Restriction map for
pF1KA1161
(Circular) (Six-base) MAPSORT of: pF1KA1161.seq Check: 2801 from: 1 to: 5153
pF1KA1161 5153 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 147 1086 1355 147
Size: 939 269 3945
Fragments arranged by size:
3945 939 269
AatII G_ACGT'C
Cuts at: 872 2030 872
Size: 1158 3995
AccI GT'mk_AC
Cuts at: 647 2111 2191 647
Size: 1464 80 3609
Fragments arranged by size:
3609 1464 80
Acc65I G'GTAC_C
Cuts at: 2169 2169
Size: 5153
AclI AA'CG_TT
Cuts at: 4993 4993
Size: 5153
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 763 1410 2978 3410 4157 4425 187
Size: 576 647 1568 432 747 268 915
Fragments arranged by size:
1568 915 747 647 576 432 268
AfeI AGC'GCT
Cuts at: 685 766 685
Size: 81 5072
AflIII A'CryG_T
Cuts at: 1067 1130 2505 3609 4586 1067
Size: 63 1375 1104 977 1634
Fragments arranged by size:
1634 1375 1104 977 63
AgeI A'CCGG_T
Cuts at: 3541 3541
Size: 5153
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1696 1696
Size: 5153
AlwNI CAG_nnn'CTG
Cuts at: 1460 2458 4025 1460
Size: 998 1567 2588
Fragments arranged by size:
2588 1567 998
ApaLI G'TGCA_C
Cuts at: 1177 3923 1177
Size: 2746 2407
ApoI r'AATT_y
Cuts at: 1111 2157 4506 4762 1111
Size: 1046 2349 256 1502
Fragments arranged by size:
2349 1502 1046 256
AscI GG'CGCG_CC
Cuts at: 981 981
Size: 5153
AseI AT'TA_AT
Cuts at: 20 20
Size: 5153
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5153
AvaI C'yCGr_G
Cuts at: 679 934 1711 2173 679
Size: 255 777 462 3659
Fragments arranged by size:
3659 777 462 255
BamHI G'GATC_C
Cuts at: 2178 2178
Size: 5153
BanI G'GyrC_C
Cuts at: 391 568 924 1851 2169 2583 2831 2866 391
Size: 177 356 927 318 414 248 35 2678
Fragments arranged by size:
2678 927 414 356 318 248 177 35
BanII G_rGCy'C
Cuts at: 184 623 865 1724 2167 3197 184
Size: 439 242 859 443 1030 2140
Fragments arranged by size:
2140 1030 859 443 439 242
BbeI G_GCGC'C
Cuts at: 395 572 2587 2835 395
Size: 177 2015 248 2713
Fragments arranged by size:
2713 2015 248 177
BbsI GAAGACnn'nnnn_
Cuts at: 327 327
Size: 5153
BciVI GTATCCnnnnn_n'
Cuts at: 3051 3812 5153 3051
Size: 761 1341 3051
Fragments arranged by size:
3051 1341 761
BclI T'GATC_A
Cuts at: 2673 2673
Size: 5153
BglI GCCn_nnn'nGGC
Cuts at: 389 608 1837 389
Size: 219 1229 3705
Fragments arranged by size:
3705 1229 219
BglII A'GATC_T
Cuts at: 2668 4705 2668
Size: 2037 3116
BlpI GC'TnA_GC
Cuts at: 841 2265 841
Size: 1424 3729
Bme1580I G_kGCm'C
Cuts at: 737 1181 1685 1712 2778 2871 3927 737
Size: 444 504 27 1066 93 1056 1963
Fragments arranged by size:
1963 1066 1056 504 444 93 27
BmrI ACTGGGnnnn_n'
Cuts at: 2365 2446 2781 4925 2365
Size: 81 335 2144 2593
Fragments arranged by size:
2593 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3520 3520
Size: 5153
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1482 2023 1482
Size: 541 4612
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1885 2329 3208 3700 3998 1885
Size: 444 879 492 298 3040
Fragments arranged by size:
3040 879 492 444 298
BsaI GGTCTCn'nnnn_
Cuts at: 941 4857 941
Size: 3916 1237
BsaAI yAC'GTr
Cuts at: 2506 2506
Size: 5153
BsaBI GATnn'nnATC
Cuts at: 6 2667 4704 6
Size: 2661 2037 455
Fragments arranged by size:
2661 2037 455
BsaHI Gr'CG_yC
Cuts at: 392 569 659 869 1151 2027 2083 2143 2584
Size: 177 90 210 282 876 56 60 441
Cuts at: 2584 2832 3534 5030 392
Size: 248 702 1496 515
Fragments arranged by size:
1496 876 702 515 441 282 248 210
177 90 60 56
BsaWI w'CCGG_w
Cuts at: 2342 2863 3541 3815 3962 4373 2342
Size: 521 678 274 147 411 3122
Fragments arranged by size:
3122 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 229 259 317 347 358 388 859 889 229
Size: 30 58 30 11 30 471 30 4493
Fragments arranged by size:
4493 471 58 30 30 30 30 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 511 642 511
Size: 131 5022
BseYI C'CCAG_C
Cuts at: 177 399 491 623 1453 1635 1685 2578 3336
Size: 222 92 132 830 182 50 893 758
Cuts at: 3336 3913 4409 177
Size: 577 496 921
Fragments arranged by size:
921 893 830 758 577 496 222 182
132 92 50
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 418 1015 418
Size: 597 4556
BsiEI CG_ry'CG
Cuts at: 104 419 710 822 1441 2111 2695 2741 3949
Size: 315 291 112 619 670 584 46 1208
Cuts at: 3949 104
Size: 1308
Fragments arranged by size:
1308 1208 670 619 584 315 291 112
46
BsiHKAI G_wGCw'C
Cuts at: 527 1181 1622 1724 2167 2945 3135 3927 527
Size: 654 441 102 443 778 190 792 1753
Fragments arranged by size:
1753 792 778 654 443 441 190 102
BsmBI CGTCTCn'nnnn_
Cuts at: 249 249
Size: 5153
Bsp1286I G_dGCh'C
Cuts at: 184 527 623 737 865 1181 1622 1685 1712
Size: 343 96 114 128 316 441 63 27
Cuts at: 1712 1724 2167 2778 2871 2945 3135 3197 3927
Size: 12 443 611 93 74 190 62 730
Cuts at: 3927 184
Size: 1410
Fragments arranged by size:
1410 730 611 443 441 343 316 190
128 114 96 93 74 63 62 27
12
BspEI T'CCGG_A
Cuts at: 4373 4373
Size: 5153
BspHI T'CATG_A
Cuts at: 4329 4329
Size: 5153
BspMI ACCTGCnnnn'nnnn_
Cuts at: 147 321 1086 1272 1355 1421 2203 2417 2719
Size: 174 765 186 83 66 782 214 302
Cuts at: 2719 3100 147
Size: 381 2200
Fragments arranged by size:
2200 782 765 381 302 214 186 174
83 66
BsrBI CCG'CTC
Cuts at: 50 353 3445 3499 4983 5148 50
Size: 303 3092 54 1484 165 55
Fragments arranged by size:
3092 1484 303 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3065 3065
Size: 5153
BsrFI r'CCGG_y
Cuts at: 817 1153 1256 1436 1466 1696 1758 2041 2085
Size: 336 103 180 30 230 62 283 44
Cuts at: 2085 3151 3332 3541 817
Size: 1066 181 209 2429
Fragments arranged by size:
2429 1066 336 283 230 209 181 180
103 62 44 30
BsrGI T'GTAC_A
Cuts at: 110 1070 110
Size: 960 4193
BssHII G'CGCG_C
Cuts at: 359 702 784 981 359
Size: 343 82 197 4531
Fragments arranged by size:
4531 343 197 82
BssSI C'ACGA_G
Cuts at: 3424 3782 3424
Size: 358 4795
BstAPI GCAn_nnn'nTGC
Cuts at: 2530 2530
Size: 5153
BstBI TT'CG_AA
Cuts at: 3514 3514
Size: 5153
BstEII G'GTnAC_C
Cuts at: 1259 1881 1894 1259
Size: 622 13 4518
Fragments arranged by size:
4518 622 13
BstXI CCAn_nnnn'nTGG
Cuts at: 537 562 603 537
Size: 25 41 5087
Fragments arranged by size:
5087 41 25
BstYI r'GATC_y
Cuts at: 1 1022 2178 2645 2668 3003 3249 4261 4347
Size: 1021 1156 467 23 335 246 1012 86
Cuts at: 4347 4359 4705 1
Size: 12 346 449
Fragments arranged by size:
1156 1021 1012 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 185 215 518 986 1250 1373 1417 1613 185
Size: 30 303 468 264 123 44 196 3725
Fragments arranged by size:
3725 468 303 264 196 123 44 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 401 656 1718 1797 3103 3284 3447 401
Size: 255 1062 79 1306 181 163 2107
Fragments arranged by size:
2107 1306 1062 255 181 163 79
BtsI GCAGTG_nn'
Cuts at: 2396 2396
Size: 5153
DraI TTT'AAA
Cuts at: 2152 2152
Size: 5153
DrdI GACnn_nn'nnGTC
Cuts at: 1456 2859 3717 1456
Size: 1403 858 2892
Fragments arranged by size:
2892 1403 858
EaeI y'GGCC_r
Cuts at: 212 416 609 707 819 1438 1745 1760 1808
Size: 204 193 98 112 619 307 15 48
Cuts at: 1808 2738 2912 3303 3330 4675 212
Size: 930 174 391 27 1345 690
Fragments arranged by size:
1345 930 690 619 391 307 204 193
174 112 98 48 27 15
EagI C'GGCC_G
Cuts at: 416 707 819 1438 2738 416
Size: 291 112 619 1300 2831
Fragments arranged by size:
2831 1300 619 291 112
EarI CTCTTCn'nnn_
Cuts at: 3176 3386 3176
Size: 210 4943
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 274 328 589 1015 1126 1522 1543 3361 3671
Size: 54 261 426 111 396 21 1818 310
Cuts at: 3671 3817 4963 274
Size: 146 1146 464
Fragments arranged by size:
1818 1146 464 426 396 310 261 146
111 54 21
EcoICRI GAG'CTC
Cuts at: 1722 2165 1722
Size: 443 4710
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 763 1410 1482 2023 2978 3410 4157 4425
Size: 576 647 72 541 955 432 747 268
Cuts at: 4425 187
Size: 915
Fragments arranged by size:
955 915 747 647 576 541 432 268
72
EcoNI CCTnn'n_nnAGG
Cuts at: 253 253
Size: 5153
EcoO109I rG'GnC_Cy
Cuts at: 2292 2292
Size: 5153
EcoRI G'AATT_C
Cuts at: 2157 2157
Size: 5153
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3172 3172
Size: 5153
FspI TGC'GCA
Cuts at: 2934 2934
Size: 5153
HaeII r_GCGC'y
Cuts at: 395 508 572 687 768 835 973 1473 1489
Size: 113 64 115 81 67 138 500 16
Cuts at: 1489 1791 2587 2835 3857 4836 395
Size: 302 796 248 1022 979 712
Fragments arranged by size:
1022 979 796 712 500 302 248 138
115 113 81 67 64 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 118 150 317 349 358 390 634 666 859
Size: 32 167 32 9 32 244 32 193
Cuts at: 859 891 1555 1587 2995 3025 3027 3057 118
Size: 32 664 32 1408 30 2 30 2214
Fragments arranged by size:
2214 1408 664 244 193 167 32 32
32 32 32 32 30 30 9 2
HincII GTy'rAC
Cuts at: 1191 2112 2192 1191
Size: 921 80 4152
Fragments arranged by size:
4152 921 80
KasI G'GCGC_C
Cuts at: 391 568 2583 2831 391
Size: 177 2015 248 2713
Fragments arranged by size:
2713 2015 248 177
KpnI G_GTAC'C
Cuts at: 2173 2173
Size: 5153
MluI A'CGCG_T
Cuts at: 4586 4586
Size: 5153
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 680 3824 4008 680
Size: 3144 184 1825
Fragments arranged by size:
3144 1825 184
MscI TGG'CCA
Cuts at: 611 1810 2914 611
Size: 1199 1104 2850
Fragments arranged by size:
2850 1199 1104
MslI CAynn'nnrTG
Cuts at: 472 560 1480 3142 3269 472
Size: 88 920 1662 127 2356
Fragments arranged by size:
2356 1662 920 127 88
MspA1I CmG'CkG
Cuts at: 174 187 217 432 1419 1443 1615 1689 1857
Size: 13 30 215 987 24 172 74 168
Cuts at: 1857 2264 2578 2938 3951 4196 174
Size: 407 314 360 1013 245 1131
Fragments arranged by size:
1131 1013 987 407 360 314 245 215
172 168 74 30 24 13
NaeI GCC'GGC
Cuts at: 819 1155 1468 1698 1760 2043 3334 819
Size: 336 313 230 62 283 1291 2638
Fragments arranged by size:
2638 1291 336 313 283 230 62
NarI GG'CG_CC
Cuts at: 392 569 2584 2832 392
Size: 177 2015 248 2713
Fragments arranged by size:
2713 2015 248 177
NgoMIV G'CCGG_C
Cuts at: 817 1153 1466 1696 1758 2041 3332 817
Size: 336 313 230 62 283 1291 2638
Fragments arranged by size:
2638 1291 336 313 283 230 62
NotI GC'GGCC_GC
Cuts at: 707 707
Size: 5153
NruI TCG'CGA
Cuts at: 1568 1568
Size: 5153
NspI r_CATG'y
Cuts at: 1071 1134 1635 2206 2706 3613 1071
Size: 63 501 571 500 907 2611
Fragments arranged by size:
2611 907 571 501 500 63
PciI A'CATG_T
Cuts at: 1067 1130 3609 1067
Size: 63 2479 2611
Fragments arranged by size:
2611 2479 63
PflMI CCAn_nnn'nTGG
Cuts at: 1460 1460
Size: 5153
PmeI GTTT'AAAC
Cuts at: 2152 2152
Size: 5153
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4292 4324 4292
Size: 32 5121
PspXI vC'TCGA_Gb
Cuts at: 679 679
Size: 5153
PstI C_TGCA'G
Cuts at: 382 2200 2885 382
Size: 1818 685 2650
Fragments arranged by size:
2650 1818 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5153
PvuII CAG'CTG
Cuts at: 2578 2938 2578
Size: 360 4793
RsrII CG'GwC_CG
Cuts at: 3348 3348
Size: 5153
SacI G_AGCT'C
Cuts at: 1724 2167 1724
Size: 443 4710
SacII CC_GC'GG
Cuts at: 188 218 1420 1616 188
Size: 30 1202 196 3725
Fragments arranged by size:
3725 1202 196 30
SalI G'TCGA_C
Cuts at: 2110 2190 2110
Size: 80 5073
SapI GCTCTTCn'nnn_
Cuts at: 3176 3386 3176
Size: 210 4943
SbfI CC_TGCA'GG
Cuts at: 2200 2200
Size: 5153
ScaI AGT'ACT
Cuts at: 4670 4670
Size: 5153
SfcI C'TryA_G
Cuts at: 33 237 378 2196 2881 3874 4065 33
Size: 204 141 1818 685 993 191 1121
Fragments arranged by size:
1818 1121 993 685 204 191 141
SfoI GGC'GCC
Cuts at: 393 570 2585 2833 393
Size: 177 2015 248 2713
Fragments arranged by size:
2713 2015 248 177
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5153
SgrAI Cr'CCGG_yG
Cuts at: 2085 2085
Size: 5153
SmaI CCC'GGG
Cuts at: 2175 2175
Size: 5153
SmlI C'TyrA_G
Cuts at: 679 1864 2308 3223 3715 3977 679
Size: 1185 444 915 492 262 1855
Fragments arranged by size:
1855 1185 915 492 444 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5153
SphI G_CATG'C
Cuts at: 1635 2206 2706 1635
Size: 571 500 4082
Fragments arranged by size:
4082 571 500
StuI AGG'CCT
Cuts at: 940 940
Size: 5153
StyI C'CwwG_G
Cuts at: 461 2287 2641 461
Size: 1826 354 2973
Fragments arranged by size:
2973 1826 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1164 1331 2736 2869 3328 3537 1164
Size: 167 1405 133 459 209 2780
Fragments arranged by size:
2780 1405 459 209 167 133
TatI w'GTAC_w
Cuts at: 110 887 1070 4668 110
Size: 777 183 3598 595
Fragments arranged by size:
3598 777 595 183
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4670 4702 4670
Size: 32 5121
Tth111I GACn'n_nGTC
Cuts at: 469 2950 469
Size: 2481 2672
XbaI T'CTAG_A
Cuts at: 2184 2184
Size: 5153
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 562 2648 562
Size: 2086 3067
XhoI C'TCGA_G
Cuts at: 679 679
Size: 5153
XmaI C'CCGG_G
Cuts at: 2173 2173
Size: 5153
ZraI GAC'GTC
Cuts at: 870 2028 870
Size: 1158 3995
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NotI NruI NspI PciI PflMI PmeI PpiI PspXI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AflII AhdI AleI AloI ApaI AvrII BaeI BbvCI
BcgI BfrBI BmgBI BmtI Bpu10I BsiWI BsmI BstZ17I
Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NsiI PacI PasI
PfoI PmlI PpuMI PshAI PsiI PspOMI PsrI SanDI
SexAI SfiI SnaBI SrfI SspI SwaI XmnI