(Circular) (Six-base) MAPSORT of: pF1KA1159.seq Check: 3553 from: 1 to: 3869 pF1KA1159 3869 bp With 182 enzymes: SgfI * June 20, 2008 10:20 .. AccI GT'mk_AC Cuts at: 770 907 770 Size: 137 3732 Acc65I G'GTAC_C Cuts at: 885 885 Size: 3869 AclI AA'CG_TT Cuts at: 3709 3709 Size: 3869 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 480 1694 2126 2873 3141 480 Size: 1214 432 747 268 1208 Fragments arranged by size: 1214 1208 747 432 268 AflIII A'CryG_T Cuts at: 1221 2325 3302 1221 Size: 1104 977 1788 Fragments arranged by size: 1788 1104 977 AgeI A'CCGG_T Cuts at: 2257 2257 Size: 3869 AlwNI CAG_nnn'CTG Cuts at: 1174 2741 1174 Size: 1567 2302 ApaLI G'TGCA_C Cuts at: 2639 2639 Size: 3869 ApoI r'AATT_y Cuts at: 873 3222 3478 873 Size: 2349 256 1264 Fragments arranged by size: 2349 1264 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3869 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3869 AvaI C'yCGr_G Cuts at: 239 707 889 239 Size: 468 182 3219 Fragments arranged by size: 3219 468 182 AvrII C'CTAG_G Cuts at: 303 303 Size: 3869 BamHI G'GATC_C Cuts at: 894 894 Size: 3869 BanI G'GyrC_C Cuts at: 243 885 1299 1547 1582 243 Size: 642 414 248 35 2530 Fragments arranged by size: 2530 642 414 248 35 BanII G_rGCy'C Cuts at: 727 883 1913 727 Size: 156 1030 2683 Fragments arranged by size: 2683 1030 156 BbeI G_GCGC'C Cuts at: 1303 1551 1303 Size: 248 3621 BbvCI CC'TCA_GC Cuts at: 384 384 Size: 3869 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 90 100 124 134 90 Size: 10 24 10 3825 Fragments arranged by size: 3825 24 10 10 BciVI GTATCCnnnnn_n' Cuts at: 1767 2528 3869 1767 Size: 761 1341 1767 Fragments arranged by size: 1767 1341 761 BclI T'GATC_A Cuts at: 1389 1389 Size: 3869 BglII A'GATC_T Cuts at: 593 698 1384 3421 593 Size: 105 686 2037 1041 Fragments arranged by size: 2037 1041 686 105 BlpI GC'TnA_GC Cuts at: 726 981 726 Size: 255 3614 Bme1580I G_kGCm'C Cuts at: 557 820 1494 1587 2643 557 Size: 263 674 93 1056 1783 Fragments arranged by size: 1783 1056 674 263 93 BmrI ACTGGGnnnn_n' Cuts at: 554 1081 1162 1497 3641 554 Size: 527 81 335 2144 782 Fragments arranged by size: 2144 782 527 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2236 2236 Size: 3869 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 756 756 Size: 3869 Bpu10I CC'TnA_GC Cuts at: 206 384 206 Size: 178 3691 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1045 1924 2416 2714 1045 Size: 879 492 298 2200 Fragments arranged by size: 2200 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 704 3573 704 Size: 2869 1000 BsaAI yAC'GTr Cuts at: 1222 1222 Size: 3869 BsaBI GATnn'nnATC Cuts at: 6 598 1383 3420 6 Size: 592 785 2037 455 Fragments arranged by size: 2037 785 592 455 BsaHI Gr'CG_yC Cuts at: 1300 1548 2250 3746 1300 Size: 248 702 1496 1423 Fragments arranged by size: 1496 1423 702 248 BsaWI w'CCGG_w Cuts at: 1058 1579 2257 2531 2678 3089 1058 Size: 521 678 274 147 411 1838 Fragments arranged by size: 1838 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 409 439 409 Size: 30 3839 BseYI C'CCAG_C Cuts at: 410 690 746 1294 2052 2629 3125 410 Size: 280 56 548 758 577 496 1154 Fragments arranged by size: 1154 758 577 548 496 280 56 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 163 163 Size: 3869 BsiEI CG_ry'CG Cuts at: 104 1411 1457 2665 104 Size: 1307 46 1208 1308 Fragments arranged by size: 1308 1307 1208 46 BsiHKAI G_wGCw'C Cuts at: 727 883 1661 1851 2643 727 Size: 156 778 190 792 1953 Fragments arranged by size: 1953 792 778 190 156 BsmI GAATG_Cn' Cuts at: 338 338 Size: 3869 Bsp1286I G_dGCh'C Cuts at: 557 727 820 883 1494 1587 1661 1851 1913 Size: 170 93 63 611 93 74 190 62 Cuts at: 1913 2643 557 Size: 730 1783 Fragments arranged by size: 1783 730 611 190 170 93 93 74 63 62 BspEI T'CCGG_A Cuts at: 3089 3089 Size: 3869 BspHI T'CATG_A Cuts at: 3045 3045 Size: 3869 BspMI ACCTGCnnnn'nnnn_ Cuts at: 461 919 1133 1435 1816 461 Size: 458 214 302 381 2514 Fragments arranged by size: 2514 458 381 302 214 BsrBI CCG'CTC Cuts at: 50 2161 2215 3699 3864 50 Size: 2111 54 1484 165 55 Fragments arranged by size: 2111 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 492 1781 492 Size: 1289 2580 BsrFI r'CCGG_y Cuts at: 1867 2048 2257 1867 Size: 181 209 3479 Fragments arranged by size: 3479 209 181 BssSI C'ACGA_G Cuts at: 220 550 2140 2498 220 Size: 330 1590 358 1591 Fragments arranged by size: 1591 1590 358 330 BstAPI GCAn_nnn'nTGC Cuts at: 1246 1246 Size: 3869 BstBI TT'CG_AA Cuts at: 2230 2230 Size: 3869 BstXI CCAn_nnnn'nTGG Cuts at: 181 487 181 Size: 306 3563 BstYI r'GATC_y Cuts at: 1 593 698 894 1361 1384 1719 1965 2977 Size: 592 105 196 467 23 335 246 1012 Cuts at: 2977 3063 3075 3421 1 Size: 86 12 346 449 Fragments arranged by size: 1012 592 467 449 346 335 246 196 105 86 23 12 BtgI C'CryG_G Cuts at: 284 480 284 Size: 196 3673 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 759 1819 2000 2163 759 Size: 1060 181 163 2465 Fragments arranged by size: 2465 1060 181 163 BtsI GCAGTG_nn' Cuts at: 1112 1112 Size: 3869 DraI TTT'AAA Cuts at: 868 868 Size: 3869 DrdI GACnn_nn'nnGTC Cuts at: 1575 2433 1575 Size: 858 3011 EaeI y'GGCC_r Cuts at: 171 287 1454 1628 2019 2046 3391 171 Size: 116 1167 174 391 27 1345 649 Fragments arranged by size: 1345 1167 649 391 174 116 27 EagI C'GGCC_G Cuts at: 1454 1454 Size: 3869 EarI CTCTTCn'nnn_ Cuts at: 1892 2102 1892 Size: 210 3659 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2077 2387 2533 3679 2077 Size: 310 146 1146 2267 Fragments arranged by size: 2267 1146 310 146 EcoICRI GAG'CTC Cuts at: 725 881 725 Size: 156 3713 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 480 756 1694 2126 2873 3141 480 Size: 276 938 432 747 268 1208 Fragments arranged by size: 1208 938 747 432 276 268 EcoNI CCTnn'n_nnAGG Cuts at: 142 142 Size: 3869 EcoO109I rG'GnC_Cy Cuts at: 201 491 1008 201 Size: 290 517 3062 Fragments arranged by size: 3062 517 290 EcoRI G'AATT_C Cuts at: 873 873 Size: 3869 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 420 1888 420 Size: 1468 2401 FspI TGC'GCA Cuts at: 1650 1650 Size: 3869 HaeII r_GCGC'y Cuts at: 1303 1551 2573 3552 1303 Size: 248 1022 979 1620 Fragments arranged by size: 1620 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 409 441 1711 1741 1743 1773 409 Size: 32 1270 30 2 30 2505 Fragments arranged by size: 2505 1270 32 30 30 2 HincII GTy'rAC Cuts at: 908 908 Size: 3869 KasI G'GCGC_C Cuts at: 1299 1547 1299 Size: 248 3621 KpnI G_GTAC'C Cuts at: 889 889 Size: 3869 MluI A'CGCG_T Cuts at: 3302 3302 Size: 3869 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 452 534 2540 2724 452 Size: 82 2006 184 1597 Fragments arranged by size: 2006 1597 184 82 MscI TGG'CCA Cuts at: 173 289 1630 173 Size: 116 1341 2412 Fragments arranged by size: 2412 1341 116 MslI CAynn'nnrTG Cuts at: 485 1858 1985 485 Size: 1373 127 2369 Fragments arranged by size: 2369 1373 127 MspA1I CmG'CkG Cuts at: 980 1294 1654 2667 2912 980 Size: 314 360 1013 245 1937 Fragments arranged by size: 1937 1013 360 314 245 NaeI GCC'GGC Cuts at: 2050 2050 Size: 3869 NarI GG'CG_CC Cuts at: 1300 1548 1300 Size: 248 3621 NcoI C'CATG_G Cuts at: 284 480 284 Size: 196 3673 NgoMIV G'CCGG_C Cuts at: 2048 2048 Size: 3869 NspI r_CATG'y Cuts at: 922 1422 2329 922 Size: 500 907 2462 Fragments arranged by size: 2462 907 500 PciI A'CATG_T Cuts at: 2325 2325 Size: 3869 PmeI GTTT'AAAC Cuts at: 868 868 Size: 3869 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3008 3040 3008 Size: 32 3837 PpuMI rG'GwC_Cy Cuts at: 201 491 201 Size: 290 3579 PstI C_TGCA'G Cuts at: 916 1601 916 Size: 685 3184 PvuI CG_AT'CG Cuts at: 104 104 Size: 3869 PvuII CAG'CTG Cuts at: 1294 1654 1294 Size: 360 3509 RsrII CG'GwC_CG Cuts at: 2064 2064 Size: 3869 SacI G_AGCT'C Cuts at: 727 883 727 Size: 156 3713 SalI G'TCGA_C Cuts at: 906 906 Size: 3869 SapI GCTCTTCn'nnn_ Cuts at: 1892 2102 1892 Size: 210 3659 SbfI CC_TGCA'GG Cuts at: 916 916 Size: 3869 ScaI AGT'ACT Cuts at: 3386 3386 Size: 3869 SfcI C'TryA_G Cuts at: 33 569 783 912 1597 2590 2781 33 Size: 536 214 129 685 993 191 1121 Fragments arranged by size: 1121 993 685 536 214 191 129 SfoI GGC'GCC Cuts at: 1301 1549 1301 Size: 248 3621 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3869 SmaI CCC'GGG Cuts at: 241 891 241 Size: 650 3219 SmlI C'TyrA_G Cuts at: 1024 1939 2431 2693 1024 Size: 915 492 262 2200 Fragments arranged by size: 2200 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3869 SphI G_CATG'C Cuts at: 922 1422 922 Size: 500 3369 StuI AGG'CCT Cuts at: 613 613 Size: 3869 StyI C'CwwG_G Cuts at: 284 303 389 480 608 1003 1357 284 Size: 19 86 91 128 395 354 2796 Fragments arranged by size: 2796 395 354 128 91 86 19 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 697 1452 1585 2044 2253 697 Size: 755 133 459 209 2313 Fragments arranged by size: 2313 755 459 209 133 TatI w'GTAC_w Cuts at: 3384 3384 Size: 3869 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3386 3418 3386 Size: 32 3837 Tth111I GACn'n_nGTC Cuts at: 1666 1666 Size: 3869 XbaI T'CTAG_A Cuts at: 900 900 Size: 3869 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1364 1364 Size: 3869 XmaI C'CCGG_G Cuts at: 239 889 239 Size: 650 3219 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AlfI AloI ApaI AscI BaeI BbsI BfrBI BglI BmgBI BmtI BseRI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI