Restriction map for
pF1KA1162
(Circular) (Six-base) MAPSORT of: pF1KA1162.seq Check: 7461 from: 1 to: 4160
pF1KA1162 4160 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 1198 1198
Size: 4160
Acc65I G'GTAC_C
Cuts at: 1176 1176
Size: 4160
AclI AA'CG_TT
Cuts at: 4000 4000
Size: 4160
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 343 1079 1985 2417 3164 3432 343
Size: 736 906 432 747 268 1071
Fragments arranged by size:
1071 906 747 736 432 268
AflIII A'CryG_T
Cuts at: 1512 2616 3593 1512
Size: 1104 977 2079
Fragments arranged by size:
2079 1104 977
AgeI A'CCGG_T
Cuts at: 2548 2548
Size: 4160
AleI CACnn'nnGTG
Cuts at: 1099 1099
Size: 4160
AlwNI CAG_nnn'CTG
Cuts at: 1465 3032 1465
Size: 1567 2593
ApaI G_GGCC'C
Cuts at: 1010 1010
Size: 4160
ApaLI G'TGCA_C
Cuts at: 2930 2930
Size: 4160
ApoI r'AATT_y
Cuts at: 280 653 1164 3513 3769 280
Size: 373 511 2349 256 671
Fragments arranged by size:
2349 671 511 373 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4160
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4160
AvaI C'yCGr_G
Cuts at: 187 1031 1180 187
Size: 844 149 3167
Fragments arranged by size:
3167 844 149
BamHI G'GATC_C
Cuts at: 1185 1185
Size: 4160
BanI G'GyrC_C
Cuts at: 1176 1590 1838 1873 1176
Size: 414 248 35 3463
Fragments arranged by size:
3463 414 248 35
BanII G_rGCy'C
Cuts at: 1010 1174 2204 1010
Size: 164 1030 2966
Fragments arranged by size:
2966 1030 164
BbeI G_GCGC'C
Cuts at: 1594 1842 1594
Size: 248 3912
BbsI GAAGACnn'nnnn_
Cuts at: 500 1115 500
Size: 615 3545
BciVI GTATCCnnnnn_n'
Cuts at: 2058 2819 4160 2058
Size: 761 1341 2058
Fragments arranged by size:
2058 1341 761
BclI T'GATC_A
Cuts at: 999 1680 999
Size: 681 3479
BglII A'GATC_T
Cuts at: 912 1675 3712 912
Size: 763 2037 1360
Fragments arranged by size:
2037 1360 763
BlpI GC'TnA_GC
Cuts at: 1272 1272
Size: 4160
Bme1580I G_kGCm'C
Cuts at: 1010 1785 1878 2934 1010
Size: 775 93 1056 2236
Fragments arranged by size:
2236 1056 775 93
BmrI ACTGGGnnnn_n'
Cuts at: 1372 1453 1788 3932 1372
Size: 81 335 2144 1600
Fragments arranged by size:
2144 1600 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2527 2527
Size: 4160
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1137 1336 2215 2707 3005 1137
Size: 199 879 492 298 2292
Fragments arranged by size:
2292 879 492 298 199
BsaI GGTCTCn'nnnn_
Cuts at: 3864 3864
Size: 4160
BsaAI yAC'GTr
Cuts at: 1513 1513
Size: 4160
BsaBI GATnn'nnATC
Cuts at: 6 1674 3711 6
Size: 1668 2037 455
Fragments arranged by size:
2037 1668 455
BsaHI Gr'CG_yC
Cuts at: 1591 1839 2541 4037 1591
Size: 248 702 1496 1714
Fragments arranged by size:
1714 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1349 1870 2548 2822 2969 3380 1349
Size: 521 678 274 147 411 2129
Fragments arranged by size:
2129 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 253 283 253
Size: 30 4130
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 205 820 856 961 964 994 1066 205
Size: 615 36 105 3 30 72 3299
Fragments arranged by size:
3299 615 105 72 36 30 3
BseYI C'CCAG_C
Cuts at: 432 1086 1585 2343 2920 3416 432
Size: 654 499 758 577 496 1176
Fragments arranged by size:
1176 758 654 577 499 496
BsiEI CG_ry'CG
Cuts at: 104 1702 1748 2956 104
Size: 1598 46 1208 1308
Fragments arranged by size:
1598 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1174 1952 2142 2934 1174
Size: 778 190 792 2400
Fragments arranged by size:
2400 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1010 1174 1785 1878 1952 2142 2204 2934 1010
Size: 164 611 93 74 190 62 730 2236
Fragments arranged by size:
2236 730 611 190 164 93 74 62
BspEI T'CCGG_A
Cuts at: 3380 3380
Size: 4160
BspHI T'CATG_A
Cuts at: 3336 3336
Size: 4160
BspMI ACCTGCnnnn'nnnn_
Cuts at: 506 1210 1424 1726 2107 506
Size: 704 214 302 381 2559
Fragments arranged by size:
2559 704 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2452 2506 3990 4155 50
Size: 2402 54 1484 165 55
Fragments arranged by size:
2402 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2072 2072
Size: 4160
BsrFI r'CCGG_y
Cuts at: 2158 2339 2548 2158
Size: 181 209 3770
Fragments arranged by size:
3770 209 181
BssSI C'ACGA_G
Cuts at: 2431 2789 2431
Size: 358 3802
BstAPI GCAn_nnn'nTGC
Cuts at: 1537 1537
Size: 4160
BstBI TT'CG_AA
Cuts at: 491 2521 491
Size: 2030 2130
BstYI r'GATC_y
Cuts at: 1 912 1185 1652 1675 2010 2256 3268 3354
Size: 911 273 467 23 335 246 1012 86
Cuts at: 3354 3366 3712 1
Size: 12 346 449
Fragments arranged by size:
1012 911 467 449 346 335 273 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1049 1049
Size: 4160
BtgI C'CryG_G
Cuts at: 107 292 107
Size: 185 3975
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 935 2110 2291 2454 935
Size: 1175 181 163 2641
Fragments arranged by size:
2641 1175 181 163
BtsI GCAGTG_nn'
Cuts at: 1403 1403
Size: 4160
DraI TTT'AAA
Cuts at: 1159 1159
Size: 4160
DrdI GACnn_nn'nnGTC
Cuts at: 1866 2724 1866
Size: 858 3302
EaeI y'GGCC_r
Cuts at: 149 408 1745 1919 2310 2337 3682 149
Size: 259 1337 174 391 27 1345 627
Fragments arranged by size:
1345 1337 627 391 259 174 27
EagI C'GGCC_G
Cuts at: 1745 1745
Size: 4160
EarI CTCTTCn'nnn_
Cuts at: 896 938 2183 2393 896
Size: 42 1245 210 2663
Fragments arranged by size:
2663 1245 210 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 454 2368 2678 2824 3970 454
Size: 1914 310 146 1146 644
Fragments arranged by size:
1914 1146 644 310 146
EcoICRI GAG'CTC
Cuts at: 1172 1172
Size: 4160
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 343 1079 1985 2417 3164 3432 343
Size: 736 906 432 747 268 1071
Fragments arranged by size:
1071 906 747 736 432 268
EcoO109I rG'GnC_Cy
Cuts at: 184 1006 1007 1299 184
Size: 822 1 292 3045
Fragments arranged by size:
3045 822 292 1
EcoRI G'AATT_C
Cuts at: 653 1164 653
Size: 511 3649
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 901 931 2179 901
Size: 30 1248 2882
Fragments arranged by size:
2882 1248 30
FspI TGC'GCA
Cuts at: 1941 1941
Size: 4160
HaeII r_GCGC'y
Cuts at: 123 145 1594 1842 2864 3843 123
Size: 22 1449 248 1022 979 440
Fragments arranged by size:
1449 1022 979 440 248 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 606 638 2002 2032 2034 2064 606
Size: 32 1364 30 2 30 2702
Fragments arranged by size:
2702 1364 32 30 30 2
HincII GTy'rAC
Cuts at: 1199 1199
Size: 4160
KasI G'GCGC_C
Cuts at: 1590 1838 1590
Size: 248 3912
KpnI G_GTAC'C
Cuts at: 1180 1180
Size: 4160
MluI A'CGCG_T
Cuts at: 3593 3593
Size: 4160
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 266 2831 3015 266
Size: 2565 184 1411
Fragments arranged by size:
2565 1411 184
MscI TGG'CCA
Cuts at: 1921 1921
Size: 4160
MslI CAynn'nnrTG
Cuts at: 1099 2149 2276 1099
Size: 1050 127 2983
Fragments arranged by size:
2983 1050 127
MspA1I CmG'CkG
Cuts at: 1090 1271 1585 1945 2958 3203 1090
Size: 181 314 360 1013 245 2047
Fragments arranged by size:
2047 1013 360 314 245 181
NaeI GCC'GGC
Cuts at: 2341 2341
Size: 4160
NarI GG'CG_CC
Cuts at: 1591 1839 1591
Size: 248 3912
NcoI C'CATG_G
Cuts at: 107 292 107
Size: 185 3975
NgoMIV G'CCGG_C
Cuts at: 2339 2339
Size: 4160
NspI r_CATG'y
Cuts at: 1141 1213 1713 2620 1141
Size: 72 500 907 2681
Fragments arranged by size:
2681 907 500 72
PciI A'CATG_T
Cuts at: 2616 2616
Size: 4160
PmeI GTTT'AAAC
Cuts at: 1159 1159
Size: 4160
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3299 3331 3299
Size: 32 4128
PspOMI G'GGCC_C
Cuts at: 1006 1006
Size: 4160
PstI C_TGCA'G
Cuts at: 503 1207 1892 503
Size: 704 685 2771
Fragments arranged by size:
2771 704 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4160
PvuII CAG'CTG
Cuts at: 1090 1585 1945 1090
Size: 495 360 3305
Fragments arranged by size:
3305 495 360
RsrII CG'GwC_CG
Cuts at: 2355 2355
Size: 4160
SacI G_AGCT'C
Cuts at: 1174 1174
Size: 4160
SalI G'TCGA_C
Cuts at: 1197 1197
Size: 4160
SapI GCTCTTCn'nnn_
Cuts at: 2183 2393 2183
Size: 210 3950
SbfI CC_TGCA'GG
Cuts at: 1207 1207
Size: 4160
ScaI AGT'ACT
Cuts at: 3677 3677
Size: 4160
SexAI A'CCwGG_T
Cuts at: 396 396
Size: 4160
SfcI C'TryA_G
Cuts at: 33 499 1203 1888 2881 3072 33
Size: 466 704 685 993 191 1121
Fragments arranged by size:
1121 993 704 685 466 191
SfoI GGC'GCC
Cuts at: 1592 1840 1592
Size: 248 3912
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4160
SmaI CCC'GGG
Cuts at: 1033 1182 1033
Size: 149 4011
SmlI C'TyrA_G
Cuts at: 1116 1315 2230 2722 2984 1116
Size: 199 915 492 262 2292
Fragments arranged by size:
2292 915 492 262 199
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4160
SphI G_CATG'C
Cuts at: 1141 1213 1713 1141
Size: 72 500 3588
Fragments arranged by size:
3588 500 72
StyI C'CwwG_G
Cuts at: 107 292 695 763 1294 1648 107
Size: 185 403 68 531 354 2619
Fragments arranged by size:
2619 531 403 354 185 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1743 1876 2335 2544 1743
Size: 133 459 209 3359
Fragments arranged by size:
3359 459 209 133
TatI w'GTAC_w
Cuts at: 3675 3675
Size: 4160
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 253 285 3677 3709 253
Size: 32 3392 32 704
Fragments arranged by size:
3392 704 32 32
Tth111I GACn'n_nGTC
Cuts at: 1957 1957
Size: 4160
XbaI T'CTAG_A
Cuts at: 1191 1191
Size: 4160
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 249 1655 249
Size: 1406 2754
XmaI C'CCGG_G
Cuts at: 1031 1180 1031
Size: 149 4011
XmnI GAAnn'nnTTC
Cuts at: 356 356
Size: 4160
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
BpmI Bpu10I BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI