Restriction map for
pF1KA1179
(Circular) (Six-base) MAPSORT of: pF1KA1179.seq Check: 2328 from: 1 to: 8360
pF1KA1179 8360 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2365 2542 2365
Size: 177 8183
AccI GT'mk_AC
Cuts at: 383 4826 5398 383
Size: 4443 572 3345
Fragments arranged by size:
4443 3345 572
Acc65I G'GTAC_C
Cuts at: 628 5376 628
Size: 4748 3612
AclI AA'CG_TT
Cuts at: 1206 8200 1206
Size: 6994 1366
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 521 1596 2312 3251 3611 6185 6617 6970 7632
Size: 1075 716 939 360 2574 432 353 662
Cuts at: 7632 521
Size: 1249
Fragments arranged by size:
2574 1249 1075 939 716 662 432 360
353
AflIII A'CryG_T
Cuts at: 2945 3296 3372 5712 7512 7793 2945
Size: 351 76 2340 1800 281 3512
Fragments arranged by size:
3512 2340 1800 351 281 76
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2735 3492 2735
Size: 757 7603
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 817 849 2824 2856 817
Size: 32 1975 32 6321
Fragments arranged by size:
6321 1975 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2346 5079 5091 5665 7103 2346
Size: 2733 12 574 1438 3603
Fragments arranged by size:
3603 2733 1438 574 12
ApaI G_GGCC'C
Cuts at: 3786 3786
Size: 8360
ApaLI G'TGCA_C
Cuts at: 7198 7198
Size: 8360
ApoI r'AATT_y
Cuts at: 196 262 605 1408 3487 3577 3598 5203 5248
Size: 66 343 803 2079 90 21 1605 45
Cuts at: 5248 5302 5364 7713 7969 196
Size: 54 62 2349 256 587
Fragments arranged by size:
2349 2079 1605 803 587 343 256 90
66 62 54 45 21
AseI AT'TA_AT
Cuts at: 20 1442 2729 20
Size: 1422 1287 5651
Fragments arranged by size:
5651 1422 1287
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8360
AvaI C'yCGr_G
Cuts at: 1142 2120 2471 3265 3532 3728 5096 5380 1142
Size: 978 351 794 267 196 1368 284 4122
Fragments arranged by size:
4122 1368 978 794 351 284 267 196
AvrII C'CTAG_G
Cuts at: 1194 1194
Size: 8360
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 620 653 830 863 620
Size: 33 177 33 8117
Fragments arranged by size:
8117 177 33 33
BamHI G'GATC_C
Cuts at: 911 5385 911
Size: 4474 3886
BanI G'GyrC_C
Cuts at: 628 1546 1767 2303 2782 4992 5110 5376 5790
Size: 918 221 536 479 2210 118 266 414
Cuts at: 5790 6038 6073 628
Size: 248 35 2915
Fragments arranged by size:
2915 2210 918 536 479 414 266 248
221 118 35
BanII G_rGCy'C
Cuts at: 142 2441 2909 3786 4169 4521 4579 5157 5330
Size: 2299 468 877 383 352 58 578 173
Cuts at: 5330 5374 6404 142
Size: 44 1030 2098
Fragments arranged by size:
2299 2098 1030 877 578 468 383 352
173 58 44
BbeI G_GCGC'C
Cuts at: 5114 5794 6042 5114
Size: 680 248 7432
Fragments arranged by size:
7432 680 248
BbsI GAAGACnn'nnnn_
Cuts at: 4280 4280
Size: 8360
BbvCI CC'TCA_GC
Cuts at: 2900 3680 2900
Size: 780 7580
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 8326
BciVI GTATCCnnnnn_n'
Cuts at: 2127 4965 5170 6258 7314 8360 2127
Size: 2838 205 1088 1056 1046 2127
Fragments arranged by size:
2838 2127 1088 1056 1046 205
BclI T'GATC_A
Cuts at: 5880 5880
Size: 8360
BfrBI ATG'CAT
Cuts at: 1334 2875 2999 1334
Size: 1541 124 6695
Fragments arranged by size:
6695 1541 124
BglI GCCn_nnn'nGGC
Cuts at: 3733 5119 3733
Size: 1386 6974
BglII A'GATC_T
Cuts at: 2817 3183 5875 7912 2817
Size: 366 2692 2037 3265
Fragments arranged by size:
3265 2692 2037 366
BlpI GC'TnA_GC
Cuts at: 136 3668 5006 5472 136
Size: 3532 1338 466 3024
Fragments arranged by size:
3532 3024 1338 466
Bme1580I G_kGCm'C
Cuts at: 1021 2306 2787 3786 3869 5985 6078 7202 1021
Size: 1285 481 999 83 2116 93 1124 2179
Fragments arranged by size:
2179 2116 1285 1124 999 481 93 83
BmgBI CAC'GTC
Cuts at: 1716 2167 5295 1716
Size: 451 3128 4781
Fragments arranged by size:
4781 3128 451
BmrI ACTGGGnnnn_n'
Cuts at: 2650 3972 5058 5275 5572 5653 5988 8132 2650
Size: 1322 1086 217 297 81 335 2144 2878
Fragments arranged by size:
2878 2144 1322 1086 335 297 217 81
BmtI G_CTAG'C
Cuts at: 2410 2488 2410
Size: 78 8282
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1168 3857 4497 4651 4695 6727 1168
Size: 2689 640 154 44 2032 2801
Fragments arranged by size:
2801 2689 2032 640 154 44
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 686 1837 2335 2614 3244 3583 3898 3995 4667
Size: 1151 498 279 630 339 315 97 672
Cuts at: 4667 5101 686
Size: 434 3945
Fragments arranged by size:
3945 1151 672 630 498 434 339 315
279 97
Bpu10I CC'TnA_GC
Cuts at: 2900 3680 4250 2900
Size: 780 570 7010
Fragments arranged by size:
7010 780 570
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1138 2019 3332 3502 4291 5536 6415 7129 7427
Size: 881 1313 170 789 1245 879 714 298
Cuts at: 7427 1138
Size: 2071
Fragments arranged by size:
2071 1313 1245 881 879 789 714 298
170
BsaI GGTCTCn'nnnn_
Cuts at: 2086 2997 3783 8064 2086
Size: 911 786 4281 2382
Fragments arranged by size:
4281 2382 911 786
BsaAI yAC'GTr
Cuts at: 570 3375 5713 570
Size: 2805 2338 3217
Fragments arranged by size:
3217 2805 2338
BsaBI GATnn'nnATC
Cuts at: 6 5874 7911 6
Size: 5868 2037 455
Fragments arranged by size:
5868 2037 455
BsaHI Gr'CG_yC
Cuts at: 5111 5791 6039 6741 8237 5111
Size: 680 248 702 1496 5234
Fragments arranged by size:
5234 1496 702 680 248
BsaWI w'CCGG_w
Cuts at: 1619 2828 5019 5549 6070 6748 7159 7306 7580
Size: 1209 2191 530 521 678 411 147 274
Cuts at: 7580 1619
Size: 2399
Fragments arranged by size:
2399 2191 1209 678 530 521 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 817 847 1589 1619 1669 1699 3238 3268 3463
Size: 30 742 30 50 30 1539 30 195
Cuts at: 3463 3493 5107 5137 817
Size: 30 1614 30 4040
Fragments arranged by size:
4040 1614 1539 742 195 50 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1597 2248 2380 2692 2917 3178 3419 3757 4237
Size: 651 132 312 225 261 241 338 480
Cuts at: 4237 4708 1597
Size: 471 5249
Fragments arranged by size:
5249 651 480 471 338 312 261 241
225 132
BseYI C'CCAG_C
Cuts at: 1242 2307 2434 3435 3868 4127 4613 4811 4871
Size: 1065 127 1001 433 259 486 198 60
Cuts at: 4871 5001 5332 5785 6543 7208 7616 1242
Size: 130 331 453 758 665 408 1986
Fragments arranged by size:
1986 1065 1001 758 665 486 453 433
408 331 259 198 130 127 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 192 2722 4175 4417 192
Size: 2530 1453 242 4135
Fragments arranged by size:
4135 2530 1453 242
BsiEI CG_ry'CG
Cuts at: 104 5902 5948 7178 104
Size: 5798 46 1230 1286
Fragments arranged by size:
5798 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1154 2166 2755 2909 4169 4579 5143 5374 6152
Size: 1012 589 154 1260 410 564 231 778
Cuts at: 6152 6342 7202 1154
Size: 190 860 2312
Fragments arranged by size:
2312 1260 1012 860 778 589 564 410
231 190 154
BsmI GAATG_Cn'
Cuts at: 2229 2617 4885 2229
Size: 388 2268 5704
Fragments arranged by size:
5704 2268 388
Bsp1286I G_dGCh'C
Cuts at: 142 1021 1154 2166 2306 2441 2755 2787 2909
Size: 879 133 1012 140 135 314 32 122
Cuts at: 2909 3786 3869 4169 4521 4579 5143 5157 5330
Size: 877 83 300 352 58 564 14 173
Cuts at: 5330 5374 5985 6078 6152 6342 6404 7202 142
Size: 44 611 93 74 190 62 798 1300
Fragments arranged by size:
1300 1012 879 877 798 611 564 352
314 300 190 173 140 135 133 122
93 83 74 62 58 44 32 14
BspHI T'CATG_A
Cuts at: 1412 2621 6792 1412
Size: 1209 4171 2980
Fragments arranged by size:
4171 2980 1209
BspMI ACCTGCnnnn'nnnn_
Cuts at: 175 2365 2542 5076 5410 5624 5926 6307 175
Size: 2190 177 2534 334 214 302 381 2228
Fragments arranged by size:
2534 2228 2190 381 334 302 214 177
BsrBI CCG'CTC
Cuts at: 50 828 1821 3903 6652 6706 8190 8355 50
Size: 778 993 2082 2749 54 1484 165 55
Fragments arranged by size:
2749 2082 1484 993 778 165 55 54
BsrDI GCAATG_nn'
Cuts at: 469 1305 1991 2033 4850 6272 469
Size: 836 686 42 2817 1422 2557
Fragments arranged by size:
2817 2557 1422 836 686 42
BsrFI r'CCGG_y
Cuts at: 468 6358 6539 468
Size: 5890 181 2289
Fragments arranged by size:
5890 2289 181
BsrGI T'GTAC_A
Cuts at: 2948 3125 4506 2948
Size: 177 1381 6802
Fragments arranged by size:
6802 1381 177
BssSI C'ACGA_G
Cuts at: 2857 6631 7339 2857
Size: 3774 708 3878
Fragments arranged by size:
3878 3774 708
BstAPI GCAn_nnn'nTGC
Cuts at: 4204 4852 5091 5737 4204
Size: 648 239 646 6827
Fragments arranged by size:
6827 648 646 239
BstBI TT'CG_AA
Cuts at: 3581 6721 3581
Size: 3140 5220
BstEII G'GTnAC_C
Cuts at: 162 162
Size: 8360
BstXI CCAn_nnnn'nTGG
Cuts at: 2401 3876 3933 4135 2401
Size: 1475 57 202 6626
Fragments arranged by size:
6626 1475 202 57
BstYI r'GATC_y
Cuts at: 1 911 1288 1521 2817 3183 4260 4556 5385
Size: 910 377 233 1296 366 1077 296 829
Cuts at: 5385 5852 5875 6210 6456 6762 6774 6860 7912
Size: 467 23 335 246 306 12 86 1052
Cuts at: 7912 1
Size: 449
Fragments arranged by size:
1296 1077 1052 910 829 467 449 377
366 335 306 296 246 233 86 23
12
Bsu36I CC'TnA_GG
Cuts at: 124 143 821 124
Size: 19 678 7663
Fragments arranged by size:
7663 678 19
BtgI C'CryG_G
Cuts at: 2240 3992 4772 5066 2240
Size: 1752 780 294 5534
Fragments arranged by size:
5534 1752 780 294
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3004 6310 6491 6654 3004
Size: 3306 181 163 4710
Fragments arranged by size:
4710 3306 181 163
BtsI GCAGTG_nn'
Cuts at: 974 1234 1702 2513 2834 5603 974
Size: 260 468 811 321 2769 3731
Fragments arranged by size:
3731 2769 811 468 321 260
ClaI AT'CG_AT
Cuts at: 4202 4202
Size: 8360
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 330 365 330
Size: 35 8325
DraI TTT'AAA
Cuts at: 5359 5359
Size: 8360
DraIII CAC_nnn'GTG
Cuts at: 1027 2700 1027
Size: 1673 6687
DrdI GACnn_nn'nnGTC
Cuts at: 223 415 6066 7410 223
Size: 192 5651 1344 1173
Fragments arranged by size:
5651 1344 1173 192
EaeI y'GGCC_r
Cuts at: 466 3338 5212 5258 5945 6119 6510 6537 7882
Size: 2872 1874 46 687 174 391 27 1345
Cuts at: 7882 466
Size: 944
Fragments arranged by size:
2872 1874 1345 944 687 391 174 46
27
EagI C'GGCC_G
Cuts at: 5945 5945
Size: 8360
EarI CTCTTCn'nnn_
Cuts at: 295 929 1175 1615 3317 3578 4642 5030 6383
Size: 634 246 440 1702 261 1064 388 1353
Cuts at: 6383 6593 295
Size: 210 2062
Fragments arranged by size:
2062 1702 1353 1064 634 440 388 261
246 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2147 3148 4054 6568 7310 7456 8170 2147
Size: 1001 906 2514 742 146 714 2337
Fragments arranged by size:
2514 2337 1001 906 742 714 146
EcoICRI GAG'CTC
Cuts at: 2907 4167 4577 5372 2907
Size: 1260 410 795 5895
Fragments arranged by size:
5895 1260 795 410
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 521 686 1596 1837 2312 2335 2614 3244 3251
Size: 165 910 241 475 23 279 630 7
Cuts at: 3251 3583 3611 3898 3995 4667 5101 6185 6617
Size: 332 28 287 97 672 434 1084 432
Cuts at: 6617 6970 7632 521
Size: 353 662 1249
Fragments arranged by size:
1249 1084 910 672 662 630 475 434
432 353 332 287 279 241 165 97
28 23 7
EcoNI CCTnn'n_nnAGG
Cuts at: 1198 2527 4189 1198
Size: 1329 1662 5369
Fragments arranged by size:
5369 1662 1329
EcoO109I rG'GnC_Cy
Cuts at: 128 1102 1248 1701 1890 2589 3734 4123 5499
Size: 974 146 453 189 699 1145 389 1376
Cuts at: 5499 128
Size: 2989
Fragments arranged by size:
2989 1376 1145 974 699 453 389 189
146
EcoRI G'AATT_C
Cuts at: 605 1408 3598 5364 605
Size: 803 2190 1766 3601
Fragments arranged by size:
3601 2190 1766 803
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1329 1626 6379 1329
Size: 297 4753 3310
Fragments arranged by size:
4753 3310 297
FspI TGC'GCA
Cuts at: 3847 6141 3847
Size: 2294 6066
HaeII r_GCGC'y
Cuts at: 5114 5794 6042 7272 8043 5114
Size: 680 248 1230 771 5431
Fragments arranged by size:
5431 1230 771 680 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 250 282 646 678 1589 1621 2094 2101 2126
Size: 32 364 32 911 32 473 7 25
Cuts at: 2126 2133 2562 2594 3463 3495 6202 6232 6234
Size: 7 429 32 869 32 2707 30 2
Cuts at: 6234 6264 250
Size: 30 2346
Fragments arranged by size:
2707 2346 911 869 473 429 364 32
32 32 32 32 30 30 25 7
7 2
HincII GTy'rAC
Cuts at: 690 1091 3459 5399 690
Size: 401 2368 1940 3651
Fragments arranged by size:
3651 2368 1940 401
HpaI GTT'AAC
Cuts at: 690 690
Size: 8360
KasI G'GCGC_C
Cuts at: 5110 5790 6038 5110
Size: 680 248 7432
Fragments arranged by size:
7432 680 248
KpnI G_GTAC'C
Cuts at: 632 5380 632
Size: 4748 3612
MfeI C'AATT_G
Cuts at: 2765 3474 2765
Size: 709 7651
MluI A'CGCG_T
Cuts at: 7793 7793
Size: 8360
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 7119 7303 7119
Size: 184 8176
MscI TGG'CCA
Cuts at: 3340 5260 6121 3340
Size: 1920 861 5579
Fragments arranged by size:
5579 1920 861
MslI CAynn'nnrTG
Cuts at: 623 1640 2245 2944 3121 4439 4567 4727 6349
Size: 1017 605 699 177 1318 128 160 1622
Cuts at: 6349 6476 623
Size: 127 2507
Fragments arranged by size:
2507 1622 1318 1017 699 605 177 160
128 127
MspA1I CmG'CkG
Cuts at: 280 844 3876 4031 4063 4131 4159 4764 5076
Size: 564 3032 155 32 68 28 605 312
Cuts at: 5076 5471 5785 6145 6929 7174 280
Size: 395 314 360 784 245 1466
Fragments arranged by size:
3032 1466 784 605 564 395 360 314
312 245 155 68 32 28
NaeI GCC'GGC
Cuts at: 470 6541 470
Size: 6071 2289
NarI GG'CG_CC
Cuts at: 5111 5791 6039 5111
Size: 680 248 7432
Fragments arranged by size:
7432 680 248
NcoI C'CATG_G
Cuts at: 2240 5066 2240
Size: 2826 5534
NgoMIV G'CCGG_C
Cuts at: 468 6539 468
Size: 6071 2289
NheI G'CTAG_C
Cuts at: 2406 2484 2406
Size: 78 8282
NsiI A_TGCA'T
Cuts at: 1336 2877 3001 1336
Size: 1541 124 6695
Fragments arranged by size:
6695 1541 124
NspI r_CATG'y
Cuts at: 792 2250 2949 3300 5017 5413 5913 7516 792
Size: 1458 699 351 1717 396 500 1603 1636
Fragments arranged by size:
1717 1636 1603 1458 699 500 396 351
PasI CC'CwG_GG
Cuts at: 1389 2778 1389
Size: 1389 6971
PciI A'CATG_T
Cuts at: 2945 3296 7512 2945
Size: 351 4216 3793
Fragments arranged by size:
4216 3793 351
PflMI CCAn_nnn'nTGG
Cuts at: 2985 3048 4494 5079 2985
Size: 63 1446 585 6266
Fragments arranged by size:
6266 1446 585 63
PfoI T'CCnGG_A
Cuts at: 3443 3560 4469 4581 3443
Size: 117 909 112 7222
Fragments arranged by size:
7222 909 117 112
PmeI GTTT'AAAC
Cuts at: 5359 5359
Size: 8360
PmlI CAC'GTG
Cuts at: 3375 3375
Size: 8360
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 817 849 5024 5056 6806 6838 817
Size: 32 4175 32 1750 32 2339
Fragments arranged by size:
4175 2339 1750 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 128 1102 1248 1701 4123 128
Size: 974 146 453 2422 4365
Fragments arranged by size:
4365 2422 974 453 146
PsiI TTA'TAA
Cuts at: 3815 4295 3815
Size: 480 7880
PspOMI G'GGCC_C
Cuts at: 3782 3782
Size: 8360
PspXI vC'TCGA_Gb
Cuts at: 3265 3265
Size: 8360
PstI C_TGCA'G
Cuts at: 747 2520 2885 3417 3471 3890 4164 4746 5407
Size: 1773 365 532 54 419 274 582 661
Cuts at: 5407 6092 747
Size: 685 3015
Fragments arranged by size:
3015 1773 685 661 582 532 419 365
274 54
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8360
PvuII CAG'CTG
Cuts at: 280 844 3876 4063 4131 4159 4764 5785 6145
Size: 564 3032 187 68 28 605 1021 360
Cuts at: 6145 280
Size: 2495
Fragments arranged by size:
3032 2495 1021 605 564 360 187 68
28
RsrII CG'GwC_CG
Cuts at: 6555 6555
Size: 8360
SacI G_AGCT'C
Cuts at: 2909 4169 4579 5374 2909
Size: 1260 410 795 5895
Fragments arranged by size:
5895 1260 795 410
SalI G'TCGA_C
Cuts at: 5397 5397
Size: 8360
SanDI GG'GwC_CC
Cuts at: 1701 1701
Size: 8360
SapI GCTCTTCn'nnn_
Cuts at: 295 6383 6593 295
Size: 6088 210 2062
Fragments arranged by size:
6088 2062 210
SbfI CC_TGCA'GG
Cuts at: 2885 5407 2885
Size: 2522 5838
ScaI AGT'ACT
Cuts at: 7877 7877
Size: 8360
SexAI A'CCwGG_T
Cuts at: 2696 4655 2696
Size: 1959 6401
SfcI C'TryA_G
Cuts at: 33 743 1514 2383 2516 2821 2881 3241 3330
Size: 710 771 869 133 305 60 360 89
Cuts at: 3330 3413 3467 3886 4118 4160 4742 4823 5403
Size: 83 54 419 232 42 582 81 580
Cuts at: 5403 6088 7056 7247 33
Size: 685 968 191 1146
Fragments arranged by size:
1146 968 869 771 710 685 582 580
419 360 305 232 191 133 89 83
81 60 54 42
SfiI GGCCn_nnn'nGGCC
Cuts at: 3733 3733
Size: 8360
SfoI GGC'GCC
Cuts at: 5112 5792 6040 5112
Size: 680 248 7432
Fragments arranged by size:
7432 680 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8360
SmaI CCC'GGG
Cuts at: 2122 3730 5382 2122
Size: 1608 1652 5100
Fragments arranged by size:
5100 1652 1608
SmlI C'TyrA_G
Cuts at: 1153 1998 3265 3347 3517 4306 5515 6430 7144
Size: 845 1267 82 170 789 1209 915 714
Cuts at: 7144 7406 1153
Size: 262 2107
Fragments arranged by size:
2107 1267 1209 915 845 789 714 262
170 82
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8360
SphI G_CATG'C
Cuts at: 5413 5913 5413
Size: 500 7860
StuI AGG'CCT
Cuts at: 3328 3797 4603 5122 3328
Size: 469 806 519 6566
Fragments arranged by size:
6566 806 519 469
StyI C'CwwG_G
Cuts at: 713 947 980 1194 2240 2297 2394 3620 3737
Size: 234 33 214 1046 57 97 1226 117
Cuts at: 3737 4859 5066 5494 5848 713
Size: 1122 207 428 354 3225
Fragments arranged by size:
3225 1226 1122 1046 428 354 234 214
207 117 97 57 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 233 1852 2321 2770 2968 4327 4825 4933 5040
Size: 1619 469 449 198 1359 498 108 107
Cuts at: 5040 5943 6076 6535 6744 233
Size: 903 133 459 209 1849
Fragments arranged by size:
1849 1619 1359 903 498 469 459 449
209 198 133 108 107
TatI w'GTAC_w
Cuts at: 2948 3096 3125 3980 4506 7875 2948
Size: 148 29 855 526 3369 3433
Fragments arranged by size:
3433 3369 855 526 148 29
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1130 1162 1700 1732 2860 2892 7877 7909 1130
Size: 32 538 32 1128 32 4985 32 1581
Fragments arranged by size:
4985 1581 1128 538 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 561 3700 4186 6157 561
Size: 3139 486 1971 2764
Fragments arranged by size:
3139 2764 1971 486
XbaI T'CTAG_A
Cuts at: 4941 5391 4941
Size: 450 7910
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 735 978 2496 5855 735
Size: 243 1518 3359 3240
Fragments arranged by size:
3359 3240 1518 243
XhoI C'TCGA_G
Cuts at: 3265 3265
Size: 8360
XmaI C'CCGG_G
Cuts at: 2120 3728 5380 2120
Size: 1608 1652 5100
Fragments arranged by size:
5100 1652 1608
XmnI GAAnn'nnTTC
Cuts at: 1309 4109 5039 5248 1309
Size: 2800 930 209 4421
Fragments arranged by size:
4421 2800 930 209
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AlfI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PsiI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AleI AscI BsiWI
BsmBI BspEI BssHII BstZ17I EcoRV FseI FspAI HindIII
NdeI NotI NruI PacI PshAI PsrI SacII SgrAI
SnaBI SrfI SspI SwaI ZraI