Restriction map for
pF1KA1168
(Circular) (Six-base) MAPSORT of: pF1KA1168.seq Check: 3423 from: 1 to: 6947
pF1KA1168 6947 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1005 1479 3734 1005
Size: 474 2255 4218
Fragments arranged by size:
4218 2255 474
AccI GT'mk_AC
Cuts at: 2288 3284 3985 2288
Size: 996 701 5250
Fragments arranged by size:
5250 996 701
Acc65I G'GTAC_C
Cuts at: 3963 3963
Size: 6947
AclI AA'CG_TT
Cuts at: 2355 6787 2355
Size: 4432 2515
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2449 3139 3802 4772 5204 5557 6219 2449
Size: 690 663 970 432 353 662 3177
Fragments arranged by size:
3177 970 690 663 662 432 353
AflIII A'CryG_T
Cuts at: 605 2439 3155 4299 6099 6380 605
Size: 1834 716 1144 1800 281 1172
Fragments arranged by size:
1834 1800 1172 1144 716 281
AhdI GACnn_n'nnGTC
Cuts at: 1942 1942
Size: 6947
AlwNI CAG_nnn'CTG
Cuts at: 690 2238 3168 4252 5690 690
Size: 1548 930 1084 1438 1947
Fragments arranged by size:
1947 1548 1438 1084 930
ApaI G_GGCC'C
Cuts at: 3514 3514
Size: 6947
ApaLI G'TGCA_C
Cuts at: 1107 5785 1107
Size: 4678 2269
ApoI r'AATT_y
Cuts at: 997 1447 2053 2898 3951 6300 6556 997
Size: 450 606 845 1053 2349 256 1388
Fragments arranged by size:
2349 1388 1053 845 606 450 256
AseI AT'TA_AT
Cuts at: 20 977 20
Size: 957 5990
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6947
AvaI C'yCGr_G
Cuts at: 377 1794 3967 377
Size: 1417 2173 3357
Fragments arranged by size:
3357 2173 1417
BamHI G'GATC_C
Cuts at: 2848 3092 3972 2848
Size: 244 880 5823
Fragments arranged by size:
5823 880 244
BanI G'GyrC_C
Cuts at: 1020 1393 1743 3963 4377 4625 4660 1020
Size: 373 350 2220 414 248 35 3307
Fragments arranged by size:
3307 2220 414 373 350 248 35
BanII G_rGCy'C
Cuts at: 1196 1231 1274 1589 1685 3146 3514 3961 4991
Size: 35 43 315 96 1461 368 447 1030
Cuts at: 4991 1196
Size: 3152
Fragments arranged by size:
3152 1461 1030 447 368 315 96 43
35
BbeI G_GCGC'C
Cuts at: 4381 4629 4381
Size: 248 6699
BbsI GAAGACnn'nnnn_
Cuts at: 442 1509 1918 2700 442
Size: 1067 409 782 4689
Fragments arranged by size:
4689 1067 782 409
BbvCI CC'TCA_GC
Cuts at: 2258 3386 2258
Size: 1128 5819
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 486 520 1823 1857 486
Size: 34 1303 34 5576
Fragments arranged by size:
5576 1303 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2408 4845 5901 6947 2408
Size: 2437 1056 1046 2408
Fragments arranged by size:
2437 2408 1056 1046
BclI T'GATC_A
Cuts at: 1475 2277 4467 1475
Size: 802 2190 3955
Fragments arranged by size:
3955 2190 802
BfrBI ATG'CAT
Cuts at: 191 259 191
Size: 68 6879
BglI GCCn_nnn'nGGC
Cuts at: 1593 1838 1593
Size: 245 6702
BglII A'GATC_T
Cuts at: 425 2306 3815 4462 6499 425
Size: 1881 1509 647 2037 873
Fragments arranged by size:
2037 1881 1509 873 647
BlpI GC'TnA_GC
Cuts at: 3626 4059 3626
Size: 433 6514
Bme1580I G_kGCm'C
Cuts at: 1025 1111 1748 3514 3779 4572 4665 5789 1025
Size: 86 637 1766 265 793 93 1124 2183
Fragments arranged by size:
2183 1766 1124 793 637 265 93 86
BmgBI CAC'GTC
Cuts at: 120 1106 1326 2442 3156 3540 120
Size: 986 220 1116 714 384 3527
Fragments arranged by size:
3527 1116 986 714 384 220
BmrI ACTGGGnnnn_n'
Cuts at: 705 765 872 1125 1674 3663 4159 4240 4575
Size: 60 107 253 549 1989 496 81 335
Cuts at: 4575 6719 705
Size: 2144 933
Fragments arranged by size:
2144 1989 933 549 496 335 253 107
81 60
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1356 3099 5314 1356
Size: 1743 2215 2989
Fragments arranged by size:
2989 2215 1743
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 291 346 472 2083 2593 2605 3001 3118 3328
Size: 55 126 1611 510 12 396 117 210
Cuts at: 3328 291
Size: 3910
Fragments arranged by size:
3910 1611 510 396 210 126 117 55
12
Bpu10I CC'TnA_GC
Cuts at: 1230 2258 3386 1230
Size: 1028 1128 4791
Fragments arranged by size:
4791 1128 1028
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 883 1722 4123 5002 5716 6014 883
Size: 839 2401 879 714 298 1816
Fragments arranged by size:
2401 1816 879 839 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1060 6651 1060
Size: 5591 1356
BsaAI yAC'GTr
Cuts at: 4300 4300
Size: 6947
BsaBI GATnn'nnATC
Cuts at: 6 4461 6498 6
Size: 4455 2037 455
Fragments arranged by size:
4455 2037 455
BsaHI Gr'CG_yC
Cuts at: 2384 3530 4378 4626 5328 6824 2384
Size: 1146 848 248 702 1496 2507
Fragments arranged by size:
2507 1496 1146 848 702 248
BsaWI w'CCGG_w
Cuts at: 3314 3797 4136 4657 5335 5746 5893 6167 3314
Size: 483 339 521 678 411 147 274 4094
Fragments arranged by size:
4094 678 521 483 411 339 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 878 908 2542 2560 2572 2590 3421 3451 878
Size: 30 1634 18 12 18 831 30 4374
Fragments arranged by size:
4374 1634 831 30 30 18 18 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 826 886 1273 1935 3193 3244 826
Size: 60 387 662 1258 51 4529
Fragments arranged by size:
4529 1258 662 387 60 51
BseYI C'CCAG_C
Cuts at: 2036 2486 3375 3681 4372 5130 5795 6203 2036
Size: 450 889 306 691 758 665 408 2780
Fragments arranged by size:
2780 889 758 691 665 450 408 306
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 663 3757 663
Size: 3094 3853
BsiEI CG_ry'CG
Cuts at: 104 3798 4489 4535 5765 104
Size: 3694 691 46 1230 1286
Fragments arranged by size:
3694 1286 1230 691 46
BsiHKAI G_wGCw'C
Cuts at: 1077 1111 1196 1274 1496 3146 3961 4739 4929
Size: 34 85 78 222 1650 815 778 190
Cuts at: 4929 5789 1077
Size: 860 2235
Fragments arranged by size:
2235 1650 860 815 778 222 190 85
78 34
BsmI GAATG_Cn'
Cuts at: 261 3476 261
Size: 3215 3732
Bsp1286I G_dGCh'C
Cuts at: 1025 1077 1111 1196 1231 1274 1496 1589 1685
Size: 52 34 85 35 43 222 93 96
Cuts at: 1685 1748 3146 3514 3779 3961 4572 4665 4739
Size: 63 1398 368 265 182 611 93 74
Cuts at: 4739 4929 4991 5789 1025
Size: 190 62 798 2183
Fragments arranged by size:
2183 1398 798 611 368 265 222 190
182 96 93 93 85 74 63 62
52 43 35 34
BspEI T'CCGG_A
Cuts at: 3314 3314
Size: 6947
BspHI T'CATG_A
Cuts at: 473 5379 473
Size: 4906 2041
BspMI ACCTGCnnnn'nnnn_
Cuts at: 161 1005 1479 2826 3497 3734 3997 4211 4513
Size: 844 474 1347 671 237 263 214 302
Cuts at: 4513 4894 161
Size: 381 2214
Fragments arranged by size:
2214 1347 844 671 474 381 302 263
237 214
BsrBI CCG'CTC
Cuts at: 50 510 3348 3372 3432 5239 5293 6777 6942
Size: 460 2838 24 60 1807 54 1484 165
Cuts at: 6942 50
Size: 55
Fragments arranged by size:
2838 1807 1484 460 165 60 55 54
24
BsrDI GCAATG_nn'
Cuts at: 272 1552 1886 2864 3189 3398 4859 272
Size: 1280 334 978 325 209 1461 2360
Fragments arranged by size:
2360 1461 1280 978 334 325 209
BsrFI r'CCGG_y
Cuts at: 2594 3563 4945 5126 2594
Size: 969 1382 181 4415
Fragments arranged by size:
4415 1382 969 181
BsrGI T'GTAC_A
Cuts at: 356 1856 2189 356
Size: 1500 333 5114
Fragments arranged by size:
5114 1500 333
BssSI C'ACGA_G
Cuts at: 5218 5926 5218
Size: 708 6239
BstAPI GCAn_nnn'nTGC
Cuts at: 1020 2610 4324 1020
Size: 1590 1714 3643
Fragments arranged by size:
3643 1714 1590
BstBI TT'CG_AA
Cuts at: 1647 5308 1647
Size: 3661 3286
BstEII G'GTnAC_C
Cuts at: 462 3412 462
Size: 2950 3997
BstXI CCAn_nnnn'nTGG
Cuts at: 747 747
Size: 6947
BstYI r'GATC_y
Cuts at: 1 425 705 1160 2184 2306 2848 3092 3815
Size: 424 280 455 1024 122 542 244 723
Cuts at: 3815 3972 4439 4462 4797 5043 5349 5361 5447
Size: 157 467 23 335 246 306 12 86
Cuts at: 5447 6499 1
Size: 1052 449
Fragments arranged by size:
1052 1024 723 542 467 455 449 424
335 306 280 246 244 157 122 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1918 3169 1918
Size: 1251 5696
BtgI C'CryG_G
Cuts at: 1397 2072 2327 3863 1397
Size: 675 255 1536 4481
Fragments arranged by size:
4481 1536 675 255
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2940 3656 4897 5078 5241 2940
Size: 716 1241 181 163 4646
Fragments arranged by size:
4646 1241 716 181 163
BtsI GCAGTG_nn'
Cuts at: 2026 2343 2422 3637 4190 2026
Size: 317 79 1215 553 4783
Fragments arranged by size:
4783 1215 553 317 79
DraI TTT'AAA
Cuts at: 2226 3946 2226
Size: 1720 5227
DraIII CAC_nnn'GTG
Cuts at: 773 2673 773
Size: 1900 5047
DrdI GACnn_nn'nnGTC
Cuts at: 4653 5997 4653
Size: 1344 5603
EaeI y'GGCC_r
Cuts at: 749 1052 2688 3791 3929 4532 4706 5097 5124
Size: 303 1636 1103 138 603 174 391 27
Cuts at: 5124 6469 749
Size: 1345 1227
Fragments arranged by size:
1636 1345 1227 1103 603 391 303 174
138 27
EagI C'GGCC_G
Cuts at: 4532 4532
Size: 6947
EarI CTCTTCn'nnn_
Cuts at: 155 964 1079 1279 1830 2986 3199 4970 5180
Size: 809 115 200 551 1156 213 1771 210
Cuts at: 5180 155
Size: 1922
Fragments arranged by size:
1922 1771 1156 809 551 213 210 200
115
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2667 5155 5897 6043 6757 2667
Size: 2488 742 146 714 2857
Fragments arranged by size:
2857 2488 742 714 146
EcoICRI GAG'CTC
Cuts at: 1194 1272 3144 3959 1194
Size: 78 1872 815 4182
Fragments arranged by size:
4182 1872 815 78
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 291 346 472 2083 2449 2593 2605 3001 3118
Size: 55 126 1611 366 144 12 396 117
Cuts at: 3118 3139 3328 3802 4772 5204 5557 6219 291
Size: 21 189 474 970 432 353 662 1019
Fragments arranged by size:
1611 1019 970 662 474 432 396 366
353 189 144 126 117 55 21 12
EcoNI CCTnn'n_nnAGG
Cuts at: 672 1594 3424 672
Size: 922 1830 4195
Fragments arranged by size:
4195 1830 922
EcoO109I rG'GnC_Cy
Cuts at: 1716 2550 3380 3495 3510 4086 1716
Size: 834 830 115 15 576 4577
Fragments arranged by size:
4577 834 830 576 115 15
EcoRI G'AATT_C
Cuts at: 2053 3951 2053
Size: 1898 5049
EcoRV GAT'ATC
Cuts at: 1737 1737
Size: 6947
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4966 4966
Size: 6947
FspI TGC'GCA
Cuts at: 4728 4728
Size: 6947
HaeII r_GCGC'y
Cuts at: 1322 2204 3306 3578 4381 4629 5859 6630 1322
Size: 882 1102 272 803 248 1230 771 1639
Fragments arranged by size:
1639 1230 1102 882 803 771 272 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 124 156 410 442 550 582 690 697 722
Size: 32 254 32 108 32 108 7 25
Cuts at: 722 729 963 995 1059 1078 1091 1110 1721
Size: 7 234 32 64 19 13 19 611
Cuts at: 1721 1753 1928 1960 2542 2574 3272 3304 3309
Size: 32 175 32 582 32 698 32 5
Cuts at: 3309 3341 4789 4819 4821 4851 124
Size: 32 1448 30 2 30 2220
Fragments arranged by size:
2220 1448 698 611 582 254 234 175
108 108 64 32 32 32 32 32
32 32 32 32 30 30 25 19
19 13 7 7 5 2
HincII GTy'rAC
Cuts at: 834 2069 3986 834
Size: 1235 1917 3795
Fragments arranged by size:
3795 1917 1235
HpaI GTT'AAC
Cuts at: 2069 2069
Size: 6947
KasI G'GCGC_C
Cuts at: 4377 4625 4377
Size: 248 6699
KpnI G_GTAC'C
Cuts at: 3967 3967
Size: 6947
MfeI C'AATT_G
Cuts at: 2462 2462
Size: 6947
MluI A'CGCG_T
Cuts at: 6380 6380
Size: 6947
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 167 2327 5706 5890 167
Size: 2160 3379 184 1224
Fragments arranged by size:
3379 2160 1224 184
MscI TGG'CCA
Cuts at: 751 1054 3931 4708 751
Size: 303 2877 777 2990
Fragments arranged by size:
2990 2877 777 303
MslI CAynn'nnrTG
Cuts at: 341 604 841 1333 2101 2959 3077 3542 3601
Size: 263 237 492 768 858 118 465 59
Cuts at: 3601 4936 5063 341
Size: 1335 127 2225
Fragments arranged by size:
2225 1335 858 768 492 465 263 237
127 118 59
MspA1I CmG'CkG
Cuts at: 371 1011 1399 2448 3117 3625 4058 4372 4732
Size: 640 388 1049 669 508 433 314 360
Cuts at: 4732 5516 5761 371
Size: 784 245 1557
Fragments arranged by size:
1557 1049 784 669 640 508 433 388
360 314 245
NaeI GCC'GGC
Cuts at: 5128 5128
Size: 6947
NarI GG'CG_CC
Cuts at: 4378 4626 4378
Size: 248 6699
NcoI C'CATG_G
Cuts at: 2072 2327 2072
Size: 255 6692
NdeI CA'TA_TG
Cuts at: 890 2624 890
Size: 1734 5213
NgoMIV G'CCGG_C
Cuts at: 5126 5126
Size: 6947
NruI TCG'CGA
Cuts at: 1268 3407 1268
Size: 2139 4808
NsiI A_TGCA'T
Cuts at: 193 261 193
Size: 68 6879
NspI r_CATG'y
Cuts at: 609 1041 2707 3895 4000 4500 6103 609
Size: 432 1666 1188 105 500 1603 1453
Fragments arranged by size:
1666 1603 1453 1188 500 432 105
PasI CC'CwG_GG
Cuts at: 3088 3088
Size: 6947
PciI A'CATG_T
Cuts at: 605 6099 605
Size: 5494 1453
PflMI CCAn_nnn'nTGG
Cuts at: 1329 2114 2136 2451 3927 1329
Size: 785 22 315 1476 4349
Fragments arranged by size:
4349 1476 785 315 22
PfoI T'CCnGG_A
Cuts at: 617 719 1163 2060 2135 3095 617
Size: 102 444 897 75 960 4469
Fragments arranged by size:
4469 960 897 444 102 75
PmeI GTTT'AAAC
Cuts at: 3946 3946
Size: 6947
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5393 5425 5393
Size: 32 6915
PpuMI rG'GwC_Cy
Cuts at: 1716 2550 3380 3495 1716
Size: 834 830 115 5168
Fragments arranged by size:
5168 834 830 115
PspOMI G'GGCC_C
Cuts at: 3510 3510
Size: 6947
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 850 882 1528 1560 850
Size: 32 646 32 6237
Fragments arranged by size:
6237 646 32 32
PstI C_TGCA'G
Cuts at: 520 1016 1301 1490 2018 2935 3494 3994 4679
Size: 496 285 189 528 917 559 500 685
Cuts at: 4679 520
Size: 2788
Fragments arranged by size:
2788 917 685 559 528 500 496 285
189
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6947
PvuII CAG'CTG
Cuts at: 371 1011 2448 3117 3625 4372 4732 371
Size: 640 1437 669 508 747 360 2586
Fragments arranged by size:
2586 1437 747 669 640 508 360
RsrII CG'GwC_CG
Cuts at: 5142 5142
Size: 6947
SacI G_AGCT'C
Cuts at: 1196 1274 3146 3961 1196
Size: 78 1872 815 4182
Fragments arranged by size:
4182 1872 815 78
SacII CC_GC'GG
Cuts at: 1400 1400
Size: 6947
SalI G'TCGA_C
Cuts at: 3984 3984
Size: 6947
SanDI GG'GwC_CC
Cuts at: 3380 3380
Size: 6947
SapI GCTCTTCn'nnn_
Cuts at: 155 1279 2986 4970 5180 155
Size: 1124 1707 1984 210 1922
Fragments arranged by size:
1984 1922 1707 1124 210
SbfI CC_TGCA'GG
Cuts at: 1490 3494 3994 1490
Size: 2004 500 4443
Fragments arranged by size:
4443 2004 500
ScaI AGT'ACT
Cuts at: 6464 6464
Size: 6947
SexAI A'CCwGG_T
Cuts at: 3353 3353
Size: 6947
SfcI C'TryA_G
Cuts at: 33 296 516 1012 1203 1297 1486 1636 2014
Size: 263 220 496 191 94 189 150 378
Cuts at: 2014 2871 2883 2892 2931 3490 3990 4675 5643
Size: 857 12 9 39 559 500 685 968
Cuts at: 5643 5834 33
Size: 191 1146
Fragments arranged by size:
1146 968 857 685 559 500 496 378
263 220 191 191 189 150 94 39
12 9
SfoI GGC'GCC
Cuts at: 4379 4627 4379
Size: 248 6699
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6947
SmaI CCC'GGG
Cuts at: 379 3969 379
Size: 3590 3357
SmlI C'TyrA_G
Cuts at: 898 1701 4102 5017 5731 5993 898
Size: 803 2401 915 714 262 1852
Fragments arranged by size:
2401 1852 915 803 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6947
SphI G_CATG'C
Cuts at: 4000 4500 4000
Size: 500 6447
StuI AGG'CCT
Cuts at: 574 1484 1780 574
Size: 910 296 5741
Fragments arranged by size:
5741 910 296
StyI C'CwwG_G
Cuts at: 588 2072 2327 2516 3003 3425 3926 4081 4435
Size: 1484 255 189 487 422 501 155 354
Cuts at: 4435 588
Size: 3100
Fragments arranged by size:
3100 1484 501 487 422 354 255 189
155
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 784 1126 2809 3927 4530 4663 5122 5331 784
Size: 342 1683 1118 603 133 459 209 2400
Fragments arranged by size:
2400 1683 1118 603 459 342 209 133
TatI w'GTAC_w
Cuts at: 356 1133 1337 1856 2189 3590 6462 356
Size: 777 204 519 333 1401 2872 841
Fragments arranged by size:
2872 1401 841 777 519 333 204
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1957 1989 2560 2592 2659 2691 3421 3453 6464
Size: 32 571 32 67 32 730 32 3011
Cuts at: 6464 6496 1957
Size: 32 2408
Fragments arranged by size:
3011 2408 730 571 67 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 562 829 4744 562
Size: 267 3915 2765
Fragments arranged by size:
3915 2765 267
XbaI T'CTAG_A
Cuts at: 3978 3978
Size: 6947
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2568 4442 2568
Size: 1874 5073
XmaI C'CCGG_G
Cuts at: 377 3967 377
Size: 3590 3357
XmnI GAAnn'nnTTC
Cuts at: 164 164
Size: 6947
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NruI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PspOMI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AleI AlfI AloI AscI
AvrII BaeI BmtI BsiWI BsmBI BssHII BstZ17I ClaI
CspCI FseI FspAI HindIII NheI NotI PacI PmlI
PshAI PsiI PspXI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI ZraI