Restriction map for
pF1KA1160
(Circular) (Six-base) MAPSORT of: pF1KA1160.seq Check: 2819 from: 1 to: 4106
pF1KA1160 4106 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AccI GT'mk_AC
Cuts at: 1144 1144
Size: 4106
Acc65I G'GTAC_C
Cuts at: 1122 1122
Size: 4106
AclI AA'CG_TT
Cuts at: 3946 3946
Size: 4106
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1931 2363 2716 3378 1931
Size: 432 353 662 2659
Fragments arranged by size:
2659 662 432 353
AflIII A'CryG_T
Cuts at: 547 1458 3258 3539 547
Size: 911 1800 281 1114
Fragments arranged by size:
1800 1114 911 281
AlwNI CAG_nnn'CTG
Cuts at: 1411 2849 1411
Size: 1438 2668
ApaLI G'TGCA_C
Cuts at: 2944 2944
Size: 4106
ApoI r'AATT_y
Cuts at: 307 314 1110 3459 3715 307
Size: 7 796 2349 256 698
Fragments arranged by size:
2349 796 698 256 7
AseI AT'TA_AT
Cuts at: 20 20
Size: 4106
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4106
AvaI C'yCGr_G
Cuts at: 1126 1126
Size: 4106
BamHI G'GATC_C
Cuts at: 1131 1131
Size: 4106
BanI G'GyrC_C
Cuts at: 1079 1122 1536 1784 1819 1079
Size: 43 414 248 35 3366
Fragments arranged by size:
3366 414 248 43 35
BanII G_rGCy'C
Cuts at: 560 1068 1120 2150 560
Size: 508 52 1030 2516
Fragments arranged by size:
2516 1030 508 52
BbeI G_GCGC'C
Cuts at: 1540 1788 1540
Size: 248 3858
BbsI GAAGACnn'nnnn_
Cuts at: 198 198
Size: 4106
BciVI GTATCCnnnnn_n'
Cuts at: 2004 3060 4106 2004
Size: 1056 1046 2004
Fragments arranged by size:
2004 1056 1046
BclI T'GATC_A
Cuts at: 1626 1626
Size: 4106
BglI GCCn_nnn'nGGC
Cuts at: 136 1032 136
Size: 896 3210
BglII A'GATC_T
Cuts at: 263 964 1621 3658 263
Size: 701 657 2037 711
Fragments arranged by size:
2037 711 701 657
BlpI GC'TnA_GC
Cuts at: 161 554 1218 161
Size: 393 664 3049
Fragments arranged by size:
3049 664 393
Bme1580I G_kGCm'C
Cuts at: 1731 1824 2948 1731
Size: 93 1124 2889
Fragments arranged by size:
2889 1124 93
BmgBI CAC'GTC
Cuts at: 834 834
Size: 4106
BmrI ACTGGGnnnn_n'
Cuts at: 375 1318 1399 1734 3878 375
Size: 943 81 335 2144 603
Fragments arranged by size:
2144 943 603 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 707 2473 707
Size: 1766 2340
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 883 883
Size: 4106
Bpu10I CC'TnA_GC
Cuts at: 141 349 141
Size: 208 3898
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1282 2161 2875 3173 1282
Size: 879 714 298 2215
Fragments arranged by size:
2215 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 910 3810 910
Size: 2900 1206
BsaAI yAC'GTr
Cuts at: 550 1459 550
Size: 909 3197
BsaBI GATnn'nnATC
Cuts at: 6 745 1620 3657 6
Size: 739 875 2037 455
Fragments arranged by size:
2037 875 739 455
BsaHI Gr'CG_yC
Cuts at: 1537 1785 2487 3983 1537
Size: 248 702 1496 1660
Fragments arranged by size:
1660 1496 702 248
BsaWI w'CCGG_w
Cuts at: 374 1295 1816 2494 2905 3052 3326 374
Size: 921 521 678 411 147 274 1154
Fragments arranged by size:
1154 921 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 524 719 733 736 745 778 781 877 886
Size: 195 14 3 9 33 3 96 9
Cuts at: 886 1076 524
Size: 190 3554
Fragments arranged by size:
3554 195 190 96 33 14 9 9
3 3
BseYI C'CCAG_C
Cuts at: 387 1531 2289 2954 3362 387
Size: 1144 758 665 408 1131
Fragments arranged by size:
1144 1131 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 979 1648 1694 2924 104
Size: 875 669 46 1230 1286
Fragments arranged by size:
1286 1230 875 669 46
BsiHKAI G_wGCw'C
Cuts at: 560 1068 1120 1898 2088 2948 560
Size: 508 52 778 190 860 1718
Fragments arranged by size:
1718 860 778 508 190 52
BsmI GAATG_Cn'
Cuts at: 297 297
Size: 4106
BsmBI CGTCTCn'nnnn_
Cuts at: 237 237
Size: 4106
Bsp1286I G_dGCh'C
Cuts at: 560 1068 1120 1731 1824 1898 2088 2150 2948
Size: 508 52 611 93 74 190 62 798
Cuts at: 2948 560
Size: 1718
Fragments arranged by size:
1718 798 611 508 190 93 74 62
52
BspEI T'CCGG_A
Cuts at: 374 374
Size: 4106
BspHI T'CATG_A
Cuts at: 432 560 2538 432
Size: 128 1978 2000
Fragments arranged by size:
2000 1978 128
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1156 1370 1672 2053 1156
Size: 214 302 381 3209
Fragments arranged by size:
3209 381 302 214
BsrBI CCG'CTC
Cuts at: 50 989 2398 2452 3936 4101 50
Size: 939 1409 54 1484 165 55
Fragments arranged by size:
1484 1409 939 165 55 54
BsrDI GCAATG_nn'
Cuts at: 824 2018 824
Size: 1194 2912
BsrFI r'CCGG_y
Cuts at: 978 2104 2285 978
Size: 1126 181 2799
Fragments arranged by size:
2799 1126 181
BssSI C'ACGA_G
Cuts at: 2377 3085 2377
Size: 708 3398
BstAPI GCAn_nnn'nTGC
Cuts at: 1483 1483
Size: 4106
BstBI TT'CG_AA
Cuts at: 312 2467 312
Size: 2155 1951
BstEII G'GTnAC_C
Cuts at: 589 589
Size: 4106
BstYI r'GATC_y
Cuts at: 1 263 964 1131 1598 1621 1956 2202 2508
Size: 262 701 167 467 23 335 246 306
Cuts at: 2508 2520 2606 3658 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 701 467 449 335 306 262 246
167 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4106
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2056 2237 2400 2056
Size: 181 163 3762
Fragments arranged by size:
3762 181 163
BtsI GCAGTG_nn'
Cuts at: 1028 1349 1028
Size: 321 3785
ClaI AT'CG_AT
Cuts at: 572 572
Size: 4106
DraI TTT'AAA
Cuts at: 1105 1105
Size: 4106
DrdI GACnn_nn'nnGTC
Cuts at: 1812 3156 1812
Size: 1344 2762
EaeI y'GGCC_r
Cuts at: 976 980 1691 1865 2256 2283 3628 976
Size: 4 711 174 391 27 1345 1454
Fragments arranged by size:
1454 1345 711 391 174 27 4
EagI C'GGCC_G
Cuts at: 976 1691 976
Size: 715 3391
EarI CTCTTCn'nnn_
Cuts at: 164 533 710 722 731 2129 2339 164
Size: 369 177 12 9 1398 210 1931
Fragments arranged by size:
1931 1398 369 210 177 12 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2314 3056 3202 3916 2314
Size: 742 146 714 2504
Fragments arranged by size:
2504 742 714 146
EcoICRI GAG'CTC
Cuts at: 558 1066 1118 558
Size: 508 52 3546
Fragments arranged by size:
3546 508 52
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 883 1931 2363 2716 3378 883
Size: 1048 432 353 662 1611
Fragments arranged by size:
1611 1048 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 394 1245 394
Size: 851 3255
EcoRI G'AATT_C
Cuts at: 314 1110 314
Size: 796 3310
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 544 2125 544
Size: 1581 2525
FseI GG_CCGG'CC
Cuts at: 982 982
Size: 4106
FspI TGC'GCA
Cuts at: 1887 1887
Size: 4106
HaeII r_GCGC'y
Cuts at: 1540 1788 3018 3789 1540
Size: 248 1230 771 1857
Fragments arranged by size:
1857 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 236 268 754 786 1948 1978 1980 2010 236
Size: 32 486 32 1162 30 2 30 2332
Fragments arranged by size:
2332 1162 486 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1145 1145
Size: 4106
KasI G'GCGC_C
Cuts at: 1536 1784 1536
Size: 248 3858
KpnI G_GTAC'C
Cuts at: 1126 1126
Size: 4106
MluI A'CGCG_T
Cuts at: 3539 3539
Size: 4106
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 752 2865 3049 752
Size: 2113 184 1809
Fragments arranged by size:
2113 1809 184
MscI TGG'CCA
Cuts at: 1867 1867
Size: 4106
MslI CAynn'nnrTG
Cuts at: 2095 2222 2095
Size: 127 3979
MspA1I CmG'CkG
Cuts at: 165 1217 1531 1891 2675 2920 165
Size: 1052 314 360 784 245 1351
Fragments arranged by size:
1351 1052 784 360 314 245
NaeI GCC'GGC
Cuts at: 980 2287 980
Size: 1307 2799
NarI GG'CG_CC
Cuts at: 1537 1785 1537
Size: 248 3858
NcoI C'CATG_G
Cuts at: 107 107
Size: 4106
NgoMIV G'CCGG_C
Cuts at: 978 2285 978
Size: 1307 2799
NspI r_CATG'y
Cuts at: 1159 1659 3262 1159
Size: 500 1603 2003
Fragments arranged by size:
2003 1603 500
PciI A'CATG_T
Cuts at: 3258 3258
Size: 4106
PmeI GTTT'AAAC
Cuts at: 1105 1105
Size: 4106
PmlI CAC'GTG
Cuts at: 550 550
Size: 4106
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2552 2584 2552
Size: 32 4074
PpuMI rG'GwC_Cy
Cuts at: 394 394
Size: 4106
PstI C_TGCA'G
Cuts at: 926 964 1153 1838 926
Size: 38 189 685 3194
Fragments arranged by size:
3194 685 189 38
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4106
PvuII CAG'CTG
Cuts at: 165 1531 1891 165
Size: 1366 360 2380
Fragments arranged by size:
2380 1366 360
RsrII CG'GwC_CG
Cuts at: 2301 2301
Size: 4106
SacI G_AGCT'C
Cuts at: 560 1068 1120 560
Size: 508 52 3546
Fragments arranged by size:
3546 508 52
SalI G'TCGA_C
Cuts at: 1143 1143
Size: 4106
SapI GCTCTTCn'nnn_
Cuts at: 2129 2339 2129
Size: 210 3896
SbfI CC_TGCA'GG
Cuts at: 926 1153 926
Size: 227 3879
ScaI AGT'ACT
Cuts at: 472 583 3623 472
Size: 111 3040 955
Fragments arranged by size:
3040 955 111
SfcI C'TryA_G
Cuts at: 33 922 960 1149 1834 2802 2993 33
Size: 889 38 189 685 968 191 1146
Fragments arranged by size:
1146 968 889 685 191 189 38
SfiI GGCCn_nnn'nGGCC
Cuts at: 136 136
Size: 4106
SfoI GGC'GCC
Cuts at: 1538 1786 1538
Size: 248 3858
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4106
SmaI CCC'GGG
Cuts at: 1128 1128
Size: 4106
SmlI C'TyrA_G
Cuts at: 1261 2176 2890 3152 1261
Size: 915 714 262 2215
Fragments arranged by size:
2215 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4106
SphI G_CATG'C
Cuts at: 1159 1659 1159
Size: 500 3606
StyI C'CwwG_G
Cuts at: 107 1240 1594 107
Size: 1133 354 2619
Fragments arranged by size:
2619 1133 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1020 1689 1822 2281 2490 1020
Size: 669 133 459 209 2636
Fragments arranged by size:
2636 669 459 209 133
TatI w'GTAC_w
Cuts at: 214 470 581 3621 214
Size: 256 111 3040 699
Fragments arranged by size:
3040 699 256 111
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3623 3655 3623
Size: 32 4074
Tth111I GACn'n_nGTC
Cuts at: 1903 1903
Size: 4106
XbaI T'CTAG_A
Cuts at: 1137 1137
Size: 4106
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 951 1601 951
Size: 650 3456
XmaI C'CCGG_G
Cuts at: 1126 1126
Size: 4106
XmnI GAAnn'nnTTC
Cuts at: 527 527
Size: 4106
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FseI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PmeI PmlI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BaeI BbvCI BcgI BfrBI
BmtI BsaXI BsgI BsiWI BsrGI BssHII BstXI BstZ17I
Bsu36I CspCI DraIII EcoNI EcoRV FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI