449 346 335 246 196
105 86 23 12
BtgI C'CryG_G
Cuts at: 284 480 284
Size: 196 3673
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 759 1819 2000 2163 759
Size: 1060 181 163 2465
Fragments arranged by size:
2465 1060 181 163
BtsI GCAGTG_nn'
Cuts at: 1112 1112
Size: 3869
DraI TTT'AAA
Cuts at: 868 868
Size: 3869
DrdI GACnn_nn'nnGTC
Cuts at: 1575 2433 1575
Size: 858 3011
EaeI y'GGCC_r
Cuts at: 171 287 1454 1628 2019 2046 3391 171
Size: 116 1167 174 391 27 1345 649
Fragments arranged by size:
1345 1167 649 391 174 116 27
EagI C'GGCC_G
Cuts at: 1454 1454
Size: 3869
EarI CTCTTCn'nnn_
Cuts at: 1892 2102 1892
Size: 210 3659
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2077 2387 2533 3679 2077
Size: 310 146 1146 2267
Fragments arranged by size:
2267 1146 310 146
EcoICRI GAG'CTC
Cuts at: 725 881 725
Size: 156 3713
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 480 756 1694 2126 2873 3141 480
Size: 276 938 432 747 268 1208
Fragments arranged by size:
1208 938 747 432 276 268
EcoNI CCTnn'n_nnAGG
Cuts at: 142 142
Size: 3869
EcoO109I rG'GnC_Cy
Cuts at: 201 491 1008 201
Size: 290 517 3062
Fragments arranged by size:
3062 517 290
EcoRI G'AATT_C
Cuts at: 873 873
Size: 3869
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 420 1888 420
Size: 1468 2401
FspI TGC'GCA
Cuts at: 1650 1650
Size: 3869
HaeII r_GCGC'y
Cuts at: 1303 1551 2573 3552 1303
Size: 248 1022 979 1620
Fragments arranged by size:
1620 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 409 441 1711 1741 1743 1773 409
Size: 32 1270 30 2 30 2505
Fragments arranged by size:
2505 1270 32 30 30 2
HincII GTy'rAC
Cuts at: 908 908
Size: 3869
KasI G'GCGC_C
Cuts at: 1299 1547 1299
Size: 248 3621
KpnI G_GTAC'C
Cuts at: 889 889
Size: 3869
MluI A'CGCG_T
Cuts at: 3302 3302
Size: 3869
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 452 534 2540 2724 452
Size: 82 2006 184 1597
Fragments arranged by size:
2006 1597 184 82
MscI TGG'CCA
Cuts at: 173 289 1630 173
Size: 116 1341 2412
Fragments arranged by size:
2412 1341 116
MslI CAynn'nnrTG
Cuts at: 485 1858 1985 485
Size: 1373 127 2369
Fragments arranged by size:
2369 1373 127
MspA1I CmG'CkG
Cuts at: 980 1294 1654 2667 2912 980
Size: 314 360 1013 245 1937
Fragments arranged by size:
1937 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2050 2050
Size: 3869
NarI GG'CG_CC
Cuts at: 1300 1548 1300
Size: 248 3621
NcoI C'CATG_G
Cuts at: 284 480 284
Size: 196 3673
NgoMIV G'CCGG_C
Cuts at: 2048 2048
Size: 3869
NspI r_CATG'y
Cuts at: 922 1422 2329 922
Size: 500 907 2462
Fragments arranged by size:
2462 907 500
PciI A'CATG_T
Cuts at: 2325 2325
Size: 3869
PmeI GTTT'AAAC
Cuts at: 868 868
Size: 3869
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3008 3040 3008
Size: 32 3837
PpuMI rG'GwC_Cy
Cuts at: 201 491 201
Size: 290 3579
PstI C_TGCA'G
Cuts at: 916 1601 916
Size: 685 3184
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3869
PvuII CAG'CTG
Cuts at: 1294 1654 1294
Size: 360 3509
RsrII CG'GwC_CG
Cuts at: 2064 2064
Size: 3869
SacI G_AGCT'C
Cuts at: 727 883 727
Size: 156 3713
SalI G'TCGA_C
Cuts at: 906 906
Size: 3869
SapI GCTCTTCn'nnn_
Cuts at: 1892 2102 1892
Size: 210 3659
SbfI CC_TGCA'GG
Cuts at: 916 916
Size: 3869
ScaI AGT'ACT
Cuts at: 3386 3386
Size: 3869
SfcI C'TryA_G
Cuts at: 33 569 783 912 1597 2590 2781 33
Size: 536 214 129 685 993 191 1121
Fragments arranged by size:
1121 993 685 536 214 191 129
SfoI GGC'GCC
Cuts at: 1301 1549 1301
Size: 248 3621
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3869
SmaI CCC'GGG
Cuts at: 241 891 241
Size: 650 3219
SmlI C'TyrA_G
Cuts at: 1024 1939 2431 2693 1024
Size: 915 492 262 2200
Fragments arranged by size:
2200 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3869
SphI G_CATG'C
Cuts at: 922 1422 922
Size: 500 3369
StuI AGG'CCT
Cuts at: 613 613
Size: 3869
StyI C'CwwG_G
Cuts at: 284 303 389 480 608 1003 1357 284
Size: 19 86 91 128 395 354 2796
Fragments arranged by size:
2796 395 354 128 91 86 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 697 1452 1585 2044 2253 697
Size: 755 133 459 209 2313
Fragments arranged by size:
2313 755 459 209 133
TatI w'GTAC_w
Cuts at: 3384 3384
Size: 3869
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3386 3418 3386
Size: 32 3837
Tth111I GACn'n_nGTC
Cuts at: 1666 1666
Size: 3869
XbaI T'CTAG_A
Cuts at: 900 900
Size: 3869
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1364 1364
Size: 3869
XmaI C'CCGG_G
Cuts at: 239 889 239
Size: 650 3219
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbvCI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI BaeI BbsI BfrBI BglI BmgBI BmtI
BseRI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI